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https://www.readbyqxmd.com/read/30305282/basal-constriction-during-midbrain-hindbrain-boundary-morphogenesis-is-mediated-by-wnt5b-and-focal-adhesion-kinase
#1
Jennifer H Gutzman, Ellie Graeden, Isabel Brachmann, Sayumi Yamazoe, James K Chen, Hazel Sive
Basal constriction occurs at the zebrafish midbrain-hindbrain boundary constriction (MHBC) and is likely a widespread morphogenetic mechanism. 3D reconstruction demonstrates that MHBC cells are wedge-shaped, and initially constrict basally, with subsequent apical expansion. wnt5b is expressed in the MHB and is required for basal constriction. Consistent with a requirement for this pathway, expression of dominant negative Gsk3β overcomes wnt5b knockdown. Immunostaining identifies focal adhesion kinase (Fak) as active in the MHB region, and knockdown demonstrates Fak is a regulator of basal constriction...
October 10, 2018: Biology Open
https://www.readbyqxmd.com/read/30166344/the-nfkb-subunit-rela-is-a-master-transcriptional-regulator-of-the-committed-epithelial-mesenchymal-transition-in-airway-epithelial-cells
#2
Bing Tian, Steven G Widen, Jun Yang, Thomas G Wood, Andrzej Kudlicki, Yingxin Zhao, Allan R Brasier
The epithelial-mesenchymal transition (EMT) is a multistep dedifferentiation program important in tissue repair. Here, we examined the role of the transcriptional regulator NFkB in EMT of human primary small airway epithelial cells (hSAECs). Surprisingly, transforming growth factor β (TGFβ) activated NFkB/RELA proto-oncogene, NFkB subunit (RELA) translocation within 1 day of stimulation, yet induction of its downstream gene regulatory network occurred only after 3 days. A time course of TGFβ-induced EMT transition was analyzed by RNA-Seq in the absence or presence of inducible shRNA-mediated silencing of RELA...
August 30, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/30055306/a-specific-haplotype-in-potential-mirnas-binding-sites-of-secreted-frizzled-related-protein-1-sfrp1-is-associated-with-bmd-variation-in-osteoporosis
#3
Fatemeh Amjadi-Moheb, Seyed Reza Hosseini, Mohadeseh Kosari-Monfared, Elham Ghadami, Hajighorban Nooreddini, Haleh Akhavan-Niaki
PURPOSE: Osteoporosis is an important multifactorial disease which is largely influenced by Wnt signaling pathway. Considering regulatory single nucleotide polymorphisms in Wnt signaling pathway may pave the road of understanding the genetic basis of predisposition to osteoporosis. The aim of this study was to determine the possible association between variants of SFRP1 and WNT5b, and osteoporosis incidence risk. METHODS: The study population comprised 186 osteoporotic patients and 118 normal subjects from Amirkola Health and Ageing Project...
November 30, 2018: Gene
https://www.readbyqxmd.com/read/29867082/planar-cell-polarity-signalling-coordinates-heart-tube-remodelling-through-tissue-scale-polarisation-of-actomyosin-activity
#4
Anne Margarete Merks, Marie Swinarski, Alexander Matthias Meyer, Nicola Victoria Müller, Ismail Özcan, Stefan Donat, Alexa Burger, Stephen Gilbert, Christian Mosimann, Salim Abdelilah-Seyfried, Daniela Panáková
Development of a multiple-chambered heart from the linear heart tube is inherently linked to cardiac looping. Although many molecular factors regulating the process of cardiac chamber ballooning have been identified, the cellular mechanisms underlying the chamber formation remain unclear. Here, we demonstrate that cardiac chambers remodel by cell neighbour exchange of cardiomyocytes guided by the planar cell polarity (PCP) pathway triggered by two non-canonical Wnt ligands, Wnt5b and Wnt11. We find that PCP signalling coordinates the localisation of actomyosin activity, and thus the efficiency of cell neighbour exchange...
June 4, 2018: Nature Communications
https://www.readbyqxmd.com/read/29847788/imp3-stabilization-of-wnt5b-mrna-facilitates-taz-activation-in-breast-cancer
#5
Sanjoy Samanta, Santosh Guru, Ameer L Elaimy, John J Amante, Jianhong Ou, Jun Yu, Lihua J Zhu, Arthur M Mercurio
Insulin-like growth factor-2 mRNA-binding protein 3 (IMP3) is an oncofetal protein associated with many aggressive cancers and implicated in the function of breast cancer stem cells (CSCs). The mechanisms involved, however, are poorly understood. We observed that IMP3 facilitates the activation of TAZ, a transcriptional co-activator of Hippo signaling that is necessary for the function of breast CSCs. The mechanism by which IMP3 activates TAZ involves both mRNA stability and transcriptional regulation. IMP3 stabilizes the mRNA of an alternative WNT ligand (WNT5B) indirectly by repressing miR145-5p, which targets WNT5B, resulting in TAZ activation by alternative WNT signaling...
May 29, 2018: Cell Reports
https://www.readbyqxmd.com/read/29678580/microrna-185-inhibits-the-growth-and-proliferation-of-osteoblasts-in-fracture-healing-by-targeting-pth-gene-through-down-regulating-wnt-%C3%AE-catenin-axis-in-an-animal-experiment
#6
Chang-Jiang Yao, Yang Lv, Cheng-Jun Zhang, Jia-Xin Jin, Li-Hu Xu, Jin Jiang, Bin Geng, Hong Li, Ya-Yi Xia, Meng Wu
Fracture healing is a repair process of a mechanical discontinuity loss of force transmission, and pathological mobility of bone. Increasing evidence suggests that microRNA (miRNA) could regulate chondrocyte, osteoblast, and osteoclast differentiation and function, indicating miRNA as key regulators of bone formation, resorption, remodeling, and repair. Hence, during this study, we established a right femur fracture mouse model to explore the effect microRNA-185 (miR-185) has on osteoblasts in mice during fracture healing and its underlying mechanism...
June 18, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29576390/role-for-wnt-signaling-in-retinal-neuropil-development-analysis-via-rna-seq-and-in-vivo-somatic-crispr-mutagenesis
#7
Sumeet Sarin, Elizabeth Zuniga-Sanchez, Yerbol Z Kurmangaliyev, Henry Cousins, Mili Patel, Jeanette Hernandez, Kelvin X Zhang, Melanie A Samuel, Marta Morey, Joshua R Sanes, S Lawrence Zipursky
Screens for genes that orchestrate neural circuit formation in mammals have been hindered by practical constraints of germline mutagenesis. To overcome these limitations, we combined RNA-seq with somatic CRISPR mutagenesis to study synapse development in the mouse retina. Here synapses occur between cellular layers, forming two multilayered neuropils. The outer neuropil, the outer plexiform layer (OPL), contains synapses made by rod and cone photoreceptor axons on rod and cone bipolar dendrites, respectively...
April 4, 2018: Neuron
https://www.readbyqxmd.com/read/29382514/-epigenetic-alterations-in-acute-lymphoblastic-leukemia
#8
REVIEW
María Del Pilar Navarrete-Meneses, Patricia Pérez-Vera
Acute lymphoblastic leukemia (ALL) is the most common childhood cancer. It is well-known that genetic alterations constitute the basis for the etiology of ALL. However, genetic abnormalities are not enough for the complete development of the disease, and additional alterations such as epigenetic modifications are required. Such alterations, like DNA methylation, histone modifications, and noncoding RNA regulation have been identified in ALL. DNA hypermethylation in promoter regions is one of the most frequent epigenetic modifications observed in ALL...
July 2017: Boletín Médico del Hospital Infantil de México
https://www.readbyqxmd.com/read/29325710/wnt5b-regulates-apoptosis-in-litopenaeus-vannamei-against-white-spot-syndrome-virus
#9
Chuanqi Wang, Lingwei Ruan, Hong Shi, Xun Xu
The Wnt signaling mediated by Wnt proteins that orchestrate and influence a myriad of cellular processes, such as cell proliferation, differentiation, tumorigenesis, apoptosis, and participation in immune defense during microbe infection. Wnt5b is one of the Wnt signaling molecules that initiate the cascade. In this study, we cloned and characterized a Wnt5b homolog from Litopenaeus vannamei designed as LvWnt5b. The full length of LvWnt5b transcript was 1726 bp with an 1107 bp open reading frame that encoded a 368 aa protein, which contained 24 discontinuous and highly conserved cysteine...
March 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29296769/wnt-ligands-contribute-to-the-immune-response-during-septic-shock-and-amplify-endotoxemia-driven-inflammation-in-mice
#10
Marcela Gatica-Andrades, Dimitrios Vagenas, Jessica Kling, Tam T K Nguyen, Helen Benham, Ranjeny Thomas, Heinrich Körner, Bala Venkatesh, Jeremy Cohen, Antje Blumenthal
Improved understanding of the molecular mechanisms underlying dysregulated inflammatory responses in severe infection and septic shock is urgently needed to improve patient management and identify new therapeutic opportunities. The WNT signaling pathway has been implicated as a novel constituent of the immune response to infection, but its contribution to the host response in septic shock is unknown. Although individual WNT proteins have been ascribed pro- or anti-inflammatory functions, their concerted contributions to inflammation in vivo remain to be clearly defined...
July 11, 2017: Blood Advances
https://www.readbyqxmd.com/read/29234131/a-gene-expression-study-of-ornamental-fin-shape-in-neolamprologus-brichardi-an-african-cichlid-species
#11
Ehsan Pashay Ahi, Florian Richter, Kristina M Sefc
The diversity of fin morphology within and across fish taxa offers great, but still largely unexplored, opportunities to investigate the proximate mechanisms underlying fin shape variation. Relying on available genetic knowledge brought forth mainly by the comprehensive study of the zebrafish caudal fin, we explored candidate molecular mechanisms for the maintenance and formation of the conspicuously elongated filaments adorning the unpaired fins of the East African "princess cichlid" Neolamprologus brichardi...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29129556/mechanisms-of-rhoa-inactivation-and-cdc42-and-rac1-activation-during-zebrafish-optic-nerve-regeneration
#12
Toru Matsukawa, Kazune Morita, Shou Omizu, Satoru Kato, Yoshiki Koriyama
When axons of the mammalian central nervous system (CNS) are injured, they fail to regenerate, while those of lower vertebrates undergo regeneration after injury. Wingless-type MMTV integration site family (Wnt) proteins play important roles in the CNS, and are reported to be activated after mammalian spinal cord or brain injury. Moreover, for axon growth to proceed, it is thought that small G-proteins, such as CDC42 and Rac1, need to be activated, whereas RhoA must be inactivated. However, the cell and molecular mechanisms involved in optic nerve regeneration remain unclear...
January 2018: Neurochemistry International
https://www.readbyqxmd.com/read/28801288/-expression-of-wnt5b-in-patients-with-hbv-related-hepatocellular-carcinoma-and-its-clinical-significance
#13
Le-Yang Xiang, Huo-Hui Ou, Zhan-Jun Chen, Ying-Hao Fang, Yu Huang, Xiang-Hong Li, Ding-Hua Yang
OBJECTIVE: To investigate the expression of Wnt5b in hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) tissues and its correlation with the clinicopathological parameters. METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemical staining were employed to measure Wnt5b mRNA and protein expressions in two groups of HBV-related HCC patients (100 cases in each) selected from a cohort of 289 cases with HBV-related HCC using simple random sampling method...
August 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28443299/data-on-the-mrna-expression-by-in-situ-hybridization-of-wnt-signaling-pathway-members-in-the-mouse-uterus
#14
Jyoti Goad, Yi-An Ko, Shafiq M Syed, Yazmin J Crossingham, Pradeep S Tanwar
Wnt signaling plays an important role in uterine organogenesis and oncogenesis. Our mRNA expression data documents the expression of various Wnt pathway members during the key stages of uterine epithelial gland development. Our data illustrates the expression of Wnt signaling inhibitors (Axin2, Sfrp2, Sfrp4, Dkk1 and Dkk3) in mice uteri at postnatal day 6 (PND 6) and day 15 (PND 15). They also describe the expression pattern of the Wnt ligands (Wnt1, Wnt2, Wnt2b, Wnt3, Wnt3a, Wnt5b, Wnt7b, Wnt8a, Wnt8b, Wnt9a, Wnt9b, Wnt10a and Wnt10b) in mice uteri with or without progesterone treatment...
June 2017: Data in Brief
https://www.readbyqxmd.com/read/28350091/clinical-implication-of-frizzled-2-expression-and-its-association-with-epithelial-to-mesenchymal-transition-in-hepatocellular-carcinoma
#15
Tomonari Asano, Suguru Yamada, Bryan C Fuchs, Hideki Takami, Masamichi Hayashi, Hiroyuki Sugimoto, Tsutomu Fujii, Kenneth K Tanabe, Yasuhiro Kodera
The epithelial-to-mesenchymal transition (EMT) is an initial, critical step in hepatocellular carcinoma (HCC) tumor invasion and metastasis. Frizzled 2 (Fzd2) expression might drive EMT through the non-canonical Wnt pathway, one of the various EMT signaling pathways. The expression of epithelial (E-cadherin) and mesenchymal (vimentin) markers, as well as that of Wnt5b, Stat3, IL-6, Jak2 and Fzd2, were measured in 15 HCC cell lines. The EMT status (vimentin to E-cadherin mRNA expression ratio), Fzd2 mRNA expression, and pSTAT3 protein expression were assessed by immunostaining in 100 HCC patients, and correlations of their expression with clinicopathological factors and prognosis were analyzed...
May 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28292423/a-wnt5-activity-asymmetry-and-intercellular-signaling-via-pcp-proteins-polarize-node-cells-for-left-right-symmetry-breaking
#16
Katsura Minegishi, Masakazu Hashimoto, Rieko Ajima, Katsuyoshi Takaoka, Kyosuke Shinohara, Yayoi Ikawa, Hiromi Nishimura, Andrew P McMahon, Karl Willert, Yasushi Okada, Hiroshi Sasaki, Dongbo Shi, Toshihiko Fujimori, Toshihisa Ohtsuka, Yasunobu Igarashi, Terry P Yamaguchi, Akihiko Shimono, Hidetaka Shiratori, Hiroshi Hamada
Polarization of node cells along the anterior-posterior axis of mouse embryos is responsible for left-right symmetry breaking. How node cells become polarized has remained unknown, however. Wnt5a and Wnt5b are expressed posteriorly relative to the node, whereas genes for Sfrp inhibitors of Wnt signaling are expressed anteriorly. Here we show that polarization of node cells is impaired in Wnt5a-/- Wnt5b-/- and Sfrp mutant embryos, and also in the presence of a uniform distribution of Wnt5a or Sfrp1, suggesting that Wnt5 and Sfrp proteins act as instructive signals in this process...
March 13, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28172680/p020-wnt5b-could-contribute-to-regeneration-of-the-epithelium-in-inflammatory-bowel-disease-by-potentiating-epithelial-mesenchymal-transition
#17
I Dotti, E Ferrer-Picόn, N Planell, E H van Herk, M Cuatrecasas, J Panés, A Salas
No abstract text is available yet for this article.
February 1, 2017: Journal of Crohn's & Colitis
https://www.readbyqxmd.com/read/28165032/gpr155-serves-as-a-predictive-biomarker-for-hematogenous-metastasis-in-patients-with-gastric-cancer
#18
Dai Shimizu, Mitsuro Kanda, Haruyoshi Tanaka, Daisuke Kobayashi, Chie Tanaka, Masamichi Hayashi, Naoki Iwata, Yukiko Niwa, Hideki Takami, Suguru Yamada, Tsutomu Fujii, Goro Nakayama, Michitaka Fujiwara, Yasuhiro Kodera
The prognosis of patients with gastric cancer (GC) with hematogenous metastasis is dismal. Identification of biomarkers specific for hematogenous metastasis is required to develop personalized treatments that improve patients' outcomes. Global expression profiling of GC tissues with synchronous hepatic metastasis without metastasis to the peritoneal cavity or distant lymph nodes was conducted using next-generation sequencing and identified the G protein-coupled receptor 155 (GPR155) as a candidate biomarker...
February 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28078366/genetic-screening-of-wnt4-and-wnt5b-in-two-populations-with-deviating-bone-mineral-densities
#19
Gretl Hendrickx, Eveline Boudin, Ellen Steenackers, Torben Leo Nielsen, Marianne Andersen, Kim Brixen, Wim Van Hul
A role for WNT4 and WNT5B in bone metabolism was indicated by genome-wide association studies (GWAS) and a Wnt4 knockout mouse model. The aim of this study was therefore to replicate and further investigate the causality between genetic variation in WNT4 and WNT5B and deviating bone mineral density (BMD) values. A WNT4 and WNT5B mutation screening was performed in patients with craniotubular hyperostosis using Sanger sequencing. Here, no putative causal mutations were detected. Moreover, a high and low BMD cohort was selected from the Odense Androgen Study population for re-sequencing...
March 2017: Calcified Tissue International
https://www.readbyqxmd.com/read/28042326/exosomes-derived-from-mir-140-5p-overexpressing-human-synovial-mesenchymal-stem-cells-enhance-cartilage-tissue-regeneration-and-prevent-osteoarthritis-of-the-knee-in-a-rat-model
#20
Shi-Cong Tao, Ting Yuan, Yue-Lei Zhang, Wen-Jing Yin, Shang-Chun Guo, Chang-Qing Zhang
OBJECTIVES: Osteoarthritis (OA) is the most common joint disease throughout the world. Exosomes derived from miR-140-5p-overexpressing synovial mesenchymal stem cells (SMSC-140s) may be effective in treating OA. We hypothesized that exosomes derived from SMSC-140 (SMSC-140-Exos) would enhance the proliferation and migration abilities of articular chondrocytes (ACs) without harming extracellular matrix (ECM) secretion. METHODS: SMSCs were transfected with or without miR-140-5p...
2017: Theranostics
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