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Tumor invasion

Mariano Di Trapani, Nicolò Manaresi, Gianni Medoro
Circulating tumor cells (CTCs) are rare cells shed into the bloodstream by invasive tumors and their analysis offers a promising noninvasive tool to predict and monitor therapeutic responses. CTCs can be isolated from patient blood and their characterization at single-cell level can inform on the genomic landscape of a tumor. All CTC enrichment methods bear a burden of contaminating normal cells, which mandate a further step of purification to enable reliable downstream genetic analysis. Here, we describe the DEPArray™ technology, a microchip-based digital sorter, which combines precise microfluidic and microelectronic enabling precise, image-based isolation of single CTCs, which can then be analyzed by Next Generation Sequencing (NGS) methods...
December 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
Yuying Liu, Hui Zhu, Zhenxue Zhang, Changchun Tu, Dongyuan Yao, Bin Wen, Ru Jiang, Xing Li, Pengfei Yi, Jiejie Zhan, Jiaping Hu, Jianwu Ding, Liping Jiang, Fanglin Zhang
Tumor suppressor genes (TSGs), including Ten-eleven translocation 1 (TET1), are hypermethylated in hepatocellular carcinoma (HCC). TET1 catalytic domain (TET1-CD) induces genome-wide DNA demethylation to activate TSGs, but so far, anticancer effects of TET1-CD are unclear. Here we showed that after HCC cells were transiently transfected with TET1-CD, the methylation levels of TSGs, namely APC, p16, RASSF1A, SOCS1 and TET1, were distinctly reduced, and their mRNA levels were significantly increased and HCC cells proliferation, migration and invasion were suppressed, but the methylation and mRNA levels of oncogenes, namely C-myc, Bmi1, EMS1, Kpna2 and c-fos, were not significantly change...
2018: PloS One
Caitlin E Jones, Anisha M Hammer, YouJin Cho, Gina M Sizemore, Edna Cukierman, Lisa D Yee, Samir N Ghadiali, Michael C Ostrowski, Jennifer L Leight
The organization of the extracellular matrix has a profound impact on cancer development and progression. The matrix becomes aligned throughout tumor progression, providing "highways" for tumor cell invasion. Aligned matrix is associated with breast density and is a negative prognostic factor in several cancers; however, the underlying mechanisms regulating this reorganization remain poorly understood. Deletion of the tumor suppressor Pten in the stroma was previously shown to promote extracellular matrix expansion and tumor progression...
December 11, 2018: Neoplasia: An International Journal for Oncology Research
Daniel Triner, Samantha N Devenport, Sadeesh K Ramakrishnan, Xiaoya Ma, Ryan A Frieler, Joel K Greenson, Naohiro Inohara, Gabriel Nunez, Justin A Colacino, Richard M Mortensen, Yatrik M Shah
BACKGROUND & AIMS: Neutrophils are among the most prevalent immune cells in the microenvironment of colon tumors, are believed to promote growth of colon tumors, and their numbers correlate with outcomes of patients with colon cancer. Trials of inhibitors of neutrophil trafficking are under way in patients with cancer, but it is not clear how neutrophils contribute to colon tumorigenesis. METHODS: Colitis-associated colon cancer was induced mice with conditional deletion of neutrophils (LysMCre;Mcl1fl/fl ) and wild-type littermates (LysMCre;Mcl1+/+ , control mice) by administration of azoxythmethane and/or dextran sulfate sodium...
December 11, 2018: Gastroenterology
Nathalie van den Tempel, Kishan A T Naipal, Anja Raams, Dik C van Gent, Martine Franckena, Joost L Boormans, Roland Kanaar
INTRODUCTION: Bladder cancer (urothelial carcinoma) is a common malignancy characterized by high recurrence rates and intense clinical follow-up, indicating the necessity for more effective therapies. Current treatment regimens include intra-vesical administration of mitomycin C (MMC) for non-muscle invasive disease and systemic cisplatin for muscle-invasive or metastatic disease. Hyperthermia, heating a tumor to 40-44°C, enhances the efficacy of these chemotherapeutics by various modes of action, one of which is inhibition of DNA repair via homologous recombination...
2018: PloS One
Zachary E Hoggarth, Danyelle B Osowski, Brooke A Freeberg, Scott H Garrett, Donald A Sens, Mary Ann Sens, Xu Dong Zhou, Ke K Zhang, Seema Somji
Muscle invasive urothelial carcinomas are divided into various molecular subtypes with basal and luminal subtypes being the prominent ones. The basal muscle-invasive urothelial carcinomas are generally more aggressive at presentation and significantly enriched with squamous features. Our laboratory has developed an in-vitro model of urothelial cancer by transforming the immortalized cell line UROtsa with arsenite (As3+) and cadmium (Cd2+). In this study, we characterized the tumors formed by these transformed cell lines as more basal-like based on their gene expression patterns with increased expression of KRT1, KRT5, KRT6, KRT14, KRT16, KRT17 and CD44...
2018: PloS One
Addison M Demer, Karl K Vance, Nikoo Cheraghi, Hilary C Reich, Peter K Lee
BACKGROUND: There are limited published data comparing wide local excision (WLE) with Mohs micrographic surgery (MMS) for the treatment of melanoma. OBJECTIVE: To describe a novel treatment algorithm for the surgical management of head and neck melanoma and compare rates of local recurrence for tumors treated with either MMS using immunohistochemistry or WLE. MATERIALS AND METHODS: A 10-year retrospective chart review including all in situ and invasive melanomas of the head and neck treated at one institution from January 2004 to June 2013...
December 13, 2018: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
Naveena Singh, Lynn Hirschowitz, Richard Zaino, Isabel Alvarado-Cabrero, Maire A Duggan, Rouba Ali-Fehmi, Elizabeth Euscher, Jonathan L Hecht, Lars-Christian Horn, Olga Ioffe, Xavier Matias-Guiu, W Glenn McCluggage, Yoshiki Mikami, Jaume Ordi, Vinita Parkash, M Ruhul Quddus, Charles M Quick, Annette Staebler, Charles Zaloudek, Marisa Nucci, Anais Malpica, Esther Oliva
Although endometrial carcinoma (EC) is generally considered to have a good prognosis, over 20% of women with EC die of their disease, with a projected increase in both incidence and mortality over the next few decades. The aim of accurate prognostication is to ensure that patients receive optimal treatment and are neither overtreated nor undertreated, thereby improving patient outcomes overall. Patients with EC can be categorized into prognostic risk groups based on clinicopathologic findings. Other than tumor type and grade, groupings and recommended management algorithms may take into account age, body mass index, stage, and presence of lymphovascular space invasion...
January 2019: International Journal of Gynecological Pathology
Suzanne M Dintzis, Stacey Hansen, Kristi M Harrington, Lennart C Tan, Dennis M Miller, Laura Ishak, Julia Parrish-Novak, David Kittle, Jeff Perry, Carolyn Gombotz, Tina Fortney, Stephanie Porenta, Lisa Hales, Kristine E Calhoun, Benjamin O Anderson, Sara H Javid, David R Byrd
CONTEXT.—: Resection of breast carcinoma with adequate margins reduces the risk of local recurrence and reoperation. Tozuleristide (BLZ-100) is an investigational peptide-fluorophore agent that may aid in intraoperative tumor detection and margin assessment. In this study, fluorescence imaging was conducted ex vivo on gross breast pathology specimens. OBJECTIVES.—: To determine the potential of tozuleristide to detect breast carcinoma in fresh pathology specimens and the feasibility of fluorescence-guided intraoperative pathology assessment of surgical margins...
December 14, 2018: Archives of Pathology & Laboratory Medicine
Y Z Liang, X L Nong
The head and neck cancer is one of the most common malignant tumors in the world. There are more than 600 000 new cases in the world every year. Cisplatin is often used in the treatment of head and neck cancer at present. However, because of its side effects and drug resistance, cisplatin often failed in the treatment of head and neck cancer. Artemisinin and its derivatives as first-line antimalarial drugs have the advantages of high efficiency, low toxicity and so on. Further study found that artemisinin and its derivatives had cytotoxic effects against head and neck cancer through inducing cell cycle arrest, inducing tumor cell apoptosis and inhibiting cell invasion and migration, oxidative damage, blocking the cell signal transduction and inhibiting angiogenesis...
October 5, 2018: Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery
Y Zhao, H Y Wang, C B Wang, M Niu, Y Y Wang, X F Li
Objective: To study the correlation between the BRAF V600E mutation and the clinicopathological features of papillary thyroid carcinoma(PTC), and to explore the application value of BRAF V600E mutation in PTC. Method: The BRAF V600E gene mutation was detected in 108 PTC cases and 30 control cases by qPCR. The results were statistically analyzed by using SPSS 21.0 software. Result: The BRAF V600E mutation was absent in the control group. The BRAF V600E mutation rate was 72.20% in 108 PTC cases. Compared with patients with wild type,the mean age of patients with the mutant BRAF V600E was significantly older( P <0...
October 5, 2018: Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery
L Z Meng, J G Fang, J H Wang, X D Yuan, F Yang, Y S Rao, Y J Feng, Y X Wei
Objective: The purpose of this study was to summarize preliminary clinical experience of transoral robotic surgery (TORS) in laryngeal and hypopharyngeal tumors with da Vinci surgical system. Method: Twelve patients were reviewed who were diagnosed with laryngeal ( n =7) and hypopharyngeal tumors ( n =5) and treated with TORS in Beijing Anzhen Hospital from July 1, 2016 to January 31, 2018. Result: Ten cases were male and two cases were female. The median age was 58 years old (ranging from 30 to 81 years)...
July 2018: Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery
Cuixia Liu, Guifeng Yang, Ni Liu, Zhenping Zhou, Bole Cao, Peng Zhou, Bin Yang
<i>Background:</i> Gastric cancer is one of the most common cancers and is the second leading cause of cancer mortality worldwide. The present study aimed to investigate the potential biological effect of long non-coding RNA (lncRNA) BNC2-AS1 on the proliferation, migration, and invasion of cervical cancer cells. <i>Methods:</i> BNC2-AS1 small interfering RNA (siRNA) was transfected into SGC7901 and BGC823 gastric cancer cell lines, with negative siRNA serving as a control. A reverse transcription-quantitative polymerase chain reaction assay was performed to confirm the knockdown of BNC2-AS1...
October 31, 2018: Clinical Laboratory
Jaqueline Carvalho de Oliveira, Luana Caroline Oliveira, Carolina Mathias, Gabrielle Araújo Pedroso, Debora Souza Lemos, Amanda Salviano-Silva, Tayana Schultz Jucoski, Sara Cristina Lobo-Alves, Erika Pereira Zambalde, Gabriel Adelman Cipolla, Daniela Fiori Gradia
AIMS: This article aims to review the most well characterized lncRNAs with important roles in hallmarks of cancer, additionally including lncRNAs with higher potential for clinical application. DISCUSSION: LncRNAs are transcripts larger than 200 nucleotides in length that do not appear to have protein-coding potential, although some of those may produce small functional peptides. These transcripts have attracted significant attention of researchers due to their role in genetic regulation including epigenetic, transcriptional, and post-transcriptional regulation, being involved in numerous biological processes as well as being associated with multifactorial diseases, including tumorigenesis...
December 14, 2018: Journal of Gene Medicine
Fernando López, Carlos Suárez, Vincent Vander Poorten, Antti Mäkitie, Iain J Nixon, Primož Strojan, Ehab Y Hanna, Juan Pablo Rodrigo, Remco de Bree, Miquel Quer, Robert P Takes, Carol R Bradford, Ashok R Shaha, Alvaro Sanabria, Alessandra Rinaldo, Alfio Ferlito
The parapharyngeal space is a complex anatomical area. Primary parapharyngeal tumors are rare tumors and 80% of them are benign. A variety of tumor types can develop in this location; most common are salivary gland neoplasm and neurogenic tumors. The management of these tumors has improved greatly owing to the developments in imaging techniques, surgery, and radiotherapy. Most tumors can be removed with a low rate of complications and recurrence. The transcervical approach is the most frequently used. In some cases, minimally invasive approaches may be used alone or in combination with a limited transcervical route, allowing large tumors to be removed by reducing morbidity of expanded approaches...
December 14, 2018: Head & Neck
Yanping Feng, Fei Liu, Guangyan Cheng, Qigen Fang, Xinyu Niu, Wei He
OBJECTIVE: To analyze the metastasis rate in intraglandular lymph nodes (IGLNs) with a focus on discussing the significance of IGLN metastasis in local control (LC) of parotid gland cancer (PGC). METHODS: A total of 337 patients were enrolled. Information including age; sex; and pathologic variables such as tumor (T) stage, IGLN metastasis, and follow-up findings was extracted and analyzed. RESULTS: IGLN metastasis was noted in 111 (32.9%) patients...
December 14, 2018: Laryngoscope
Yanfeng Liu, Lei Wu, Kai Li, Fengrui Liu, Li Wang, Dongling Zhang, Jing Zhou, Xuan Ma, Shengyu Wang, Shuanying Yang
The incidence and mortality of lung cancer ranked the first among all types of cancer in China, and non-small cell lung cancer (NSCLC) is the most common type of lung cancer accounting for 85% of all lung cancers. Given that the survival rate of patients with advanced NSCLC is still poor nowadays, identification of novel therapeutic targets and the development of effective therapies are desired for the treatment of NSCLC in clinics. In this study, we reported the upregulation of ornithine aminotransferase (OAT) in NSCLC cells and clinical tumor samples as well as its association with the advanced TNM stage, metastasis, and poor tumor differentiation of lung cancer...
December 13, 2018: Journal of Cellular Physiology
Ayca Ant, Caner Kilic, Yahya Baltu, Arzu Betul Duran, Tuncay Tunccan, Samet Ozlugedik, Nazan Bozdogan
OBJECTIVES: This study was aimed to overview the treatment protocols of lip cancer, find out a cut-off point of tumor diameter and depth of invasion (DOI) for the survival parameters. MATERIALS AND METHODS: One hundred ninety-one patients with lip SCC were studied. RESULTS: The average age of the patients was 62 years. Among 191 patients, 82.7% of the patients were men, 96.4% of them had lower lip cancer and 84% were staged as early (pT1, pT2 )...
December 13, 2018: Oral Diseases
Hassan Mohammed, Marcus Del Pero, Matthew Coates, Liam Masterson, Peter Tassone, Stuart Burrows, Ramez Nassif
OBJECTIVES/HYPOTHESIS: To present yield of transnasal esophagoscopy (TNE) biopsies of upper aerodigestive tract (UADT) lesions and define the role of TNE as a safe alternative to rigid endoscopy. STUDY DESIGN: Retrospective case series. METHODS: All patients who underwent TNE-guided biopsies attempted over a 2-year period were included. Patients were identified using coding records and outpatient diaries. Demographic data were recorded as well as the histological diagnosis and additional histological diagnostic procedures...
December 13, 2018: Laryngoscope
Nobuhiro Yamakawa, Tadaaki Kirita, Masahiro Umeda, Souichi Yanamoto, Yoshihide Ota, Mitsunobu Otsuru, Masaya Okura, Hiroshi Kurita, Shin-Ichi Yamada, Takumi Hasegawa, Tomonao Aikawa, Takahide Komori, Michihiro Ueda
BACKGROUND AND OBJECTIVES: Some patients with early-stage oral cancer have a poor prognosis owing to the delayed neck metastasis (DNM). Tumor budding is reportedly a promising prognostic marker in many cancers. Moreover, the tissue surrounding a tumor is also considered to play a prognostic role. In this study, we evaluated whether tumor budding and adjacent tissue at the invasive front can be potential novel predictors of DNM in early tongue cancer. METHODS: In total, 337 patients with early-stage tongue squamous cell carcinoma were retrospectively reviewed...
December 12, 2018: Journal of Surgical Oncology
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