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endothelial permeability

Alfredo I Sagredo, Eduardo A Sagredo, Victor Pola, César Echeverría, Rodrigo Andaur, Luis Michea, Andrés Stutzin, Felipe Simon, Katherine Marcelain, Ricardo Armisén
Transient Receptor Potential Melastatin 4 (TRPM4) is a Ca2+ -activated and voltage-dependent monovalent cation channel, which depolarizes the plasma cell membrane, thereby modulating Ca2+ influx across Ca2+ -permeable pathways. TRPM4 is involved in different physiological processes such as T cell activation and the migration of endothelial and certain immune cells. Overexpression of this channel has been reported in various types of tumors including prostate cancer. In this study, a significant overexpression of TRPM4 was found only in samples from cancer with a Gleason score higher than 7, which are more likely to spread...
October 21, 2018: Journal of Cellular Physiology
Dragan Milenkovic, Wim Vanden Berghe, Christine Morand, Sylvain Claude, Annette van de Sandt, Simone Gorressen, Laurent-Emmanuel Monfoulet, Chandra S Chirumamilla, Ken Declerck, Katarzyna Szarc Vel Szic, Maija Lahtela-Kakkonen, Clarissa Gerhauser, Marc W Merx, Malte Kelm
Although vasculo-protective effects of flavan-3-ols are widely accepted today, their impact on endothelial cell functions and molecular mechanisms of action involved is not completely understood. The aim of this study was to characterize the potential endothelium-protective effects of circulating epicatechin metabolites and to define underlying mechanisms of action by an integrated systems biology approach. Reduced leukocyte rolling over vascular endothelium was observed following epicatechin supplementation in a mouse model of inflammation...
October 19, 2018: Scientific Reports
Yang Zhang, Kun Ling Ma, Yu Xiang Gong, Gui Hua Wang, Ze Bo Hu, Liang Liu, Jian Lu, Pei Pei Chen, Chen Chen Lu, Xiong Zhong Ruan, Bi Cheng Liu
BACKGROUND: Glomerular endothelium dysfunction, which plays a crucial role in the pathogenesis of early diabetic nephropathy, might be caused by circulating metabolic abnormalities. Platelet microparticles, extracellular vesicles released from activated platelets, have recently emerged as a novel regulator of vascular dysfunction. METHODS: We studied the effects of platelet microparticles on glomerular endothelial injury in early diabetic nephropathy in rats with streptozotocin-induced diabetes and primary rat glomerular endothelial cells...
October 19, 2018: Journal of the American Society of Nephrology: JASN
Qiying Fan, Hua Mao, Liang Xie, Xinchun Pi
Acute lung injury and its more severe form, the acute respiratory distress syndrome, are life-threatening respiratory disorders. Overwhelming pulmonary inflammation and endothelium disruption are commonly observed. Endothelial cells (ECs) are well recognized as key regulators in leukocyte adhesion and migration in response to bacterial infection. Prolyl hydroxylase domain (PHD)-2 protein, a major PHD in ECs, plays a critical role in intracellular oxygen homeostasis, angiogenesis, and pulmonary hypertension...
October 16, 2018: American Journal of Pathology
Pratap Karki, Angelo Meliton, Albert Sitikov, Yufeng Tian, Tomomi Ohmura, Anna A Birukova
Exposure to particulate matter (PM) associated with air pollution remains a major public health concern, as it has been linked to significant increase in cardiopulmonary morbidity and mortality. Lung endothelial cell (EC) dysfunction is one of the hallmarks of cardiovascular events of lung exposure to PM. However, the role of PM in acute lung injury (ALI) exacerbation and delayed recovery remains incompletely understood. This study tested a hypothesis that PM augments lung injury and EC barrier dysfunction via microtubule-dependent mechanisms...
October 16, 2018: Cellular Signalling
Natascha G Alves, Andrea N Trujillo, Jerome W Breslin, Sarah Y Yuan
Excessive microvascular permeability is a serious complication following hemorrhagic shock and resuscitation (HSR). S1P has been shown to ameliorate microvascular leakage in a model of combined alcohol intoxication and hemorrhagic shock and resuscitation (HSR). In the current study, we tested the hypothesis that S1P reduces HSR-induced microvascular leakage by preserving endothelial cell junctional structure and the endothelial glycocalyx through protection of mitochondrial function. We used an established in vivo rat model of conscious HSR and assessed microvascular leakage, endothelial glycocalyx integrity, and mitochondrial function by intravital microscopy...
October 18, 2018: Shock
Sauri Hernández-Reséndiz, Mónica Muñoz-Vega, Whendy E Contreras, Gustavo E Crespo-Avilan, Julian Rodriguez-Montesinos, Oscar Arias-Carrión, Oscar Pérez-Méndez, William A Boisvert, Klaus T Preissner, Hector A Cabrera-Fuentes
One of the primary therapeutic goals of modern cardiology is to design strategies aimed at minimizing myocardial infarct size and optimizing cardiac function following acute myocardial infarction (AMI). Patients with AMI who underwent reperfusion therapy display dysfunction of the coronary endothelium. Consequently, ischemic endothelial cells become more permeable and weaken their natural anti-thrombotic and anti-inflammatory potential. Ischemia-reperfusion injury (IRI) is associated with activation of the humoral and cellular components of the hemostatic and innate immune system, and also with excessive production of reactive oxygen species (ROS), the inhibition of nitric oxide synthase, and with inflammatory processes...
August 2018: Conditioning medicine
Tianyang Xi, Feng Jin, Ying Zhu, Jialu Wang, Ling Tang, Yanzhe Wang, David S Liebeskind, Fabien Scalzo, Zhiyi He
Previous studies have reported that miR-27a-3p is down-regulated in the serum of patients with intracerebral hemorrhage (ICH).Yet the implication of miR-27a-3p down-regulation in post-ICH complications remains elusive. Here, we verified miR-27a-3p levels in the serum of ICH patients by real-time PCR and observed that miR-27a-3p is also significantly reduced in the serum of these patients. We then further investigated the effect of miR-27a-3p on post-ICH complications by intraventricular administration of a miR-27a-3p mimic in rats with collagenase-induced ICH...
October 18, 2018: Journal of Biological Chemistry
Erni J Nelwan
Dengue viral infection remains a major public health problem. As many as 400 million people are infected yearly. Even though the vaccine is available, the use of dengue vaccine is still limited due to some concerns. Among patient infected with dengue viral infection, early recognition of the virus and prompt supportive treatment are important to avoid complication and mortality.The clinical spectrum of dengue viral infection is diverse ranging from undifferentiated fever to dengue shock syndrome characterized by plasma leak and hemoconcentration...
July 2018: Acta Medica Indonesiana
Olga Simo-Servat, Cristina Hernández, Rafael Simó
BACKGROUND: Microvascular complications remain an important cause of morbidity in diabetic patients, and they are associated with a significant economic burden for healthcare systems. Vascular leakage is one of the earlier hallmarks in diabetic microvascular complications. Ezrin, radixin and moesin (ERM) proteins have recently been involved in vascular dysfunction under the effect of molecular mediators of diabetes complications. In this review we will present the available evidence regarding the role of these proteins in vascular leakage and their putative implication in diabetic microvascular complications...
October 16, 2018: Current Medicinal Chemistry
Sunkuk Kwon, Fred Christian Velasquez, Eva M Sevick-Muraca
In this study we employ a near-infrared fluorescence lymphatic imaging (NIRFLI) technique to longitudinally image spatial and temporal changes in the lymphatics in mice bearing vascular endothelial growth factor (VEGF)-C overexpressing B16F10 (VEGF-C-B16F10) or mock-transduced B16F10 (mock-B16F10) melanoma tumors. Our NIRFLI data show that ICG-laden lymph accumulates into a VEGF-C-B16F10 tumor compared to mock-B16F10 at 3 days post implantation, presumably due to increased lymphatic vessel permeability. Quantification shows a significantly greater percentage of ICG-perfused area in VEGF-C-B16F10 (7...
October 1, 2018: Biomedical Optics Express
Xiaofei An, Maoxiang Zhang, Sisi Zhou, Tian Lu, Yongjun Chen, Lin Yao
Hyperglycemia induces glomerular hyper-filtration, which contributes to the development of diabetic nephropathy (DN), a condition that remains a challenge for treatment. The present study investigated the effect of Xiao-Shen-Formula (XSF) used for treatment of renal injury in type 1 DN mice model induced by streptozotocin (STZ) and its underlying mechanism in cultured human glomerular endothelial cell (hGECs). Studies were performed using control, diabetic DN, DN treated with XSF groups (1 g/kg/d, LXSF or 3 g/kg/d, HXSF) for 6 weeks and hGECs were post-treated with mice serum containing HXSF (MS-HXSF) and arginase inhibitor (ABH, 100 μM) in high glucose medium...
2018: Frontiers in Physiology
Asmah Ahmad, Bariah Mohd-Ali, Bashirah Ishak
Purpose: Soft contact lens wear is most frequently associated with morphologic changes in corneal endothelial cells, but the data are scarce in Asians. This study aimed to evaluate changes in corneal endothelial cell morphology after 6 months of wearing soft hydrogel contact lenses (SH) in young myopic adults living in Kuala Lumpur. Patients and methods: Forty-eight subjects were included in the study, 24 were fitted with SH contact lenses (A) and the rest were prescribed with glasses (B)...
2018: Clinical optometry
Behnaz Aghaei-Ghareh-Bolagh, Juan Guan, Yiwei Wang, Adam D Martin, Rebecca Dawson, Suzanne M Mithieux, Anthony S Weiss
Damaged corneas can lead to blindness. Due to the worldwide shortage of donor corneas there is a tremendous unmet demand for a robust corneal replacement that supports growth of the major corneal cell types. Commercial artificial corneas comprise plastic polymers that do not adequately support diverse cell growth. We present a new class of protein elastomer-dominated synthetic corneas with attractive performance that intimately couple biologically active tropoelastin to mechanically robust and durable protein silk...
October 8, 2018: Biomaterials
Helen Triantafyllidi, Dimitris Benas, Stefanos Vlachos, Dimitris Vlastos, George Pavlidis, Antonios Schoinas, Mary Varoudi, Dionysia Birmpa, Paraskevi Moutsatsou, John Lekakis, Ignatios Ikonomidis
Endothelial dysfunction indicates target organ damage in hypertensive patients. The integrity of endothelial glycocalyx (EG) plays a vital role in vascular permeability, inflammation and elasticity, and finally to cardiovascular disease. The authors aimed to investigate the role of increased HDL cholesterol (HDL-C) levels, which usually are considered protective against cardiovascular disease, in EG integrity in older hypertensive patients. The authors studied 120 treated hypertensive patients older than 50 years were divided regarding HDL-C tertiles in group HDLH (HDL-C ≥ 71 mg/dL, upper HDL-C tertile) and group HDLL (HDL-C < 71 mg/dL, two lower HDL-C tertiles)...
October 13, 2018: Journal of Clinical Hypertension
Bingmei M Fu
Tumor cell metastasis through blood circulation is a complex process and is one of the great challenges in cancer research as metastatic spread is responsible for ∼90% of cancer-related mortality. Tumor cell intravasation into, arrest and adhesion at, and extravasation from the microvessel walls are critical steps in metastatic spread. Understanding these steps may lead to new therapeutic concepts for tumor metastasis. Vascular endothelium forming the microvessel wall and the glycocalyx layer at its surface are the principal barriers to and regulators of the material exchange between circulating blood and body tissues...
2018: Advances in Experimental Medicine and Biology
Fitz-Roy E Curry
There has been rapid progress over the past decade to extend the concept that a quasiperiodic inner endothelial glycocalyx layer (EGL, <300 nm thick, with key components associated with the endothelial cell membrane) forms the primary molecular filter between circulating blood and the body tissues. The EGL is common to both continuous and fenestrated microvessels. The revised Starling Principle describing steady-state fluid exchange across the EGL describes new ways to understand transvascular exchange of water and plasma proteins in microvessels in both normal and disturbed states such as hemorrhage and fluid replacement during surgery...
2018: Advances in Experimental Medicine and Biology
Lina Huang, Xiaohua Zhang, Xiaohong Ma, Dong Zhang, Dongxiao Li, Jiali Feng, Xinjie Pan, Junhong Lü, Xiaozhi Wang, Xiangyong Liu
In the pathogenesis of acute respiratory distress syndrome (ARDS), an increase in vascular endothelial permeability may trigger pulmonary edema and ultimately lead to respiratory failure. Endothelial glycocalyx damage is an important factor that causes an increase in vascular endothelial permeability. Berberine (BBR) is an isoquinoline alkaloid extracted from Coptis chinensis, a plant used in traditional Chinese medicine that exerts multiple pharmacological effects. In this study, pretreatment with BBR inhibited the increase in vascular endothelial permeability in mice with lipopolysaccharide (LPS)-induced ARDS...
October 8, 2018: International Immunopharmacology
Rayana A P Maciel, Regiane S Cunha, Valentina Busato, Célia R C Franco, Paulo C Gregório, Carla J R Dolenga, Lia S Nakao, Ziad A Massy, Agnès Boullier, Roberto Pecoits-Filho, Andréa E M Stinghen
Endothelial dysfunction in uremia can result in cell-to-cell junction loss and increased permeability, contributing to cardiovascular diseases (CVD) development. This study evaluated the impact of the uremic milieu on endothelial morphology and cell junction's proteins. We evaluated (i) serum levels of inflammatory biomarkers in a cohort of chronic kidney disease (CKD) patients and the expression of VE-cadherin and Zonula Occludens-1 (ZO-1) junction proteins on endothelial cells (ECs) of arteries removed from CKD patients during renal transplant; (ii) ECs morphology in vitro under different uremic conditions, and (iii) the impact of uremic toxins p-cresyl sulfate (PCS), indoxyl sulfate (IS), and inorganic phosphate (Pi) as well as of total uremic serum on VE-cadherin and ZO-1 gene and protein expression in cultured ECs...
October 7, 2018: Toxins
Chih-Yao Chiang, Chen-Yen Chien, Wei-Yin Qiou, Christopher Chang, I-Shing Yu, Po-Yuan Chang, Chiang-Ting Chien
OBJECTIVE:  Activation of thromboxane A2 synthase (TXAS)/thromboxane A2 (TXA2 )/thromboxane prostanoid (TP) receptor leads to arterial constriction, platelet aggregation and vascular injury. We attempted to characterize the microvascular dysfunction in ischaemia/reperfusion injury using genetically modified TXAS-/- , TP-/- and TXAS-/- TP-/- mice. APPROACH AND RESULTS:  The cardiac micro-circulation and electrocardiograms were evaluated from B6, TXAS-/- , TP-/- and TXAS-/- TP-/- mice in response to intravenous saline, endothelin-1, U46619 (a TXA2 agonist) and myocardial ischaemia/reperfusion injury...
October 9, 2018: Thrombosis and Haemostasis
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