keyword
https://read.qxmd.com/read/38484189/a-temporal-developmental-map-separates-human-nk-cells-from-non-cytotoxic-ilcs-through-clonal-and-single-cell-analysis
#1
JOURNAL ARTICLE
Dang Nghiem Vo, Ouyang Yuan, Minoru Kanaya, Gladys Telliam-Dushime, Hongzhe Li, Olga Kotova, Emel Caglar, Kristian Honnens de Lichtenberg, Shamim Herbert Rahman, Shamit Soneji, Stefan Scheding, David Bryder, Karl-Johan Malmberg, Ewa Sitnicka
Natural Killer (NK) cells represent the cytotoxic member within the innate lymphoid cell (ILC) family that are important against viral infections and cancer. While the NK cell emergence from hematopoietic stem and progenitor cells through multiple intermediate stages and the underlying regulatory gene network has been extensively studied in mouse, this process is not well characterized in human. Here, using a temporal in vitro model to reconstruct the developmental trajectory of NK lineage, we identified an ILC-restricted oligo-potent Stage 3a CD34-CD117+CD161+CD45RA+CD56- progenitor population, that exclusively gave rise to CD56-expressing ILCs in vitro...
March 14, 2024: Blood Advances
https://read.qxmd.com/read/38481242/uncovering-the-cellular-and-omics-characteristics-of-natural-killer-cells-in-the-bone-marrow-microenvironment-of-patients-with-acute-myeloid-leukemia
#2
JOURNAL ARTICLE
Leisheng Zhang, Yunyan Sun, Chun-E Xue, Shuling Wang, Xianghong Xu, Chengyun Zheng, Cunrong Chen, Dexiao Kong
BACKGROUND: Acute myeloid leukemia (AML) is a highly heterogeneous hematologic malignancy and the most frequently acute leukemia of stem cell precursors and the myeloid derivatives in adult. Longitudinal studies have indicated the therapeutic landscape and drug resistance for patients with AML are still intractable, which largely attribute to the deficiency of detailed information upon the pathogenesis. METHODS: In this study, we compared the cellular phenotype of resident NK cells (rAML-NKs, rHD-NKs) and expanded NK cells (eAML-NKs, eHD-NKs) from bone marrow of AML patients (AML) and healthy donors (HD)...
March 14, 2024: Cancer Cell International
https://read.qxmd.com/read/38412933/angelica-gigas-polysaccharide-induces-cr3-mediated-macrophage-activation-and-the-cytotoxicity-of-natural-killer-cells-against-hct-116-cells-via-nf-%C3%AE%C2%BAb-and-mapk-signaling-pathways
#3
JOURNAL ARTICLE
Yunfei Ge, Subramanian Palanisamy, Mi-Hye Kwon, Fang Kou, Rajavel Arumugam Uthamapriya, Lee Dong Jin, Duyun Jeong, Honghui Bao, SangGuan You
Angelica gigas (A. gigas) is traditional medicinal herb that mainly exists in Korea and northeastern China. There have been relatively few studies conducted thus far on its polysaccharides and their bioactivities. We purified and described a novel water-soluble polysaccharide derived from A. gigas and investigated its immunoenhancing properties. The basic components of crude and purified polysaccharides (F1 and F2) were total sugar (41.07 % - 70.55 %), protein (1.12-10.33 %), sulfate (2...
February 25, 2024: International Journal of Biological Macromolecules
https://read.qxmd.com/read/38396930/involvement-of-m1-activated-macrophages-and-perforin-granulysin-expressing-lymphocytes-in-iga-vasculitis-nephritis
#4
JOURNAL ARTICLE
Gordana Laskarin, Emina Babarovic, Nastasia Kifer, Stela Bulimbasic, Mario Sestan, Martina Held, Marijan Frkovic, Alenka Gagro, Marijana Coric, Marija Jelusic
We investigated the polarisation of CD68+ macrophages and perforin and granulysin distributions in kidney lymphocyte subsets of children with IgA vasculitis nephritis (IgAVN). Pro-inflammatory macrophage (M)1 (CD68/iNOS) or regulatory M2 (CD68/arginase-1) polarisation; spatial arrangement of macrophages and lymphocytes; and perforin and granulysin distribution in CD3+ and CD56+ cells were visulaised using double-labelled immunofluorescence. In contrast to the tubules, iNOS+ cells were more abundant than the arginase-1+ cells in the glomeruli...
February 13, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38363249/source-of-hematopoietic-progenitor-cells-determines-their-capacity-to-generate-innate-lymphoid-cells-ex-vivo
#5
JOURNAL ARTICLE
Said Z Omar, Vera van Hoeven, Nienke J E Haverkate, Jolien M R Van der Meer, Carlijn Voermans, Bianca Blom, Mette D Hazenberg
BACKGROUND AIMS: The success of allogeneic hematopoietic cell transplantation (HCT) as therapy for hematologic conditions is negatively impacted by the occurrence of graft-versus-host disease (GVHD). Tissue damage, caused, for example, by chemotherapy and radiotherapy, is a key factor in GVHD pathogenesis. Innate lymphoid cells (ILCs) are important mediators of tissue repair and homeostasis. The presence of ILCs before, and enhanced ILC reconstitution after, allogeneic HCT is associated with a reduced risk to develop mucositis and GVHD...
February 14, 2024: Cytotherapy
https://read.qxmd.com/read/38326202/donor-age-correlates-with-liver-resident-natural-killer-cell-activity-in-adoptive-immunotherapy-using-donor-liver-natural-killer-cells-in-liver-transplantation
#6
JOURNAL ARTICLE
Masahiro Ohira, Kouki Imaoka, Tomoaki Bekki, Koki Sato, Yuki Imaoka, Ryosuke Nakano, Takuya Yano, Marlen Doskali, Seiichi Shimizu, Ichiya Chogahara, Saki Sato, Mayuna Nakamura, Yuka Tanaka, Hideki Ohdan
BACKGROUND: Natural killer (NK) cells are involved in innate immunity and have been reported to play an important role in hepatocellular carcinoma recurrence and post-liver transplantation (LT) infection. However, the relationship between donor age and liver-resident NK cell activity remains to be elucidated. METHODS: We successfully performed NK cell immunotherapy in 19 living donor LT recipients to prevent post-LT bloodstream infections. Liver mononuclear cells (LMNCs) were collected from the liver graft perfusate and stimulated with interleukin 2 for 3 days...
February 6, 2024: Transplantation Proceedings
https://read.qxmd.com/read/38251887/a-noncanonical-cd56dimcd16dim-nk-cell-subset-indicative-of-prior-cytotoxic-activity-is-elevated-in-patients-with-autoantibody-mediated-neurologic-diseases
#7
JOURNAL ARTICLE
Soumya S Yandamuri, Beata Filipek, Nikhil Lele, Inessa Cohen, Jeffrey L Bennett, Richard J Nowak, Elias S Sotirchos, Erin E Longbrake, Emily M Mace, Kevin C O'Connor
Neuromyelitis optica spectrum disorder (NMOSD), myelin oligodendrocyte glycoprotein Ab disease, and autoimmune myasthenia gravis (MG) are autoantibody-mediated neurologic conditions where autoantibodies can induce Ab-dependent cellular cytotoxicity (ADCC), a NK cell-mediated effector function. However, whether ADCC is a pathogenic mechanism in patients with these conditions has not been confirmed. We sought to characterize circulatory NK cells using functional assays, phenotyping, and transcriptomics to elucidate their role in pathology...
March 1, 2024: Journal of Immunology
https://read.qxmd.com/read/38240638/chimeric-antigen-cytotoxic-receptors-for-in-vivo-engineering-of-tumor-targeting-nk-cells
#8
JOURNAL ARTICLE
Neha Diwanji, Daniel Getts, Yuxiao Wang
Chimeric Ag receptor (CAR) NK cells are challenging to manufacture and fail to achieve consistent tumor infiltration and sustained cytolytic function in the tumor microenvironment. In vivo engineering of NK cells using mRNA-based CAR delivery may overcome these issues. In this study, we developed an in vivo programming method by designing CARs that leverage the biology of NK cell receptors for cell type-specific expression and function. These CARs were engineered by fusion of a tumor recognition domain with the natural cytotoxic receptor family including NKp30, NKp44, and NKp46...
January 1, 2024: ImmunoHorizons
https://read.qxmd.com/read/38117750/cutting-edge-pdgf-dd-binding-to-nkp44-costimulates-tlr9-signaling-and-proinflammatory-cytokine-secretion-in-human-plasmacytoid-dendritic-cells
#9
JOURNAL ARTICLE
Alexander David Barrow, Marina Cella, Melissa Anne Edeling, Md Abdullah-Al-Kamran Khan, Luisa Cervantes-Barragan, Mattia Bugatti, Christian Schmedt, William Vermi, Marco Colonna
NKp44 is a human receptor originally found on activated NK cells, group 1 and group 3 innate lymphoid cells that binds dimers of platelet-derived growth factor D (PDGF-DD). NKp44 is also expressed on tissue plasmacytoid dendritic cells (PDCs), but NKp44-PDGF-DD interaction on PDCs remains unstudied. Engagement of NKp44 with PDGF-DD in vitro enhanced PDC secretion of IFN-α, TNF, and IL-6 in response to the TLR9 ligand CpG-ODN, but not TLR7/8 ligands. In tissues, PDCs were found in close contact with PDGF-DD-expressing cells in the high endothelial venules and epithelium of tonsils, melanomas, and skin lesions infected with Molluscum contagiosum...
December 20, 2023: Journal of Immunology
https://read.qxmd.com/read/37957274/an-immunomodulating-peptide-with-potential-to-suppress-tumour-growth-and-autoimmunity
#10
JOURNAL ARTICLE
Michael Agrez, Christopher Chandler, Kristofer J Thurecht, Nicholas L Fletcher, Feifei Liu, Gayathri Subramaniam, Christopher B Howard, Benjamin Blyth, Stephen Parker, Darryl Turner, Justyna Rzepecka, Gavin Knox, Anastasia Nika, Andrew M Hall, Hayley Gooding, Laura Gallagher
Cancers and autoimmune diseases commonly co-exist and immune checkpoint inhibitor therapy (ICI) exacerbates autoimmune pathologies. We recently described a lipidic peptide, designated IK14004, that promotes expansion of immunosuppressive T regulatory (Treg) cells and uncouples interleukin-2 from interferon-gamma production while activating CD8+ T cells. Herein, we report IK14004-mediated inhibition of Lewis lung cancer (LLC) growth and re-invigoration of splenocyte-derived exhausted CD4+ T cells...
November 13, 2023: Scientific Reports
https://read.qxmd.com/read/37933909/wtap-enhances-the-instability-of-sytl1-mrna-caused-by-ythdf2-in-bladder-cancer
#11
JOURNAL ARTICLE
Jiansong Wang, Jianjun Luo, Xuecheng Wu, Zhuo Li
BACKGROUND: Bladder cancer (BCa) is the most frequent type of cancer in humans. The association between m6A modification and the anti-tumor effects of natural killer (NK) cells has been described in BCa. This study intended to investigate the implications of m6A regulators in modulating SYTL1 expression in BCa and the association with the anti-tumor effects of NK cells. METHODS: The prognostic role of SYTL1 in BCa was investigated using bioinformatics analysis, and the correlation between SYTL1 expression and NK cells was analyzed...
October 23, 2023: Histology and Histopathology
https://read.qxmd.com/read/37908353/enhanced-expression-of-natural-cytotoxicity-receptors-on-cytokine-induced-memory-like-natural-killer-cells-correlates-with-effector-function
#12
JOURNAL ARTICLE
Sofía Carreira-Santos, Nelson López-Sejas, Marina González-Sánchez, Eva Sánchez-Hernández, Alejandra Pera, Fakhri Hassouneh, Esther Durán, Rafael Solana, Javier G Casado, Raquel Tarazona
INTRODUCTION: Natural killer (NK) cells are a key component of the innate immune system, involved in defending the host against virus-infected cells and tumor immunosurveillance. Under in vitro culture conditions, IL-12/15/18 can induce a memory-like phenotype in NK cells. These cytokine-induced memory-like (CIML) NK cells possess desirable characteristics for immunotherapies, including a longer lifespan and increased cytotoxicity. METHODS: In this study, NK cells were isolated from peripheral blood of healthy donors and stimulated with IL-12/15/18 to induce a memory-like phenotype or with IL-15 alone as a control...
2023: Frontiers in Immunology
https://read.qxmd.com/read/37887757/natural-killer-repertoire-restoration-in-tb-hiv-co-infected-individuals-experienced-an-immune-reconstitution-syndrome-camelia-trial-anrs-12153
#13
JOURNAL ARTICLE
Polidy Pean, Yoann Madec, Eric Nerrienet, Laurence Borand, Didier Laureillard, Marcelo Fernandez, Olivier Marcy, Daniel Scott-Algara
IRIS is a common complication in HIV-infected patients treated for tuberculosis (TB) and cART. Our aim was to evaluate NK cell reconstitution in HIV-infected patients with TB-IRIS compared to those without IRIS. 147 HIV-infected patients with TB from the CAMELIA trial were enrolled. HIV+TB+ patients were followed for 32 weeks. The NK cell repertoire was assessed in whole blood at different time points. As CAMELIA has two arms (early and late cART initiation), we analysed them separately. At enrolment, individuals had low CD4 cell counts (27 cells/mm3 ) and high plasma viral loads (5...
October 13, 2023: Pathogens
https://read.qxmd.com/read/37834182/functional-trpa1-channels-regulate-cd56-dim-cd16-nk-cell-cytotoxicity-against-tumor-cells
#14
JOURNAL ARTICLE
Fernanda Scopelliti, Valentina Dimartino, Caterina Cattani, Andrea Cavani
Transient receptor potential ankyrin 1 (TRPA1) channels are expressed on the surface of different cell types, including immune cells. However, TRPA1's role in the context of innate and adaptive immune responses has not been fully elucidated so far. In this study, we aimed at investigating the expression and function of TRPA1 channels on NK cells. Among NK cells, TRPA1 was highly expressed by the CD56dim CD16+ subpopulation, but not by CD56bright CD16- cells, as detected by FACS. TRPA1 activation with the potent ligand allyl isothiocyanate (AITC) induces intracellular calcium flux in CD56dim CD16+ cells, which was prevented by the TRPA1 antagonist HC-030031...
September 29, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37788895/-hla-dpa1-02-01-b1-01-01-is-a-risk-haplotype-for-primary-sclerosing-cholangitis-mediating-activation-of-nkp44-nk-cells
#15
JOURNAL ARTICLE
Britta F Zecher, David Ellinghaus, Sebastian Schloer, Annika Niehrs, Benedetta Padoan, Martin E Baumdick, Yuko Yuki, Maureen P Martin, Dawid Glow, Jennifer Schröder-Schwarz, Jennifer Niersch, Sébastien Brias, Luisa M Müller, Robin Habermann, Paul Kretschmer, Tristan Früh, Janis Dänekas, Malte H Wehmeyer, Tobias Poch, Marcial Sebode, Eva Ellinghaus, Frauke Degenhardt, Christian Körner, Angelique Hoelzemer, Boris Fehse, Karl J Oldhafer, Udo Schumacher, Guido Sauter, Mary Carrington, Andre Franke, Madeleine J Bunders, Christoph Schramm, Marcus Altfeld
OBJECTIVE: Primary sclerosing cholangitis (PSC) is characterised by bile duct strictures and progressive liver disease, eventually requiring liver transplantation. Although the pathogenesis of PSC remains incompletely understood, strong associations with HLA-class II haplotypes have been described. As specific HLA-DP molecules can bind the activating NK-cell receptor NKp44, we investigated the role of HLA-DP/NKp44-interactions in PSC. DESIGN: Liver tissue, intrahepatic and peripheral blood lymphocytes of individuals with PSC and control individuals were characterised using flow cytometry, immunohistochemical and immunofluorescence analyses...
January 5, 2024: Gut
https://read.qxmd.com/read/37686697/mab14-a-monoclonal-antibody-against-cell-surface-pcna-a-potential-tool-for-sezary-syndrome-diagnosis-and-targeted-immunotherapy
#16
JOURNAL ARTICLE
Jamal Knaneh, Emmilia Hodak, Shlomit Fedida-Metula, Avishay Edri, Rachel Eren, Yael Yoffe, Iris Amitay-Laish, Hadas Prag Naveh, Ido Lubin, Angel Porgador, Lilach Moyal
Mycosis fungoides (MF) and Sézary syndrome (SS) are the most common types of primary cutaneous T-cell lymphoma (CTCL). Proliferating cell nuclear antigen (PCNA) is expressed on the cell surface of cancer cells (csPCNA), but not on normal cells. It functions as an immune checkpoint ligand by interacting with natural killer (NK) cells through the NK inhibitory receptor NKp44, leading to the inhibition of NK cytotoxicity. A monoclonal antibody (mAb14) was established to detect csPCNA on cancer cells and block their interaction with NKp44...
September 4, 2023: Cancers
https://read.qxmd.com/read/37606864/a-concise-chemoenzymatic-total-synthesis-of-neutral-globo-series-glycosphingolipids-globo-a-and-globo-b-and-forssman-and-para-forssman-antigens
#17
JOURNAL ARTICLE
Yu-Ching Chiang, Chun-Yen Wu, Pei-Yun Chiang, Avijit K Adak, Chun-Cheng Lin
Globo A is a neutral Globo-series glycosphingolipid (GSL) that shows natural properties of a cytotoxicity receptor NKp44 binding ligand. The highly complex heptasaccharide glycan structure of Globo A combined with its biological profile provides a unique target for the development of a synthetic method to facilitate its bioactivity studies. Here, a concise chemoenzymatic route to the synthesis of Globo A and its α1,3-galactose-linked congener Globo B is reported. The key to success was the use of a synthetic azido β-Globo H sphingosine (Globo H-βSph) as an acceptor substrate and two glycosyl transferases, an α1,3-N-acetylgalactosaminyltransferase from Helicobacter mustelae (BgtA) and a human blood group B α1,3-galactosyltransferase (h1,3GTB), for stereoselective construction of the terminal α1,3-GalNAc and α1,3-Gal linkages, respectively...
August 22, 2023: Glycoconjugate Journal
https://read.qxmd.com/read/37501379/role-of-interleukin-il-6-in-nk-activity-to-hypoxic-induced-highly-invasive-hepatocellular-carcinoma-hcc-cells
#18
JOURNAL ARTICLE
Hwan Hee Lee, Hyojung Kang, Hyosun Cho
Natural killer (NK) cell dysfunctions against hepatocellular carcinoma (HCC) in a hypoxic environment. Many solid tumors are present in a hypoxic condition, which changes the effector function of various immune cells. The transcription of hypoxic-inducible factors (HIFs) in cancer cells make it possible to adapt to their hypoxic environment and to escape the immune surveillance of NK cells. Recently, the correlation between the transcription of HIF-1α and pro-inflammatory cytokines has been reported. Interleukin (IL)-6 is higher in cancers with a highly invasive ability, and is closely related to the metastasis of cancers...
July 28, 2023: Journal of Microbiology and Biotechnology
https://read.qxmd.com/read/37454979/hla-dp-on-epithelial-cells-enables-tissue-damage-by-nkp44-nk-cells-in-ulcerative-colitis
#19
JOURNAL ARTICLE
Martin E Baumdick, Annika Niehrs, Frauke Degenhardt, Maria Schwerk, Ole Hinrichs, Ana Jordan-Paiz, Benedetta Padoan, Lucy H M Wegner, Sebastian Schloer, Britta F Zecher, Jakob Malsy, Vinita R Joshi, Christin Illig, Jennifer Schröder-Schwarz, Kimberly J Möller, Maureen Martin, Yuko Yuki, Mikki Ozawa, Jürgen Sauter, Alexander H Schmidt, Daniel Perez, Anastasios D Giannou, Mary Carrington, Randall S Davis, Udo Schumacher, Guido Sauter, Samuel Huber, Victor G Puelles, Nathaniel Melling, Andre Franke, Marcus Altfeld, Madeleine J Bunders
BACKGROUND AND AIMS: Ulcerative colitis (UC) is characterized by severe inflammation and destruction of the intestinal epithelium, and associated with risk single nucleotide polymorphism in HLA class II. Given the recently discovered interactions between subsets of HLA-DP molecules and the activating natural killer (NK) cell receptor NKp44, genetic associations of UC and HLA-DP haplotypes and their functional implications were investigated. METHODS: HLA-DP haplotype and UC risk association analyses were performed (UC: N= 13,927; control: N= 26,764)...
July 14, 2023: Gastroenterology
https://read.qxmd.com/read/37426355/early-trail-engagement-elicits-potent-multimodal-targeting-of-melanoma-by-cd34-progenitor-cell-derived-nk-cells
#20
JOURNAL ARTICLE
Amanda A van Vliet, Ella Peters, Denise Vodegel, Daniëlle Steenmans, Monica Raimo, Susan Gibbs, Tanja D de Gruijl, Adil D Duru, Jan Spanholtz, Anna-Maria Georgoudaki
Umbilical cord blood (UCB) CD34+ progenitor cell-derived natural killer (NK) cells exert efficient cytotoxicity against various melanoma cell lines. Of interest, the relative cytotoxic performance of individual UCB donors was consistent throughout the melanoma panel and correlated with IFNγ, TNF, perforin and granzyme B levels. Importantly, intrinsic perforin and Granzyme B load predicts NK cell cytotoxic capacity. Exploring the mode of action revealed involvement of the activating receptors NKG2D, DNAM-1, NKp30, NKp44, NKp46 and most importantly of TRAIL...
July 21, 2023: IScience
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