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2D Analysis

Xiong Deng, Shoumin Zhen, Dongmiao Liu, Yue Liu, Mengfei Li, Nannan Liu, Yueming Yan
Integrated proteome analyses revealed differentially accumulated proteins in the non-leaf green organs in wheat glume and awn that play important roles in photosynthesis and drought resistance. Two non-leaf green organs in wheat, glume and awn, have photosynthetic potential, contribute to grain yield, and also play roles in resistance to adverse conditions. We performed the first integrated proteome analysis of wheat glume and awn in response to water deficit. Water deficit caused a significant decrease in important agronomic traits and grain yield...
November 16, 2018: Journal of Plant Physiology
Ashley A Roark, Pragya A Dang, Bethany L Niell, Elkan F Halpern, Constance D Lehman
OBJECTIVE: The objective of our study was to compare the supplemental cancer yield and performance of breast MRI in women at higher-than-average risk for breast cancer after negative 2D full-field digital mammography (FFDM) or negative digital breast tomosynthesis (DBT). MATERIALS AND METHODS: Retrospective review identified 4418 screening breast MRI examinations: 2291 were performed from January 2010 through January 2012 of patients with a negative FFDM examination in the 12 months before MRI (FFDM group), and 2127 were performed from January 2013 through January 2015 of patients with a negative DBT examination in the 12 months before MRI (DBT group)...
December 12, 2018: AJR. American Journal of Roentgenology
Paul Simkin, Anand Rattansingh, Kuan Liu, John M Hudson, Mostafa Atri, Hyun-Jung Jang, Tae Kyoung Kim, Korosh Khalili
OBJECTIVES: The purpose of this study was to compare the reliability and agreement of 2 methods of 2-dimensional (2D) shear wave elastography (SWE) on liver stiffness in healthy volunteers. We also assessed effects of the prandial state and operator experience on measurements. METHODS: Two operators, 1 experienced and 1 novice, independently examined 20 healthy volunteers with 2D SWE on 2 ultrasound machines (Aixplorer [SuperSonic Imagine, Aix-en-Provence, France] and Aplio 500 [Canon Medical Systems Corporation, Otawara, Japan])...
December 11, 2018: Journal of Ultrasound in Medicine: Official Journal of the American Institute of Ultrasound in Medicine
Kaipu Jin, Heqing Wang, Mengsu Zeng, Shengxiang Rao, Lixia Yan, Yuan Ji, Caixia Fu, Ruofan Sheng
OBJECTIVE: To evaluate the value of MR liver extracellular volume (ECVliver ) in assessment of liver fibrosis with chronic hepatitis B (CHB), and to compare its performance with two-dimensional (2D) shear-wave elastography (SWE). MATERIALS AND METHODS: A total of 68 CHB patients who were histologically diagnosed as fibrosis stages F0 to F4 were retrospectively analyzed. All patients underwent gadopentetate dimeglumine-enhanced T1-mapping and 2D SWE. ECVliver and liver stiffness were measured and compared between fibrosis subgroups; their correlations with histologic findings were evaluated using Spearman correlation test and multiple regression analysis...
December 7, 2018: Abdominal Radiology
Hans Rabus, Sofia Barbieri, Giorgio Baiocco, Andrea Ottolenghi, Ulrich Giesen
When early radiation damage to biological systems is studied based on the formation of foci at the location of DNA double-strand breaks, the foci observed in irradiated cells either may be induced by ionizing radiation (IR) interactions or they may be due to other causes that lead to observation of foci also in unirradiated cells. Generally, to take account of the latter, additional samples are taken where the exposure to IR is skipped in the protocol. The data analysis relies on statistical independence of the frequency distributions of background and radiation-induced foci...
December 11, 2018: Radiation Protection Dosimetry
Jianhui Yang, Anping Wang, Shaozheng Zhang, Jia Liu, Zhicheng Zhong, Liang Chen
Akin to three dimensional (3D) multiferroics, two dimensional (2D) piezoelectric materials with intrinsic magnetic properties are promising applications in nanoscale spintronic devices. In this study, 2D magnetic transition metal dichalcogenides (VS2, VSe2, and Janus-VSSe) have been investigated by the first principles method for their structural, magnetic, electronic, and piezoelectric properties. H type Janus-VSSe has been shown to be more stable than the T type, and dynamically stable through phonon frequency analysis...
December 10, 2018: Physical Chemistry Chemical Physics: PCCP
Jonathan C J Wei, Isha N Haridass, Michael L Crichton, Yousuf H Mohammed, Stefano C Meliga, Washington Y Sanchez, Jeffrey E Grice, Heather A E Benson, Michael S Roberts, Mark A F Kendall
Microscale medical devices are being developed for targeted skin delivery of vaccines and the extraction of biomarkers, with the potential to revolutionise healthcare in both developing and developed countries. The effective clinical development of these devices is dependent on understanding the macro-molecular diffusion properties of skin. We hypothesised that diffusion varied according to specific skin layers. Using three different molecular weights of rhodamine dextran (RD) (MW of 70, 500 and 2000 kDa) relevant to the vaccine and therapeutic scales, we deposited molecules to a range of depths (0-300 µm) in ex vivo human skin using the Nanopatch device...
December 10, 2018: Scientific Reports
Elaine O R Viana, Maria de Fátima S J Cruz, Marília J da Silva, Gabriela M Pereira, Bernadete P da Silva, Luzineide W Tinoco, José P Parente
As part of the ongoing efforts in discovering potentially bioactive natural products from medicinal plants, the present study was conducted to isolate a new complex triterpenoid saponin from the barks of Albizia lebbeck. It was isolated by using chromatographic methods and its structural elucidation was performed using detailed analyses of 1 H and 13 C NMR spectra including 2D-NMR (COSY, TOCSY, HSQC and HMBC) spectroscopic techniques, high-resolution electrospray ionization mass spectrometry (HRESIMS) analysis and chemical conversions...
November 27, 2018: Carbohydrate Research
Nanyoung Kim, SeonJu Park, Nguyen Xuan Nhiem, Jae-Hyoung Song, Hyun-Jeong Ko, Seung Hyun Kim
Five undescribed cycloartane-type triterpenoids, which were isolated for the first time from the genus, and a flavonoid glycoside together with 11 known compounds were isolated from the burs of Castanea crenata. The structures were elucidated based on the spectroscopic analysis of 1D and 2D NMR and MS data. All isolated compounds were evaluated for antiviral activities against HRV1B-, CVB3-, and PR8-infected cells. Most kaempferol derivatives showed statistically significant antiviral activities against HRV1B-infected cells...
December 6, 2018: Phytochemistry
Fahimeh Varzideh, Sara Pahlavan, Hassan Ansari, Majid Halvaei, Sawa Kostin, Mohammad-Sadegh Feiz, Hamid Latifi, Nasser Aghdami, Thomas Braun, Hossein Baharvand
Human cardiomyocytes (CM) differentiated from pluripotent stem cells (PSCs) are relatively immature when generated in two-dimensional (2D) in vitro cultures, which limits their biomedical applications. Here, we devised a strategy to enhance maturation of human CM in vitro by assembly of three-dimensional (3D) cardiac organoids (CO) containing human embryonic stem cell-derived cardiac progenitor cells (hESC-CPCs), endothelial cells (ECs), and mesenchymal stem cells (MSCs). In contrast to corresponding 2D cultures, 3D CO not only developed into structures containing spontaneously beating CM, but also showed enhanced maturity as indicated by increased expressions of sarcomere and ion channel genes and reduced proliferation...
November 27, 2018: Biomaterials
Jemmyson Romário de Jesus, Ivanilce Cristina Guimarães, Marco Aurélio Zezzi Arruda
A combination between gel electrophoresis and smartphone technology is applied for quantifying proteins in a serum sample. Electrophoresis not only allows one to separate different proteins but also to build-up a calibration curve for those proteins. To expand its applicability, a smartphone allows one to capture a gel image, through its camera. Additionally, the treatment of the data extracted through each protein band is performed using a freely available program (PhotoMetrix), which is downloaded to one's own smartphone...
December 6, 2018: Journal of Proteomics
Katarina M Rajković, Nebojša T Milošević, Suzana Otašević, Sanja Jeremić, Valentina Arsić Arsenijević
BACKGROUND: Aspergillus fumigatus causes serious infections in humans, and its virulence correlates with hyphal growth, branching and formation of the filamentous mycelium. The filamentous mycelium is a complex structure inconvenient for quantity analysis. In this study, we monitored the branching of A. fumigatus filamentous mycelium in vitro at different points in time in order to assess the complexity degree and develop a dynamic model for the branching complexity. METHOD: We used fractal analysis of microscopic images (FAMI) to measure the fractal dimensions (D) of the branching complexity within 24 h of incubation...
November 28, 2018: Computers in Biology and Medicine
Rachel Henley, Vidya Chandrasekaran, Cecilia Giulivi
Dendrites are the primary site of synaptic activity in neurons and changes in synapses are often the first pathological stage in neurodegenerative diseases. Molecular studies of these changes rely on morphological analysis of the imaging of somas and dendritic arbors of cultured or primary neurons. As research on preventing or reversing synaptic degeneration develops, demands increase for user-friendly 2D neurite analyzers without undermining accuracy and reproducibility. The most common method of 2D neurite analysis is manual by using ImageJ...
December 6, 2018: Brain Research Bulletin
Bin Hu, Peifang Wang, Chao Wang, Jin Qian, Tianli Bao, Yue Shi
Different environmental dynamics of sediment organic matter (SOM) and suspended particulate organic matter (SPOM) result in great disparities in characteristics and subsequent interactions with heavy metals. In this study, sediments and suspended particles were collected from two large and shallow lakes (Taihu and Hongze Lake) to study the difference in the characteristics and copper binding properties between SOM and SPOM through spectroscopy method. Our results showed that SPOM in Taihu Lake was dominated by autochthonous tyrosine-like substance and SOM was dominated by terrestrial humic-like substance, whereas SPOM in Hongze Lake was dominated by terrestrial humic-like substances...
November 23, 2018: Chemosphere
Xin Jin, Weijie Zhang, Rui Hou, Pengkang Jin, Jina Song, Xiaochang C Wang
The characteristics of effluent organic matter (EfOM) from a wastewater treatment plant (WWTP) during ozonation were investigated using excitation and emission matrix (EEM) spectra, Fourier transform infrared spectroscopy (FT-IR) and high-performance size exclusion chromatography (HPSEC) at different ozone dosages. The selectivity of ozonation towards different constituents and functional groups was analysed using two-dimensional correlation spectra (2D-COS) probed by FT-IR, synchronous fluorescence spectra and HPSEC...
February 2019: Journal of Environmental Sciences (China)
Dan Wu, Ming Zhou, Liang Li, Xiangfeng Leng, Zheng Zhang, Ning Wang, Yanwei Sun
Severe burns might be followed by severe infection associated with high mortality. In this study, we aimed to identify changes in genes and processes across time points after burn via analyzing time series gene expression profiles in burn patients and control from the Gene Expression Omnibus (GEO). Patients were classified into four groups according to time after burn and weighted gene co-expression network analysis (WGCNA) obtained three gene modules including magenta, yellow and greenyellow modules that significantly correlated positively with time after burn...
December 6, 2018: Burns: Journal of the International Society for Burn Injuries
Jean-Nicolas Dumez
A family of high-resolution NMR methods share the common concept of acquiring in parallel different sub-experiments in different spatial regions of the NMR tube. These spatial encoding and spatial selection methods were for the most part introduced independently from each other and serve different purposes, but they share common ingredients, often derived from magnetic resonance imaging, and they all benefit from a greatly improved time-efficiency. This review article provides a description of several spatial encoding and spatial selection methods, including single-scan multidimensional experiments (ultrafast 2D NMR, DOSY, Z spectroscopy, inversion recovery and Laplace NMR), pure shift and selective refocusing experiments (including Zangger-Sterk decoupling, G-SERF and PSYCHE), a Z filter, and fast-pulsing slice-selective experiments...
December 2018: Progress in Nuclear Magnetic Resonance Spectroscopy
Kimberley Jacobs, Diego Riveros, Heather K Vincent, Daniel C Herman
Different landing surfaces may affect lower extremity biomechanical performance during athletic tasks. The magnitude of this effect on clinical screening measures such as jump-landings is unknown. This study determined the effect of court (CS), grass (GS), and tile (TS) surfaces on Landing Error Scoring System (LESS) grades. A repeated-measures design was used. A total of 40 (21F, 19M; mean age = 23.8 ± 2.4 yr) recreational athletes performed a jump-landing task on three different landing surfaces. 2D videography recorded jump-landings in the frontal and sagittal planes...
December 10, 2018: Sports Biomechanics
Rumit Singh Kakar, Hilary B Greenberger, Patrick O McKeon
CONTEXT: Anterior knee pain also known as patellofemoral pain syndrome (PFPS) is a frequently encountered musculoskeletal disorder that worsens with activity. The multifactorial etiology of PFPS alters lower extremity mechanics, increasing patellofemoral joint stresses during weight-bearing tasks. Kinesio (KT) and McConnell (MC) tapings are often incorporated into the treatment, but their efficacy is still unclear. OBJECTIVES: To test the efficacy of KT, MC and sham taping (ST) in improving knee mechanics and reducing pain during activity...
December 11, 2018: Journal of Sport Rehabilitation
Lei Huang, Tong Peng, Yu Li, Yunyun He, Li Wang, Shiyan Zhang, Yiran Sun, Fang Chen, Lin Tang
Phytochemical investigation on the ethyl acetate extract of Idesia polycarpa Maxim. Leaves led to the isolation of four phenolic glycoside isomers (1-4). Compound 2 appeared to be new reported phenolic glycoside, while compound 1 was the first time isolated from the titled species. Their structures were established by IR, UV, HRESI-MS and 1D and 2D NMR spectroscopies analysis and comparison of spectral data with previously reported data. The compounds 3 and 4 showed stronger activity of scavenging the DPPH free radical than the other two compounds, while the compounds 1 and 2 showed a significant activity of scavenging the ABTS free radical...
December 9, 2018: Natural Product Research
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