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Mir-205-5p

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https://www.readbyqxmd.com/read/30050081/mir-205-5p-inhibition-by-locked-nucleic-acids-impairs-metastatic-potential-of-breast-cancer-cells
#1
Antonella De Cola, Alessia Lamolinara, Paola Lanuti, Cosmo Rossi, Manuela Iezzi, Marco Marchisio, Matilde Todaro, Vincenzo De Laurenzi
Mir-205 plays an important role in epithelial biogenesis and in mammary gland development but its role in cancer still remains controversial depending on the specific cellular context and target genes. We have previously reported that miR-205-5p is upregulated in breast cancer stem cells targeting ERBB pathway and leading to targeted therapy resistance. Here we show that miR-205-5p regulates tumorigenic properties of breast cancer cells, as well as epithelial to mesenchymal transition. Silencing this miRNA in breast cancer results in reduced tumor growth and metastatic spreading in mouse models...
July 26, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/30008858/mir-205-5p-inhibits-cell-migration-and-invasion-in-prostatic-carcinoma-by-targeting-zeb1
#2
Lianpeng Li, Shouqiang Li
Many studies have demonstrated that miRNAs have influence on tumorigenesis and progression of human cancers, including invasion and migration. Thus, the role of miR-205/ZEB1 axis for the migration and invasion of prostate cancer cells was explored in the present study. The miR-205-5p and zinc finger E-box binding homeobox 1 (ZEB1) mRNA expression levels were observed in prostate cancer tissues or cell lines via reverse transcription-quantitative PCR (RT-qPCR), and the protein level of ZEB1 was measured by western blotting...
August 2018: Oncology Letters
https://www.readbyqxmd.com/read/29812973/novel-prognostic-and-predictive-microrna-targets-for-triple-negative-breast-cancer
#3
Gulisa Turashvili, Elizabeth D Lightbody, Kathrin Tyryshkin, Sandip K SenGupta, Bruce E Elliott, Yolanda Madarnas, Abdi Ghaffari, Andrew Day, Christopher J B Nicol
Triple-negative breast cancers (TNBCs) account for ∼25% of all invasive carcinomas and represent a large subset of aggressive, high-grade tumors. Despite current research focused on understanding the genetic landscape of TNBCs, reliable prognostic and predictive biomarkers remain limited. Although dysregulated microRNAs (miRNAs) have emerged as key players in many cancer types, the role of miRNAs in TNBC disease progression is unclear. We performed miRNA profiling of 51 TNBCs by next-generation sequencing to reveal differentially expressed miRNAs...
May 29, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29783093/a-direct-plasma-mirna-assay-for-early-detection-and-histological-classification-of-lung-cancer
#4
Qixin Leng, Yue Wang, Feng Jiang
Cell-free microRNAs in plasma provide circulating biomarkers for lung cancer. Most techniques for analysis of miRNAs require a large plasma volume to purify a sufficient RNA yield followed by complicated downstream processing. Small differences in the multiple procedures often cause large analytical variations and poor diagnostic values of the plasma biomarkers. Here we investigate whether directly quantifying plasma miRNAs without RNA purification could diagnose lung cancer. FirePlex assay was directly applied to 20 μl plasma of 56 lung cancer patients and 28 cancer free controls for quantifying 11 lung tumor-associated miRNAs...
August 2018: Translational Oncology
https://www.readbyqxmd.com/read/29738771/mir-141-3p-is-upregulated-in-esophageal-squamous-cell-carcinoma-and-targets-pleckstrin-homology-domain-leucine-rich-repeat-protein-phosphatase-2-a-negative-regulator-of-the-pi3k-akt-pathway
#5
Osamu Ishibashi, Ichiro Akagi, Yota Ogawa, Takashi Inui
The phosphatidylinositol-3-kinase (PI3K)/AKT pathway is frequently activated in various human cancers and plays essential roles in their development and progression. Accumulating evidence suggests that dysregulated expression of microRNAs (miRNAs) is closely associated with cancer progression and metastasis. Here, we focused on miRNAs that could regulate genes related to the PI3K/AKT pathway in esophageal squamous cell carcinoma (ESCC). To identify upregulated miRNAs and their possible target genes in ESCC, we performed microarray-based integrative analyses of miRNA and mRNA expression levels in three human ESCC cell lines and a normal esophageal epithelial cell line...
June 22, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29727505/integrated-microrna-and-mrna-sequencing-analysis-of-age-related-changes-to-mouse-thymic-epithelial-cells
#6
Hong-Ling Jia, Xiao-Qin Zeng, Feng Huang, Ya-Meng Liu, Bi-Shuang Gong, Kai-Zhao Zhang, Jiang-Hui Zeng, Dong-Guang Guo, Zhuo-Ya Wang, Yu-Gu Li
MicroRNAs (miRNAs) play key roles in the regulation of gene expression during multiple physiological processes, including early development, differentiation, and ageing. However, their involvement in age-related thymic involution is not clear. In this study, we profiled the global transcriptome and miRNAome of thymic epithelial cells in 1- and 3-month-old male and female mice, and predicted the possible transcription factors and target genes of the four most significantly differentially expressed miRNAs (DEMs) (miR-183-5p, miR-199b-5p, miR-205-5p, and miR-200b-3p) by performing bioinformatics analyses...
July 2018: IUBMB Life
https://www.readbyqxmd.com/read/29667486/lncrna-adpgk-as1-promotes-pancreatic-cancer-progression-through-activating-zeb1-mediated-epithelial-mesenchymal-transition
#7
Suzhen Song, Weihua Yu, Sen Lin, Mingbao Zhang, Teng Wang, Shuang Guo, Hongbo Wang
OBJECTIVE: This study was conducted to investigate the effects of ADP dependent glucokinase antisense RNA 1 (ADPGK-AS1)/ miR-205-5p/ zinc finger E-box binding homeobox 1 (ZEB1) on PC cells. METHODS: Differentially expressed lncRNAs and miRNAs in pancreatic cancer (PC) were identified by microarray analysis. In silico ceRNA analysis was conducted to find out the interactions among lncRNAs, miRNAs and mRNAs. Quantitative real-time PCR (qRT-PCR) was utilized to examine the expression of miR-205-5p and lncRNA ADPGK-AS1 in PC and non-cancerous cells...
July 3, 2018: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/29574186/mir-205-5p-negatively-regulates-hepatic-acetyl-coa-carboxylase-%C3%AE-mrna-in-lipid-metabolism-of-oreochromis-niloticus
#8
Yi-Fan Tao, Jun Qiang, Jing-Wen Bao, Hong-Xia Li, Guo-Jun Yin, Pao Xu, De-Ju Chen
MicroRNAs (miRNAs) are non-coding RNAs that function as post-transcriptional gene regulators and that play vital roles controlling lipid metabolism. miR-205 is an important miRNA related to adipogenesis and lipid metabolism. However, little is known about the potential role of miR-205-5p in genetically improved farmed tilapia (GIFT, Oreochromis niloticus). In this study, we used miRanda software to search for potential miR-205-5p target genes and found a lipid-metabolism-related gene called acetyl-CoA carboxylase β (ACACβ)...
June 20, 2018: Gene
https://www.readbyqxmd.com/read/29556360/long-non-coding-mir205hg-depletes-hsa-mir-590-3p-leading-to-unrestrained-proliferation-in-head-and-neck-squamous-cell-carcinoma
#9
Silvia Di Agostino, Fabio Valenti, Andrea Sacconi, Giulia Fontemaggi, Matteo Pallocca, Claudio Pulito, Federica Ganci, Paola Muti, Sabrina Strano, Giovanni Blandino
Over 70% of head & neck squamous cell carcinoma (HNSCC) patients carry TP53 oncogenic mutations. Here we studied the role of specific tumor-derived mutant p53 proteins in the aberrant transcription of long non-coding (lnc) MIR205HG gene in head and neck cancer cells. Methods: To understand the role of lncMIR205HG, that we showed to be transcriptionally regulated by mutant p53 in HNSCC, we have employed siRNA and shRNA in CAL27 and FaDu HNSCC cell lines to suppress p53 gene expression in ChIP assays and RT-qPCR...
2018: Theranostics
https://www.readbyqxmd.com/read/29511451/lncrna-malat1-mir-205-5p-axis-regulates-mpp-induced-cell-apoptosis-in-mn9d-cells-by-directly-targeting-lrrk2
#10
Qin Chen, Xiaoyan Huang, Renjie Li
Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), as a long chain non-coding RNA (lncRNA), has been reported to be upregulated in Parkinson's disease (PD). However, the mechanisms underlying this process remain unknown. Hence, to investigate the role of MALAT1 in PD, N-methyl-4-phenylpyridinium (MPP+ ) was used to induce PD in vitro in the MN9D dopaminergic neuronal cell line and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was used to induce PD in vivo in C57BL/6 mice. Quantitative Real-Time PCR (qRT-PCR) and western blot assay showed that the expression levels of MALAT1 and leucine-rich repeat kinase (LRRK2) were increased, and that of miR-205-5p was decreased in the midbrains of mice in which PD was induced by MPTP...
2018: American Journal of Translational Research
https://www.readbyqxmd.com/read/29488611/mir%C3%A2-205%C3%A2-5p-ptk7-axis-is-involved-in-the-proliferation-migration-and-invasion-of-colorectal-cancer-cells
#11
Shuo Chen, Yan Wang, Yinan Su, Lin Zhang, Mingqing Zhang, Xueqing Li, Juan Wang, Xipeng Zhang
MicroRNAs (miRNAs) are small non‑coding RNAs, which are critical in a diverse range of biological processes, including development, differentiation, homeostasis, and in the formation of diseases by accelerating and/or inhibiting the translation of mRNAs. The present study aimed to examine the potential role of miRNA (miR)‑205‑5p in the developmental process of colorectal cancer (CRC) through protein‑tyrosine kinase 7 (PTK7). Initially, TargetScan was used to predict the miRNA target sites in the sequence of the PTK7 3'‑untranslated region...
May 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29464002/mir-205-5p-and-mir-342-3p-cooperate-in-the-repression-of-the-e2f1-transcription-factor-in-the-context-of-anticancer-chemotherapy-resistance
#12
Xin Lai, Shailendra K Gupta, Ulf Schmitz, Stephan Marquardt, Susanne Knoll, Alf Spitschak, Olaf Wolkenhauer, Brigitte M Pützer, Julio Vera
High rates of lethal outcome in tumour metastasis are associated with the acquisition of invasiveness and chemoresistance. Several clinical studies indicate that E2F1 overexpression across high-grade tumours culminates in unfavourable prognosis and chemoresistance in patients. Thus, fine-tuning the expression of E2F1 could be a promising approach for treating patients showing chemoresistance. Methods: We integrated bioinformatics, structural and kinetic modelling, and experiments to study cooperative regulation of E2F1 by microRNA (miRNA) pairs in the context of anticancer chemotherapy resistance...
2018: Theranostics
https://www.readbyqxmd.com/read/29393341/microrna-205-5p-suppresses-the-invasiveness-of-oral-squamous-cell-carcinoma-by-inhibiting-timp%C3%A2-2-expression
#13
Hiroki Nagai, Shogo Hasegawa, Fumihiko Uchida, Takehito Terabe, Naomi Ishibashi Kanno, Koroku Kato, Kenji Yamagata, Satoshi Sakai, Shuichi Kawashiri, Hiroshi Sato, Toru Yanagawa, Hiroki Bukawa
MicroRNAs (miRNAs or miRs) play important roles in carcinogenesis. The miRNA, miR-205-5p, has been reported to suppress the growth of various types of tumor; however, its functional contribution to oral squamous cell carcinoma (OSCC) is not yet clear. Thus, this study was conducted to determine the miRNA expression signatures in OSCC and to investigate the functional role of miR‑205‑5p in OSCC cells. We measured miR‑205‑5p expression by RT-qPCR, and examined the function of miR‑205‑5p by transfecting a miR‑205‑5p mimic or inhibitor into OSCC cells and measuring cell proliferation, migration and invasiveness...
March 2018: International Journal of Oncology
https://www.readbyqxmd.com/read/29391448/vasoactive-intestinal-peptide-gene-polymorphisms-associated-with-its-serum-levels-predict-treatment-requirements-in-early-rheumatoid-arthritis
#14
Iria V Seoane, Carmen Martínez, Rosario García-Vicuña, Ana M Ortiz, Yasmina Juarranz, Vanessa C Talayero, Isidoro González-Álvaro, Rosa P Gomariz, Amalia Lamana
We previously reported that early arthritis (EA) patients with low vasoactive intestinal peptide (VIP) serum levels demonstrate a worse clinical disease course. In this study, we analysed whether variants in the VIP gene correlated with its serum levels and clinical EA parameters. The VIP gene was sequenced in patients with extremely high/low VIP levels, measured by enzyme immunoassay. Sixteen single nucleotide polymorphisms (SNPs) were differentially distributed between both groups, which were subsequently genotyped in two patients' sets...
February 1, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29352232/the-silent-healer-mir-205-5p-up-regulation-inhibits-epithelial-to-mesenchymal-transition-in-colon-cancer-cells-by-indirectly-up-regulating-e-cadherin-expression
#15
Diana Gulei, Lorand Magdo, Ancuta Jurj, Lajos Raduly, Roxana Cojocneanu-Petric, Alin Moldovan, Cristian Moldovan, Adrian Florea, Sergiu Pasca, Laura-Ancuta Pop, Vlad Moisoiu, Liviuta Budisan, Cecilia Pop-Bica, Cristina Ciocan, Rares Buiga, Mihai-Stefan Muresan, Rares Stiufiuc, Calin Ionescu, Ioana Berindan-Neagoe
EMT represents the dominant program within advanced stages of colon cancer, where cells acquire migratory characteristics in order to invade secondary tissues and form metastasis. Where the majority of the therapeutic strategies are concentrated on the reduction of the tumor mass through different apoptotic mechanisms, the present study advocates an important role for miR-205-5p in impairment of colon cancer cells migration and restoration of the epithelial phenotype. Upon identification of a homogenous downregulated profile for miR-205-5p in colon adenocarcinoma patients, functional studies demonstrated that experimental upregulation of this sequence is able to significantly raise the levels of E-cadherin through direct inhibition of ZEB1...
January 19, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29344634/underlying-mechanism-of-the-photodynamic-activity-of-hematoporphyrin%C3%A2-induced-apoptosis-in-u87-glioma-cells
#16
Shi-Xiang Yuan, Jun-Liang Li, Xin-Ke Xu, Wei Chen, Cheng Chen, Kun-Qi Kuang, Fang-Yu Wang, Kai Wang, Fang-Cheng Li
Photodynamic therapy (PDT) is a relatively novel type of tumor therapy method with low toxicity and limited side‑effects. The aim of the present study was to investigate the underlying mechanism and potential microRNAs (miRNAs) involved in the treatment of glioma by PDT with hematoporphyrin, a clinical photosensitizer. The photodynamic activity of hematoporphyrin on the cell viability and apoptosis of gliomas was investigated by MTT, and flow cytometry and fluorescence microscopy, respectively. Alterations in singlet oxygen and mitochondrial membrane potential were detected...
January 18, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29295562/oncomirs-expression-profiling-in-uterine-leiomyosarcoma-cells
#17
Bruna Cristine de Almeida, Natalia Garcia, Giovana Maffazioli, Laura Gonzalez Dos Anjos, Edmund Chada Baracat, Katia Candido Carvalho
MicroRNAs (miRNAs) are small non-coding RNAs that act as regulators of gene expression at the post-transcriptional level. They play a key role in several biological processes. Their abnormal expression may lead to malignant cell transformation. This study aimed to evaluate the expression profile of 84 miRNAs involved in tumorigenesis in immortalized cells of myometrium (MM), uterine leiomyoma (ULM), and uterine leiomyosarcoma (ULMS). Specific cell lines were cultured and qRT-PCR was performed. Thirteen miRNAs presented different expression profiles in ULM and the same thirteen in ULMS compared to MM...
December 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29224008/mirnas-mediate-gdnf-induced-proliferation-and-migration-of-glioma-cells
#18
Bao-Le Zhang, Fu-Lu Dong, Ting-Wen Guo, Xiao-He Gu, Lin-Yan Huang, Dian-Shuai Gao
BACKGROUND/AIMS: Glial cell line-derived neurotrophic factor (GDNF) is an important factor promoting invasive glioma growth. This study was performed to reveal a unique mechanism of glioma cell proliferation and migration. METHODS: Human U251 glioma cells were used to screen the optimal GDNF concentration and treatment time to stimulate proliferation and migration. MicroRNA (MiRNA) expression profiles were detected by microarray and confirmed by real-time polymerase chain reaction (PCR)...
2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29196733/regulation-of-hmgb3-by-antitumor-mir-205-5p-inhibits-cancer-cell-aggressiveness-and-is-involved-in-prostate-cancer-pathogenesis
#19
Yasutaka Yamada, Rika Nishikawa, Mayuko Kato, Atsushi Okato, Takayuki Arai, Satoko Kojima, Kazuto Yamazaki, Yukio Naya, Tomohiko Ichikawa, Naohiko Seki
Our recent determination of a microRNA (miRNA) expression signature in prostate cancer (PCa) revealed that miR-205-5p was significantly reduced in PCa tissues and that it acted as an antitumor miRNA. The aim of this study was to identify oncogenic genes and pathways in PCa cells that were regulated by antitumor miR-205-5p. Genome-wide gene expression analyses and in silico miRNA database searches showed that 37 genes were putative targets of miR-205-5p regulation. Among those genes, elevated expression levels of seven in particular (HMGB3, SPARC, MKI67, CENPF, CDK1, RHOU, and POLR2D) were associated with a shorter disease-free survival in a large number of patients in the The Cancer Genome Atlas (TCGA) database...
February 2018: Journal of Human Genetics
https://www.readbyqxmd.com/read/29187807/characteristic-mirna-expression-signature-and-random-forest-survival-analysis-identify-potential-cancer-driving-mirnas-in-a-broad-range-of-head-and-neck-squamous-cell-carcinoma-subtypes
#20
Yury O Nunez Lopez, Berta Victoria, Pawel Golusinski, Wojciech Golusinski, Michal M Masternak
Aim: To characterize the miRNA expression profile in head and neck squamous cell carcinoma (HNSSC) accounting for a broad range of cancer subtypes and consequently identify an optimal miRNA signature with prognostic value. Background: HNSCC is consistently among the most common cancers worldwide. Its mortality rate is about 50% because of the characteristic aggressive behavior of these cancers and the prevalent late diagnosis. The heterogeneity of the disease has hampered the development of robust prognostic tools with broad clinical utility...
January 2018: Reports of Practical Oncology and Radiotherapy
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