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Azizah Al-Anazi, Ranjit Parhar, Soad Saleh, Reem Al-Hijailan, Angela Inglis, Mansour Al-Jufan, Mohammed Bazzi, Sarwar Hashmi, Walter Conca, Kate Collison, Futwan Al-Mohanna
The data set presented here is associated with the article "Intracellular calcium and NF-k B regulate hypoxia-induced leptin, VEGF, IL-6 and adiponectin secretion in human adipocytes" (Al-Anazi et al., 2018). Data illustrate hypoxia-induced VEGF and leptin expression in human adipocytes treated with the calcium chelator BAPTA-AM (1 µM). It also shows NF-κB p65 induced expression by hypoxia. Preadipocytes were differentiated for 14 days and then subjected to 0.5-1.5% oxygen in the presence and absence of BAPTA-AM or the NF-κB inhibitor SN50 for 48 h prior to RNA isolation and PCR analysis...
December 2018: Data in Brief
Tiziana Triulzi, Luca Forte, Viola Regondi, Martina Di Modica, Cristina Ghirelli, Maria Luisa Carcangiu, Lucia Sfondrini, Andrea Balsari, Elda Tagliabue
Through whole-transcriptome profiling of HER2+ breast carcinomas (BCs), we previously showed that those sensitive to trastuzumab are addicted to this oncoprotein and are enriched in immune pathways, raising the hypothesis that HER2 itself regulates immune cell recruitment. In the present study we investigated the relationship between HER2 activity and the pro-trastuzumab tumor immune milieu. Gene expression profiling and immunohistochemistry analysis of 53 HER2+ BCs showed that trastuzumab-sensitive tumors expressed significantly higher levels of chemokines involved in immune cell recruitment, with higher infiltration of T cells and monocytes, and higher levels of PD-1 ligands than tumors that do not benefit from trastuzumab...
2019: Oncoimmunology
Giacomo Pepe, Eduardo Sommella, Donato Cianciarulo, Carmine Ostacolo, Michele Manfra, Veronica Di Sarno, Simona Musella, Mariateresa Russo, Antonella Messore, Barbara Parrino, Alessia Bertamino, Giuseppina Autore, Stefania Marzocco, Pietro Campiglia
Citrus fruits are often employed as ingredients for functional drinks. Among Citrus , the variety, "Lempso", a typical hybrid of the Calabria region (Southern Italy), has been reported to possess superior antioxidant activity when compared to other common Citrus varieties. For these reasons, the aim of this study is to investigate in vitro the nutraceutical value of the Tarocco clone, "Lempso", highlighting its anti-inflammatory and antioxidant potential. A post-column 2,2'-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging assay for the screening of antioxidant compounds in these complex matrices was developed...
December 11, 2018: Nutrients
Meghan J Bloom, Sachit D Saksena, George P Swain, Marcelo S Behar, Thomas E Yankeelov, Anna G Sorace
Small molecule approaches targeting the nuclear factor kappa B (NF-kB) pathway, a regulator of inflammation, have thus far proven unsuccessful in the clinic in part due to the complex pleiotropic nature of this network. Downstream effects depend on multiple factors including stimulus-specific temporal patterns of NF-kB activity. Despite considerable advances, genome-level impact of changes in temporal NF-kB activity caused by inhibitors and their stimulus dependency remains unexplored. This study evaluates the effects of pathway inhibitors on early NF-κB activity and downstream gene transcription...
December 10, 2018: Cellular Signalling
Qianqian Xu, Junsong Guo, Weidong Chen
Gambogenic acid (GNA), an active ingredient isolated from Gamboge, which possesses diverse antitumor effects in vivo and vitro. Here we were mainly designed to understand the role of GNA in drug resistance in HepG2/Adr cells. The alteration of cytotoxic drugs IC50 was examined using the MTT method. Cell apoptosis and uptake of P-glycoprotein (P-gp) substrates were measured under a flow cytometry and fluorescence microscope, respectively. Moreover, the ATPase activity, the expression of P-gp and P-gp-related proteins were also investigated...
December 8, 2018: Biochemical and Biophysical Research Communications
Weifeng Huang, Yongmei Cao, Yujing Liu, Feng Ping, Jiawei Shang, Zhongwei Zhang, Yingchuan Li
The balance between Ang II/AT1R and Ang-(1-7)/Mas plays a pivotal role in the development of lipopolysaccharides (LPS)-induced acute respiratory distress syndrome. However, the mechanisms underlying the balancing process still remain unclear. Here we investigated the roles of nuclear factor (NF)-κB and p53 in regulating AT1R and Mas expression. The results demonstrated that Ang II pretreatment resulted in downregulation of Mas and upregulation of AT1R, phosphorylated p65, and apoptosis in LPS-treated Human pulmonary microvascular endothelial cells (HPMVECs), but had no effect on p53 expression...
December 7, 2018: Journal of Cellular Physiology
Baochi Ou, Yuan Liu, Tao Zhang, Yahuang Sun, Jiayi Chen, Zhihai Peng
BACKGROUND: There are limited markers that could facilitate individualized tacrolimus treatment in the early posttransplantation period. Genetic factors have been found to play critical roles in determining tacrolimus pharmacokinetics. OBJECTIVE: We aimed to examine the association of donor and recipient Toll-like receptor (TLR) polymorphisms with tacrolimus elimination and the potential mechanism for TLR gene polymorphism-mediated tacrolimus metabolism. METHODS: Two independent cohorts including 297 patients receiving liver transplantation (LT) were enrolled in this study (Cohort A was comprised of 200 patients; cohort B included 97 patients and served as a validation set...
December 8, 2018: Pharmacotherapy
Bilal Ahmad, Muneeb U Rehman, Insha Amin, Manzoor Ur Rahman Mir, Sheikh Bilal Ahmad, Adil Farooq, Showkeen Muzamil, Ishraq Hussain, Mubashir Masoodi, Bilques Fatima
Diabetes is considered as the most common metabolic disease affecting millions of people all around the world. Use of natural herbal medicines can be effective in treating diabetes. Zingerone (4-(4-hydroxy-3-methylphenyl) butan-2-one) a polyphenolic alkanone extracted from ginger has a broad spectrum of pharmacological properties and thus can be used as a promising candidate against various ailments. In the current study we aimed at demonstrating the protective effect of zingerone against diabetes mellitus and elucidating its possible mechanism...
December 2018: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
Titus Chukwuemeka Obasi, Cornelia Braicu, Bogdan Cezar Iacob, Ede Bodoki, Ancuta Jurj, Lajos Raduly, Ilioara Oniga, Ioana Berindan-Neagoe, Radu Oprean
Introduction: Scientific research is beginning to prove the connection between claims by African traditional medicine and the natural chemical specifics contained in medicinal plant Securidaca longipedunculata . Our previous studies showed that two natural saponin fractions (4A3 and 4A4) identified in the plant as triterpenoid glycosides are capable of activating apoptosis on cervical tumor cell lines. Considering this and some critical roles of human papillomavirus (HPV) E6 oncogene on cervical cells, by promoting carcinogenesis and cell survival, it became necessary to investigate the possible pathways for apoptosis transmission...
2018: Cancer Management and Research
Alla G Guzii, Tatyana N Makarieva, Vladimir A Denisenko, Pavel S Dmitrenok, Roman S Popov, Alexandra S Kuzmich, Sergey N Fedorov, Vladimir B Krasokhin, Natalya Yu Kim, Valentin A Stonik
Melonoside B (1) and melonosins B (2) and A (3), new lipids based on polyoxygenated fatty acid amides, and known melonoside A (4) were isolated from two different collections of the marine sponge Melonanchora kobjakovae. The structures of these compounds, including their absolute configurations, were established using detailed analysis of 1D and 2D NMR, ECD, and mass spectra as well as chemical transformations. Melonosins 2 and 3 inhibit AP-1- and NF-kB-dependent transcriptional activities in JB6 Cl41 cells at noncytotoxic concentrations, demonstrating potential cancer preventive activity...
December 7, 2018: Journal of Natural Products
Sun-Hyung Ha, Kyung-Min Kwon, Jun-Young Park, Fukushi Abekura, Young-Choon Lee, Tae-Wook Chung, Ki-Tae Ha, Hyeun Wook Chang, Seung-Hak Cho, Jong-Suk Kim, Cheorl-Ho Kim
A water-soluble saponin, Esculentoside H (EsH), 3-O-(O-β-d-glucopyranosyl-(1→4)-β-d-xylopyranosyl)-28-β-d-glucopyranosylphytolaccagenin has been isolated and purified from the root extract of perennial plant Phytolacca esculenta. EsH is known to be an anticancer compound, having a capacity for TNF-α release. However, the effects of EsH on migration and growth in tumor cells have not yet been reported. In the current study, the suppressive effects of EsH on phorbol 12-myristate 13-acetate (PMA)-induced cell migration were examined in murine colon cancer CT26 cells and human colon cancer HCT116 cells...
December 7, 2018: Journal of Cellular Biochemistry
Yi Wang, Ting Xie, Dan Zhang, Po Sing Leung
G-protein coupled receptor 120 (GPR120) has been shown to act as an omega-3 unsaturated fatty acid sensor and is involved in insulin secretion. However, the underlying mechanism in pancreatic beta cells remains unclear. To explore the potential link between GPR120 and beta-cell function, its agonist DHA and GSK137647AS were used in palmitic acid (PA)-induced pancreatic beats-cell dysfunction, coupled with GPR120 KD in MIN6 cells and GPR120 KO mice to identify the underlying signalling pathways. In vitro and ex vivo treatments of MIN6 cells and islets isolated from WT mice with DH and GSK restored pancreatic PDX1 expression levels and beta-cell function via PA-induced elevation of proinflammatory chemokine and activation of NF-kB, JNK and p38MAPK signalling pathways...
December 6, 2018: Clinical Science (1979-)
Qiang Ma, Yin-Sheng Wu, Jiang-Yong Shen, Zhi-Bin Yang, Hong-Xia Shen, Miao Yao, Chun-Hua Yu
The effects of walnut oil on wound healing and skin injury repair was observed in Sprague-Dawley (SD) rats, and mechanism of action was investigated. Normal SD rats were divided into an experimental group and a control group. Each group was observed at4 time points (day [D]3, D7, D14, and D21). In both groups, a skin wound was created on the back of the rats, with the spine as the central axis. In the experimental group, the wound was covered with walnut oil, and then bandaged and fixed with sterile gauze. In the control group, the wound was bandaged with vaseline gauze...
December 1, 2018: Die Pharmazie
Muhammad Imran Khan, Jin Hyuk Shin, Tai Sun Shin, Min Yong Kim, Nam Jun Cho, Jong Deog Kim
Cornus kousa the Korean dogwood has been traditionally used in East Asia as therapeutic traditional medicine however biological activities of Cornus kousa have not been investigated previously. The aim of the present study was to evaluate anti-obesity activities coupled with anti-angiogenic activities of anthocyanins rich fraction of ethanolic leaf extract of Cornus kousa (ELECk) in HUVECs and 3T3- L1 cells. Dried plants leaves were extracted with 70% ethanol and anthocyanin fraction (AnT Fr) was obtained by eluting the ethanolic extract through non-polar macroporous resin and further purification by HPLC...
2018: PloS One
He Liang, Xinjian Yang, Chao Liu, Zhongyi Sun, Xiaobin Wang
OBJECTIVE: Τo investigate the effect of NF-kB signaling pathway on the expression of MIF, TNF-α, and IL-6 in the regulation of disc degeneration. METHODS: The disc tissue was taken from 56 patients with cervical spondylosis. According to the preoperative MRI and intraoperative disc herniation, the patients were divided into two groups: degeneration group and herniation group. The control group was 34 patients with cervical trauma with no history of cervical spondylosis...
December 1, 2018: Journal of Musculoskeletal & Neuronal Interactions
Benjamin Y Klein, Hadassah Tamir, Muhammad Anwar, Robert J Ludwig, Jasmine H Kaidbey, Sara B Glickstein, Martha G Welch
The goal of this protocol is to isolate oxytocin-receptor rich brain nuclei in the neonatal brain before and after first colostrum feeding. The expression of proteins known to respond to metabolic stress was measured in brain-nuclei isolates using Western blotting. This was done to assess whether metabolic stress-induced nutrient insufficiency in the body triggered neuronal stress. We have previously demonstrated that nutrient insufficiency in neonates elicits metabolic stress in the gut. Furthermore, colostrum oxytocin modulates cellular stress response, inflammation, and autophagy markers in newborn rat gut villi prior to and after first feed...
November 14, 2018: Journal of Visualized Experiments: JoVE
Baban S Thawkar, Ginpreet Kaur
Microglial activation is a distinguished attribute in many neurodegenerative diseases of aging. Compelling evidence suggests that neuroinflammation stimulated by microglia, the resident macrophage-like immune cells in the brain, play a contributing role in the pathogenesis of Alzheimer's disease (AD). Postmortem brain tissue of individuals with AD has credibly demonstrated that neuroinflammation is likely to be a key driver of the disease. Recently, It has been found that manipulating β-amyloid directly is an impracticable approach for therapeutic intervention due to the failure of β-amyloid-lowering drugs in clinical trials...
November 20, 2018: Journal of Neuroimmunology
Yin Chaoyang, Bai Qingfeng, Feng Jinxing
Asthma is a complex, chronic inflammatory disorder of the bronchial tree, and can affect patients of all ages including children. High mobility group box 1 (HMGB1) has been proved as a therapeutic target in children with asthma, and was predicted to be the target gene of microRNA-216a-5p (miR-216a-5p). The present study aimed to investigate the function of miR-216a-5p in asthma by creating a human bronchial epithelial cell (16HBE) injury model using H₂O₂. A significantly elevation of HMGB1 protein expression and a reduction of miR-216a-5p expression were observed in children with asthma as well as in H₂O₂ stimulated 16HBE cells...
November 27, 2018: Biochemical and Biophysical Research Communications
Andrii Bazylevich, Helena Tuchinsky, Eti Zigman-Hoffman, Ran Weissman, Ofer Shpilberg, Oshrat Hershkovitz-Rokah, Leonid Patsenker, Gary Gellerman
BACKGROUND: Scientists have extensively investigated curcumin, yielding many publications on treatments of cancer. Numerous derivatives of curcumin were synthesized, evaluated for their anti-oxidant and free-radical scavenging, SAR, ADME properties and tested in anticancer applications. OBJECTIVE: We decided to exploit curcumin as a bioactive core platform for carrying anticancer drugs, which likely possesses a carboxyl moiety for potential linkage to the carrier for drug delivery...
December 2, 2018: Medicinal Chemistry
Mohammed Youssef, Ahmed Ibrahim, Koichi Akashi, Md Shamim Hossain
The special lipids Plasmalogens (Pls) were reported to be reduced in the neurodegenerative brains such as Alzheimer's disease where a marked increase of glial activation is often observed. We previously found that a reduction of brain Pls can enhance the glial activation in murine brains. However, the detail role of Pls in the prevention of glial activation was mostly elusive. Here we report that the Pls, extracted from scallop (sPls), significantly inhibited the inducible form of nitric oxide synthase (NOS2) and the production of NO in LPS (lipopolysaccharide)-activated microglial cells...
November 26, 2018: Neuroscience
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