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Lijun Yang, Kaiyan Sun, Jie Chu, Yunhui Qu, Xue Zhao, Huiqing Yin, Liang Ming, Junhu Wan, Fucheng He
AIMS:: Recent research showed that Long non-protein coding RNA ferritin heavy chain 1 pseudogene 3 (FTH1P3) plays a crucial role in the course of tumor formation. The present study was aimed to explore its role in esophageal squamous cell carcinoma (ESCC). MAIN METHODS: Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was used to examine the expression levels of FTH1P3, mRNA SP1 and NF-kB in ESCC samples and cell lines. The impact of FTH1P3 knockdown was evaluated by WST-1 assays, colony formation assays, scratch wound assays, migration and invasion assays...
July 27, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Khaled F Al-Massri, Lamiaa A Ahmed, Hanan S El-Abhar
Anticonvulsant drugs such as pregabalin (PGB) and lacosamide (LCM), exhibit potent analgesic effects in diabetic neuropathy; however, their possible role/mechanisms in paclitaxel (PTX)-induced peripheral neuropathy have not been elucidated, which is the aim of the present study. Neuropathic pain was induced in rats by injecting PTX (2 mg/kg, i. p) on days 0, 2, 4 and 6. Forty eight hours after the last dose of PTX, rats were treated orally with 30 mg/kg/day of either PGB or LCM for 21 days. Both therapies improved thermal hyperalgesia and cold allodynia induced by PTX...
August 14, 2018: Neurochemistry International
Dariush Sheikholeslami-Vatani, Slahadin Ahmadi, Hassan Faraji
The potential benefits of Omega-3 fatty acids and branched-chain amino acids (BCAAs) supplements on exercise-induced apoptosis are not clear. In a crossover randomized study, 11 men (age 62.8 ± 2.2 y) performed an acute bout of resistance exercise and underwent one-week supplementation with either 20 grams BCAA or 2700 mg omega-3/day. Subjects performed the same exercise after supplementation protocols. Following a three-week washout period, subjects switched groups. Circulating levels of soluble Fas ligand (sFasL), cytochrome c, Bax, BCL-2, and NF-kB were measured before and immediately after exercise sessions...
August 17, 2018: Journal of Aging and Physical Activity
J H Azambuja, N E Gelsleichter, L R Beckenkamp, I C Iser, M C Fernandes, F Figueiró, A M O Battastini, J N Scholl, F H de Oliveira, R M Spanevello, Jean Sévigny, M R Wink, M A Stefani, H F Teixeira, Elizandra Braganhol
Glioblastoma is the worst and most common primary brain tumor. Here, we demonstrated the role of CD73, an enzyme responsible for adenosine (ADO) production, in glioblastoma progression. ADO increased glioma cell viability via A1 receptor sensitization. CD73 downregulation decreased glioma cell migration and invasion by reducing metalloproteinase-2 and vimentin expression and reduced cell proliferation by 40%, which was related to necrosis and sub-G1 phase blockage of cell cycle. Those effects also involved the stimulation of Akt/NF-kB pathways...
August 16, 2018: Molecular Neurobiology
Nahuel A Ramella, Isabel Andújar, José L Ríos, Silvana A Rosú, M Alejandra Tricerri, Guillermo R Schinella
The cascade of molecular events leading to Human apolipoprotein A-I (apoA-I) amyloidosis is not completely understood, not even the pathways that determine clinical manifestations associated to systemic protein deposition in organs such as liver, kidney and heart. About twenty natural variants of apoA-I were described as inducing amyloidosis, but the mechanisms driving their aggregation and deposition are still unclear. We previously identified that the mutant Gly26Arg but not Lys107-0 induced the release of cytokines and reactive oxygen species from cultured RAW 264...
August 9, 2018: Pathophysiology: the Official Journal of the International Society for Pathophysiology
Longhe Xu, Yaobo Liu, Yuhui Sun, Hao Li, Weidong Mi, Yuge Jiang
OBJECTIVE: Chronic neuropathic pain (CNP) is attributed to a lesion or disease of the somatosensory system, may be derived from the peripheral and central system. Recent study revealed that spinal cord stimulation attenuated CNP by inhibiting TLR4/NF-κB signaling pathway. The present study focuses on the potential analgesic effects of TLR4/NF-κB signaling pathway on CNP in a rat model of chronic constriction injury (CCI). METHODS: We successfully established the rat model of CCI by Bennett method, and then inhibited the TLR4/NF-κB signaling pathway in rat models...
August 13, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
S Haghjooy-Javanmard, A Ghasemi, I Laher, B Zarrin, N Dana, G Vaseghi
Morphine affects the risk of metastasis in cancer. The TLR4 gene promotes migration in adenocarcinoma cells. We investigated the effect of morphine on TLR4, MyD88 and NF-κ B-expression and migration. Migration of estrogen receptor-positive MCF7 breast cancer cells was studied after 24 and 48 hours incubation with morphine, with boyden chamber method. Morphine effect on TLR4, MyD88 and NF-κB mRNA expression was determined by quantitative Real-Time polymerase chain reaction. Migration was reduced at the doses of 0...
2018: Bratislavské Lekárske Listy
Jennifer L Welch, Hussein Kaddour, Patrick M Schlievert, Jack T Stapleton, Chioma M Okeoma
Exosomes play various roles in host responses to cancer and infective agents, and semen exosomes (SE) inhibit HIV-1 infection and transmission, although the mechanism(s) by which this occurs is unclear. Here, we show that SE block HIV-1 proviral transcription at multiple transcriptional check points including transcription factor recruitment to the LTR, and transcription initiation and elongation. Biochemical and functional studies show that SE inhibit HIV-1 long terminal repeat (HIV-LTR)-driven viral gene expression and virus replication...
August 15, 2018: Journal of Virology
Lijuan Chai, Kun Zhou, Shaoxia Wang, Han Zhang, Na Fan, Jie Li, Xiaofeng Tan, Limin Hu, Xiang Fan
BACKGROUND/AIMS: Psoralen and bakuchiol are the main active compounds found in the traditional Chinese medicine Psoralea corylifolia L., and have been used to treat osteoporosis. This study aims to investigate the anti-osteoporosis effects of these two compounds using osteoclasts precursor differentiation and bone absorption assays in vitro. METHODS: Primary mouse osteoclasts precursor cells were induced by M-CSF (macrophage colony stimulating factor) plus RANKL (receptor activator of nuclear factor kappa-B ligand) in vitro...
August 15, 2018: Cellular Physiology and Biochemistry
Martien van Wenum, Philipp Treskes, Aziza A A Adam, Vincent A van der Mark, Aldo Jongejan, Perry D Moerland, Thomas M van Gulik, Ronald P J Oude Elferink, Robert A F M Chamuleau, Ruurdtje Hoekstra
BACKGROUND/AIMS: For applicability of cell-based therapies aimed at the treatment of liver failure, such as bioartificial livers (BALs) and hepatocyte transplantation, it is essential that the applied hepatocytes tolerate exposure to the patient plasma. However, plasma from both healthy donors and acute liver failure (ALF) patients is detrimental to hepatocytes and hepatic cell lines, such as HepaRG. We aimed to elucidate the underlying mechanisms of plasma-induced toxicity against HepaRG cells in order to ultimately develop methods to reduce this toxicity and render HepaRG-BAL treatment more effective...
August 15, 2018: Cellular Physiology and Biochemistry
Ercan Yilmaz, Mehmet Gul, Rauf Melekoglu, Isil Koleli
PURPOSE: To investigate the place of the transcription factor nuclear kappa B (NF-kB), which is a marker of chronic inflammation, in the etiology of the ovarian carcinoma. METHODS: NFkB analysis with the immunohistochemical method has been performed. To evaluate immunohistochemical NF-kB expression in the ovarian tissue, the H-score method. H-score = ∑ Pi (i+1), where ''Pi'' is the percentage of stained cells in each intensity category (0-100%) and ''i'' is the intensity indicating weak (i=1), moderate (i=2) or strong staining (i=3)...
July 2018: Acta Cirúrgica Brasileira
Chenglong Liu, Fei Zhang, Haihua Liu, Fang Wei
PURPOSE: To evaluate the role of CX3CL1 and NF-κB in the lumbar disc herniation induced neuropathic pain. METHODS: After LDH induced by implantation of autologous nucleus pulposus (NP) on the left L5 nerve root was established, mechanical thresholds and thermal hyperalgesia were tested at relevant time points during an observation period of 28 days. Expression of CX3CL1 and NF-κBin the dorsal root ganglion (DRG) were performed by using Western blotting and RT-PCR...
July 2018: Acta Cirúrgica Brasileira
Yunzhong Qian, Yi Chen, Linyang Wang, Jinfa Tou
PURPOSE: To investigate the effects of baicalin on inflammatory reaction, oxidative stress and protein kinase D1 (PKD1) and nuclear factor-kappa B (NF-κB) protein expressions in severe acute pancreatitis (SAP) rats. METHODS: Sixty rats were divided into sham operation, model, and low-, medium- and high-dose baicalin group. SAP model was established in later 4 groups. The later 3 groups were injected with 0.1, 0.2 and 0.4 ml/100 g 5% baicalin injection, respectively...
July 2018: Acta Cirúrgica Brasileira
Agnese C Pippione, Stefano Sainas, Antonella Federico, Elisa Lupino, Marco Piccinini, Michael Kubbutat, Jean-Marie Contreras, Christophe Morice, Alessandro Barge, Alex Ducime, Donatella Boschi, Salam Al-Karadaghi, Marco L Lolli
NF-κB-inducing kinase (NIK), an oncogenic drug target that is associated with various cancers, is a central signalling component of the non-canonical pathway. A blind screening process, which established that amino pyrazole related scaffolds have an effect on IKKbeta, led to a hit-to-lead optimization process that identified the aminopyrazole 3a as a low μM selective NIK inhibitor. Compound 3a effectively inhibited the NIK-dependent activation of the NF-κB pathway in tumour cells, confirming its selective inhibitory profile...
June 1, 2018: MedChemComm
Chetan Kumar, Reyaz Ur Rasool, Zainab Iqra, Yedukondalu Nalli, Prabhu Dutt, Naresh K Satti, Neha Sharma, Sumit G Gandhi, Anindya Goswami, Asif Ali
Herein, we report the isolation and synthetic modification of dehydrozingerone (DHZ, 1 ), a secondary metabolite present in the rhizome of Zingiber officinale . We synthesized O -propargylated dehydrozingerone, which was subsequently coupled by alkyne-azide cycloaddition ( 3-20 ) using click chemistry. The compounds ( 1-20 ) were evaluated for their in vitro cytotoxic activity in a panel of three cancer cell lines. Among all the DHZ derivatives, 3 , 6 , 7 , 8 , 9 and 15 displayed potent cytotoxic potential with an IC50 value ranging from 1...
November 1, 2017: MedChemComm
Enrico Gugliandolo, Ramona D'Amico, Marika Cordaro, Roberta Fusco, Rosalba Siracusa, Rosalia Crupi, Daniela Impellizzeri, Salvatore Cuzzocrea, Rosanna Di Paola
Traumatic brain injuries (TBI) are an important public health challenge. In addition, subsequent events at TBI can compromise the quality of life of these patients. In fact, TBI is associated with several complications for both long and short term, some evidence shows how TBI is associated with a decline in cognitive functions such as the risk of developing dementia, cerebral atrophy, and Parkinson disease. After the direct damage from TBI, a key role in TBI injury is played by the inflammatory response and oxidative stress, that contributes to tissue damage and to neurodegenerative processes, typical of secondary injury, after TBI...
2018: Frontiers in Neurology
Seunghwan Choi, Minsik Park, Joohwan Kim, Wonjin Park, Suji Kim, Dong-Keon Lee, Jong Yun Hwang, Jongseon Choe, Moo-Ho Won, Sungwoo Ryoo, Kwon-Soo Ha, Young-Guen Kwon, Young-Myeong Kim
cGMP-dependent protein kinase 1 (PKG1) plays an important role in nitric oxide (NO)/cGMP-mediated maintenance of vascular smooth muscle cell (VSMC) phenotype and vasorelaxation. Inflammatory cytokines, including tumor necrosis factor-α (TNF-α), have long been understood to mediate several inflammatory vascular diseases. However, the underlying mechanism of TNF-α-dependent inflammatory vascular disease is unclear. Here, we found that TNF-α treatment decreased PKG1 expression in cultured VSMCs, which correlated with NF-κB-dependent biogenesis of miR-155-5p that targeted the 3'-untranslated region of PKG1 mRNA...
August 13, 2018: Journal of Biological Chemistry
Breetha R, Ramaprasad Tr
This study addresses the effects of n-3 and n-6 fatty acids in maternal dyslipidemia induced inflammation over three generation in rats. The detailed protocol for animal feeding and mating is described in the methodology. Placenta and fetal liver were isolated on the eighteenth day of gestation and delivered pups after lactation were kept on their maternal diets. Compared to control and experimental groups, high-fat fed rats (HFL) had a higher level of cytokines and eicosanoids in serum (p < 0.05). Liver and uterine expression of cPLA-2, Cox-2, 5-Lox, EP-1, BLT-1, and ICAM-1 were higher (p < 0...
August 2018: Prostaglandins, Leukotrienes, and Essential Fatty Acids
M K Seo, E J Park, S Y Ko, E W Choi, S Kim
Kefir is a fermented product from yeast and lactic acid bacteria, and has been associated with various health benefits including relieving inflammatory bowel disease. Recently, it has been shown that gram-positive bacteria produce extracellular vesicles (EV). The EV could be appearing as potentially important mediators of cell to cell interaction. In this study, we explored the role of kefir grain Lactobacillus-derived EV in modulating inflammation responses via alleviating the production of inflammatory cytokines in tumor necrosis factor-α (TNF-α)-induced inflammation in Caco-2 cells and the 2,4,6-trinitrobenzene sulfonic acid-induced inflammatory bowel disease mouse model...
August 9, 2018: Journal of Dairy Science
Yan Zhang, Xiaoli Su, Fangfang Zou, Tengjuan Xu, Pinhua Pan, Chengping Hu
BACKGROUND: Obstructive sleep apnea (OSA)-associated chronic kidney disease is mainly caused by chronic intermittent hypoxia (CIH) triggered renal damage. This study aims to investigate the role of toll-like receptor-4 (TLR4) in underlying mechanism involved chronic intermittent hypoxia (CIH)-induced renal damage. METHODS: C57BL/6J mice with normal TLR4 (TLR4 WT) or deficient TLR4 (TLR4 KO) were divided into four groups and exposed to normal air (NA) and CIH: TLR4 WT + NA, TLR4 KO + NA, TLR4 WT + CIH, and TLR4 KO + CIH...
August 11, 2018: Sleep & Breathing, Schlaf & Atmung
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