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Keywords
Proteomic , serum marker, canc...
Proteomic , serum marker, cancer detection, cancer metastasis
#1
JOURNAL ARTICLE
Arba Karcini, Iulia M Lazar
The plasma membrane proteome resides at the interface between the extra- and intra-cellular environment and through its various roles in signal transduction, immune recognition, nutrient transport, and cell-cell/cell-matrix interactions plays an absolutely critical role in determining the fate of a cell. Our work was aimed at exploring the cell-membrane proteome of a HER2+ breast-cancer cell line (SKBR3) to identify triggers responsible for uncontrolled cell proliferation and intrinsic resources that enable detection and therapeutic interventions...
June 27, 2022: Scientific Reports
#2
JOURNAL ARTICLE
Camila Espejo, Richard Wilson, Eduard Willms, Manuel Ruiz-Aravena, Ruth J Pye, Menna E Jones, Andrew F Hill, Gregory M Woods, A Bruce Lyons
The iconic Tasmanian devil (Sarcophilus harrisii) is endangered due to the transmissible cancer Devil Facial Tumour Disease (DFTD), of which there are two genetically independent subtypes (DFT1 and DFT2). While DFT1 and DFT2 can be differentially diagnosed using tumour biopsies, there is an urgent need to develop less-invasive biomarkers that can detect DFTD and distinguish between subtypes. Extracellular vesicles (EVs), the nano-sized membrane-enclosed vesicles present in most biofluids, represent a valuable resource for biomarker discovery...
December 2021: Cellular and Molecular Life Sciences: CMLS
#3
JOURNAL ARTICLE
Christine Goudsmit, Felipe da Veiga Leprevost, Venkatesha Basrur, Lila Peters, Alexey Nesvizhskii, Heather Walline
To identify potential extracellular vesicle (EV) biomarkers in head and neck squamous cell carcinoma (HNSCC), we evaluated EV protein cargo and whole cell lysates (WCL) from HPV-positive and -negative HNSCC cell lines, as well as normal oral keratinocytes and HPV16-transformed cells. EVs were isolated from serum-depleted, conditioned cell culture media by polyethylene glycol (PEG) precipitation/ultracentrifugation. EV and WCL preparations were analyzed by LC-MS/MS. Candidate proteins detected at significantly higher levels in EV compared with WCL, or compared with EV from normal oral keratinocytes, were identified and confirmed by Wes Simple Western protein analysis...
July 23, 2021: Cancers
#4
REVIEW
Rubén López-Cortés, Benito Blanco Gómez, Sergio Vázquez-Estévez, Daniel Pérez-Fentes, Cristina Núñez
Bladder cancer (BC) is the fifth most common cancer with a high prevalence rate. It is classically classified in two groups, namely non-muscle invasive (NMIBC) and muscle invasive (MIBC). NMIBC accounts for 75% of cases and has a better prognosis than MIBC. However, 30-50% of the NMIBC patients will show recurrences throughout their lives, and about 10-20% of them will progress to MIBC, with frequent metastasis and a reduced survival rate. The diagnosis of bladder cancer is confirmed by direct visualization of the tumour and other mucosal abnormalities with endoscopic excision using cystoscopy and transurethral resection of the bladder (TURBT)...
September 15, 2021: Journal of Proteomics
#5
JOURNAL ARTICLE
Xiao-Qing Ding, Zhe-Ying Wang, Di Xia, Rui-Xian Wang, Xiao-Rong Pan, Jian-Hua Tong
Background: Clinical management of metastatic gastric cancer (mGC) remains a major challenge due to a lack of specific biomarkers and effective therapeutic targets. Recently, accumulating evidence has suggested that exosomes play an essential role in cancer metastasis and can be an excellent reservoir of novel biomarkers and candidate therapeutic targets for cancer. Therefore, in this study, we aimed to reveal the proteomic profile of mGC-derived exosomes. Methods: Exosomes were isolated from pooled serum samples of 20 mGC patients and 40 healthy controls (HC) by ultracentrifugation...
2020: Frontiers in Oncology
#6
JOURNAL ARTICLE
Jiajia Shen, Jing Zhai, Xinqian Wu, Guiping Xie, Lizong Shen
Searching for the novel tumour biomarkers is pressing for gastric cancer diagnostication and prognostication. The serum specimens from patients diagnosed with locally advanced gastric carcinoma before operation and 4 week after surgery were collected, respectively, and serum proteome profiling was conducted by liquid chromatography-mass spectrometry (MS)/MS. Fifty-five proteins were identified to be up-regulated and 16 proteins were down-regulated, and these differentially expressed proteins participated in various biological processes...
June 2020: Journal of Cellular and Molecular Medicine
#7
JOURNAL ARTICLE
Bruna Calsina, Luis Jaime Castro-Vega, Rafael Torres-Pérez, Lucía Inglada-Pérez, Maria Currás-Freixes, Juan María Roldán-Romero, Veronika Mancikova, Rocío Letón, Laura Remacha, María Santos, Nelly Burnichon, Charlotte Lussey-Lepoutre, Elena Rapizzi, Osvaldo Graña, Cristina Álvarez-Escolá, Aguirre A de Cubas, Javier Lanillos, Alfonso Cordero-Barreal, Ángel M Martínez-Montes, Alexandre Bellucci, Laurence Amar, Fabio Luiz Fernandes-Rosa, María Calatayud, Javier Aller, Cristina Lamas, Júlia Sastre-Marcos, Letizia Canu, Esther Korpershoek, Henri J Timmers, Jacques Wm Lenders, Felix Beuschlein, Martin Fassnacht-Capeller, Graeme Eisenhofer, Massimo Mannelli, Fátima Al-Shahrour, Judith Favier, Cristina Rodríguez-Antona, Alberto Cascón, Cristina Montero-Conde, Anne-Paule Gimenez-Roqueplo, Mercedes Robledo
Rationale : Pheochromocytomas and paragangliomas (PPGLs) are rare neuroendocrine tumors that present variable outcomes. To date, no effective therapies or reliable prognostic markers are available for patients who develop metastatic PPGL (mPPGL). Our aim was to discover robust prognostic markers validated through in vitro models, and define specific therapeutic options according to tumor genomic features. Methods : We analyzed three PPGL miRNome datasets (n=443), validated candidate markers and assessed them in serum samples (n=36) to find a metastatic miRNA signature...
2019: Theranostics
#8
JOURNAL ARTICLE
Cuiping Li, Yang Zhou, Junjun Liu, Xiaoping Su, Hao Qin, Suhua Huang, Xuanping Huang, Nuo Zhou
BACKGROUND: Blood contains exosomes that are related to tumor cells. Those exosomes can regulate communication between cells and have a great influence on a variety of tumor-associated proceedings through their target cells. Therefore, serum exosomes (SE) were supposed to play a crucial role in cancer development. METHODS: This study presented a quantitative proteomics analysis to identify the protein content in SEs including 30 subjects from oral squamous cell carcinoma (OSCC) patients with lymph node metastasis (LNM), OSCC patients with no LNM (NLNM), and healthy controls (HC)...
October 2019: Cancer Epidemiology, Biomarkers & Prevention
#9
JOURNAL ARTICLE
Eleni Zografos, Athanasios K Anagnostopoulos, Aggeliki Papadopoulou, Evangelia Legaki, Flora Zagouri, Evangelos Marinos, George T Tsangaris, Maria Gazouli
BACKGROUND: To date, the elucidation of serum protein alterations in male breast cancer (MBC) has not been extensively studied, due to the rarity of the disease. MATERIALS AND METHODS: In the present work, two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) were employed to detect differences in serum protein expression between patients with MBC and healthy controls. RESULTS: A panel of differentially expressed serum proteins was identified, including proteins involved in the regulation of the cell cycle [e...
March 2019: Cancer Genomics & Proteomics
#10
JOURNAL ARTICLE
Hailong Fu, Huan Yang, Xu Zhang, Bo Wang, Jiahui Mao, Xia Li, Mei Wang, Bin Zhang, Zixuan Sun, Hui Qian, Wenrong Xu
BACKGROUND: Exosomes are critically involved in cancer development and progression. The exosomal contents have been suggested as ideal cancer biomarkers. In this study, we investigated the expression of exosomal proteins in the serum of gastric cancer patients and their roles in gastric cancer. METHODS: The proteomic profile of exosomes from the serum of gastric cancer patients was detected by using LC-MS/MS. The expression of TRIM3 in exosomes from the serum of gastric cancer patients and healthy controls was assessed by ELISA and western blot...
July 21, 2018: Journal of Experimental & Clinical Cancer Research: CR
#11
JOURNAL ARTICLE
Zarina S Lalmahomed, Mirelle Ee Bröker, Nick A van Huizen, Robert R J Coebergh van den Braak, Lennard Jm Dekker, Dimitris Rizopoulos, Cornelis Verhoef, Ewout W Steyerberg, Theo M Luider, Jan Nm IJzermans
The clinical efficacy of carcinoembryonic antigen (CEA) as a marker of colorectal liver metastasis is limited, motivating a search for new biomarkers. Recently, urine proteomic analysis revealed AGPP(-OH)GEAGKP(-OH)GEQGVP(-OH)GDLGAP(-OH)GP (AGP), a promising peptide for this application. This study aimed to determine whether combining urine AGP testing with serum CEA analyses improves the sensitivity of detecting colorectal liver metastases. Urine samples from 100 patients with CRLM were collected prospectively and compared to three control groups: healthy kidney donors, patients who were relapse-free for 24 months after curative CRLM surgery, and primary colorectal cancer patients...
2016: American Journal of Cancer Research
#12
JOURNAL ARTICLE
Hui Zhang, Hao Li, Fei Guo, Da Zhang, Heying Yang, Jiaxiang Wang
OBJECTIVE: To screen and identify the serum specific protein markers of patients with gastric cancer by proteomics technology, and to provide more comprehensive serum protein fingerprint model for the early diagnosis of gastric cancer. METHODS: Preoperative and postoperative blood samples were collected from 60 gastric cancer patients. Mass spectrometry (SELDI-TOF-MS) technology was used to detect and screen serum specific proteins in gastric cancer patients(preoperative group, postoperative group, metastasis group), and the result was compared with normal control group...
March 2016: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
#13
JOURNAL ARTICLE
Bashar Zeidan, Thomas R Jackson, Samantha E T Larkin, Ramsey I Cutress, Gary R Coulton, Margaret Ashton-Key, Nick Murray, Graham Packham, Vassilis Gorgoulis, Spiros D Garbis, Paul A Townsend
Breast cancers are the most common cancer-affecting women; critically the identification of novel biomarkers for improving early detection, stratification and differentiation from benign tumours is important for the reduction of morbidity and mortality.To identify and functionally characterise potential biomarkers, we used mass spectrometry (MS) to analyse serum samples representing control, benign breast disease (BBD) and invasive breast cancer (IDC) patients. Complementary and multidimensional proteomic approaches were used to identify and validate novel serum markers...
August 28, 2015: Oncotarget
#14
JOURNAL ARTICLE
Betul Celik, Arzu Didem Yalcin, Gizem Esra Genc, Saadet Gumuslu
PURPOSE: The purpose of this study was to evaluate the soluble Apo-2L (sApo-2L) levels in the ascitic fluid and to study its potential in detecting malignant ascites and soluble CD200 (sCD200,sOX-2) levels so as to predict its clinical usage for detecting stage 4 metastatic endometrial, ovarian and breast cancer in serum samples. METHODS: Ascitic fluid from 53 and blood from 25 subjects without known malignancy on admission were collected. There were 14 breast cancer (BC), 17 ovarian cancer (OC) and 19 endometrial cancer (EC) patients diagnosed later on...
January 2015: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
#15
JOURNAL ARTICLE
Ming Guan, Xiaofan Chen, Yingyu Ma, Lihua Tang, Lei Guan, Xuefeng Ren, Bo Yu, Wei Zhang, Bing Su
Metastatic melanoma, the primary cause of skin cancer-related death, warrants new diagnostic and therapeutic approaches that target the regulatory machinery at molecular level. The heterogeneity and complexity of melanoma result in the difficulty to find biomarkers and targets for early detection and treatment. Here, we investigated metastasis-associated proteins by comparing the proteomic profiles of primary cutaneous melanomas to their matched lymph node metastases, which minimizes heterogeneity among samples from different patients...
April 2015: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
#16
COMPARATIVE STUDY
Sujatha Bhat, Vasudevan Bhaskaran Kartha, Lavanya Rai, Santhosh Chidangil
Cervical cancer, the second most common cancer in women, progresses silently over long periods before producing any clinical manifestation. Research in early detection of this condition using proteomic techniques is of very recent origin. We used high-performance liquid chromatography combined with laser-induced fluorescence method in our lab to record the protein profiles of tissue homogenate, cell lysate and serum samples of normal and different stages of malignant conditions of the cervix. Information on protein markers in the protein profiles was derived using various data processing methods including curve resolution...
January 2015: Journal of Chromatographic Science
#17
JOURNAL ARTICLE
J Shi, J Liu, L Liao, Y Guo, H Wang, W Hu, T Hu
AIM: Detection of novel tumor biomarker will aid in diagnosis of early-stage small cell lung cancer (SCLC). The purpose of this study was to identify novel tumor biomarker in serum from patients with SCLC using a proteomics-based approach. METHODS: Sera were analyzed before the initiation of chemotherapy. Serum proteins of SCLC patients and healthy controls were collected and separated by 2-D fluorescence differential gel electrophoresis (2-D DIGE). Positive spots were analyzed by LC-MS/MS...
April 2014: Minerva Medica
#18
JOURNAL ARTICLE
Sho Minami, Ryo Nagashio, Junpei Ueda, Kazumasa Matsumoto, Naoki Goshima, Manabu Hattori, Kazuo Hachimura, Masatsugu Iwamura, Yuichi Sato
Secreted proteins play essential roles in the process of tumorigenesis, and the analysis of tumor-secreted proteins has been suggested as a promising strategy for identifying cancer biomarkers. In this study, we performed proteomic analysis to identify proteins secreted from bladder cancer cell lines that are recognized by autoantibodies in sera from patients with bladder cancer. In addition,autoantibodies against the identified proteins were validated using a dot-blot array with sera from patients with bladder cancer and normal controls...
2014: Biomedical Research
#19
RANDOMIZED CONTROLLED TRIAL
Xia Zou, Bo Feng, Taotao Dong, Guoquan Yan, Binbin Tan, Hao Shen, Ao Huang, Xiu Zhang, Menghui Zhang, Pengyuan Yang, Minhua Zheng, Yan Zhang
Colorectal cancer (CRC) is one of the most prevalent cancers worldwide. The discovery of non-invasive biomarker candidates for diagnosis and prognosis is important for the management of CRC. In this study, we performed proteomic profiling of serum from patients with different stages of CRC using a 2D-LC-MS/MS based approach combined with the APEX quantitative method. 917 proteins were identified and 93 were differentially expressed in normal and three patient groups (stages I, II and III). These proteins were predominantly involved in cell adhesion, immune response, coagulation process and metabolism...
December 6, 2013: Journal of Proteomics
#20
JOURNAL ARTICLE
Qing-Lian Wan, Xiang-Sheng Hou, Guang Zhao
Esophageal cancer (EC) is one of the most common malignant tumors, and the incidence of esophageal squamous cell carcinoma (ESCC) is highest in China. Early diagnosis and effective monitoring are keys to comprehensive treatment and discovering tumor metastases and recurrence in time. The aim of this study was to confirm serum peptidome pattern utility for diagnosis of ESCC, and assessment of operation success, postoperative chemotherapy results, tumor metastasis and recurrence. Serum samples were collected from 61 patients treated with surgery and chemotherapy and 20 healthy individuals...
2013: Asian Pacific Journal of Cancer Prevention: APJCP
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