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Swetlana Rot, Helge Taubert, Matthias Bache, Thomas Greither, Peter Würl, Hans-Jürgen Holzhausen, Alexander W Eckert, Dirk Vordermark, Matthias Kappler
In various tumors, the hypoxia inducible factor-1α ( HIF-1α ) and the epidermal growth factor-receptor ( EGFR ) have an impact on survival. Nevertheless, the prognostic impact of both markers for soft tissue sarcoma (STS) is not well studied. We examined 114 frozen tumor samples from adult soft tissue sarcoma patients and 19 frozen normal tissue samples. The mRNA levels of HIF-1α, EGFR, and the reference gene hypoxanthine phosphoribosyltransferase (HPRT) were quantified using a multiplex qPCR technique. In addition, levels of EGFR or HIF-1α protein were determined from 74 corresponding protein samples using ELISA techniques...
December 3, 2018: International Journal of Molecular Sciences
Efseveia Frakolaki, Panagiota Kaimou, Maria Moraiti, Katerina I Kalliampakou, Kalliopi Karampetsou, Eleni Dotsika, Panagiotis Liakos, Dido Vassilacopoulou, Penelope Mavromara, Ralf Bartenschlager, Niki Vassilaki
Low oxygen tension exerts a profound effect on the replication of several DNA and RNA viruses. In vitro propagation of Dengue virus (DENV) has been conventionally studied under atmospheric oxygen levels despite that in vivo, the tissue microenvironment is hypoxic. Here, we compared the efficiency of DENV replication in liver cells, monocytes, and epithelial cells under hypoxic and normoxic conditions, investigated the ability of DENV to induce a hypoxia response and metabolic reprogramming and determined the underlying molecular mechanism...
December 1, 2018: Cells
Weixiu Ji, Linjia Wang, Shiyi He, Lu Yan, Tieying Li, Jianxiong Wang, Ah-Ng Tony Kong, Siwang Yu, Ying Zhang
BACKGROUND: Hypoxia training enhances the endurance capacity of athletes. This response may in part be attributed to the hypoxia-induced increase in antioxidant capacity in skeletal muscles. Nuclear factor erythroid 2-related factor 2 (Nrf2), a key transcription factor which regulates the expression of genes via binding to the antioxidant-response element (ARE) of these genes, plays a crucial role in stimulating the body's defense system and potentially responds to hypoxia. Meanwhile, hypoxia-inducible factor-1α (HIF-1α) is an important player in protecting cells from hypoxic stress...
2018: PloS One
Guliang Yang, Chia-Che Chang, Yiwen Yang, Li Yuan, Leishiyuan Xu, Chi-Tang Ho, Shiming Li
Rheumatoid arthritis (RA) is a systemic autoimmune disease primarily affecting joints and is featured by chronic synovial inflammation and angiogenesis. We employed a bovine type-II collagen (BIIC)-induced Sprague-Dawley rat arthritis model and an in vitro RA model based on interleukin (IL)-1β-stimulated rat synovial cells (RSC-364) to explore the preventive effect of resveratrol on RA and the underlying mechanisms. We found that resveratrol ameliorated BIIC-elicited synovitis and RA-related pathological hallmarks such as inflammatory cell infiltration and angiogenesis in the synovial tissue...
December 4, 2018: Journal of Agricultural and Food Chemistry
Constantin Römmelt, Thomas Munsch, Andreas Drynda, Volkmar Lessmann, Christoph H Lohmann, Jessica Bertrand
The reasons for the high number of loosened metal-on-metal (MoM) hip implants are still not fully understood. Hypoxia-inducible factor 1 (HIF-1) mediated signaling pathways, which normally modulate tissue metabolism under hypoxic circumstances, could be triggered by metallic wear debris and influence bone metabolism favoring osteolysis. This may lead to early loosening of the orthopedic implants. Immunhistochemical staining of periprosthetic tissues of failed artificial hip implants showed that the concentration of HIF-1α in the surrounding tissues of failed MoM hip implants was significantly higher in comparison to failed metal-on-polyethylene (MoP) hip implants and osteoarthritic tissues...
December 3, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Iris E M de Jong, Alix P M Matton, Jasper B van Praagh, Wouter T van Haaften, Janneke Wiersema-Buist, Louise A van Wijk, Dorenda Oosterhuis, Raditya Iswandana, Su Suriguga, Diletta Overi, Ton Lisman, Guido Carpino, Annette S H Gouw, Peter Olinga, Eugenio Gaudio, Robert J Porte
Peribiliary glands (PBG) are a source of stem/progenitor cells organized in a cellular network encircling large bile ducts. Severe cholangiopathy with loss of luminal biliary epithelium has been proposed to activate PBG, resulting in cell proliferation and differentiation to restore biliary epithelial integrity. However, formal evidence for this concept in human livers is lacking. We, therefore, developed a novel ex vivo model using precision-cut slices of extrahepatic human bile ducts obtained from discarded donor livers, providing an intact anatomical organization of cell structures, to study spatiotemporal differentiation and migration of PBG cells after severe biliary injury...
December 2, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
Shanshan Lin, Bo Zhu, Guozhi Huang, Qing Zeng, Chuhuai Wang
Studies have demonstrated that mesenchymal stem cells (MSCs) can promote tumor growth, and MSC microvesicles (MVs) are very important in the tumor microenvironment and information transfer between cells during tumorigenesis and development. However, the potential effects and mechanisms of MSC-MVs on tumor growth are still controversial. Here in this study, we investigated the roles and effects of human bone marrow MSC-MVs (hBMSC-MVs) on human osteosarcoma (U2OS) cell growth under hypoxia in vitro and in vivo...
November 30, 2018: Human Cell
Xinrui Zhao, Lidong Liu, Rui Li, Xuan Wei, Wenqing Luan, Peishu Liu, Jing Zhao
BACKGROUND Hypoxia induces cell apoptosis in the uterosacral ligaments of patients with pelvic organ prolapse by upregulation of hypoxia-inducible factor-1α (HIF-1α). This study aimed to investigate the effects of HIF-1α on human uterosacral ligament fibroblasts (hUSLFs) following treatment with the chemical inducer of hypoxia, cobalt chloride (CoCl2), and to explore the underlying mechanisms. MATERIAL AND METHODS Ten women who underwent hysterectomy for benign disease provided uterosacral ligament tissue for cell extraction...
December 3, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Sha Li, Shuren Li
OBJECTIVE: To evaluate the effects of transplantation of hypoxia-inducible factor-1α (HIF-1α) gene-modified cardiac stem cells (CSCs) on the cardiac function of heart failure rats after myocardial infarction (MI). METHODS: Twenty-four Sprague-Dawley rats were randomly divided into three groups: HIF-1α-modified CSCs group, single CSCs group, and model group. The model of heart failure after MI was established by thoracotomy-left anterior descending coronary artery ligation, followed by injection of modified CSCs, single CSCs, and PBS, respectively, 2 weeks later...
December 2018: Anatolian Journal of Cardiology
M Mansour, J Degheili, I Khalifeh, H Tamim, R F Jaafar, Y El-Hout
BACKGROUND: Testicular torsion is a surgical emergency mainly affecting adolescent boys, with a relatively high rate of missed torsion and testicular loss secondary to delay in prompt diagnosis and surgical intervention. With ischemic reperfusion injury as its underlying culprit, testicular torsion may respond favorably to remote ischemic conditioning (RIC) where a non-privileged site (e.g. limb) is concurrently rendered ischemic to divert the cascade of reperfusion injury from the privileged organ (e...
September 24, 2018: Journal of Pediatric Urology
Jun-Qiu Chen, Liang-Hu Huang
Mesenchymal stem cells (MSC) have the potential of multi-directional differentiation, and can recruit endothelial cells, promote their proliferation, migration and angiogenesis, improve blood perfusion and oxygen suppliment, and repair damaged tissue. Exosome secreted by MSC contain mother cell-specific proteins, lipids and nucleic acids, and acts as signaling molecule, playing an important role in cell communication, thereby altering target cell function. In this review, the biological characteristics of MSC and its exosome, the mechanism of promoting vascular regeneration in patients with ischemic diseases, and the mechanism of hypoxia-inducible factor-1α(HIF-1α) in the vascular ischemia of ischemic diseases are all summarized briefly...
December 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Zhonghua Jiang, Hongmei Yang, Xiuyun Zhang, Zhendong Wang, Rong Rong, Xiaohong Wang
Histone deacetylase 1 (HDAC1) has been shown to be closely associated with tumor development. We investigated its effects on survival and biological behavior in gastric cancer (GC). HDAC1 expression and glycolysis activity were analyzed in a cohort of 252 samples of primary GC tumors and in vitro study. High HDAC1 (HDAC1High ) staining was seen in 60.7% patients with GCs, which was significantly greater than was seen in normal epithelial cells (19.4%; P<.005). HDAC1High expression was associated with larger tumor size (P=...
November 27, 2018: Human Pathology
Banafshe Esmaeilzade, Tayebe Artimani, Iraj Amiri, Rezvan Najafi, Siamak Shahidi, Marie Sabec, Parviz Farzadinia, Mohammadali Zare, Maria Zahiri, Sara Soleimani Asl
Mesenchymal stem cell (MSC) transplantation therapy has been proposed as a promising approach for the treatment of neurodegenerative disease. Chemical and pharmacological preconditioning before transplantation could optimize the therapeutic properties of transplanted MSCs. In this study, we hypothesized that preconditioning treatment with a prolyl hydroxylase inhibitor, dimethyloxalylglycine (DMOG), will increase MSC efficacy and paracrine effects in an amyloid-β (Aβ)-injected Alzheimer rat model. MSCs were incubated in different concentrations of DMOG for 24 h...
November 27, 2018: Physiology & Behavior
Ching-Ching Yeh, Jun-Liang Luo, Nam Nhut Phan, Yi-Chun Cheng, Lu-Ping Chow, Mong-Hsun Tsai, Eric Y Chuang, Liang-Chuan Lai
Hypoxia plays a crucial role in the aggressiveness of solid tumors by driving multiple signaling pathways. Recently, long non-coding RNA (lncRNA) has been reported to promote or inhibit tumor aggressiveness by regulating gene expression. Previous studies in our laboratory found that the lncRNA NDRG1-OT1 is significantly up-regulated under hypoxia and inhibits its target gene NDRG1 at both the mRNA and protein levels. At the protein level, NDRG1-OT1 increases NDRG1 degradation via ubiquitin-mediated proteolysis...
November 30, 2018: RNA Biology
Shulin Li, Yuan Zhao, Zhuojun Wang, Jia Wang, Caixia Liu, Dong Sun
Amniotic fluid-derived stem cells (AFSCs), which exhibit both embryonic and mesenchymal stem cell characteristics, have been shown to mitigate the degree of renal interstitial fibrosis. The aim of the present study was to determine whether transplantation of glial cell line-derived neurotrophic factor (GDNF)-modified AFSCs is more useful than transplantation of unmodified AFSCs for the treatment of renal interstitial fibrosis. Mice were randomly assigned to a sham-operation group (sham), a unilateral ureteral obstruction (UUO)-saline solution group (UUO), an AFSC transplantation group (AFSC) and a GDNF-modified AFSC transplantation group (GDNF-AFSC) and sacrificed at days 3 and 7 post-surgery (six in each group)...
November 30, 2018: Cell Transplantation
Maya Cohen, Sharon Amir, Maya Golan, Yinon Ben-Neriah, Nicola J Mabjeesh
The substantial availability of hypoxia-inducible factor 1 (HIF-1) for pathophysiological states, such as malignancies and ischemia, is primarily regulated post-translationally through the ubiquitin proteolytic system. The balance between degradation and stabilization of HIF-1α protein is determined by specific E3 ligases. In our search for new E3 ligases that might affect HIF-1α protein expression, we studied the effects of beta-transducin repeat-containing protein (β-TrCP) on the hypoxic pathway in cancer cells...
November 28, 2018: Prostate
Hongyu Tan, Liang Zhao
Increasing evidence shows that the long noncoding RNA nuclear enriched abundant transcript 1 (NEAT1) plays important roles in tumor progression. However, the function and the underlying mechanism of NEAT1 in osteosarcoma (OS) remain unclear. In the present study, we found that NEAT1 expression was significantly upregulated in OS tissues and cell lines. High NEAT1 expression was closely associated with advanced clinicopathologic features and poor overall survival of patients with OS. Using in vitro function assay, we found that NEAT1 could promote the proliferation, invasion, and epithelial-mesenchymal transition (EMT) process of OS cells...
November 28, 2018: Journal of Cellular Biochemistry
(no author information available yet)
OBJECTIVEThe relationship between intraplaque hypoxia and intraplaque hemorrhage (IPH) has been reported, but the details remain obscure. In this study, the authors aimed to clarify the relationship among intraplaque hypoxia, endothelial progenitor cells (EPCs), and neovascularization, which causes IPH. The histological findings of specimens obtained from carotid endarterectomy were assessed.METHODSThis study included 49 patients who underwent carotid endarterectomy. Magnetic resonance plaque imaging was performed to analyze the components of the carotid plaques, and surgical specimens were subjected to immunohistochemical analysis...
October 1, 2018: Journal of Neurosurgery
Yuan-Yuan Li, Tong Hao, Yun Lu, Yue-Hua Liu
PURPOSE: To investigate the role of 17β-estradiol (E2) and resveratrol dimer (RD) on HIF-1α and the underlying mechanism. METHODS: Mice genioglossus myoblasts were isolated and cultured, and the estrogen receptor-α (ERα) shRNA lentivirus was used for gene knockdown. Cells in different groups were treated with different agents (E2, or RD, or E2 and LY294002), then incubated in normoxia or hypoxia for 24 h, the expressions of HIF-1α, ERα, ERβ, total-Akt and phospho-Akt were detected using qRT-PCR and Western blot...
August 2018: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
Sanuo Cheng, Xingping Zhang, Yali Xu, Xiaobo Dai, Jiachu Li, Tao Zhang, Xiaopin Chen
Tumor angiogenesis plays a critical role in hepatocellular carcinoma (HCC) development and progression, but its mechanism is unclear. Krüppel-like factor 8 (KLF8) is a transcription factor that plays an important role in HCC progression. Here, we investigated the role of KLF8 in angiogenesis in HCC and its possible mechanism. Immunohistochemistry, quantitative RT-PCR, western blotting, promoter reporter assays, chromatin immunoprecipitation (ChIP), and chicken chorioallantoic membrane (CAM) and nude mouse tumor models were used to show that the mRNA and protein expression levels of KLF8 and VEGFA are highly correlated in HCC tissue samples...
November 27, 2018: Scientific Reports
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