keyword
https://read.qxmd.com/read/38532782/identification-of-a-novel-non-v600e-braf-mutation-in-papillary-thyroid-cancer
#1
Marco Capezzone, Maja Rossi, Elisabetta Macerola, Silvia Cantara, Francesco Pepe, Eugenia Maria Morabito, Gilda Dalmazio, Sara Bardi, Agostino Ognibene, Massimo Alessandri, Gabriele Materazzi, Luigi De Napoli, Michele Cirianni, Liborio Torregrossa
Papillary thyroid cancer (PTC) is a common endocrine malignancy, and its incidence is reported to be constantly increasing. BRAF mutation is detected in approximately 44% of PTCs, and the most common BRAF mutation is thymine (T) to adenine (A) missense mutation in nucleotide 1796 (T1796A, V600E). Although BRAFV600E represents 95% of all BRAF mutations, uncommon BRAF mutations have been reported in thyroid carcinomas and represent an alternative mechanism of BRAF activation with unclear clinical significance...
2024: Case Reports in Endocrinology
https://read.qxmd.com/read/38529489/ehmt2-inactivation-in-pancreatic-epithelial-cells-shapes-the-transcriptional-landscape-and-inflammation-response-of-the-whole-pancreas
#2
Gareth Pollin, Angela J Mathison, Thiago M de Assuncao, Anju Thomas, Lida Zeighami, Ann Salmonson, Hongfei Liu, Guillermo Urrutia, Pallavi Vankayala, Stephen J Pandol, Michael T Zimmermann, Juan Iovanna, Victor X Jin, Raul Urrutia, Gwen Lomberk
The Euchromatic Histone Methyl Transferase Protein 2 (EHMT2), also known as G9a, deposits transcriptionally repressive chromatin marks that play pivotal roles in the maturation and homeostasis of multiple organs. Recently, we have shown that EHMT2 inactivation alters growth and immune gene expression networks, antagonizing KRAS-mediated pancreatic cancer initiation and promotion. Here, we elucidate the essential role of EHMT2 in maintaining a transcriptional landscape that protects organs from inflammation...
March 16, 2024: bioRxiv
https://read.qxmd.com/read/38525710/-candida-variety-in-the-oral-cavity-of-mexican-subjects-with-type-2-diabetes-mellitus-and-tlr2-gene-expression
#3
JOURNAL ARTICLE
Nadia Mabel Pérez-Vielma, Modesto Gómez-López, María de Los Ángeles Martínez-Godínez, Ana Laura Luna-Torres, Aarón Domínguez López, Ángel Miliar-García
BACKGROUND: The aim was to diagnose Candida in the oral cavity of subjects with type 2 diabetes mellitus (T2DM) using a genotyping technique and compare the results with those from conventional diagnosis by Papanicolaou (Pap) staining. METHODS: Palatal mucosa smears were performed on 18 dental care patients diagnosed with T2DM and grade I, II, and III prosthetic stomatitis who met the inclusion criteria; 18 healthy control subjects were also included in the study...
February 27, 2024: Clinics and Practice
https://read.qxmd.com/read/38524273/dna-methylation-as-a-triage-tool-for-cervical-cancer-screening-a-meeting-report
#4
REVIEW
F Ricardo Burdier, Dur-E-Nayab Waheed, Belinda Nedjai, Renske D M Steenbergen, Mario Poljak, Marc Baay, Alex Vorsters, Severien Van Keer
INTRODUCTION: DNA methylation is proposed as a novel biomarker able to monitor molecular events in human papillomavirus (HPV) infection pathophysiology, enabling the distinction between HPV-induced lesions with regression potential from those that may progress to HPV-related cancer. METHODS: This meeting report summarises the presentations and expert discussions during the HPV Prevention and Control Board-focused topic technical meeting on DNA methylation validation in clinician-collected and self-collected samples, novel DNA methylation markers discovery, implementation in cervical cancer screening programs, and their potential in women living with human immunodeficiency virus (HIV)...
May 2024: Preventive Medicine Reports
https://read.qxmd.com/read/38520219/comprehensive-evaluation-of-cytomorphologic-histologic-and-molecular-features-of-dicer1-altered-thyroid-lesions-on-fna-a-multipractice-experience
#5
JOURNAL ARTICLE
Krisztina Lengyel, Daniel J Lubin, Wen-Yu Hsiao, Sam Sirotnikov, Guangju Luo, James W Roberts, Qiuying Shi, Kelly Magliocca, Melinda M Lewis, Donald L Sears, Ghulam Ilyas, Beverly B Rogers, Kartik Viswanathan
BACKGROUND: DICER1 mutations, though infrequent, are encountered on preoperative molecular testing of indeterminate adult and pediatric thyroid fine-needle aspiration (FNA) specimens. Yet, published cytomorphologic features of DICER1-altered thyroid lesions are limited. Cytomorphological features of DICER1-altered thyroid lesions were examined in a multipractice FNA cohort with clinical, radiological, and histologic data. METHODS: The cohort comprised 18 DICER1-altered thyroid FNAs, with 14 having slides available and eight having corresponding surgical resections...
March 23, 2024: Cancer Cytopathology
https://read.qxmd.com/read/38519839/minimally-invasive-biopsy-based-diagnostics-in-support-of-precision-cancer-medicine
#6
REVIEW
Bo Franzén, Gert Auer, Rolf Lewensohn
Precision cancer medicine (PCM) to support the treatment of solid tumors requires minimally invasive diagnostics. Here, we describe the development of fine-needle aspiration biopsy-based (FNA) molecular cytology which will be increasingly important in diagnostics and adaptive treatment. We provide support for FNA-based molecular cytology having a significant potential to replace core needle biopsy (CNB) as a patient-friendly potent technique for tumor sampling for various tumor types. This is not only because CNB is a more traumatic procedure and may be associated with more complications compared to FNA-based sampling, but also due to the recently developed molecular methods used with FNA...
March 22, 2024: Molecular Oncology
https://read.qxmd.com/read/38519805/highly-sensitive-detection-of-melanin-in-melanomas-using-multi-harmonic-low-frequency-epr
#7
JOURNAL ARTICLE
Mohammad Wehbi, Lionel Mignion, Nicolas Joudiou, Evelyne Harkemanne, Bernard Gallez
PURPOSE: Low frequency EPR can noninvasively detect endogenous free radical melanin in melanocytic skin lesions and could potentially discriminate between benign atypical nevi and malignant melanoma lesions. We recently succeeded in demonstrating the ability of clinical EPR to noninvasively detect the endogenous melanin free radical in skin lesions of patients. However, the signal-to-noise ratio (SNR) was extremely low warranting further research to boost the sensitivity of detection...
March 22, 2024: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://read.qxmd.com/read/38519095/synaptic-electrophysiology-of-the-drosophila-neuromuscular-junction
#8
JOURNAL ARTICLE
Bing Zhang, Bryan Stewart
Chemical synaptic transmission is an important means of neuronal communication in the nervous system. Upon the arrival of an action potential, the nerve terminal experiences an influx of calcium ions, which in turn trigger the exocytosis of synaptic vesicles (SVs) and the release of neurotransmitters into the synaptic cleft. Transmitters elicit synaptic responses in the postsynaptic cell by binding to and activating specific receptors. This is followed by the recycling of SVs at presynaptic terminals. The Drosophila larval neuromuscular junction (NMJ) shares many structural and functional similarities to synapses in other animals, including humans...
March 22, 2024: Cold Spring Harbor Protocols
https://read.qxmd.com/read/38519094/focal-recording-of-synaptic-currents-from-single-boutons-at-the-drosophila-larval-neuromuscular-junction
#9
JOURNAL ARTICLE
Bing Zhang, Bryan Stewart
Focal recording is an extracellular method for studying synaptic transmission at the Drosophila larval neuromuscular junction (NMJ) designed for the study of synaptic activity of one or a few synaptic boutons rather than the ensemble activity of all the boutons as occurs with intracellular recording or two-electrode voltage-clamp. This is a useful technique for investigating the properties of different motor neurons that innervate the same muscle, applying statistical analysis to discrete synaptic events, and investigating the heterogeneity of synaptic release properties among boutons...
March 22, 2024: Cold Spring Harbor Protocols
https://read.qxmd.com/read/38519093/recording-from-drosophila-larval-body-wall-muscles-passive-membrane-properties-and-basic-features-of-synaptic-transmission
#10
JOURNAL ARTICLE
Bing Zhang, Bryan Stewart
The Drosophila larval body wall muscle preparation was first used for electrophysiological analysis in the 1970s. This preparation has become the "gold standard" for studying neuronal excitability as well as synaptic transmission. Here, we first describe the steps for performing intracellular recording from fly larval body wall muscles and then explain how to record and analyze spontaneous and evoked synaptic potentials. Methods used include larval dissection (filleting), identification of muscle fibers and their innervating nerves, the use of the micromanipulator and microelectrode in penetrating the muscle membrane, and nerve stimulation to evoke synaptic potentials...
March 22, 2024: Cold Spring Harbor Protocols
https://read.qxmd.com/read/38519092/voltage-clamp-analysis-of-synaptic-transmission-at-the-drosophila-larval-neuromuscular-junction
#11
JOURNAL ARTICLE
Bing Zhang, Bryan Stewart
Although it is particularly valuable in revealing membrane potential changes, intracellular recording has a number of limitations. Primarily, it does not offer information on the kinetics of membrane currents associated with ion channels or synaptic receptors responsible for the potential change. Furthermore, the resting potential of the Drosophila body wall muscle varies naturally such that the driving force also varies considerably, making it difficult to accurately compare the amplitude of miniature synaptic potentials (minis) or evoked excitatory junction potentials (EJPs)...
March 22, 2024: Cold Spring Harbor Protocols
https://read.qxmd.com/read/38519091/fabrication-of-microelectrodes-suction-electrodes-and-focal-electrodes-for-electrophysiological-recording-in-drosophila
#12
JOURNAL ARTICLE
Bing Zhang, Bryan Stewart
Electrophysiological recording is a group of techniques used to record electrical field potentials generated by cells. These techniques rely on several types of electrodes, which can be manufactured in the laboratory. In intracellular recording, glass microelectrodes are used to pierce the cell membrane, and then to measure the electrical potential difference between the inside and the outside of the cell. Another technique, called loose patch or focal recording, is similar to intracellular recording but the electrode tip does not pierce into the cell membrane...
March 22, 2024: Cold Spring Harbor Protocols
https://read.qxmd.com/read/38519090/electrophysiological-recording-from-a-model-cell
#13
JOURNAL ARTICLE
Bing Zhang, Bryan Stewart
The muscle cell or neuron membrane is functionally equivalent to a resistor-capacitor (RC) circuit with the membrane resistance and capacitor in parallel. Once inserted inside the membrane, an electrode introduces a serial resistance and small capacitance to the RC circuit. Through a narrow opening at its tip (∼0.1-μm), current can pass through the electrode, into the cell, and back to the outside (ground) across the membrane to complete the circuit. This arrangement enables a voltage difference between the outside and inside of the cell membrane to be recorded...
March 22, 2024: Cold Spring Harbor Protocols
https://read.qxmd.com/read/38516610/beyond-conventional-approaches-enhancing-photodynamic-therapy-for-refractory-feline-sporotrichosis-caused-by-sporothrix-brasiliensis
#14
Mariana Lucy Mesquita Ramos, Vanessa Brito de Souza Rabello, Erica Aparecida Dos Santos Ribeiro da Silva, Maria Cristina da Silva Lourenço, Rodrigo Almeida-Paes, Susana Frases
Male cat, 2 years old, with a refractory infection by Sporothrix brasiliensis, presents a single nodular lesion in the left auricular pavilion. To confirm the diagnosis, cytology, fungal culture, antifungal susceptibility test, molecular analysis, and, to aid in the differential diagnosis, bacterial culture, antibiogram, and histopathology of the lesion were performed. In the absence of therapeutic success with conventional antifungals, photodynamic therapy (PDT) was introduced, demonstrating a satisfactory response in the sixth treatment session...
June 2024: Medical Mycology Case Reports
https://read.qxmd.com/read/38513632/multiple-genomic-landscapes-of-recombination-and-genomic-divergence-in-wild-populations-of-house-mice-the-role-of-chromosomal-fusions-and-prdm9
#15
JOURNAL ARTICLE
Cristina Marín-García, Lucía Álvarez-González, Laia Marín-Gual, Sònia Casillas, Judith Picón, Keren Yam, María Magdalena Garcias-Ramis, Covadonga Vara, Jacint Ventura, Aurora Ruiz-Herrera
Chromosomal fusions represent one of the most common types of chromosomal rearrangements found in nature. Yet, their role in shaping the genomic landscape of recombination and hence genome evolution remains largely unexplored. Here, we take advantage of wild mice populations with chromosomal fusions to evaluate the effect of this type of structural variant on genomic landscapes of recombination and divergence. To this aim, we combined cytological analysis of meiotic crossovers (COs) in primary spermatocytes with inferred analysis of recombination rates based on linkage disequilibrium using single nucleotide polymorphisms...
March 21, 2024: Molecular Biology and Evolution
https://read.qxmd.com/read/38513167/peritoneal-tumor-dna-as-a-prognostic-biomarker-in-gastric-cancer-a-systematic-review-and-meta-analysis
#16
REVIEW
Zexi Allan, Sasha Witts, Darren J Wong, Margaret M Lee, Jeanne Tie, Niall C Tebbutt, Nicholas J Clemons, David S Liu
PURPOSE: Gastric cancers commonly spread to the peritoneum. Its presence significantly alters patient prognosis and treatment-intent; however, current methods of peritoneal staging are inaccurate. Peritoneal tumor DNA (ptDNA) is tumor-derived DNA detectable in peritoneal lavage fluid. ptDNA positivity may indicate peritoneal micrometastasis and may be more sensitive than cytology in staging the peritoneum. In this meta-analysis, we evaluated the prognostic potential of ptDNA in gastric cancer...
March 2024: JCO Precision Oncology
https://read.qxmd.com/read/38512546/passing-the-molecular-imaging-and-biology-torch
#17
EDITORIAL
H Charles Manning
No abstract text is available yet for this article.
March 21, 2024: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
https://read.qxmd.com/read/38507122/a-lightweight-xai-approach-to-cervical-cancer-classification
#18
JOURNAL ARTICLE
Javier Civit-Masot, Francisco Luna-Perejon, Luis Muñoz-Saavedra, Manuel Domínguez-Morales, Anton Civit
Cervical cancer is caused in the vast majority of cases by the human papilloma virus (HPV) through sexual contact and requires a specific molecular-based analysis to be detected. As an HPV vaccine is available, the incidence of cervical cancer is up to ten times higher in areas without adequate healthcare resources. In recent years, liquid cytology has been used to overcome these shortcomings and perform mass screening. In addition, classifiers based on convolutional neural networks can be developed to help pathologists diagnose the disease...
March 20, 2024: Medical & Biological Engineering & Computing
https://read.qxmd.com/read/38506334/regulation-by-distinct-myb-transcription-factors-defines-the-roles-of-oscyp86a9-in-anther-development-and-root-suberin-deposition
#19
JOURNAL ARTICLE
Xiaoyan Huang, Yiqi Li, Zhenyi Chang, Wei Yan, Chunjue Xu, Baolei Zhang, Zhaohuan He, Changjian Wang, Minting Zheng, Zhiai Li, Jixing Xia, Guoliang Li, Xiaoyan Tang, Jianxin Wu
Cytochrome P450 proteins (CYPs) play critical roles in plant development and adaptation to fluctuating environments. Previous reports have shown that CYP86A proteins are involved in the biosynthesis of suberin and cutin in Arabidopsis. However, the functions of these proteins in rice remain obscure. In this study, a rice mutant with incomplete male sterility was identified. Cytological analyses revealed that this mutant was defective in anther development. Cloning of the mutant gene indicated that the responsible mutation was on OsCYP86A9...
March 20, 2024: Plant Journal
https://read.qxmd.com/read/38502277/divergence-of-10-satellite-repeats-in-artemisia-asteraceae-anthemideae-based-on-sequential-fluorescence-in-situ-hybridization-analysis-evidence-for-species-identification-and-evolution
#20
JOURNAL ARTICLE
Yanze He, Jun He, Yong Zhao, Shuangshuang Zhang, Xinyu Rao, Haibin Wang, Zhenxing Wang, Aiping Song, Jiafu Jiang, Sumei Chen, Fadi Chen
Artemisia is a large genus encompassing about 400 diverse species, many of which have considerable medicinal and ecological value. However, complex morphological information and variation in ploidy level and nuclear DNA content have presented challenges for evolution studies of this genus. Consequently, taxonomic inconsistencies within the genus persist, hindering the utilization of such large plant resources. Researchers have utilized satellite DNAs to aid in chromosome identification, species classification, and evolutionary studies due to their significant sequence and copy number variation between species and close relatives...
March 19, 2024: Chromosome Research
keyword
keyword
166766
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.