Gimena Salas, Alen A Litta, Anabela C Medeot, Virginia Schuck, Romina B Andermatten, Gisel S Miszczuk, Nadia Ciriaci, Ma Valeria Razori, Ismael R Barosso, Enrique J Sánchez Pozzi, Marcelo G Roma, Cecilia L Basiglio, Fernando A Crocenzi
The endogenous metabolite of estradiol, estradiol 17β-D-glucuronide (E17G), is considered the main responsible of the intrahepatic cholestasis of pregnancy. E17G alters the activity of canalicular transporters through a signaling pathway-dependent cellular internalization, phenomenon that was attributed to oxidative stress in different cholestatic conditions. However, there are no reports involving oxidative stress in E17G-induced cholestasis, representing this the aim of our work. Using polarized hepatocyte cultures, we showed that antioxidant compounds prevented E17G-induced Mrp2 activity alteration, being this alteration equally prevented by the NADPH oxidase (NOX) inhibitor apocynin...
April 10, 2024: Biochimie