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Liquid Liquid extraction

Yukiko Matsuo, Tsutomu Yoshida, Kazuya Yamashita, Yukitoshi Satoh
BACKGROUND: Liquid-based cytology (LBC) is a useful cytopathological method, and LBC lung adenocarcinoma specimens may be used for genetic analysis in the near future. In the current study, the authors determined whether LBC specimens can be used for epidermal growth factor receptor (EGFR) mutation analysis in human lung adenocarcinoma cell lines. METHODS: Genomic DNA was extracted from 3 lung adenocarcinoma cell lines that were fixed in LBC preservation solution using 2 protocols (one for cultured cells and one for tissues) of a DNA extraction kit...
October 21, 2018: Cancer Cytopathology
Marieke De Boeck, Lien Moreels, Wim Dehaen, Jan Tytgat, Eva Cuypers
The analysis of biological samples, such as whole blood, comes with several sample preparation challenges. Biological matrices often contain a variety of endogenous components that can interfere with the determination of xenobiotics. Especially blood plasma proteins (e.g. serum albumin) are known to interfere with electrospray ionization and result in analyte ion suppression. Sample preparation techniques should guarantee adequate removal of these biomolecules. The current study aims to determine to which extent proteins are removed from whole blood samples, using ionic liquid-based dispersive liquid-liquid microextraction (IL-DLLME)...
October 10, 2018: Journal of Pharmaceutical and Biomedical Analysis
Ranjana, Zulfa Nooreen, Umme Bushra, Jyotshna, Dnyaneshwar U Bawankule, Karuna Shanker, Ateeque Ahmad, Sudeep Tandon
ETHNOPHARMACOLOGICAL RELEVANCE: Tejovati (Zanthoxylum armatum DC; Family- Rutaceae) popularly known as toothache tree is widely distributed in sub-tropical Himalaya region. Traditionally, The Southeast Asian population of Indo-Nepal origin uses it to treat asthma, gout, pain, and inflammation. The Ayurvedic action of the plant includes the balancing of Vata-Kapha in the body. Which lead to various ailments related to the circulation of blood and water, digestion, immunity, and skin. Therefore, in-vitro xanthine oxidase (XO) inhibition potential of the extract could be worth to explore prospect in the prevention/treatment of gouty affections of the joints and other diseases...
October 18, 2018: Journal of Ethnopharmacology
Cheng Cui, Rui Chen, Ji Jiang, Rui Liu, Wei Wang, Qian Zhao, Pei Hu
FLZ is a novel anti-Parkinson's disease candidate drug. The main active metabolite is FLZ O-dealkylation (M1) in preclinical studies. A reliable ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) quantitation method was developed for the simultaneous determination of FLZ and M1 with low limits of quantitation in human plasma (0.1 ng/mL) and urine (0.5 ng/mL). The plasma and urine samples were both purified by full-automatic solid phase extraction (SPE) method with ensured high extraction recovery and little matrix effect for both analytes, and then separated on a BEH C18 column (2...
October 10, 2018: Journal of Pharmaceutical and Biomedical Analysis
Inês L Martins, João Nunes, Catarina Charneira, Judit Morello, Sofia A Pereira, João P Telo, M Matilde Marques, Alexandra M M Antunes
Carbamazepine (CBZ) is one of the most widely used antiepileptic drugs by both adults and children. Despite its widespread use, CBZ is associated with central nervous system toxicity and severe hypersensitivity reactions, which raise concerns about its chronic use. While the precise mechanisms of CBZ-induced adverse events are still unclear, metabolic activation to the epoxide (CBZ-EP) has been thought to play a significant role. This work reports first-hand evidence that CBZ reacts readily with biologically relevant thiyl radicals with no need for bioactivation...
October 17, 2018: Free Radical Biology & Medicine
Adane Tilahun Getachew, Hee Jeong Lee, Yeon Jin Cho, Sol Ji Chae, Byung Soo Chun
Bioactive polysaccharide was extracted from Pacific oyster Crassostrea gigas using subcritical water (SW) and the extraction process was optimize by response surface methodology (RSM). The optimum condition was found to be temperature 125.01 °C, extraction time 14.93 min, and liquid to solid ratio of 44.69:1 (ml:g). At this condition, the yield of the C. gigas polysaccharides (CGPs) was found to be 18.66%. The polysaccharide was characterized for its chemical, physical, thermal, and structural properties using HPLC, GPC, XRD, FTIR, TGA, UV-Vis spectroscopy, and NMR and the results of this characterization showed a characteristic feature of a typical polysaccharide...
October 17, 2018: International Journal of Biological Macromolecules
J Czerwinska, M C Parkin, P I Dargan, C George, A T Kicman, V Abbate
Mephedrone is a new psychoactive substance known to be unstable in biological matrices stored at room temperature or refrigerated. While the instability of mephedrone has been investigated before, there is currently no data regarding the stability of mephedrone metabolites. In this study, a liquid chromatography tandem mass spectrometry method for the simultaneous quantification of mephedrone and five of its phase I metabolites (dihydro-mephedrone, nor-mephedrone, hydroxytolyl-mephedrone, 4-carboxy-mephedrone and dihydro-nor-mephedrone) in human whole blood has been developed and validated...
October 20, 2018: Drug Testing and Analysis
Zhiyuan Meng, Xiaojun Chen, Lingjun Guan, Zhiying Xu, Qingxia Zhang, Yueyi Song, Fang Liu, Tianle Fan
Because of the increase of people's attention to food safety, monitoring the residue of pesticide in rice is becoming more and more important. Commercial and home processing techniques have been used to transform paddy rice into rice products for human or animal consumption, which may reduce the pesticide content in rice. The degradation of tricyclazole during different stages of commercial and home processing and storage was assessed in this paper. Many researches studying the occurrence and distribution of pesticide residues during rice cropping and processing have been reported...
October 19, 2018: Environmental Science and Pollution Research International
Fouad Fadhil Al-Qaim, Zainab Haider Mussa, Ali Yuzir, Jalifah Latip, Mohamed Rozali Othman
Prazosin (PRZ) and levonorgestrel (LNG) are widely used as an anti-disease drugs due to their biological activity in the human body. The frequent detection of these compounds in water samples requires alternative technologies for the removal of both compounds. After electrochemical degradation of PRZ and LNG, the parent compounds could be completely removed after treatment, but the identification and characterization of by-products are necessary as well. In this study, the effects of NaCl concentration and applied voltage were investigated during the electrochemical degradation process...
December 2018: Journal of Environmental Sciences (China)
Danni Yang, Yi Yang, Yongxin Li, Shuo Yin, Yaling Chen, Jiamin Wang, Jiangyu Xiao, Chengjun Sun
Background: Consistent toxicological evidence indicate that phthalates can cause adverse effects on human health. The concern over phthalate pollution and exposure has been emphasized in recent years. Therefore, the sensitive, reliable, and rapid detection of phthalates in water is of great importance. Objective: In this study, dispersive liquid-liquid microextraction based on solidification of floating organic droplet (DLLME-SFO) combined with HPLC-UV detection was established and applied in the preconcentration and detection of 15 phthalates in drinking and river water samples...
October 19, 2018: Journal of AOAC International
Mukhtar Ikhsan, Nurul Hiedayati, Kazutaka Maeyama, Fariz Nurwidya
OBJECTIVE: Nigella sativa (N. sativa) has several pharmacological actions which include antioxidant, antidiabetic, anticancer, antitussive, immunomodulator, analgesic, antimicrobial, anti-inflammatory, spasmolytic, and bronchodilator. The purpose of this study is to measure the effectivity of N. sativa ethanol extract as anti-inflammation on peritoneal Wistar rat mast cells. The laboratory experiment was used to investigate the effectivity of N. sativa as an anti-inflammatory on mast cells...
October 19, 2018: BMC Research Notes
Yen-Jung Chou, Jiunn-Jye Chuu, Yi-Jen Peng, Yu-Hsuan Cheng, Chin-Hsien Chang, Chieh-Min Chang, Hsia-Wei Liu
BACKGROUND: Osteoarthritis (OA) is a slow progressing, degenerative disorder of the synovial joints. Guilu Erxian Glue (GEG) is a multi-component Chinese herbal remedy with long-lasting favorable effects on several conditions, including articular pain and muscle strength in elderly men with knee osteoarthritis. The present study aimed to identify the effects of Guilu Erxian Paste (GE-P) and Liquid (GE-L) extracted from Guilu Erxian Glue in anterior cruciate ligament transection (ACLT)-induced osteoarthritis mice, and to compare the effectiveness of different preparations on knee cartilage degeneration during the progression of osteoarthritis...
October 19, 2018: Journal of Orthopaedic Surgery and Research
Bruna Maria Pereira da Costa Cordeiro, Nataly Diniz de Lima Santos, Magda Rhayanny Assunção Ferreira, Larissa Cardoso Corrêa de Araújo, Alexsander Rodrigues Carvalho Junior, Alan Diego da Conceição Santos, Ana Paula de Oliveira, Alexandre Gomes da Silva, Emerson Peter da Silva Falcão, Maria Tereza Dos Santos Correia, Jackson Roberto Guedes da Silva Almeida, Luís Cláudio Nascimento da Silva, Luiz Alberto Lira Soares, Thiago Henrique Napoleão, Márcia Vanusa da Silva, Patrícia Maria Guedes Paiva
BACKGROUND: Spondias tuberosa is a plant that produces a fruit crop with high economic relevance at Brazilian Caatinga. Its roots and leaves are used in folk medicine. METHODS: Chemical composition of a hexane extract from S. tuberosa leaves was evaluated by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC) and 1 H nuclear magnetic resonance (NMR). Antioxidant potential was investigated by DPPH and ABTS assays. Antifungal action on Candida species was evaluated determining the minimal inhibitory concentration (MIC50 ) and putative mechanisms were determined by flow cytometry analysis...
October 19, 2018: BMC Complementary and Alternative Medicine
Yashu Chen, Qin Mu, Kai Hu, Mo Chen, Jifang Yang, Jigang Chen, Bijun Xie, Zhida Sun
Menaquinone (MK) has an important role in human metabolism as an essential vitamin (VK₂), which is mainly produced through the fermentation of microorganisms. MK₈(H₂) was identified to be the main menaquinone from Rhodococcus sp. B7740, a bacterium isolated from the arctic ocean. In this work, MK₈(H₂) (purity: 99.75%) was collected through a convenient and economic extraction process followed by high-speed countercurrent chromatography (HSCCC) purification. Additionally, high-resolution mass spectrometry (HRMS) was performed for further identification and the hydrogenation position of MK₈(H₂) (terminal unit) was determined using nuclear magnetic resonance (NMR) for the first time...
October 18, 2018: Marine Drugs
Hubert Antolak, Agata Czyżowska, Dorota Kręgiel
Acetic acid bacteria belonging to the genus Asaia spp. are relatively new microbial contaminants in the beverage industry. These bacteria cause organoleptic changes such as increased turbidity, haziness and sour odor. In addition, they are able to form biofilms on the inner parts of production lines, and finally they can cause secondary contamination of final products. For this reason, new methods using effective and safe preservatives are being developed to improve microbial stability of soft beverages. The aim of the research was to investigate the effects of Mentha piperita L...
October 18, 2018: Foods (Basel, Switzerland)
Marianne Köck-Schulmeyer, Cristina Postigo, Marinella Farré, Damià Barceló, Miren López de Alda
Pollution has been less investigated in marine and coastal environments than in inland waters. The low levels at which pollutants are expected to be present in seawater calls for the use of reliable and high sensitivity analytical methodologies. In this context, this work presents the optimization and validation of an analytical method to determine 26 medium to highly polar pesticides in seawater based on solid phase extraction and liquid chromatography-tandem mass spectrometry detection. The developed methodology was linear, accurate (relative recoveries within 80-120% for most analytes), repeatable (relative standard deviations <18% for most analytes), and sensitive (limits of determination <1 ng/L for 89% of the compounds)...
October 8, 2018: Chemosphere
Maria Inês da Costa Marinho, Anna Isabel Guido Costa, Nívea Moreira Vieira, Maria Carolina Gomes Paiva, Francisco Cláudio Lopes de Freitas, Antonio Alberto da Silva
A modified QuEChERS (quick, easy, cheap, effective, rugged and safe) method was validated and applied for the estimation of half-lives of two imidazolinone group herbicides, imazethapyr and imazapic, in the Dystric Plinthosol (FX) and Ferralsol (LVA) agricultural soils using liquid chromatography tandem mass spectrometry. The solutions were prepared in a matrix extract to avoid matrix effects. The analytical method showed satisfactory specificity, selectivity, linearity (R2 > 0.99), recoveries (range 85...
October 16, 2018: Ecotoxicology and Environmental Safety
Baoxin Xu, Congcong Qu, Wenhua Zheng, Yaya Xi, Xin Zhao, Huifen Li, Jiangting Liu, Xuelan Zhang
Radix Polygala (Yuanzhi in Chinese) is well-known in traditional Chinese medicine (TCM) and has been used for treatment of depression, brain protection, and memory improvement for thousands of years. This plant medicine is rich in saponins, glycolipids, and organic acids. The purpose of the current study was to develop a rapid, accurate, and sensitive ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for the simultaneous determination of the following seven active components of Radix Polygala extracts in rat plasma: sibiricose A5 (A5); sibiricose A6 (A6); 3,6'-disinapoyl sucrose (DSS); tenuifoliside A (TFSA); tenuifoliside B (TFSB); tenuifoliside C (TFSC); and 3,4,5-trimethoxycinnamic acid (TMCA)...
October 2, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Skaidre Jankovskaja, Junichi Kamiie, Melinda Rezeli, Lena Gustavsson, Yutaka Sugihara, Tasso Miliotis, Tautgirdas Ruzgas, György Marko-Varga
BACKGROUND: Reproducible quantification of drug transporter protein expression in tissues is important for predicting transporter mediated drug disposition. Many mass-spectrometry based transporter protein quantification methods result in high variability of the estimated transporter quantities. Therefore, we aimed to evaluate and optimize mass spectrometry-based quantification method for drug transporter proteins in tissues. MATERIALS AND METHODS: Plasma membrane (PM) proteins from mouse tissues were isolated by applying three extraction protocols: commercial plasma membrane extraction kit, tissue homogenization by Potter-Elvehjem homogenizer in combination with sucrose-cushion ultracentrifugation, and PM enrichment with Tween 40...
October 7, 2018: Journal of Pharmaceutical and Biomedical Analysis
Xiunan Jiang, Yiting Shen, Haixing Wang, Caihong Wang, Xiaoxia Ye, Zheng Xiang
Kaurenoic acid (KA), a kaurane diterpene found in several medicinal plants, is an active ingredient with potential anti-inflammatory, anticonvulsant, antibacterial and antitumor activities. In this work, an ultra-performance liquid chromatography-tandem mass spectrometry method (UPLC-MS/MS) was firstly developed and validated to quantify kaurenoic acid in rat plasma. Rhein was chosen as the internal standard (IS) and the plasma was processed with one-step acetonitrile protein precipitation; the chromatographic separation was achieved on a HSS T3 (2...
October 9, 2018: Journal of Pharmaceutical and Biomedical Analysis
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