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Liquid Liquid extraction

Zhuangzhuang Li, Ran Wen, Yiqing Du, Shaozhe Zhao, Pan Zhao, Haiqiang Jiang, Rong Rong, Qingtao Lv
A simple, sensitive and selective high-performance liquid chromatography electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed and validated for simultaneous determination and pharmacokinetic study of 15 active compounds (Saikosaponin A, Baicalin, Wogonin, Glycyrrhizic acid, Glycyrrhetinic acid, Albiflorin, Paeoniflorin, Liquiritin, Isoliquiritin, Liquiritigenin, Isoliquiritigenin, Cinnamic acid, Gallic acid, Wogonoside and Oroxylin A) in rat plasma. After a feasible protein precipitation using methanol for sample preparation, chromatographic separation was carried out with a Halo® C18 column (2...
December 8, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Jianbin Ye, Shanshan Zheng, Zhan Zhang, Feng Yang, Ke Ma, Yinjie Feng, Jianqiang Zheng, Duobin Mao, Xuepeng Yang
In this study, bacterial cellulose (BC) was synthesized by Acetobacter xylinum ATCC 23767 using tobacco waste extract (TWE) as a carbon source. Nicotine was found to be an inhibitory factor for BC synthesis, but it can be removed at pH 9.0 by steam distillation. After removing nicotine, the BC production was 2.27 g/L in TWE prepared with solid-liquid (S-L) ratio at 1:10. To further enhance the BC production, two fermentation stages were performed over 16 days by re-adjusting the pH to 6.5 at 7 days, after the first fermentation stage was completed...
December 10, 2018: Bioresource Technology
Diego Ballesteros-Vivas, Gerardo Álvarez-Rivera, Andrea Del Pilar Sánchez-Camargo, Elena Ibáñez, Fabián Parada-Alfonso, Alejandro Cifuentes
In this work, a multi-analytical platform that allows obtaining and characterizing high-added value compounds from natural sources is presented, with a huge potential in traditional medicine, natural products characterization, functional foods, etc. Namely, the proposed multi-analytical platform is based on the combination of pressurized liquid extraction (PLE), liquid chromatography (LC) and gas chromatography quadrupole time-of-flight mass spectrometry GC-q-TOF-MS(/MS), in vitro assays and modelling tools for guiding extraction optimization...
November 24, 2018: Journal of Chromatography. A
Russell J Diefenbach, Jenny H Lee, Richard F Kefford, Helen Rizos
Analysis of liquid biopsies and the identification of non-invasive biomarkers for the diagnosis and prognosis of solid tumors has grown exponentially over the last few years. This has led to an increasing number of commercial kits optimised for the purification of circulating free (cf) DNA and RNA/miRNA from biofluids such as plasma, serum and urine. To optimise and standardise current practices we sought to evaluate the performance of spin column-based and magnetic bead-based commercial kits. The following commercial cfDNA purification kits were analysed in this study: QIAamp circulating nucleic acid kit (Qiagen, Germany); Plasma/serum cell-free circulating DNA Purification midi kit (Norgen Biotek, Canada); QIAamp minElute ccfDNA mini kit (Qiagen); Maxwell RSC ccfDNA plasma kit (Promega, USA); MagMAX cell-free DNA isolation kit (Applied Biosystems, USA); and NextPrep-Mag cfDNA isolation kit (Bioo Scientific, USA)...
December 2018: Cancer Genetics
Haiyao Sun, Chunying Li, Yujiao Ni, Liping Yao, Hongwei Jiang, Xueting Ren, Yujie Fu, Chunjian Zhao
In the present study, an efficient ultrasonic/microwave-assisted extraction (UMAE) procedure for the polysaccharides from the fruit of Camptotheca acuminata (CAFP) was investigated and optimized. Under the optimum conditions (ratio of liquid to raw material 30 mL/g, microwave irradiation time of 20 min, microwave irradiation power of 570 W and a fixed ultrasonic power of 50 W obtained by the response surface analysis with Box-Behnken design, satisfactory yields of CAFP (6.81 ± 0.04%) were achieved...
February 15, 2019: Carbohydrate Polymers
Mohamed Hajji, Marwa Hamdi, Sabrine Sellimi, Ghada Ksouda, Hocine Laouer, Suming Li, Moncef Nasri
A novel water-soluble polysaccharide named PLP1 was successfully isolated from root barks of Periploca laevigata by hot water extraction and further purified by DEAE-sepharose chromatography. PLP1 has a relative molar mass of 5.57 105 g/mol determined by Ultra-high performance liquid chromatography (UHPLC). The PLP1 structure was investigated by chemical and instrumental analysis including gas chromatography mass spectrometry (GC-MS), methylation analysis, Fourier transform infrared (FT-IR) spectroscopy and nuclear magnetic spectroscopy (1 H NMR and 13 C NMR)...
February 15, 2019: Carbohydrate Polymers
Miguel de Figueiredo, Christophe B Y Cordella, Delphine Jouan-Rimbaud Bouveresse, Xavier Archer, Jean-Marc Bégué, Douglas N Rutledge
Recent research efforts in the domain of fire debris analysis have been mainly oriented towards the development of innovative analytical procedures and chemometric approaches for the detection and classification of ignitable liquids in fire specimens according to the ASTM E1618. However, less attention has been brought to the question of the source inference of ignitable liquids. Infer the identity of source of ignitable liquids recovered from arson sites is still a challenging and ongoing research area. In this study, the objective is to link neat gasoline samples sharing a common source through the use of an untargeted chemometric approach applied to data acquired by automated thermodesorption (ATD)-GC-MS following passive headspace extraction onto Tenax TA tubes...
December 5, 2018: Forensic Science International
Hai-Fang Chen, Chen Zhang, Yun Yao, Jun-Mao Li, Wen-di Du, Mu-Lan Li, Bei Wu, Shi-Lin Yang, Yu-Lin Feng, Wu-Gang Zhang
TongFengTangSan (TFTS), a traditional Tibetan medicine comprising of Tinospora sinensis (TS), Terminalia chebula Retz (TC) and Trogopterori faeces (TF), is used to treat joint diseases like gout, gout arthritis, swelling, pain etc. Despite the significant therapeutic effects of TFTS, its pharmacological components have not been analyzed so far. Therefore, the chemical composition of the effective part of TFTS was analyzed by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS)...
November 17, 2018: Journal of Pharmaceutical and Biomedical Analysis
Libo He, Shiyan Zhang, Chaomei Luo, Yiran Sun, Qiuxia Lu, Lei Huang, Fang Chen, Lin Tang
Penthorum chinense Pursh (PCP), a medicinal and edible plant, is traditionally used for liver protection and treatment of liver diseases. In this study, we compared the differences of composition and activity of flowers, stems and leaves of PCP to select a bioactive part. The stems of PCP with stronger antioxidant activity (6.25-100 μg/mL) and lower cytotoxicity (25-200 μg/mL) than the flowers and leaves were a better bioactive part. Then the chemical composition and hepatoprotective effects of an aqueous extract and an 70% ethanolic extract made with stems of PCP were investigated...
December 14, 2018: Plant Foods for Human Nutrition
Xianyu Liang, Fan Liu, Yiqun Wan, Xiaoying Yin, Weiting Liu
In this study a method is developed for quantitative analysis of three potential biomarkers, including 4-hydroxyphenyl acetic acid (PHPAA), 4-hydroxyphenyl lactic acid (PHPLA) and 3,4-hydroxyphenylpropionic acid (PHPA), in human urine. Molecular imprinted polymers (MIPs) as the sample clean up materials were applied to selectively extract these tyrosine metabolites, followed by precise detection using ultra-high performance liquid chromatography coupled with a fluorescence detector (UHPLC-FLD). The MIP was prepared by precipitation polymerization adopting PHPAA as the template molecule, 1-vinylimidazole (1-vinyl) as functional monomer, trimethylolpropane triacrylate (TRIM) as crosslinker, 2-methylpropionitrile (AIBN) as initiator and acetonitrile as a porogen...
December 10, 2018: Journal of Chromatography. A
Edwin Heavisides, Caroline Rouger, Anna F Reichel, Cornelia Ulrich, Arlette Wenzel-Storjohann, Susanne Sebens, Deniz Tasdemir
The metabolism of seaweeds depends on environmental parameters, the availability of nutrients, and biotic/abiotic stresses; therefore, their chemical composition fluctuates throughout the year. This study investigated seasonal variations in the metabolome of the Baltic Sea brown alga Fucus vesiculosus and its potential relation to the bioactivity profile. By using a definitive screening design (DSD) combined with pressurised liquid extraction (PLE), an optimised protocol was developed to extract algal biomass monthly for a full calendar year...
December 13, 2018: Marine Drugs
Gui-Fang Feng, Shu Liu, Zi-Feng Pi, Feng-Rui Song, Zhi-Qiang Liu
In this study, a novel analysis strategy for progressively targeted screening and characterization of drug ingredients from in vitro to in vivo was proposed based on ultra-high performance liquid chromatography-tandem mass spectrometry for comprehensive characterization of in vivo metabolic profile of Polygalae radix (PR). First, an in vitro chemical profile of PR was described with the assistance of UNIFI™ software. The characteristic neutral small molecule losses were summarized to distinguish different chemical structures in the PR extract...
December 5, 2018: Journal of Pharmaceutical and Biomedical Analysis
Navroop Kaur Kalsi, Pervinder Kaur
The study was conducted to evaluate the influence of physicochemical properties of soil, moisture and temperature on the dissipation behaviour of bispyribac sodium under laboratory conditions. Bispyribac sodium residues were extracted using matrix solid phase dispersion and were quantified using liquid chromatography tandem-mass spectrometry. The mean percent recovery of bispyribac sodium from studied soils ranged from 82.7 ± 8.3-105.1± 2.6%. The limit of quantification and limit of detection was 0...
December 11, 2018: Ecotoxicology and Environmental Safety
Paula Renata Alves da Silva, Márcia Soares Vidal, Cleiton de Paula Soares, Valéria Polese, Michelle Zibetti Tadra-Sfeir, Emanuel Maltempi de Souza, Jean Luiz Simões-Araújo, José Ivo Baldani
The stalk apoplast fluid of sugarcane contains different sugars, organic acids and amino acids that may supply the demand for carbohydrates by endophytic bacteria including diazotrophs P. tropica (syn. B. tropica) strain Ppe8, isolated from sugarcane, is part of the bacterial consortium recommended as inoculant to sugarcane. However, little information has been accumulated regarding this plant-bacterium interaction considering that it colonizes internal sugarcane tissues. Here, we made use of the RNA-Seq transcriptomic analysis to study the influence of sugarcane stalk apoplast fluid on Ppe8 gene expression...
2018: PloS One
G H Yang, D M Han, H R Zang, T Wang, L F Li
Objective: The aim of this study is to investigate the effect of polyethylene glycol acrylate hybrid hydrogel degradation substance on nasal mucociliary transport system. Method: In our experiment, the complex hydrogel were formed by the combination of different ratios of polyethyleneglycol diacrylate, chitosan and polyvinyl alcohol. The experiment was divided into four groups according to the international standard of medical devices. DMEM/F12∶BEGM culture medium was used for each packing material extraction, DMEM/F12∶BEGM culture medium was used as control...
December 2018: Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery
Michael McCullagh, Kevin Giles, Keith Richardson, Sara Stead, Martin Palmer
Fluoroquinolones (FLQs) are a class of antimicrobial agents which have been administered to livestock for infection prevention/control and growth promotion. In this work blank porcine muscle extract (fortified with FLQs) has been screened for the FLQ protomer species (resulting from different sites of protonation) using the cumulative specificity of Ultra High Performance Liquid Chromatography coupled with Ion Mobility - Mass Spectrometry (UHPLC-IM-MS) and collision induced dissociation (CID). Collision cross sections (CCSs) and product ion spectra have been generated for the protomeric species with ΔCCS values between protomers ranging between 10-15 Å2 ...
December 14, 2018: Rapid Communications in Mass Spectrometry: RCM
Qiong Wang, Ping Fang, Lulu Zheng, Lei Ye
To characterize the preclinical plasma pharmacokinetics of entrectinib, a reproducible and precise assay is necessary. In this study, we developed and validated a simple ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the measurement of entrectinib using carbamazepine as the internal standard (IS) in rat plasma. Sample preparation was a simple protein precipitation with acetonitrile, then entrectinib was eluted on an Acquity UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) using a gradient elution with a mobile phase composed of acetonitrile (A) and 0...
December 13, 2018: Biomedical Chromatography: BMC
Mevlut Albayrak, Yucel Kadioglu, Mehmet Emrah Yaman, Onur Senol, Elif Oral
Olanzapine is an atypical antipsychotic drug from thienobenzodiazepine family which displays efficacy in patients with schizophrenia and related psychoses. A novel LC/MS method was developed and validated for determination of olanzapine in schizophrenia patients plasma. A liquid-liquid extraction procedure carried out by using 5 mL diethyl ether-diisopropyl ether mixture (1:1, v/v). Average recovery of the extraction procedure was found as 94.8 %. Chromatographic separation was performed on reversed-phase C18 column (250x2...
December 13, 2018: Biomedical Chromatography: BMC
Bo Zhong, Yaxin Li, Nethrie Idippily, Aaron Petty, Bin Su, Bingcheng Wang
Compound 27 [1, 12-bis(4-(4-amino-6,7-dimethoxyquinazolin-2-yl)piperazin-1-yl)dodecane-1,12-dione ] is a novel small molecule agonist of EphA2 receptor Tyrosine Kinase. It showed much improved activity for the activation of EphA2 receptor compared to the parental compound Doxazosin. To support further pharmacological and toxicological studies of the compound, a method using liquid chromatography and electrospray ionization tandem mass spectrometry (LC- MS/MS) has been developed for the quantification of this compound...
December 12, 2018: Biomedical Chromatography: BMC
Aiqian Li, Zhifeng Du, Mei Liao, Yulin Feng, Hanli Ruan, Hongliang Jiang
INTRODUCTION: Lycorine, one of the most common alkaloids in Lycoris spp., is believed to possess pharmacological activity. OBJECTIVE: To discover and identify lycorine-type alkaloids in the crude extracts of bulbs from six Lycoris spp. by ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) detection. METHODOLOGY: A qualitative analytical method with a data mining strategy was utilised. Based on the fragmentation patterns of standards investigated in positive tandem mass spectrometry (MS/MS) mode, the fragmentation rules of lycorine-type alkaloids were summarised...
December 11, 2018: Phytochemical Analysis: PCA
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