keyword
MENU ▼
Read by QxMD icon Read
search

Sample preparation

keyword
https://www.readbyqxmd.com/read/30315809/holographic-characterization-of-protein-aggregates-in-the-presence-of-silicone-oil-and-surfactants
#1
Priya N O Kasimbeg, Fook Chiong Cheong, David B Ruffner, Jaroslaw M Blusewicz, Laura A Philips
Characterizing protein aggregates in the presence of silicone oil is a long standing challenge for the pharmaceutical industry. Silicone oil is often used as a lubricant in devices that deliver and store therapeutic protein products, and has been linked to protein aggregation, which can compromise a drug's effectiveness or cause autoimmune responses in patients. Most traditional technologies cannot quantitatively distinguish protein aggregates and silicone oil in their native formulations for sizes less than 5 μm...
October 10, 2018: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/30315760/rapid-detection-of-escherichia-coli-o157-h7-by-a-fluorescent-microsphere-based-immunochromatographic-assay-and-immunomagnetic-separation
#2
Qianru Li, Yuexi Yang, Fei Hu, Yanxue Cai, Xiaoyun Liu, Xiaowei He
To ensure food safety and avoid infections by Escherichia coli O157:H7 (E. coli O157:H7), we developed a novel fluorescent microsphere (FM)-based immunochromatography assay (FM-ICA). FMs were conjugated to anti-E. coli O157:H7 monoclonal antibody (MAb) as an ICA probe, Immunomagnetic beads (IM-beads) were prepared by conjugating functionalized magnetic microspheres with the antibody for enrichment and separation of pathogenic bacteria from complex food matrices. Under selected conditions, a standard curve of FM-ICA measurement of E...
October 10, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/30315698/infectious-causes-of-bovine-abortion-in-qazvin-province-iran
#3
A Kaveh, E Merat, S Samani, S Danandeh, S Soltan Nezhad
Abortion in dairy cattle is generally defined as the loss of fetus on days 47-265 of pregnancy and can significantly reduce the income and capital gain of farmers and producers. Therefore, sufficient economical support and preventive measurements are essential for this issue. In this study, genetic materials were extracted from the abomasum rennet, as well as homogenous preparations of brain, kidney, spleen, liver, and lung tissues of 128 aborted fetuses. Afterward, polymerase chain reaction (PCR) and Reverse Transcription Polymerase Chain Reaction (RT-PCR) tests were performed to identify the bovine viral diarrhea virus (BVDV), bovine herpesvirus 1 (BHV-1), Neospora caninum parasite, and serovars of Leptospira species...
December 2017: Archives of Razi Institute
https://www.readbyqxmd.com/read/30315654/the-influence-of-light-sources-on-sunitinib-measurements-with-photoisomerization
#4
Noriko Matsunaga, Takashi Kitahara, Makiko Yamada, Kayoko Sato, Yukinobu Kodama, Hitoshi Sasaki
Sunitinib is an orally administered tyrosine kinase inhibitor. Therapeutic drug monitoring is an important component of the follow-up of patients because of high interpatient variability in the pharmacokinetics of sunitinib and large variabilities in its efficacy and toxicity. The aim of the present study was to examine the light stability of sunitinib and confirm the effects of light exposure on sunitinib measurements by LC-MS/MS. Sunitinib and its active metabolite, SU12662 convert Z isomers to E isomers with exposure to light...
October 13, 2018: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/30315647/cetyl-alcohol-reinforced-hollow-fiber-solid-liquid-phase-microextraction-and-hplc-dad-analysis-of-ezetimibe-and-simvastatin-in-human-plasma-and-urine
#5
Nabil N Al-Hashimi, Rand O Shahin, Aqeel N Al-Hashimi, Ajeal M Al Ajeal, Lubna H Tahtamouni, Chanbasha Basheer
A new cetyl-alcohol-reinforced hollow fiber solid/liquid phase microextraction (CA-HF-SLPME) followed by HPLC-DAD method was developed for simultaneous determination of ezetimibe and simvastatin in human plasma and urine samples. To prepare the CA-HF-SLPME device, the cetyl-alcohol was immobilized into the pores of 2.5 cm hollow fiber micro-tube and the lumen of the micro-tube was filled with 1-octanol with the two ends sealed. Afterward, the prepared device was introduced into 10 mL of the sample solution containing the analytes with agitation...
October 13, 2018: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/30315508/diagnostic-value-of-energy-dispersive-hand-held-x-ray-fluorescence-spectrometry-in-determining-trace-element-concentrations-in-ovine-liver
#6
Daniël E van Loggerenberg, Pete N Laver, Jan G Myburgh, Christo J Botha
There are no data available on the use of hand-held X-ray fluorescence (XRF) spectrometry to determine trace element concentrations in veterinary diagnostics. The hand-held XRF spectrometer is easy to use and does not require extensive training for the operator. In Sub-Saharan Africa with few centralised analytical laboratories equipped with expensive apparatus or mass spectrometry capabilities, trace element analysis using the hand-held XRF spectrometer provides an alternative. The objective of this study was to compare ovine hepatic copper (Cu), iron (Fe), manganese (Mn), molybdenum (Mo), selenium (Se) and zinc (Zn) concentrations as obtained with the hand-held XRF spectrometer to those of a reference laboratory using inductively coupled plasma mass spectrometry (ICP-MS)...
October 13, 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/30314335/highly-sensitive-humidity-sensor-based-on-oblique-carbon-nanoplumes
#7
Siqi Qiao, Xiaoyan Peng, Lidan Wang, Shukai Duan, Jin Chu, Pengfei Jia
In this work, we fabricated three carbon nanoplume structured samples under different temperatures using a simple hot filament physical vapor deposition (HFPVD) process, and investigated the role of surface morphology, defects, and graphitic content on relative humidity (RH) sensing performances. The Van der Drift growth model and oblique angle deposition (OAD) technique of growing a large area of uniformly aligned and inclined oblique arrays of carbon nanoplumes (CNPs) on a catalyst-free silicon substrate was demonstrated...
October 11, 2018: Sensors
https://www.readbyqxmd.com/read/30314319/potential-use-of-plastic-wastes-for-low-thermal-conductivity-concrete
#8
Artid Poonyakan, Manaskorn Rachakornkij, Methi Wecharatana, Watanachai Smittakorn
The use of plastics has increased over the years, thus resulting in a large volume of plastic waste being generated and accumulated in the environment. Due to its non-biodegradability and persistence, recycling processes have become one of the sustainable solutions for preventing environmental deterioration. Plastic wastes, including high density polyethylene (HDPE), low density polyethylene (LDPE), polypropylene (PP), and polyethylene terephthalate (PET), were collected from industrial sector and used as additional ingredients to improve concrete properties...
October 11, 2018: Materials
https://www.readbyqxmd.com/read/30312950/effect-of-phase-composition-on-the-photocatalytic-activity-of-titanium-dioxide-obtained-from-supercritical-antisolvent
#9
Elisangela P Da Silva, Manuel E G Winkler, Willyan M Giufrida, Lucio Cardozo-Filho, Christian G Alonso, Jardel B O Lopes, Adley F Rubira, Rafael Silva
Photocatalytic activity of TiO2 nanoparticles is highly dependent on their phase composition. The coexistence of anatase and rutile phases in a single nanoparticle eases the electron transfer process between the phases, and favors the separation of photogenerated pairs. In this work, highly photoactive mixed-phase TiO2 nanostructures were prepared by supercritical antisolvent precipitation (SAS), an environmentally friendly technology. It is shown here that this methodology has the remarkable ability to produce highly porous (515 m2 /g) and crystalline TiO2 nanoparticles...
September 29, 2018: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/30312748/development-and-functional-characterization-of-composite-freeze-dried-wafers-for-potential-delivery-of-low-dose-aspirin-for-elderly-people-with-dysphagia
#10
Smirna Farias, Joshua S Boateng
The impact of demographic ageing is likely to be of major significance in the coming decades due to low birth rates and higher life expectancy. Older people generally require more prescribed medicines due to the presence of multiple conditions such as dysphagia which can make swallowing medicines challenging. This study involves the development, characterization and optimization of composite wafers for potential oral and buccal delivery of low dose aspirin to prevent thrombosis in elderly patients with dysphagia...
October 9, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/30312719/a-hybrid-of-mpeg-b-pcl-and-g1-pea-dendrimer-for-enhancing-delivery-of-antibiotics
#11
Calvin A Omolo, Rahul S Kalhapure, Nikhil Agrawal, Mahantesh Jadhav, Sanjeev Rambharose, Chunderika Mocktar, Thirumala Govender
The development of novel materials is essential for the efficient delivery of drugs. Therefore, the aim of the study was to synthesize a linear polymer dendrimer hybrid star polymer (3-mPEA) comprising of a generation one poly (ester-amine) dendrimer (G1-PEA) and a diblock copolymer of methoxy poly (ethylene glycol)-b-poly(ε-caprolactone) (mPEG-b-PCL) for formulation of nanovesicles for efficient drug delivery. The synthesized star polymer was characterized by FTIR, 1 H and 13 C NMR, HRMS, GPC and its biosafety was confirmed by MTT assays...
October 9, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/30312412/investigation-of-xylanase-diet-formulation-method-for-energy-and-choice-of-digestibility-index-marker-on-nutrient-and-energy-utilization-for-broiler-chickens-and-pigs
#12
T Wang, M R Bedford, O Adeola
The aim of this study was to investigate the growth performance and nutrient utilization responses of broiler chickens and the nutrient utilization of pigs to xylanase, experimental diet formulation method for energy (FME), and digestibility index marker (DIM). In Exp. 1, a total of 448 male broiler chickens were used in a randomized complete block design with body weight as a blocking factor. Seven dietary treatments were prepared in a 3 × 2 + 1 factorial arrangement with inclusion of sand, diatomaceous earth (DAE), or wheat bran (WB) as FME and without or with xylanase (26,400 unit/kg of diet) plus positive control...
October 12, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/30312029/tannic-acid-solution-a-better-fixative-solution-than-formalin-for-elastin-and-collagen-toxic-and-morphological-assessment
#13
Rafael Cisne, Alessandra M T Souza, Marco A Pereira-Sampaio, Marcio A Babinski, Silvana L Gorniak, Paula C Papa
Formaldehyde is commonly used worldwide, even though it is classified as carcinogenic to humans by the International Agency for Research on Cancer. This has motivated intensive investigations of formaldehyde substitutes, and recently, some alternative solutions were found, which can potentially replace it. Previous research showed that tannic acid (TA) in glutaraldehyde solution has the ability to stabilize elastin and collagen. This provided a basis for the development of a new alcoholic fixative solution, particularly aimed at extracellular matrix components, with TA as a main component...
September 2018: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/30311954/accuracy-of-hydrocortisone-dose-administration-via-nasogastric-tube
#14
E Daniel, M J Whitaker, B Keevil, J Wales, R J Ross
OBJECTIVE: Hydrocortisone via nasogastric (NG) tube is used in sick children with adrenal insufficiency; however, there is no licensed formulation for NG administration. METHODS: We investigated hydrocortisone recovery after passage through NG tubes in vitro for 3 formulations: liquid suspension, crushed tablets mixed with water, and hydrocortisone granules designed for oral administration to children. Cortisol was measured by LC-MS/MS. RESULTS: Hydrocortisone content was variable and recovery low after preparation in syringe and prior to passage through NG tubes...
October 12, 2018: Clinical Endocrinology
https://www.readbyqxmd.com/read/30311901/stability-studies-and-characterization-of-glutathione-loaded-nanoemulsion
#15
Naveed Ullah Khan, Atif Ali, Hira Khan, Zaheer Ullah Khan, Zia Ahmed
Glutathione reduced (GSH) is the mother of all the antioxidants and has an antimelanogenic effect. It is extremely vulnerable to oxidation in the solution form which limits its use. The GSH in nano-oil droplets present a potential solution to this problem. The aim of this study was to formulate glutathione-loaded nanoemulsion and assess its stability studies over a 90-day testing period. To formulate GSH-loaded nanoemulsion pseudo-ternary phase diagram, it was built with various concentrations of water, liquid paraffin oil, and surfactant mixture (Tween 80 and Span 80)...
July 2018: Journal of Cosmetic Science
https://www.readbyqxmd.com/read/30311686/recommendations-for-conducting-invasive-urodynamics-for-men-with-lower-urinary-tract-symptoms-qualitative-interview-findings-from-a-large-randomized-controlled-trial-upstream
#16
Lucy E Selman, Cynthia A Ochieng, Amanda L Lewis, Marcus J Drake, Jeremy Horwood
AIMS: To capture in-depth qualitative evidence regarding attitudes to and experiences of urodynamic testing among men with lower urinary tract symptoms (LUTS) at each end of the clinical pathway. METHODS: Semi-structured interview study conducted within the Urodynamics for Prostate Surgery: Randomized Evaluation of Assessment Methods (UPSTREAM) trial, which randomized men to a care pathway including urodynamics or routine non-invasive tests from 26 secondary care urology sites across England...
October 12, 2018: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/30311521/perspectives-in-professional-education-reassessing-courses-required-for-admission-to-colleges-of-veterinary-medicine-in-north-america-and-the-caribbean-to-decrease-stress-among-first-year-students
#17
James N Moore, Noah D Cohen, Scott A Brown
OBJECTIVE To identify courses in which first-year veterinary students struggled academically and to survey veterinarians as to their opinions on existing prerequisite courses and proposed alternatives. DESIGN Electronic surveys. SAMPLE Associate deans for academic affairs at colleges of veterinary medicine and practicing veterinarians in North America and the Caribbean. PROCEDURES Surveys were sent to associate deans of academic affairs seeking information on courses in which first-year veterinary students most commonly struggled academically...
November 1, 2018: Journal of the American Veterinary Medical Association
https://www.readbyqxmd.com/read/30310948/preparation-and-characterization-of-herbicide-mecoprop-imprinted-polymer-and-its-application-as-a-selective-sorbent-in-water-sample
#18
Elaheh Mansouri, Mahboobe Sarabi-Jamab, Behrouz Ghorani, Seyed Ahmad Mohajeri
Bulk polymerization method was used to prepare a homogeneous molecularly imprinted polymer (MIP) for the specific extraction of herbicide mecoprop (MCPP). Thereafter, the binding performance of this functional polymer was evaluated under optimal condition, compared to a non-imprinted polymer. From the Scatchard plot analysis, two types of binding sites were detected in the MIP, the high affinity binding sites with a KD (equilibrium dissociation constant) of 6.4 µM and the low affinity ones with a KD of 55...
October 11, 2018: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/30310395/effects-of-carbon-dioxide-insufflation-on-anastomosis-remodeling-at-a-carotid-artery-site-in-rabbits
#19
Tuğra Gençpınar, Gökmen Akkaya, Çağatay Bilen, Pınar Akokay, Osman Yılmaz, Hudai Çatalyürek
Introduction: Use of carbon dioxide (CO2 ) insufflation (CDI) on the surgical field during heart surgery has become widespread, and in some units routine. Aim: To assess the effects of CDI on endothelial dysfunction in a carotid artery model in rabbits. Material and methods: Twelve randomly selected rabbits were divided into two groups. Right carotid arteries of the animals were transected and sutured with running suture technique. Then, 1 l/min CO2 insufflation was initiated with a 45° angle...
September 2018: Kardiochirurgia i Torakochirurgia Polska, Polish Journal of Cardio-Thoracic Surgery
https://www.readbyqxmd.com/read/30310353/two-new-species-of-genus-limnias-from-thailand-with-keys-to-congeners-rotifera-gnesiotrocha
#20
Phuripong Meksuwan, Rapeepan Jaturapruek, Supiyanit Maiphae
Two new species and two morphological variant taxa of sessile rotifers found during a survey of Thai rotifers are reported upon. Living specimens were collected and identified from twelve sampling sites. The specimens were photographed, and prepared for SEMs of dorsal plates and trophi. Two new species of genus Limnias are recognized. Limniaslenis sp. n. possesses a corona with a relatively shallow ventral sinus, and a dorsal plate without any projection, whereas Limniasnovemceras sp. n. carries a corona with a deep and wide ventral sinus, and a dorsal plate with nine projections...
2018: ZooKeys
keyword
keyword
166758
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"