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anaerobic lignin degradation

Casey A Hooker, Ethan T Hillman, Jonathan C Overton, Adrian Ortiz-Velez, Makayla Schacht, Abigail Hunnicutt, Nathan S Mosier, Kevin V Solomon
Background: Plant biomass is an abundant but underused feedstock for bioenergy production due to its complex and variable composition, which resists breakdown into fermentable sugars. These feedstocks, however, are routinely degraded by many uncommercialized microbes such as anaerobic gut fungi. These gut fungi express a broad range of carbohydrate active enzymes and are native to the digestive tracts of ruminants and hindgut fermenters. In this study, we examine gut fungal performance on these substrates as a function of composition, and the ability of this isolate to degrade inhibitory high syringyl lignin-containing forestry residues...
2018: Biotechnology for Biofuels
Snehasish Mishra, Puneet Kumar Singh, Swagatika Dash, Ritesh Pattnaik
Biogas obtained from organic remains entails a developed technology and an appreciable methane yield, but its use may not be sustainable. The potential methane yield of various lignocellulose biomass and the operational conditions employed are inherently reviewed. Although of lower methane yields compared to conventional substrates, agricultural biomass is a cheap option. The major challenges encountered during its biogasification are its recalcitrance nature primarily due to the presence of crystalline cellulose and lignin...
November 2018: 3 Biotech
E Hosseini Koupaie, S Dahadha, A A Bazyar Lakeh, A Azizi, E Elbeshbishy
The rapid depletion of natural resources and the environmental concerns associated with the use of fossil fuels as the main source of global energy is leading to an increased interest in alternative and renewable energy sources. Particular interest has been given to the lignocellulosic biomass as the most abundant source of organic matter with a potential of being utilized for energy recovery. Different approaches have been applied to convert the lignocellulosic biomass to energy products including anaerobic digestion (AD), fermentation, combustion, pyrolysis, and gasification...
October 9, 2018: Journal of Environmental Management
Wu Li, Qing Li, YuanYuan Wang, Longyu Zheng, Yanlin Zhang, Ziniu Yu, Huanchun Chen, Jibin Zhang
Corncob degradation is an issue that needs to be address for it to be further utilized as bioenergy. We explored a new comprehensive degradation strategy for corncob. First, restaurant wastewater was used to improve the corncob biochemical characteristics and partly degrade the lignocelluloses. After the restaurant wastewater treatment, the residue was converted using black soldier fly larvae (BSFL), and the supernatant was utilized for biogas production by anaerobic fermentation. The highest product rates of glucose, xylose, and arabinose were obtained at the optimal corncob soaking condition at 75 °C, 5 h, and 60 g/L from lignocellulose...
December 1, 2018: Journal of Environmental Management
Huayong Zhang, Xiaoxi Han, Yonglan Tian, Ying Li, Kun Yang, He Hao, Yang Chai, Xiang Xu
Anaerobic fermentation is considered as a cost-effective way of biomass waste disposal. Chromium (Cr) is one of the heavy metals that often been blamed for unsatisfactory operation or failure of anaerobic fermentation. The impact of Cr (added as K2 Cr2 O7 ) on mesophilic anaerobic fermentation of Phragmites australis straw and cow dung was demonstrated by investigating the biogas properties, process stability, substrate degradation and enzyme activities during the fermentation process. The results showed that 30, 100 and 500 mg/L Cr6+ addition increased the cumulative biogas yields by up to 19...
October 15, 2018: Journal of Environmental Management
Yun-Long Zhou, Zhi-Yang Xu, Ming-Xing Zhao, Wan-Sheng Shi, Zhen-Xing Huang, Di He, Wen-Quan Ruan
The model of high solid anaerobic digestion was used by improving the degree of homogeneity of the reaction system and biogas slurry reflux to gradually increase the material load. The vinegar residue-efficient anaerobic digestion system was successfully constructed without pretreatment.The optimum anaerobic digestibility was observed when the material loading of the reaction system reached 6.15 g·(L·d)-1 , when the amount of biogas produced per unit of dry material was 396 mL·g-1 , and the amount of methane produced per unit of dry material was 211 mL·g-1 ...
October 8, 2017: Huan Jing Ke Xue= Huanjing Kexue
Ayyappa Kumar Sista Kameshwar, Wensheng Qin
Ruminating animals, especially cattle lack the carbohydrate active enzyme encoding genes which are required for the degradation of the glycosidic linkages of plant cell wall carbohydrates (such as cellulose, hemicellulose, lignin and pectin). Thus, ruminating animals are completely dependent on the microorganisms (anaerobic bacteria and fungi, methanogenic archaea and protozoa) residing in their rumen (hindgut). In this study, we have retrieved and analyzed the complete genome wide annotations of the Neocallimastigomycota division fungi such as Anaeromyces robustus, Neocallismatix californiae, Orpinomyces sp, Piromyces finnis, Piromyces sp E2...
2018: Journal of Genomics
Ruolin Guan, Xiujin Li, Akiber Chufo Wachemo, Hairong Yuan, Yanping Liu, Dexun Zou, Xiaoyu Zuo, Junyu Gu
In order to determine eco-friendly pretreatment method, the combination of different pretreatment reagents such as: CaO, ammonia solution (AS), liquid fraction of digestate (LFD), CaO-AS and CaO-LFD were used in this study. The features of physico-chemical structures and anaerobic digestion (AD) performance of rice straw were investigated using different combined biological and chemical pretreatment methods. The results showed that CaO-LFD bio-chemical pretreatment achieved the best effect among different pretreatment conditions...
October 1, 2018: Science of the Total Environment
Daniel Girma Mulat, Janka Dibdiakova, Svein Jarle Horn
Background: The emerging cellulosic bioethanol industry will generate huge amounts of lignin-rich residues that may be converted into biogas by anaerobic digestion (AD) to increase the output of energy carriers from the biorefinery plants. The carbohydrates fraction of lignocellulosic biomass is degradable, whereas the lignin fraction is generally considered difficult to degrade during AD. The objective of this study was to investigate the feasibility of biogas production by AD from hydrolysis lignin (HL), prepared by steam explosion (SE) and enzymatic saccharification of birch...
2018: Biotechnology for Biofuels
Ahmed M Mustafa, Hui Li, Amira A Radwan, Kuichuan Sheng, Xiang Chen
The effect of hydrothermal (HTP) and Ca(OH)2 pretreatments on the biogas produced by the anaerobic digestion of sugarcane bagasse (SCB) was studied. HTP, Ca(OH)2 and combination pretreatment had significant effects on hemicellulose and lignin degradation during pretreatment and methane yield through digestion. The highest biogas production observed in combination pretreatment HTP 180 + 8.5% of SCB reached 318 mL/g Volatile Solids (VS), which were 47% higher than the untreated SCB, with the highest methane content 69% and highest lignin degradation 44%...
July 2018: Bioresource Technology
Jin-Kyu Park, Yong-Gil Chong, Kazuo Tameda, Nam-Hoon Lee
In the first order decay (FOD) model of landfill methane generation, the methane generation potential ( L0 ) and methane generation rate constant ( k) for both bulk municipal solid waste (MSW) and individual waste components have been determined by a variety of approaches throughout various literature. Differences in the determination methods for L0 and k are related to differences in our understanding of the waste decomposition dynamics. A thorough understanding of the various available methods for determining L0 and k values is critical for comparative study and the drawing of valid conclusions...
March 2018: Waste Management & Research
Jean H El Achkar, Thomas Lendormi, Dominique Salameh, Nicolas Louka, Richard G Maroun, Jean-Louis Lanoisellé, Zeina Hobaika
To optimize the anaerobic digestion of grape pomace under mesophilic conditions, continuous digesters were operated at different hydraulic retention times (HRT) (30, 20, 15 and 10 days) equivalent to organic loading rates (OLR) of 2.5, 3.7, 5.7 and 7.3 kg COD m-3 d-1 , respectively. At HRTs of 30 and 20 days, steady state conditions were observed with methane yields of 0.984 ± 0.013 NL d-1 and 1.362 ± 0.018 NL d-1 , respectively. The HRT of 15 days was found critical because of acids accumulation through the experiments...
January 2018: Waste Management
Roberto Orellana, Gina Chaput, Lye Meng Markillie, Hugh Mitchell, Matt Gaffrey, Galya Orr, Kristen M DeAngelis
The production of lignocellulosic-derived biofuels is a highly promising source of alternative energy, but it has been constrained by the lack of a microbial platform capable to efficiently degrade this recalcitrant material and cope with by-products that can be toxic to cells. Species that naturally grow in environments where carbon is mainly available as lignin are promising for finding new ways of removing the lignin that protects cellulose for improved conversion of lignin to fuel precursors. Enterobacter lignolyticus SCF1 is a facultative anaerobic Gammaproteobacteria isolated from tropical rain forest soil collected in El Yunque forest, Puerto Rico under anoxic growth conditions with lignin as sole carbon source...
2017: PloS One
Susanna Seppälä, St Elmo Wilken, Doriv Knop, Kevin V Solomon, Michelle A O'Malley
A wealth of fungal enzymes has been identified from nature, which continue to drive strain engineering and bioprocessing for a range of industries. However, while a number of clades have been investigated, the vast majority of the fungal kingdom remains unexplored for industrial applications. Here, we discuss selected classes of fungal enzymes that are currently in biotechnological use, and explore more basal, non-conventional fungi and their underexploited biomass-degrading mechanisms as promising agents in the transition towards a bio-based society...
November 2017: Metabolic Engineering
Shilva Shrestha, Xavier Fonoll, Samir Kumar Khanal, Lutgarde Raskin
Lignocellulosic biomass is the most abundant renewable bioresource on earth. In lignocellulosic biomass, the cellulose and hemicellulose are bound with lignin and other molecules to form a complex structure not easily accessible to microbial degradation. Anaerobic digestion (AD) of lignocellulosic biomass with a focus on improving hydrolysis, the rate limiting step in AD of lignocellulosic feedstocks, has received considerable attention. This review highlights challenges with AD of lignocellulosic biomass, factors contributing to its recalcitrance, and natural microbial ecosystems, such as the gastrointestinal tracts of herbivorous animals, capable of performing hydrolysis efficiently...
December 2017: Bioresource Technology
Javier Lizasoain, Adrian Trulea, Johannes Gittinger, Iris Kral, Gerhard Piringer, Andreas Schedl, Paal J Nilsen, Antje Potthast, Andreas Gronauer, Alexander Bauer
This study evaluated the effect of steam explosion on the chemical composition and biomethane potential of corn stover using temperatures ranging between 140 and 220°C and pretreatment times ranging between 2 and 15min. Biodegradation kinetics during the anaerobic digestion of untreated and corn stover, pretreated at two different intensities, 140°C for 5min and 180°C for 5min, were studied in tandem. Results showed that pretreatment at 160°C for 2min improved the methane yield by 22%. Harsher pretreatment conditions led to lower hemicellulose contents and methane yields, as well as higher lignin contents, which may be due to the formation of pseudo-lignin...
November 2017: Bioresource Technology
Michel Schroyen, Stijn W H Van Hulle, Sander Holemans, Han Vervaeren, Katleen Raes
The impact of various phenolic compounds, vanillic acid, ferulic acid, p-coumaric acid and 4-hydroxybenzoic acid on anaerobic digestion of lignocellulosic biomass (hemp straw and miscanthus) was studied. Such phenolic compounds have been known to inhibit biogas production during anaerobic digestion. The different phenolic compounds were added in various concentrations: 0, 100, 500, 1000 and 2000mg/L. A difference in inhibition of biomethane production between the phenolic compounds was noted. Hydrolysis rate, during anaerobic digestion of miscanthus was inhibited up to 50% by vanillic acid, while vanillic acid had no influence on the initial rate of biogas production during the anaerobic digestion of hemp straw...
November 2017: Bioresource Technology
Clament Fui Seung Chin, Yoshihide Furuya, Mohd Huzairi Mohd Zainudin, Norhayati Ramli, Mohd Ali Hassan, Yukihiro Tashiro, Kenji Sakai
Previously, a unique co-compost produced by composting empty fruit bunch with anaerobic sludge from palm oil mill effluent, which contributed to establishing a zero-emission industry in Malaysia. Little was known about the bacterial functions during the composting process and fertilization capacity of this co-compost. We isolated 100 strains from the co-compost on 7 types of enumeration media and screened 25 strains using in vitro tests for 12 traits, grouping them according to three functions: plant growth promoting (fixation of nitrogen; solubilization of phosphorus, potassium, and silicate; production of 3-indoleacetic acid, ammonia, and siderophore), biocontrolling (production of chitinase and anti-Ganoderma activity), and composting (degradation of lignin, xylan, and cellulose)...
November 2017: Journal of Bioscience and Bioengineering
Sameh S Ali, Abd El-Fatah Abomohra, Jianzhong Sun
Anaerobic digestion (AD) is considered an efficient cost-effective technology for sustainable biogas production from lignocellulosic wastes. A novel lignocellulosic degradation microbial consortium (LCDC) was isolated from rotten sawdust, and further used for sawdust pretreatment prior to AD. Results showed that pretreatment of sawdust for 10days led to significant reduction in cellulose, hemicelluloses, and lignin contents by 37.5%, 39.6%, and 56.7%, respectively, with respect to the control. In addition, the pretreatment enhanced cumulative biogas yield, which reached its maximum value of 312...
August 2017: Bioresource Technology
Robert J Gruninger, Adrian Tsang, Tim A McAllister
Fungi utilize a unique mechanism of nutrient acquisition involving extracellular digestion. To understand the biology of these microbes, it is important to identify and characterize the function of proteins that are secreted and involved in this process. Mass spectrometry-based proteomics is a powerful tool to study complex mixtures of proteins and understand how the proteins produced by an organism change in response to different conditions. Many fungi are efficient decomposers of plant cell wall, and anaerobic fungi are well recognized for their ability to digest lignocellulose...
2017: Methods in Molecular Biology
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