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Inflammation rats

Hao Yang, Bo Liu, Yajun Liu, Da He, Yonggang Xing, Yan An, Wei Tian
STUDY DESIGN: Rat nucleus pulposus (NP) cells or annulus fibrosus (AF) cells were stimulated with conditioned media of RAW 264.7 macrophages and vice versa under healthy culture conditions and in the presence of pro-inflammatory mediators. The gene expression of pro-inflammatory mediators, ECM-modifying enzymes, and chemokines, which play important roles in intervertebral disc degeneration (IDD), was determined. OBJECTIVE: To test whether the interaction between native disc cells and infiltrating macrophages accelerates inflammation state, disrupts matrix homeostasis, and promotes inflammatory cells infiltration...
December 11, 2018: Spine
Rui Zheng, Xiaoliang Fang, Lei He, Yanjiao Shao, Nana Guo, Liren Wang, Mingyao Liu, Dali Li, Hongquan Geng
BACKGROUND: Primary hyperoxaluria type 1 (PH1) is an inherited disease caused by mutations in alanine-glyoxylate aminotransferase (AGXT). It is characterized by abnormal metabolism of glyoxylic acid in liver leading to endogenous oxalate overproduction and deposition of oxalate in multiple organs, mainly the kidney. Patients of PH1 often suffer from recurrent urinary tract stones, and finally renal failure. There is no effective treatment other than combined liver-kidney transplantation...
December 11, 2018: Current Molecular Medicine
Roberto Mota, Matthew J Campen, Matthew E Cuellar, William S Garver, Jacob Hesterman, Mohammed Qutaish, Tamara Daniels, Monique Nysus, Carston R Wagner, Jeffrey P Norenberg
Atherosclerosis-related morbidity and mortality remain a global concern. Atherosclerotic disease follows a slow and silent progression, and the transition from early-stage lesions to vulnerable plaques remains difficult to diagnose. Inflammation is a key component of the development of atherosclerotic plaque and consequent life-threatening complications. This study assessed 111 In-DANBIRT as an in vivo , noninvasive SPECT/CT imaging probe targeting an inflammatory marker, Lymphocyte Function Associated Antigen-1 (LFA-1), in atherosclerotic plaques...
2018: Contrast Media & Molecular Imaging
Lin Feng, Xiaozhe Yang, Yanfeng Shi, Shuang Liang, Tong Zhao, Junchao Duan, Zhiwei Sun
Background: The harmful effects following the release of nanomaterials into environment are of great concern today. Purpose: In this study, subacute effect due to co-exposure to low-dose silica nanoparticles (SiNPs) and lead acetate (Pb) on cardiovascular system was detected in Sprague Dawley male rats. Materials and Methods: Histopathological and ultrastructural changes of heart, aortic arch and abdominal aorta were detected. Blood routine and blood biochemistry examinations were used to show the changes of blood components...
2018: International Journal of Nanomedicine
Zhen Zhang, Zheyi Li, Chengyao Zhang, Jiannan Liu, Yuxing Bai, Song Li, Chenping Zhang
Purpose: The purpose of this study was to assess the effects of biomimetic intrafibrillar mineralized collagen (IMC) bone scaffold materials on bone regeneration and the underlying biological mechanisms. Materials and methods: A critical-sized bone defect in the rat femur was created; then IMC, extrafibrillar mineralized collagen, and nano-hydroxyapatite bone scaffold materials were grafted into the defect. Ten weeks after implantation, micro-computed tomography and histology were applied to evaluate the bone regeneration...
2018: International Journal of Nanomedicine
Carleene Bryan, Ibrahim Sammour, Kasonya Guerra, Mayank Sharma, Fredrick Dapaah-Siakwan, Jian Huang, Ronald Zambrano, Merline Benny, Shu Wu, Karen Young
BACKGROUND: Inflammation is a key factor in the pathogenesis of bronchopulmonary dysplasia (BPD). Tumor necrosis factor-stimulated protein 6 (TSG-6) is a glycoprotein that modulates inflammation. Here we tested the hypothesis that intra-tracheal (IT) administration of an adenovirus overexpressing TSG-6 (AdTSG-6) would decrease inflammation and restore lung structure in experimental BPD. METHODS: Newborn Sprague-Dawley rats exposed to normoxia (RA) or hyperoxia (85% O2 ) from postnatal day (P) 1-P14 were randomly assigned to receive IT AdTSG-6 or placebo (PL) on P3...
December 11, 2018: Pediatric Research
Xiaochuan Chen, Bo Yang, Jun Tian, Hong Hong, Yu Du, Kun Li, Xin Li, Nan Wang, Xiaoqi Yu, Xi Wei
BACKGROUND/AIMS: Increasing evidence has demonstrated the novel roles of mesenchymal stem cells (MSCs) in immunotherapy. However, difficulty in acquiring these cells and possible ethical issues limited their application. Recently, we have isolated a unique MSC population from dental follicles with potent stem cell-like properties. This study focused on the effects of dental follicle stem cells (DFSCs) on macrophage activation and polarization to determine their role in immunomodulation and to test if DFSCs are a promising cell source for MSC-based immunotherapy...
2018: Cellular Physiology and Biochemistry
Moureq R Alotaibi, Amal J Fatani, Ahmed T Almnaizel, Mohammed M Ahmed, Hatem M Abuohashish, Salim S Al-Rejaie
OBJECTIVE: Diabetic complications involve multiple pathological pathways, including hyperglycemia-induced oxidative stress and inflammation. Combination therapy is usually employed to improve treatment outcomes and to lower potential adverse effects. In this study, we evaluated the effects of antidiabetic and antihypertensive agents, glibenclamide (GLI) and losartan (LT), on diabetes-associated metabolic changes in rats. MATERIALS AND METHODS: Streptozotocin (STZ)-induced diabetic animals were orally treated with GLI and/or LT 5 and 25 mg/kg respectively for 4 weeks...
December 10, 2018: Medical Principles and Practice: International Journal of the Kuwait University, Health Science Centre
Jae-Yeong Heo, Dong-Soon Im
Salvia miltiorrhiza root has been used in Asian traditional medicine for the treatment of cardiovascular diseases, asthma, and other conditions. Salvianolic acid B from S. miltiorrhiza extracts has been shown to improve airway hyperresponsiveness. We investigated the effects of salvianolic acid A, tanshinone I, and tanshinone IIA from S. miltiorrhiza in allergic asthma by using rat RBL-2H3 mast cells and female Balb/c mice. Antigen-induced degranulation was assessed by measuring β-hexosaminidase activity in vitro...
December 8, 2018: International Immunopharmacology
Elizabeth Klingbeil, Claire B de La Serre
There is accumulating evidence that the gut microbiota and its composition dynamics play a crucial role in regulating the host physiological functions and behavior. Diet composition is the primary modulator of bacterial richness and abundance in the gastrointestinal (GI) tract. Macronutrient (fat, sugar, and protein) and fiber contents are especially important in determining microbiota composition and its effect on health outcomes and behavior. In addition to food composition, time of intake and eating patterns have recently been shown to significantly affect gut bacterial makeup...
December 1, 2018: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
C-N Yang, S-H Kok, H-W Wang, J Z-C Chang, E H-H Lai, C-T Shun, H Yang, M-H Chen, C-Y Hong, S-K Lin
AIM: To assess the connection between mitophagy and hypoxia-induced apoptosis in osteoblasts and whether simvastatin alleviates bone resorption in apical periodontitis through modulation of mitophagy-related apoptosis. METHODOLOGY: Hypoxia-induced generation of reactive oxygen species in mitochondria and changes in mitochondrial membrane potential were evaluated respectively by MitoSOX and JC-1 fluorescence dye signaling. Accumulation of mitophagy markers PTEN-induced putative kinase 1 (PINK1) and Parkin in mitochondria was examined by Western blotting and immunofluorescence microscopy...
December 11, 2018: International Endodontic Journal
Nashwa Fathy Gamal El-Tahawy, Sara Mohamed Naguib Abdel Hafez, Maggie M Ramzy, Nagwa M Zenhom, Heba A Abdel-Hamid
Cerebellum seems to be a specific target for both the decrease of estrogen and hypertension in menopause. The aim of this study was to investigate the hypertension and menopause-induced changes in rat's cerebellar cortex and the possible mechanisms of these changes. Rats were divided into four groups: the sham-operated control (SC-group), the ovariectomized (OVX-group), the hypertensive (H-group), and the ovariectomized-hypertensive (OVX-H-group) group. The mean arterial pressure (MAP), serum nitric oxide (NO), lipid peroxides and antioxidant catalase enzyme levels were assayed...
December 7, 2018: Journal of Cellular Physiology
Nihat Laçin, Bozan Serhat İzol, Mehmet Cudi Tuncer, Ebru Gökalp Özkorkmaz, Buşra Deveci, Engin Deveci
BACKGROUND: The objective of this study was to investigate if long term formaldehyde inhalation may effect periodontal membrane and alveolar bone loss leading to periodontitis. the negative effects of formaldehyde was described using vascular endothelial growth factor (VEGF), matrix metallopeptidase 2 (MMP2) and Osteonectin antibodies involved in the extracellular matrix and angiogenetic development. MATERIALS AND METHODS: Thirty adult Wistar albino rats were used in this study...
December 11, 2018: Folia Morphologica (Warsz)
Y-M Li, Y Jiang, S-D Feng, Z-H Lin
OBJECTIVE: Infection can be caused by severe burnt, trauma and hypoxia, further causing systemic inflammatory syndrome or sepsis. The sepsis occurs in about 2% of all hospitalizations and ranges from 6% to 30% in intensive care unit (ICU) in developed countries This study aimed to investigate the effects of normal lymph fluid on sepsis complicated with pulmonary injury. MATERIALS AND METHODS: Wistar rats were prepared for sepsis complicated with acute pulmonary model via cecal ligation and puncture, and received normal lymph fluid injection 60 min later...
November 2018: European Review for Medical and Pharmacological Sciences
Kate R Secombe, Imogen A Ball, Joseph Shirren, Anthony D Wignall, John Finnie, Dorothy Keefe, Francesca Avogadri-Connors, Elizabeth Olek, David Martin, Susan Moran, Joanne M Bowen
PURPOSE: Neratinib is an irreversible pan-ErbB tyrosine kinase inhibitor used for the extended adjuvant treatment of early-stage HER2-positive breast cancer. Its use is associated with the development of severe diarrhea in up to 40% of patients in the absence of proactive management. We previously developed a rat model of neratinib-induced diarrhea and found inflammation and anatomical disruption in the ileum and colon. Here we tested whether anti-diarrheal interventions, budesonide and colesevelam, can reduce neratinib-induced diarrhea and intestinal pathology...
December 10, 2018: Cancer Chemotherapy and Pharmacology
Mohamed M Abdel-Diam, Dalia H Samak, Yasser S El-Sayed, Lotfi Aleya, Saud Alarifi, Saad Alkahtani
The ubiquitous use of diazinon (DZN, an organophosphorus insecticide) has increased the probability of occupational, public, and the ecosystem exposure; these exposures are linked to negative health outcomes. The flavonoids curcumin (CUR) and quercetin (QUE) exert significant anti-inflammatory and antioxidant activities against toxicants, including insecticides. However, it is unclear whether their combination enhances these activities. Therefore, 40 albino rat were divided randomly into the CTR, DZN, CUR + DZN, QUE + DZN, and CUR + QUE + DZN groups, which are treated daily via gavage for 28 days...
December 8, 2018: Environmental Science and Pollution Research International
Yuji Takeda, Hironobu Asao, Ichiro Wakabayashi
Various complications of diabetes are induced by the augmentation of chronic inflammation and attenuation of immunity. Leukocytes, which play major roles in inflammation and immune responses, are affected by the glycemic status and blood insulin level. In this chapter, we explain a method for analyzing the signal transduction pathway of leukocytes in peripheral blood. This method using flow cytometry can analyze a small amount of blood (50-100 μL/sample) without leukocyte purification. Thus, this procedure is useful for experiments using small-animal models of diabetes, such as mice and rats...
2019: Methods in Molecular Biology
Pooja Acharya, Ramaprasad Ravichandra Talahalli
Effect of aging and hyperglycemia on oxidative stress (OS) and inflammation in dyslipidemic conditions has not been elucidated. Hence, in this study, we assessed the implications of aging, hyperglycemia, and also the dietary effect of n-3 fatty acids (α-linolenic acid (ALA) and eicosapentaenoic acid (EPA) + docosahexaenoic acid (DHA)) on OS and inflammation in dyslipidemic rats. Dyslipidemia was induced in young and aged rats by feeding high-fat lard (HFL) diet. Diabetes was induced in young dyslipidemic rats by administering streptozotocin 30 days after the induction of dyslipidemia...
December 10, 2018: Inflammation
Hong Sun, Xinyu Shao, Jiajia He, Michal Golos, Bimin Shi
Since obesity is largely responsible for the growing incidence of renal tubulointerstitial inflammation, exploration into the mechanisms of obesity‑associated tubulointerstitial inflammation is essential. Studies have demonstrated that mammalian target of rapamycin (mTOR) is a crucial molecule in the pathogenesis of renal inflammation, including regulating the expression of inflammatory factors. The purpose of the present study was to further elucidate the role of mTOR in obesity‑associated tubulointerstitial inflammation...
December 5, 2018: Molecular Medicine Reports
Nicolas Esquerre, Lilian Basso, Caroline Dubuquoy, Madjid Djouina, Daniel Chappard, Catherine Blanpied, Pierre Desreumaux, Nathalie Vergnolle, Cécile Vignal, Mathilde Body-Malapel
Background & Aims: Irritable bowel syndrome (IBS) is a multifactorial disease arising from a complex interplay between genetic predisposition and environmental influences. To date, environmental triggers are not well known. Aluminum is commonly present in food, notably by its use as food additive. We investigated the effects of aluminum ingestion in rodent models of visceral hypersensitivity, and the mechanisms involved. Methods: Visceral hypersensitivity was recorded by colorectal distension in rats administered with oral low doses of aluminum...
2019: Cellular and Molecular Gastroenterology and Hepatology
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