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Neuropathic pain rats

Meng Wei, Lin Li, Yang Zhang, Zhi-Jie Zhang, Hai-Lin Liu, Hong-Guang Bao
Noncoding RNAs, including long non-coding RNAs (lncRNAs) and microRNAs, are involved in the development of neuropathic pain. Currently, we investigated that lncRNA X inactive-specific transcript (XIST) and toll-like receptor 5 (TLR5) were greatly upregulated in chronic constriction injury rat models, whereas miR-154-5p (microRNA-154-5p) was significantly downregulated. Bioinformatics analysis was used to predict miR-154-5p as a target gene of XIST, and dual-luciferase reporter tests proved the correlation between them...
October 18, 2018: Journal of Cellular Biochemistry
Georgios Baskozos, John M Dawes, Jean S Austin, Ana Antunes-Martins, Lucy McDermott, Alex J Clark, Teodora Trendafilova, Jon G Lees, Stephen B McMahon, Jeffrey S Mogil, Christine Orengo, David L Bennett
Dorsal root ganglion (DRG) neurons provide connectivity between peripheral tissues and spinal cord. Transcriptional plasticity within DRG sensory neurons after peripheral nerve injury contributes to nerve repair but also leads to maladaptive plasticity, including the development of neuropathic pain. This study presents tissue and neuron specific expression profiling of both known and novel Long Non-Coding RNAs (LncRNAs) in rodent DRG following nerve injury. We have identified a large number of novel LncRNAs expressed within rodent DRG, a minority of which were syntenically conserved between mouse, rat and human and which including both- intergenic and antisense LncRNAs...
October 16, 2018: Pain
Yujie Wang, Yu Zhao, Xiaohui Ma, Jing Li, Junling Hou, Xiaohong Lv
The purpose of this study was to examine the beneficial effects of electroacupuncture (EA) on neuropathic pain evoked by spinal cord injury (SCI) and determine the underlying molecular mechanisms of these effects. SCI was induced in rats. Behavioral tests were performed to examine pain responses induced by mechanical and thermal stimulation. Western blot analysis was used to measure the protein expression of phosphorylated mammalian target of rapamycin (p-mTOR), mTOR-mediated phosphorylated ribosomal protein S6 kinase beta-1 (p-S6K1), and phosphorylated eukaryotic translation initiation factor 4E-binding protein 1 (p-4E-BP1) in the superficial dorsal horn of the spinal cord...
October 17, 2018: Biological Research for Nursing
Jixiang Zhang, Shao-Rui Chen, Hong Chen, Hui-Lin Pan
Neuropathic pain is associated with persistent changes in gene expression in primary sensory neurons, but the underlying epigenetic mechanisms that cause these changes remain unclear. The muscarinic cholinergic receptors (mAChRs), particularly the M2 subtype (encoded by the cholinergic receptor muscarinic 2 [Chrm2] gene), are critically involved in the regulation of spinal nociceptive transmission. However, little is known about how Chrm2 expression is transcriptionally regulated. Here we show that nerve injury persistently increased the expression of RE1-silencing transcription factor (REST, also known as neuron-restrictive silencing factor [NRSF]), a gene-silencing transcription factor, in the dorsal root ganglion (DRG)...
October 16, 2018: Journal of Biological Chemistry
Xiaoli Zhang, Nan Jiang, Jing Li, Dongyan Zhang, Xiaohong Lv
BACKGROUND/AIMS: Paclitaxel is largely used as a chemotherapeutic agent for the treatment of several types of cancers. However, one of the significant limiting complications of paclitaxel is painful peripheral neuropathy during its therapy. The purposes of this study were to examine (1) the effects of blocking mammalian target of rapamycin (mTOR) on mechanical and thermal hypersensitivity evoked by paclitaxel; and (2) the underlying mechanisms responsible for the role of mTOR in regulating paclitaxel-induced neuropathic pain...
October 16, 2018: Neurological Research
Yong Fang Zhu, Jacek M Kwiecien, Wojciech Dabrowski, Robert Ungard, Kan Lun Zhu, Jan D Huizinga, James L Henry, Gurmit Singh
Evidence suggests that there are both nociceptive and neuropathic components of cancer-induced pain (CIP). We have observed that changes in intrinsic membrane properties and excitability of normally non-nociceptive Aβ sensory neurons are consistent in rat models of peripheral neuropathic pain (NEP) and CIP. This has prompted a comparative investigation of the intracellular electrophysiological characteristics of sensory neurons and of the ultrastructural morphology of the dorsal horn in rat models of NEP and CIP...
October 16, 2018: Molecular Pain
Xiaohui Ma, Sitong Liu, Dongxue Liu, Qian Wang, Hongwei Li, Zhen Zhao
This study was to examine the role of exercise intervention in modulating neuropathic pain induced by diabetes. Diabetes was induced by streptozotocin (STZ, i.p.) in rats and mechanical hyperalgesia was observed three weeks after STZ. Mechanical withdrawal thresholds were increased after four to five weeks of exercise in STZ rats. We also examined the role of signal of mammalian target of rapamycin (mTOR) in regulating neuropathic pain. Inhibition of neuropathic pain by exercise in STZ rats was accompanied with decreases of p-mTOR, p-S6K1, and p-4E-BP1 in sensory nerves...
October 13, 2018: Archives of Physiology and Biochemistry
Chengwei Peng, Chuanqing Zhang, Zhe Su, Dongyang Lin
Neuropathic pain caused by somatosensory nervous system dysfunction is a serious public health problem. Some long noncoding RNAs (lncRNAs) can participate in physiological processes involved in neuropathic pain. However, the effects of lncRNA DGCR5 in neuropathic pain have not been explored. Therefore, in our current study, we concentrated on the biological roles of DGCR5 in neuropathic pain. Here, it was observed that DGCR5 was significantly decreased in chronic sciatic nerve injury (CCI) rat models. DGCR5 overexpression was able to alleviate neuropathic pain development including mechanical and thermal hyperalgesia...
October 14, 2018: Journal of Cellular Physiology
Amber L Harris Bozer, Ai-Ling Li, Perry N Fuchs, Yuan B Peng
BACKGROUND: Neuropathic pain patients have described experiencing unprovoked, intermittent pain attacks with shooting, stabbing, and burning qualities. Rodent models used in previous literature usually only involve acute exposure, and/or are unable to manipulate the stimulation intensity in vivo by the experimenter during an experiment. NEW METHOD: This paper describes a method to induce controllable pain behaviors in rodents using a wireless portable electronic device that can be manipulated within the course of an experiment...
October 10, 2018: Journal of Neuroscience Methods
Chih-Peng Lin, Dai-Hua Lu
Opioid analgesics remain the most effective and widely used analgesics for the management of moderate to severe pain, including cancer pain and chronic non-cancer pain. However, the efficacy of long-term opioid analgesics is attenuated by tolerance and/or hyperalgesia after long-term use, preventing adequate pain relief under stable opioid dosages for chronic pain patients. Classical neuron-centered concepts about tolerance, such as internalization of opioid receptors, upregulation of N-methyl-D-aspartate receptor function, or downregulation of glutamate transporter activity, can only partially explain the phenomenon of tolerance...
2018: Advances in Experimental Medicine and Biology
Xiao-Qiu Ma, Jing Qin, Hong-Yan Li, Xiu-Li Yan, Yong Zhao, Lian-Jie Zhang
Hyperalgesia and allodynia are commonly observed in patients with diabetic neuropathy. The treatment and management of painful peripheral neuropathy is important in these patients. The purpose of this study was to examine the role of exercise in modulating neuropathic pain induced by diabetes. Diabetes was induced in rats by an intraperitoneal injection of streptozotocin (STZ). Control rats received saline injections. Groups included control rats without exercise (NT-control, n = 12), control rats with exercise (EX-control, n = 16), STZ rats without exercise (NT-STZ, n = 18), and STZ rats with exercise (EX-STZ, n = 22)...
October 10, 2018: Biological Research for Nursing
Hyung Gon Lee, Ji A Song, Dong Soo Han, Kyeong Wan Woo, Myung Ha Yoon
This study examined the effects of intrathecal areca nut on spinal nerve-ligated and chemotherapy-induced neuropathic pain (NP), and investigated the relevance of spinal 5-hydroxytryptamine (5-HT) and α2-adrenergic receptors to those effects. For drug administration, intrathecal catheters were inserted into the subarachnoid space of male Sprague-Dawley rats. NP was induced either by spinal nerve ligation (left spinal nerves L5 and L6) or by chemotherapeutic injection (intraperitoneal cisplatin, 2 mg/kg/day, once daily for 4 days)...
October 10, 2018: Pharmacology
Yiting Yin, Xin Qi, Yuan Qiao, Huaxiang Liu, Zihan Yan, Hao Li, Zhen Liu
The notion that proteasome inhibitor bortezomib (BTZ) induced intracellular oxidative stress resulting in peripheral neuropathy has been generally accepted. The association of mitochondrial dysfunction, cell apoptosis, and endoplasmic reticulum (ER) stress with intracellular oxidative stress is ambiguous and still needs to be investigated. Here we report a mechanism underlying the action of BTZ-induced intracellular oxidative stress, mitochondrial dysfunction, cell apoptosis, and ER stress via the activation of activating transcription factor 3 (ATF3) in primary cultured dorsal root ganglion (DRG) neurons and in DRG from painful peripheral neuropathic rats...
October 3, 2018: Current Cancer Drug Targets
Liang Wang, Tianyou Luo, Zhihua Bao, Yuan Li, WenJin Bu
Neuropathic pain is one of the most common diabetic complications and significantly decrease the quality of life. The aetiology of the painful diabetic neuropathic pain is not fully clear. Circular RNAs (circRNAs) have been identified as miRNA sponges and involved in various biological processes, including pain. CircHIPK3 is a circRNA that have been shown to be an oncogene or tumor suppressor to regulate cancer cells growth by sponging multiple miRNAs. However, the role of circHIPK3 in diabetic neuropathic pain remains unknown...
October 1, 2018: Biochemical and Biophysical Research Communications
Tzu-Hao Harry Chao, Jyh-Horng Chen, Chen-Tung Yen
Neuropathic pain is a major worldwide health problem. Although central sensitization has been reported in well-established neuropathic conditions, information on the acute brain activation patterns in response to peripheral nerve injury is lacking. This study first mapped the brain activity in rats immediately following spared nerve injury (SNI) of the sciatic nerve. Using blood-oxygenation-level-dependent functional magnetic resonance imaging (BOLD-fMRI), we observed sustained activation in the bilateral insular cortices (ICs), primary somatosensory cortex (S1), and cingulate cortex...
October 1, 2018: Molecular Brain
Guo-Fang Lu, Jun-Ming Zhang, Jian-Xiong An, Xiao-Yan Oian, Yong Wang, Si-Yu Lin, John P Williams
BACKGROUND: Electroacupuncture (EA) has been proved to be effective in treating certain neuropathic pain conditions. The mechanisms of pain relief by EA are not fully understood. There have been sporadic reports of damage in the peripheral nervous system (PNS) and regions of the central nervous system (CNS) at the ultrastructural level following peripheral nerve injury. However, information about possible systemic changes in the PNS and CNS after nerve injury is scarce. OBJECTIVES: The goal of this study was to examine the ultrastructural changes of the nervous system induced by a local injection of cobra venom into the sciatic nerve and to compare the ultrastructural changes in rats with or without treatment with EA or pregabalin...
September 2018: Pain Physician
Hai-Feng Lu, Chun-Yang Xu, Lei Zhang, Lei Gan, Chan Chen, Man-Yun Yan, Xiao-Ning Guo, Qi Fang, Guang-Yin Xu, Yan-Bo Zhang, Jian-Qiang Ni, Hong-Ru Zhao
Stroke is the leading cause of disability and death in the world. Central post-stroke pain (CPSP), a central neuropathic pain syndrome occurring after cerebral stroke, is a serious problem. But on account of the lack of reliable animal models, the mechanisms underlying CPSP remains poorly understood. To better understand of the pathophysiological basis of CPSP, we developed and characterized a new rat model of CPSP. This model is based on a hemorrhagic stroke lesion with intra-thalamic autologous blood (ITAB) injection in the ventral posterolateral nucleus of the thalamus...
September 26, 2018: Neuroscience Letters
Magda Descorbeth, Karen Figueroa, Miguel Serrano-Illán, Marino De León
BACKGROUND AND AIM: Docosahexaenoic acid (DHA) exhibits neuroprotective properties and has been shown to preserve nerve cells following trauma and ischemic injury. Recently, we showed that DHA pretreatment improved locomotion and reduced neuropathic pain after acute spinal cord injury in adult rats. These improvements were associated with an increase in the levels of AKT in spinal cord injury neurons. In this study, we investigate the implication of PI3K/AKT and mTOR pathway in DHA-mediated protection of primary cultured Schwann cells (pSC) undergoing palmitic acid-induced lipotoxicity (PA-LTx)...
September 28, 2018: Brain and Behavior
Ping Li, Dong-Lin Xiong, Wu-Ping Sun, Shi-Yuan Xu
Diabetic peripheral neuropathy is the most common complication of diabetes mellitus and leads to sensory symptoms, including diabetic neuropathic pain (DNP). DNP is a major public health problem because it has a considerable impact on life quality of diabetes mellitus patients. Therefore, development of novel effective analgesics for DNP relief and treatment is warranted. Transient receptor potential vanilloid 1 (TRPV1) has a crucial role in nociceptive transmission under pathological forms of pain. Baicalin is a flavonoid compound extracted from a medicinal herb, Huang Qin, it possesses antioxidant properties and has an analgesic effect on nitroglycerin-induced migraine in rats and neuropathic pain in spinal nerve ligation rats...
September 26, 2018: Neuroreport
Hui Du, Juan Shi, Ming Wang, Shuhong An, Xingjing Guo, Zhaojin Wang
BACKGROUND: Neuropathic pain is caused by damage to the nervous system, resulting in aberrant pain, which is associated with gene expression changes in the sensory pathway. However, the molecular mechanisms are not fully understood. METHODS: Wistar rats were employed for the establishment of the chronic constriction injury (CCI) models. Using the Illumina HiSeq 4000 platform, we examined differentially expressed genes (DEGs) in the rat dorsal horn by RNA sequencing (RNA-seq) between CCI and control groups...
September 25, 2018: Journal of Neuroinflammation
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