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https://www.readbyqxmd.com/read/29332277/efficient-degradation-of-triclosan-by-an-endophytic-fungus-penicillium-oxalicum-b4
#1
Hao Tian, Yan Jun Ma, Wan Yi Li, Jian Wen Wang
Triclosan (TCS), a widely used antimicrobial and preservative agent, is an emerging contaminant in aqueous and soil environment. Microbial degradation of TCS has not been reported frequently because of its inhibition of microbe growth. To explore the new microbial resources for TCS biodegradation, fungal endophytes were isolated and screened for the degradation potential. The endophytic strain B4 isolated from Artemisia annua L. showed higher degradation efficiency and was identified as Penicillium oxalicum based on its morphology and ITS sequences of ribosomal DNA...
January 13, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29329021/genotoxicity-analysis-of-five-particle-matter-toxicants-from-cigarette-smoke-based-on-%C3%AE-h2ax-assay-combined-with-hill-two-component-model
#2
Sen Zhang, Huan Chen, An Wang, Yong Liu, Hongwei Hou, Qingyuan Hu
To investigate the genotoxic characteristics of typical toxicants in particle phase of cigarette smoke, including B[a]P, nicotine, tar, NNN and NNK. The in vitro γH2AX assay was used to detect the DNA double-strand breaks (DSBs) in A549 cells using high content screening (HCS). The results showed all toxicants had a dose/time-dependent effects on induction of γH2AX except for NNN and NNK. Based on dose-response of γH2AX and Hill model, the ability to induce DSBs was evaluated: NNN-acetate > B[a]P > NNK-acetate > tar > nicotine...
January 5, 2018: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/29328473/detection-of-high-grade-neoplasia-in-air-dried-cervical-pap-smears-by-a-microrna-based-classifier
#3
Mikhail K Ivanov, Sergei E Titov, Sergei A Glushkov, Victoria V Dzyubenko, Anastasia V Malek, Polina A Arkhangelskaya, Roman B Samsonov, Andrey A Mikhetko, Elena V Bakhidze, Igor V Berlev, Nikolay N Kolesnikov
Recent studies have shown that changes in the expression levels of certain microRNAs correlate with the degree of severity of cervical lesions. The aim of the present study was to develop a microRNA-based classifier for the detection of high-grade cervical intraepithelial neoplasia (CIN ≥2) in cytological samples from patients with different high-risk human papillomavirus (HR-HPV) viral loads. For this purpose, raw RT-qPCR data for 25 candidate microRNAs, U6 snRNA and human DNA in air-dried PAP smears from 174 women with different cervical cytological diagnoses, 144 of which were HR-HPV-positive [40 negative for intraepithelial lesion or malignancy (NILM), 34 low-grade squamous intraepithelial lesions (L-SIL), 57 high-grade squamous intraepithelial lesions (H-SIL), 43 invasive cancers], were statistically processed...
January 12, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29327641/generation-of-an-arrayed-crispr-cas9-library-targeting-epigenetic-regulators-from-high-content-screens-to-in-vivo-assays
#4
Tristan Henser-Brownhill, Josep Monserrat, Paola Scaffidi
The CRISPR-Cas9 system has revolutionized genome engineering, allowing precise modification of DNA in various organisms. The most popular method for conducting CRISPR-based functional screens involves the use of pooled lentiviral libraries in selection screens coupled with next-generation sequencing. Screens employing genome-scale pooled small guide RNA (sgRNA) libraries are demanding, particularly when complex assays are used. Furthermore, pooled libraries are not suitable for microscopy-based high-content screens or for systematic interrogation of protein function...
January 12, 2018: Epigenetics: Official Journal of the DNA Methylation Society
https://www.readbyqxmd.com/read/29324561/nursing-minimum-data-sets-for-documenting-nutritional-care-for-adults-in-primary-healthcare-a-scoping-review
#5
Sasja Jul Håkonsen, Preben Ulrich Pedersen, Merete Bjerrum, Ann Bygholm, Micah D J Peters
OBJECTIVE: To identify all published nutritional screening instruments that have been validated in the adult population in primary healthcare settings and to report on their psychometric validity. INTRODUCTION: Within health care, there is an urgent need for the systematic collection of nursing care data in order to make visible what nurses do and to facilitate comparison, quality assurance, management, research and funding of nursing care. To be effective, nursing records should accurately and comprehensively document all required information to support safe and high quality care of patients...
January 2018: JBI Database of Systematic Reviews and Implementation Reports
https://www.readbyqxmd.com/read/29324414/no-reference-quality-assessment-for-screen-content-images-with-both-local-and-global-feature-representation
#6
Yuming Fang, Jiebin Yan, Leida Li, Jinjian Wu, Weisi Lin
In this paper, we propose a novel no reference quality assessment method by incorporating statistical luminance and texture features (NRLT) for screen content images (SCIs) with both local and global feature representation. The proposed method is designed inspired by the perceptual property of the human visual system (HVS) that the HVS is sensitive to luminance change and texture information for image perception. In the proposed method, we first calculate the luminance map through the local normalization, which is further used to extract the statistical luminance features in global scope...
April 2018: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/29320234/influence-of-soap-characteristics-and-food-service-facility-type-on-the-degree-of-bacterial-contamination-of-open-refillable-bulk-soaps
#7
Donald W Schaffner, Dane Jensen, Charles P Gerba, David Shumaker, James W Arbogast
Concern has been raised regarding the public health risks from refillable bulk-soap dispensers because they provide an environment for potentially pathogenic bacteria to grow. This study surveyed the microbial quality of open refillable bulk soap in four different food establishment types in three states. Two hundred ninety-six samples of bulk soap were collected from food service establishments in Arizona, New Jersey, and Ohio. Samples were tested for total heterotrophic viable bacteria, Pseudomonas, coliforms and Escherichia coli, and Salmonella...
January 10, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29319097/conformational-stabilization-of-a-%C3%AE-hairpin-through-a-triazole-tryptophan-interaction
#8
Donatella Diana, Claudia Di Salvo, Veronica Celentano, Lucia De Rosa, Alessandra Romanelli, Roberto Fattorusso, Luca D D'Andrea
Molecular tools to stabilize the β-hairpin conformation are needed as β-hairpin peptides are useful molecules for pharmaceutical, biological and materials applications. We explored the use of a "triazole bridge", a covalent link between two β-hairpin strands obtained through Cu-catalyzed alkyne-azide cycloaddition, combined with an aromatic-aromatic interaction. Highly conformationally stable peptides were identified by NMR screening of a small collection of cyclic peptides based on the Trpzip2 scaffold...
January 10, 2018: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/29311274/mutations-in-escherichia-coli-polyphosphate-kinase-that-lead-to-dramatically-increased-in-vivo-polyphosphate-levels
#9
Amanda K Rudat, Arya Pokhrel, Todd J Green, Michael J Gray
Bacteria synthesize inorganic polyphosphate (polyP) in response to a wide variety of stresses, and production of polyP is essential for stress response and survival in many important pathogens and bacteria used in biotechnological processes. However, surprisingly little is known about the molecular mechanisms that control polyP synthesis. We have therefore developed a novel genetic screen that specifically links growth of Escherichia coli to polyP synthesis, allowing us to isolate mutations leading to enhanced polyP production...
January 8, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29311064/high-content-screening-of-mmv-pathogen-box-for-plasmodium-falciparum-digestive-vacuole-disrupting-molecules-reveals-valuable-starting-points-for-drug-discovery
#10
Jie Xin Tong, Rajesh Chandramohanadas, Kevin Shyong-Wei Tan
Plasmodium falciparum infections leading to malaria have severe clinical manifestations and high mortality rates. Chloroquine (CQ), a former mainstay of malaria chemotherapy, has been rendered ineffective due to the emergence of wide-spread resistance. Recent studies, however, have unveiled a novel mode of action in which low micromolar levels of CQ permeabilized the parasite's digestive vacuole (DV) membrane, leading to calcium efflux, mitochondrial depolarization and DNA degradation. These phenotypes implicate the DV as an alternative target of CQ and suggests that DV disruption is an attractive target for exploitation through the screening for DV-disruptive antimalarials...
January 8, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29310218/raman-hyperspectral-imaging-as-an-effective-and-highly-informative-tool-to-study-the-diagenetic-alteration-of-fossil-bones
#11
Gregorio Dal Sasso, Ivana Angelini, Lara Maritan, Gilberto Artioli
Retrieving the pristine chemical or isotopic composition of archaeological bones is of great interest for many studies aiming to reconstruct the past life of ancient populations (i.e. diet, mobility, palaeoenvironment, age). However, from the death of the individual onwards, bones undergo several taphonomic and diagenetic processes that cause the alteration of their microstructure and composition. A detailed study on bone diagenesis has the double purpose to assess the preservation state of archaeological bones and to understand the alteration pathways, thus providing evidence that may contribute to evaluate the reliability of the retrieved information...
March 1, 2018: Talanta
https://www.readbyqxmd.com/read/29310013/bioaccumulation-and-physiological-responses-to-lead-pb-in-chenopodium-murale-l
#12
Gagan Preet Singh Sidhu, Aditi Shreeya Bali, Renu Bhardwaj, Harminder Pal Singh, Daizy R Batish, Ravinder Kumar Kohli
Identification and screening of wild, wasteland plants for the remediation of heavy metal contaminated soils is a crucial phytotechnological approach. In a pot experiment, Chenopodium murale was tested for the phytoextraction of lead (Pb) from the contaminated soils. The experiment was conducted for 8 weeks to appraise the effect of varied Pb regimes (300, 400, 500mgkg-1) on the bioaccumulation and physiological responses of C. murale plants. At 500mgkg-1 Pb regime, the concentration of Pb in roots and shoots reached a maximum of 2513 and 2301mgkg-1 DW, respectively...
January 5, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29309766/heme-oxygnease-1-induction-by-methylene-blue-protects-raw264-7-cells-from-hydrogen-peroxide-induced-injury
#13
Xiao-Tong Zhang, Xue-Qiang Sun, Chen Wu, Jun-Liang Chen, Jia-Jia Yuan, Qing-Feng Pang, Zhi-Ping Wang
Althoughmethylene blue (MB) has showed strong antioxidant effect, its effect related with heme oxygenase-1(HO-1) is still unclear. Thus, we investigated the effects of MB on HO-1 protein content and enzyme activity, and its protective effect against hydrogen peroxide (H2O2)- induced oxidative damage in RAW264.7 macrophage. The cellviability and the release of lactate dehydrogenase of RAW264.7 were determined. The mitochondrial functions were valuated through these indexes: content of adenosine triphosphate, superoxide dismutase, concentration of reactive oxygen species and mitochondrial membrane potential...
January 5, 2018: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29307837/development-of-a-whole-cell-based-screening-method-for-a-carotenoid-assay-using-aerial-microalgae
#14
Nobuhiro Aburai, Hiroaki Kazama, Atsushi Tsuruoka, Mizuki Goto, Katsuya Abe
Non-destructive approaches based on the application of optical spectroscopy are important for monitoring carotenoid accumulation in a whole cell cultured under various conditions. A simple and rapid assay utilizing aerial microalgae helps to identify stress conditions that can efficiently enhance the carotenogenesis in photosynthetic organisms. The spectra of cell suspensions were characterized in the aerial microalga Coelastrella sp. KGU-Y002, which are unicellular and undifferentiated. Total carotenoid contents could be successfully estimated on the basis of the absorbance values of the cell suspensions and calibration data analyzed by HPLC (high-performance liquid chromatography)...
January 4, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29307391/variables-associated-with-mitochondrial-copy-number-in-human-blastocysts-what-can-we-learn-from-trophectoderm-biopsies
#15
Maria José de Los Santos, Antonio Diez Juan, Amparo Mifsud, Amparo Mercader, Marcos Meseguer, Carmen Rubio, Antonio Pellicer
OBJECTIVE: To study the potential variables that affect the mitochondrial DNA (mtDNA) content of trophectoderm (TE) cells in blastocysts that have undergone TE biopsy. DESIGN: Observational retrospective single-center analysis. SETTING: University-affiliated private in vitro fertilization center. PATIENT(S): A total of 465 consecutive preimplantation genetic screening (PGS) cycles of 402 women undergoing preimplantation genetic testing...
January 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29307188/polymorphism-of-indomethacin-in-semicrystalline-dispersions-formation-transformation-and-segregation
#16
Tu Van Duong, David Ludeker, Pieter-Jan Van Bockstal, Thomas De Beer, Jan Van Humbeeck, Guy Van den Mooter
The crystallization of metastable crystal polymorphs in polymer matrices has been extensively reported in literature as a possible approach to enhance the solubility of poorly water soluble drug compounds, yet no clarification of the mechanism of the polymorph formation was proposed. The current work aims to elucidate the polymorphism behavior of the model compound indomethacin as well as the mechanism of polymorph selection of drugs in semicrystalline systems. Indomethacin crystallized as either the α or τ form - a new metastable form, or a mixture of the two polymorphs in dispersions containing different drug loadings in polyethylene glycol, poloxamer or Gelucire as the result of the variation in the mobility of drug molecules...
January 8, 2018: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/29303003/development-and-content-validity-of-the-behavioral-assessment-screening-tool-bast%C3%AE
#17
Shannon B Juengst, Lauren Terhorst, Brad E Dicianno, Janet P Niemeier, Amy K Wagner
PURPOSE: Develop and establish the content validity of the Behavioral Assessment Screening Tool (BASTβ), a self-reported measure of behavioral and emotional symptoms after traumatic brain injury. METHODS: This was an assessment development study, including two focus groups of individuals with traumatic brain injury (n = 11) and their family members (n = 10) and an expert panel evaluation of content validity by experts in traumatic brain injury rehabilitation (n = 7)...
January 5, 2018: Disability and Rehabilitation
https://www.readbyqxmd.com/read/29301342/small-myristoylated-protein-3-identified-as-a-potential-virulence-factor-in-leishmania-amazonensis-proves-to-be-a-protective-antigen-against-visceral-leishmaniasis
#18
Marcelo P Oliveira, Vívian T Martins, Thaís T O Santos, Daniela P Lage, Fernanda F Ramos, Beatriz C S Salles, Lourena E Costa, Daniel S Dias, Patrícia A F Ribeiro, Mônica S Schneider, Ricardo A Machado-de-Ávila, Antônio L Teixeira, Eduardo A F Coelho, Miguel A Chávez-Fumagalli
In a proteomics approach conducted with Leishmania amazonensis, parasite proteins showed either an increase or a decrease in their expression content during extensive in vitro cultivation, and were related to the survival and the infectivity of the parasites, respectively. In the current study, a computational screening was performed to predict virulence factors among these molecules. Three proteins were selected, one of which presented no homology to human proteins. This candidate, namely small myristoylated protein-3 (SMP-3), was cloned, and its recombinant version (rSMP-3) was used to stimulate peripheral blood mononuclear cells (PBMCs) from healthy subjects living in an endemic area of leishmaniasis and from visceral leishmaniasis patients...
January 3, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29299815/older-adults-perceptions-of-the-causes-and-consequences-of-healthcare-overuse-a-qualitative-study
#19
Ariel R Green, Monica Tung, Jodi B Segal
BACKGROUND: Overuse of healthcare is pervasive in the United States, often exposing patients to harm with little likelihood of benefit. Older Americans are particularly vulnerable to overuse and impacted by it, yet it is unknown whether older patients perceive overuse as a consequential problem. OBJECTIVE: To explore the experiences and perspectives of older adults with respect to healthcare overuse in order to develop a framework for understanding and reducing overuse in older adults...
January 3, 2018: Journal of General Internal Medicine
https://www.readbyqxmd.com/read/29299633/inadequacy-of-conventional-grab-sampling-for-remediation-decision-making-for-metal-contamination-at-small-arms-ranges
#20
J L Clausen, T Georgian, K H Gardner, T A Douglas
Research shows grab sampling is inadequate for evaluating military ranges contaminated with energetics because of their highly heterogeneous distribution. Similar studies assessing the heterogeneous distribution of metals at small-arms ranges (SAR) are lacking. To address this we evaluated whether grab sampling provides appropriate data for performing risk analysis at metal-contaminated SARs characterized with 30-48 grab samples. We evaluated the extractable metal content of Cu, Pb, Sb, and Zn of the field data using a Monte Carlo random resampling with replacement (bootstrapping) simulation approach...
January 3, 2018: Bulletin of Environmental Contamination and Toxicology
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