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High content screening

Andrew McDowell, Neeraj Raizada, Sunil D Khaparde, Raghuram Rao, Sanjay Sarin, Aakshi Kalra, Virender Singh Salhotra, Sreenivas Achuthan Nair, Catharina Boehme, Claudia M Denkinger
BACKGROUND: Diagnosing tuberculosis (TB) in children presents considerable challenges. Upfront testing on Xpert® MTB/RIF ('Xpert')-a rapid molecular assay with high sensitivity and specificity-for pediatric presumptive TB patients, as recommended by India's Revised National Tuberculosis Control Program (RNTCP), can pave the way for early TB diagnosis. As part of an ongoing project implemented by Foundation for Innovative New Diagnostics (FIND) dedicated to providing upfront free-of-cost (FOC) Xpert testing to children seeking care in the public and private sectors, a qualitative assessment was designed to understand how national guidelines on TB diagnosis and Xpert technology have been integrated into the pediatric TB care practices of different health providers...
2018: PloS One
Xin Deng, Wen Zhao, Laijun Song, Wei Ying, Xinbin Guo
Glioma is one of the most common aggressive neuroepithelial malignant tumors in the central nervous system. It has a high recurrence rate and poor prognosis, primarily due to the fact that novel therapeutic agents cannot penetrate the blood-brain barrier (BBB). Endothelial progenitor cells (EPCs) have been reported to move across the BBB and access the tumor site. However, whether EPCs expressing the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induce glioma cell apoptosis requires further investigation...
April 2018: Oncology Letters
Sara Moccia, Gabriele O Vanone, Elena De Momi, Andrea Laborai, Luca Guastini, Giorgio Peretti, Leonardo S Mattos
BACKGROUND AND OBJECTIVE: Early-stage diagnosis of laryngeal cancer is of primary importance to reduce patient morbidity. Narrow-band imaging (NBI) endoscopy is commonly used for screening purposes, reducing the risks linked to a biopsy but at the cost of some drawbacks, such as large amount of data to review to make the diagnosis. The purpose of this paper is to present a strategy to perform automatic selection of informative endoscopic video frames, which can reduce the amount of data to process and potentially increase diagnosis performance...
May 2018: Computer Methods and Programs in Biomedicine
Sajitha Biju, Sigfredo Fuentes, Dorin Gupta
Lentil (Lens culinaris, Medik.) is an important legume crop, which often experience drought stress especially at the flowering and grain filling phenological stages. The availability of efficient and robust screening tools based on relevant non-destructive quantifiable traits would facilitate research on crop improvement for drought tolerance. The objective of this study was to evaluate the drought tolerance of 37 lentil genotypes using infrared thermal imaging (IRTI), drought tolerance parameters and multivariate data analysis...
March 8, 2018: Plant Physiology and Biochemistry: PPB
Jia Wu, Adam Elliston, Gwenaelle Le Gall, Ian J Colquhoun, Samuel R A Collins, Ian P Wood, Jo Dicks, Ian N Roberts, Keith W Waldron
Background: Rice straw and husk are globally significant sources of cellulose-rich biomass and there is great interest in converting them to bioethanol. However, rice husk is reportedly much more recalcitrant than rice straw and produces larger quantities of fermentation inhibitors. The aim of this study was to explore the underlying differences between rice straw and rice husk with reference to the composition of the pre-treatment liquors and their impacts on saccharification and fermentation...
2018: Biotechnology for Biofuels
Matthew J Page, Joanne E McKenzie, Julian P T Higgins
BACKGROUND: Several scales, checklists and domain-based tools for assessing risk of reporting biases exist, but it is unclear how much they vary in content and guidance. We conducted a systematic review of the content and measurement properties of such tools. METHODS: We searched for potentially relevant articles in Ovid MEDLINE, Ovid Embase, Ovid PsycINFO and Google Scholar from inception to February 2017. One author screened all titles, abstracts and full text articles, and collected data on tool characteristics...
March 14, 2018: BMJ Open
Kengo Watanabe, Tsuyoshi Umeda, Kuniyoshi Niwa, Isao Naguro, Hidenori Ichijo
Cell volume regulation is a vital system for cellular activities. When perturbed by hypoosmotic or hyperosmotic stress, cells immediately induce the cell volume recovery system, regulatory volume decrease (RVD) or regulatory volume increase (RVI), respectively. In contrast to the knowledge about effector molecules, the molecular mechanisms linking osmosensing to RVD/RVI induction remain unknown. Additionally, few reciprocal responders in the bidirectional osmotic stress response have been identified. We previously reported that ASK3 bidirectionally switches its kinase activity under osmotic stress...
March 13, 2018: Cell Reports
Zhaohua Zhang, Xiangning Tang, Jun Li, Wenyi Yang
PURPOSE: To investigate the effect of cold stimulation (contacting wet fabric) and tactile stimulation (dynamic friction) on skin wetness perception: 1) wetness perception of fabric with different water transfer performance; 2) wetness perception of fabric with different water content; 3) wetness perception of fabric at different friction velocity with skin. METHODS: Twenty participants were screened as reliable assessors using a 7-point wetness rating scale. The dynamic friction between skin and fabric was simulated by a self-made device with adjustable speed controller...
March 14, 2018: International Journal of Occupational Safety and Ergonomics: JOSE
An Ohigashi, Salim Ahmed, Arfan R Afzal, Naoko Shigeta, Helen Tam-Tham, Hideyuki Kanda, Yoshihiro Ishikawa, Tanvir C Turin
INTRODUCTION Japan is a developed country with high use of Internet and online platforms for health information. 'Yahoo! Answer Japan' is the most commonly used question-and-answer service in Japan. AIM To explore the information users seek regarding breast cancer from the 'Yahoo! Answer Japan' web portal. METHODS The 'Yahoo! Answer Japan' portal was searched for the key word 'breast cancer' and all questions searched for the period of 1 January to 31 December 2014 were obtained. The selected questions related to human breast cancer and were not advertisements or promotional material...
June 2017: Journal of Primary Health Care
Constance A Mitchell, Subham Dasgupta, Sharon Zhang, Heather M Stapleton, David C Volz
Triphenyl phosphate (TPHP) is an unsubstituted aryl phosphate ester used as a flame retardant and plasticizer within the United States. Using zebrafish as a model, the objectives of this study were to rely on 1) mRNA-sequencing to uncover pathways disrupted following embryonic TPHP exposure and 2) high-content screening to identify nuclear receptor ligands that enhance or mitigate TPHP-induced cardiotoxicity. Based on mRNA-sequencing, TPHP exposure from 24 to 72 h post fertilization (hpf) resulted in a concentration-dependent increase in the number of transcripts significantly affected at 72 hpf, and pathway analysis revealed that five out of nine nuclear receptor pathways were associated with the retinoid X receptor (RXR)...
February 24, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
Kessarawan Nilvarangkul, Teerasak Phajan, WongsaLAOHASIRIWONG, John F Smith, Dariwan Settheetham
This cross sectional study developed and validated a LBP risk-factor screening scale for use with sugarcane farmers. The scale was developed from a synthesis of LBP risk factors, pretested with 30 sugarcane farmers and administered to five hundred and forty sugarcane farmers to test its psychometric properties. Results indicated construct validity for three factors; physical factors (19 items) with factor loadings of 0.406 to 0.881 and communalities between 0.471 and 0.991; psychological factors (7 items) with factor loadings of 0...
March 10, 2018: Industrial Health
Eniko Farkas, Andras Szekacs, Boglarka Kovacs, Marianna Olah, Robert Horvath, Inna Szekacs
Rapid and inexpensive biosensor technologies allowing real-time analysis of biomolecular and cellular events have become the basis of next-generation cell-based screening techniques. Our work opens up novel opportunities in the application of the high-throughput label-free Epic BenchTop optical biosensor in cell toxicity studies. The Epic technology records integrated cellular responses about changes in cell morphology and dynamic mass redistribution of cellular contents at the 100-150 nm layer above the sensor surface...
February 23, 2018: Journal of Hazardous Materials
Sara Ahmed, Dhanveer Singh, Shereen Khattab, Jessica Babineau, Dinesh Kumbhare
Background: There is an increasing trend in the consumption of poor-quality diets worldwide, contributing to the increase of non-communicable diseases. Diet directly influences physiological composition and subsequently physical health. Studies have shown that dietary macronutrient and energy content can influence the proportion of intramuscular fat (IMF), which mediates various metabolic and endocrine dysfunction. The purpose of this systematic review was to identify evidence in the literature assessing the association between different dietary interventions on the proportion of IMF in humans...
2018: Frontiers in Nutrition
Meinald T Thielsch, Carolin Thielsch
Background: Depression, as one of the most prevalent mental disorders, is expected to become a leading cause of disability. While evidence-based treatments are not always easily accessible, Internet-based information and self-help appears as a promising approach to improve the strained supply situation by avoiding barriers of traditional offline treatment. User experience in the domain of mental problems therefore emerges as an important research topic. The aim of our study is to investigate the impact of depressive symptoms on subjective and objective measures of web user experience...
2018: PeerJ
Seyed Hossein Mamanpush, Hui Li, Karl Englund, Azadeh Tavousi Tabatabaei
With an increase in renewable wind energy via turbines, an underlying problem of the turbine blade disposal is looming in many areas of the world. These wind turbine blades are predominately a mixture of glass fiber composites (GFCs) and wood and currently have not found an economically viable recycling pathway. This work investigates a series of second generation composites fabricated using recycled wind turbine material and a polyurethane adhesive. The recycled material was first comminuted via a hammer-mill through a range of varying screen sizes, resinated and compressed to a final thickness...
March 2, 2018: Waste Management
A S Malalagama, S Tennakoon, D R R Abeyasinghe
PURPOSE: Youth suicide rates are rising worldwide, including Sri Lanka, where 46.5/100,000 among 15-19yrs was reported in 1986. Identifying high risk adolescents is vital. Dearth of trained professionals, demands an instrument utilizable by non-psychiatrists. Such was not available in Sinhalese. Adolescent Suicide Assessment Protocol (ASAP-20) and its' manual was translated and validated to Sri Lankan adolescents. METHOD: A validation study. Forward/back translations followed by cultural adaptation...
February 24, 2018: Asian Journal of Psychiatry
Francesco Masia, Iestyn Pope, Peter Watson, Wolfgang Langbein, Paola Borri
Microscopy based high-content and high-throughput analysis of cellular systems plays a central role in drug discovery. However, for contrast and specificity, the majority of assays require a fluorescent readout which always comes with the risk of alteration of the true biological conditions. In this work, we demonstrate a label-free imaging platform which combines chemically-specific hyperspectral coherent anti-Stokes Raman scattering microscopy with sparse sampling and Bessel beam illumination. This enabled us to screen multi-well plates at high speed while retaining the high-content chemical analysis of hyperspectral imaging...
March 5, 2018: Analytical Chemistry
Bruno Briani, Marina N Sissini, Leidson A Lucena, Manuela B Batista, Iara O Costa, José M C Nunes, Caroline Schmitz, Fernanda Ramlov, Marcelo Maraschin, Nathalie Korbee, Leonardo Rörig, Paulo A Horta, Félix L Figueroa, José B Barufi
Mycosporine-like amino acids (MAA) are ultraviolet screen substances synthesized by marine algae. The physiological function of these substances is related to cellular protection against UV radiation and as a protective mechanism against oxidative stress. These substances can be found mainly in the ocean, among red seaweeds. Its concentration in organisms has been related to ultraviolet radiation and availability of inorganic nitrogen in the environment. We start our study of MAA content in different species to understand if environmental conditions influence the concentration of MAAs in red seaweeds...
March 5, 2018: Journal of Phycology
Jaak Simm, Günter Klambauer, Adam Arany, Marvin Steijaert, Jörg Kurt Wegner, Emmanuel Gustin, Vladimir Chupakhin, Yolanda T Chong, Jorge Vialard, Peter Buijnsters, Ingrid Velter, Alexander Vapirev, Shantanu Singh, Anne E Carpenter, Roel Wuyts, Sepp Hochreiter, Yves Moreau, Hugo Ceulemans
In both academia and the pharmaceutical industry, large-scale assays for drug discovery are expensive and often impractical, particularly for the increasingly important physiologically relevant model systems that require primary cells, organoids, whole organisms, or expensive or rare reagents. We hypothesized that data from a single high-throughput imaging assay can be repurposed to predict the biological activity of compounds in other assays, even those targeting alternate pathways or biological processes...
February 16, 2018: Cell Chemical Biology
Dalal Asker, Tarek S Awad, Teruhiko Beppu, Kenji Ueda
Ketocarotenoids are high-value natural pigments. The red diketocarotenoid astaxanthin particularly exhibits an extraordinary antioxidant activity, which raises its market demand for foods and nutraceuticals. We screened for ketocarotenoid-producing bacteria from both marine and freshwater environments. Phylogenetic analysis, based on 16S rRNA gene sequence, revealed 37 potential producers of ketocarotenoids that are related to α-proteobacteria, comprising 32 strains of Brevundimonas and 5 strains of Erythrobacter...
July 1, 2018: Food Chemistry
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