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High content screening

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https://www.readbyqxmd.com/read/28647475/advanced-cell-classifier-user-friendly-machine-learning-based-software-for-discovering-phenotypes-in-high-content-imaging-data
#1
Filippo Piccinini, Tamas Balassa, Abel Szkalisity, Csaba Molnar, Lassi Paavolainen, Kaisa Kujala, Krisztina Buzas, Marie Sarazova, Vilja Pietiainen, Ulrike Kutay, Kevin Smith, Peter Horvath
High-content, imaging-based screens now routinely generate data on a scale that precludes manual verification and interrogation. Software applying machine learning has become an essential tool to automate analysis, but these methods require annotated examples to learn from. Efficiently exploring large datasets to find relevant examples remains a challenging bottleneck. Here, we present Advanced Cell Classifier (ACC), a graphical software package for phenotypic analysis that addresses these difficulties. ACC applies machine-learning and image-analysis methods to high-content data generated by large-scale, cell-based experiments...
June 16, 2017: Cell Systems
https://www.readbyqxmd.com/read/28644962/validity-and-reproducibility-of-the-modified-start-back-tool-dutch-version-for-patients-with-neck-pain-in-primary-care
#2
Jasper D Bier, Raymond W J G Ostelo, Bart W Koes, Arianne P Verhagen
OBJECTIVE: To evaluate the reliability and validity of the Dutch version of the STarT Back screening Tool (SBT), for patients with neck pain. METHODS: We modified the SBT to fit patients with neck pain. General practitioners and physiotherapists included patients who completed both a baseline and a follow-up questionnaire at 3 days and 3 months, respectively. The construct validity was assessed using Pearson's correlation between the SBT and the reference questionnaires...
June 15, 2017: Musculoskeletal Science & Practice
https://www.readbyqxmd.com/read/28644808/esim-edge-similarity-for-screen-content-image-quality-assessment
#3
Zhangkai Ni, Lin Ma, Huanqiang Zeng, Jing Chen, Canhui Cai, Kai-Kuang Ma
In this paper, an accurate full-reference image quality assessment (IQA) model developed for assessing screen content images (SCIs), called the edge similarity (ESIM), is proposed. It is inspired by the fact that the human visual system (HVS) is highly sensitive to edges that are often encountered in SCIs; therefore, essential edge features are extracted and exploited for conducting IQA for the SCIs. The key novelty of the proposed ESIM lies in the extraction and use of three salient edge features-i.e., edge contrast, edge width, and edge direction...
June 21, 2017: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/28644037/lower-risk-cannabis-use-guidelines-a-comprehensive-update-of-evidence-and-recommendations
#4
Benedikt Fischer, Cayley Russell, Pamela Sabioni, Wim van den Brink, Bernard Le Foll, Wayne Hall, Jürgen Rehm, Robin Room
BACKGROUND: Cannabis use is common in North America, especially among young people, and is associated with a risk of various acute and chronic adverse health outcomes. Cannabis control regimes are evolving, for example toward a national legalization policy in Canada, with the aim to improve public health, and thus require evidence-based interventions. As cannabis-related health outcomes may be influenced by behaviors that are modifiable by the user, evidence-based Lower-Risk Cannabis Use Guidelines (LRCUG)-akin to similar guidelines in other health fields-offer a valuable, targeted prevention tool to improve public health outcomes...
June 23, 2017: American Journal of Public Health
https://www.readbyqxmd.com/read/28642722/revision-of-audit-consumption-items-to-improve-the-screening-of-youth-binge-drinking
#5
María-Teresa Cortés-Tomás, José-Antonio Giménez-Costa, Patricia Motos-Sellés, María-Dolores Sancerni-Beitia
This study analyzes the appropriateness of an improved version of one of the most frequently used instruments for the screening of high-risk alcohol consumption. This adaptation was created in accordance with certain limitations recognized by other researchers and in an attempt to adjust the content and scales of some items to a more consensual definition of binge drinking. After revising items 2 and 3, the areas under the ROC curves of the AUDIT and of different abbreviated versions were calculated. A total of 906 minors (468 females) between the ages of 15 and 17 were evaluated...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28634874/the-childcare-center-an-untapped-opportunity-to-engage-and-educate-families-in-healthy-behaviors
#6
Michelle M Cloutier, James F Wiley, Christine Trapp, Jennifer Haile, Amy A Gorin
Obesity rates in preschool children are high and disproportionately affect low-income children of color in the USA. Since 80% of preschool children spend ∼40 h/week in out-of-the home childcare, childcare centers are promising sites for obesity prevention interventions. Mixed methods were used to develop, implement, and assess the feasibility of an obesity prevention program for children 2-5 years. The intervention which consisted of brief (1-3 min), interactive, educational modules was developed by content experts and parents (n = 20) and targeted four areas (milk, sugar sweetened beverages, screen time, and physical activity)...
June 20, 2017: Journal of Racial and Ethnic Health Disparities
https://www.readbyqxmd.com/read/28633418/detecting-and-removing-multiplicative-spatial-bias-in-high-throughput-screening-technologies
#7
Iurie Caraus, Bogdan Mazoure, Robert Nadon, Vladimir Makarenkov
Motivation: Considerable attention has been paid recently to improve data quality in high-throughput screening (HTS) and high-content screening (HCS) technologies widely used in drug development and chemical toxicity research. However, several environmentally- and procedurally-induced spatial biases in these screens decrease measurement accuracy, leading to increased numbers of false positives and false negatives in hit selection. Although effective bias correction methods and software have been developed over the past decades, almost all of these tools have been designed to reduce the effect of additive bias only...
June 15, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28632767/how-is-indonesia-coping-with-its-epidemic-of-chronic-noncommunicable-diseases-a-systematic-review-with-meta-analysis
#8
Julia Schröders, Stig Wall, Mohammad Hakimi, Fatwa Sari Tetra Dewi, Lars Weinehall, Mark Nichter, Maria Nilsson, Hari Kusnanto, Ekowati Rahajeng, Nawi Ng
BACKGROUND: Chronic noncommunicable diseases (NCDs) have emerged as a huge global health problem in low- and middle-income countries. The magnitude of the rise of NCDs is particularly visible in Southeast Asia where limited resources have been used to address this rising epidemic, as in the case of Indonesia. Robust evidence to measure growing NCD-related burdens at national and local levels and to aid national discussion on social determinants of health and intra-country inequalities is needed...
2017: PloS One
https://www.readbyqxmd.com/read/28628525/assessing-sensitivity-and-specificity-of-the-manchester-triage-system-in-the-evaluation-of-acute-coronary-syndrome-in-adult-patients-in-emergency-care-a-systematic-review
#9
Fernanda Ayache Nishi, Flávia de Oliveira Motta Maia, Itamar de Souza Santos, Dina de Almeida Lopes Monteiro da Cruz
BACKGROUND: Triage is the first assessment and sorting process used to prioritize patients arriving in the emergency department (ED). As a triage tool, the Manchester Triage System (MTS) must have a high sensitivity to minimize the occurrence of under-triage, but must not compromise specificity to avoid the occurrence of overtriage. Sensitivity and specificity of the MTS can be calculated using the frequency of appropriately assigned clinical priority levels for patients presenting to the ED...
June 2017: JBI Database of Systematic Reviews and Implementation Reports
https://www.readbyqxmd.com/read/28625228/warnings-as-a-directive-front-of-pack-nutrition-labelling-scheme-comparison-with-the-guideline-daily-amount-and-traffic-light-systems
#10
Alejandra Arrúa, Leandro Machín, María Rosa Curutchet, Joseline Martínez, Lucía Antúnez, Florencia Alcaire, Ana Giménez, Gastón Ares
OBJECTIVE: Warnings have recently been proposed as a new type of directive front-of-pack (FOP) nutrition labelling scheme to flag products with high content of key nutrients. In the present work, this system was compared with the two most common FOP nutrition labelling schemes (Guideline Daily Amounts (GDA) and traffic-light system) in terms of goal-directed attention, influence on perceived healthfulness and ability to differentiate between products. Design/Setting/Subjects Goal-directed attention to FOP labels was evaluated using a visual search task in which participants were presented with labels on a computer screen and were asked to indicate whether labels with high sodium content were present or absent...
June 19, 2017: Public Health Nutrition
https://www.readbyqxmd.com/read/28623180/behavioral-screening-of-the-lopac-1280-library-in-zebrafish-embryos
#11
Sara M Vliet, Trina C Ho, David C Volz
Spontaneous activity represents an early, primitive form of motor activity within zebrafish embryos, providing a potential readout for identification of neuroactive compounds. However, despite use as an endpoint in chemical screens around the world, the predictive power and limitations of assays relying on spontaneous activity remain unclear. Using an improved high-content screening assay that increased throughput from 384 to 3072 wells per week, we screened a well-characterized library of 1280 pharmacologically active compounds (LOPAC(1280)) - 612 of which target neurotransmission - to identify which targets are detected using spontaneous activity as a readout...
June 13, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28622455/intestinal-metabolites-are-profoundly-altered-in-the-context-of-hla-b27-expression-and-functionally-modulate-disease-in-a-rat-model-of-spondyloarthropathy
#12
Mark Asquith, Sean Davin, Patrick Stauffer, Claire Michell, Cathleen Janowitz, Phoebe Lin, Joe Ensign-Lewis, Jason M Kinchen, Dennis R Koop, Jim Rosenbaum
Objective HLA-B27 associated spondyloarthropathies are associated with an altered intestinal microbiota and bowel inflammation. Therefore, we sought to identify B27-dependent changes in both host and microbial metabolites in the HLA-B27/β2m rat and whether microbiota-derived metabolites could impact disease in this major model of spondyloarthropathy. Methods Cecal contents were collected from 6wk (pre-diseased) and 16wk (diseased) Fischer 344 HLA-B27/β2m transgenic rats and WT controls. Metabolomic profiling was performed by high-throughput gas- and liquid-chromatography-based mass spectrometry...
June 16, 2017: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/28618188/real-time-viability-and-apoptosis-kinetic-detection-method-of-3d-multicellular-tumor-spheroids-using-the-celigo-image-cytometer
#13
Sarah Kessel, Scott Cribbes, Surekha Bonasu, William Rice, Jean Qiu, Leo Li-Ying Chan
The development of three-dimensional (3D) multicellular tumor spheroid models for cancer drug discovery research has increased in the recent years. The use of 3D tumor spheroid models may be more representative of the complex in vivo tumor microenvironments in comparison to two-dimensional (2D) assays. Currently, viability of 3D multicellular tumor spheroids has been commonly measured on standard plate-readers using metabolic reagents such as CellTiter-Glo® for end point analysis. Alternatively, high content image cytometers have been used to measure drug effects on spheroid size and viability...
June 15, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28616421/cystic-fibrosis-prevalence-among-a-group-of-high-risk-children-in-the-main-referral-children-hospital-in-iran
#14
Mohammad Reza Modaresi, Jamal Faghinia, Mohsen Reisi, Majid Keivanfar, Shiva Navaie, Javad Seyyedi, Faride Baharzade
BACKGROUND: Knowledge about cystic fibrosis (CF) in Iran is very limited. The objective of this study was to determine the prevalence of CF among a group of high-risk children with suggestive clinical features in the main referral hospital in Iran. MATERIALS AND METHODS: This study children consisted of 505 patients who had presented with one or more of the following symptoms: chronic or recurrent respiratory symptoms, gastrointestinal symptoms as rectal prolapse, steatorrhea, hepatobiliary disease as prolonged jaundice, failure to thrive, hyperglycemia and glycosuria, hypochloremic metabolic alkalosis, hypoprothrombinemia, anemia or edema, and positive family history of CF...
2017: Journal of Education and Health Promotion
https://www.readbyqxmd.com/read/28615943/the-use-of-functional-epirubicin-liposomes-to-induce-programmed-death-in-refractory-breast-cancer
#15
Lei Liu, Li-Min Mu, Yan Yan, Jia-Shuan Wu, Ying-Jie Hu, Ying-Zi Bu, Jing-Ying Zhang, Rui Liu, Xue-Qi Li, Wan-Liang Lu
Currently, chemotherapy is less efficient in controlling the continued development of breast cancer because it cannot eliminate extrinsic and intrinsic refractory cancers. In this study, mitochondria were modified by functional epirubicin liposomes to eliminate refractory cancers through initiation of an apoptosis cascade. The efficacy and mechanism of epirubicin liposomes were investigated on human breast cancer cells in vitro and in vivo using flow cytometry, confocal microscopy, high-content screening system, in vivo imaging system, and tumor inhibition in mice...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28612291/-f-18-av-1451-binding-correlates-with-postmortem-neurofibrillary-tangle-braak-staging
#16
Marta Marquié, Michael Siao Tick Chong, Alejandro Antón-Fernández, Eline E Verwer, Nil Sáez-Calveras, Avery C Meltzer, Prianca Ramanan, Ana C Amaral, Jose Gonzalez, Marc D Normandin, Matthew P Frosch, Teresa Gómez-Isla
[F-18]-AV-1451, a PET tracer specifically developed to detect brain neurofibrillary tau pathology, has the potential to facilitate accurate diagnosis of Alzheimer's disease (AD), staging of brain tau burden and monitoring disease progression. Recent PET studies show that patients with mild cognitive impairment and AD dementia exhibit significantly higher in vivo [F-18]-AV-1451 retention than cognitively normal controls. Importantly, PET patterns of [F-18]-AV-1451 correlate well with disease severity and seem to match the predicted topographic Braak staging of neurofibrillary tangles (NFTs) in AD, although this awaits confirmation...
June 13, 2017: Acta Neuropathologica
https://www.readbyqxmd.com/read/28611339/policy-of-screening-for-colorectal-cancer-in-saudi-arabia-a-prospective-analysis
#17
Abdulrahman A Aljumah, Abdulrahman M Aljebreen
Colorectal cancer (CRC) is one of the most common cancers worldwide. Several policies of CRC screening are available in different countries. The idea of screening is to identify patients at risk by detection of precancerous and small cancers early enough before they become advanced. In Saudi Arabia (SA), there is no countrywide policy for CRC screening despite the increasing incidence of the disease. Screening for CRC is a multidisciplinary approach that requires education programs, substantial financial support, several logistic measures, and predetermined resources before implementing such a program...
May 2017: Saudi Journal of Gastroenterology: Official Journal of the Saudi Gastroenterology Association
https://www.readbyqxmd.com/read/28609476/effective-strategies-to-motivate-nursing-home-residents-in-oral-care-and-to-prevent-or-reduce-responsive-behaviors-to-oral-care-a-systematic-review
#18
Matthias Hoben, Angelle Kent, Nadia Kobagi, Kha Tu Huynh, Alix Clarke, Minn N Yoon
BACKGROUND: Poor oral health has been a persistent problem in nursing home residents for decades, with severe consequences for residents and the health care system. Two major barriers to providing appropriate oral care are residents' responsive behaviors to oral care and residents' lack of ability or motivation to perform oral care on their own. OBJECTIVES: To evaluate the effectiveness of strategies that nursing home care providers can apply to either prevent/overcome residents' responsive behaviors to oral care, or enable/motivate residents to perform their own oral care...
2017: PloS One
https://www.readbyqxmd.com/read/28609451/anti-cancer-agents-in-saudi-arabian-herbals-revealed-by-automated-high-content-imaging
#19
Dina Hajjar, Stephan Kremb, Salim Sioud, Abdul-Hamid Emwas, Christian R Voolstra, Timothy Ravasi
Natural products have been used for medical applications since ancient times. Commonly, natural products are structurally complex chemical compounds that efficiently interact with their biological targets, making them useful drug candidates in cancer therapy. Here, we used cell-based phenotypic profiling and image-based high-content screening to study the mode of action and potential cellular targets of plants historically used in Saudi Arabia's traditional medicine. We compared the cytological profiles of fractions taken from Juniperus phoenicea (Arar), Anastatica hierochuntica (Kaff Maryam), and Citrullus colocynthis (Hanzal) with a set of reference compounds with established modes of action...
2017: PloS One
https://www.readbyqxmd.com/read/28608629/enantioselective-cytotoxicity-of-zns-mn-quantum-dots-in-a549-cells
#20
V A Kuznetsova, A K Visheratina, A Ryan, I V Martynenko, A Loudon, C M Maguire, F Purcell-Milton, A O Orlova, A V Baranov, A V Fedorov, A Prina-Mello, Y Volkov, Y K Gun'Ko
Chirality strongly influences many biological properties of materials, such as cell accumulation, enzymatic activity, and toxicity. In the past decade, it has been shown that quantum dots (QDs), fluorescent semiconductor nanoparticles with unique optical properties, can demonstrate optical activity due to chiral ligands bound on their surface. Optically active QDs could find potential applications in biomedical research, therapy, and diagnostics. Consequently, it is very important to investigate the interaction of QDs capped with chiral ligands with living cells...
June 13, 2017: Chirality
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