keyword
MENU ▼
Read by QxMD icon Read
search

High content screening

keyword
https://www.readbyqxmd.com/read/28437228/first-case-of-ndm-1-producing-klebsiella-pneumoniae-in-annaba-university-hospital-algeria
#1
Amel Abderrahim, Nassima Djahmi, Charlotte Pujol, Sabina Nedjai, Mohamed Cherif Bentakouk, Djamila Kirane-Gacemi, Mazouz Dekhil, Albert Sotto, Jean-Philippe Lavigne, Alix Pantel
AIM: The aim of this study was to characterize two carbapenem-resistant Klebsiella pneumoniae isolates recovered from urine samples in a patient hospitalized at Annaba University hospital (Algeria) in 2014. RESULTS: Two K. pneumoniae isolates were studied because they proved resistant to almost all antibiotics tested with a high level resistance to ertapenem (minimum inhibitory concentration = 32 mg/L). The results of modified Hodge test and combined disk test (ROSCO Diagnostica, Taastrup, Denmark) were positive...
February 22, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28437080/comparison-of-tumor-penetration-of-podophyllotoxin-carboxymethylcellulose-conjugates-with-various-chemical-compositions-in-tumor-spheroid-culture-and-in-vivo-solid-tumor
#2
Yang Yang, Aniruddha Roy, Yucheng Zhao, Elijus Undzys, Shyh-Dar Li
Polymer conjugation is an attractive approach for delivering insoluble and highly toxic drugs to tumors. However, most reports in the literature only disclose the optimal composition without emphasizing rational design or composition optimization to achieve maximized biological effects. In this study, we aimed to demonstrate that composition of a polymer conjugate would determine its physiochemical characteristics, tumor penetration and, ultimately, the in vivo efficacy. We also aimed to examine whether the tumor spheroid model could generate comparable results with the in vivo tumor model in terms of tumor penetration and efficacy of the various polymer conjugates...
April 24, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28434878/molecular-phenotyping-combines-molecular-information-biological-relevance-and-patient-data-to-improve-productivity-of-early-drug-discovery
#3
Faye Marie Drawnel, Jitao David Zhang, Erich Küng, Natsuyo Aoyama, Fethallah Benmansour, Andrea Araujo Del Rosario, Sannah Jensen Zoffmann, Frédéric Delobel, Michael Prummer, Franziska Weibel, Coby Carlson, Blake Anson, Roberto Iacone, Ulrich Certa, Thomas Singer, Martin Ebeling, Marco Prunotto
Today, novel therapeutics are identified in an environment which is intrinsically different from the clinical context in which they are ultimately evaluated. Using molecular phenotyping and an in vitro model of diabetic cardiomyopathy, we show that by quantifying pathway reporter gene expression, molecular phenotyping can cluster compounds based on pathway profiles and dissect associations between pathway activities and disease phenotypes simultaneously. Molecular phenotyping was applicable to compounds with a range of binding specificities and triaged false positives derived from high-content screening assays...
April 13, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28429185/high-content-assessment-of-cardiac-function-using-heart-on-a-chip-devices-as-drug-screening-model
#4
Genevieve Conant, Benjamin Fook Lun Lai, Rick Xing Ze Lu, Anastasia Korolj, Erika Yan Wang, Milica Radisic
Drug discovery and development continues to be a challenge to the pharmaceutical industry despite great advances in cell and molecular biology that allow for the design of better targeted therapeutics. Many potential drug compounds fail during the clinical trial due to inefficacy and toxicity that were not predicted during preclinical stages. The fundamental problem lies with the use of traditional drug screening models that still largely rely on the use of cell lines or animal cell monolayers, which leads to lack of predictive power of human tissue and organ response to the drug candidates...
April 20, 2017: Stem Cell Reviews
https://www.readbyqxmd.com/read/28423969/process-evaluation-of-the-type-2-diabetes-mellitus-pulse-program-randomized-controlled-trial-recruitment-engagement-and-overall-satisfaction
#5
Elroy J Aguiar, Philip J Morgan, Clare E Collins, Ronald C Plotnikoff, Myles D Young, Robin Callister
BACKGROUND: Men are underrepresented in weight loss and type 2 diabetes mellitus (T2DM) prevention studies. PURPOSE: To determine the effectiveness of recruitment, and acceptability of the T2DM Prevention Using LifeStyle Education (PULSE) Program-a gender-targeted, self-administered intervention for men. METHODS: Men (18-65 years, high risk for T2DM) were randomized to intervention ( n = 53) or wait-list control groups ( n = 48). The 6-month PULSE Program intervention focused on weight loss, diet, and exercise for T2DM prevention...
April 1, 2017: American Journal of Men's Health
https://www.readbyqxmd.com/read/28421600/lay-led-and-peer-support-interventions-for-adolescents-with-asthma
#6
REVIEW
Kayleigh M Kew, Robin Carr, Iain Crossingham
BACKGROUND: Adolescents with asthma are at high risk of poor adherence with treatment. This may be compounded by activities that worsen asthma, in particular smoking. Additional support above and beyond routine care has the potential to encourage good self-management. We wanted to find out whether sessions led by their peers or by lay leaders help to reduce these risks and improve asthma outcomes among adolescents. OBJECTIVES: To assess the safety and efficacy of lay-led and peer support interventions for adolescents with asthma...
April 19, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28413055/functional-genomics-and-high-content-screening-in-the-nervous-system
#7
EDITORIAL
Jessica K Lerch, William Buchser
No abstract text is available yet for this article.
April 2017: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/28409012/enhancing-the-diagnosis-of-fabry-disease-in-cardiology-with-a-targeted-information-a-before-after-control-impact-study
#8
Anne-Louise Savary, Remy Morello, Carole Brasse-Lagnel, Paul Milliez, Soumeya Bekri, Fabien Labombarda
BACKGROUND: Cardiac complications in Fabry disease are frequent and dominated by a high frequency of left ventricular hypertrophy; therefore, cardiologists may have an essential role in screening for this disease. Providing cardiologists with targeted information on Fabry disease would be valuable and could reduce both diagnostic and therapeutic delays. The aim of this study was to evaluate the efficiency of such strategy for Fabry screening. METHODS: We conducted a before-after control-impact study by comparing observations made before and after targeted information on Fabry disease among cardiologists...
2017: Open Heart
https://www.readbyqxmd.com/read/28405911/a-new-biopesticide-from-a-local-bacillus-thuringiensis-var-tenebrionis-xd3-against-alder-leaf-beetle-coleoptera-chrysomelidae
#9
Ardahan Eski, İsmail Demir, Kazım Sezen, Zihni Demirbağ
Use of chemical pesticides in agriculture harms humans, non-target organisms and environments, and causes increase resistance against chemicals. In order to develop an effective bio-pesticide against coleopterans, particularly against Agelastica alni (Coleoptera: Chrysomelidae) which is one of the serious pests of alder leaf and hazelnut, we tested the insecticidal effect of 21 Bacillus isolates against the larvae and adults of the pest. Bacillus thuringiensis var. tenebrionis-Xd3 (Btt-Xd3) showed the highest insecticidal effect based on screening tests...
May 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28403852/health-workers-perceptions-and-attitude-about-ghana-s-preparedness-towards-preventing-containing-and-managing-ebola-virus-disease
#10
Philip Baba Adongo, Philip Teg-Nefaah Tabong, Emmanuel Asampong, Joana Ansong, Magda Robalo, Richard M Adanu
BACKGROUND: Ebola virus is highly infectious and the disease can be very fatal. The World Health Organization has declared the 2014-2015 Ebola Virus Disease outbreak a Public Health Emergency of International Concern. In response to this, preparations were made in various health facilities and entry points across Ghana. This study explored health workers perceptions, and attitude about Ghana's preparedness towards preventing and containing Ebola Virus Disease. METHODS: We conducted a qualitative study in five (5) of the ten (10) regions in Ghana...
April 12, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/28402311/polishing-of-municipal-secondary-effluent-using-native-microalgae-consortia
#11
Julio César Beltrán-Rocha, Icela Dagmar Barceló-Quintal, Magdalena García-Martínez, Luis Osornio-Berthet, Nidia Saavedra-Villarreal, Juan Villarreal-Chiu, Ulrico Javier López-Chuken
This work evaluates the use of native microalgae consortia for a dual role: polishing treatment of municipal wastewater effluents and microalgae biomass feedstock potential for biodiesel or biofertilizer production. An initial screening was undertaken to test N and P removal from secondary effluents and biomass production by 12 consortia. A subsequent treatment was performed by selected consortia (01 and 12) under three operational conditions: stirring (S), S + 12 h of daily aeration (S + A) and S + A enriched with CO2 (S + AC)...
April 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28402035/screening-and-investigation-lactobacillius-spp-to-improve-secale-cereale-silage-quality
#12
Hye Sun Kim, Ouk Kyu Han, Sam Churl Kim, Min Jung Kim, Youn-Sig Kwak
Silage is a high-moisture-content forage that is used to feed livestock. Using silage as feed is economically feasible and suitable for cattle management. Secale cereale is considered as a high-quality forage plant. After the heading stage the biomass of S. cereale increases by up to 30%; however, lignification in the cell wall causes low fermentation efficiency and coefficient digestibility, and it may also be contaminated by mycotoxin-producing fungi and can impair the quality of the silage. Therefore, the aim of this study was to screen beneficial Lactobacillius spp...
April 12, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28401619/non-compliance-to-infectious-disease-deferral-criteria-among-hong-kong-s-blood-donors
#13
S S Lee, E K H Cheung, J N S Leung, C K Lee
BACKGROUND AND OBJECTIVES: Donor screening alone cannot eliminate the risk of transfusion-transmitted HIV infection. Donor deferral according to established criteria is a supplemental strategy, which has focused largely on men who have sex with men (MSM). A study was conducted to determine the compliance of non-MSM donors with such criteria and examine its implications on blood safety. MATERIALS AND METHODS: Chinese donors who have just donated blood at blood donor centres in Hong Kong were recruited...
April 11, 2017: Vox Sanguinis
https://www.readbyqxmd.com/read/28401382/first-application-of-newly-developed-ft-nir-spectroscopic-methodology-to-predict-authenticity-of-extra-virgin-olive-oil-retail-products-in-the-usa
#14
Magdi M Mossoba, Hormoz Azizian, Ali Reza Fardin-Kia, Sanjeewa R Karunathilaka, John K G Kramer
Economically motivated adulteration (EMA) of extra virgin olive oils (EVOO) has been a worldwide problem and a concern for government regulators for a long time. The US Food and Drug Administration (FDA) is mandated to protect the US public against intentional adulteration of foods and has jurisdiction over deceptive label declarations. To detect EMA of olive oil and address food safety vulnerabilities, we used a previously developed rapid screening methodology to authenticate EVOO. For the first time, a recently developed FT-NIR spectroscopic methodology in conjunction with partial least squares analysis was applied to commercial products labeled EVOO purchased in College Park, MD, USA to rapidly predict whether they are authentic, potentially mixed with refined olive oil (RO) or other vegetable oil(s), or are of lower quality...
April 11, 2017: Lipids
https://www.readbyqxmd.com/read/28397941/cytotoxicity-anticancer-activities-of-anastrozole-against-breast-liver-hepatocellular-and-prostate-cancer-cells
#15
Firas Hassan, Gamal A El-Hiti, Mays Abd-Allateef, Emad Yousif
To investigate the cytotoxic effect of anastrozole on breast (MCF7), liver hepatocellular (HepG2), and prostate (PC3) cancer cells. Methods: This is a prospective study. Anastrozole's mechanism of apoptosis in living cells was also determined by high content screening (HCS) assay. Methylthiazol tetrazolium (MTT) assay was carried out at the Centre of Biotechnology Research's, Al-Nahrain University, Baghdad, Iraq between July 2015 and October 2015. The HCS assay was performed at the Centre for Natural Product Research  and Drug Discovery, Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia between November 2015 and February 2016...
April 2017: Saudi Medical Journal
https://www.readbyqxmd.com/read/28394930/analysis-of-host-microrna-function-uncovers-a-role-for-mir-29b-2-5p-in-shigella-capture-by-filopodia
#16
Ushasree Sunkavalli, Carmen Aguilar, Ricardo Jorge Silva, Malvika Sharan, Ana Rita Cruz, Caroline Tawk, Claire Maudet, Miguel Mano, Ana Eulalio
MicroRNAs play an important role in the interplay between bacterial pathogens and host cells, participating as host defense mechanisms, as well as exploited by bacteria to subvert host cellular functions. Here, we show that microRNAs modulate infection by Shigella flexneri, a major causative agent of bacillary dysentery in humans. Specifically, we characterize the dual regulatory role of miR-29b-2-5p during infection, showing that this microRNA strongly favors Shigella infection by promoting both bacterial binding to host cells and intracellular replication...
April 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28391735/diet-derived-changes-by-sourdough-fermented-rye-bread-in-exhaled-breath-aspiration-ion-mobility-spectrometry-profiles-in-individuals-with-mild-gastrointestinal-symptoms
#17
Kaisa Raninen, Jenni Lappi, Mikko Kolehmainen, Marjukka Kolehmainen, Hannu Mykkänen, Kaisa Poutanen, Olavi Raatikainen
The potential of utilising exhaled breath volatile organic compound (VOC) profiles in studying diet-derived metabolic changes was examined. After a four-week initial diet period with white wheat bread (WW), seven participants received in randomised order high-fibre diets containing sourdough whole grain rye bread (WGR) or white wheat bread enriched with bioprocessed rye bran (WW + BRB), both for 4 weeks. Alveolar exhaled breath samples were analysed with ChemPro(®)100i analyser (Environics OY, Mikkeli, Finland) at the end of each diet period in fasting state and after a standardised meal...
April 9, 2017: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/28387709/screening-for-cd-safe-cultivars-of-chinese-cabbage-and-a-preliminary-study-on-the-mechanisms-of-cd-accumulation
#18
Jingjie Wang, Nan Yu, Guangmao Mu, Kamran I Shinwari, Zhenguo Shen, Luqing Zheng
With the rapid progress of industrialization, the effects of environmental contamination on plant toxicity, and subsequently on human health, is a growing concern. For example, the heavy metal pollution of soil such as that caused by cadmium (Cd) is a serious threat. Therefore, screening for pollution-safe edible plants is an essential approach for growing plants under heavy metal-contaminated soils. In the current study, 35 Chinese cabbage (Brassica pekinensis L.) cultivars were selected with the aim of screening for Cd-safe cultivars (CSCs), analyzing their safety, and exploring the mechanism of Cd accumulation...
April 7, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28381964/biological-control-of-bacterial-fruit-blotch-of-watermelon-pathogen-acidovorax-citrulli-with-rhizosphere-associated-bacteria
#19
Mahesh Adhikari, Dil Raj Yadav, Sang Woo Kim, Young Hyun Um, Hyun Seung Kim, Seong Chan Lee, Jeong Young Song, Hong Gi Kim, Youn Su Lee
Bacterial fruit blotch (BFB), which is caused by Acidovorax citrulli, is a serious threat to watermelon growers around the world. The present study was conducted to screen effective rhizobacterial isolates against 35 different A. citrulli isolates and determine their efficacy on BFB and growth parameters of watermelon. Two rhizobacterial isolates viz. Paenibacillus polymyxa (SN-22), Sinomonas atrocyanea (NSB-27) showed high inhibitory activity in the preliminary screening and were further evaluated for their effect on BFB and growth parameters of three different watermelon varieties under greenhouse conditions...
April 2017: Plant Pathology Journal
https://www.readbyqxmd.com/read/28381523/human-milk-oligosaccharide-composition-predicts-risk-of-necrotising-enterocolitis-in-preterm-infants
#20
Chloe A Autran, Benjamin P Kellman, Jae H Kim, Elizabeth Asztalos, Arlin B Blood, Erin C Hamilton Spence, Aloka L Patel, Jiayi Hou, Nathan E Lewis, Lars Bode
OBJECTIVE: Necrotising enterocolitis (NEC) is one of the most common and often fatal intestinal disorders in preterm infants. Markers to identify at-risk infants as well as therapies to prevent and treat NEC are limited and urgently needed. NEC incidence is significantly lower in breast-fed compared with formula-fed infants. Infant formula lacks human milk oligosaccharides (HMO), such as disialyllacto-N-tetraose (DSLNT), which prevents NEC in neonatal rats. However, it is unknown if DSLNT also protects human preterm infants...
April 5, 2017: Gut
keyword
keyword
16674
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"