keyword
MENU ▼
Read by QxMD icon Read
search

High content screening

keyword
https://www.readbyqxmd.com/read/29793009/evidence-on-the-measurement-properties-of-health-related-quality-of-life-instruments-is-largely-missing-in-patients-with-low-back-pain-a-systematic-review
#1
REVIEW
Alessandro Chiarotto, Caroline B Terwee, Steven J Kamper, Maarten Boers, Raymond W Ostelo
OBJECTIVE: To synthesize the measurement properties of five health-related quality of life (HRQoL) instruments [Short Form 36 (SF-36), Short Form 12 (SF-12), EuroQol 5D (EQ-5D), Nottingham Health Profile (NHP), PROMIS Global Health (PROMIS-GH-10)] in patients with low back pain (LBP). STUDY DESIGN AND SETTING: Six electronic databases (MEDLINE, EMBASE, CINAHL, PsycINFO, SportDiscus, Google Scholar) were searched (July 2017). Studies assessing any measurement property in non-specific LBP patients were included...
May 21, 2018: Journal of Clinical Epidemiology
https://www.readbyqxmd.com/read/29787963/sirt1-activator-e1231-protects-from-experimental-atherosclerosis-and-lowers-plasma-cholesterol-and-triglycerides-by-enhancing-abca1-expression
#2
Tingting Feng, Peng Liu, Xiao Wang, Jinque Luo, Xuan Zuo, Xinhai Jiang, Chang Liu, Yongzhen Li, Ni Li, Minghua Chen, Ningyu Zhu, Xiaowan Han, Chao Liu, Yanni Xu, Shuyi Si
BACKGROUND AND AIMS: Sirtuin 1 (SIRT1) is a nicotinamide adenine dinucleotide-dependent protein deacetylase. Recent studies have demonstrated that enhancing SIRT1 expression or activity may modulate cholesterol and lipid metabolism. However, pharmacological and molecular regulators for SIRT1 are scarce. Here, we aimed to find novel small molecule modulators of SIRT1 to regulate cholesterol and lipid metabolism. METHODS: A high-throughput screening assay was established to identify SIRT1 activators...
April 30, 2018: Atherosclerosis
https://www.readbyqxmd.com/read/29779890/high-throughput-screening-enhances-kidney-organoid-differentiation-from-human-pluripotent-stem-cells-and-enables-automated-multidimensional-phenotyping
#3
Stefan M Czerniecki, Nelly M Cruz, Jennifer L Harder, Rajasree Menon, James Annis, Edgar A Otto, Ramila E Gulieva, Laura V Islas, Yong Kyun Kim, Linh M Tran, Timothy J Martins, Jeffrey W Pippin, Hongxia Fu, Matthias Kretzler, Stuart J Shankland, Jonathan Himmelfarb, Randall T Moon, Neal Paragas, Benjamin S Freedman
Organoids derived from human pluripotent stem cells are a potentially powerful tool for high-throughput screening (HTS), but the complexity of organoid cultures poses a significant challenge for miniaturization and automation. Here, we present a fully automated, HTS-compatible platform for enhanced differentiation and phenotyping of human kidney organoids. The entire 21-day protocol, from plating to differentiation to analysis, can be performed automatically by liquid-handling robots, or alternatively by manual pipetting...
May 15, 2018: Cell Stem Cell
https://www.readbyqxmd.com/read/29779429/genotoxicity-evaluation-of-carbon-monoxide-and-1-3-butadiene-using-a-new-joint-technology-the-in-vitro-%C3%AE-h2ax-hcs-assay-combined-with-air-liquid-interface-system
#4
Sen Zhang, Huan Chen, An Wang, Yong Liu, Hongwei Hou, Qingyuan Hu
To investigate the genotoxicity of gaseous toxicants CO and 1,3-butadiene in vitro, a novel combination technology-the in vitro γH2AX high content screening assay combined with air-liquid interface system was established. The results showed that this new technology was available and effective. Based on the joint technology, genotoxicity of CO and 1,3-butadiene was evaluated further in this study. The results showed that treatment concentrations (0, 20%,40%, 80% and 100%, v/v) and exposure time (15, 30, 45, 60 and 90 min) of CO both had no statistically significant effects on the induction of γH2AX (p > 0...
May 21, 2018: Toxicology Mechanisms and Methods
https://www.readbyqxmd.com/read/29777802/derivation-and-characterization-of-a-ucp1-reporter-human-es-cell-line
#5
Suranjit Mukherjee, Tuo Zhang, Lauretta A Lacko, Lei Tan, Jenny Zhaoying Xiang, Jason M Butler, Shuibing Chen
Interest in human brown fat as a novel therapeutic target to tackle the growing obesity and diabetes epidemic has increased dramatically in recent years. While much insight into brown fat biology has been gained from murine cell lines and models, few resources are available to study human brown fat in vitro, which makes the need for new ways to derive and study human brown adipocytes imperative. Human ES cell based reporter systems present an excellent tool to identify, mark, and purify cell populations of choice...
April 22, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29777241/acute-effect-of-equicaloric-meals-varying-in-glycemic-index-and-glycemic-load-on-arterial-stiffness-and-glycemia-in-healthy-adults-a-randomized-crossover-trial
#6
Andreea Zurbau, Alexandra L Jenkins, Elena Jovanovski, Fei Au-Yeung, Emma A Bateman, Christy Brissette, Thomas M S Wolever, Amir Hanna, Vladimir Vuksan
BACKGROUND/OBJECTIVES: Dietary carbohydrate quality and quantity fluctuate but it is unknown which attribute takes precedence in vascular health preservation. We investigated all four permutations of glycemic index (GI) and glycemic load (GL) on acute vascular and glycemic responses. SUBJECTS/METHODS: Twenty-one healthy adults were screened for this crossover trial. Seventeen (8 M:9 F; 26.7 ± 12.3 y; BMI 22.2 ± 2.8 kg/m2 ) entered randomization and completed the study, receiving four isocaloric meals, varying in GI and GL, in random order at least 3 days apart...
May 17, 2018: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/29776356/policy-development-and-challenges-of-global-mental-health-a-systematic-review-of-published-studies-of-national-level-mental-health-policies
#7
Wei Zhou, Yu Yu, Mei Yang, Lizhang Chen, Shuiyuan Xiao
BACKGROUND: Mental health policy can be an essential and powerful tool to improve a population's mental health. However, around one third of countries do not possess a mental health policy, and there are large disparities in population coverage rates between high- and low-income countries. The goal of this study is to identify the transition and implementation challenges of mental health policies in both high-income countries (HICs) as well as middle- and low-income countries (MLICs)...
May 18, 2018: BMC Psychiatry
https://www.readbyqxmd.com/read/29769289/metarrestin-a-perinucleolar-compartment-inhibitor-effectively-suppresses-metastasis
#8
Kevin J Frankowski, Chen Wang, Samarjit Patnaik, Frank J Schoenen, Noel Southall, Dandan Li, Yaroslav Teper, Wei Sun, Irawati Kandela, Deqing Hu, Christopher Dextras, Zachary Knotts, Yansong Bian, John Norton, Steve Titus, Marzena A Lewandowska, Yiping Wen, Katherine I Farley, Lesley Mathews Griner, Jamey Sultan, Zhaojing Meng, Ming Zhou, Tomas Vilimas, Astin S Powers, Serguei Kozlov, Kunio Nagashima, Humair S Quadri, Min Fang, Charles Long, Ojus Khanolkar, Warren Chen, Jinsol Kang, Helen Huang, Eric Chow, Esthermanya Goldberg, Coral Feldman, Romi Xi, Hye Rim Kim, Gary Sahagian, Susan J Baserga, Andrew Mazar, Marc Ferrer, Wei Zheng, Ali Shilatifard, Jeffrey Aubé, Udo Rudloff, Juan Jose Marugan, Sui Huang
Metastasis remains a leading cause of cancer mortality due to the lack of specific inhibitors against this complex process. To identify compounds selectively targeting the metastatic state, we used the perinuclear compartment (PNC), a complex nuclear structure associated with metastatic behaviors of cancer cells, as a phenotypic marker for a high-content screen of over 140,000 structurally diverse compounds. Metarrestin, obtained through optimization of a screening hit, disassembles PNCs in multiple cancer cell lines, inhibits invasion in vitro, suppresses metastatic development in three mouse models of human cancer, and extends survival of mice in a metastatic pancreatic cancer xenograft model with no organ toxicity or discernable adverse effects...
May 16, 2018: Science Translational Medicine
https://www.readbyqxmd.com/read/29768981/a-scalable-pipeline-for-high-throughput-flow-cytometry
#9
Aaron C Wilson, Ioannis K Moutsatsos, Gary Yu, Javier J Pineda, Yan Feng, Douglas S Auld
Flow cytometry (FC) provides high-content data for a variety of applications, including phenotypic analysis of cell surface and intracellular markers, characterization of cell supernatant or lysates, and gene expression analysis. Historically, sample preparation, acquisition, and analysis have presented as a bottleneck for running such types of assays at scale. This article will outline the solutions that have been implemented at Novartis which have allowed high-throughput FC to be successfully conducted and analyzed for a variety of cell-based assays...
May 1, 2018: SLAS Discovery
https://www.readbyqxmd.com/read/29768479/construction-of-a-tri-chromatic-reporter-cell-line-for-the-rapid-and-simple-screening-of-splice-switching-oligonucleotides-targeting-dmd-exon-51-using-high-content-screening
#10
Takenori Shimo, Keisuke Tachibana, Satoshi Obika
Splice-switching oligonucleotides (SSOs) that can modulate RNA splicing are used for the treatment of many genetic disorders. To enhance the efficacy of modulating splicing, it is important to optimize SSOs with regard to target sites, GC content, melting temperature (Tm value), chemistries, and lengths. Thus, in vitro assay systems that allow for the rapid and simple screening of SSOs are essential for optimizing SSO design. In this study, we established a novel tri-chromatic reporter cell line for SSO screening...
2018: PloS One
https://www.readbyqxmd.com/read/29768359/case-report-of-gastric-distension-due-to-superior-mesenteric-artery-syndrome-mimicking-hollow-viscus-perforation-considerations-in-critical-care-ultrasound
#11
Yan-Mei Feng, Dong Wan, Rui Guo
RATIONALE: Critical care ultrasound identifies the signs of free intraperitoneal air and echogenic free fluid always indicates hollow viscus perforation (HVP) and needs immediate surgical interventions. However, in rare cases, these classic signs may also mislead proper clinical decisions. We report perforated viscus associated large peritoneal effusion with initial critical care ultrasound findings, whereas computed tomography (CT) examination confirmed a giant stomach due to superior mesenteric artery syndrome (SMAS)...
May 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29765202/inhibition-of-human-prostate-cancer-pc-3-cells-and-targeting-of-pc-3-derived-prostate-cancer-stem-cells-with-koenimbin-a-natural-dietary-compound-from-murraya-koenigii-l-spreng
#12
Behnam Kamalidehghan, Soudeh Ghafouri-Fard, Elahe Motevaseli, Fatemeh Ahmadipour
Background: Inhibition of prostate cancer stem cells (PCSCs) is an efficient curative maintenance protocol for the prevention of prostate cancer. The objectives of this study were to assess the efficiency of koenimbin, a major biologically active component of Murraya koenigii (L) Spreng, in the suppression of PC-3 cells and to target PC-3-derived cancer stem cells (CSCs) through apoptotic and CSC signaling pathways in vitro. Materials and methods: The antiproliferative activity of koenimbin was examined using MTT, and the apoptotic detection was carried out by acridine orange/propidium iodide (AO/PI) double-staining and multiparametric high-content screening (HCS) assays...
2018: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/29763427/correction-a-high-content-assay-enables-the-automated-screening-and-identification-of-small-molecules-with-specific-aldh1a1-inhibitory-activity
#13
Adam Yasgar, Steven A Titus, Yuhong Wang, Carina Danchik, Shyh-Ming Yang, Vasilis Vasiliou, Ajit Jadhav, David J Maloney, Anton Simeonov, Natalia J Martinez
[This corrects the article DOI: 10.1371/journal.pone.0170937.].
2018: PloS One
https://www.readbyqxmd.com/read/29762554/a-multi-phenotypic-imaging-screen-to-identify-bacterial-effectors-by-exogenous-expression-in-a-hela-cell-line
#14
Adam Collins, Alan Huett
We present a high-content screen (HCS) for the simultaneous analysis of multiple phenotypes in HeLa cells expressing an autophagy reporter (mcherry-LC3) and one of 224 GFP-fused proteins from the Crohn's Disease (CD)-associated bacterium, Adherent Invasive E. coli (AIEC) strain LF82. Using automated confocal microscopy and image analysis (CellProfiler), we localised GFP fusions within cells, and monitored their effects upon autophagy (an important innate cellular defence mechanism), cellular and nuclear morphology, and the actin cytoskeleton...
May 15, 2018: Scientific Data
https://www.readbyqxmd.com/read/29755350/extracts-from-hypericum-hircinum-subsp-majus-exert-antifungal-activity-against-a-panel-of-sensitive-and-drug-resistant-clinical-strains
#15
Noemi Tocci, Daniele Perenzoni, Duilio Iamonico, Francesca Fava, Tobias Weil, Fulvio Mattivi
During the last two decades incidences of fungal infections dramatically increased and the often accompanying failure of available antifungal therapies represents a substantial clinical problem. The urgent need for novel antimycotics called particular attention to the study of natural products. The genus Hypericum includes many species that are used in the traditional medicine to treat pathological states like inflammations and infections caused by fungi. However, despite the diffused use of Hypericum -based products the antifungal potential of the genus is still poorly investigated...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29752102/cyclodextrin-and-dendrimer-conjugated-graphene-oxide-as-a-nanocarrier-for-the-delivery-of-selected-chemotherapeutic-and-photosensitizing-agents
#16
Ampornphan Siriviriyanun, Ya-Ju Tsai, Siew Hui Voon, Siaw Fui Kiew, Toyoko Imae, Lik Voon Kiew, Chung Yeng Looi, Won Fen Wong, Hong Boon Lee, Lip Yong Chung
In this study, nanohybrid materials consisting of graphene oxide (GO), β‑cyclodextrin (CD) and poly(amido amine) dendrimer (DEN) were successfully prepared by covalent bonding. GO-CD and GO-CD-DEN were found to be potential nanocarriers for anticancer drugs including chemotherapeutics (doxorubicin (DOX), camptothecin (CPT)) and photosensitizer (protoporphyrin IX (PpIX)). GO-CD possessed 1.2 times higher DOX-loading capacity than GO due to inclusion of additional DOX to the CD. The drug loading on GO-CD-DEN increased in the order: DOX < PpIX < CPT...
August 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29751846/generation-of-three-dimensional-human-neuronal-cultures-application-to-modeling-cns-viral-infections
#17
Leonardo D'Aiuto, Jennifer Naciri, Nicholas Radio, Sesha Tekur, Dennis Clayton, Gerard Apodaca, Roberto Di Maio, Yun Zhi, Peter Dimitrion, Paolo Piazza, Matthew Demers, Joel Wood, Charleen Chu, Jason Callio, Lora McClain, Robert Yolken, James McNulty, Paul Kinchington, David Bloom, Vishwajit Nimgaonkar
BACKGROUND: A variety of neurological disorders including neurodegenerative diseases and infection by neurotropic viruses can cause structural and functional changes in the central nervous system (CNS), resulting in long-term neurological sequelae. An improved understanding of the pathogenesis of these disorders is important for developing efficacious interventions. Human induced pluripotent stem cells (hiPSCs) offer an extraordinary window for modeling pathogen-CNS interactions, and other cellular interactions, in three-dimensional (3D) neuronal cultures that can recapitulate several aspects of in vivo brain tissue...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29751251/salt-tolerance-of-glycine-max-l-induced-by-endophytic-fungus-aspergillus-flavus-csh1-via-regulating-its-endogenous-hormones-and-antioxidative-system
#18
Lubna, Sajjad Asaf, Muhammad Hamayun, Abdul Latif Khan, Muhammad Waqas, Muhammad Aaqil Khan, Rahmatullah Jan, In-Jung Lee, Anwar Hussain
Abiotic stress resistance strategies are powerful approaches to sustainable agriculture because they reduce chemical input and enhance plant productivity. In current study, an endophytic fungus, Aspergillus flavus CHS1 was isolated from Chenopodium album Roots. CHS1 was initially screened for growth promoting activities like siderphore, phosphate solubilization, and the production of indole acetic acid and gibberellins and were further assayed for its ability to promote the growth of mutant Waito-C rice. The results revealed that different plant growth characteristic such as chlorophyll content, root-shoot length, and biomass production were significantly promoted during CHS1 treatment...
May 4, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29748799/metal-contamination-in-quail-meat-residues-sources-molecular-biomarkers-and-human-health-risk-assessment
#19
Wageh Sobhy Darwish, Amira Samir Atia, Mariam H E Khedr, Walaa Fathy Saad Eldin
Quail meat is an emerging source of high-quality animal protein. Quails are exposed to a wide range of xenobiotics such as heavy metals. In this study, residual concentrations of four toxic metals, of significant public health importance, including cadmium (Cd), lead (Pb), arsenic (As), and nickel (Ni), were determined in edible tissues of quails. In addition, metal loads were measured in water, feed, and litter samples collected from same quail farms as possible sources for quail exposure to heavy metals. The possible use of metallothionein (MT) and heat shock protein 70 (Hsp70) as molecular biomarkers of exposure to heavy metals was further investigated...
May 10, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29744906/on-line-high-performance-liquid-chromatography-coupled-with-biochemical-detection-method-for-screening-of-%C3%AE-glucosidase-inhibitors-in-green-tea
#20
Peng-Cheng Guo, Hua-Dan Shen, Jiang-Ji Fang, Tian-Ming Ding, Xiao-Ping Ding, Jun-Feng Liu
An on-line high performance liquid chromatography- biochemical detection (HPLC-BCD) method, which compounds separated by HPLC were on-line reacted with enzyme and substrate solutions delivered by flow injection and enzyme inhibition signal was collected in time by UV detector, was developed to rapidly screen α-glucosidase inhibitors from green tea extracts in this study. The chromatographic fingerprints and enzyme inhibition profiles of the different brands of green tea could be simultaneously detected by the on-line HPLC-BCD method...
May 9, 2018: Biomedical Chromatography: BMC
keyword
keyword
16674
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"