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High content screening

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https://www.readbyqxmd.com/read/28736217/high-content-screening-in-hpsc-neural-progenitors-identifies-drug-candidates-that-inhibit-zika-virus-infection-in-fetal-like-organoids-and-adult-brain
#1
Ting Zhou, Lei Tan, Gustav Y Cederquist, Yujie Fan, Brigham J Hartley, Suranjit Mukherjee, Mark Tomishima, Kristen J Brennand, Qisheng Zhang, Robert E Schwartz, Todd Evans, Lorenz Studer, Shuibing Chen
Zika virus (ZIKV) infects fetal and adult human brain and is associated with serious neurological complications. To date, no therapeutic treatment is available to treat ZIKV-infected patients. We performed a high-content chemical screen using human pluripotent stem cell-derived cortical neural progenitor cells (hNPCs) and found that hippeastrine hydrobromide (HH) and amodiaquine dihydrochloride dihydrate (AQ) can inhibit ZIKV infection in hNPCs. Further validation showed that HH also rescues ZIKV-induced growth and differentiation defects in hNPCs and human fetal-like forebrain organoids...
July 19, 2017: Cell Stem Cell
https://www.readbyqxmd.com/read/28735580/high-protein-goat-s-milk-diet-identified-through-newborn-screening-clinical-warning-of-a-potentially-dangerous-dietetic-practice
#2
Evelina Maines, Giorgia Gugelmo, Elisa Tadiotto, Angelo Pietrobelli, Natascia Campostrini, Andrea Pasini, Florina Ion-Popa, Monica Vincenzi, Francesca Teofoli, Marta Camilot, Andrea Bordugo
OBJECTIVE: Breast-feeding is an unequalled way of providing optimal food for infants' healthy growth and development and the WHO recommends that infants should be exclusively breast-fed for the first 6 months of life. For mothers who are unable to breast-feed or who decide not to, infant formulas are the safest alternative. Despite recommendations, it is possible that parents make potentially harmful nutritional choices for their children because of cultural beliefs or misinformation on infant nutrition...
July 24, 2017: Public Health Nutrition
https://www.readbyqxmd.com/read/28734895/a-sensitive-and-convenient-method-for-clinical-detection-of-non-syndromic-hearing-loss-associated-common-mutations
#3
Er-Feng Yuan, Wei Xia, Jing-Tao Huang, Ling Hu, Xing Liao, Xiang Dai, Song-Mei Liu
BACKGROUND: The majority of non-syndromic hearing loss (NSHL) patients result from causative mutations in GJB2, SLC26A4 and mitochondrial 12S rRNA genes. Accurate detection of these genetic mutations is increasingly recognized for its clinical significance to reduce incidence and guide individual treatment of NSHL. Current methods for clinical practice are labor intensive, expensive or of low sensitivity. METHODS: Genomic DNA from 7 newborns not passing the hearing screening and 94 newborns passing the hearing screening were analyzed for the common mutations using high resolution melting analysis (HRMA) and Sanger sequencing...
July 19, 2017: Gene
https://www.readbyqxmd.com/read/28733937/sequential-extraction-as-novel-approach-to-compare-12-medicinal-plants-from-kenya-regarding-their-potential-to-release-chromium-manganese-copper-and-zinc
#4
R Mogwasi, S Zor, D K Kariuki, M Z Getenga, V Nischwitz
This study is focusing on a novel approach to screen a large number of medicinal plants from Kenya regarding their contents and availability of selected metals potentially relevant for treatment of diabetes patients. For this purpose, total levels of zinc, chromium, manganese, and copper were determined by flame atomic absorption spectrometry and inductively coupled plasma mass spectrometry as well as BCR sequential extraction to fractionate the elemental species in anti-diabetic medicinal plants collected from five natural locations in two sub counties in Nyamira County, Kenya...
July 21, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28732697/cytological-study-of-dna-content-and-nuclear-morphometric-analysis-for-aid-in-the-diagnosis-of-high-grade-dysplasia-within-oral-leukoplakia
#5
Xi Yang, Xuan Xiao, Wenyan Wu, Xuemin Shen, Zengtong Zhou, Wei Liu, Linjun Shi
OBJECTIVE: To quantitatively examine the DNA content and nuclear morphometric status of oral leukoplakia (OL) and investigate its association with the degree of dysplasia in a cytologic study. STUDY DESIGN: Oral cytobrush biopsy was carried out to obtain exfoliative epithelial cells from lesions before scalpel biopsy at the same location in a blinded series of 70 patients with OL. Analysis of nuclear morphometry and DNA content status using image cytometry was performed with oral smears stained with the Feulgen-thionin method...
July 18, 2017: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
https://www.readbyqxmd.com/read/28732339/aryl-and-alkyl-phosphorus-containing-flame-retardants-induced-mitochondrial-impairment-and-cell-death-in-chinese-hamster-ovary-cho-k1-cells
#6
Chao Huang, Na Li, Shengwu Yuan, Xiaoya Ji, Mei Ma, Kaifeng Rao, Zijian Wang
Phosphorus-containing flame retardants (PFRs) are increasingly in demand worldwide as replacements for brominated flame retardants (BFRs), but insufficient available toxicological information on PFRs makes assessing their health risks challenging. Mitochondria are important targets of various environmental pollutants, and mitochondrial dysfunction may lead to many common diseases. In the present study, mitochondria impairment-related endpoints were measured by a high content screening (HCS) assay for 11 selected non-halogen PFRs in Chinese hamster ovary (CHO-k1) cells...
July 18, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28731783/identification-of-sppl2a-inhibitors-by-multiparametric-analysis-of-a-high-content-ultra-high-throughput-screen
#7
Xian Zhang, Marjo Götte, Yvonne Ibig-Rehm, Ansgar Schuffenhauer, Marion Kamke, Dan Beisner, Danilo Guerini, Daniela Siebert, Ghislain M C Bonamy, Daniela Gabriel, Ursula Bodendorf
The intramembrane protease signal peptide peptidase-like 2a (SPPL2a) is a potential drug target for the treatment of autoimmune diseases due to an essential role in B cells and dendritic cells. To screen a library of 1.4 million compounds for inhibitors of SPPL2a, we developed an imaging assay detecting nuclear translocation of the proteolytically released cytosolic substrate fragment. The state-of-the-art hit calling approach based on nuclear translocation resulted in numerous false-positive hits, mainly interrupting intracellular protein trafficking...
July 1, 2017: SLAS Discovery
https://www.readbyqxmd.com/read/28730478/high-content-imaging-and-rnai-screens-for-investigating-kinase-network-plasticity
#8
Simon R Stockwell, Sibylle Mittnacht
High-content imaging connects the information-rich method of microscopy with the systematic objective principles of software-driven analysis. Suited to automation and, therefore, considerable scale-up of study size, this approach can deliver multiparametric data over cell populations or at the level of the individual cell and has found considerable utility in reverse genetic and pharmacological screens. Here we present a method to screen small interfering RNA (siRNA) libraries allowing subsequent observation of the impact of each knockdown on two interlinked, high-content, G1-/S-phase cell cycle transition assays related to cyclin-dependent kinase (CDK) 2 activity...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28729835/uplc-esi-ms-ms-and-hptlc-method-for-quantitative-estimation-of-cytotoxic-glycosides-and-aglycone-in-bioactivity-guided-fractions-of-solanum-nigrum-l
#9
Karishma Chester, Sarvesh Paliwal, Washim Khan, Sayeed Ahmad
Solanum nigrum L., is traditionally used for the management of the various liver disorders. Investigating the effect of polarity based fractionation of S. nigrum for its hepatoprotective effect on Hep G2 cells in vitro to provide base of its activity by quantifying in steroidal glycosides responsible for hepatoprotective potential. A new UPLC-ESI-MS/MS method following a high performance thin layer chromatography (HPTLC) has been developed and validated for quantification of steroidal glycosides and aglycone (solasonine, solamargine, and solasodine, respectively)...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28727521/ethical-guidance-for-disaster-response-specifically-around-crisis-standards-of-care-a-systematic-review
#10
Jonathon P Leider, Debra DeBruin, Nicole Reynolds, Angelica Koch, Judy Seaberg
BACKGROUND: Terrorism, disease outbreaks, and other natural disasters and mass casualty events have pushed health care and public health systems to identify and refine emergency preparedness protocols for disaster response. Ethical guidance, alongside legal and medical frameworks, are increasingly common components of disaster response plans. OBJECTIVES: To systematically review the prevalence and content of ethical guidance offered for disaster response, specifically around crisis standards of care (CSCs)...
July 20, 2017: American Journal of Public Health
https://www.readbyqxmd.com/read/28727280/two-novel-effectors-of-trafficking-and-maturation-of-the-yeast-plasma-membrane-h-atpase
#11
Yosef Geva, Jonathan Crissman, Eric C Arakel, Natalia Gómez-Navarro, Silvia G Chuartzman, Kyle R Stahmer, Blanche Schwappach, Elizabeth A Miller, Maya Schuldiner
The endoplasmic reticulum (ER) is the entry site of proteins into the endomembrane system. Proteins exit the ER via COPII vesicles in a selective manner, mediated either by direct interaction with the COPII coat or aided by cargo receptors. Despite the fundamental role of such receptors in protein sorting, only a few have been identified. To further define the machinery that packages secretory cargo and targets proteins from the ER to Golgi membranes, we used multiple systematic approaches, which revealed two uncharacterized proteins that mediate the trafficking and maturation of Pma1, the essential yeast plasma membrane proton ATPase...
July 20, 2017: Traffic
https://www.readbyqxmd.com/read/28726934/clove-extract-functions-as-a-natural-fatty-acid-synthesis-inhibitor-and-prevents-obesity-in-a-mouse-model
#12
Yiran Ding, Zhennan Gu, Yihe Wang, Shunhe Wang, Haiqin Chen, Hao Zhang, Wei Chen, Yong Q Chen
Numerous medicinal plants have been reported to prevent various chronic diseases. In this study, we screened a new FASN inhibitor-alcohol extract of clove (AEC) using a fast microplate method developed in our laboratory. The major components of AEC were: eugenol (42.27%), acetyl eugenol (29.12%), caryophyllene (15.40%), and humulene (3.22%). Fatty acid synthase (FASN) is a key enzyme for de novo lipogenesis, and it has been suggested as a potential therapeutic target in cancer and obesity. We have tested the ability of AEC to inhibit FASN in mammalian cells and tissues...
July 20, 2017: Food & Function
https://www.readbyqxmd.com/read/28722226/biology-of-slac1-type-anion-channels-from-nutrient-uptake-to-stomatal-closure
#13
REVIEW
Rainer Hedrich, Dietmar Geiger
Contents 1 I. 1 II. 2 III. 5 IV. 8 V. 11 VI. 12 13 13 References 13 SUMMARY: Stomatal guard cells control leaf CO2 intake and concomitant water loss to the atmosphere. When photosynthetic CO2 assimilation is limited and the ratio of CO2 intake to transpiration becomes suboptimal, guard cells, sensing the rise in CO2 concentration in the substomatal cavity, deflate and the stomata close. Screens for mutants that do not close in response to experimentally imposed high CO2 atmospheres identified the guard cell-expressed Slowly activating anion channel, SLAC1, as the key player in the regulation of stomatal closure...
July 19, 2017: New Phytologist
https://www.readbyqxmd.com/read/28722114/uv-b-irradiation-differentially-regulates-terpene-synthases-and-terpene-content-of-peach
#14
Hongru Liu, Xiangmei Cao, Xiaohong Liu, Rui Xin, Jiaojiao Wang, Jie Gao, Boping Wu, Liuxiao Gao, Changjie Xu, Bo Zhang, Donald Grierson, Kunsong Chen
Plants generate protective molecules in response to UV light. In laboratory experiments, 48 h UV-B irradiation of peach fruit and leaves reduced the flavor-related monoterpene linalool by 60 %. No isoprene was detected but other terpenoids increased significantly, including a 3-fold accumulation of the sesquiterpene (E,E)-α-farnesene, which was also increased by jasmonic acid treatment. RNA-sequencing revealed altered transcript levels for two terpene synthases: PpTPS1, a TPS-g subfamily member, decreased by 86 % and PpTPS2, a TPS-b subfamily member, increased 80-fold...
July 19, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28719876/photoelectrocatalytic-degradation-of-methylene-blue-using-f-doped-tio2-photoelectrode-under-visible-light-irradiation
#15
Dong Liu, Renwen Tian, Jianqiao Wang, Er Nie, Xianqing Piao, Xin Li, Zhuo Sun
Photoelectrocatalysis (PEC) has attracted great interest due to cost effectiveness and high efficiency in water treatment. In this study, F doped TiO2 (F-TiO2) photoelectrodes with honeycomb like morphology were prepared, and the PEC performance was investigated. F-TiO2 particles that showed enhanced absorption of visible light were synthesized via a sol-gel method. F-TiO2 particles were anchored onto the surface of F-doped SnO2 glass by a screen-printing method to prepare the F-TiO2 photoelectrodes. The PEC performance of the F-TiO2 photoelectrodes was investigated via the degradation of methylene blue (MB) under visible light irradiation...
July 14, 2017: Chemosphere
https://www.readbyqxmd.com/read/28719195/fluorinated-boronic-acid-appended-pyridinium-salts-and-19f-nmr-spectroscopy-for-diol-sensing
#16
Jörg Axthelm, Sven H C Askes, Martin Elstner, Upendar Reddy Gandra, Helmar Görls, Peter Bellstedt, Alexander Schiller
The identification and discrimination of diols is of fundamental importance in medical diagnostics, such as measuring the contents of glucose in the urine of diabetes patients. Diol sensors are often based on fluorophore-appended boronic acids, but these severely lack discrimination power and their response is one-dimensional. As an alternative strategy, we present the use of fluorinated boronic acid-appended pyridinium salts in combination with 19F NMR spectroscopy. A pool of 59 (bio)analytes was screened, containing monosaccharides, phosphorylated and N-acetylated sugars, polyols, carboxylic acids, nucleotides and amines...
July 18, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28716025/beta-mangostin-demonstrates-apoptogenesis-in-murine-leukaemia-wehi-3-cells-in-vitro-and-in-vivo
#17
Fatima Abdelmutaal Ahmed Omer, Najihah Mohd Hashim, Mohamed Yousif Ibrahim, Abdulmannan F Aldoubi, Pouya Hassandarvish, Firouzeh Dehghan, Noraziah Nordin, Hamed Karimian, Landa Zeenelabdin Ali Salim, Mahmood Ameen Abdulla, Karim Al-Jashamy, Syam Mohan
BACKGROUND: Beta-mangostin (BM) is a xanthone-type of natural compound isolated from Cratoxylum arborescens. This study aimed to examine the apoptosis mechanisms induced by BM in a murine monomyelocytic cell line (WEHI-3) in vitro and in vivo. METHODS: A WEHI-3 cell line was used to evaluate the cytotoxicity of BM by MTT. AO/PI and Hoechst 33342 dyes, Annexin V, multiparametric cytotoxicity 3 by high content screening (HCS); cell cycle tests were used to estimate the features of apoptosis and BM effects...
July 17, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28710451/selection-of-complementary-foods-based-on-optimal-nutritional-values
#18
Partho Sen, Adil Mardinogulu, Jens Nielsen
Human milk is beneficial for growth and development of infants. Several factors result in mothers ceasing breastfeeding which leads to introduction of breast-milk substitutes (BMS). In some communities traditional foods are given as BMS, in others they are given as complementary foods during weaning. Improper food selection at this stage is associated with a high prevalence of malnutrition in children under 5 years. Here we listed the traditional foods from four continents and compared them with human milk based on their dietary contents...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28710376/identification-and-expression-of-cysteine-sulfinate-decarboxylase-possible-regulation-of-taurine-biosynthesis-in-crassostrea-gigas-in-response-to-low-salinity
#19
Xuelin Zhao, Qi Li, Qian Meng, Chenyang Yue, Chengxun Xu
Taurine has been reported high amounts in marine animals to maintain osmotic balance between osmoformers and sea water. Approximately 80% of the total amino-acid content is taurine in Pacific oyster Crassostrea gigas, an intertidal and euryhaline species. In this study, we cloned the two copies of cysteine sulfinate decarboxylase (CSAD), the key enzyme in taurine biosynthesis pathway, screened in oyster genome data. Sequentially, we compared the expression patterns of CgCSAD1 and CgCSAD2 under low salinity treatment (8‰ and 15‰) using different families from two populations...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28710351/combination-therapy-for-tuberculosis-treatment-pulmonary-administration-of-ethionamide-and-booster-co-loaded-nanoparticles
#20
Joana Costa-Gouveia, Elisabetta Pancani, Samuel Jouny, Arnaud Machelart, Vincent Delorme, Giuseppina Salzano, Raffaella Iantomasi, Catherine Piveteau, Christophe J Queval, Ok-Ryul Song, Marion Flipo, Benoit Deprez, Jean-Paul Saint-André, José Hureaux, Laleh Majlessi, Nicolas Willand, Alain Baulard, Priscille Brodin, Ruxandra Gref
Tuberculosis (TB) is a leading infectious cause of death worldwide. The use of ethionamide (ETH), a main second line anti-TB drug, is hampered by its severe side effects. Recently discovered "booster" molecules strongly increase the ETH efficacy, opening new perspectives to improve the current clinical outcome of drug-resistant TB. To investigate the simultaneous delivery of ETH and its booster BDM41906 in the lungs, we co-encapsulated these compounds in biodegradable polymeric nanoparticles (NPs), overcoming the bottlenecks inherent to the strong tendency of ETH to crystallize and the limited water solubility of this Booster...
July 14, 2017: Scientific Reports
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