keyword
MENU ▼
Read by QxMD icon Read
search

High content screening

keyword
https://www.readbyqxmd.com/read/28931315/the-liver-toxicity-knowledge-base-lktb-and-drug-induced-liver-injury-dili-classification-for-assessment-of-human-liver-injury
#1
Shraddha Thakkar, Weida Tong, Minjun Chen, Hong Fang, Zhichao Liu, Ruth Roberts
Drug-induced liver injury (DILI) is challenging drug development, clinical practice and regulation. The Liver Toxicity Knowledge Base (LTKB) provides essential data for DILI study. Areas Covered: The LTKB provided various types of data that can be used to assess and predict DILI. Among much information available, several reference drug lists with annotated human DILI risk are of important. The LTKB DILI classification data include DILI severity concern determined by the FDA drug labeling, DILI severity score from the NIH LiverTox database, and other DILI classification schemes from the literature...
September 21, 2017: Expert Review of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/28930984/high-fat-diet-feeding-and-high-throughput-triacylglyceride-assay-in-drosophila-melanogaster
#2
Soda Balla Diop, Ryan T Birse, Rolf Bodmer
Heart disease is the number one cause of human death worldwide. Numerous studies have shown strong connections between obesity and cardiac malfunction in humans, but more tools and research efforts are needed to better elucidate the mechanisms involved. For over a century, the genetically highly tractable model of Drosophila has been instrumental in the discovery of key genes and molecular pathways that proved to be highly conserved across species. Many biological processes and disease mechanisms are functionally conserved in the fly, such as development (e...
September 13, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28930217/influence-of-different-isolation-methods-on-chemical-composition-and-bioactivities-of-the-fruit-peel-oil-of-citrus-medica-l-var-sarcodactylis-noot-swingle
#3
Gang Deng, Jonathan D Craft, Kelly Marie Steinberg, Pei Lei Li, Suraj Kumar Pokharel, William N Setzer
Background: The chemical composition and bioactivities of essential oils (EOs) of fingered citron (Citrus medica L. var. sarcodactylis (Noot.) Swingle) are considerably sensitive and lapsible during high-temperature processing of traditional separating techniques. In the present research, vacuum distillation and ultrafiltration were utilized in order to process the concentrated juice from fingered citron, obtaining a high-quality essential oil. Methods: In order to compare the essential oils obtained by conventional means, the chemical compositions of the essential oils were analyzed using GC-MS, before antimicrobial and antioxidant screening assays were carried out...
January 4, 2017: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/28929829/quantitative-sensory-testing-for-assessment-of-somatosensory-function-in-human-oral-mucosa-a-review
#4
Pin Zhou, Yaming Chen, Jinglu Zhang, Kelun Wang, Peter Svensson
OBJECTIVE: This narrative review provides an overview of the quantitative sensory testing (QST) to assess somatosensory function in human oral mucosa. MATERIAL AND METHODS: A literature search was conducted in the PubMed database to identify studies in vivo on human oral mucosa using QST methods. A list of 149 articles was obtained and screened. A total of 36 relevant articles remained and were read in full text. Manual search of the reference lists identified eight additional relevant studies...
September 20, 2017: Acta Odontologica Scandinavica
https://www.readbyqxmd.com/read/28928993/what-drives-embryo-development-chromosomal-normality-or-mitochondria
#5
A Bayram, I Elkhatib, A Arnanz, A Linan, F Ruiz, B Lawrenz, H M Fatemi
OBJECTIVE: To report the arrest of euploid embryos with high mtDNA content. DESIGN: A report of 2 cases. SETTING: Private fertility clinic. PATIENTS: 2 patients, 45 and 40 years old undergoing IVF treatment. INTERVENTIONS: Mature oocytes were collected and vitrified from two ovarian stimulations. Postthaw, survived mature oocytes underwent fertilization by intracytoplasmic sperm injection (ICSI)...
2017: Case Reports in Genetics
https://www.readbyqxmd.com/read/28928109/components-and-outcomes-of-internet-based-interventions-for-caregivers-of-older-adults-systematic-review
#6
REVIEW
Cassioppée Guay, Claudine Auger, Louise Demers, W Ben Mortenson, William C Miller, Dominique Gélinas-Bronsard, Sara Ahmed
BACKGROUND: When trying to access interventions to improve their well-being and quality of life, family caregivers face many challenges. Internet-based interventions provide new and accessible opportunities to remotely support them and can contribute to reducing their burden. However, little is known about the link existing between the components, the use of behavior change techniques, and the outcomes of these Internet-based interventions. OBJECTIVE: This study aimed to provide an update on the best available evidence about the efficacy of Internet-based interventions for caregivers of older adults...
September 19, 2017: Journal of Medical Internet Research
https://www.readbyqxmd.com/read/28924673/super-resolution-high-content-screening-and-analysis
#7
K Soliman
Revealing the subcellular phenotypes at the molecular scale is critical to understand the mechanisms by which the cells function and respond to chemical treatments. Super-resolution microscopy and robust analysis tools enabled biologists to reveal and quantify phenotypes at unprecedented resolution. Developing automated imaging analysis solutions for super-resolution imaging will make high-content-screening (HCS) applicable for super-resolution microscopy, which will give access to new complex information. Here, I provide an instant automated analysis procedure for analyzing super-resolution images via CellProfiler ( www...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28924210/kinase-inhibitor-screening-using-artificial-neural-networks-and-engineered-cardiac-biowires
#8
Genevieve Conant, Samad Ahadian, Yimu Zhao, Milica Radisic
Kinase inhibitors are often used as cancer targeting agents for their ability to prevent the activation of cell growth and proliferation signals. Cardiotoxic effects have been identified for some marketed kinase inhibitors that were not detected during clinical trials. We hypothesize that more predictive cardiac functional assessments of kinase inhibitors on human myocardium can be established by combining a high-throughput two-dimensional (2D) screening assay and a high-content three-dimensional (3D) engineered cardiac tissue (Biowire(TM)) based assay, and using human induced pluripotent stem cell-derived CMs (hiPSC-CMs)...
September 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28922619/identification-of-angiogenesis-inhibitors-using-a-co-culture-cell-model-in-a-high-content-and-high-throughput-screening-platform
#9
Shuaizhang Li, Chia-Wen Hsu, Srilatha Sakamuru, Chaozhong Zou, Ruili Huang, Menghang Xia
Angiogenesis is an important hallmark of cancer, contributing to tumor formation and metastasis. In vitro angiogenesis models for analyzing tube formation serve as useful tools to study these processes. However, current in vitro co-culture models using primary cells have limitations in usefulness and consistency. Therefore, in the present study, an in vitro co-culture assay system was optimized in a 1536-well format for high-throughput screening using human telomerase reverse transcriptase (hTERT)-immortalized mesenchymal stem cells and aortic endothelial cells...
September 1, 2017: SLAS Technology
https://www.readbyqxmd.com/read/28920118/hybrid-magnetic-iron-oxide-nanoparticles-with-tunable-field-directed-self-assembly
#10
Vikash Malik, Antara Pal, Olivier Pravaz, Jérôme J Crassous, Simon Granville, Bernard Grobety, Ann M Hirt, Hervé Dietsch, Peter Schurtenberger
We describe the synthesis of hybrid magnetic ellipsoidal nanoparticles that consist of a mixture of two different iron oxide phases, hematite (α-Fe2O3) and maghemite (γ-Fe2O3), and characterize their magnetic field-driven self-assembly. We demonstrate that the relative amount of the two phases can be adjusted in a continuous way by varying the reaction time during the synthesis, leading to strongly varying magnetic properties of the particles. Not only does the saturation magnetization increase dramatically as the composition of the spindles changes from hematite to maghemite, but also the direction of the induced magnetic moment changes from being parallel to the short axis of the spindle to being perpendicular to it...
September 18, 2017: Nanoscale
https://www.readbyqxmd.com/read/28918139/comparison-of-milk-protein-composition-and-rennet-coagulation-properties-in-native-swedish-dairy-cow-breeds-and-high-yielding-swedish-red-cows
#11
Nina A Poulsen, Maria Glantz, Anette K Rosengaard, Marie Paulsson, Lotte B Larsen
Recent studies have reported a very high frequency of noncoagulating milk in Swedish Red cows. The underlying factors are not fully understood. In this study, we explored rennet-induced coagulation properties and relative protein profiles in milk from native Swedish Mountain and Swedish Red Polled cows and compared them with a subset of noncoagulating (NC) and well-coagulating (WC) milk samples from modern Swedish Red cows. The native breeds displayed a very low prevalence of NC milk and superior milk coagulation properties compared with Swedish Red cows...
September 13, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28916948/viewer-discretion-advised-is-youtube-a-friend-or-foe-in-surgical-education
#12
H Alejandro Rodriguez, Monica T Young, Hope T Jackson, Brant K Oelschlager, Andrew S Wright
BACKGROUND: In the current era, trainees frequently use unvetted online resources for their own education, including viewing surgical videos on YouTube. While operative videos are an important resource in surgical education, YouTube content is not selected or organized by quality but instead is ranked by popularity and other factors. This creates a potential for videos that feature poor technique or critical safety violations to become the most viewed for a given procedure. METHODS: A YouTube search for "Laparoscopic cholecystectomy" was performed...
September 15, 2017: Surgical Endoscopy
https://www.readbyqxmd.com/read/28914485/match-of-psychosocial-risk-and-psychosocial-care-in-families-of-a-child-with-cancer
#13
S M Sint Nicolaas, S A Schepers, E M M van den Bergh, Y de Boer, I Streng, E M van Dijk-Lokkart, M A Grootenhuis, C M Verhaak
OBJECTIVE: The Psychosocial Assessment Tool (PAT) was developed to screen for psychosocial risk, aimed to be supportive in directing psychosocial care to families of a child with cancer. This study aimed to determine (i) the match between PAT risk score and provided psychosocial care with healthcare professionals blind to outcome of PAT assessment, and (ii) the match between PAT risk score and team risk estimation. METHODS: Eighty-three families of children with cancer from four pediatric oncology centers in the Netherlands participated (59% response rate)...
September 15, 2017: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/28911653/assessment-of-rosehips-based-on-the-content-of-their-biologically-active-compounds
#14
Apurva Bhave, Vera Schulzova, Hana Chmelarova, Libor Mrnka, Jana Hajslova
In this study, an in-depth analysis of the unique set of rosehip samples from 71 Rosa genotypes was conducted with the aim to identify the most suitable ones for applications in the food and pharmaceutical industries based on the content of biologically active compounds. In the first part of our experiments, the antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl assay and the genotypes with the highest values were selected for the follow-up analysis. In the second part of experiments, the major classes of biologically active compounds in rosehips such as carotenoids, tocopherols, flavonoids, and triterpenoic acids were further quantified using liquid chromatography-based techniques...
July 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28903629/a-multidisciplinary-approach-for-developing-an-assessment-tool-for-touch-screen-devices
#15
Alexandra Danial-Saad, Lorenzo Chiari
AIM: The aim of this study was to describe the processes of reaching consensus regarding the assessment of the user's skills required to operate various touch screen devices. A five-step procedure was used to collect and validate the required skills by a multidisciplinary team of 52 experts. Content validity was calculated to determine the agreement levels between the experts. A comparison was made between the discipline groups in order to test correlation between each group and their choice of specific clusters of tasks...
September 14, 2017: Disability and Rehabilitation. Assistive Technology
https://www.readbyqxmd.com/read/28903093/chlorination-chloramination-and-ozonation-of-carbamazepine-enhance-cytotoxicity-and-genotoxicity-multi-endpoint-evaluation-and-identification-of-its-genotoxic-transformation-products
#16
Yingnan Han, Mei Ma, Na Li, Rui Hou, Chao Huang, Yoshimitsu Oda, Zijian Wang
Investigations have focused on the removal and transformation of pharmaceuticals during drinking water and wastewater treatment. In the present study, we investigated for the first time the changes of the cytotoxicity and genotoxicity based on different modes of action (MoAs) during chlorination, chloramination and ozonation processes of the anti-epileptic drug carbamazepine (CBZ). The results illustrated that ozonation enhanced the cytotoxicity and the chromosome damage effects on CHO-K1 cells detected by cytokinesis-block micronucleus (CBMN) assay based on high-content screening technique, though ozonation showed the highest removal efficiency for CBZ...
September 1, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28900089/-high-content-sirna-screen-of-the-kinome-identifies-kinases-involved-in-git2-induced-mesenchymal-epithelial-transition
#17
M G Cao, J Xu, Q F Yang, Z P Guo, K B Zhang, X-B Li, S Q Wu, W Zhou
Epithelial-mesenchymal transition (EMT) and its reverse process mesenchymal-epithelial transition (MET) programs are involced in the metastatic process. More and more evidence confirms that EMT is vital for the initiation and dissemination of cancer cells whereas MET is critical for successful metastatic colonization of a secondary organ. The regulating mechanism of EMT mediated cancer progression and metastasis has been deeply investigated. However, what processes are dependent on MET in metastatic cascades remains unclear...
July 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/28899711/colorectal-cancer-screening-associations-between-information-provision-attitudes-and-intended-participation
#18
Stephanie D Brandhof, Angela Fagerlin, Sarah Hawley, Esther Toes-Zoutendijk, Lyndal Trevena, Kirsten McCaffery, Ida J Korfage
OBJECTIVE: Properly informing target audiences is crucial in population-based screening programs. We aimed to evaluate the impact of information about CRC screening on attitudes and intended participation in a screening-naive population. METHODS: 614 persons (aged 55-75 years) received a survey. Information on CRC and screening was provided piece by piece, and per piece its impact on attitudes and intended participation was assessed. All persons received the same information content, but the sequence of information differed per condition: information on the high mortality rate of colorectal cancer was presented in the first or the second piece...
September 9, 2017: Patient Education and Counseling
https://www.readbyqxmd.com/read/28899388/a-review-of-grey-and-academic-literature-of-evaluation-guidance-relevant-to-public-health-interventions
#19
Sarah Denford, Charles Abraham, Margaret Callaghan, Peter Aighton, Frank De Vocht, Steven Arris
BACKGROUND: Public Health evaluation is essential to understanding what does and does not work, and robust demonstration of effectiveness may be crucial to securing future funding. Despite this, programs are often implemented with poor, incomplete or no evaluation. Public health practitioners are frequently required to provide evidence for the effectiveness of their services; thus, there is a growing need for evaluation guidance on how to evaluate public health programs. The aim of this study is to identify accessible high-quality, evaluation guidance, available to researchers and practitioners and to catalogue, summarise and categorise the content of a subset of accessible, quality guides to evaluation...
September 12, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/28895613/-the-g-score-a-short-questionnaire-for-the-measurement-of-subjective-physical-health
#20
Juliana Schneider, Markus Zenger, Elmar Brähler, Yve Stöbel-Richter, Hendrik Berth
Aim The measurement of subjective physical health is important in clinical settings as well as for research purposes. In the present paper, the psychometric properties of the G-Score, a 4 item screening questionnaire for the self-assessment of one's physical health, is explored. Methods The Objectivity, validity and reliability were estimated. Moreover, an item analysis and a suitable cut-off-value for the differentiation between healthy and presumably ill subjects were conducted. Data from the Saxony Longitudinal Study ("Sächsische Längsschnittstudie") 1998-2013 were analysed (N=324-417 healthy individuals)...
September 11, 2017: Psychotherapie, Psychosomatik, Medizinische Psychologie
keyword
keyword
16674
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"