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Colitis mice

Irina Tcymbarevich, Shola M Richards, Giancarlo Russo, Jelena Kühn-Georgijevic, Jesus Cosin-Roger, Katharina Baebler, Silvia Lang, Susan Bengs, Kirstin Atrott, Carla Bettoni, Sven Gruber, Isabelle Frey-Wagner, Michael Scharl, Benjamin Misselwitz, Carsten A Wagner, Klaus Seuwen, Gerhard Rogler, Pedro A Ruiz, Marianne Spalinger, Cheryl de Vallière
Background: Tissue inflammation in inflammatory bowel diseases [IBD] is associated with local acidification. Genetic variants in the pH-sensing G protein-coupled receptor 65, also known as T cell death-associated gene 8 [TDAG8], have been implicated in IBD and other autoimmune diseases. Since the role of TDAG8 in intestinal inflammation remains unclear, we investigated the function of TDAG8 using murine colitis models. Methods: The effects of TDAG8 deficiency were assessed in dextran sodium sulphate [DSS], IL-10-/-, and T cell transfer colitis murine models...
October 9, 2018: Journal of Crohn's & Colitis
Seung-Woo Han, Jeong Min Kim, Yunmee Lho, Hyun Jung Cho, Youn-Kwan Jung, Jung-Ae Kim, Hoyul Lee, Yu-Jeong Lee, Eun Soo Kim
Background: Adhesion molecules maintain the intestinal barrier function that is crucial to prevent intestinal inflammation. Dual immunoglobulin domain-containing adhesion molecule (DICAM) has been recently identified and known for the involvement in cell-cell adhesion through homophilic interaction and heterophilic interaction with integrin αVβ3. We tested whether the change of DICAM expression affects the severity of colonic inflammation. Methods: Colitis was induced with oral administration of 2...
December 9, 2018: Inflammatory Bowel Diseases
Eleni Christodoulou-Vafeiadou, Fotis Ioakeimidis, Margarita Andreadou, Giorgos Giagkas, George Stamatakis, Martin Reczko, Martina Samiotaki, Anastasios D Papanastasiou, Ioannis Karakasiliotis, Dimitris L Kontoyiannis
HuR is an abundant RNA-binding protein acting as a post-transcriptional regulator of many RNAs including mRNAs encoding inflammatory mediators, cytokines, death signalers and cell cycle regulators. In the context of intestinal pathologies, elevated HuR is considered to enhance the stability and the translation of pro-tumorigenic mRNAs providing the rationale for its pharmacological targeting. However, HuR also possesses specific regulatory functions for innate immunity and cytokine mRNA control which can oppose intestinal inflammation and tumor promotion...
2018: Frontiers in Immunology
Leticia Tordesillas, Daniel Lozano-Ojalvo, David Dunkin, Lucie Mondoulet, Judith Agudo, Miriam Merad, Hugh A Sampson, M Cecilia Berin
The skin immune system must discriminate between innocuous antigens and pathogens. Antigen applied topically using a Viaskin® patch elicits immune tolerance that can suppress colitis and food allergy. Here we show how topical antigen is acquired and presented by dendritic cells in the skin. Topical antigen is acquired by Langerhans cells (LC) and CD11b+ cDC2s but not cDC1s, and both  LCs and CD11b+ cDC2s reaching the lymph node can prime T cells and expand LAP+ Tregs. However, LCs are neither required nor sufficient for T cell priming, and have no role in tolerance induction...
December 7, 2018: Nature Communications
John Tam, Therwa Hamza, Bing Ma, Kevin Chen, Greg L Beilhartz, Jacques Ravel, Hanping Feng, Roman A Melnyk
Clostridium difficile is the leading cause of nosocomial diarrhea and colitis in the industrialized world. Disruption of the protective gut microbiota by antibiotics enables colonization by multidrug-resistant C. difficile, which secrete up to three different protein toxins that are responsible for the gastrointestinal sequelae. Oral agents that inhibit the damage induced by toxins, without altering the gut microbiota, are urgently needed to prevent primary disease and break the cycle of antibiotic-induced disease recurrence...
December 7, 2018: Nature Communications
Ayman Hyder
Nutrients may modulate immunity through their transcription factors that act on both metabolic and immunity genes. It has been shown that the transcription factor of lipid ligands PPARγ physically binds the gene promoter of the peptidoglycan recognition protein (PGlyRP3), which showed anti-inflammatory action in vitro. It is hypothesized in the present work that olive oil feeding protects against toxicity of DSS-induced colitis via activation of the lipid transcription factor PPARγ that stimulates the anti-inflammatory PGlyRP3...
December 7, 2018: International Immunopharmacology
Tianyong Hu, Wenhui Hu, Li Ma, Xianhai Zeng, Jiangqi Liu, Baohui Cheng, Pingchang Yang, Shuqi Qiu, Gui Yang, Donghui Chen, Zhiqiang Liu
AIM: To investigate whether the intrarectal administration of the ubiquitin E3 ligase A20 (A20) attenuates intestinal inflammation and influences regulatory T cells in experimental colitis. METHODS: A dextran sulfate sodium induced chronic colitis mouse model was established. The symptoms and manifestations of colitis and the severity of colonic mucosal inflammation were evaluated. The protective role of A20 expression in the intestine was analyzed after the administration of a pVAX1-A20 recombinant eukaryotic vector, which was encapsulated into poly(L-lactide-co-glycolide) as a nanoparticle...
November 17, 2018: Digestive and Liver Disease
Shizhen Dong, Menglu Chen, Faliang Dai, Qingxia Xuan, Pan Chen, Dandan Feng, Lei Gao, Chendi Zhu, Yongchao Chang, Fong-Fong Chu, Qiang Gao
AIM: This study investigated the impact of 5-hydroxytryptamine (5-HT) on the expression of NOXs in dextran sulfate sodium (DSS)-induced colitis in mice. METHODS: C57BL/6J (B6) mice at 6 to 8 weeks of age were treated with 5-HT, DSS, or DSS + 5-HT. After 6-day treatment, the severity of colitis, infiltration of leukocytes, and messenger RNA (mRNA) and/or protein levels of Nox1, Nox2, Nox4, and Duox2 were analyzed in the colon by real-time quantitative polymerase chain reaction (qPCR), immunohistochemistry (IHC), and Western blot analysis...
December 7, 2018: Journal of Cellular Biochemistry
Snur Ma Hassan, Ali Hussein Hassan
Objectives: This study aimed to investigate the effect of Shogaol on dextran sodium sulfate (DSS)- induced ulcerative colitis (UC) in mice compared to an immune-suppressant chemotherapeutic medicine, known as 6-thioguanine (6-TG). Materials and Methods: Thirty-six adult BALB/c mice were divided into six groups: group 1 (positive control): no DSS exposure and no treatment; group 2 (negative control): DSS exposure without treatment; group 3 (vehicle control): DSS exposure and olive oil treatment; group 4: DSS exposure and 0...
September 2018: Iranian Journal of Basic Medical Sciences
Rogatien Charlet, Clovis Bortolus, Melissandre Barbet, Boualem Sendid, Samir Jawhara
Background: The intestinal microbiota plays a crucial role in the maintenance of gut homeostasis. Changes in crosstalk between the intestinal epithelial cells, immune cells and the microbiota are critically involved in the development of inflammatory bowel disease. In the experimental mouse model, the development of colitis induced by dextran sulfate sodium (DSS) promotes overgrowth of the opportunistic yeast pathogen Candida glabrata . Conversely, fungal colonization aggravates inflammatory parameters...
2018: Gut Pathogens
Daniel A Giles, Sonja Zahner, Petra Krause, Esmé Van Der Gracht, Thomas Riffelmacher, Venetia Morris, Alexei Tumanov, Mitchell Kronenberg
Over 1.5 million individuals in the United States are afflicted with inflammatory bowel disease (IBD). While the progression of IBD is multifactorial, chronic, unresolved inflammation certainly plays a key role. Additionally, while multiple immune mediators have been shown to affect pathogenesis, a comprehensive understanding of disease progression is lacking. Previous work has demonstrated that a member of the TNF superfamily, TNFSF14 (LIGHT), which is pro-inflammatory in several contexts, surprisingly plays an important role in protection from inflammation in mouse models of colitis, with LIGHT deficient mice having more severe disease pathogenesis...
2018: Frontiers in Immunology
Fabiany DA Costa Gonçalves, Michele Aramburu Serafini, Helena Flores Mello, Bianca Pfaffenseller, Anelise Bergmann Araújo, Fernanda Visioli, Ana Helena Paz
BACKGROUND AIMS: Although mesenchymal stromal cells (MSCs) have shown therapeutic potential in intestinal tissue repair, controversy concerning their short survival and poor biodistribution in recipient tissues still remains. Therefore, we investigated the paracrine role of MSC in three-dimensional culture of colon with experimental colitis. METHODS: Colitis was induced in mice by oral administration of dextran sulfate sodium (DSS) for 7 days. Inflammatory responses were assessed on the basis of clinical signs, morphological, and histopathological parameters...
December 2018: Cytotherapy
Teresa Vezza, Francesca Algieri, José Garrido-Mesa, María Pilar Utrilla, María Elena Rodríguez-Cabezas, Alberto Baños, Enrique Guillamón, Federico García, Alba Rodríguez-Nogales, Julio Gálvez
SCOPE: Propyl-propane thiosulfonate (PTSO) is a component isolated from garlic (Allium sativum) with antioxidant, anti-inflammatory, immunomodulatory and antimicrobial properties. In consequence, PTSO could be a potential candidate for the treatment of inflammatory bowel diseases. METHODS AND RESULTS: The anti-inflammatory effects of PTSO were studied in two mice models of colitis: 2,4-dinitrobenzene sulfonic acid (DNBS) (PTSO doses: 0.01-10 mg/kg) and dextran sodium sulfate (DSS) (PTSO doses: 0...
December 5, 2018: Molecular Nutrition & Food Research
Ahmed Ibrahim, Luisa Hugerth, Linnea Hases, Ashish Saxena, Maike Seifert, Quentin Thomas, Jan-Åke Gustafsson, Lars Engstrand, Cecilia Williams
Chronic inflammation of the colon (colitis) is a risk factor for colorectal cancer (CRC). Hormone-replacement therapy reduces CRC incidences, and the estrogen receptor beta (ERβ/ESR2) has been implicated in this protection. Gut microbiota is altered in both colitis and CRC and may influence the severity of both. Here we test the hypothesis that intestinal ERβ impacts the gut microbiota. Mice with and without intestine-specific deletion of ERβ (ERβKOVil ) were generated using the Cre-LoxP system. Colitis and CRC were induced with a single intraperitoneal injection of azoxymethane (AOM) followed by administration of three cycles of dextran sulfate sodium (DSS) in drinking water...
December 4, 2018: International Journal of Cancer. Journal International du Cancer
Marina Liso, Stefania De Santis, Aurelia Scarano, Giulio Verna, Manuela Dicarlo, Vanessa Galleggiante, Pietro Campiglia, Mauro Mastronardi, Antonio Lippolis, Mirco Vacca, Anastasia Sobolewski, Grazia Serino, Eugenio Butelli, Maria De Angelis, Cathie Martin, Angelo Santino, Marcello Chieppa
Inflammatory bowel diseases (IBD) are debilitating chronic inflammatory disorders that develop as a result of a defective immune response toward intestinal bacteria. Intestinal dysbiosis is associated with the onset of IBD and has been reported to persist even in patients in deep remission. We investigated the possibility of a dietary-induced switch to the gut microbiota composition using Winnie mice as a model of spontaneous ulcerative colitis and chow enriched with 1% Bronze tomato. We used the near isogenic tomato line strategy to investigate the effects of a diet enriched in polyphenols administered to mild but established chronic intestinal inflammation...
December 2, 2018: Nutrients
Hiroki Kiyohara, Tomohisa Sujino, Toshiaki Teratani, Kentaro Miyamoto, Mari Mochizuki Arai, Ena Nomura, Yosuke Harada, Ryo Aoki, Yuzo Koda, Yohei Mikami, Shinta Mizuno, Makoto Naganuma, Tadakazu Hisamatsu, Takanori Kanai
Background & Aims: Psoriasis and inflammatory bowel disease (IBD) are both chronic inflammatory diseases occurring in the skin and gut, respectively. It is well established that psoriasis and IBD have high concordance rates, and similar changes in immune cells and microbiome composition have been reported in both conditions. To study this connection, we used a combination murine model of psoriatic dermatitis and colitis in which mice were treated topically with the Toll-like receptor 7 agonist imiquimod (IMQ) and fed dextran sulfate sodium (DSS)...
2019: Cellular and Molecular Gastroenterology and Hepatology
Xin Tun, Keiji Yasukawa, Ken-Ichi Yamada
Ulcerative colitis is characterized by colonic mucosal bleeding and ulceration, often with repeated active and remission stages. One factor in ulcerative colitis development is increased susceptibility to commensal bacteria and lipopolysaccharide (LPS). LPS activates macrophages to release nitric oxide (NO) through Toll-like receptor 4 (TLR4) signaling. However, whether NO is beneficial or detrimental to colitis remains controversial. In this study, we investigated whether NO enhances the development of colitis in mice treated with dextran sulfate sodium (DSS) and inflammation in cells treated with low-dose LPS...
2018: Biological & Pharmaceutical Bulletin
Hongce Chen, Wuyu Fu, Hongyuan Chen, Siyuan You, Xiawan Liu, Yujiao Yang, Yao Wei, Jun Huang, Wen Rui
Magnolol is a natural extract and the main bioactive component from Chinese medicine-Magnolia. We speculate that it's functional action might be associated with the anti-inflammatory effects of magnolol. Herein, the main purpose was to elucidate the phagocytic immune function and anti-inflammatory activities associated. The toxicity of magnolol on U937 and LO-2 cells was assayed by MTT, flow cytometry and laser scanning confocal microscope was utilized to detect the phagocytosis effect on U937 cells, C57BL/6 mice and the follow-up hematoxylin-eosin staining methods were used to evaluate its bioactivity in vivo...
November 27, 2018: Molecular Immunology
Rongjuan Zheng, Jiaheng Ma, Dan Wang, Wenxiao Dong, Sinan Wang, Tianyu Liu, Runxiang Xie, Li Liu, Bangmao Wang, Hailong Cao
Inflammatory bowel disease (IBD), characterized by sustained inflammation, is a latent risk factor of colon tumorigenesis. Silibinin has been reported to be anti-inflammatory and antineoplastic, but its efficacy on colitis-associated cancer (CAC) has not been reported. Interlukin-6/signal transducer and activator of transcription 3 (IL-6/STAT3) is the key signaling pathway involved in CAC. We evaluated the chemopreventive effect of silibinin on a CAC mouse model and determined its impact on IL-6/STAT3 signaling...
2018: Mediators of Inflammation
Chu-Tian Mai, Mei-Mei Wu, Chun-Li Wang, Zi-Ren Su, Yuan-Yuan Cheng, Xiao-Jun Zhang
Activation of NLRP3 inflammasomes is crucial in the pathological process of Ulcerative colitis (UC), which could be negatively regulated by PINK1/Parkin-driven mitophagy. Palmatine is a herb derived isoquinoline alkaloid with potent anti-inflammatory and anti-bacteria activities. In present study, we evaluated the effect of palmatine on dextran sulfate sodium (DSS)-induced mice colitis and examined whether its effect is exerted by promoting mitophagy-mediated NLRP3 inflammasome inactivation. The result showed that palmatine (40, 100 mg/kg) significantly prevented bodyweight loss and colonic shortening in DSS mice, and reduced the disease activity index and histopathologic score...
November 26, 2018: Molecular Immunology
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