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Colitis mice

Tao Yu, Ping Wan, Xiao-Dan Zhu, Yu-Ping Ren, Chong Wang, Run-Wei Yan, Yuan Guo, Ai-Ping Bai
NADPH oxidases (NOX) act to generate reactive oxygen species (ROS) and exhibit microbicidal bioactivity, whereas their roles in mediating immune responses of inflammation in intestine remain to be further elucidated. The study was performed to explore the effects of NOX activity on regulation of macrophage functions. Macrophage responses were induced by lipopolysaccharides (LPS) in RAW 264.7 cells (in vitro) or dextran sulfate sodium (DSS) in BALB/c mice (in vivo) respectively. LPS induced NOX2 expression and initiated NOX activities in RAW 264...
October 12, 2018: Biochemical Pharmacology
Robyn Laura Kosinsky, Robert Lorenz Chua, Martin Qui, Dominik Saul, Dawid Mehlich, Philipp Ströbel, Hans-Ulrich Schildhaus, Florian Wegwitz, William A Faubion, Steven A Johnsen
Background and Aims: Inflammatory bowel diseases are linked to an increased risk of developing colorectal cancer (CRC). Previous studies suggested that the H2B ubiquitin ligase RING finger protein-20 (RNF20) inhibited inflammatory signaling mediated by the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). However, the role of RNF40, the obligate heterodimeric partner of RNF20, in the context of inflammation and CRC has not been addressed. Here, we examined the effect of RNF40 loss on CRC cells in vitro and on inflammation and inflammatory signaling in vitro and in vivo...
October 13, 2018: Journal of Crohn's & Colitis
Min Seo Kim, Ji Yeon Kim
This study assessed the anti-inflammatory effect of ginger extract on colitis by 5% dextran sulfate sodium (DSS) in BALB/c mice. The mice were administered either distilled water or three doses of ginger extracts for 21 days. We evaluated the change in clinical and histopathological signs and cytokine and gene expression levels. Contrary to the DSS group, the ginger groups increased body weight and inhibited shortening of the colon. DAI values and colon injury in the ginger groups were lower than that in the DSS group...
October 2018: Food Science and Biotechnology
Ho Pan Sham, Mark Bazett, Momir Bosiljcic, Hyungjun Yang, Beryl Luk, Hong T Law, Vijay Morampudi, Hong B Yu, Jim Pankovich, Simon Sutcliffe, Brian Bressler, John K Marshall, Richard N Fedorak, Jenny Chen, Michelle Jones, Hal Gunn, Shirin Kalyan, Bruce A Vallance
Background: Current ulcerative colitis (UC) treatments are focused on symptom management primarily via immune suppression. Despite the current arsenal of immunosuppressant treatments, the majority of patients with UC still experience disease progression. Importantly, aggressive long-term inhibition of immune function comes with consequent risk, such as serious infections and malignancy. There is thus a recognized need for new, safe and effective treatment strategies for people living with UC that work upstream of managing the symptoms of the disease...
2018: Frontiers in Immunology
Bastian Schirmer, Luisa Bringmann, Roland Seifert, Detlef Neumann
Our previous in vitro studies revealed that histamine via histamine the H4 -receptors (H4 R), as compared to other stimuli, such as eotaxin or formylpeptides, rather partially activates eosinophilic granulocytes (eosinophils). In order to evaluate the H4 R-mediated activation of eosinophils in vivo , we employed dextran sodium sulfate (DSS)-induced colitis in mice, closely resembling human ulcerative colitis (UC), which is largely characterized by a local eosinophilic infiltration of the colon. IL-5-deficient BALB/c mice served as a model with reduced endogenous numbers of eosinophils, in which wild-type (H4 R+/+ ) or H4 R-deficient (H4 R-/- ) eosinophils were adoptively transferred during the course of DSS-induced colitis...
2018: Frontiers in Immunology
Xiaofeng Chen, Xiaofei He, Shijian Luo, Yukun Feng, Fengyin Liang, Taotao Shi, Ruxun Huang, Zhong Pei, Zhendong Li
Cerebral cortical microinfarct (CMI) is common in patients with dementia and cognitive decline. Emerging studies reported that intestinal dysfunction influenced the outcome of ischemic stroke and that vagus nerve stimulation (VNS) protected against ischemic stroke. However, the effects of intestinal dysfunction and VNS on CMI are not clear. Therefore, we examined the influence of colitis and VNS on CMI and the mechanisms of VNS attenuating CMI in mice with colitis. CMI was induced using a two-photon laser. Colitis was induced using oral dextran sodium sulfate (DSS)...
2018: Frontiers in Neurology
Yuzhe Huang, Jian Guo, Shuangying Gui
The current treatments used in inflammatory bowel disease (IBD) therapy have significant side effects. Thus, it is very necessary to position and release TNF-α siRNA in inflamed tissues. Here, we demonstrate that poly(lactic-co-glycolic acid) can pack TNF-α siRNA efficiently into nanoparticles (NPs), and galactosylated chitosan (GC) can be grafted onto the NP surface, improving the RAW 264.7 macrophage-targeting kinetics of the NPs in vitro. Next, we orally administered GC-modified NPs loaded with TNF-α siRNA to C57BL/6 mice treated with 3% dextran sodium sulfate (DSS) to investigate their use in the treatment of colitis...
October 10, 2018: European Journal of Pharmaceutical Sciences
Yilang Tang, Sonja Reissig, Elke Glasmacher, Tommy Regen, Florian Wanke, Alexei Nikolaev, Katharina Gerlach, Vanessa Popp, Khalad Karram, Massimo C Fantini, Jörn M Schattenberg, Peter R Galle, Markus F Neurath, Benno Weigmann, Florian C Kurschus, Nadine Hövelmeyer, Ari Waisman
BACKGROUND & AIMS: The CYLD lysine 63 deubiquitinase gene (CYLD) encodes tumor suppressor protein that is mutated in familial cylindromatosis, and variants have been associated with Crohn's disease (CD). Splice forms of CYLD that lack exons 7 and 8 regulate transcription factors and functions of immune cells. We examined expression of splice forms of CYLD in colon tissues from patients with CD and their effects in mice. METHODS: We performed immunohistochemical analyses of colon tissues from untreated patients with CD and patients without inflammatory bowel diseases (controls)...
October 10, 2018: Gastroenterology
Shuai Wang, Yanke Lin, Xue Yuan, Feng Li, Lianxia Guo, Baojian Wu
The roles of Rev-erbα and circadian clock in colonic inflammation remain unclarified. Here we show colon clock genes (including Rev-erbα) are dysregulated in mice with DSS-induced colitis. In turn, disruption of the circadian clock exacerbates experimental colitis. Rev-erbα-deficient mice are more sensitive to DSS-induced colitis, supporting a critical role of Rev-erbα in disease development. Further, Rev-erbα ablation causes activation of Nlrp3 inflammasome in mice. Cell-based experiments reveal Rev-erbα inactivates Nlrp3 inflammasome mainly at the priming stage...
October 12, 2018: Nature Communications
A Hünerwadel, S Fagagnini, G Rogler, C Lutz, S U Jaeger, C Mamie, B Weder, P A Ruiz, M Hausmann
Intestinal fibrosis is thought to be a consequence of excessive tissue repair, and constitutes a common problem in patients with Crohn's disease (CD). While fibrosis seems to require inflammation as a prerequisite it is unclear whether the severity or persistence of inflammation influences the degree of fibrosis. Our aim was to investigate the role of sustained inflammation in fibrogenesis. For the initiation of fibrosis in vivo the models of Il10-/- spontaneous colitis, dextran sodium sulfate (DSS)-induced chronic colitis and heterotopic transplantation were used...
October 12, 2018: Scientific Reports
Ting-Jia Fan, Sandrine Y Tchaptchet, Diana Arsene, Yoshiyuki Mishima, Bo Liu, R Balfour Sartor, Ian M Carroll, Edward A Miao, Anthony A Fodor, Jonathan J Hansen
Background: Human and mouse studies implicate the inflammasome in the pathogenesis of inflammatory bowel diseases, though the effects in mice are variable. The noncanonical inflammasome activator caspase-11 (Casp11) reportedly attenuates acute dextran sodium sulfate (DSS) colitis in mice. However, the effects of Casp11 on chronic experimental colitis and factors that influence the impact of Casp11 on acute DSS colitis are unknown. Methods: We studied the role of Casp11 in Il10-/- mice and acute and chronic DSS colitis mouse models...
October 12, 2018: Inflammatory Bowel Diseases
Chun-Sai-Er Wang, Wen-Bin Li, Hong-Ying Wang, Yi-Ming Ma, Xin-Hua Zhao, Hong Yang, Jia-Ming Qian, Jing-Nan Li
AIM: To investigate the effects of VSL#3 on tumor formation, and fecal and intestinal mucosal microbiota in azoxymethane/dextran sulfate sodium (AOM/DSS) induced mice model. METHODS: C57BL/6 mice were administered AOM/DSS to develop the ulcerative colitis (UC) carcinogenesis model. Mice were treated with 5-ASA (75 mg/kg/d), VSL#3 (1.5 × 109 CFU/d), or 5-ASA combined with VSL#3 by gavage from the day of AOM injection for three months (five days/week). The tumor load was compared in each group, and tumor necrosis factor (TNF-α) and interleukin (IL)-6 levels were evaluated in colon tissue...
October 7, 2018: World Journal of Gastroenterology: WJG
Awad Mahalhal, Jonathan M Williams, Sophie Johnson, Nicholas Ellaby, Carrie A Duckworth, Michael D Burkitt, Xuan Liu, Georgina L Hold, Barry J Campbell, D Mark Pritchard, Chris S Probert
Inflammatory bowel disease (IBD) is associated with anaemia and oral iron replacement to correct this can be problematic, intensifying inflammation and tissue damage. The intestinal microbiota also plays a key role in the pathogenesis of IBD, and iron supplementation likely influences gut bacterial diversity in patients with IBD. Here, we assessed the impact of dietary iron, using chow diets containing either 100, 200 or 400 ppm, fed ad libitum to adult female C57BL/6 mice in the presence or absence of colitis induced using dextran sulfate sodium (DSS), on (i) clinical and histological severity of acute DSS-induced colitis, and (ii) faecal microbial diversity, as assessed by sequencing the V4 region of 16S rRNA...
2018: PloS One
Yuta Kinoshita, Seiya Arita, Haruka Murazoe, Kazuo Kitamura, Shinya Ashizuka, Kyoko Inagaki-Ohara
Adrenomedullin (AM) exerts a potent anti-inflammatory effect. Intrarectal or consecutive intravenous administrations of AM reduce pathological manifestations in rodent colitis models. However, in clinical applications, a safer administration route that provides stronger alleviation of patient burden is preferred. We investigated whether subcutaneously administered AM is effective against dextran sulfate sodium (DSS)-induced colitis. C57BL/6J mice were administered 1% DSS in drinking water and received AM at 8, 40 or 80 nmol/kg subcutaneously once a day for 7 consecutive days...
October 10, 2018: Human Cell
Alexandre S Hilel, Bruna Gysemans, Maria E M Lisbôa, Ana C Heymanns, Viviane Freiberger, Letícia Ventura, Rachel F Magnago, Clarissa M Comim, Verônica Horewics, Daniel F Martins, Franciane Bobinski, Anna P Piovezan
Citrus species are widely related to antihyperalgesic and anti-inflammatory effects. The aim of this study was to investigate if treatment with ethanolic extract from peels of mature Citrus reticulata Blanco causes antihyperalgesic effects on the referred mechanical hyperalgesia in a model of dextran sulphate of sodium (DSS)-induced colitis in mice, as well as the possible oxidative damage in different regions of the brain induced by its inflammatory reaction. Antihyperalgesia (30 to 300 mg/kg) was investigated by behavioral response (frequency of response to von Frey filament stimulation) in Swiss mice, while damage to central nervous system was investigated through techniques that evaluated oxidative stress using male black C57 BL6 mice (n=8)...
July 2018: Anais da Academia Brasileira de Ciências
Jose J Limon, Kathleen M Kershaw, David M Underhill
PURPOSE OF REVIEW: The intestinal microbiota plays a central role in inflammatory diseases of the gut. Although most investigations regarding how the mucosal immune system interacts with the microbiota have focused on bacteria, recent studies are elucidating the additional role of commensal fungi in health and disease in the gut. RECENT FINDINGS: New technical approaches are defining the makeup of the fungal communities in the intestines of humans and mice. The reported composition of these communities is influenced by the approaches used to define the fungi...
November 2018: Current Opinion in Gastroenterology
Kenjiro Matsumoto, Shinichi Kato
The transient receptor potential vanilloid 4 (TRPV4) is a nonselective cation channel involved in physical sensing in various tissue types. The present study aimed to elucidate the function and expression of TRPV4 in colonic vascular endothelial cells during dextran sulphate sodium (DSS)-induced colitis. The role of TRPV4 in the progression of colonic inflammation was examined in the 2% DSS-induced murine colitis model using immunohistochemical analysis, Western blotting, and Evans blue dye extrusion assay...
2018: Nihon Yakurigaku Zasshi. Folia Pharmacologica Japonica
Tien-Jen Lin, Shu-Yi Yin, Pei-Wen Hsiao, Ning-Sun Yang, I-Jen Wang
The incidence of inflammatory bowel disease (IBD) has markedly increased. Our research findings during the past showed that medicinal plant extracts and the derived phytochemical components from Wedelia chinensis (WC) can have strong anti-colitis activities. Here, we further identified the key component phytochemicals from active fractions of different WC preparations (WCHA) that are responsible for the protective effect of WCHA in colitis mice. Of the 3 major compounds (wedelolactone, luteolin and apigenin) in this fraction, luteolin had the highest anti-inflammatory effect in vivo...
October 8, 2018: Scientific Reports
Wei Liu, Yu Zhang, Bin Qiu, Shoujin Fan, Hanfeng Ding, Zhenhua Liu
A plethora of evidence highlights that the dysbiosis of gut microbiota is a critical factor for inflammatory bowel disease (IBD). Both in vivo and in vitro studies have demonstrated that quinoa possesses potential prebiotic effects. The present study aims to examine the potential in using quinoa to ameliorate the dysbiosis and colitis induced by dextran sodium sulfate (DSS). A total of 40 C57BL/6 mice were fed either an AIN-93M diet or a quinoa-based diet, separately. Colitis was induced for 10 animals/dietary group with a 5-days exposure to 2...
October 8, 2018: Scientific Reports
Yasuharu Nagahama, Mayuko Shimoda, Guoliang Mao, Shailendra Kumar Singh, Yuuki Kozakai, Xin Sun, Daisuke Motooka, Shota Nakamura, Hiroki Tanaka, Takashi Satoh, Kazuhiko Maeda, Shizuo Akira
Damage to intestinal epithelial cell (IEC) layers during intestinal inflammation is associated with inflammatory bowel disease. Here we show that the endoribonuclease Regnase-1 controls colon epithelial regeneration by regulating protein kinase mTOR (the mechanistic target of rapamycin kinase) and purine metabolism. During dextran sulfate sodium-induced intestinal epithelial injury and acute colitis, Regnase-1∆IEC mice, which lack Regnase-1 specifically in the intestinal epithelium, were resistant to body weight loss, maintained an intact intestinal barrier, and showed increased cell proliferation and decreased epithelial apoptosis...
October 8, 2018: Proceedings of the National Academy of Sciences of the United States of America
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