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José Miguel Quesada, Inés Aguilar, Jesús de la Torre, Regina-Michaela Wittich, Pieter van Dillewijn
Here, we report the genome sequences of one Achromobacter and four Pseudomonas strains isolated from sediments of the River Elbe which are highly tolerant toward the xenobiotic target compound diclofenac, a nonsteroidal anti-inflammatory drug (NSAID) and emerging contaminant.
August 2018: Microbiology resource announcements
U Brockmann, D Topcu, M Schütt, W Leujak
The eutrophication status of the German Bight (North Sea) has been assessed the third time since 1998 according to the OSPAR-Comprehensive Procedure between 2006 and 2014. Since the 1980s nutrient discharges and atmospheric nitrogen deposition had declined significantly but chlorophyll a and nutrient concentrations remained above assessment levels inshore and in inner coastal waters, reflecting continuing eutrophication. Recently local river discharges stagnated or increased again and total nitrogen remained above a reduction target of 200 μM...
November 2018: Marine Pollution Bulletin
Karen L Elbing, Roger Brent
We describe the procedure for inoculating overnight (starter) cultures of E. coli from a single colony, along with considerations for growing larger cultures. We also include two methods for monitoring the number of cells per unit volume (density) of liquid cultures using a spectrophotometer and a hemacytometer or "count slide." © 2018 by John Wiley & Sons, Inc.
November 9, 2018: Current Protocols in Molecular Biology
Karen L Elbing, Roger Brent
In this article, we provide information about culture media, including minimal liquid media, rich liquid media, solid media, top agar, and stab agar. We also provide descriptions and useful information about tools used with growth media such as inoculating loops, sterile toothpicks, and spreaders. © 2018 by John Wiley & Sons, Inc.
November 9, 2018: Current Protocols in Molecular Biology
A M Bierschenk, M Mueller, J Pander, J Geist
Intensification of catchment land-use and the corresponding habitat degradation pose a threat to freshwater biodiversity and ecosystem health, yet few studies comprehensively quantified the effects of specific land-use variables on fish communities for different catchments within the same climatic region. Herein, we investigated the influence of catchment land use on fish community composition in the headwater areas of the European main river systems Elbe, Danube and Main/Rhine. The analyses comprising 289 streams and rivers in Bavaria, southern Germany, revealed that the influence of urbanization (e...
October 16, 2018: Science of the Total Environment
Louise Bundgaard, Allan Stensballe, Kirstine Juul Elbæk, Lise Charlotte Berg
BACKGROUND: Stem cells have great potential for tissue regeneration, but before stem cell populations can be used in the clinic, it is crucial that the stem cells have been definitely characterized by a set of specific markers. Although there have been attempts to identify a set of immunophenotypic markers to characterize equine mesenchymal stromal cells (MSCs), immunophenotyping of equine MSCs is still challenging due to the limited availability of suitable antibodies of high quality and consistent performance across different laboratories...
October 25, 2018: Stem Cell Research & Therapy
Virginie Dubes, David Benoist, François Roubertie, Stephen H Gilbert, Marion Constantin, Sabine Charron, Delphine Elbes, Delphine Vieillot, Bruno Quesson, Hubert Cochet, Michel Haïssaguerre, Caroline Rooryck, Pierre Bordachar, Jean-Benoit Thambo, Olivier Bernus
BACKGROUND: Ventricular arrhythmias are frequent in patients with repaired tetralogy of Fallot (rTOF), but their origin and underlying mechanisms remain unclear. In this study, the involvement of left ventricular (LV) electrical and structural remodeling was assessed in an animal model mimicking rTOF sequelae. METHODS: Piglets underwent a tetralogy of Fallot repair-like surgery (n=6) or were sham operated (Sham, n=5). Twenty-three weeks post-surgery, cardiac function was assessed in vivo by magnetic resonance imaging...
October 2018: Circulation. Arrhythmia and Electrophysiology
Julie Elbæk Pedersen, Kajsa Ugelvig Petersen, Niels Erik Ebbehøj, Jens Peter Bonde, Johnni Hansen
OBJECTIVE: Firefighters are exposed to numerous respiratory hazards, but large studies on the risk of pulmonary disease are scarce. The objective of this study was to examine incidence of asthma and chronic obstructive pulmonary disease (COPD) in a nationwide cohort of Danish firefighters. METHODS: We used individual historical employment records on 11 968 Danish male firefighters primarily supplied by trade unions and fire agencies. Furthermore, we used the Supplementary Pension Fund Register to form an occupational reference group consisting of military employees...
October 15, 2018: Occupational and Environmental Medicine
B Tutun, H Elbe, N Vardi, H Parlakpinar, A Polat, M Gunaltili, M M Guclu, E N Yasar
Hyperglycemia increases reactive oxygen species (ROS) and the resulting oxidative stress contributes to the development of diabetic complications. Dexpanthenol (Dxp) is the biological active form of pantothenic acid. We investigated whether Dxp administration could decrease oxidative stress as a way to treat renal complications of diabetes mellitus (DM). Thirty-two male Wistar albino rats were divided into four groups: control, Dxp, DM and DM + Dxp. Experimental diabetes was induced by a single dose of streptozotocin (STZ)...
October 15, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
Thomas Kurz, Jurjen Pieter Couperus, Jakob Matthias Krämer, Hao Ding, Stephan Kuschel, Alexander Köhler, Omid Zarini, Dominik Hollatz, David Schinkel, Richard D'Arcy, Jan-Patrick Schwinkendorf, Jens Osterhoff, Arie Irman, Ulrich Schramm, Stefan Karsch
We revise the calibration of scintillating screens commonly used to detect relativistic electron beams with low average current, e.g., from laser-plasma accelerators, based on new and expanded measurements that include higher charge density and different types of screens than previous work [Buck et al. , Rev. Sci. Instrum. 81 , 033301 (2010)]. Electron peak charge densities up to 10 nC/mm2 were provided by focused picosecond-long electron beams delivered by the Electron Linac for beams with high Brilliance and low Emittance (ELBE) at the Helmholtz-Zentrum Dresden-Rossendorf...
September 2018: Review of Scientific Instruments
Tanja Wagner, Maria Gschwandtner, Agata Strajeriu, Adelheid Elbe-Bürger, Johannes Grillari, Regina Grillari-Voglauer, Georg Greiner, Bahar Golabi, Erwin Tschachler, Michael Mildner
The advent of organotypic skin models advanced the understanding of complex mechanisms of keratinocyte differentiation. However, these models are limited by both availability of primary keratinocytes and donor variability. Keratinocytes derived from cultured hair follicles and interfollicular epidermis were immortalized by ectopic expression of SV40 and hTERT. The generated keratinocyte cell lines differentiated into stratified epidermis with well-defined stratum granulosum and stratum corneum in organotypic human skin models...
September 7, 2018: Scientific Reports
Kerstin B Ksionzek, Jing Zhang, Kai-Uwe Ludwichowski, Dorothee Wilhelms-Dick, Scarlett Trimborn, Thomas Jendrossek, Gerhard Kattner, Boris P Koch
Dissolved organic matter (DOM) is ubiquitous in natural waters and plays a central role in the biogeochemistry in riverine, estuarine and marine environments. This study quantifies and characterizes solid-phase extractable DOM and trace element complexation at different salinities in the Weser and Elbe River, northern Germany, and the North Sea. Dissolved organic carbon (DOC), total dissolved nitrogen (TDN), Co and Cu concentrations were analyzed in original water samples. Solid-phase extracted (SPE) water samples were analyzed for DOC (DOCSPE), dissolved organic nitrogen (DONSPE), sulfur (DOSSPE) and trace metal (51V, 52Cr, 59Co, 60Ni, 63Cu, 75As) concentrations...
2018: PloS One
Mette Vestergaard Elbæk, Anders Lindholm Sørensen, Hans Carl Hasselbalch
Patients with chronic myelomonocytic leukemia (CMML) have monocytosis and likely a state of chronic inflammation. Both have been associated with an increased risk of atherosclerosis. The aim of the study was to test the hypothesis that CMML patients are at increased risk of developing cardiovascular disease (CVD) due to persistent monocytosis and sustained chronic inflammation. In a retrospective cohort study, we assessed hazards for cardiovascular events after diagnosis in 112 CMML patients and 231 chronic lymphocytic leukemia (CLL) patients...
September 4, 2018: Annals of Hematology
M Laabs, N Neumann, B Green, N Awari, J Deinert, S Kovalev, D Plettemeier, M Gensch
The layout of an integrated millimetre-scale on-chip THz spectrometer is presented and its peformance demonstrated. The device is based on eight Schottky-diode detectors which are combined with narrowband THz antennas, thereby enabling the simultaneous detection of eight frequencies in the THz range on one chip. The size of the active detector area matches the focal spot size of superradiant THz radiation utilized in bunch compression monitors of modern linear electron accelerators. The 3 dB bandwidth of the on-chip Schottky-diode detectors is less than 10% of the center frequency and allows pulse-resolved detection at up to 5 GHz repetition rates...
September 1, 2018: Journal of Synchrotron Radiation
Apostolos Analatos, Mats Lindblad, Ioannis Rouvelas, Peter Elbe, Lars Lundell, Magnus Nilsson, Andrianos Tsekrekos, Jon A Tsai
BACKGROUND: Primary antireflux surgery has high success rates but 5 to 20% of patients undergoing antireflux operations can experience recurrent reflux and dysphagia, requiring reoperation. Different surgical approaches after failed fundoplication have been described in the literature. The aim of this study was to evaluate resection of the gastroesophageal junction with jejunal interposition (Merendino procedure) as a rescue procedure after failed fundoplication. METHODS: All patients who underwent a Merendino procedure at the Karolinska University Hospital between 2004 and 2012 after a failed antireflux fundoplication were identified...
August 30, 2018: BMC Surgery
Nora Claire Niehus, Sabine Schäfer, Christel Möhlenkamp, Gesine Witt
Background: Chemical quality of sediment and suspended particulate matter (SPM) is usually assessed by total chemical concentrations ( C total ). However, the freely dissolved concentration ( C free ) is the ecologically more relevant parameter for bioavailability, diffusion and bioaccumulation. In recent studies, equilibrium sampling has been applied to determine C free of hydrophobic organic contaminants (HOCs) in the sediment pore water, whereas such data are missing for SPM. We applied solid-phase micro-extraction to measure and compare C free of PAHs and PCBs in pore water of sediments and SPM sampled along the German part of the river Elbe...
2018: Environmental Sciences Europe
Viktoriia Burkina, Galia Zamaratskaia, Sidika Sakalli, Pham Thai Giang, Vit Kodes, Roman Grabic, Josef Velisek, Jan Turek, Jitka Kolarova, Vladimir Zlabek, Tomas Randak
Monitoring the contamination level in aquatic environments and assessing the impact on aquatic life occurs throughout the world. In the present study, an approach based on a combination of biomarkers and the distribution of various industrial and municipal pollutants was used to investigate the effect of aquatic environmental contamination on fish. Monitoring was performed in ten rivers in the Czech Republic (Berounka, Dyje, Elbe, Lužnice, Odra, Ohře, Otava, Sázava, Svratka, and Vltava rivers, with one or two locations in each river) at the same sites that were regularly monitored within the Czech National Monitoring Program in 2007-2011...
August 8, 2018: Ecotoxicology and Environmental Safety
Thomas Bechter, Kathrin Baumann, Sebastian Birk, Fabian Bolik, Wolfram Graf, Florian Pletterbauer
Large rivers cover and function over large spatial extents. Accordingly, the detailed assessment of their morphology is complex. Here, a methodology is presented to assess large rivers' morphology (LaRiMo) based on free datasets of geographic information systems. This approach could help to achieve a comparable, transboundary assessment of large river morphology to support the implementation of the European Water Framework Directive. The analyses are based on seven parameters describing processes and characteristics related to large river morphology...
July 1, 2018: Science of the Total Environment
Bernhard Kratzer, Cordula Köhler, Sandra Hofer, Ursula Smole, Doris Trapin, Jagoba Iturri, Dietmar Pum, Philip Kienzl, Adelheid Elbe-Bürger, Pia Gattinger, Irene Mittermann, Birgit Linhart, Gabriele Gadermaier, Beatrice Jahn-Schmid, Alina Neunkirchner, Rudolf Valenta, Winfried F Pickl
BACKGROUND: In high-risk populations, allergen-specific prophylaxis could protect from sensitization and subsequent development of allergic disease. However, such treatment might itself induce sensitization and allergies, thus requiring hypo-allergenic vaccine formulations. We here characterized the preventive potential of virus-like nanoparticles (VNP) expressing surface exposed or shielded allergens. METHODS: Full-length major mugwort pollen allergen Art v 1 was selectively targeted either to the surface or to the inner side of the lipid bilayer envelope of VNP...
July 23, 2018: Allergy
Peter Elsborg, Anne-Marie Elbe
OBJECTIVE: The present study investigated the influence of exercise-specific motivation and volition on weight loss maintenance following an intensive lifestyle intervention (ILI). METHOD: The study participants were 164 individuals with obesity (body mass index [BMI] > 30 kg/m2) that participated in an ILI. Participants' exercise-specific volition and motivation were assessed before and after the intervention as well as 3 and 12 months after completion. Volition was measured with the Volition in Exercise Questionnaire and motivation was measured with the context translated Sport Motivation Scale...
August 2018: Health Psychology: Official Journal of the Division of Health Psychology, American Psychological Association
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