keyword
https://read.qxmd.com/read/36920018/a-rationally-designed-complex-replenishes-the-transferrin-iron-pool-directly-and-with-high-specificity
#21
JOURNAL ARTICLE
Artur Sargun, Allison L Fisher, Anna S Wolock, Sydney Phillips, Mozhdeh Sojoodi, Soumya Khanna, Jodie L Babitt, Eric M Gale
Many forms of anemia are caused or complicated by pathologic restriction of iron (Fe). Chronic inflammation and certain genetic mutations decrease the activity of ferroportin, the only Fe-exporter protein, so that endogenously recycled or nutritionally absorbed Fe cannot be exported to the extracellular Fe carrier protein transferrin for delivery to the bone marrow. Diminished ferroportin activity renders anemia correction challenging as Fe administered intravenously or through nutritional supplementation is trafficked through the ferroportin-transferrin axis...
March 15, 2023: Journal of the American Chemical Society
https://read.qxmd.com/read/36808999/standardized-preclinical-in-vitro-blood-brain-barrier-mouse-assay-validates-endocytosis-dependent-antibody-transcytosis-using-transferrin-receptor-mediated-pathways
#22
JOURNAL ARTICLE
Jamie I Morrison, Alex Petrovic, Nicole G Metzendorf, Fadi Rofo, Canan U Yilmaz, Sofia Stenler, Hanna Laudon, Greta Hultqvist
The presence of the blood-brain barrier (BBB) creates a nigh-on impenetrable obstacle for large macromolecular therapeutics that need to be delivered to the brain milieu to treat neurological disorders. To overcome this, one of the strategies used is to bypass the barrier with what is referred to as a "Trojan Horse" strategy, where therapeutics are designed to use endogenous receptor-mediated pathways to piggyback their way through the BBB. Even though in vivo methodologies are commonly used to test the efficacy of BBB-penetrating biologics, comparable in vitro BBB models are in high demand, as they benefit from being an isolated cellular system devoid of physiological factors that can on occasion mask the processes behind BBB transport via transcytosis...
February 21, 2023: Molecular Pharmaceutics
https://read.qxmd.com/read/36779337/eukaryotic-clathrin-adapter-protein-and-mediator-of-cholesterol-homeostasis-picalm-affects-trafficking-to-the-chlamydial-inclusion
#23
JOURNAL ARTICLE
Lisa M Jorgenson, Lindsey Knight, Ray E Widner, Elizabeth A Rucks
The obligate intracellular pathogen Chlamydia trachomatis has unique metabolic requirements as it proceeds through its biphasic developmental cycle from within the inclusion within the host cell. In our previous study, we identified a host protein, PICALM, which localizes to the chlamydial inclusion. PICALM functions in many host pathways including the recycling of receptors, specific SNARE proteins, and molecules like transferrin, and maintaining cholesterol homeostasis. Hence, we hypothesized that PICALM functions to maintain the cholesterol content and to moderate trafficking from the endosomal recycling pathway to the inclusion, which controls chlamydial access to this pathway...
February 13, 2023: Molecular and Cellular Biology
https://read.qxmd.com/read/36752571/atm-orchestrates-ferritinophagy-and-ferroptosis-by-phosphorylating-ncoa4
#24
JOURNAL ARTICLE
Hao Wu, Qian Liu, Xinyi Shan, Weihua Gao, Quan Chen
Ferroptosis is a newly characterized form of programmed cell death, which is driven by the lethal accumulation of lipid peroxides catalyzed by the intracellular bioactive iron. Targeted induction of ferroptotic cell death holds great promise for therapeutic design against other therapy-resistant cancers. To date, multiple post-translational modifications have been elucidated to impinge on the ferroptotic sensitivity. Here we report that the Ser/Thr protein kinase ATM, the major sensor of DNA double-strand break damage, is indispensable for ferroptosis execution...
February 8, 2023: Autophagy
https://read.qxmd.com/read/36638190/elevated-transferrin-receptor-impairs-t-cell-metabolism-and-function-in-systemic-lupus-erythematosus
#25
JOURNAL ARTICLE
Kelsey Voss, Allison E Sewell, Evan S Krystofiak, Katherine N Gibson-Corley, Arissa C Young, Jacob H Basham, Ayaka Sugiura, Emily N Arner, William N Beavers, Dillon E Kunkle, Megan E Dickson, Gabriel A Needle, Eric P Skaar, W Kimryn Rathmell, Michelle J Ormseth, Amy S Major, Jeffrey C Rathmell
T cells in systemic lupus erythematosus (SLE) exhibit multiple metabolic abnormalities. Excess iron can impair mitochondria and may contribute to SLE. To gain insights into this potential role of iron in SLE, we performed a CRISPR screen of iron handling genes on T cells. Transferrin receptor (CD71) was identified as differentially critical for TH 1 and inhibitory for induced regulatory T cells (iTregs ). Activated T cells induced CD71 and iron uptake, which was exaggerated in SLE-prone T cells. Cell surface CD71 was enhanced in SLE-prone T cells by increased endosomal recycling...
January 13, 2023: Science Immunology
https://read.qxmd.com/read/36631201/regulation-of-transferrin-receptor-trafficking-by-optineurin-and-its-disease-associated-mutants
#26
JOURNAL ARTICLE
Shivranjani C Moharir, Kapil Sirohi, Ghanshyam Swarup
Transferrin receptor (TFRC) is a transmembrane protein that plays a crucial role in mediating homeostasis of iron in the cell. The binding of transferrin (that is bound to iron) to TFRC at the cell membrane generally starts endocytosis of TFRC-transferrin complex, which leads to formation of vesicles that are positive for TFRC. These vesicles travel to the early endosomes and later to the endocytic recycling compartment. Release of iron occurs in the early endosomes because of acidic pH. Major fraction of the transferrin and TFRC is transported back to the cell membrane; however, a minor fraction of it is transported to lysosomes through the process of autophagy...
2023: Progress in Molecular Biology and Translational Science
https://read.qxmd.com/read/36446521/loss-of-ndr1-2-kinases-impairs-endomembrane-trafficking-and-autophagy-leading-to-neurodegeneration
#27
JOURNAL ARTICLE
Flavia Roşianu, Simeon R Mihaylov, Noreen Eder, Antonie Martiniuc, Suzanne Claxton, Helen R Flynn, Shamsinar Jalal, Marie-Charlotte Domart, Lucy Collinson, Mark Skehel, Ambrosius P Snijders, Matthias Krause, Sharon A Tooze, Sila K Ultanir
Autophagy is essential for neuronal development and its deregulation contributes to neurodegenerative diseases. NDR1 and NDR2 are highly conserved kinases, implicated in neuronal development, mitochondrial health and autophagy, but how they affect mammalian brain development in vivo is not known. Using single and double Ndr1/2 knockout mouse models, we show that only dual loss of Ndr1/2 in neurons causes neurodegeneration. This phenotype was present when NDR kinases were deleted both during embryonic development, as well as in adult mice...
February 2023: Life Science Alliance
https://read.qxmd.com/read/36314015/host-and-microbiota-derived-extracellular-vesicles-crucial-players-in-iron-homeostasis
#28
REVIEW
Yasmeen Daou, Marion Falabrègue, Charareh Pourzand, Carole Peyssonnaux, Marvin Edeas
Iron is a double-edged sword. It is vital for all that's living, yet its deficiency or overload can be fatal. In humans, iron homeostasis is tightly regulated at both cellular and systemic levels. Extracellular vesicles (EVs), now known as major players in cellular communication, potentially play an important role in regulating iron metabolism. The gut microbiota was also recently reported to impact the iron metabolism process and indirectly participate in regulating iron homeostasis, yet there is no proof of whether or not microbiota-derived EVs interfere in this relationship...
2022: Frontiers in Medicine
https://read.qxmd.com/read/36295822/associated-effect-of-slc40a1-and-tmprss6-polymorphisms-on-iron-overload
#29
JOURNAL ARTICLE
Lorena Duca, Francesca Granata, Elena Di Pierro, Valentina Brancaleoni, Giovanna Graziadei, Isabella Nava
Mutations in the ferroportin (FPN) gene SLC40A1 alter iron recycling and cause disturbances in iron homeostasis. The variants of TMPRSS6 contribute to the development of iron deficiencies. In this study, we determined the role of FPN and TMPRSS6 gene polymorphisms in the modulation of iron homeostasis based on biochemical parameters. PCR analysis and sequencing were performed to determine the single nucleotide polymorphisms (SNPs) SLC40A1 c.44-24G>C (rs1439816), SLC40A1 c.663T>C (rs2304704), and TMPRSS6 c...
September 29, 2022: Metabolites
https://read.qxmd.com/read/36123369/inhibitors-of-rna-and-protein-synthesis-cause-glut4-translocation-and-increase-glucose-uptake-in-adipocytes
#30
JOURNAL ARTICLE
A B Meriin, N Zaarur, J S Bogan, K V Kandror
Insulin stimulates glucose uptake in adipocytes by triggering translocation of glucose transporter 4-containg vesicles to the plasma membrane. Under basal conditions, these vesicles (IRVs for insulin-responsive vesicles) are retained inside the cell via a "static" or "dynamic" mechanism. We have found that inhibitors of RNA and protein synthesis, actinomycin D and emetine, stimulate Glut4 translocation and glucose uptake in adipocytes without engaging conventional signaling proteins, such as Akt, TBC1D4, or TUG...
September 19, 2022: Scientific Reports
https://read.qxmd.com/read/36002835/rapid-increase-in-transferrin-receptor-recycling-promotes-adhesion-during-t-cell-activation
#31
JOURNAL ARTICLE
Pascal Rossatti, Gregory M I Redpath, Luca Ziegler, Guerric P B Samson, Camille D Clamagirand, Daniel F Legler, Jérémie Rossy
BACKGROUND: T cell activation leads to increased expression of the receptor for the iron transporter transferrin (TfR) to provide iron required for the cell differentiation and clonal expansion that takes place during the days after encounter with a cognate antigen. However, T cells mobilise TfR to their surface within minutes after activation, although the reason and mechanism driving this process remain unclear. RESULTS: Here we show that T cells transiently increase endocytic uptake and recycling of TfR upon activation, thereby boosting their capacity to import iron...
August 24, 2022: BMC Biology
https://read.qxmd.com/read/35833460/basolateral-sorting-of-the-sodium-iodide-symporter-is-mediated-by-adaptor-protein-1-clathrin-adaptor-complexes
#32
JOURNAL ARTICLE
Petrina Koumarianou, Celia Fernández-Méndez, Dánae Fajardo-Delgado, Lidia Mirella Mielu, Pilar Santisteban, Antonio De la Vieja
<b><i>Background:</i></b> The sodium/iodide symporter (NIS) is a transmembrane protein located on the basolateral membrane of thyrocytes. Despite its physiological and clinical relevance, little is known about the mechanisms that mediate NIS subcellular sorting. In the present study, we examined NIS basolateral trafficking <i>in vitro</i> using non-thyroid and thyroid epithelial cells. <b><i>Methods:</i></b> Immunofluorescence and Western blotting were performed to analyze NIS subcellular location and function in cells grown in monolayers under unpolarized and/or polarized conditions...
October 2022: Thyroid: Official Journal of the American Thyroid Association
https://read.qxmd.com/read/35791114/lactoferrin-and-its-nano-formulations-in-rare-eye-diseases
#33
REVIEW
Jiya Singh, Mohita Sharma, Neha Jain, Insha Aftab, Naval Vikram, Tej P Singh, Pradeep Sharma, Sujata Sharma
Lactoferrin (LF) is an iron-binding glycoprotein released from mucous secreting cells and neutrophils. LF can be used in a broad range of eye diseases related to the retina, cornea, and optic nerve. The retina is particularly affected by oxidative stress inside the photoreceptor being constantly exposed to light which induces accumulation of reactive oxygen species (ROS) in the retinal pigmented epithelium (RPE) causing damage to photoreceptor recycling. Retinitis pigmentosa (RP) and macular degeneration are inherited retinopathies that consist of different disease-causing genes, that cause mutations with highly varied clinical consequences...
July 2022: Indian Journal of Ophthalmology
https://read.qxmd.com/read/35770989/endocytosed-hiv-1-envelope-glycoprotein-traffics-to-rab14-late-endosomes-and-lysosomes-to-regulate-surface-levels-in-t-cell-lines
#34
JOURNAL ARTICLE
Huxley K Hoffman, Rebekah S Aguilar, Austin R Clark, Nicholas S Groves, Nairi Pezeshkian, Merissa M Bruns, Schuyler B van Engelenburg
Production of infectious HIV-1 particles requires incorporation of the viral envelope glycoprotein (Env) at the plasma membrane (PM) of infected CD4+ T cells. Env trafficking to the PM exposes viral epitopes that can be exploited by the host immune system; however, HIV-1 can evade this response by endocytosis of excess Env from the PM. The fate of Env after internalization remains unclear, with evidence suggesting several different vesicular trafficking steps may be involved, including recycling pathways. To date, there have been very few studies documenting the trafficking pathways of native Env in infected T cells...
June 30, 2022: Journal of Virology
https://read.qxmd.com/read/35753407/the-small-gtpase-rab10-regulates-endosomal-recycling-of-the-ldl-receptor-and-transferrin-receptor-in-hepatocytes
#35
JOURNAL ARTICLE
Taslima Gani Khan, David Ginsburg, Brian T Emmer
The low-density lipoprotein receptor (LDLR) mediates the hepatic uptake of circulating low-density lipoproteins (LDL), a process that modulates the development of atherosclerotic cardiovascular disease. We recently identified RAB10, encoding a small GTPase, as a positive regulator of LDL uptake in hepatocellular carcinoma cells (HuH7) in a genome-wide CRISPR screen, though the underlying molecular mechanism for this effect was unknown. We now report that RAB10 regulates hepatocyte LDL uptake by promoting the recycling of endocytosed LDLR from RAB11-positive endosomes to the plasma membrane...
June 23, 2022: Journal of Lipid Research
https://read.qxmd.com/read/35743233/dmt1-protects-macrophages-from-salmonella-infection-by-controlling-cellular-iron-turnover-and-lipocalin-2-expression
#36
JOURNAL ARTICLE
Manuel Grander, Alexander Hoffmann, Markus Seifert, Egon Demetz, Philipp Grubwieser, Christa Pfeifhofer-Obermair, David Haschka, Günter Weiss
Macrophages are at the center of innate pathogen control and iron recycling. Divalent metal transporter 1 (DMT1) is essential for the uptake of non-transferrin-bound iron (NTBI) into macrophages and for the transfer of transferrin-bound iron from the endosome to the cytoplasm. As the control of cellular iron trafficking is central for the control of infection with siderophilic pathogens such as Salmonella Typhimurium, a Gram-negative bacterium residing within the phagosome of macrophages, we examined the potential role of DMT1 for infection control...
June 17, 2022: International Journal of Molecular Sciences
https://read.qxmd.com/read/35710628/genome-wide-meta-analysis-of-iron-status-biomarkers-and-the-effect-of-iron-on-all-cause-mortality-in-hunt
#37
JOURNAL ARTICLE
Marta R Moksnes, Sarah E Graham, Kuan-Han Wu, Ailin Falkmo Hansen, Sarah A Gagliano Taliun, Wei Zhou, Ketil Thorstensen, Lars G Fritsche, Dipender Gill, Amy Mason, Francesco Cucca, David Schlessinger, Gonçalo R Abecasis, Stephen Burgess, Bjørn Olav Åsvold, Jonas B Nielsen, Kristian Hveem, Cristen J Willer, Ben M Brumpton
Iron is essential for many biological processes, but iron levels must be tightly regulated to avoid harmful effects of both iron deficiency and overload. Here, we perform genome-wide association studies on four iron-related biomarkers (serum iron, serum ferritin, transferrin saturation, total iron-binding capacity) in the Trøndelag Health Study (HUNT), the Michigan Genomics Initiative (MGI), and the SardiNIA study, followed by their meta-analysis with publicly available summary statistics, analyzing up to 257,953 individuals...
June 16, 2022: Communications Biology
https://read.qxmd.com/read/35708998/on-demand-expression-control-of-endogenous-genes-with-dexcon-dexogron-and-luxon-reveals-differential-dynamics-of-rab11-family-members
#38
JOURNAL ARTICLE
Jakub Gemperle, Thomas S Harrison, Chloe Flett, Antony D Adamson, Patrick T Caswell
CRISPR technology has made generation of gene knock-outs widely achievable in cells. However, once inactivated, their re-activation remains difficult, especially in diploid cells. Here, we present DExCon (<u>D</u>oxycycline-mediated endogenous gene <u>Ex</u>pression <u>Con</u>trol), DExogron (DExCon combined with auxin-mediated targeted protein degradation), and LUXon (light responsive DExCon) approaches which combine one-step CRISPR-Cas9-mediated targeted knockin of fluorescent proteins with an advanced Tet-inducible TRE3GS promoter...
June 16, 2022: ELife
https://read.qxmd.com/read/35618957/the-mutual-crosstalk-between-iron-and-erythropoiesis
#39
REVIEW
Clara Camaschella, Alessia Pagani, Laura Silvestri, Antonella Nai
Iron homeostasis and erythropoiesis are strongly interconnected. On one side iron is essential to terminal erythropoiesis for hemoglobin production, on the other erythropoiesis may increase iron absorption through the production of erythroferrone, the erythroid hormone that suppresses hepcidin expression Also erythropoietin production is modulated by iron through the iron regulatory proteins-iron responsive elements that control the hypoxia inducible factor 2-α. The second transferrin receptor, an iron sensor both in the liver and in erythroid cells modulates erythropoietin sensitivity and is a further link between hepcidin and erythropoiesis...
August 2022: International Journal of Hematology
https://read.qxmd.com/read/35604355/%C3%AE-synuclein-fibrils-explore-actin-mediated-macropinocytosis-for-cellular-entry-into-model-neuroblastoma-neurons
#40
JOURNAL ARTICLE
Pravin Hivare, Joshna Gadhavi, Dhiraj Bhatia, Sharad Gupta
Alpha-Synuclein (α-Syn), an intrinsically disordered protein (IDP), is associated with neurodegenerative disorders, including Parkinson's disease (PD) or other α-synucleinopathies. Recent investigations propose the transmission of α-Syn protein fibrils, in a prion-like manner, by entering proximal cells to seed further fibrillization in PD. Despite the recent advances, the mechanisms by which extracellular protein aggregates internalize into the cells remain poorly understood. Using a simple cell-based model of human neuroblastoma-derived differentiated neurons, we present the cellular internalization of α-Syn PFF to check cellular uptake and recycling kinetics along with the standard endocytic markers Transferrin (Tf) marking clathrin-mediated endocytosis (CME) and Galectin3 (Gal3) marking clathrin-independent endocytosis (CIE)...
May 23, 2022: Traffic
keyword
keyword
166589
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.