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cell implantation

Balaji Ramachandran, Sudip Chakraborty, Ramya Kannan, Madhulika Dixit, Vignesh Muthuvijayan
Hyaluronic acid from metabolically engineered Lactococcus lactis (HAL ) was characterized for its biocompatibility and immobilized on the polyethylene terephthalate (PET) surface. HAL was chemically crosslinked on hydrolyzed PET (hPET) surface to form HAL -coated PET (hPET-HAL ). The unmodified and modified PET were characterized by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), contact angle measurement, thermogravimetric analysis (TGA), universal testing machine (UTM) and assessed for their biocompatibility...
February 15, 2019: Carbohydrate Polymers
Agata Kurowski, Andrei Molotkov, Philippe Soriano
FGF signaling plays important roles in many aspects of mammalian development. Fgfr1-/- and Fgfr1-/- Fgfr2-/- mouse embryos on a 129S4 co-isogenic background fail to survive past the peri-implantation stage, whereas Fgfr2-/- embryos die at midgestation and show defects in limb and placental development. To investigate the basis for the Fgfr1-/- and Fgfr1-/- Fgfr2-/- peri-implantation lethality, we examined the role of FGFR1 and FGFR2 in trophectoderm (TE) development. In vivo, Fgfr1-/- TE cells failed to downregulate CDX2 in the mural compartment and exhibited abnormal apicobasal E-Cadherin polarity...
December 12, 2018: Developmental Biology
Kenichiro Taniguchi, Idse Heemskerk, Deborah L Gumucio
Proper development of the human embryo following its implantation into the uterine wall is critical for the successful continuation of pregnancy. However, the complex cellular and molecular changes that occur during this post-implantation period of human development are not amenable to study in vivo. Recently, several new embryo-like human pluripotent stem cell (hPSC)-based platforms have emerged, which are beginning to illuminate the current black box state of early human post-implantation biology. In this review, we will discuss how these experimental models are carving a way for understanding novel molecular and cellular mechanisms during early human development...
December 14, 2018: Journal of Cell Biology
Susanne Jung, Lauren Bohner, Marcel Hanisch, Johannes Kleinheinz, Sonja Sielker
For the guided regeneration of periimplant hard and soft tissues, human adipose-derived stromal cells (hADSC) seem to be a promising source for mesenchymal stromal cells. For this, the proliferation and differentiation of hADSC were evaluated on titanium and zirconia dental implants with different surface treatments. Results were compared to edaphic cells as human osteoblasts (hOB) and human gingival fibroblasts (HGF). Primary cells were cultured on (1) titanium implants with a polished surface (Ti-PT), (2) sandblasted and acid-etched titanium (Ti-SLA), (3) sandblasted and alkaline etched zirconia (ZrO₂-ZLA) and (4) machined zirconia (ZrO₂-M)...
December 13, 2018: International Journal of Molecular Sciences
Shimao Yang, Yanwei Guo, Wenmei Zhang, Jin Zhang, Yujie Zhang, Peng Xu
Implant bone defects are the most common phenomenon in the processes of bone transplantation. Evidences have identified that fibroblast growth factor-21 (FGF-21) encourages osteogenesis for patients with implant bone defects. The purpose of this study was to investigate the role of FGF-21 and its potential mechanism in bone mesenchymal stem cells (BMSCs). RT-PCR, Western blotting, flow cytometry, immunofluorescence and immunohistochemistry assays were performed to analyze the role of FGF-21 and intracellular signaling pathways involved in BMSCs...
January 2019: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Qing Ban, Wuya Chen, Shiwei Du, Huimin Wang, Jian Li, Rong You, Ying Jin, Yongyong Xue, Yan-Qing Guan
Lung is one of important organs and lung diseases seriously affect the health of human beings. In this study, chitosan and gelatin as natural biological macromolecules raw material for the synthesis of ordered colloidal crystal scaffolds (CCS), Fe3 O4 magnetic nanoparticles (MNPs) were used as pore-making for the first time. The pore-making agent were added into the hydrogels to synthesis the ordered (magnetic field) and disordered (no magnetic field) CCS. Collagen and basic fibroblast growth factor (bFGF) modified on the surface of CCS...
January 1, 2019: Colloids and Surfaces. B, Biointerfaces
Marta Martínez-Pérez, Ana Conde, María-Angeles Arenas, Ignacio Mahíllo-Fernandez, Juan-José de-Damborenea, Ramón Pérez-Tanoira, Concepción Pérez-Jorge, Jaime Esteban
OBJECTIVE: Implant-related infection is a devastating complication in orthopedic surgery. Aiming to minimize this problem, many material modifications have been developed. Here we report a study of a surface modification of Ti-6 Al-4 V alloy using a methodology that enables the study of interactions between bacteria and the material in the presence of eukaryotic cells. METHODS: We mixed different concentrations of collection or clinical strains of staphylococci isolated from implant-related infections with preosteoblastic cells using a previously published methodology, analyzing the minimal concentration of bacteria able to colonize the surface of the material through image analysis...
January 1, 2019: Colloids and Surfaces. B, Biointerfaces
Tingting Yang, Donghui Wang, Xuanyong Liu
Titanium and its alloys are widely used in many clinical applications, but implant-associated infection may lead to implant failure. Because of the increasing concern about antibiotic resistant pathogen, photothermal therapy (PTT) as a new treatment strategy has received considerable attention. In this work, gold nanorods (GNRs) photoexcited by the near-infrared (NIR) light were immobilized on Ti surface by electrostatic surface self-assembly technique. Field emission scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) were used to explore the morphology and composition of the GNRs-modified surface...
January 1, 2019: Colloids and Surfaces. B, Biointerfaces
Pearlin Hameed, Vasanth Gopal, Stefan Bjorklund, Ashish Ganvir, Dwaipayan Sen, Nicolaie Markocsan, Geetha Manivasagam
Dissolution of atmospheric plasma sprayed (APS) hydroxyapatite (HAp) coatings on Ti-6Al-4 V medical implants have always been a challenge to overcome in the field of biomedical industry. In the present work, an attempt has been made to develop a HAp coating using a novel thermal spray process called axial suspension plasma spraying (SPS), which leads to thin adherent coatings. Two HAp coatings fabricated by APS (P1 and P2) and four SPS HAp coatings (S1, S2, S3 and S4) produced with varying spraying parameters were characterized in terms of (1) microstructure, porosity, hardness, adhesion strength, contact angle and phase purity; (2) corrosion resistance in 10% Fetal bovine serum (FBS); (3) in-vitro cell adherence and cell viability using human umbilical cord blood-derived mesenchymal stem cells (hMSCs)...
January 1, 2019: Colloids and Surfaces. B, Biointerfaces
Anna Hupalowska, Agnieszka Jedrusik, Meng Zhu, Mark T Bedford, David M Glover, Magdalena Zernicka-Goetz
Nuclear architecture has never been carefully examined during early mammalian development at the stages leading to establishment of the embryonic and extra-embryonic lineages. Heterogeneous activity of the methyltransferase CARM1 during these stages results in differential methylation of histone H3R26 to modulate establishment of these two lineages. Here we show that CARM1 accumulates in nuclear granules at the 2- to 4-cell stage transition in the mouse embryo, with the majority corresponding to paraspeckles...
December 13, 2018: Cell
Ruixia Zhang, Steven Mankoci, Nicholas Walters, Hongyu Gao, Hao Zhang, Xiaoning Hou, Haifeng Qin, Zhencheng Ren, Xianfeng Zhou, Gary L Doll, Ashlie Martini, Nita Sahai, Yalin Dong, Chang Ye
Nickel-titanium (NiTi) alloy is an attractive material for biomedical implant applications. In this study, the effects of laser shock peening (LSP) on the biocompatibility, corrosion resistance, ion release rate and hardness of NiTi were characterized. The cell culture study indicated that the LSP-treated NiTi samples had lower cytotoxicity and higher cell survival rate than the untreated samples. Specifically, the cell survival rate increased from 88 ± 1.3% to 93 ± 1.1% due to LSP treatment. LSP treatment was shown to significantly decrease the initial Ni ion release rate compared with that of the untreated samples...
December 14, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
J Y Chen, Y X Li
Auditory neuropathy is a disorder characterized by absent or severely abnormal auditory brainstem response (ABR) with intact outer hair cell function, as evidenced by the presence of evoked otoacoustic emissions and/or cochlear microphonics. Unlike patients with sensory hearing loss who show clinical evidence of impaired outer hair cell function. For ANSD patients, clinical rehabilitation is mainly limited to hearing aid wearing and cochlear implantation. However, the effect of cochlear implantation for ANSD patients may be difference...
July 2018: Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery
Lijun Deng, Xianhua He, Kenan Xie, Lu Xie, Yi Deng
Defective osteogenesis and latent infections continue to be two major issues in the therapy of bone tissue regeneration. In this study, a unique hierarchically micro/nanoscale-architecture is first proposed and produced on polyetheretherketone (PEEK). Besides, a "simvastatin-PLLA film-tobramycin microspheres" delivery system is subsequently fabricated to endow the PEEK implant with osteogenic and antibacterial capabilities. In vitro antibacterial evaluations confirm that the decorated PEEK scaffolds possess excellent resistance against planktonic/adherent bacteria...
December 14, 2018: Macromolecular Bioscience
Ali Gürkan, Gözde Peker Tekdal, Nagihan Bostancı, Georgios N Belibasakis
AIM: To evaluate the cytokine, chemokine and growth factor levels in peri-implant sulcular fluid (PISF) during healing and osseointegration at osteotomy sites prepared either with piezosurgery (PS) or drills. MATERIALS AND METHODS: Fourteen patients having contralateral partial edentulism in the posterior maxilla were enrolled and 38 osteotomies were prepared. Implants were placed with one-stage surgery. Insertion torque, early healing index, probing depth and modified gingival and plaque indices and crestal bone loss (CBL) were measured...
December 14, 2018: Journal of Periodontology
Elien Peeters, Geert Hooyberghs, Stijn Robijns, Ami De Weerdt, Soňa Kucharíková, Hélène Tournu, Annabel Braem, Katerina Čeh, Gregor Majdič, Tanja Španič, Estera Pogorevc, Birgit Claes, Barbara Dovgan, Lenart Girandon, Frédéric Impellizzeri, Martin Erdtmann, Annika Krona, Jef Vleugels, Mirjam Fröhlich, Jordi Garcia-Forgas, Katrijn De Brucker, Bruno P A Cammue, Karin Thevissen, Patrick Van Dijck, Jozef Vanderleyden, Erik Van der Eycken, Hans P Steenackers
Biofilms, especially those formed by Staphylococcus aureus, play a key role in the development of orthopedic implant infections. Eradication of these infections is challenging due to the elevated tolerance of biofilm cells against antimicrobial agents. In this study, we developed an antibiofilm coating consisting of 5-(4-bromophenyl)-N-cyclopentyl-1-octyl-1H-imidazol-2-amine, designated as LC0024, covalently bound to a titanium implant surface (LC0024-Ti). We showed in vitro that the LC0024-Ti surface reduces biofilm formation of S...
December 13, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Alejandra Velásquez, Edwin Mellisho, Fidel O Castro, Lleretny Rodríguez-Álvarez
The high metabolic activity to which the dairy cattle are exposed for maintain milk production altered steroid metabolism that affect reproductive physiology and reducing oocyte competence. Our aims were 1) to characterize the competence of immature oocytes collected from dairy cattle based on the expression of genes in cumulus cells and 2) to improve oocyte competence to support pre-implantation embryo development by the supplementation of maturation medium with BMP15 and/or AMH. Oocyte donors were identified at the moment of ovary collection and grouped by involuntarily culled dairy cows (Holstein breed) or beef cattle...
December 13, 2018: Molecular Reproduction and Development
Lijing Niu, Chengmin Feng, Chengyi Shen, Bing Wang, Xiaoming Zhang
Postoperative adhesion is a common complication and preventing adhesions during or immediately after operation is particularly important. The application of solid barrier materials represents the most successful clinical strategy to prevent postoperative adhesion. However, a simple physical barrier effect might be insufficient in preventing adhesion satisfactorily. Multilayered structures can be designed with an outer layer as the barrier and an inner layer to respond to relative drug release. In this article, bilayer film composed of a PLGA/PLCA casting layer as barrier and PLGA/PDPA electrospinning layer to respond to the release of anti-fibrosis drug l-Phe was designed and synthesized...
December 13, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Katja Jansen, Miguel Castilho, Sanne Aarts, Michael M Kaminski, Soeren S Lienkamp, Roman Pichler, Jos Malda, Tina Vermonden, Jitske Jansen, Rosalinde Masereeuw
The increasing prevalence of end-stage renal disease and persistent shortage of donor organs call for alternative therapies for kidney patients. Dialysis remains an inferior treatment as clearance of large and protein-bound waste products depends on active tubular secretion. Biofabricated tissues could make a valuable contribution, but kidneys are highly intricate and multifunctional organs. Depending on the therapeutic objective, suitable cell sources and scaffolds must be selected. This study provides a proof-of-concept for stand-alone kidney tubule grafts with suitable mechanical properties for future implantation purposes...
December 13, 2018: Macromolecular Bioscience
Željko Janićijević, Marina Ninkov, Milena Kataranovski, Filip Radovanović
Poly(DL-lactide-co-ε-caprolactone)/poly(acrylic acid) implantable composite reservoirs for cationic drugs are synthesized by sequentially applying photoirradiation and liquid phase inversion. The chemical composition and microstructure of reservoirs are characterized with Fourier transform infrared spectroscopy-attenuated total reflection (FTIR-ATR) and scanning electron microscopy (SEM), respectively. Drug loading and release properties are investigated using methylene blue as the drug model. Biocompatibility of reservoirs is examined through a series of in vitro tests and an in vivo experiment of subcutaneous implantation in Dark Agouti rats...
December 13, 2018: Macromolecular Bioscience
Sarah Brookes, Sherry Voytik-Harbin, Hongji Zhang, Lujuan Zhang, Stacey Halum
OBJECTIVE: Tissue engineering of the larynx requires a complex, multiple tissue layer design. Additionally, spontaneous reinnervation of the larynx after recurrent laryngeal nerve (RLN) injury is often disorganized, resulting in subpar function. This study investigates use of tissue-engineered cartilage and motor endplate-expressing (MEE) tissue-engineered skeletal muscle implants for laryngeal reconstruction and the promotion of organized reinnervation after RLN injury. METHODS: F344 rat primary muscle progenitor cells (MPCs) were isolated...
December 12, 2018: Laryngoscope
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