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https://www.readbyqxmd.com/read/30326062/effects-of-lentivirus-mediated-c3-expression-on-trabecular-meshwork-cells-and-intraocular-pressure
#1
Junkai Tan, Ning Fan, Ningli Wang, BingKai Feng, Ming Yang, Guo Liu, Yun Wang, Xianjun Zhu, Paul L Kaufman, Iok-Hou Pang, Xuyang Liu
Purpose: We evaluated the effects of lentivirus-mediated exoenzyme C3 transferase (C3) expression on cultured primary human trabecular meshwork (HTM) cells in vitro, and on rat intraocular pressure (IOP). Methods: HTM cells were cultured and treated with lentivirus vectors expressing either green fluorescent protein (GFP) only (LV-GFP) or GFP and C3 together (LV-C3-GFP). Changes in cell morphology and actin stress fibers were assessed. The vectors were also injected into the anterior chamber of rats, and GFP expression was visualized by a Micron III Retinal Imaging Microscope in vivo and a fluorescence microscope ex vivo...
October 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/30326036/association-of-angle-width-with-progression-of-normal-tension-glaucoma-a-minimum-7-year-follow-up-study
#2
Ahnul Ha, Young Kook Kim, Jin Wook Jeoung, Dong Myung Kim, Ki Ho Park
Importance: Glaucoma has been dichotomically classified as open or closed angle, and accordingly, distinct therapies have been administered. In this study, the issue of narrow-angle normal-tension glaucoma (NTG), which may be an intermediate-stage or hybrid-stage disease entity, was addressed. Objective: To determine whether anterior chamber (AC) angle width plays any role in NTG progression. Design, Setting, and Participants: Retrospective analysis of prospectively collected data at Seoul National University Hospital between January 2004 and December 2009...
September 20, 2018: JAMA Ophthalmology
https://www.readbyqxmd.com/read/30324000/intraocular-photobonding-to-enable-accommodating-intraocular-lens-function
#3
Nicolas Alejandre-Alba, Rocio Gutierrez-Contreras, Carlos Dorronsoro, Susana Marcos
Purpose: Accommodating intraocular lenses (A-IOLs) require capturing the ciliary muscle forces. Prior work demonstrated strong photo-initiated bonding between strips of capsular bag and poly(2-hydroxyethyl methacrylate); (pHEMA) polymer in an extraocular setting. We demonstrate that photobonding can be achieved intraocularly. Methods: Phacoemulsification was performed in porcine eyes (<24 hours postmortem). A commercial intraocular lens (IOL; pHEMA-MMA material) was inserted in the capsular bag...
September 2018: Translational Vision Science & Technology
https://www.readbyqxmd.com/read/30323760/corneal-blood-staining-after-complicated-cataract-surgery
#4
Mark Krauthammer, Jorje Mandelblum, Oriel Spierer
Purpose: To report the case of a patient with corneal blood staining after a complicated cataract surgery. Observations: We report on a 68-year-old woman who developed corneal blood staining secondary to hyphema after cataract surgery, despite the fact that intraocular pressure was not increased for a prolonged time. The corneal staining spontaneously cleared from the periphery towards the center. It was fully resolved after 2.5 years of follow-up. Conclusions and Importance: In case of hyphema induced by cataract surgery, performance of early anterior chamber washout should be considered to prevent corneal blood staining and poor visual outcome...
September 2018: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/30321154/-surgical-treatment-of-chronic-thromboembolic-pulmonary-hypertension
#5
M A Cherniavskiĭ, A G Edemskiĭ, Iu A Kudaev, A M Cherniavskiĭ
Analysed herein are the results of pulmonary endarterectomy (PEA) performed in a total of 169 patients presenting with chronic thromboembolic pulmonary hypertension (CTEPH) at the National Medical Research Centre named after Academician E.N. Meshalkin over the period from 2004 to 2014. Pulmonary endarterectomy was carried out according to the standard technique in the conditions of assisted circulation with the use of hypothermia and circulatory arrest. The duration of follow up of patients after surgical treatment amounted to 10 years...
2018: Angiologii︠a︡ i Sosudistai︠a︡ Khirurgii︠a︡, Angiology and Vascular Surgery
https://www.readbyqxmd.com/read/30315607/right-atrial-to-left-atrial-volume-index-ratio-is-associated-with-increased-mortality-in-patients-with-pulmonary-hypertension
#6
Manu M Mysore, Kenneth C Bilchick, Priscilla Ababio, Benjamin K Ruth, William C Harding, Khadijah Breathett, Kimberley Chadwell, Brandy Patterson, Hunter Mwansa, Christiana M Jeukeng, Younghoon Kwon, Jamie L W Kennedy, Andrew D Mihalek, Sula Mazimba
BACKGROUND: Pulmonary hypertension (PH) is characterized by increased pulmonary vascular resistance leading to right heart failure. Elevated right atrial (RA) pressure reflects right ventricular (RV) pressure overload and is an established risk factor for mortality in PH. We hypothesized that PH patients with an increased ratio of RA to LA volume index (RAVI/LAVI), would have increased mortality. METHODS: We evaluated the association of RAVI/LAVI with mortality in 124 patients seen at a single academic center's PH clinic after adjusting for the REVEAL risk score, an established risk score in PH...
October 12, 2018: Echocardiography
https://www.readbyqxmd.com/read/30315263/pressure-and-velocity-in-intraocular-and-subarachnoid-space-fluid-chambers-an-inseparable-couple
#7
Hanspeter E Killer, Ruow Hou, Peter Wostyn, Peter Meyer, Achmed Pircher
No abstract text is available yet for this article.
October 12, 2018: Eye
https://www.readbyqxmd.com/read/30312285/management-of-conjunctival-perforation-and-xen-gel-stent-exposure-by-stent-repositioning-through-the-anterior-chamber
#8
Juan Francisco Santamaría-Álvarez, Juan Lillo-Sopena, Silvia Sanz-Moreno, Josep Maria Caminal-Mitjana
In recent years, minimally-invasive glaucoma surgery has emerged as a novel approach to lowering intraocular pressure (IOP). This technique, which is usually reserved for mild to moderate cases, is less invasive than classic filtration surgery and potentially offers a better safety profile. The XEN Gel Stent is implanted through a minimally-invasive, ab interno procedure to create a subconjunctival pathway. Conjunctival tissue disruption is minimal and flow is restricted to avoid hypotony. Data on the optimal approach to managing complications associated with this procedure are limited due to the relatively recent commercialization of this device...
October 11, 2018: Journal of Glaucoma
https://www.readbyqxmd.com/read/30312144/a-novel-fabric-based-versatile-and-stiffness-tunable-soft-gripper-integrating-soft-pneumatic-fingers-and-wrist
#9
Yanqiong Fei, Jiangbei Wang, Wu Pang
This work presents the design and test of a novel fabric-based versatile and stiffness-tunable soft gripper integrating soft pneumatic fingers and wrist. The morphology is designed into a compact tuning fork shape (130 × 110 × 260 mm, 389 g) with two bidirectional sheet-shaped soft fingers and a biaxial bidirectional (universal) cylinder-shaped soft wrist. The multi-degree of freedom of soft fingers and wrist makes the gripper versatile and adaptable to gripping objects of various shapes, sizes, and orientations in a wide range...
October 12, 2018: Soft Robotics
https://www.readbyqxmd.com/read/30311149/cardiac-valve-bioreactor-for-physiological-conditioning-and-hydrodynamic-performance-assessment
#10
Brandon J Tefft, Joshua A Choe, Melissa D Young, Ryan S Hennessy, David W Morse, Jeffery A Bouchard, Herbert J Hedberg, Joseph F Consiglio, Dan Dragomir-Daescu, Robert D Simari, Amir Lerman
PURPOSE: Tissue engineered heart valves (TEHV) are being investigated to address the limitations of currently available valve prostheses. In order to advance a wide variety of TEHV approaches, the goal of this study was to develop a cardiac valve bioreactor system capable of conditioning living valves with a range of hydrodynamic conditions as well as capable of assessing hydrodynamic performance to ISO 5840 standards. METHODS: A bioreactor system was designed based on the Windkessel approach...
October 11, 2018: Cardiovascular Engineering and Technology
https://www.readbyqxmd.com/read/30308357/the-characteristics-of-flame-propagation-in-ammonia-oxygen-mixtures
#11
Qingming Liu, Xu Chen, Jinxiang Huang, Yang Shen, Yunming Zhang, Ziwei Liu
As a carbon free fuel and a hydrogen-energy carrier, ammonia is expected to be one of the promising energy carriers and to be widely used in industries. In this study, the parameters of ammonia/oxygen flame, such as laminar flame speed, the Markstein length, laminar flame thickness, and critical radius of flame instability onset have been investigated experimentally and numerically. A spherical ammonia /oxygen flame propagated in a constant volume chamber and a high speed digital Schilieren photograph system were used in the study...
September 29, 2018: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/30307558/observation-of-wet-specimens-sensitive-to-evaporation-using-scanning-electron-microscopy
#12
Noriyuki Inoue, Yoshiko Takashima, Mitsuo Suga, Toshiaki Suzuki, Yoshikazu Nemoto, Osamu Takai
Wet specimens are notoriously difficult to image in scanning electron microscopes (SEM) owing to evaporation from the required vacuum of the specimen chamber. Traditionally, this issue has been addressed by increasing the specimen chamber pressure. Unfortunately, observation under high specimen chamber pressure cannot prevent the initial evaporation effects. The wet cover method, where the original surface water is retained (and, therefore, considered wet), provides a way to introduce and subsequently image specimens that are sensitive to evaporation within a SEM, while preventing evaporation-related damage, and to observe interesting specimen-water interactions...
October 11, 2018: Microscopy
https://www.readbyqxmd.com/read/30303681/a-male-with-multiple-cardiac-masses
#13
Manasawee Indrabhinduwat, Maria C Arciniegas Calle, Joseph P Colgan, Hector R Villarraga
Summary: Thirty-seven-year-old male presented with cough, dyspnea, significant weight loss (20 kg) and subacute fever for the past 2 months. Physical examination revealed inspiratory and expiratory wheezing bilaterally. A normal S1, S2 and a 3/6 systolic ejection murmur at the left upper parasternal border with respiratory variation were found during cardiac auscultation. Kidney and bone marrow biopsy reported a high-grade B-cell lymphoma. Echocardiography and cardiac CT findings consisted of multiple intracardiac masses affecting the right ventricular (RV) outflow track, RV apex, medial portion of the right atrium and posterior left atrium, as well as mild impairment of the RV systolic function...
September 1, 2018: Echo Research and Practice
https://www.readbyqxmd.com/read/30300309/primary-angle-closure-glaucoma-with-normal-intraocular-pressure-at-the-first-visit-its-prevalence-and-ocular-characteristics
#14
Won Hyuk Oh, Bum Gi Kim, Haksu Kyung, Joo Hwa Lee
PURPOSE: To investigate the prevalence of normal intraocular pressure (IOP) at first visit among patients with primary angle closure glaucoma (PACG) and their ocular characteristics. PATIENTS AND METHODS: We retrospectively reviewed patients with PACG in a referral center. According to untreated IOP, we divided PACG eyes into two groups: those with normal IOP and those with high IOP (>21▒mmHg) at the first visit. RESULTS: One hundred sixty eyes of 160 Korean PACG patients were included...
October 8, 2018: Journal of Glaucoma
https://www.readbyqxmd.com/read/30297481/dry-and-hot-the-hydraulic-consequences-of-a-climate-change-type-drought-for-amazonian-trees
#15
Clarissa G Fontes, Todd E Dawson, Kolby Jardine, Nate McDowell, Bruno O Gimenez, Leander Anderegg, Robinson Negrón-Juárez, Niro Higuchi, Paul V A Fine, Alessandro C Araújo, Jeffrey Q Chambers
How plants respond physiologically to leaf warming and low water availability may determine how they will perform under future climate change. In 2015-2016, an unprecedented drought occurred across Amazonia with record-breaking high temperatures and low soil moisture, offering a unique opportunity to evaluate the performances of Amazonian trees to a severe climatic event. We quantified the responses of leaf water potential, sap velocity, whole-tree hydraulic conductance ( K wt ), turgor loss and xylem embolism, during and after the 2015-2016 El Niño for five canopy-tree species...
October 8, 2018: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/30297073/hemodynamic-pump-patient-interactions-and-left-ventricular-assist-device-imaging
#16
REVIEW
Nikhil Narang, Jayant Raikhelkar, Gabriel Sayer, Nir Uriel
Left ventricular assist devices (LVAD) provide a durable option for patients with advanced hear failure. Axial and centrifugal pump physiology differs with regard to the relationship between pump inflow-outflow cannula pressure differential and flow, which results in device behavior that can vary drastically under different loading conditions. Ramp studies can aid the clinician in choosing the optimal speed to adequately unload the left ventricle. Advances in 3-dimensional echocardiography enhance the understanding of chamber geometry for both types of LVADs...
November 2018: Cardiology Clinics
https://www.readbyqxmd.com/read/30295074/treatment-of-chronic-and-extreme-ocular-hypotension-following-glaucoma-surgery-with-intraocular-platelet-rich-plasma-a-case-report
#17
Omneya Abdalrahman, Alejandra E Rodriguez, Jorge L Alio Del Barrio, Jorge L Alio
PURPOSE: To report a new approach for the treatment of severe ocular hypotony secondary to glaucoma filtering surgery with mitomycin C by injecting autologous eye platelet-rich plasma (E-PRP) in the anterior chamber to block excessive diffuse filtration through an abnormally thinned sclera. METHODS: A 49-year-old patient with the Axenfeld-Rieger syndrome and severe chronic hypotony and corneal edema following filtering glaucoma surgery with mitomycin C received an isolated injection of 0...
October 7, 2018: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/30293652/right-sided-heart-structural-and-functional-remodeling-in-mitral-regurgitation-secondary-to-mitral-valve-prolapse
#18
Laura Iacuzio, Benjamin Essayagh, Filippo Civaia, Elie Dan Schouver, Stephane Rusek, Carinne Dommerc, Christophe Tribouilloy, Gilles Dreyfus, Franck Levy
Preoperative evaluation of the mitral valve but also of tricuspid valve and right ventricular (RV) function is mandatory in primary mitral regurgitation (MR) secondary to mitral valve prolapse (MVP). Tricuspid annulus (TA) diameter plays a pivotal role in the surgical decision to perform preventive combined tricuspid valve annuloplasty. Cardiac magnetic resonance (CMR) is the gold standard for the assessment of RV size and function. Based on 70 consecutive patients (17 women; mean age 64 ± 12) with severe MR secondary to MVP referred for CMR, we sought to assess RV geometry and function and TA dimensions and to study the interaction between TA dilatation and right-sided cardiac chambers...
September 13, 2018: American Journal of Cardiology
https://www.readbyqxmd.com/read/30292602/not-all-lightweight-lead-aprons-and-thyroid-shields-are-alike
#19
Elias Fakhoury, Jo-Ann Provencher, Raja Subramaniam, David J Finlay
OBJECTIVE: With the explosion of minimally invasive surgery, the use of fluoroscopy has significantly increased. Concurrently, there has been a demand for lighter weight aprons. The industry answered this call with the development of lightweight aprons. Our goal was to see whether lighter weight garments provide reduced protection. METHODS: Dry laboratory testing was performed in a standard X-ray room, using a standard fluoroscopy table and standard acrylic blocks...
October 3, 2018: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/30289177/an-ex-vivo-bladder-model-with-detrusor-smooth-muscle-removed-to-analyze-biologically-active-mediators-released-from-the-suburothelium
#20
Leonie Durnin, Benjamin Kwok, Priya Kukadia, Roisin McAvera, Robert D Corrigan, Sean M Ward, Ying Zhang, Qi Chen, Sang Don Koh, Kenton M Sanders, Violeta N Mutafova-Yambolieva
A KEY POINT LIST: Studies of urothelial cells, bladder sheets or lumens of filled bladders have suggested that mediators released from urothelium into suburothelium (SubU)/lamina propria (LP) activate mechanisms controlling detrusor excitability. None of these approaches, however, has enabled direct assessment of availability of mediators at SubU/LP during filling. We developed an ex vivo mouse bladder preparation with intact urothelium and SubU/LP but no detrusor, which allows direct access to the SubU/LP surface of urothelium during filling...
October 5, 2018: Journal of Physiology
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