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Peihong Xue, Wendong Liu, Zhongyi Gu, Xingchi Chen, Jingjie Nan, Junhu Zhang, Hongchen Sun, Zhanchen Cui, Bai Yang
Gradient biomaterials have shown enormous potential in high-throughput screening of biomaterials and material-induced cell migration. To make the screening process more rapid and precise, improving the regularity of morphological structure and chemical modification on gradient biomaterials have attract much attention. In this paper, we present a novel fabrication strategy to introduce ordered nanopattern arrays into gradient biomaterials, through combining surface-initiated atom transfer radical polymerization (SI-ATRP) and inclined reactive ion etching (RIE) based on colloidal lithography...
December 5, 2018: ACS Applied Materials & Interfaces
Yunchao Xie, Chi Zhang, Jheng-Wun Su, Heng Deng, Cheng Zhang, Jian Lin
To date, various approaches to synthesize zeolitic imidazole frameworks (ZIFs) have been developed, such as solvothermal, sonochemical, microfluidic, and mechanochemistry reactions. However, most of them are time-consuming and involve complex processing steps, thus they cannot rapidly screen potential candidates to obtain ZIFs on demand. Such a challenge calls for efficient synthetic approaches. Herein, we overcome this challenge by employing two nonconventional heating strategies, i.e., microwave- and Joule-heating, which are induced by laser-induced graphene (LIG) microreactors, for rapidly synthesizing ZIFs...
December 4, 2018: ChemSusChem
Yingying Zhang, Yanghui Xing, Jian Li, Zhiqiang Zhang, Huiqin Luan, Zhaowei Chu, He Gong, Yubo Fan
Osteogenic differentiation of cells has considerable clinical significance in bone defect treatment, and cell behavior is linked to extracellular matrix stiffness. This study aimed to determine how matrix stiffness affects cell morphology and subsequently regulates the osteogenic phenotype of osteogenesis precursor cells. Four PDMS substrates were prepared with stiffness corresponding to the elastic modulus ranging from 0.6 MPa to 2.7 MPa by altering the Sylgard 527 and Sylgard 184 concentrations. MC3T3-E1 cells were cultured on the matrices...
2018: BioMed Research International
Gonzalo Artiach, Miguel Carracedo, Joan Clària, Andres Laguna-Fernandez, Magnus Bäck
Intimal hyperplasia remains a significant clinical problem in for example coronary artery bypass graft failure. Since omega-3 fatty acids reduce intimal hyperplasia, we hypothesized that the G protein-coupled receptor ChemR23 for the omega-3-derived pro-resolving lipid mediator resolvin E1 drives those effects. ChemR23+/+ and ChemR23-/- mice were generated with or without introduction of the Caenorhabditis elegans fat-1 transgene, which leads to an endogenous omega-3 fatty acid synthesis and thus increasing the substrate for resolvin E1 formation...
2018: Frontiers in Pharmacology
Zongyang Lv, Lingmin Yuan, James H Atkison, Katelyn M Williams, Ramir Vega, E Hampton Sessions, Daniela B Divlianska, Christopher Davies, Yuan Chen, Shaun K Olsen
E1 enzymes activate ubiquitin (Ub) and ubiquitin-like modifiers (Ubls) in the first step of Ub/Ubl conjugation cascades and represent potential targets for therapeutic intervention in cancer and other life-threatening diseases. Here, we report the crystal structure of the E1 enzyme for the Ubl SUMO in complex with a recently discovered and highly specific covalent allosteric inhibitor (COH000). The structure reveals that COH000 targets a cryptic pocket distinct from the active site that is completely buried in all previous SUMO E1 structures and that COH000 binding to SUMO E1 is accompanied by a network of structural changes that altogether lock the enzyme in a previously unobserved inactive conformation...
December 4, 2018: Nature Communications
Rebecca Kaye, Nora Botten, Marit Lippestad, Dayu Li, Robin R Hodges, Tor P Utheim, Charles N Serhan, Darlene A Dartt
PURPOSE: To identify interactions of the epidermal growth factor receptor (EGFR) with the pro-resolving mediator receptors for RvD1 and RvE1 to stimulate an increase in intracellular [Ca2+ ] ([Ca2+ ]i ) and mucin secretion from cultured human and rat conjunctival goblet cells. METHODS: Goblet cells from human and rat conjunctiva were grown in culture using RPMI media. Cultured goblet cells were pre-incubated with inhibitors, and then stimulated with RvD1, RvE1, EGF or the cholinergic agonist carbachol (Cch)...
December 1, 2018: Experimental Eye Research
Ruili Liu, Xiang Yao, Xiangnan Liu, Jiandong Ding
Cellular responses on a topographic surface are fundamental topics about Interfaces and Biology. Herein, a poly(lactide-co-glycolide) (PLGA) micropillar array was prepared and found to trigger significant self deformation of cell nuclei. The time-dependent cell viability and thus cell proliferation was investigated. Despite of significant nuclear deformation, all of the examined cell types (Hela, HepG2, MC3T3-E1 and NIH3T3) could survive and proliferate on the micropillar array, yet exhibited different proliferation abilities...
December 4, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Cristina Guarducci, Martina Bonechi, Matteo Benelli, Chiara Biagioni, Giulia Boccalini, Dario Romagnoli, Roberto Verardo, Rachel Schiff, C Kent Osborne, Carmine De Angelis, Angelo Di Leo, Luca Malorni, Ilenia Migliaccio
CDK4/6 inhibitors represent a new treatment standard for hormone receptor-positive (HR+), HER2-negative advanced breast cancer (BC) patients. Although efficacious, resistance to these agents is universal. Here, we profiled a large panel of HR+ BC cell lines with conditioned resistance to the CDK4/6 inhibitor palbociclib, and analyzed cell cycle-related markers by gene expression profiles (GEP) and western blot (WB). GEP showed high molecular heterogeneity among the models, with E2F targets being significantly enriched both during treatment and at the time of resistance...
2018: NPJ Breast Cancer
Kyung-Sun Lee, Suhng-Wook Kim, Hyeong-Seon Lee
Orostachys japonicus ( O. japonicus ) is utilized as a traditional medicine for patients with various diseases. This study investigated the effect of the ethyl acetate fraction from O. japonicus extract (OJE) on the growth inhibition of OVCAR-3 human ovarian cancer cells demonstrated to inhibit cell growth and arrest the cell cycle in OVCAR-3 cells by blocking the sub-G1 phase and decreasing cyclin E1/CDK2 expression. Cell cycle arrest was connected to the increased expression of the cell cycle regulating factors p53 and p21...
November 2018: Food Science & Nutrition
Seok-Woo Park, Sang-Kyeom Kim, Hyung-Suk Lee, Eun-Song Lee, Elbert de Josselin de Jong, Baek-Il Kim
BACKGROUND: This study compared two fluorescence parameters (fluorescence loss [ΔF] and red fluorescence gain [ΔR]) among three generations of quantitative light-induced fluorescence (QLF) systems with the aim of determining the validities of these parameters in the three devices for differentiating the severity of enamel caries. METHODS: Forty-one extracted human premolars and molars with suspected enamel caries were selected. Fluorescence images of all teeth were obtained using first-, second-, and third-generation QLF systems (Inspektor Pro, QLF-D, and Qraycam, respectively)...
November 30, 2018: Photodiagnosis and Photodynamic Therapy
Seyyed Mehdy Elahi, Chun Fang Shen, Rénald Gilbert
During the last decade, oncolytic viruses such as vesicular stomatitis virus (VSV) have gained tremendous popularity as efficient vaccines for infectious diseases as well as for the treatment of cancer. Our laboratory has developed two stable cell lines, 293SF-3F6 (derived from HEK293 A cells) and SF-BMAdR cells (a variant of A549 that expresses the E1 region of human adenovirus). These two cell lines were adapted to grow efficiently in suspension culture and in serum-free medium. In this report we evaluated the production of a recombinant VSV expressing the green fluorescent protein (VSV-GFP) in these two stable cell lines...
November 30, 2018: Journal of Biotechnology
Seong-Yeong Heo, Seok-Chun Ko, Gun-Woo Oh, Namwon Kim, Il-Whan Choi, Won Sun Park, Won-Kyo Jung
Fish bone extract (FBE) containing a trioligopeptide (FBP-KSA, Lys-Ser-Ala) isolated from Johnius belengerii could induce osteogenic activities on MC3T3-E1 pre-osteoblasts in our previous study. Regarding the osteogenic effect of FBE, in the present study, we fabricated the three-dimensional (3D) interconnected polycaprolactone (PCL)/FBE scaffolds for bone tissue regeneration. After fabrication of PCL scaffolds using 3D printing, FBE was coated on the surface of PCL scaffolds by self-assembly process. In the physical characteristic and mechanical property tests, the results demonstrated that the fabricated scaffolds have the strut diameter (between 340 and 345 μm), pore size (between 470 and 480 μm), porosity (between 50% and 55%), and tensile properties (Young's modulus: 9...
December 3, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Michael J Wiegand, Gregory W Kubacki, Jeremy L Gilbert
Electrochemical interactions at the cell-metal interface determine cell viability and influence behavior in response to different electrode potential conditions, specifically cathodic biases. Mechanically assisted crevice corrosion, for example, induces cathodic potentials and the associated electrochemical consequences of increased reduction reactions at the implant surface may affect cell viability in a manner that is different for various cell phenotypes. Monocyte macrophage-like U937 cells were cultured on cobalt-chromium-molybdenum (CoCrMo) metal surfaces in vitro for 24 h to assess cell behavior in response to sustained applied voltages...
December 3, 2018: Journal of Biomedical Materials Research. Part A
Zhiqin Deng, Wencui Li, Jianying Xu, Meishen Yu, Duan Li, Qiuchan Tan, Daping Wang, Lixin Chen, Liwei Wang
Evidence has been reported by us and others supporting the important roles of chloride channels in a number of osteoblast cell functions. The ClC-3 chloride channel is activated by estradiol binding to estrogen receptor alpha on the cell membranes of osteoblasts. However, the functions of these chloride channels in estrogen regulation of osteoblast metabolism remain unclear. In the present study, the roles of chloride channels in estrogen regulation of osteoblasts were investigated in the osteoblastic cell line MC3T3-E1...
December 2, 2018: Journal of Cellular Biochemistry
Emad Hamdy Gad, Eslam Ayoup, Yasmin Kamel, Talat Zakareya, Mohamed Abbasy, Ali Nada, Mohamed Housseni, Mohammed Al-Sayed Abd-Elsamee
Objectives: Laparoscopic cholecystectomy - associated bile duct injury is a clinical problem with bad outcome. The study aimed to analyze the outcome of surgical management of these injuries. Patients and methods: We retrospectively analyzed 69 patients underwent surgical management of laparoscopic cholecystectomy related major bile duct injuries in the period from the beginning of 2013 to the beginning of 2018. Results: Regarding injury type; the Leaking, Obstructing, leaking + obstructing, leaking + vascular, and obstructing + vascular injuries were 43...
December 2018: Annals of Medicine and Surgery
Akari Nitta, Kazuya Hori, Isei Tanida, Ayumi Igarashi, Yasuyo Deyama, Takashi Ueno, Eiki Kominami, Manabu Sugai, Koji Aoki
Autophagy, a system for the bulk degradation of intracellular components, is essential for homeostasis and the healthy physiology and development of cells and tissues. Its deregulation is associated with human disease. Thus, methods to modulate autophagic activity are critical for analysis of its role in mammalian cells and tissues. Here we report a method to inhibit autophagy using a mutant variant of the protein ATG7, a ubiquitin E1-like enzyme essential for autophagosome formation. During autophagy, ATG7 activates the conjugation of LC3 (ATG8) with phosphatidylethanolamine (PE) and ATG12 with ATG5...
November 30, 2018: Biochemical and Biophysical Research Communications
Lei Wang, Jian Cheng, Zhen Hua, Mingming Liu, Haiyan Xu, Yong Ma, Guicheng Huang, Guoqing Mao
α-melanocyte stimulating hormone (α-MSH) is a member of the melanocortin family, that has displayed important biological functions in diverse cells and tissues. The purpose of this study is to test the effect of α-MSH on the differentiation and mineralization of osteoblast cells. The expression of the α-MSH membrane receptor MC1R but not MC2R, MC3R, or MC4R increased distinctively during the osteogenic differentiation from 3, 7 to 14 d. Treatment with α-MSH promoted the differentiation and mineralization of MC3T3-E1 cells by increasing the activity of ALP, enhancing Alizarin Red S staining, and stimulating the expression of osteogenic genes, including ALP1, osteocalcin (Bglap2), and osterix (Sp7)...
November 26, 2018: European Journal of Pharmacology
Daniel Owusu, Scott R Weaver, Bo Yang, David L Ashley, Lucy Popova
OBJECTIVES: To evaluate recent levels and trends in trust in sources of health information on e-cigarettes in the United States. METHODS: We obtained data from nationally representative samples of adults in 2015 (n = 5389), 2016 (n = 5273), and 2017 (n = 5389) that reported trust in 13 sources of health information on e-cigarettes in the United States. We used weighted linear regression models to examine temporal trends in trust levels. RESULTS: Doctors, health organizations, the Centers for Disease Control and Prevention (CDC), health experts and scientists, the National Institutes of Health (NIH), the Food and Drug Administration (FDA), and family and friends were trusted, whereas news media, e-cigarette users, social media, vape shop employees, and e-cigarette and cigarette companies were distrusted...
November 29, 2018: American Journal of Public Health
Arthur G Cosby, M Maya McDoom-Echebiri, Wesley James, Hasna Khandekar, Willie Brown, Heather L Hanna
OBJECTIVES: To examine 47 years of US urban and rural mortality trends at the county level, controlling for effects of education, income, poverty, and race. METHODS: We obtained (1) Centers for Disease Control and Prevention WONDER (Wide-ranging ONline Data for Epidemiologic Research) data (1970-2016) on 104 million deaths; (2) US Census data on education, poverty, and race; and (3) Bureau of Economic Analysis data on income. We calculated ordinary least square regression models, including interaction models, for each year...
November 29, 2018: American Journal of Public Health
Neal D Goldstein, Joanna S Suder, Jonathan Purtle
OBJECTIVES: To examine trends and characteristics of proposed and enacted state legislation that would directly affect states' immunization exemption laws. METHODS: We performed content analysis of proposed bills in state legislatures from 2011 to 2017. We classified bills as provaccination or antivaccination. RESULTS: State legislators proposed 175 bills, with the volume increasing over time: 92 (53%) bills expanded access to exemptions, and 83 (47%) limited the ability to exempt...
November 29, 2018: American Journal of Public Health
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