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Red clover

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https://www.readbyqxmd.com/read/30477428/analysis-of-transcripts-and-splice-isoforms-in-red-clover-trifolium-pratense-l-by-single-molecule-long-read-sequencing
#1
Yuehui Chao, Jianbo Yuan, Sifeng Li, Siqiao Jia, Liebao Han, Lixin Xu
BACKGROUND: Red clover (Trifolium pratense L.) is an important cool-season legume plant, which is the most widely planted forage legume after alfalfa. Although a draft genome sequence was published already, the sequences and completed structure of mRNA transcripts remain unclear, which limit further explore on red clover. RESULTS: In this study, the red clover transcriptome was sequenced using single-molecule long-read sequencing to identify full-length splice isoforms, and 29,730 novel isoforms from known genes and 2194 novel isoforms from novel genes were identified...
November 26, 2018: BMC Plant Biology
https://www.readbyqxmd.com/read/30468138/growth-performance-and-carcass-traits-of-steers-finished-on-three-different-systems-including-legume-grass-pasture-and-grain-diets
#2
A P B Fruet, F S Stefanello, F Trombetta, A N M De Souza, A G Rosado Júnior, C J Tonetto, J L C Flores, R B Scheibler, R M Bianchi, P S Pacheco, A De Mello, J L Nörnberg
Inclusion of legume in grass pastures optimizes protein values of the forage and promotes improved digestibility. Therefore, we hypothesized that finishing steers on a novel combination of legumes and grass pasture would produce carcasses with acceptable traits when compared to carcasses from steers finished in feedlot systems. In this study, we evaluated the effects of finishing steers on three systems including: grazing legume-grass pasture containing oats, ryegrass, white and red clover (PAST), grazing PAST plus supplementation with whole corn grain (14 g/kg BW (SUPP)), and on a feedlot-confined system with concentrate only (28 g/kg BW, consisting of 850 g/kg of whole corn grain and 150 g/kg of protein-mineral-vitamin supplement (GRAIN)) on growth performance of steers, carcass traits and digestive disorders...
November 23, 2018: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/30408053/temporal-changes-in-genetic-diversity-and-forage-yield-of-perennial-ryegrass-in-monoculture-and-in-combination-with-red-clover-in-swards
#3
Christophe Verwimp, Tom Ruttink, Hilde Muylle, Sabine Van Glabeke, Gerda Cnops, Paul Quataert, Olivier Honnay, Isabel Roldán-Ruiz
Agricultural grasslands are often cultivated as mixtures of grasses and legumes, and an extensive body of literature is available regarding interspecific interactions, and how these relate to yield and agronomic performance. However, knowledge of the impact of intraspecific diversity on grassland functioning is scarce. We investigated these effects during a 4-year field trial established with perennial ryegrass (Lolium perenne) and red clover (Trifolium pratense). We simulated different levels of intraspecific functional diversity by sowing single cultivars or by combining cultivars with contrasting growth habits, in monospecific or bispecific settings (i...
2018: PloS One
https://www.readbyqxmd.com/read/30363087/bioconversion-of-5-deoxystrigol-stereoisomers-to-monohydroxylated-strigolactones-by-plants
#4
Kotomi Ueno, Hitomi Nakashima, Masaharu Mizutani, Hirosato Takikawa, Yukihiro Sugimoto
The bioconversion of 5-deoxystrigol (5DS) and 4-deoxyorobanchol (4DO), the simplest canonical strigolactones (SLs), into monohydroxylated SLs such as strigol, sorgomol and orobanchol was confirmed by administering of stable isotope-labeled substrates to hydroponically grown plants. Liquid chromatography-mass spectrometry analyses established that 5DS was stereoselectively converted into strigol and sorgomol by cotton ( Gossypium hirsutum ) and Chinese milk vetch ( Astragalus sinicus ), respectively. 4DO was converted into orobanchol by rice ( Oryza sativa )...
August 20, 2018: Journal of Pesticide Science
https://www.readbyqxmd.com/read/30335760/effects-of-red-clover-isoflavones-on-tall-fescue-seed-fermentation-and-microbial-populations-in-vitro
#5
Emily A Melchior, Jason K Smith, Liesel G Schneider, J Travis Mulliniks, Gary E Bates, Zachary D McFarlane, Michael D Flythe, James L Klotz, Jack P Goodman, Huihua Ji, Phillip R Myer
Negative impacts of endophyte-infected Lolium arundinaceum (Darbyshire) (tall fescue) are responsible for over $2 billion in losses to livestock producers annually. While the influence of endophyte-infected tall fescue has been studied for decades, mitigation methods have not been clearly elucidated. Isoflavones found in Trifolium pratense (red clover) have been the subject of recent research regarding tall fescue toxicosis mitigation. Therefore, the aim of this study was to determine the effect of ergovaline and red clover isoflavones on rumen microbial populations, fiber degradation, and volatile fatty acids (VFA) in an in vitro system...
2018: PloS One
https://www.readbyqxmd.com/read/30326221/formononetin-and-biochanin-a-protects-against-ritonavir-induced-hepatotoxicity-via-modulation-of-nf%C3%AE%C2%BAb-pakt-signaling-molecules
#6
Alauddin, Swati Chaturvedi, Mohd Yaseen Malik, Lubna Azmi, Ila Shukla, Zaiba Naseem, ChandanaVenkateswara Rao, Naresh Kumar Agarwal
AIMS: Ritonavir (RIT) is a human immune deficiency virus (HIV) protease inhibitor (PI) active against HIV-1 and HIV-2. Among various adverse effects of PIs, hepatotoxicity is a very common adverse reaction of RIT which is concentration dependent. Red clover isoflavones are found to possess anti-inflammatory, antioxidant and anti-apoptosis activity. Furthermore, recent studies have demonstrated that these isoflavones can be used to alleviate the side-effects of drugs. Hence, the present study was inquested to ascertain the effect of Formononetin (FMN) and Biochanin A (BCA) on RIT induced hepatotoxicity...
October 13, 2018: Life Sciences
https://www.readbyqxmd.com/read/30283417/using-omic-approaches-to-compare-temporal-bacterial-colonization-of-lolium-perenne-lotus-corniculatus-and-trifolium-pratense-in-the-rumen
#7
Christopher L Elliott, Joan E Edwards, Toby J Wilkinson, Gordon G Allison, Kayleigh McCaffrey, Mark B Scott, Pauline Rees-Stevens, Alison H Kingston-Smith, Sharon A Huws
Understanding rumen plant-microbe interactions is central for development of novel methodologies allowing improvements in ruminant nutrient use efficiency. This study investigated rumen bacterial colonization of fresh plant material and changes in plant chemistry over a period of 24 h period using three different fresh forages: Lolium perenne (perennial ryegrass; PRG), Lotus corniculatus (bird's foot trefoil; BFT) and Trifolium pratense (red clover; RC). We show using 16S rRNA gene ion torrent sequencing that plant epiphytic populations present pre-incubation (0 h) were substantially different to those attached post incubations in the presence of rumen fluid on all forages...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/30272805/cadmium-uptake-by-ryegrass-and-ryegrass-clover-mixtures-under-different-liming-rates
#8
Ebrahim Benyas, Jennifer Owens, Salome Seyedalikhani, Brett Robinson
Cadmium accumulates in soils that receive repeated applications of Cd-rich superphosphate fertilizers. There is evidence that adding clovers to a crop solubilizes soil Cd, increasing the bioavailability of Cd. This can lead to high plant Cd concentrations. This research aimed to test whether liming-induced increases in pH in mixed crops of clovers and ryegrasses reduced forage Cd concentrations. A greenhouse pot trial applied lime at three rates (0, 1, and 2% of soil dry weight) to eight different plant treatments-four as monocultures (perennial ryegrass [ L...
September 2018: Journal of Environmental Quality
https://www.readbyqxmd.com/read/30269660/effects-of-red-clover-on-perimenopausal-and-postmenopausal-women-s-blood-lipid-profile-a-meta-analysis
#9
 Luís, F Domingues, L Pereira
The study aimed to perform a systematic review with meta-analysis of randomized controlled trials (RCTs) to access the effects of red clover isoflavones on the blood lipid profile of both perimenopausal and postmenopausal women. PubMed, Web of Science, Scopus, SciELO, and Cochrane Library were searched for the terms 'red clover', 'Trifolium pratense', and 'randomized controlled trial' during November 2017. Summary measures were reported as weighted mean differences (WMD) with 95% confidence interval (CI). Fixed or random effects models were used for meta-analyses, according to heterogeneity...
October 2018: Climacteric: the Journal of the International Menopause Society
https://www.readbyqxmd.com/read/30261641/binding-of-red-clover-isoflavones-to-actin-as-a-potential-mechanism-of-anti-metastatic-activity-restricting-the-migration-of-cancer-cells
#10
Grażyna Budryn, Joanna Grzelczyk, Horacio Pérez-Sánchez
Actin functions are crucial for the ability of the cell to execute dynamic cytoskeleton reorganization and movement. Nutraceuticals that form complexes with actin and reduce its polymerization can be used in cancer therapy to prevent cell migration and metastasis of tumors. The aim of this study was to evaluate the ability of isoflavones to form complexes with actin. Docking simulation and isothermal titration calorimetry were used for this purpose. The formation of complexes by hydrogen bonds, hydrophobic and π-π interactions was demonstrated...
September 26, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/30250677/annual-flower-strips-support-pollinators-and-potentially-enhance-red-clover-seed-yield
#11
Maj Rundlöf, Ola Lundin, Riccardo Bommarco
Ecological intensification provides opportunity to increase agricultural productivity while minimizing negative environmental impacts, by supporting ecosystem services such as crop pollination and biological pest control. For this we need to develop targeted management solutions that provide critical resources to service-providing organisms at the right time and place. We tested whether annual strips of early flowering phacelia Phacelia tanacetifolia support pollinators and natural enemies of seed weevils Protapion spp...
August 2018: Ecology and Evolution
https://www.readbyqxmd.com/read/30199256/irilone-from-red-clover-trifolium-pratense-potentiates-progesterone-signaling
#12
Jung-Ho Lee, Matthew Dean, Julia R Austin, Joanna E Burdette, Brian T Murphy
The use of botanical dietary supplements is becoming increasingly popular for the alleviation of hormonal-based conditions such as hot flashes, premenstrual syndrome, and fertility. Estrogen and progesterone receptors (ER and PR) play an essential role in these processes. However, despite the fact that many therapies used to alleviate gynecological conditions act through PR-mediated mechanisms, few studies have investigated or identified any herbal natural product components that act on this receptor. In the current study, we used a progesterone response element (PRE)-luciferase (Luc) reporter assay to identify four phytoprogestins present in a standardized red clover ( Trifolium pratense) extract...
September 28, 2018: Journal of Natural Products
https://www.readbyqxmd.com/read/30169907/hijacking-a-host-scaffold-protein-rack1-for-replication-of-a-plant-rna-virus
#13
Kiwamu Hyodo, Nobuhiro Suzuki, Tetsuro Okuno
Receptor for activated C kinase 1 (RACK1) is strictly conserved across eukaryotes and acts as a versatile scaffold protein involved in various signaling pathways. Plant RACK1 is known to exert important functions in innate immunity against fungal and bacterial pathogens. However, the role of the RACK1 in plant-virus interactions remains unknown. Here, we addressed the role of RACK1 of Nicotiana benthamiana during infection by red clover necrotic mosaic virus (RCNMV), a plant positive-stranded RNA virus. NbRACK1 was shown to be recruited by the p27 viral replication protein into endoplasmic reticulum-derived aggregated structures (possible replication sites)...
August 31, 2018: New Phytologist
https://www.readbyqxmd.com/read/30159684/complete-nucleotide-sequence-and-genome-organization-of-red-clover-associated-virus-1-rcav1-a-putative-member-of-the-genus-waikavirus-family-secoviridae-order-picornavirales
#14
Igor Koloniuk, Jana Fránová
Using high-throughput sequencing, a novel waikavirus was identified in a mixed virus infection of red clover (Trifolium pratense L.). Its complete genomic sequence was determined and characterized. The virus, tentatively named red clover associated virus 1 (RCaV1), is phylogenetically related to members of the genus Waikavirus (family Secoviridae, order Picornavirales).
December 2018: Archives of Virology
https://www.readbyqxmd.com/read/30150852/transcriptome-analysis-of-leaf-senescence-in-red-clover-trifolium-pratense-l
#15
Yuehui Chao, Lijuan Xie, Jianbo Yuan, Tao Guo, Yinruizhi Li, Fengqi Liu, Liebao Han
Red clover ( Trifolium pratense L.) is an important cool-season legume plant, which is used as forage. Leaf senescence is a critical developmental process that negatively affects plant quality and yield. The regulatory mechanism of leaf senescence has been studied, and genes involved in leaf senescence have been cloned and characterized in many plants. However, those works mainly focused on model plants. Information about regulatory pathways and the genes involved in leaf senescence in red clover is very sparse...
September 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/30143875/red-clover-associated-luteovirus-a-newly-classifiable-member-of-the-genus-luteovirus-with-an-enamo-like-p5-protein
#16
Ondřej Lenz, Tatiana Sarkisová, Igor Koloniuk, Jana Fránová, Jaroslava Přibylová, Josef Špak
This study reports the complete genomic sequence of a novel virus isolated from red clover. According to its genomic organization, its similarity to luteoviruses, and a greater than 10% difference in all genes, this virus isolate likely represents a new luteovirus species. As seen in nectarine stem pitting-associated virus (NSPaV) and NSPaV-South Korea (SK) luteoviruses, it differs from typical luteoviruses through the absence of ORF3a and ORF4 encoding movement proteins. Furthermore, its P5 protein (responsible for aphid transmission) is more similar to the P5 of enamoviruses than that of luteoviruses...
December 2018: Archives of Virology
https://www.readbyqxmd.com/read/30132047/effects-of-isoflavones-on-breast-tissue-and-the-thyroid-hormone-system-in-humans-a-comprehensive-safety-evaluation
#17
REVIEW
S Hüser, S Guth, H G Joost, S T Soukup, J Köhrle, L Kreienbrock, P Diel, D W Lachenmeier, G Eisenbrand, G Vollmer, U Nöthlings, D Marko, A Mally, T Grune, L Lehmann, P Steinberg, S E Kulling
Isoflavones are secondary plant constituents of certain foods and feeds such as soy, linseeds, and red clover. Furthermore, isoflavone-containing preparations are marketed as food supplements and so-called dietary food for special medical purposes to alleviate health complaints of peri- and postmenopausal women. Based on the bioactivity of isoflavones, especially their hormonal properties, there is an ongoing discussion regarding their potential adverse effects on human health. This review evaluates and summarises the evidence from interventional and observational studies addressing potential unintended effects of isoflavones on the female breast in healthy women as well as in breast cancer patients and on the thyroid hormone system...
September 2018: Archives of Toxicology
https://www.readbyqxmd.com/read/30125211/cropping-system-diversification-reduces-severity-and-incidence-of-soybean-sudden-death-syndrome-caused-by-fusarium-virguliforme
#18
L F S Leandro, S Eggenberger, C Chen, J Williams, G A Beattie, M Liebman
Current management of sudden death syndrome (SDS) of soybean, caused by Fusarium virguliforme, focuses on planting resistant varieties and improving soil drainage; however, these measures are not completely effective. A 6-year study evaluated the effects of cropping system diversification on SDS and soybean yield. SDS, root health, yield, and F. virguliforme density in soil were assessed in a naturally infested field trial comparing a 2-year cropping system consisting of a corn-soybean rotation and synthetic fertilizer applications with 3- and 4-year cropping systems consisting of corn-soybean-oat + red clover and corn-soybean-oat +alfalfa-alfalfa rotations, respectively, with both manure and low synthetic fertilizer rates...
September 2018: Plant Disease
https://www.readbyqxmd.com/read/30122409/grazing-of-dairy-cows-on-pasture-versus-indoor-feeding-on-total-mixed-ration-effects-on-low-moisture-part-skim-mozzarella-cheese-yield-and-quality-characteristics-in-mid-and-late-lactation
#19
Arunima Gulati, Norann Galvin, Deirdre Hennessy, Stephen McAuliffe, Michael O'Donovan, Jennifer J McManus, Mark A Fenelon, Timothy P Guinee
This study investigated the effects of 3 dairy cow feeding systems on the composition, yield, and biochemical and physical properties of low-moisture part-skim Mozzarella cheese in mid (ML; May-June) and late (LL; October-November) lactation. Sixty spring-calving cows were assigned to 3 herds, each consisting of 20 cows, and balanced on parity, calving date, and pre-experimental milk yield and milk solids yield. Each herd was allocated to 1 of the following feeding systems: grazing on perennial ryegrass (Lolium perenne L...
August 16, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/30107410/feeding-and-reproductive-hosts-of-spissistilus-festinus-say-hemiptera-membracidae-found-in-californian-vineyards
#20
Cindy R Preto, Mysore R Sudarshana, Frank G Zalom
Grapevine red blotch virus (GRBV) currently poses a serious threat to the wine industry. The three-cornered alfalfa hopper (3CAH), Spissistilus festinus (Say) (Hemiptera: Membracidae), was recently shown in a greenhouse study to be a vector of GRBV in grapes. Feeding and reproductive status of 10 weed and 10 cover crop species commonly found in grape vineyards were evaluated in no-choice tests. Species confirmed as reproductive hosts were subsequently evaluated in choice tests to determine S. festinus oviposition preference...
August 10, 2018: Journal of Economic Entomology
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