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Eoin Byrne, Krisztina Kovacs, Ed W J van Niel, Karin Willquist, Sven-Erik Svensson, Emma Kreuger
Background: Current EU directives demand increased use of renewable fuels in the transportation sector but restrict governmental support for production of biofuels produced from crops. The use of intercropped lucerne and wheat may comply with the directives. In the current study, the combination of ensiled lucerne ( Medicago sativa L.) and wheat straw as substrate for hydrogen and methane production was investigated. Steam-pretreated and enzymatically hydrolysed wheat straw [WSH, 76% of total chemical oxygen demand (COD)] and ensiled lucerne (LH, 24% of total COD) were used for sequential hydrogen production through dark fermentation and methane production through anaerobic digestion and directly for anaerobic digestion...
2018: Biotechnology for Biofuels
Malgorzata Szumacher-Strabel, Anna Stochmal, Adam Cieslak, Martyna Kozłowska, Dawid Kuznicki, Mariusz Kowalczyk, Wieslaw Oleszek
BACKGROUND: Alfalfa (Medicago sativa) is an important legume used in animal feed. The primary objective in this study was to analyze and trace the individual saponins in fresh and ensiled alfalfa of ten varieties, with the aim of evaluating the structural changes undergone by saponins during ensiling. A secondary objective was to examine whether any of the changes in saponin content were associated with changes in the basic nutrient composition of the ensiled alfalfa. RESULTS: The total saponin concentration increased when the fresh alfalfa was processed into silage...
October 16, 2018: Journal of the Science of Food and Agriculture
Lei Chen, Zhihao Dong, Junfeng Li, Tao Shao
BACKGROUND: Silages produced with grass and legume mixtures may have positive associative effects on silage quality, and animal performance when ruminants are fed the silages. The objective of this study was to determine the preservation characteristics, in vitro rumen fermentation profile and microbial abundance of silages, produced with mixtures of sweet sorghum (SS) and alfalfa (AF) in different ratios: 100: 0 (Control), 75: 25 (SA25), 50: 50 (SA50), 25: 75 (SA75) and 0: 100 (SA100) on a fresh weight basis...
October 9, 2018: Journal of the Science of Food and Agriculture
Azizza Sifeeldein, Siran Wang, Junfeng Li, Zhihao Dong, Lei Chen, Niaz Ali Kaka, Tao Shao
AIM: to isolate, screen and identify the lactic acid bacteria (LAB) from elephant silage during the process of the fermentation and their effects on the fermentation quality of sweet sorghum silage. METHODS AND RESULTS: The isolated strains were identified based on morphological, physiological and biochemical characteristics as well as 16S rRNA analysis. Seven LAB strains were isolated from elephant grass silage. Three strains (Pediococcus acidilactici (AZZ1), Lactobacillus plantarum subsp...
October 4, 2018: Journal of Applied Microbiology
Kun Zhang, Polly Wells, Yi Liang, John Love, David A Parker, Carolina Botella
Yeast propagation using 50% diluted hydrolysate in water was utilized for the fermentation of hydrolysate derived from pre-treated ensiled sweet sorghum. The purpose was to condition the yeast to the inhibitors generated during the ensiling of sweet sorghum. The conditioned seed cultures exhibited similar fermentation performance and superior kinetics than the inoculum prepared in YPD medium. Furthermore, the conditioned yeast showed increased tolerance to the increased levels of these inhibitors, including ethanol, acetic and lactic acids, demonstrating an effective way to increase the robustness of yeast fermentation for ethanol production...
September 17, 2018: Bioresource Technology
Wencan Ke, Wurong Ding, Dongmei Xu, Mian Nazir Shah, Ping Zhang, Xusheng Guo
The aim of this study was to evaluate the effect of two organic acids [malic acid (MA) and citric acid (CA)], Lactobacillus plantarum (LP) and their mixtures on the fermentation of alfalfa silage. The wilted and chopped alfalfa was ensiled in vacuum-sealed plastic bags (200-230 g per bag) with following additives: 0.5% distilled water (control), 0.5% DL-MA, 0.5% CA, LP (1 · 106  cfu/g), 0.5% DL-MA plus LP (MA+LP) or 0.5% CA plus LP (CA+LP) (application rates based on fresh weight). Each treatment had four replicates and was stored at room temperature...
December 2018: Archives of Animal Nutrition
D M Xu, W C Ke, P Zhang, F H Li, X S Guo
AIMS: Characteristics of a strain Pediococcus pentosaceus Q6 isolated from Elymus nutans growing on the Tibetan plateau and its effects on Elymus nutans silage fermentation stored at low temperature were investigated. METHODS AND RESULTS: Sugar fermentation pattern and growth profiles of the strain Q6 and its reference strain APP were characterized. The strain Q6 and APP were inoculated to Elymus nutans at ensiling, respectively; and ensiled at different temperatures (10, 15 and 25°C) for 30, 60 and 90 days...
September 17, 2018: Journal of Applied Microbiology
Siran Wang, Zhihao Dong, Junfeng Li, Lei Chen, Tao Shao
AIMS: To examine five lactic acid bacteria (LAB) strains (LCG9, LTG7, I5, TG1 and LI3) isolated from the Tibetan Plateau, and evaluate their combined effects on the silage quality of Italian ryegrass (Lolium multiflorum Lam.) at three temperatures (10°C, 15°C and 25°C). METHODS AND RESULTS: The isolated strains were evaluated by morphological, physiological and biochemical tests. Six combined inoculants LCG9+LI3, LCG9+I5, LCG9+TG1, LTG7+LI3, LTG7+I5 and LTG7+TG1 were added to Italian ryegrass for ensiling 30 days in laboratory silos (1L) at various temperatures, respectively...
August 22, 2018: Journal of Applied Microbiology
Yi Wang, Cheng Wang, Wei Zhou, Fu-Yu Yang, Xiao-Yang Chen, Qing Zhang
The objective of this study was to evaluate the effects of wilting and Lactobacillus plantarum (LP) addition on the silage fermentation quality and microbial community of Moringa oleifera Lam. leaf silage. Unwilted (direct-cut) or wilted M. oleifera leaves were prepared either with or without LP (1.0 × 106 cfu/g) followed by either 60 or 120 days of ensiling, leading to eight treatment groups. The results showed that lactic acid was the dominant fermentation product, and no butyric acid was detected for any of the treatments...
2018: Frontiers in Microbiology
Liwen He, Wei Zhou, Yi Wang, Cheng Wang, Xiaoyang Chen, Qing Zhang
This study was designed to evaluate the effect of applying lactic acid bacteria (LAB, Lactobacillus plantarum LP) (0 and 1 × 106  cfu/g fresh matter (FM)) and cellulase (0, 30, 60 and 120 U/g FM) on the ensiling characteristics of N. cadamba leaves. The experimental design was a completely randomized 2 × 4 factorial arrangement. Fermentation parameters, microbial populations, chemical compositions and in vitro digestibility of the silages were measured after 60-day fermentation. LAB inoculation decreased the pH (p < 0...
July 30, 2018: Journal of Animal Physiology and Animal Nutrition
Dongxia Li, Kuikui Ni, Yingchao Zhang, Yanli Lin, Fuyu Yang
Objective: In tropical regions, as in temperate regions where seasonality of forage production occurs, well-preserved forage is necessary for animal production during periods of forage shortage. However, the unique climate conditions (hot and humid) and forage characteristics (high moisture content and low soluble carbohydrate) in the tropics make forage preservation more difficult. The current study used natural ensiling of tropical forage as a model to evaluate silage characteristics under different temperatures (28°C and 40°C)...
July 26, 2018: Asian-Australasian Journal of Animal Sciences
Young Ho Joo, Dong Hyeon Kim, Dimas H V Paradhipta, Hyuk Jun Lee, Sardar M Amanullah, Sang Bum Kim, Jong Soo Chang, Sam Churl Kim
Objective: This study was conducted to evaluate the effect of homo or hetero fermentative inoculants on fermentation quality and aerobic stability of sweet potato vine (SPV) silage containing Italian ryegrass hay as moisture absorbent. Methods: The SPV was harvested at 15% DM, mixed with Italian ryegrass hay at 1:1 ratio on a fresh weight basis, and chopped to 3-5 cm length. After then, the chopped forage mixture was ensiled into 20-L mini silo in quadruplicate for 7, 48 and 100 days after application of microbial inoculants at 1...
July 26, 2018: Asian-Australasian Journal of Animal Sciences
Seyed Mohsen Hosseini, Mohsen Danesh Mesgaran, Ali Reza Vakili, Abbas Ali Naserian, Ehsan Khafipour
Objective: We hypothesized that silage additives may alter the undigested NDF (uNDF) content through ensiling. Therefore, urea and formic acid applied to corn, whole barley crop (WBC) and alfalfa in order to change uNDF content of the ensiled forages. Methods: Six experimental diets at two groups of high uNDF (untreated corn and alfalfa silages (CSAS) and untreated whole barley and alfalfa silages (BSAS)) and low uNDF (urea-treated corn silage + untreated alfalfa silage (CSUAS), urea-treated whole barley silage + untreated alfalfa silage (BSUAS), untreated corn silage + formic acid-treated alfalfa silage (CSASF) and untreated whole barley silage + formic acid-treated alfalfa silage (BSASF)), were allocated to thirty-six multiparous lactating Holstein dairy cows...
July 26, 2018: Asian-Australasian Journal of Animal Sciences
Xujiao Li, Jipeng Tian, Qing Zhang, Yun Jiang, Zhe Wu, Zhu Yu
This study was conducted to study the effects of ensiled alfalfa (Medicago sativa) and red clover (Trifolium pretense) at different ratios on dynamics of fermentation parameters, N distribution, protein fractions, and protease activities during ensiling. Alfalfa and red clover were harvested and wilted to 35 and 25% dry matter, respectively, chopped to 1 cm, mixed, weighed into 1.0-L buckets at a density of 700 g/L, and ensiled for 1, 3, 7, 15, and 30 d at 30°C. The treatments were mixing ratio of alfalfa to red clover at 100:0, 70:30, 50:50, 30:70, and 0:100 (R0, R30, R50, R70, and R100, respectively; fresh weight)...
July 18, 2018: Journal of Dairy Science
Qinhua Liu, Steven E Lindow, Jianguo Zhang
Enhancing the aerobic stability of silage is very important in silage production. The objective of this study was to compare the roles of a new bacterial strain Lactobacillus parafarraginis ZH1 with Lactobacillus plantarum Chikuso-1 (LP) and Lactobacillus buchneri NCIMB 40788 (LB) in improving the aerobic stability of oat silage and identify the anti-yeast substances produced by them. After ensiled for 45 days, either inoculated silages or control silage were of pH values lower than 4.2. The control and LP inoculated silage had poorer aerobic stability than LB and ZH1 inoculated ones (p < 0...
September 2018: Animal Science Journal, Nihon Chikusan Gakkaihō
U Tayyab, R G Wilkinson, G L Charlton, C K Reynolds, L A Sinclair
The particle size of the forage has been proposed as a key factor to ensure a healthy rumen function and maintain dairy cow performance, but little work has been conducted on ryegrass silage (GS). To determine the effect of chop length of GS and GS:maize silage (MS) ratio on the performance, reticular pH, metabolism and eating behaviour of dairy cows, 16 multiparous Holstein-Friesian cows were used in a 4×4 Latin square design with four periods each of 28-days duration. Ryegrass was harvested and ensiled at two mean chop lengths (short and long) and included at two ratios of GS:MS (100:0 or 40:60 dry matter (DM) basis)...
July 9, 2018: Animal: An International Journal of Animal Bioscience
Jie Zhao, Zhihao Dong, Junfeng Li, Lei Chen, Yunfeng Bai, Yushan Jia, Tao Shao
The fermentation characteristics, structural carbohydrate degradation and enzymatic hydrolysis of rice straw ensiled with hemicellulase and Lactobacillus plantarum were examined. Fresh rice straw was ensiled in 1-L laboratory silos with no additive control (CK), L. plantarum (L), hemicellulase (HC) and hemicellulase + L. plantarum (HCL) for 6, 15, 30 and 60 days. All additives increased lactic acid concentration, and reduced pH and lignocellulosic content of the resulting silage relative to the control...
October 2018: Bioresource Technology
M Abbasi, Y Rouzbehan, J Rezaei, S E Jacobsen
This study assessed the influence of wilting, lactobacillus (LAB) and / or molasses on the chemical composition, phenolic compounds, in situ degradability and in vitro ruminal fermentation parameters of amaranth (var. Maria) silage using a randomized complete block design with 6 replicates. Treatments were fresh amaranth forage (FAF), ensiled amaranth without additive (EA), EA inoculated with LAB (EAB), EA + 5% of molasses (EAM), EA inoculated with LAB + 5% of molasses (EABM) and 24-h wilted EA (WEA). The ensiled materials were stored anaerobically for a period of 45 d...
June 25, 2018: Journal of Animal Science
J R Gandra, C S Takiya, T A Del Valle, E R Oliveira, R H T B de Goes, E R S Gandra, J D O Batista, H M C Araki
Chitosan is a biopolymer derived from chitin deacetylation, present in the exoskeleton of crustaceans and insects. Chitosan has been evaluated as rumen modulator and silage additive due to its antimicrobial properties. The objective of this study was to determine the effects of both chitosan and a bacterial additive on microbiological quality, chemical composition, nutrient in vitro degradation, fermentative profile, and total losses of whole-soybean plant silage (SS) harvested at R6 stage. Four treatments in a factorial arrangement were randomly assigned to 40 experimental minisilos as no additives (CON), 8 g/t fresh forage of microbial inoculant (INO; Kera SIL, Kera Nutrição Animal, Bento Gonçalves, Brazil); 5 g/kg of fresh forage chitosan (CHI); and CHI + INO...
September 2018: Journal of Dairy Science
Eric Lim Teik Chung, Michael Predith, Frisco Nobilly, Anjas Asmara Samsudin, Faez Firdaus Abdullah Jesse, Teck Chwen Loh
Brachiaria decumbens is an extremely productive tropical grass due to its aggressive growth habit and its adaptation to a varied range of soil types and environments. As a result of the vast availability, treated B. decumbens demonstrates as a promising local material that could be utilised as an improved diet for sheep and goats. Despite the fact that the grass significantly increases weight gains in grazing farm animals, there were many reports of general ill-thrift and sporadic outbreaks of photosensitivity in livestock due to the toxic compound of steroidal saponin found in B...
June 20, 2018: Tropical Animal Health and Production
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