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Seong Shin Lee, Hyuk Jun Lee, Dimas Hand Vidya Paradhipta, Young Ho Joo, Sang Bum Kim, Dong Hyeon Kim, Sam Churl Kim
Objectve: This study was conducted to estimate the temperature and microbial changes of corn silages during aerobic exposure. Methods: Kwangpyeongok (KW) and Pioneer 1543 (PI) corn hybrids were harvested at 29.7% of dry matter and chopped to 3-5 cm lengths. Homo (Lactobacillus plantarum; LP) or hetero (L. buchneri; LB) fermentative inoculants at 1.2 × 105 cfu/g of fresh forage was applied to the chopped corn forage which was then ensiled in quadruplicate with a 2 x 2 (hybrid × inoculant) treatment arrangement for 100 days...
November 27, 2018: Asian-Australasian Journal of Animal Sciences
Jie Zhao, Zhihao Dong, Junfeng Li, Lei Chen, Yunfeng Bai, Yushan Jia, Tao Shao
Objectve: This study was to evaluate the fermentation dynamics, structural and nonstructural carbohydrate composition and in vitro gas production of rice straw ensiled with lactic acid bacteria (LAB) and molasses. Methods: Fresh rice straw was ensiled in 1-L laboratory silos with no additive control (C), Lactobacillus plantarum (L), molasses (M) and molasses + L. plantarum (ML) for 6, 15, 30 and 60 days. After storage, the silages were subjected to microbial and chemical analyses as well as the further in vitro fermentation trial...
November 27, 2018: Asian-Australasian Journal of Animal Sciences
Lin Sun, Zhijun Wang, Ge Gentu, Yushan Jia, Meiling Hou, Yimin Cai
Objectve: To effectively use corn stover as animal feed, we studied the changes in microbial population and chemical composition of three cultivar corn stover during field exposure and their effects on silage fermentation and in vitro digestibility. Methods: The corn stover silages were prepared from stovers exposed in the field for 0, 7, 15, 30, 60, 90, and 180 d. The microbial composition, chemical composition, fermentation quality, and in vitro digestibility were analyzed...
November 27, 2018: Asian-Australasian Journal of Animal Sciences
Beiyi Liu, Hailin Huan, Hongru Gu, Nengxiang Xu, Qin Shen, Chenlong Ding
This study investigated the effects of lactic acid bacteria on bacterial and fungal community during the fermentation process and aerobic exposure phase of barley ensiled with preparation of lactic acid bacteria (LAB). The inoculated silages displayed higher contents of lactic acid, acetic acid, and propionic acid as well as a greater number of lactic acid bacteria during ensiling. LAB-treated silage decreased the bacterial diversity during both ensiling and aerobic exposure but increased the fungal diversity during ensiling of barley...
October 22, 2018: Bioresource Technology
Mariele Cristina Nascimento Agarussi, Odilon Gomes Pereira, Vanessa Paula da Silva, Eliana Santos Leandro, Karina Guimarães Ribeiro, Stefanie Alvarenga Santos
This study was conducted to evaluate the fermentative profile and microbial populations of wilted and non-wilted alfalfa silages ensiled with or without inoculant and the population dynamics of lactic acid bacteria (LAB) of wilted alfalfa plant and theirs silage. A 2 × 2 × 6 factorial arrangement was used, with the absence or presence of wilting (W), with and without bacterial inoculant (I) and six fermentation periods (P) (1, 3, 7, 14, 28 and 56 days), in a completely randomized design, with three replicates...
November 15, 2018: Molecular Biology Reports
Milad Hadidi, Albert Ibarz, Josep Conde, Jordi Pagan
The use of alfalfa protein in human food is limited by its low quality. Response Surface Methodology was employed to optimise the combined effects of different steam blanching conditions on the enzymatic activity, browning and protein degrading which cause undesirable characteristics. The optimum conditions were: steaming time 4.36 min, particle size 23 mm, time from harvesting to steaming 2 h leading to a residual activity of polyphenol oxidase of 1.31% and a completely inactivation of peroxidase. The Browning Index value was 108...
March 15, 2019: Food Chemistry
Rebeca Castro, Isabel Guerrero-Legarreta, Rodrigo Bórquez
Chitin extraction from Allopetrolisthes punctatus , a crab species proliferating in Chile and Peru seashores, was carried out applying preliminary lactic ensilation. For this purpose, Lactobacillus plantarum sp. 47 isolated from Coho salmon was inoculated in crab biomass. Previously, fermentation parameters (carbon source, inoculum concentration and incubation temperature) to obtain peak lactic acid production and bacterial growth were studied. The optimal fermentation conditions were 10% inoculum, 15% sucrose and 85% crab biomass, producing 17 mg lactic acid/ g silage...
December 2018: Biotechnology Reports
Siran Wang, Zhihao Dong, Junfeng Li, Lei Chen, Tao Shao
AIMS: To examine the characteristics of three isolated Pediococcus acidilactici strains (LTG7, LOG9 and LH9) and evaluate their effects on silage quality, nutritive value and in vitro ruminal digestibility in a variety of forages. METHODS AND RESULTS: One commercial inoculant Lactobacillus plantarum MTD-1 (G) and three isolated lactic acid bacteria (LAB) strains were measured by morphological, physiological and biochemical tests. All the LAB strains were added to Italian ryegrass (Lolium multiflorum Lam...
November 1, 2018: Journal of Applied Microbiology
Snehasish Mishra, Puneet Kumar Singh, Swagatika Dash, Ritesh Pattnaik
Biogas obtained from organic remains entails a developed technology and an appreciable methane yield, but its use may not be sustainable. The potential methane yield of various lignocellulose biomass and the operational conditions employed are inherently reviewed. Although of lower methane yields compared to conventional substrates, agricultural biomass is a cheap option. The major challenges encountered during its biogasification are its recalcitrance nature primarily due to the presence of crystalline cellulose and lignin...
November 2018: 3 Biotech
Eoin Byrne, Krisztina Kovacs, Ed W J van Niel, Karin Willquist, Sven-Erik Svensson, Emma Kreuger
Background: Current EU directives demand increased use of renewable fuels in the transportation sector but restrict governmental support for production of biofuels produced from crops. The use of intercropped lucerne and wheat may comply with the directives. In the current study, the combination of ensiled lucerne ( Medicago sativa L.) and wheat straw as substrate for hydrogen and methane production was investigated. Steam-pretreated and enzymatically hydrolysed wheat straw [WSH, 76% of total chemical oxygen demand (COD)] and ensiled lucerne (LH, 24% of total COD) were used for sequential hydrogen production through dark fermentation and methane production through anaerobic digestion and directly for anaerobic digestion...
2018: Biotechnology for Biofuels
Malgorzata Szumacher-Strabel, Anna Stochmal, Adam Cieslak, Martyna Kozłowska, Dawid Kuznicki, Mariusz Kowalczyk, Wieslaw Oleszek
BACKGROUND: Alfalfa (Medicago sativa) is an important legume used in animal feed. The primary objective in this study was to analyze and trace the individual saponins in fresh and ensiled alfalfa of ten varieties, with the aim of evaluating the structural changes undergone by saponins during ensiling. A secondary objective was to examine whether any of the changes in saponin content were associated with changes in the basic nutrient composition of the ensiled alfalfa. RESULTS: The total saponin concentration increased when the fresh alfalfa was processed into silage...
October 16, 2018: Journal of the Science of Food and Agriculture
Lei Chen, Zhihao Dong, Junfeng Li, Tao Shao
BACKGROUND: Silages produced with grass and legume mixtures may have positive associative effects on silage quality, and animal performance when ruminants are fed the silages. The objective of this study was to determine the preservation characteristics, in vitro rumen fermentation profile and microbial abundance of silages, produced with mixtures of sweet sorghum (SS) and alfalfa (AF) in different ratios: 100: 0 (Control), 75: 25 (SA25), 50: 50 (SA50), 25: 75 (SA75) and 0: 100 (SA100) on a fresh weight basis...
October 9, 2018: Journal of the Science of Food and Agriculture
Azizza Sifeeldein, Siran Wang, Junfeng Li, Zhihao Dong, Lei Chen, Niaz Ali Kaka, Tao Shao
AIM: to isolate, screen and identify the lactic acid bacteria (LAB) from elephant silage during the process of the fermentation and their effects on the fermentation quality of sweet sorghum silage. METHODS AND RESULTS: The isolated strains were identified based on morphological, physiological and biochemical characteristics as well as 16S rRNA analysis. Seven LAB strains were isolated from elephant grass silage. Three strains (Pediococcus acidilactici (AZZ1), Lactobacillus plantarum subsp...
October 4, 2018: Journal of Applied Microbiology
Kun Zhang, Polly Wells, Yi Liang, John Love, David A Parker, Carolina Botella
Yeast propagation using 50% diluted hydrolysate in water was utilized for the fermentation of hydrolysate derived from pre-treated ensiled sweet sorghum. The purpose was to condition the yeast to the inhibitors generated during the ensiling of sweet sorghum. The conditioned seed cultures exhibited similar fermentation performance and superior kinetics than the inoculum prepared in YPD medium. Furthermore, the conditioned yeast showed increased tolerance to the increased levels of these inhibitors, including ethanol, acetic and lactic acids, demonstrating an effective way to increase the robustness of yeast fermentation for ethanol production...
January 2019: Bioresource Technology
Wencan Ke, Wurong Ding, Dongmei Xu, Mian Nazir Shah, Ping Zhang, Xusheng Guo
The aim of this study was to evaluate the effect of two organic acids [malic acid (MA) and citric acid (CA)], Lactobacillus plantarum (LP) and their mixtures on the fermentation of alfalfa silage. The wilted and chopped alfalfa was ensiled in vacuum-sealed plastic bags (200-230 g per bag) with following additives: 0.5% distilled water (control), 0.5% DL-MA, 0.5% CA, LP (1 · 106  cfu/g), 0.5% DL-MA plus LP (MA+LP) or 0.5% CA plus LP (CA+LP) (application rates based on fresh weight). Each treatment had four replicates and was stored at room temperature...
December 2018: Archives of Animal Nutrition
D M Xu, W C Ke, P Zhang, F H Li, X S Guo
AIMS: Characteristics of a strain Pediococcus pentosaceus Q6 isolated from Elymus nutans growing on the Tibetan plateau and its effects on E. nutans silage fermentation stored at low temperature were investigated. METHODS AND RESULTS: Sugar fermentation pattern and growth profiles of the strain Q6 and its reference strain APP were characterized. The strain Q6 and APP were inoculated to E. nutans at ensiling respectively; and ensiled at different temperatures (10, 15 and 25°C) for 30, 60 and 90 days...
September 17, 2018: Journal of Applied Microbiology
Siran Wang, Zhihao Dong, Junfeng Li, Lei Chen, Tao Shao
AIMS: To examine five lactic acid bacteria (LAB) strains (LCG9, LTG7, I5, TG1 and LI3) isolated from the Tibetan Plateau, and evaluate their combined effects on the silage quality of Italian ryegrass (Lolium multiflorum Lam.) at three temperatures (10°C, 15°C and 25°C). METHODS AND RESULTS: The isolated strains were evaluated by morphological, physiological and biochemical tests. Six combined inoculants LCG9+LI3, LCG9+I5, LCG9+TG1, LTG7+LI3, LTG7+I5 and LTG7+TG1 were added to Italian ryegrass for ensiling 30 days in laboratory silos (1L) at various temperatures, respectively...
August 22, 2018: Journal of Applied Microbiology
Yi Wang, Cheng Wang, Wei Zhou, Fu-Yu Yang, Xiao-Yang Chen, Qing Zhang
The objective of this study was to evaluate the effects of wilting and Lactobacillus plantarum (LP) addition on the silage fermentation quality and microbial community of Moringa oleifera Lam. leaf silage. Unwilted (direct-cut) or wilted M. oleifera leaves were prepared either with or without LP (1.0 × 106 cfu/g) followed by either 60 or 120 days of ensiling, leading to eight treatment groups. The results showed that lactic acid was the dominant fermentation product, and no butyric acid was detected for any of the treatments...
2018: Frontiers in Microbiology
Liwen He, Wei Zhou, Yi Wang, Cheng Wang, Xiaoyang Chen, Qing Zhang
This study was designed to evaluate the effect of applying lactic acid bacteria (LAB, Lactobacillus plantarum LP) (0 and 1 × 106  cfu/g fresh matter (FM)) and cellulase (0, 30, 60 and 120 U/g FM) on the ensiling characteristics of N. cadamba leaves. The experimental design was a completely randomized 2 × 4 factorial arrangement. Fermentation parameters, microbial populations, chemical compositions and in vitro digestibility of the silages were measured after 60-day fermentation. LAB inoculation decreased the pH (p < 0...
July 30, 2018: Journal of Animal Physiology and Animal Nutrition
Dongxia Li, Kuikui Ni, Yingchao Zhang, Yanli Lin, Fuyu Yang
Objective: In tropical regions, as in temperate regions where seasonality of forage production occurs, well-preserved forage is necessary for animal production during periods of forage shortage. However, the unique climate conditions (hot and humid) and forage characteristics (high moisture content and low soluble carbohydrate) in the tropics make forage preservation more difficult. The current study used natural ensiling of tropical forage as a model to evaluate silage characteristics under different temperatures (28°C and 40°C)...
July 26, 2018: Asian-Australasian Journal of Animal Sciences
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