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High iron levels

Christopher J Greene, Nitika J Sharma, Peter N Fiorica, Emily Forrester, Gary J Smith, Kenneth W Gross, Eric C Kauffman
BACKGROUND AND OBJECTIVES: Accumulating data implicate iron accumulation in tumorigenesis of the kidney, particularly the clear cell renal cell carcinoma (ccRCC) subtype. The von Hippel Lindau (VHL)/hypoxia inducible factor-α (HIF-α) axis is uniquely dysregulated in ccRCC and is a major regulator and regulatory target of iron metabolism, yet the role of iron in ccRCC tumorigenesis and its potential interplay with VHL inactivation remains unclear. We investigated whether ccRCC iron accumulation occurs due to increased cell dependency on iron for growth and survival as a result of VHL inactivation...
December 13, 2018: Free Radical Biology & Medicine
Javiera Ramos-Zúñiga, Sebastian Gallardo, Cristóbal Martínez-Bussenius, Rodrigo Norambuena, Claudio A Navarro, Alberto Paradela, Carlos A Jerez
Cadmium is a heavy metal present in contaminated soils. It has no biological role but when entering cells generates DNA damage, overexpression of stress response proteins and misfolded proteins, amongst other deleterious effects. Acidithiobacillus ferrooxidans is an acidophilic bacterium resisting high concentrations of heavy metals such as cadmium. This is important for industrial bioleaching processes where Cd+2 concentrations can be 5-100 mM. Cadmium resistance mechanisms in these microorganisms have not been fully characterized...
December 13, 2018: Journal of Proteomics
Yin Yee Leong, Wai Hoe Ng, Mimi Zulaikha Umar Fuaad, Chin Theng Ng, Rajesh Ramasamy, Vuanghao Lim, Yoke Keong Yong, Jun Jie Tan
Stem cell therapy offers hope to reconstitute injured myocardium and salvage heart from failing. A recent approach using combinations of derived Cardiac-derived c-kit expressing cells (CCs) and mesenchymal stem cells (MSCs) in transplantation improved infarcted hearts with a greater functional outcome, but the effects of MSCs on CCs remain to be elucidated. We used a novel two-step protocol to clonogenically amplify colony forming c-kit expressing cells from 4- to 6-week-old C57BL/6N mice. This method yielded highly proliferative and clonogenic CCs with an average population doubling time of 17...
December 11, 2018: Journal of Cellular Biochemistry
Chiara Volani, Giuseppe Paglia, Sigurdur V Smarason, Peter P Pramstaller, Egon Demetz, Christa Pfeifhofer-Obermair, Guenter Weiss
Iron is an essential co-factor for several metabolic processes, including the Krebs cycle and mitochondrial oxidative phosphorylation. Therefore, maintaining an appropriate iron balance is essential to ensure sufficient energy production and to avoid excessive reactive oxygen species formation. Iron overload impairs mitochondrial fitness; however, little is known about the associated metabolic changes. Here we aimed to characterize the metabolic signature triggered by dietary iron overload over time in a mouse model, where mice received either a standard or a high-iron diet...
December 11, 2018: Cells
Sarah Eskreis-Winkler, Giuseppe Corrias, Serena Monti, Junting Zheng, Marinela Capanu, Simone Krebs, Maggie Fung, Scott Reeder, Lorenzo Mannelli
BACKGROUND: Cancer patients often have a history of chemotherapy, putting them at increased risk of liver toxicity and pancytopenia, leading to elevated liver fat and elevated liver iron respectively. T1-in-and-out-of-phase, the conventional MR technique for liver fat assessment, fails to detect elevated liver fat in the presence of concomitantly elevated liver iron. IDEAL-IQ is a more recently introduced MR fat quantification method that corrects for multiple confounding factors, including elevated liver iron...
December 12, 2018: Cancer Imaging: the Official Publication of the International Cancer Imaging Society
Robert H Lampe, Elizabeth L Mann, Natalie R Cohen, Claire P Till, Kimberlee Thamatrakoln, Mark A Brzezinski, Kenneth W Bruland, Benjamin S Twining, Adrian Marchetti
Diatoms are prominent eukaryotic phytoplankton despite being limited by the micronutrient iron in vast expanses of the ocean. As iron inputs are often sporadic, diatoms have evolved mechanisms such as the ability to store iron that enable them to bloom when iron is resupplied and then persist when low iron levels are reinstated. Two iron storage mechanisms have been previously described: the protein ferritin and vacuolar storage. To investigate the ecological role of these mechanisms among diatoms, iron addition and removal incubations were conducted using natural phytoplankton communities from varying iron environments...
December 11, 2018: Proceedings of the National Academy of Sciences of the United States of America
Anne-Sylvia Sacri, Blandine de Lauzon-Guillain, Marie-Noëlle Dufourg, Corinne Bois, Marie Aline Charles, Martin Chalumeau
Sacri A-S, de Lauzon-Guillain B, Dufourg M-D, Bois C, Charles M A, Chalumeau M. Iron-fortified formula use in young children and association with socioeconomic factors in the French nationwide ELFE cohort. AIM: To study the rate of iron-fortified infant formula (IFF) use in young children in France and its association with socioeconomic factors. METHODS: The ELFE national birth cohort included, in 2011, 18,329 living births in 349 hospitals randomly selected. The present analyses were restricted to children with follow-up at age 2 years...
December 7, 2018: Acta Paediatrica
Sahar Trabelsi, Mouna Sahnoun, Fatma Elgharbi, Rihab Ameri, Sameh Ben Mabrouk, Monia Mezghani, Aïda Hmida-Sayari, Samir Bejar
A synthetic cDNA-AmyA gene was cloned and successfully expressed in Pichia pastoris as a His-tagged enzyme under the methanol inducible AOX1 promoter. High level of extracellular amylase production of 72 U/mL was obtained after a 72 h induction by methanol. As expected, the recombinant strain produced only the AmyA isoform since the host is a protease deficient strain. Besides, the purified r-AmyA showed a molecular mass of 54 kDa, the same pH optimum equal to 5.6 but a higher thermoactivity of 60 °C against 50 °C for the native enzyme...
December 7, 2018: Molecular Biology Reports
G K Duskaev, S G Rakhmatullin, N M Kazachkova, Y V Sheida, I N Mikolaychik, L A Morozova, B H Galiev
Aim: This study was designed to investigate the synergistic effect of the combined action of probiotic bacterial strains ( Bifidobacterium adolescentis and Lactobacillus acidophilus ) and Quercus cortex extract as biologically active substances in the feed on the immunity and productivity of Gallus gallus domesticus . Materials and Methods: For the experiment, 120 7-day-old broiler chickens were selected (4 groups, n=30, 3 replicates with 10 birds in each group)...
November 2018: Veterinary World
Kathryna Fontana Rodrigues, Nathalia Teixeira Pietrani, Laura Machado Lara Carvalho, Adriana Aparecida Bosco, Valéria Cristina Sandrim, Cláudia Natália Ferreira, Karina Braga Gomes
BACKGROUND: Type 2 diabetes mellitus (T2DM) is an inflammatory condition associated to obesity and increased oxidative stress. Haptoglobin (Hp) is an acute phase reactant that scavenges extracorpuscular hemoglobin from circulation and prevents heme-iron oxidative damage. OBJECTIVE: To assess the association between Hp levels and Hp1-Hp2 gene polymorphism and clinical and laboratory parameters in patients with T2DM. METHODS: The study sample consisted of 102 T2DM patients and 62 controls...
October 24, 2018: Endocrinología, Diabetes y Nutrición
Toshifumi Hoki, Eriko Katsuta, Li Yan, Kazuaki Takabe, Fumito Ito
BACKGROUND: Despite a high rate of recurrences, long-term survival can be achieved after the resection of hepatocellular carcinoma (HCC) with effective local treatment. Discovery of adverse prognostic variables to identify patients with high risk of recurrence could improve the management of HCC. Accumulating evidence showing a link between carcinogenesis and increased expression of iron import proteins and intracellular iron prompted us to investigate a role of divalent metal-ion transporter-1 (DMT1) that binds and regulates a variety of divalent metals in HCC...
February 2019: Journal of Surgical Research
Richard Thomas Wilkin, Tony Lee, Molly Sexton, Steven D Acree, Robert Puls, David W Blowes, Christopher Kalinowski, Jennifer Tilton, Leilani Woods
This study provides a twenty-two-year record of in situ degradation of chlorinated organic compounds by a granular iron permeable reactive barrier (PRB). Groundwater concentrations of trichloroethene (TCE) entering the PRB were as high as 10,670 µg/L. Treatment efficiency ranged from 81 to >99% and TCE concentrations from <1 µg/L to 165 µg/L were detected within and hydraulically down-gradient of the PRB. After 18 years, effluent TCE concentrations were above the maximum contaminant level (MCL) along segments of the PRB exhibiting upward trending influent TCE...
December 9, 2018: Environmental Science & Technology
Tom Murphy, Kongkea Phan, Emmanuel Yumvihoze, Kim Irvine, Ken Wilson, David Lean, Alexander Poulain, Brian Laird, Laurie Hing Man Chan
Background: In parts of Cambodia, irrigation with groundwater results in arsenic accumulation in soils and rice, leading to health concerns associated with rice consumption. A high concentration of iron in groundwater can precipitate arsenic and reduce its bioavailability, however high concentrations of arsenic and iron can also reduce rice production. Furthermore, concerns have been raised about chemical contamination from inorganic fertilizers used to grow rice. The relationship between soil geochemistry and arsenic concentrations in rice is not yet fully understood...
September 2018: Journal of health & pollution
Jizhen Wei, Shuo Yang, Lin Chen, Xiaoguang Liu, Mengfang Du, Shiheng An, Gemei Liang
Helicoverpa armigera can develop resistance to Bacillus thuringiensis (Bt), which threaten the long-term success of Bt crops. In the present study, RNAseq was employed to investigate the midgut genes response to strains with different levels of resistance (LF5, LF10, LF20, LF30, LF60, and LF120) in H. armigera . Results revealed that a series of differentially expressed unigenes (DEGs) were expressed significantly in resistant strains compared with the LF-susceptible strain. Nine trypsin genes, ALP2 , were downregulated significantly in all the six resistant strains and further verified by qRT-PCR, indicating that these genes may be used as markers to monitor and manage pest resistance in transgenic crops...
2018: Frontiers in Physiology
Rongyan Chen, Nan Lin Wang
The presence of magnetic ions was believed to be detrimental to superconductivity in early stage. However, unconventional superconductivity was widely induced by doping or applying external pressure in magnetic systems such as heavy fermion, cuprate and iron-based superconductors, in which magnetic fluctuations are suggested to serve as the pairing glue for Cooper pairs.&#13; The discoveries of superconductivity in the magnetic compounds CrAs and MnP under high pressures have further enriched this family and provided new platforms to investigate the interplay between magnetism and superconductivity...
October 31, 2018: Reports on Progress in Physics
Anna Edlund, Youngik Yang, Shibu Yooseph, Xuesong He, Wenyuan Shi, Jeffrey S McLean
BACKGROUND: Dental plaque is composed of hundreds of bacterial taxonomic units and represents one of the most diverse and stable microbial ecosystems associated with the human body. Taxonomic composition and functional capacity of mature plaque is gradually shaped during several stages of community assembly via processes such as co-aggregation, competition for space and resources, and by bacterially produced reactive agents. Knowledge on the dynamics of assembly within complex communities is very limited and derives mainly from studies composed of a limited number of bacterial species...
December 6, 2018: Microbiome
Karen S Romanello, Karina K L Teixeira, João Pedro M O Silva, Sheila T Nagamatsu, Marcos André C Bezerra, Igor F Domingos, Diego A P Martins, Aderson S Araujo, Carolina Lanaro, Carlos A Breyer, Regiane A Ferreira, Carla Franco-Penteado, Fernando F Costa, Iran Malavazi, Luis E S Netto, Marcos A de Oliveira, Anderson F Cunha
β-thalassemia is a worldwide distributed monogenic red cell disorder, characterized by an absent or reduced beta globin chain synthesis. The unbalance of alpha-gamma chain and the presence of pathological free iron promote severe oxidative damage, playing crucial a role in erythrocyte hemolysis, exacerbating ineffective erythropoiesis and decreasing the lifespan of red blood cells (RBC). Catalase, glutathione peroxidase and peroxiredoxins act together to protect RBCs from hydrogen peroxide insult. Among them, peroxiredoxins stand out for their overall abundance and reactivity...
2018: PloS One
Michael Schorsch, Manuela Kramer, Tatjana Goss, Marion Eisenhut, Nigel Robinson, Deenah Osman, Annegret Wilde, Shamaila Sadaf, Hendrik Brückler, Lorenz Walder, Renate Scheibe, Toshiharu Hase, Guy T Hanke
Iron chronically limits aquatic photosynthesis, especially in marine environments, and the correct perception and maintenance of iron homeostasis in photosynthetic bacteria, including cyanobacteria, is therefore of global significance. Multiple adaptive mechanisms, responsive promoters, and posttranscriptional regulators have been identified, which allow cyanobacteria to respond to changing iron concentrations. However, many factors remain unclear, in particular, how iron status is perceived within the cell...
December 4, 2018: Proceedings of the National Academy of Sciences of the United States of America
Yedidyah Weiss, Brenden Chen, Makiko Yasuda, Irina Nazarenko, Karl E Anderson, Robert J Desnick
Porphyria Cutanea Tarda (PCT) is a cutaneous porphyria that results from the hepatic inhibition of the heme biosynthetic enzyme uroporphyrinogen decarboxylase (UROD), and can occur either in the absence or presence of an inherited heterozygous UROD mutation (PCT subtypes 1 and 2, respectively). A heterozygous UROD mutation causes half-normal levels of UROD activity systemically, which is a susceptibility factor but is not sufficient alone to cause type 2 PCT. In both Types 1 and 2 PCT, the cutaneous manifestations are precipitated by additional factors that lead to generation of an inhibitor that more profoundly reduces hepatic UROD activity...
November 28, 2018: Molecular Genetics and Metabolism
Tasuku Hirayama
Intracellular labile iron is an iron species which is not or weakly bound to proteins and depicts an important effect on homeostatic regulation in cells. An excess or deficiency of iron can cause oxidative damage to key cellular biomolecules. The behavior and concentrations of labile iron are difficult to monitor, but the specific redox state of the Fe ions is relevant to the physiological and pathological properties that we would like to study. We have developed a series of turn-on type fluorescent probes that are highly selective to the labile Fe(II) ions, and we have tested their applications to cellular level imaging...
October 31, 2018: Acta Histochemica et Cytochemica
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