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Aunaly Palmer, Lisa A Taitsman, May J Reed, Bala G Nair, Itay Bentov
Hip fractures result in significant morbidity and mortality in elders. Indicators of frailty are associated with poor outcomes. Commonly used frailty tools rely on motor skills that cannot be performed by this population. We determined the association between the Charlson Comorbidity Score (CCS), intraoperative hypotension (IOH), and a geriatric medicine consult index (GCI) with short-term mortality in hip fracture patients. A retrospective cohort study was conducted at a single institution over a 2-year period...
2018: Geriatric Orthopaedic Surgery & Rehabilitation
Ali Cevat Kutluk, Hasan Akin, Altan Ceritoglu, Celalettin Ibrahim Kocaturk, Salih Bilen, Yasar Sonmezoglu, Kemal Karapinar
PURPOSE: Lung cancer is one of the major sources of mortality in the elderly. This study was undertaken to assess the early and long-term results of surgical resection in patients older than 70 years of age by comparing the results of patients aged 70-79 years (group 1) with patients older than 80 years of age (group 2). METHODS: Data on patient age, gender, spirometry values, side, size, histology and stage of the tumor, surgical procedures, postoperative complications, Charlson comorbidity scores (CCS), and survival were collected...
December 11, 2018: Annals of Thoracic and Cardiovascular Surgery
Isabele Picada Emanuelli, Camila Bortoliero Costa, Luciana Simões Rafagnin Marinho, Marcelo Marcondes Seneda, Flávio Vieira Meirelles
This study investigated the interactions between cumulus cells (CCs) and oocytes and programmed cell death in bovine cumulus-oocyte complexes (COCs) with different morphological characteristics. DNA fragmentation was assessed in CCs at 0 and 24 h of maturation, as well as parthenogenetic developmental competence on the 9th day post-activation, blastocyst quality and BCL-2 and BAX transcript levels in matured CCs. Most immature oocytes in the COC-A group (full cumulus and several compact layers) were in the initial germinal vesicle (iGV) stage, exhibiting minimal or no DNA damage...
November 26, 2018: Theriogenology
César Morcillo Serra, Yolanda López-Perna, Antonio Monleón Getino, Elena Sánchez-Vizcaíno, Jordi Delàs Amat, Josep Masip Utset
BACKGROUND: Coronary calcium score (CCS) and coronary computed tomography angiography (CTA) assessments using multidetector computed tomography are invaluable for atheromatosis screening. We studied their usefulness in cardiovascular risk assessments, and compared evaluations using the Systematic COronary Risk Evaluation (SCORE) algorithm with those from CTA and CSS assessments in terms of their ability to predict cardiovascular events in Mediterranean patients. PATIENTS AND METHODS: Two hundred and sixty-six asymptomatic patients whose mean age was 55...
December 10, 2018: Coronary Artery Disease
Scott J Morin, Xin Tao, Diego Marin, Yiping Zhan, Jessica Landis, Jenna Bedard, Richard T Scott, Emre Seli
An algorithm assessing the methylation levels of 353 informative CpG sites in the human genome permits accurate prediction of the chronologic age of a subject. Interestingly, when there is discrepancy between the predicted age and chronologic age (age acceleration or "AgeAccel"), patients are at risk for morbidity and mortality. Identification of infertile patients at risk for accelerated reproductive senescence may permit preventative action. This study aimed to assess the accuracy of the "epigenetic clock" concept in reproductive age women undergoing fertility treatment by applying the age prediction algorithm in peripheral (white blood cells [WBCs]) and follicular somatic cells (cumulus cells [CCs]), and to identify whether women with premature reproductive aging (diminished ovarian reserve) were at risk of AgeAccel in their age prediction...
December 8, 2018: Aging
Tabbi Wilberforce, Ahmad Baroutaji, Bassel Soudan, Abdul Hai Al-Alami, Abdul Ghani Olabi
The greenhouse gases emissions produced by industry and power plants are the cause of climate change. An effective approach for limiting the impact of such emissions is adopting modern Carbon Capture and Storage (CCS) technology that can capture more than 90% of carbon dioxide (CO2 ) generated from power plants. This paper presents an evaluation of state-of-the-art technologies used in the capturing CO2 . The main capturing strategies including post-combustion, pre-combustion, and oxy - combustion are reviewed and compared...
December 4, 2018: Science of the Total Environment
Cyrielle Crampe-Casnabet, Jeanna-Eve Franck, Virginie Ringa, Mireille Coeuret-Pellicer, Pierre Chauvin, Gwenn Menvielle
BACKGROUND: Immigrant women often have lower cervical cancer screening (CCS) rates, tend to have a higher body mass index (BMI) and may be more vulnerable to BMI-related stigmatization. Our aim was to assess the role of BMI in differences in CCS rates by migration history. METHODS: Analyses were based on the 2012-2015 inclusion data (n = 27,226) for the population-based CONSTANCES cohort, including detailed, self-reported information on demographics and socioeconomic characteristics, migration history, health behaviours, health, and health care use...
December 6, 2018: Cancer Epidemiology
Atcharaporn Kidchana, Nattakan Meekhanon, Rujirat Hatrongjit, Marcelo Gottschalk, Anusak Kerdsin
Random amplification of polymorphic DNA (RAPD) and 16S-23S rDNA intergenic spacer polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were applied and evaluated to determine clonal complexes (CCs) of 684 Streptococcus suis isolates from pigs and humans. RAPD better distinguished major S. suis CCs than the PCR-RFLP method. The assay was capable of simultaneously distinguishing CC1, CC16, CC25, CC28, CC104, CC221/234, and CC233/379. PCR-RFLP could not clearly differentiate among most CCs in this study except CC16...
December 5, 2018: Molecular and Cellular Probes
Rochelle Bernier, Mohammed Al-Shehri, Satish R Raj, Lucy Reyes, Evan Lockwood, Sajad Gulamhusein, Randall Williams, Lucas Valtuille, Soori Sivakumaran, Tomasz Hruczkowski, Shane Kimber, Derek V Exner, Roopinder K Sandhu
Studies evaluating physician adherence to guideline recommendations for implantable cardioverter defibrillator (ICD) therapy are sparse, and none exist for the application of appropriate-use criteria (AUC) in clinical practice. As part of a quality improvement initiative, a review of all ICD procedures was performed from January 1, 2015 to December 31, 2016 in Alberta, Canada, to evaluate the proportion of patients receiving appropriate ICD therapy and to identify reasons for nonadherence. Our device-implant process involves an electrophysiologist or implanting cardiologist evaluation, reminders of ICD eligibility criteria on the device requisition, and peer-review consensus...
December 2018: Canadian Journal of Cardiology
Bernard Beall, Sopio Chochua, Robert E Gertz, Yuan Li, Zhongya Li, Lesley McGee, Benjamin J Metcalf, Jessica Ricaldi, Theresa Tran, Hollis Walker, Tamara Pilishvili
Invasive pneumococcal disease (IPD) has greatly decreased since implementation in the U.S. of the 7 valent conjugate vaccine (PCV7) in 2000 and 13 valent conjugate vaccine (PCV13) in 2010. We used whole genome sequencing (WGS) to predict phenotypic traits (serotypes, antimicrobial phenotypes, and pilus determinants) and determine multilocus genotypes from 5334 isolates (~90% of cases) recovered during 2015-2016 through Active Bacterial Core surveillance. We identified 44 serotypes; 26 accounted for 98% of the isolates...
2018: Frontiers in Microbiology
Jean R N Haler, Philippe Massonnet, Johann Far, Victor R de la Rosa, Philippe Lecomte, Richard Hoogenboom, Christine Jérôme, Edwin De Pauw
Polymer characterizations are often performed using mass spectrometry (MS). Aside from MS and different tandem MS (MS/MS) techniques, ion mobility-mass spectrometry (IM-MS) has been recently added to the inventory of characterization technique. However, only few studies have focused on the reproducibility and robustness of polymer IM-MS analyses. Here, we perform collisional and electron-mediated activation of polymer ions before measuring IM drift times, collision cross-sections (CCS), or reduced ion mobilities (K0 )...
December 6, 2018: Journal of the American Society for Mass Spectrometry
Kathy S Thiemann-Bourque, Nancy Brady, Lesa Hoffman
Purpose: The purpose of this study was to measure changes in communication of preschoolers with autism using the Communication Complexity Scale (CCS; Brady et al., 2012) and to examine the utility of the CCS in measuring pretreatment and posttreatment changes within peer and adult assessment contexts. Method: The CCS was used to code preassessment and postassessment for 23 children with autism randomly assigned to a treatment that incorporated a peer-mediated approach and a speech-generating device and 22 assigned to a business-as-usual condition with untrained peers...
December 6, 2018: American Journal of Speech-language Pathology
Sabrina J Ruis, Humberto Blanco-Canqui, Paul J Jasa, Richard B Ferguson, Glen Slater
Cover crops (CCs) could alter soil processes, but the effects of early versus late termination of CCs on gas fluxes are not well known. We evaluated temporal changes in fluxes of CO, NO, and CH and related soil properties in no-till corn ( L.) with and without winter rye ( L.) CCs that were terminated early (30 d before planting) or late (at planting) in a rainfed silty clay loam and an irrigated silt loam in Nebraska from April 2016 to June 2017. Gas fluxes, soil temperature, and soil water content were measured biweekly to monthly, and wet aggregate stability and particulate organic matter concentrations were measured seasonally...
November 2018: Journal of Environmental Quality
Ahmed El-Sabbagh, Medhat M Osman, Mark Fesler, Tarek Helmy, Nadeem Parker, Razi Muzaffar
This study aims to detect the potential impact of chemotherapy on the coronary calcium scoring (CCS) in lymphoma patients undergoing FDG-PET/CT at baseline and for therapy response using the CT portion of the exam to calculate the CCS. One hundred twelve lymphoma patients were included in the study based on having both baseline and at least 2 post-chemotherapy scans. The unenhanced CT portions of the scans were reviewed to measure the CCS which was then extracted using the Toshiba Vital Program. Agatston scores were assessed as category 1 with zero CCS unit and categories 2 to 5 having more than >1, 11, 101, and 400 CCS units respectively...
2018: American Journal of Nuclear Medicine and Molecular Imaging
Jacob E Porter, Prem Chapagain, Francisco Fernandez-Lima
RATIONALE: DNA quadruplex structures have emerged as novel drug targets, owing to their role in preventing abnormal gene transcription and maintaining telomere stability. Trapped Ion Mobility Spectrometry - Mass Spectrometry (TIMS - MS), combined with theoretical modeling, is a powerful tool for studying the kinetic intermediates of DNA complexes formed in solution and interrogated in the gas-phase after desolvation. METHODS: A TAGGGT ssDNA sequence was purchased and studied in 10 mM ammonium acetate using nESI-TIMS-MS in positive and negative ion mode...
December 3, 2018: Rapid Communications in Mass Spectrometry: RCM
Juan Patricio Anchordoquy, Juan Mateo Anchordoquy, Noelia Nikoloff, Rocío Gambaro, Gisel Padula, Cecilia Furnus, Analía Seoane
The in vitro effect of enrofloxacin (EFZ) was tested on two experimental somatic bovine cells in vitro: peripheral lymphocytes (PLs) and cumulus cells (CCs). The cytotoxicity and genotoxicity of this veterinary antibiotic were assessed using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assays, single-cell gel electrophoresis (SCGE) assay, and cytokinesis-block micronucleus cytome (CBMN cyt) assay. Cells were treated during 24 h, and three concentrations were tested (50 μg/mL, 100 μg/mL, 150 μg/mL)...
November 30, 2018: Environmental Science and Pollution Research International
Bingshao Liang, Jialiang Mai, Yunfeng Liu, Yanmei Huang, Huamin Zhong, Yongqiang Xie, Qiulian Deng, Lianfen Huang, Shuwen Yao, Yanming He, Yan Long, Yiyu Yang, Sitang Gong, Hongling Yang, Zhenwen Zhou
The prevalent Staphylococcus aureus clones and antibiotic susceptibility profiles are known to change dynamically and geographically; however, recent S. aureus strains causing infections in women and children in China have not been characterized. In this study, we analyzed the molecular epidemiology and antimicrobial resistance of S. aureus isolated from patients in four centers for women and children in Guangzhou, China. In total, 131 S. aureus isolates (100 from children and 31 from women) were analyzed by spa typing, multi-locus sequence typing, virulence gene and antimicrobial resistance profiling, staphylococcal chromosomal cassette mec typing, and mutation analyses of rpoB ...
2018: Frontiers in Microbiology
Sun-Hwa Ha, Jae Kwang Kim, Ye Sol Jeong, Min-Kyoung You, Sun-Hyung Lim, Ju-Kon Kim
Carotenoid pigments are valuable components of the human diet. A notable example is β-carotene, or provitamin A, which is converted into the derivatives astaxanthin and capsanthin, via the common intermediate zeaxanthin. To generate rice varieties producing diverse carotenoids beyond β-carotene, we specifically used a Capsicum β-carotene hydroxylase gene, B (CaBch) and a codon optimized version of the same gene, stB (stBch) to increase zeaxanthin synthesis. We also used a recombinant BAK gene (CaBch-2A-HpBkt), consisting of the CaBch sequence and a Haematococcus β-carotene ketolase gene (HpBkt) linked by a bicistronic 2A sequence, as well as a codon optimized recombinant stBAK gene (stBch-2A-stBkt) to create astaxanthin synthesis...
November 29, 2018: Metabolic Engineering
Vladimir Lopez, Elisabeth van der Heijden, Margarita Villar, Anita Michel, Pilar Alberdi, Christian Gortázar, Victor Rutten, José de la Fuente
There is an imperative need for effective control of bovine tuberculosis (BTB) on a global scale and vaccination of cattle may prove to be pivotal in achieving this. The oral and parenteral use of a heat-inactivated Mycobacterium bovis (M. bovis) vaccine has previously been found to confer partial protection against BTB in several species. A role for complement factor C3 has been suggested in wild boar, but the exact mechanism by which this vaccine provides protection remains unclear. In the present study, a quantitative proteomics approach was used to analyze the white blood cell proteome of vaccinated cattle in comparison to unvaccinated controls, prior (T0) and in response to vaccination, skin test and challenge (T9 and T12)...
December 2018: Veterinary Immunology and Immunopathology
Isabell Tunn, Alberto S de Léon, Kerstin G Blank, Matthew J Harrington
Coiled coils (CCs) have emerged as versatile building blocks for the synthesis of nanostructures, drug delivery systems and biomimetic hydrogels. Bioengineering metal coordination sites into the terminal ends of a synthetic coiled coil (CC), we generate a nanoscale biological building block with tunable stability. The reversible coordination of Ni2+ thermodynamically stabilizes the CC, as shown with circular dichroism spectroscopy. Using atomic force microscopy-based single-molecule force spectroscopy, it is further shown that Ni2+-binding reinforces the CC mechanically, increasing the barrier height for dissociation...
November 30, 2018: Nanoscale
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