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Hongzhi Liu, Hao Su, Xin Wang, Wei Hao
The purpose of this study was to investigate the underlying molecular mechanisms of fracture healing mediated by bone marrow mesenchymal stem cells. Differentially expressed microRNAs in acutely injured subjects and healthy volunteers were screened by microarray analysis. The dual luciferase reporter system was used to verify whether insulin-like growth factor 1 (IGF1) was the direct target gene regulated by miR-148a. The expression level of miR-148a and IGF1 after osteogenic differentiation was detected by quantitative real-time polymerase chain reaction...
October 18, 2018: Journal of Cellular Biochemistry
Dawoon Baek, Kyoung-Mi Lee, Ki Won Park, Jae Wan Suh, Seong Mi Choi, Kwang Hwan Park, Jin Woo Lee, Sung-Hwan Kim
Traumatic and degenerative lesions of articular cartilage usually progress to osteoarthritis (OA), a leading cause of disability in humans. MicroRNAs (miRNAs) can regulate the differentiation of human bone marrow-derived mesenchymal stem cells (hBMSCs) and play important roles in the expression of genes related to OA. However, their functional roles in OA remain poorly understood. Here, we have examined miR-449a, which targets sirtuin 1 (SIRT1) and lymphoid enhancer-binding factor-1 (LEF-1), and observed its effects on damaged cartilage...
September 27, 2018: Molecular Therapy. Nucleic Acids
Zun-Wen Lin, Wei Zhang, Si-Ding Jiang, Wen-Bo Wei, Xiao-Feng Li
Osteosarcoma (OS) is the most common malignant tumor of bone with a high potential for metastasis. This study intends to explore whether microRNA-940 (miR-940) affects the development of OS cells and the underlying mechanism. OS and adjacent normal tissues were collected from OS patients; the OS cell line with the highest expression of miR-940 was selected, which was then subjected to transfection of miR-940 mimic, miR-940 inhibitor, siRNA-secreted frizzled-related protein 1 (SFRP1) or LiCl (agonists of Wnt/β-catenin pathway) to identify regulation of miR-940 to OS cells through SFRP1...
October 15, 2018: Journal of Cellular Biochemistry
Xiaogang Guo, Tao Han, Pingping Hu, Xiaojun Guo, Changming Zhu, Youbao Wang, Shaoyan Chang
BACKGROUND: Prostate cancer (PCa) is a common malignant human tumor and one of the main causes of cancer-related deaths in men. At present, prostate-specific antigen levels are widely used to diagnose PCa in the clinic, but they are not sufficient for an accurate early diagnosis or prognosis. METHODS: To identify potential molecular markers for PCa, we used real-time PCR to measure the expression levels of various microRNAs, including miR-1825, miR-484, miR-205, miR-141, and let-7b, in the serum of 72 PCa patients and 34 healthy controls...
October 15, 2018: International Urology and Nephrology
Aldo H De-La-Cruz-Montoya, Eric G Ramírez-Salazar, Mayeli M Martínez-Aguilar, Pablo M González-de-la-Rosa, Manuel Quiterio, Cei Abreu-Goodger, Jorge Salmerón, Rafael Velázquez-Cruz
Osteoporosis is the most frequent disorder of bone metabolism, owing to an alteration between osteoclast and osteoblast activity, which results from the interaction of genetic, environmental, and epigenetic factors. microRNAs, small non-coding RNAs of around 22 nucleotides with important regulatory roles in gene regulation, that target mRNAs for post-transcriptional destabilization have been suggested as biomarkers in several disorders. In this work, we used small RNA sequencing to identify microRNAs from peripheral blood monocytes that were differentially expressed between non-osteoporotic and osteoporotic Mexican postmenopausal women, to elucidate the potential role of microRNAs as non-invasive marker candidates in osteoporosis...
October 15, 2018: Experimental Biology and Medicine
Ruben Aquino-Martinez, Joshua N Farr, Megan M Weivoda, Brittany A Negley, Jennifer L Onken, Brianne S Thicke, McKenzie M Fulcer, Daniel G Fraser, Andre J van Wijnen, Sundeep Khosla, David G Monroe
Developing novel approaches to treat skeletal disorders requires an understanding of how critical molecular factors regulate osteoblast differentiation and bone remodeling. We have reported that (1) retinoic acid receptor-related orphan receptor beta (Rorβ) is upregulated in bone samples isolated from aged mice and humans in vivo; (2) Rorβ expression is inhibited during osteoblastic differentiation in vitro; and (3) genetic deletion of Rorβ in mice results in preservation of bone mass during aging. These data establish that Rorβ inhibits osteogenesis and that strict control of Rorβ expression is essential for bone homeostasis...
October 15, 2018: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
Na Li, Wen-Bin Wang, Han Bao, Qian Shi, Zong-Lai Jiang, Ying-Xin Qi, Yue Han
Endothelial progenitor cells (EPCs) are vital to the recovery of endothelial function and maintenance of vascular homeostasis. EPCs mobilize to sites of vessel injury and differentiate into mature endothelial cells (ECs). Locally mobilized EPCs are exposed to cyclic stretch caused by blood flow, which is important for EPC differentiation. MicroRNAs (miRNAs) have emerged as key regulators of several cellular processes. However, the role of miRNAs in cyclic stretch-induced EPC differentiation remains unclear...
October 15, 2018: Journal of Cellular Biochemistry
Edwin E Reza-Zaldivar, Mercedes A Hernández-Sapiéns, Benito Minjarez, Yanet K Gutiérrez-Mercado, Ana L Márquez-Aguirre, Alejandro A Canales-Aguirre
Alzheimer's disease (AD) is the most common type of dementia affecting regions of the central nervous system that exhibit synaptic plasticity and are involved in higher brain functions such as learning and memory. AD is characterized by progressive cognitive dysfunction, memory loss and behavioral disturbances of synaptic plasticity and energy metabolism. Cell therapy has emerged as an alternative treatment of AD. The use of adult stem cells, such as neural stem cells and Mesenchymal Stem Cells (MSCs) from bone marrow and adipose tissue, have the potential to decrease cognitive deficits, possibly by reducing neuronal loss through blocking apoptosis, increasing neurogenesis, synaptogenesis and angiogenesis...
2018: Frontiers in Cellular Neuroscience
Jun Liu, Zeng-Yu Zhang, Hong Yu, Ai-Ping Yang, Ping-Fang Hu, Zhuo Liu, Min Wang
Previous studies have revealed that long noncoding RNA (lncRNA) and microRNA play a crucial role in autism, which is a childhood neurodevelopmental disorder with complicated genetic origins. Hence, the study concerns whether lncRNA C21orf121/bone morphogenetic proteins 2 (BMP2)/miR-140-5p gene network affects directed differentiation of stem cells from human exfoliated deciduous teeth (SHED) to neuronal cells in rats with autism. Autism models were successfully established. The neuron cells that differentiated from SHED cell were identified...
October 14, 2018: Journal of Cellular Biochemistry
Ji-Gang He, Qiao-Li Xie, Bei-Bei Li, Liang Zhou, Dan Yan
BACKGROUND: The immunosuppressive activity of mesenchymal stem cells (MSCs) has been exploited to induce tolerance after organ transplantation. The indoleamine 2,3-dioxygenase (IDO) may have beneficial effects in the immunoregulatory properties of MSCs. It was recently revealed that exosomes derived from MSCs play important roles in mediating the biological functions of MSCs. This study aimed to explore the roles of exosomes derived from MSCs in the induction of immune tolerance. METHODS: Dendritic cells (DCs) and T-cells were cultured with exosomes derived from rat bone marrow MSCs (BMSCs) overexpressing IDO1 or controls...
October 12, 2018: Cell Transplantation
Tstutomu Kawaguchi, Li Yan, Qianya Qi, Xuan Peng, Stephen B Edge, Jessica Young, Song Yao, Song Liu, Eigo Otsuji, Kazuaki Takabe
BACKGROUND: The use of biomarkers that allow early therapeutic intervention or intensive follow-up evaluation is expected to be a powerful means for reducing breast cancer mortality. MicroRNAs (miRNAs) are known to play major roles in cancer biology including metastasis. This study aimed to develop a novel miRNA risk score to predict patient survival and metastasis in breast cancer. METHODS: An integrated unbiased approach was applied to derive a composite risk score for prognosis based on miRNA expression in primary breast tumors in 1051 breast cancer patients from The Cancer Genome Atlas (TCGA)...
October 11, 2018: Annals of Surgical Oncology
Zong-Yuan Jiang, Jun-Jian Jiang, Yu-Shui Ma, Hong-Ye Li, Wei Shi, Pei-Liang Fu, Cong-Feng Xu, Jiu-Zhou Lu, Da Fu, Jian-Guang Xu
Giant-cell tumor (GCT) of the bone is an invasiveness and high recurrent bone tumor that is considered borderline or potentially malignant. To explore the molecular mechanism leading to bone destruction and identify novel targets for treatment, we conducted silencing of miR-223 and miR-19a in stromal giant cells and identified TWIST and Runx2 as their target genes. We investigated the impact of these microRNAs and their target genes on stromal giant cells that promote the differentiation of monocyte/macrophages into osteoclast cells and recruitment to the bone microenvironment, which in turn enhances the bone destruction capacity of GCT...
October 8, 2018: Biochemical and Biophysical Research Communications
Wei Wang, Zijun Guo, Hong Yu, Ling Fan
Osteosarcoma (OS) is considered the most common type of primary malignant bone tumor, which has a high rate of mortality in children and adolescents. However, the current treatment methods for OS are ineffective. Therefore, there is an urgent requirement to identify the critical targets. This study aimed to identify the roles and significance of microRNA-216b (miR-216b) in OS. To explore the cellular and molecular functions of miR-216b and Forkhead Box M1 (FoxM1) in OS, the expression of miR-216b and FoxM1 at the transcriptional level was measured using quantitative real-time PCR (qRT-PCR)...
October 9, 2018: Journal of Cellular Biochemistry
Kazuki Inoue, Zhonghao Deng, Yufan Chen, Eugenia Giannopoulou, Ren Xu, Shiaoching Gong, Matthew B Greenblatt, Lingegowda S Mangala, Gabriel Lopez-Berestein, David G Kirsch, Anil K Sood, Liang Zhao, Baohong Zhao
Targeting microRNAs recently shows significant therapeutic promise; however, such progress is underdeveloped in treatment of skeletal diseases with osteolysis, such as osteoporosis and rheumatoid arthritis (RA). Here, we identified miR-182 as a key osteoclastogenic regulator in bone homeostasis and diseases. Myeloid-specific deletion of miR-182 protects mice against excessive osteoclastogenesis and bone resorption in disease models of ovariectomy-induced osteoporosis and inflammatory arthritis. Pharmacological treatment of these diseases with miR-182 inhibitors completely suppresses pathologic bone erosion...
October 5, 2018: Nature Communications
Christopher T Esapa, Sian E Piret, M Andrew Nesbit, Gethin P Thomas, Leslie A Coulton, Orla M Gallagher, Michelle M Simon, Saumya Kumar, Ann-Marie Mallon, Ilaria Bellantuono, Matthew A Brown, Peter I Croucher, Paul K Potter, Steve Dm Brown, Roger D Cox, Rajesh V Thakker
Kyphosis and scoliosis are common spinal disorders that occur as part of complex syndromes or as nonsyndromic, idiopathic diseases. Familial and twin studies implicate genetic involvement, although the causative genes for idiopathic kyphoscoliosis remain to be identified. To facilitate these studies, we investigated progeny of mice treated with the chemical mutagen N -ethyl- N -nitrosourea (ENU) and assessed them for morphological and radiographic abnormalities. This identified a mouse with kyphoscoliosis due to fused lumbar vertebrae, which was inherited as an autosomal dominant trait; the phenotype was designated as hereditary vertebral fusion (HVF) and the locus as Hvf ...
May 2018: JBMR Plus
Chenyu Wang, Ming Ren, Xin Zhao, Ao Wang, Jincheng Wang
Osteosarcoma (OS) is a primary malignant bone tumor in early adolescence with high metastasis and death rates. Although the combination of polychemotherapy and surgical excision increased the survival rates up to 60%, the prognosis remains poor for most patients with metastatic or recurrent osteosarcoma. However, the exact pathogenic mechanism and pivotal elements regulating tumor invasion and metastasis are largely unknown. Circular RNAs (circRNAs) are novel endogenous non-coding RNA (ncRNA) molecules that generate the cyclic structure from back splicing...
October 4, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Hao Sun, Shu Hu, Ziji Zhang, Jiayong Lun, Weiming Liao, Zhiqi Zhang
The aim of the current study was to compare the expression of microRNAs (miRNAs) in exosomes derived from human bone mesenchymal stem cells (hBMSCs) with and without chondrogenic induction. Exosomes derived from hBMSCs were isolated and identified. Microarray analysis was performed to compare miRNA expression between exosomes derived from hBMSCs with and without chondrogenic induction, and quantitative real-time polymerase chain reaction (qRT-PCR) was used to verify the differentially expressed miRNAs. hBMSCs were transfected with miRNA mimic to extract miRNA-overexpressed exosomes...
September 11, 2018: Journal of Cellular Biochemistry
Maryam Izadpanahi, Ehsan Seyedjafari, Ehsan Arefian, Ahmad Hamta, Simzar Hosseinzadeh, Mousa Kehtari, Masoud Soleimani
Application of stem cells in combination with nanofibrous substrates is an interesting biomimetic approach for enhanced regeneration of damaged tissues such as bone and cartilage. The investigation of the complex interplay between nanotopographical cues of niche and noncoding RNAs in stem cells fate is an effective tool to find a new strategy for enhancing the induction of osteogenesis. In this study, we investigated the effects of aligned and random orientations of nanofibers as a natural ECM-mimicking environment on the network of noncoding RNA in mesenchymal stem cells...
December 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Lei Guo, Kaizhe Chen, Jun Yuan, Ping Huang, Xing Xu, Changwei Li, Niandong Qian, Jin Qi, Zhiliang Shao, Lianfu Deng, Chuan He, Jiping Xu
Inhibition of osteoclasts formation and bone resorption by estrogen is very important in the etiology of postmenopausal osteoporosis. The mechanisms of this process are still not fully understood. Recent studies implicated an important role of microRNAs in estrogen-mediated responses in various cellular processes, including cell differentiation and proliferation. Thus, we hypothesized that these regulatory molecules might be implicated in the process of estrogen-decreased osteoclasts formation and bone resorption...
September 10, 2018: Journal of Cellular Physiology
Yun-Jie Zhang, Yu-Shui Ma, Qing Xia, Fei Yu, Zhong-Wei Lv, Cheng-You Jia, Xiao-Xin Jiang, Liang Zhang, Yun-Chao Shao, Wen-Ting Xie, Gai-Xia Lu, Xia-Qing Yv, Peng Zhong, Da Fu, Xiao-Feng Wang
The incidence of well‑differentiated thyroid cancer (WDTC) is rapidly increasing. Poor survival follows distant metastasis (DM) and recurrence. In the present study, we aimed to analyze the expression alterations in different stages of WDTC and the regulatory mechanism of DM and the recurrence of DM. A male patient diagnosed with follicular thyroid cancer and distant metastasis in the eleventh thoracic vertebrae received total thyroidectomy and the removal of a metastatic lesion. A local relapse was found in the vertebrae after four‑time iodine‑131 treatment...
September 26, 2018: Oncology Reports
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