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Rajab Mardani, Mohammad Hassan Jafari Najaf Abadi, Mahsa Motieian, Sima Taghizadeh-Boroujeni, Amir Bayat, Alireza Farsinezhad, Seyed Mohammad Gheibi Hayat, Mahtab Motieian, Hossein Pourghadamyari
Leukemia is known as a progressive malignant disease, which destroys the blood-forming organs and results in adverse effects on the proliferation and development of leukocytes and their precursors in the blood and bone marrow. There are four main classes of leukemia including acute leukemia, chronic leukemia, myelogenous leukemia, and lymphocytic leukemia. Given that a variety of internal and external factors could be associated with the initiation and progression of different types of leukemia. One of the important factors is epigenetic regulators such as microRNAs (miRNAs) and long noncoding RNAs (ncRNA)...
December 4, 2018: Journal of Cellular Physiology
Steven Georges, Lidia Rodriguez Calleja, Camille Jacques, Melanie Lavaud, Brice Moukengue, Fernando Lecanda, Thibaut Quillard, Marta Tellez Gabriel, Pierre-François Cartron, Marc Baud'huin, François Lamoureux, Dominique Heymann, Benjamin Ory
The metastatic dissemination is a complex multistep process by which tumor cells from a primary site enter into the systemic circulation to finally spread at distant sites. Even if this mechanism is rare at the tumor level, it remains the major cause of Osteosarcoma-patients' relapse and mortality. MicroRNAs (miRNAs) have recently been described as novel epigenetics' genes' expression regulators actively implicated in cancer progression and dissemination. The understanding of their implication in the metastatic spreading could help clinicians to improve the outcome of osteosarcoma...
November 6, 2018: Oncotarget
C Simon Durai Raj, Krishnaraj Thirugnanasambantham, Mohamed Ibrahim Hairul Islam, S Mathan Kumar, Sandhya Sundaram, Ganapathy Ashok, Venugopal Senthilkumar, Senguttuvan Muralidaran, S Saravanan
BACKGROUND: Rheumatoid arthritis (RA) is a chronic inflammatory and autoimmune disease leading to bones and joints destruction. It is one of the major causes of lifetime disability and mortality among humans in the developing and developed countries. It was evident that epigenetic dysregulation is related to the pathogenesis of RA. MicroRNAs (miRNAs) are small non-coding RNAs that are epigenetic regulators for diverse biological processes and also provided novel molecular insights in the formation of arthritis...
December 3, 2018: MicroRNA
Fernanda T Borges, Marcia Bastos Convento, Nestor Schor
Bone marrow mesenchymal stromal cell (MSC) is a potential alternative in regenerative medicine and has great potential in many pathologic conditions including kidney disease. Although most of the studies demonstrate MSC efficiency, the regenerative potential may not be efficient in all diseases and patients. Stem cell feasibility is modified by donor characteristics as gender, age, diet, and health status, producing both positive and negative results. The conditioning of MSC can potentiate its effects and modify its culture medium (CM)...
2018: Stem Cells and Cloning: Advances and Applications
Ling Xu, Yu Fu, Weiwen Zhu, Rongyao Xu, Juan Zhang, Ping Zhang, Jie Cheng, Hongbing Jiang
BACKGROUND: Cleidocranial dysplasia (CCD) in humans is an autosomal-dominant skeletal dysplasia caused by heterozygous mutations of the runt-related transcription factor 2 (RUNX2) and significantly increases the risk of osteoporosis. Increasing evidence demonstrates that the dysfunction of bone marrow stromal cells from CCD patients (BMSCs-CCD) contributes to the bone deficiency, but the characteristics of BMSCs-CCD and the mechanisms that underlie their properties remain undefined. METHODS: The clinical manifestations of three CCD patients were collected and the mutations of RUNX2 were analyzed...
December 3, 2018: Journal of Cellular Biochemistry
Hui Ren, Xiang Yu, Gengyang Shen, Zhida Zhang, Qi Shang, Wenhua Zhao, Jinjing Huang, Peiyuan Yu, Meiqi Zhan, Yongqiang Lu, Ziyang Liang, Jingjing Tang, De Liang, Zhensong Yao, Zhidong Yang, Xiaobing Jiang
High-throughput sequencing (HTS) was recently applied to detect microRNA (miRNA) regulation in age-related osteoporosis. However, miRNA regulation has not been reported in glucocorticoid-induced osteoporosis (GIOP) patients and the mechanism of GIOP remains elusive. To comprehensively analyze the role of miRNA regulation in GIOP based on human vertebrae and to explore the molecular mechanism, a high-throughput sequencing strategy was employed to identify miRNAs involved in GIOP. Twenty-six patients undergoing spinal surgery were included in this study...
November 29, 2018: Bone
Silin Gan, Ping Ma, Jie Ma, Weimin Wang, Haohao Han, Li Chen, Xue Li, Feifei Wu, Hui Sun
Leukemia is the most frequent malignancy in children with acute myeloid leukemia (AML) as the second commonest type. Long non-coding RNA zinc finger antisense 1 (ZFAS1) has been widely reported as an oncogenic factor in multiple malignancies including AML. However, the roles and molecular mechanisms of ZFAS1 in the tumorigenesis of AML are poor defined till now. In the present study, RT-qPCR assay showed that ZFAS1 was highly expressed in bone marrow of acute leukemia patients and AML cell lines. Loss-of-function analyses revealed that ZFAS1 knockdown inhibited proliferation and promoted apoptosis in AML cells and curbed AML xenograft growth in vivo...
November 28, 2018: European Journal of Pharmacology
Mi Zhou, Bo Jiang, Mao Xiong, Xin Zhu
Aims: Rheumatoid arthritis (RA) is characterized by cartilage and bone damage leading to disability. Here, the association between microRNA (miRNA) polymorphisms and susceptibility to RA was evaluated by performing an updated meta-analysis and systematic review. Main methods: An electronic search of databases including PubMed and Embase was performed from inception to December 8, 2017 to retrieve studies investigating the association between miRNA polymorphisms and RA risk. Two reviewers independently screened literature according to the inclusion and exclusion criteria and extracted data...
2018: Frontiers in Physiology
Kanokwan Seenprachawong, Tulyapruek Tawornsawutruk, Chanin Nantasenamat, Pornlada Nuchnoi, Suradej Hongeng, Aungkura Supokawej
Mesenchymal stem cell (MSC) is a type of stem cell that is capable of differentiating into osteoblasts and adipocytes. The pathological perturbation of MSC fate determination is well demonstrated by the replacement of bone tissues with fat in those with osteoporosis and osteopenia. Cell fate determination can be regulated by epigenetic and post-transcriptional mechanisms. MicroRNAs (miRNAs) are small endogenous non-coding RNA molecules that mediates the post-transcriptional regulation of genes expression. We hypothesized that miRNA specified to PPAR γ, a major transcription factor of adipogenesis, is responsible for the differentiation of MSCs into osteoblasts...
2018: Frontiers in Genetics
Giuseppina E Grieco, Dorica Cataldo, Elena Ceccarelli, Laura Nigi, Giovanna Catalano, Noemi Brusco, Francesca Mancarella, Giuliana Ventriglia, Cecilia Fondelli, Elisa Guarino, Isabella Crisci, Guido Sebastiani, Francesco Dotta
Type 1 diabetes (T1D) is characterized by bone loss and altered bone remodeling, resulting into reduction of bone mineral density (BMD) and increased risk of fractures. Identification of specific biomarkers and/or causative factors of diabetic bone fragility is of fundamental importance for an early detection of such alterations and to envisage appropriate therapeutic interventions. MicroRNAs (miRNAs) are small non-coding RNAs which negatively regulate genes expression. Of note, miRNAs can be secreted in biological fluids through their association with different cellular components and, in such context, they may represent both candidate biomarkers and/or mediators of bone metabolism alterations...
November 27, 2018: Non-Coding RNA
Laura De-Ugarte, Susana Balcells, Xavier Nogues, Daniel Grinberg, Adolfo Diez-Perez, Natalia Garcia-Giralt
MicroRNAs (miRNAs) are important regulators of many cellular processes, including the differentiation and activity of osteoblasts, and therefore, of bone turnover. MiR-320a is overexpressed in osteoporotic bone tissue but its role in osteoblast function is unknown. In the present study, functional assays were performed with the aim to elucidate the mechanism of miR-320a action in osteoblastic cells. MiR-320a was either overexpressed or inhibited in human primary osteoblasts (hOB) and gene expression changes were evaluated through microarray analysis...
2018: PloS One
Feng Jia, Zhen Zhang, Xu Zhang
Osteosarcoma (OS) is one of the most aggressive bone tumors. MicroRNAs (miRNAs) have been found to implicate in the pathogenesis of different types of cancers, including OS. This study aimed to explore the roles of miR-338-3p in OS and investigate the underlying mechanism. Human OS cell lines (MG-63 and U2OS) and osteoblast (hFOB) cell line were used in the study. The expression levels of miR-338-3p, runt-related transcription factor 2 (RUNX2) and cyclin-dependent kinase 4 (CDK4) were altered by transient transfection and determined by quantitative real-time polymerase chain reaction/Western blot analysis...
November 28, 2018: Journal of Cellular Biochemistry
Francesca Lovat, Matteo Fassan, Diana Sacchi, Parvathi Ranganathan, Alexey Palamarchuk, Marius Bill, Malith Karunasiri, Pierluigi Gasparini, Giovanni Nigita, Rosario Distefano, Dario Veneziano, Adrienne M Dorrance, Ramiro Garzon, Carlo M Croce
MicroRNAs (miRNAs) have been extensively reported to be associated with hematological malignancies. The loss of miR-15a/16-1 at chromosome 13q14 is a hallmark of most of human chronic lymphocytic leukemia (CLL). Deletion of murine miR-15a/16-1 and miR-15b/16-2 has been demonstrated to promote B cell malignancies. Here, we evaluate the biological role of miR-15/16 clusters, crossbreeding miR-15a/16-1 and miR-15b/16-2 knockout mice. Unexpectedly, the complete deletion of both clusters promoted myeloproliferative disorders in the majority of the mice by the age of 5 months with a penetrance of 70%...
November 26, 2018: Proceedings of the National Academy of Sciences of the United States of America
Yves-Marie Pers, Marie Maumus, Claire Bony, Christian Jorgensen, Danièle Noël
Mesenchymal stem/stromal cells (MSCs) are progenitor cells identified in many adult tissues, in particular in bone marrow or adipose tissue and, in placental tissues. MSCs exert pleiotropic functions that render them attractive for many clinical applications, both in degenerative and inflammatory diseases. Their main mode of action is through the secretion of trophic factors that can be released in the extracellular milieu or packaged within extracellular vesicles. These factors can be proteins, lipids, mRNAs or miRNAs that possess diverse effects, notably pro-angiogenic, anti-fibrotic, anti-apoptotic or anti-inflammatory...
December 2018: Biochimie
Yanhong Li, Jie Liu, Jiaojiao Zhang, Wenjin Zhang, Zhengli Wu
BACKGROUND: Mast cells play a central role in innate and adaptive immunity by releasing pre-formed and de novo synthesized mediators, which include microRNAs. Although miRNAs have been confirmed to function in cell proliferation, differentiation, apoptosis, and the immune response, their functions are still limited in mast cells degranulation. METHODS: Here, we survey miRNA expression profiles in activated mouse bone marrow-derived mast cells (BMMCs) with a miRNA microarray and compare the profiles to those from resting BMMCs...
November 9, 2018: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
L-M Chao, W Sun, H Chen, B-Y Liu, P-F Li, D-W Zhao
OBJECTIVE: To elucidate the role of microRNA-31 (miR-31) in osteosarcoma and the molecular mechanism of miR-31 in the proliferation, migration, and invasion of osteosarcoma. PATIENTS AND METHODS: Real Time-quantitative Polymerase Chain Reaction (RT-qPCR) was used to examine the expression of microRNA-31 in human osteosarcoma tissues. Pearson's chi-squared test was used to analyze the correlation between microRNA-31 and clinicopathological features. Proliferation, migration, invasion, and PI3K3C2A protein in treated osteosarcoma cells were detected by Cell Counting Kit-8 (CCK-8) assay, transwell assay without Matrigel, transwell assay with Matrigel, and Western blot analysis, respectively...
November 2018: European Review for Medical and Pharmacological Sciences
M-H Sun, W-J Wang, Q Li, T Yuan, W-J Weng
OBJECTIVE: Suitable seed cells and selection of bioactive scaffold materials are the main research contents of bone tissue engineering. It was showed that autologous oxygen release nano bionic scaffold could promote the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). The role of microRNA-106a (miR-106a) in regulating BMSCs differentiation has not been reported. We intend to investigate the role of autologous oxygen release nano bionic scaffold composite miR-106a in inducing BMSCs constructing tissue engineering bone...
November 2018: European Review for Medical and Pharmacological Sciences
Ziye Li, Jianmin Luo
Purpose: Chronic myeloid leukemia (CML) accounts for ~10% of leukemia cases, and its progression involves epigenetic gene regulation. This study investigated epigenetic regulation of HOTAIR and its target microRNA, miR-143, in advanced CML. Patients and methods: We first isolated bone marrow mononuclear cells from 70 patients with different phases of CML and from healthy donors as normal control; we also cultured K562 and KCL22 cells, treated with demethylation drug; MTT assay, flow cytometry, quantitative real-time polymerase chain reaction (qPCR), methylation-specific polymerase chain reaction (MSP), Western blot, luciferase assay, RNA pull-down assay and RNA-binding protein immunoprecipitation (RIP) assay were performed...
2018: Cancer Management and Research
Xiaowu Chen, Jianpeng Chen, Yawei Shen, Yanhui Bi, Wenjie Hou, Guiping Pan, Xugan Wu
The swimming crab (Portunus trituberculatus, Portunus) can tolerate low salinity, but the mechanism of its varied salinity adaptation at the molecular level remains unclear. In this study, we prepared four mRNA and microRNA (miRNA) libraries using the gills collected from four salinity groups and performed RNA-sequencing (RNA-Seq) to identify the genes related to the low salinity. We set 25 ppt as the control group. A total of 659 genes were differentially expressed in at least one of the six comparison groups (25 ppt vs...
November 7, 2018: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
Hu-Cheng Liu, Jin Zeng, Bin Zhang, Xu-Qiang Liu, Min Dai
Osteosarcoma (OS) is one of the most common primary bone malignancies, with the survival rate of patients with OS remaining low. Therefore, we conducted this study to identify the potential role combination of both MSH6 gene silencing and cisplatin (DDP) plays in OS cell proliferation and apoptosis. Microarray-based gene expression profiling was used to identify the differentially expressed genes (DEGs) in patients with OS, as well as microRNAs (miRNAs) that regulate the candidate gene. OS tissues from 67 patients with OS along with normal tissues from 24 amputee patients were collected for detection of the positive expression of mutS homolog 6 (MSH6) protein, mRNA, and protein expressions of c-myc, cyclin D1, l-2, B-cell lymphoma 2 (Bcl-2), Stathmin, proliferating cell nuclear antigen (PCNA), and Bcl-2-associated X (Bax)...
November 19, 2018: Journal of Cellular Physiology
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