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DERMAL ADIPOSE

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https://www.readbyqxmd.com/read/30092820/adipose-tissue-derived-extracellular-fraction-characterization-biological-and-clinical-considerations-in-regenerative-medicine
#1
Barbara Bellei, Emilia Migliano, Marinella Tedesco, Silvia Caputo, Federica Papaccio, Gianluca Lopez, Mauro Picardo
BACKGROUND: Adipose tissue-derived stem cells are considered to be a promising source in the field of cell therapy and regenerative medicine. In addition to direct cell replacement using adipose tissue or purified stem cells, intercellular molecule exchange by the adipose tissue complex, a vast array of bioactive secretory factors, demonstrated beneficial effects by reducing tissue damage and stimulation of endogenous repair. However, for therapeutic purposes, the use of secretome derivatives, such as full conditioned media or purified exosomes generated in vitro, may present considerable disadvantages for cell manufacturing, storage, product safety, and their potential as a ready-to-go therapeutic product...
August 9, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/30087356/an-orally-active-adiponectin-receptor-agonist-mitigates-cutaneous-fibrosis-inflammation-and-microvascular-pathology-in-a-murine-model-of-systemic-sclerosis
#2
Takashi Yamashita, Katja Lakota, Takashi Taniguchi, Ayumi Yoshizaki, Shinichi Sato, Wen Hong, Xingchun Zhou, Snezn Sodin-Semrl, Feng Fang, Yoshihide Asano, John Varga
The hallmarks of systemic sclerosis (SSc) are autoimmunity, microangiopathy and fibrosis. Skin fibrosis is accompanied by attrition of the dermal white adipose tissue layer, and alterations in the levels and function of adiponectin. Since these findings potentially implicate adiponectin in the pathogenesis of SSc, we employed a novel pharmacological approach to augment adiponectin signaling using AdipoRon, an orally active adiponectin receptor agonist. Chronic treatment with AdipoRon significantly ameliorated bleomycin-induced dermal fibrosis in mice...
August 7, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30030486/prevascularization-of-dermal-substitutes-with-adipose-tissue-derived-microvascular-fragments-enhances-early-skin-grafting
#3
Florian S Frueh, Thomas Später, Christina Körbel, Claudia Scheuer, Anna C Simson, Nicole Lindenblatt, Pietro Giovanoli, Michael D Menger, Matthias W Laschke
Split-thickness skin grafts (STSG) are still the gold standard for the treatment of most skin defects. Hence, there is an ongoing need to improve this procedure. For this purpose, we herein analyzed dermal matrices seeded with adipose tissue-derived microvascular fragments (ad-MVF) in a bradythrophic wound model. In additional experiments, the matrices were covered with autologous STSG 10 days after implantation. Green fluorescence protein (GFP)+ ad-MVF were isolated from C57BL/6-Tg(CAG-EGFP)1Osb/J mice and seeded onto collagen-glycosaminoglycan matrices...
July 20, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30022246/fgf-2-combined-with-bilayer-artificial-dermis-composed-of-collagen-matrix-prompts-generation-of-fat-pad-in-subcutis-of-mice
#4
Natsuko Kakudo, Naoki Morimoto, Takeshi Ogawa, Shigeru Taketani, Kenji Kusumoto
Fibroblast growth factor (FGF)-2 induces mitogenesis, angiogenesis and adipogenesis. In this study, the adipogenesis-inducing effects of FGF-2 combined with bilayer artificial dermis in mice were evaluated. FGF-2-impregnated bilayer artificial dermis composed of collagen matrix, PELNAC (Gunze Corp., Osaka, Japan) was implanted subcutaneously into the thoracic region of mice. At 1, 2, 3, and 4 weeks, samples were collected for H&E staining, von Willebrand factor immunostaining, and perilipin immunostaining to examine adipose tissue localization and angiogenesis...
July 18, 2018: Medical Molecular Morphology
https://www.readbyqxmd.com/read/30006585/inflammation-linked-adaptations-in-dermal-microvascular-reactivity-accompany-the-development-of-obesity-and-type-2-diabetes
#5
Marie-Sophie Nguyen-Tu, Pierre Nivoit, Valérie Oréa, Sandrine Lemoine, Cécile Acquaviva, Aurélie Pagnon-Minot, Bérengère Fromy, Jaswinder K Sethi, Dominique Sigaudo-Roussel
BACKGROUND/OBJECTIVES: The increased prevalence of obesity has prompted great strides in our understanding of specific adipose depots and their involvement in cardio-metabolic health. However, the impact of obesity on dermal white adipose tissue (dWAT) and dermal microvascular functionality remains unclear. This study aimed to investigate the temporal changes that occur in dWAT and dermal microvascular functionality during the development of diet-induced obesity and type 2 diabetes in mice...
July 13, 2018: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29987390/bone-marrow-derived-versus-adipose-derived-stem-cells-in-wound-healing-value-and-route-of-administration
#6
Basma Emad Aboulhoda, Shereen Abd El Fattah
The stem cells with their distinct ability of self-renewal and differentiation are considered an innovation in wound healing. However, there is lack of studies comparing the differential effect of the type and administration route of stem cells in the wound healing context. Thus, the current study has been designed to elucidate the effect of two of the most important stem cell types-the bone marrow-derived and adipose-derived stem cells in full thickness wound healing-and to evaluate, in this optimized wound model, the effectiveness of intradermal versus intravenous routes using H&E, Masson's trichrome, and PKH26-stained sections...
July 10, 2018: Cell and Tissue Research
https://www.readbyqxmd.com/read/29981277/improved-vasculogenesis-and-bone-matrix-formation-through-co-culture-of-endothelial-cells-and-stem-cells-in-tissue-specific-methacryloyl-gelatin-based-hydrogels
#7
Annika Wenz, Iva Tjoeng, Isabelle Schneider, Petra J Kluger, Kirsten Borchers
The co-culture of osteogenic and angiogenic cells and the resulting paracrine signaling via soluble factors is supposed to be crucial for successfully engineering vascularized bone tissue equivalents. In this study, a co-culture system combining primary human adipose-derived stem cells (hASCs) and primary human dermal microvascular endothelial cells (HDMECs) within two types of hydrogels based on methacryl-modified gelatin (GM) as threedimensional (3D) scaffolds was examined regarding its support of tissue specific cell functions...
July 7, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29973295/iberian-pig-mesenchymal-stem-stromal-cells-from-dermal-skin-abdominal-and-subcutaneous-adipose-tissues-and-peripheral-blood-in-vitro-characterization-and-migratory-properties-in-inflammation
#8
Alexandra Calle, Clara Barrajón-Masa, Ernesto Gómez-Fidalgo, Mercedes Martín-Lluch, Paloma Cruz-Vigo, Raúl Sánchez-Sánchez, Miguel Ángel Ramírez
BACKGROUND: Recently, the capacity of mesenchymal stem/stromal cells (MSCs) to migrate into damaged tissues has been reported. For MSCs to be a promising tool for tissue engineering and cell and gene therapy, it is essential to know their migration ability according to their tissue of origin. However, little is known about the molecular mechanisms regulating porcine MSC chemotaxis. The aim of this study was to examine the migratory properties in an inflammatory environment of porcine MSC lines from different tissue origins: subcutaneous adipose tissue (SCA-MSCs), abdominal adipose tissue (AA-MSCs), dermal skin tissue (DS-MSCs) and peripheral blood (PB-MSCs)...
July 4, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29951590/skin-aging-as-a-mechanical-phenomenon-the-main-weak-links
#9
Ilja L Kruglikov, Philipp E Scherer
From a mechanical point of view, human skin appears as a layered composite containing the stiff thin cover layer presented by the stratum corneum, below which are the more compliant layers of viable epidermis and dermis and further below the much more compliant adjacent layer of subcutaneous white adipose tissue (sWAT). Upon exposure to a strain, such a multi-layer system demonstrates structural instabilities in its stiffer layers, which in its simplest form is the wrinkling. These instabilities appear hierarchically when the mechanical strain in the skin exceeds some critical values...
June 15, 2018: Nutrition and Healthy Aging
https://www.readbyqxmd.com/read/29931303/the-effects-of-adipose-stem-cell-conditioned-media-on-fibrogenesis-of-dermal-fibroblasts-stimulated-by-transforming-growth-factor-%C3%AE-1
#10
Bo Yuan, James A Broadbent, Jingning Huan, Huizhong Yang
Adipose-derived stem cells (ASCs) have been shown to enhance wound healing by human dermal fibroblasts; however, the interactions between ASCs and fibroblasts during injury remain unclear. Fibroblasts were treated with ASC-conditioned medium (ASC-CM) with and without transforming growth factor-β1 (TGF-β1) stimulation. Fibroblast proliferation, apoptosis, differentiation and expression of extracellular matrix genes and proteins, type I collagen, and type III collagen were measured. Also, wound-healing effect of ASC-CM was verified with in vivo animal study...
January 1, 2018: Journal of Burn Care & Research: Official Publication of the American Burn Association
https://www.readbyqxmd.com/read/29891910/anti-inflammatory-role-of-15-lipoxygenase-contributes-to-the-maintenance-of-skin-integrity-in-mice
#11
Sang-Nam Kim, Seun Akindehin, Hyun-Jung Kwon, Yeon-Ho Son, Abhirup Saha, Young-Suk Jung, Je-Kyung Seong, Kyung-Min Lim, Jong-Hyuk Sung, Krishna Rao Maddipati, Yun-Hee Lee
15-lipoxygenase is involved in the generation of specialized pro-resolving lipid mediators that play essential roles in resolution and inflammatory responses. Here, we investigated anti-inflammatory role of Alox15 in skin homeostasis. We demonstrated that knockout (KO) of Alox15 led to hair loss and disrupted the structural integrity of the dorsal skin. Alox15 KO resulted in loss of hair follicle stem cells and abnormal transition of dermal adipocytes into fibroblasts. Alox15 deficiency increased infiltration of proinflammatory macrophages and upregulated proinflammatory and necroptotic signaling in dermal adipose tissue in the dorsal skin...
June 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29867527/the-reparative-abilities-of-menstrual-stem-cells-modulate-the-wound-matrix-signals-and-improve-cutaneous-regeneration
#12
Jimena Cuenca, Alice Le-Gatt, Valentina Castillo, Jose Belletti, Macarena Díaz, Mónica Kurte G, Paz L Gonzalez, Francisca Alcayaga-Miranda, Christina M A P Schuh, Fernando Ezquer, Marcelo Ezquer, Maroun Khoury
Considerable advances have been made toward understanding the cellular and molecular mechanism of wound healing, however, treatments for chronic wounds remain elusive. Emerging concepts utilizing mesenchymal stem cells (MSCs) from umbilical cord, adipose tissue and bone marrow have shown therapeutical advantages for wound healing. Based on this positive outcome, efforts to determine the optimal sources for MSCs are required in order to improve their migratory, angiogenic, immunomodulatory, and reparative abilities...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29787514/therapeutic-effects-of-human-adipose-derived-products-on-impaired-wound-healing-in-irradiated-tissue
#13
Szu-Hsien Wu, Takako Shirado, Takanobu Mashiko, Jingwei Feng, Rintaro Asahi, Koji Kanayama, Masanori Mori, Daekwan Chi, Ataru Sunaga, Shunji Sarukawa, Kotaro Yoshimura
BACKGROUND: Clinical sequelae of irradiation result in tissue devitalization (e.g., ischemia, fibrosis, and atrophy) where wound healing capacity is impaired. Fat-derived products may work to treat such pathology. METHODS: Nonlethal irradiation at various doses (5, 10, and 15 Gy) and frequencies (one to three times on sequential days) was delivered to dorsal skin of nude mice, and subsequent gross and microscopic changes were evaluated for up to 4 weeks. Cutaneous punch wounds were then created to compare wound healing in irradiated and nonirradiated states...
August 2018: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/29704126/dermal-white-adipose-tissue-undergoes-major-morphological-changes-during-the-spontaneous-and-induced-murine-hair-follicle-cycling-a-reappraisal
#14
April R Foster, Carina Nicu, Marlon R Schneider, Eleanor Hinde, Ralf Paus
In murine skin, dermal white adipose tissue (DWAT) undergoes major changes in thickness in synchrony with the hair cycle (HC); however, the underlying mechanisms remain unclear. We sought to elucidate whether increased DWAT thickness during anagen is mediated by adipocyte hypertrophy or adipogenesis, and whether lipolysis or apoptosis can explain the decreased DWAT thickness during catagen. In addition, we compared HC-associated DWAT changes between spontaneous and depilation-induced hair follicle (HF) cycling to distinguish between spontaneous and HF trauma-induced events...
July 2018: Archives of Dermatological Research
https://www.readbyqxmd.com/read/29660167/microscopy-with-ultraviolet-surface-excitation-muse-a-novel-approach-to-real-time-inexpensive-slide-free-dermatopathology
#15
Amir Qorbani, Farzad Fereidouni, Richard Levenson, Sana Y Lahoubi, Zachary T Harmany, Austin Todd, Maxwell A Fung
Traditional histology relies on processing and physically sectioning either frozen or formalin-fixed paraffin-embedded (FFPE) tissue into thin slices (typically 4-6 μm) prior to staining and viewing on a standard wide-field microscope. Microscopy using ultraviolet (UV) surface excitation (MUSE) represents a novel alternative microscopy method that works with UV excitation using oblique cis-illumination, which can generate high-quality images from the cut surface of fresh or fixed tissue after brief staining, with no requirement for fixation, embedding and histological sectioning of tissue specimens...
July 2018: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/29621642/adipose-derived-stem-cells-improve-full-thickness-skin-grafts-in-a-rat-model
#16
Silvana Bellini Vidor, Paula Barros Terraciano, Fernanda Soldatelli Valente, Verônica Machado Rolim, Cristiana Palma Kuhl, Laura Silveira Ayres, Tuane Nerissa Alves Garcez, Natália Emerim Lemos, Cristiano Ely Kipper, Sabrina Beal Pizzato, David Driemeier, Elizabeth Obino Cirne-Lima, Emerson Antonio Contesini
To investigate the effects of heterologous adipose-derived stem cells (ADSCs) on autologous full-thickness skin grafts, we designed a first-intention healing model using Wistar rats. We harvested and sutured two full-thickness skin grafts in the dorsal recipient beds of 15 rats, randomized into three groups. In the treatment group, 1 × 106 ADSCs resuspended in saline solution (200 μL) were administered subcutaneously to the skin graft. The control group received only saline solution subcutaneously, whereas the negative control group did not receive any treatment...
June 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29614431/3d-cell-printing-of-in-vitro-stabilized-skin-model-and-in-vivo-pre-vascularized-skin-patch-using-tissue-specific-extracellular-matrix-bioink-a-step-towards-advanced-skin-tissue-engineering
#17
Byoung Soo Kim, Yang Woo Kwon, Jeong-Sik Kong, Gyu Tae Park, Ge Gao, Wonil Han, Moon-Bum Kim, Hyungseok Lee, Jae Ho Kim, Dong-Woo Cho
3D cell-printing technique has been under spotlight as an appealing biofabrication platform due to its ability to precisely pattern living cells in pre-defined spatial locations. In skin tissue engineering, a major remaining challenge is to seek for a suitable source of bioink capable of supporting and stimulating printed cells for tissue development. However, current bioinks for skin printing rely on homogeneous biomaterials, which has several shortcomings such as insufficient mechanical properties and recapitulation of microenvironment...
June 2018: Biomaterials
https://www.readbyqxmd.com/read/29603400/a-guide-to-studying-human-dermal-adipocytes-in-situ
#18
REVIEW
Carina Nicu, Jennifer Pople, Laura Bonsell, Ranjit Bhogal, David M Ansell, Ralf Paus
Dermal white adipose tissue (DWAT) is a main component of human skin, composed of individual lipid-laden mesenchymal cells known as dermal adipocytes (DAs). Besides their well-known role in lipid storage and release, DAs also promote skin immunity, wound healing and hair follicle cycling and are important players in cutaneous neuroendocrinology. The ever-growing insights into DWAT functions, albeit mostly in mice, have invited speculation that it may be involved in multiple skin diseases ranging from fibrosis to alopecia and psoriasis, thus designating human DWAT a clinically relevant, but as yet insufficiently investigated skin compartment...
June 2018: Experimental Dermatology
https://www.readbyqxmd.com/read/29559910/liposome-encapsulated-baicalein-suppressed-lipogenesis-and-extracellular-matrix-formation-in-hs68-human-dermal-fibroblasts
#19
Chien-Liang Fang, Yiwei Wang, Kevin H-Y Tsai, Hsin-I Chang
The dermis of human skin contains large numbers of fibroblasts that are responsible for the production of the extracellular matrix (ECM) that supporting skin integrity, elasticity and wound healing. Previously, an in vivo study demonstrated that dermal fibroblasts siting in the lower dermis are capable to convert into skin adipose layer and hence fibroblast lipogenesis may vary the structure and elasticity of dermis. In the present study, Hs68 human dermal fibroblasts were utilized as an in vitro model to study the lipogenesis via using adipogenic differentiation medium (ADM)...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29549768/production-scale-fibronectin-nanofibers-promote-wound-closure-and-tissue-repair-in-a-dermal-mouse-model
#20
Christophe O Chantre, Patrick H Campbell, Holly M Golecki, Adrian T Buganza, Andrew K Capulli, Leila F Deravi, Stephanie Dauth, Sean P Sheehy, Jeffrey A Paten, Karl Gledhill, Yanne S Doucet, Hasan E Abaci, Seungkuk Ahn, Benjamin D Pope, Jeffrey W Ruberti, Simon P Hoerstrup, Angela M Christiano, Kevin Kit Parker
Wounds in the fetus can heal without scarring. Consequently, biomaterials that attempt to recapitulate the biophysical and biochemical properties of fetal skin have emerged as promising pro-regenerative strategies. The extracellular matrix (ECM) protein fibronectin (Fn) in particular is believed to play a crucial role in directing this regenerative phenotype. Accordingly, Fn has been implicated in numerous wound healing studies, yet remains untested in its fibrillar conformation as found in fetal skin. Here, we show that high extensional (∼1...
June 2018: Biomaterials
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