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recombinant oxidative stress

Weiqin Yin, Yongwei Jiang, Shiqing Xu, Zai Wang, Liang Peng, Qing Fang, Tingting Deng, Wanni Zhao, Wenjian Zhang, Jinning Lou
AIMS/INTRODUCTION: Blockade or reversal the progression of diabetic nephropathy is a clinical challenge. To study whether the recombinant human glucagon-like peptide-1 have an effect on alleviate urinary protein and urinary albumin levels in diabetic rats or not. MATERIALS AND METHODS: STZ induced Diabetes Rats were treated with rhGLP-1, Insulin, and Saline. Using Immunostaining, H&E, EM and PAS staining to study the pathology of DN, and we performed qRT-PCR, Western blot and IHC to identify the differentially expressed proteins...
October 11, 2018: Journal of Diabetes Investigation
Ning Xu, Yanli Chu, Hongli Chen, Xingxing Li, Qi Wu, Liang Jin, Guixue Wang, Junli Huang
Plant roots are constantly exposed to a variety of abiotic stresses, and high salinity is one of the major limiting conditions that impose constraints on plant growth. In this study, we describe that OsMADS25 is required for the root growth as well as salinity tolerance, via maintaining ROS homeostasis in rice (Oryza sativa). Overexpression of OsMADS25 remarkably enhanced the primary root (PR) length and lateral root (LR) density, whereas RNAi silence of this gene reduced PR elongation significantly, with altered ROS accumulation in the root tip...
October 10, 2018: PLoS Genetics
M Sözmen, A K Devrim, Y B Kabak, T Devrim
Periostin is an extracellular matrix protein from the fasciclin family that guides cellular trafficking and extracellular matrix organization. Periostin stimulates mature cardiomyocytes to reenter the cell cycle. The molecular mechanism behind such stimulation remains to be explored. A DNA microarray technology constituting 30,429 gene-level probe sets was utilized to investigate effects of recombinant murine periostin peptide on the gene expression pattern in a rat model of isoproterenol (ISO)-induced myocardial injury...
October 10, 2018: Human & Experimental Toxicology
Asma Elmabruk, Banibrata Das, Deepthi Yedlapudi, Liping Xu, Tamara Antonio, Maarten E A Reith, Aloke K Dutta
We have developed a series of carbazole-derived compounds based on our hybrid D2/D3 agonist template to design multifunctional compounds for the symptomatic and disease-modifying treatment of Parkinson's disease (PD). The lead molecules (-)-11b, (-)-15a and (-)-15c exhibited high affinity for both D2 and D3 receptors where as in GTPγS functional assay, the compounds showed potent agonist activity at both D2 and D3 receptors (EC50 (GTPγS); D2 = 48.7 nM, D3 = 0.96 nM for 11b, D2 = 0.87 nM, D3 = 0.23 nM for 15a and D2 = 2...
October 9, 2018: ACS Chemical Neuroscience
Steven T Sizemore, Manchao Zhang, Ju Hwan Cho, Gina M Sizemore, Brian Hurwitz, Balveen Kaur, Norman L Lehman, Michael C Ostrowski, Pierre A Robe, Weili Miao, Yinsheng Wang, Arnab Chakravarti, Fen Xia
Resistance to genotoxic therapies is a primary cause of treatment failure and tumor recurrence. The underlying mechanisms that activate the DNA damage response (DDR) and allow cancer cells to escape the lethal effects of genotoxic therapies remain unclear. Here, we uncover an unexpected mechanism through which pyruvate kinase M2 (PKM2), the highly expressed PK isoform in cancer cells and a master regulator of cancer metabolic reprogramming, integrates with the DDR to directly promote DNA double-strand break (DSB) repair...
October 8, 2018: Cell Research
Eun Young Kim, Naghmeh Hassanzadeh Khayyat, Stuart E Dryer
The soluble urokinase receptor (suPAR) has been implicated in the pathogenesis of chronic kidney diseases (CKD) and may function as a circulating "permeability factor" driving primary focal and segmental glomerulosclerosis (FSGS). Here we examined the mechanisms whereby suPAR causes mobilization and increased activation of Ca2+ -permeable TRPC6 channels, which are also implicated in FSGS. Treatment of immortalized mouse podocytes with recombinant suPAR for 24 h caused a marked increase in cytosolic reactive oxygen species (ROS) that required signaling through integrins...
October 2018: Biochimica et biophysica acta. Molecular basis of disease
Zaher A Radi
Pathophysiologically, the classification of acute kidney injury (AKI) can be divided into three categories: (1) prerenal, (2) intrinsic, and (3) postrenal. Emerging evidence supports the involvement of renal tubular epithelial cells and the innate and adaptive arms of the immune system in the pathogenesis of intrinsic AKI. Pro-inflammatory damage-associated molecular patterns, pathogen-associated molecular patterns, hypoxia inducible factors, toll-like receptors, complement system, oxidative stress, adhesion molecules, cell death, resident renal dendritic cells, neutrophils, T and B lymphocytes, macrophages, natural killer T cells, cytokines, and secreted chemokines contribute to the immunopathogenesis of AKI...
October 3, 2018: Toxicologic Pathology
Haodan Zhu, Yong Wang, Yanxiu Ni, Junming Zhou, Lixiao Han, Zhengyu Yu, Aihua Mao, Dandan Wang, Hongjie Fan, Kongwang He
Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen responsible for septicemia and meningitis. The redox-sensing regulator Rex has been reported to play critical roles in the metabolism regulation, oxidative stress response, and virulence of various pathogens. In this study, we identified and characterized a Rex ortholog in the SS2 virulent strain SS2-1 that is involved in bacterial pathogenicity and stress environment susceptibility. Our data show that the Rex-knockout mutant strain Δrex exhibited impaired growth in medium with hydrogen peroxide or a low pH compared with the wildtype strain SS2-1 and the complementary strain CΔrex...
2018: Frontiers in Cellular and Infection Microbiology
Prasun K Mukherjee, James F Hurley, James T Taylor, Lorraine Puckhaber, Sylvia Lehner, Irina Druzhinina, Rainer Schumacher, Charles M Kenerley
Fungal siderophores are known to be involved in iron acquisition and storage, as well as pathogenicity of mammals and plants. As avirulent plant symbionts, Trichoderma spp. colonize roots and induce resistance responses both locally and systemically. To study the role of intracellular siderophore(s) in Trichoderma-plant interactions, we have obtained mutants in a non-ribosomal peptide synthetase, TvTex10, that was predicted to be involved in intracellular siderophore(s) biosynthesis. This gene has a detectable basal level of expression and is also upregulated under iron-deplete conditions...
September 29, 2018: Biochemical and Biophysical Research Communications
Yusuke Nomura, Chie Fukui, Yuki Morishita, Yuji Haishima
Introduction: Multipotent mesenchymal stem cells (MSCs) are widespread in adult organisms and are implicated in tissue maintenance and repair, regulation of hematopoiesis, and immunologic responses. Human (h)MSCs have applications in tissue engineering, cell-based therapy, and medical devices but it is unclear how they respond to unfavorable conditions such as hypoxia or inflammation after in vivo transplantation. Although endotoxin testing is a requirement for evaluating the quality and safety of transplanted MSCs, there have been no reports on the dose response to endotoxins to establish limits for in vitro MSC culture systems...
December 2017: Regenerative Therapy
Karin Koch, Emilia Strandback, Shalinee Jha, Gesa Richter, Benjamin Bourgeois, Tobias Madl, Peter Macheroux
The genome of the yeast Saccharomyces cerevisiae encodes a canonical lipoamide dehydrogenase (Lpd1p) as part of the pyruvate dehydrogenase complex and a highly similar protein termed Irc15p (increased recombination centers 15). In contrast to Lpd1p, Irc15p lacks a pair of redox active cysteine residues required for the reduction of lipoamide and thus it is very unlikely that Irc15p performs a similar dithiol-disulfide exchange reaction as reported for lipoamide dehydrogenases. We expressed IRC15 in Escherichia coli and purified the produced protein to conduct a detailed biochemical characterization...
September 29, 2018: Protein Science: a Publication of the Protein Society
Beihui Liu, Valentina Mosienko, Barbara Vaccari Cardoso, Daria Prokudina, Mathew Huentelman, Anja G Teschemacher, Sergey Kasparov
Discovery of neuroprotective pathways is one of the major priorities for neuroscience. Astrocytes are natural neuroprotectors and it is likely that brain resilience can be enhanced by mobilizing their protective potential. Among G-protein coupled receptors expressed by astrocytes, two highly related receptors, GPR37L1 and GPR37, are of particular interest. Previous studies suggested that these receptors are activated by a peptide Saposin C and its neuroactive fragments (prosaptide TX14(A)), which were demonstrated to be neuroprotective in various animal models by several groups...
September 27, 2018: Glia
Carla Letizia Busceti, Luisa Di Menna, Franca Bianchi, Federica Mastroiacovo, Paola Di Pietro, Anna Traficante, Giovanna Bozza, Christof Niehrs, Giuseppe Battaglia, Valeria Bruno, Francesco Fornai, Massimo Volpe, Speranza Rubattu, Ferdinando Nicoletti
Dickkopf-3 (Dkk3) is an atypical member of the Dkk family of Wnt inhibitors, which has been implicated in the pathophysiology of neurodegenerative disorders. However, the role of Dkk3 in mechanisms of cell degeneration and protection is unknown. We used Dkk3 knockout mice to examine how endogenous Dkk3 influences ischemic brain damage. In addition, we used primary cultures of astrocytes or mixed cultures of astrocytes and neurons to investigate the action of Dkk3 on cell damage and dissect the underlying molecular mechanisms...
2018: Frontiers in Cellular Neuroscience
Elena Lima-Cabello, Sonia Morales-Santana, Josefa León, Victor Alché, Alfonso Clemente, Juan D Alché, Jose C Jimenez-Lopez
Insulin resistance (IR) is the main contributor to the development of type 2 diabetes. In this study, we have purified recombinant β-conglutin proteins (rβ1 to rβ4, and rβ6) from narrow-leafed lupin (NLL) by using affinity chromatography. The objective of this study was to evaluate the capacity of these β-conglutins to improve the IR state using ex vivo and in vitro systems. rβ1, rβ3, and rβ6 produced lower levels of pro-inflammatory mediator nitric oxide (about -7-fold in all cases), up-regulated mRNA expression levels of IRS-1 (+201, +173, +192%) and Glut-4 (+286, +121, +147%), increased levels of p85-PI3K (+188, +187, +137-fold) and Glut-4 (+503, +548, +515-fold) proteins, higher phosphorylation levels of the insulin signalling pathway activator p-IRS-1 and downstream mediators such as p-Akt, p-Cbl, and p-caveolin, and improved glucose uptake in insulin resistant (IR-C) culture cells...
September 26, 2018: Food & Function
Sébastien Dumont, Natalia V Bykova, Alexia Khaou, Yasmine Besserour, Maude Dorval, Jean Rivoal
In plant cells, many stresses, including low oxygen availability, result in a higher production of reactive oxygen species (ROS) and reactive nitrogen species (RNS). These molecules can lead to redox-dependent post-translational modification of proteins Cys residues. Here, we studied the effect of different redox modifications on alcohol dehydrogenase (ADH) from Arabidopsis thaliana. ADH catalyzes the last step of the ethanol fermentation pathway used by plants to cope with energy deficiency during hypoxic stress...
2018: PloS One
Tsuneo Takenaka, Hiroyuki Kobori, Takashi Miyazaki, Hiromichi Suzuki, Akira Nishiyama, Naohito Ishii, Maho Yamashita, Matsuhiko Hayashi
AIMS: Klotho interacts with various membrane proteins, such as receptors for transforming growth factor (TGF)-β and insulin-like growth factor (IGF), to alter their function. Renal expression of klotho is diminished in diabetes. The present study examined whether exogenous klotho protein supplementation ameliorates kidney injury and renin-angiotensin system (RAS) in db/db mice. METHODS: We investigated the effects of klotho supplementation on diabetic kidney injury and RAS...
September 25, 2018: Acta Physiologica
Flávia Andressa Pidone Ribeiro, Camila Pontes, Alexandre De M V Machado, Oscar Bruna-Romero, Hananiah T Quintana, Flávia De Oliveira, José Ronnie Carvalho De Vasconcelos, Daniel Araki Ribeiro
The aim of this study was to evaluate the efficacy of vaccine using replication-deficient human recombinant Type 5 replication-defective adenoviruses (AdHu5) carrying sequences of the amastigote surface protein 2 (ASP2) (AdASP2) in mice infected with the Trypanosoma cruzi ( T cruzi) Y strain. A total of 16 A/Sn mice female were distributed into four groups, as follows (n = 4 per group): Group 1 - Control Group (CTRL); Group 2 - Infected Group (TC): animals were infected by subcutaneous route with 150 bloodstream trypomastigotes of T cruzi Y strain; Group 3 - Immunized Group (AdASP-2): animals were immunized by intramuscular injection (im) route with 50 µL of AdSP-2 (2 × 10 8 plaque forming units [pfu]/cam) at day 0; Group 4-Immunized and Infected Group (AdASP-2+TC): animals were immunized by im route with 50 µL of ASP-2 (2 × 10 8  pfu/cam) and infected by T cruzi at the same day (day 0)...
September 23, 2018: Journal of Cellular Biochemistry
Mugisha Samson, Taowei Yang, Mukama Omar, Meijuan Xu, Xian Zhang, Uliho Alphonse, Zhiming Rao
Catalase catalyzes the decomposition of hydrogen peroxide to water and oxygen. The main role of this enzyme is to prevent cell damage caused by reactive oxygen species (ROS). However, endogenous catalase is sensitive to high temperature and possesses limited activity. To satisfy requirements for this critical bottleneck, in this work, we improved the thermo-stability of a heme-catalase (KatX2) from a high oxidative stress resistance Bacillus pumilus ML413 through the construction of a disulfide bond between S286C and D289C...
December 2018: Enzyme and Microbial Technology
Kant Sangpairoj, Somjai Apisawetakan, Narin Changklungmoa, Pornanan Kueakhai, Pannigan Chaichanasak, Prasert Sobhon, Kulathida Chaithirayanon
Helminth 2-cys peroxiredoxin (Prx) is a major antioxidant enzyme that protects parasites against hydrogen peroxide-generating oxidative stress from the hosts' immune responses. This enzyme has been found in all stages of the tropical liver fluke, Fasciola gigantica. To investigate the potential of the recombinant F. gigantica Prx-2 (rFgPrx-2) as a vaccine candidate, vaccine trials in mice were carried out. In this study, the ICR mice were immunized with rFgPrx-2 combined with Freund's adjuvant and infected with F...
September 18, 2018: Experimental Parasitology
Yolanda Lorenzo, Kristiane Haug Berg, Amund Ringvold, Goran Petrovski, Morten C Moe, Andrew Collins, Bjørn Nicolaissen
PURPOSE: To examine levels of oxidative DNA base damage and expression of selected genes and proteins related to DNA damage repair in human limbal epithelium engineered ex vivo. METHODS: Cells were expanded from limbal tissue on cell culture-treated inserts in medium containing fetal bovine serum, recombinant growth factors, hormones and cholera toxin (COM) and in medium with human serum as the single growth-promoting additive (HS). Cells were analysed after two, three and four weeks in culture for DNA strand breaks and oxidized purine bases (Comet assay using the enzyme formamidopyrimidine DNA glycosylase, Fpg) and for expression of DNA repair enzymes APE1, OGG1 and Polβ by in situ hybridization (ISH) and by immunohistochemistry (IHC)...
September 21, 2018: Acta Ophthalmologica
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