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recombinant oxidative stress

Joseph Pierre Aboumsallem, Mudit Mishra, Ruhul Amin, Ilayaraja Muthuramu, Herman Kempen, Bart De Geest
BACKGROUND AND PURPOSE: Pleiotropic properties of high-density lipoproteins (HDL) may exert beneficial effects on the myocardium. The effect of recombinant HDLMilano on established heart failure was evaluated in C57BL/6 mice. EXPERIMENTAL APPROACH: Transverse aortic constriction (TAC) or sham operation was performed at the age of 14 weeks. Eight weeks later, TAC and sham mice were each randomised into three different groups. Reference groups were sacrificed at day 56 after operation for baseline analysis...
August 5, 2018: British Journal of Pharmacology
Arvind Kumar, Anurag Kirti, Hema Rajaram
The paradigm of involvement of LexA in regulation of only SOS-response in bacteria through the down-regulation of DNA repair genes was challenged in the unicellular cyanobacterium, Synechocystis PCC6803, wherein it was originally shown not to be associated with DNA repair and later also involved in management of carbon-starvation through up-regulation of C-metabolism genes. In the filamentous cyanobacterium, Anabaena sp. strain PCC7120, global stress management role for LexA and a consensus LexA-binding box (AnLexA-box) has been established using a LexA-overexpressing recombinant strain, AnlexA+ ...
July 25, 2018: Biochimica et Biophysica Acta
Manuel Navarro-Oviedo, Carmen Roncal, Agustina Salicio, Miriam Belzunce, Obdulia Rabal, Estefanía Toledo, Beatriz Zandio, Jose A Rodríguez, Jose A Páramo, Roberto Muñoz, Josune Orbe
Diabetes is an important risk factor for ischemic stroke (IS). Tissue-type plasminogen activator (tPA) has been associated with less successful revascularization and poor functional outcome in diabetes. We assessed whether a new thrombolytic strategy based on MMP10 was more effective than tPA in a murine IS model of streptozotocin (STZ)-induced diabetes. Wild-type mice were administered a single dose of streptozotocin (STZ) (180 mg/kg) to develop STZ-induced diabetes mellitus. Two weeks later, IS was induced by thrombin injection into the middle cerebral artery and the effect of recombinant MMP10 (6...
July 27, 2018: Translational Stroke Research
Elfide Gizem Kıvrak, Kıymet Kübra Yurt, Arife Ahsen Kaplan, Işınsu Alkan, Gamze Altun
Technological devices have become essential components of daily life. However, their deleterious effects on the body, particularly on the nervous system, are well known. Electromagnetic fields (EMF) have various chemical effects, including causing deterioration in large molecules in cells and imbalance in ionic equilibrium. Despite being essential for life, oxygen molecules can lead to the generation of hazardous by-products, known as reactive oxygen species (ROS), during biological reactions. These reactive oxygen species can damage cellular components such as proteins, lipids and DNA...
October 2017: Journal of microscopy and ultrastructure
F Brahmi, A Vejux, R Sghaier, A Zarrouk, T Nury, W Meddeb, L Rezig, A Namsi, K Sassi, A Yammine, I Badreddine, D Vervandier-Fasseur, K Madani, L Boulekbache-Makhlouf, B Nasser, G Lizard
Cholesterol oxidation products, also named oxysterols, can be formed either by cholesterol auto-oxidation, enzymatically or both. Among these oxysterols, 7-ketocholesterol (7KC) is mainly formed during radical attacks that take place on the carbon 7 of cholesterol. As increased levels of 7KC have been found in the tissues, plasma and/or cerebrospinal fluid of patients with major diseases, especially age related diseases (cardiovascular diseases, eye diseases, neurodegenerative diseases), some cancers, and chronic inflammatory diseases, it is suspected that 7KC, could contribute to their development...
July 11, 2018: Critical Reviews in Food Science and Nutrition
Vikas Anathy, Karolyn G Lahue, David G Chapman, Shi B Chia, Dylan T Casey, Reem Aboushousha, Jos L J van der Velden, Evan Elko, Sidra M Hoffman, David H McMillan, Jane T Jones, James D Nolin, Sarah Abdalla, Robert Schneider, David J Seward, Elle C Roberson, Matthew D Liptak, Morgan E Cousins, Kelly J Butnor, Douglas J Taatjes, Ralph C Budd, Charles G Irvin, Ye-Shih Ho, Razq Hakem, Kevin K Brown, Reiko Matsui, Markus M Bachschmid, Jose L Gomez, Naftali Kaminski, Albert van der Vliet, Yvonne M W Janssen-Heininger
Idiopathic pulmonary fibrosis is characterized by excessive deposition of collagen in the lung, leading to chronically impaired gas exchange and death1-3 . Oxidative stress is believed to be critical in this disease pathogenesis4-6 , although the exact mechanisms remain enigmatic. Protein S-glutathionylation (PSSG) is a post-translational modification of proteins that can be reversed by glutaredoxin-1 (GLRX)7 . It remains unknown whether GLRX and PSSG play a role in lung fibrosis. Here, we explored the impact of GLRX and PSSG status on the pathogenesis of pulmonary fibrosis, using lung tissues from subjects with idiopathic pulmonary fibrosis, transgenic mouse models and direct administration of recombinant Glrx to airways of mice with existing fibrosis...
July 9, 2018: Nature Medicine
Ghulam Murtaza, Adeel Siddiqui, Izhar Hussain
Cardiovascular (CV) diseases are caused by vascular dysfunction. The enhanced sensitivity to vasoconstrictors, reduced endothelium-derived vasodilators nitric oxide (NO) and prostacyclin (PGI2), and endothelium-derived hyperpolarization (EDH) indicate CV dysfunction. In recent years, recombinant human relaxin, known as serelaxin, has emerged as a new vasoactive drug that is useful in acute heart failure. First part of this review article encompasses the role of endogenous relaxin in CV homeostasis. Subsequently, vascular effects of serelaxin and the underlying modes of action in comparison to other vasodilators are discussed...
July 8, 2018: Current Protein & Peptide Science
Ji-Min Woo, Eun-Yeong Jeon, Eun-Ji Seo, Joo-Hyun Seo, Dong-Yup Lee, Young Joo Yeon, Jin-Byung Park
Baeyer-Villiger monooxygenases (BVMOs) can be used for the biosynthesis of lactones and esters from ketones. However, the BVMO-based biocatalysts are not so stable under process conditions. Thereby, this study focused on enhancing stability of the BVMO-based biocatalysts. The biotransformation of ricinoleic acid into (Z)-11-(heptanoyloxy)undec-9-enoic acid by the recombinant Escherichia coli expressing the BVMO from Pseudomonas putida and an alcohol dehydrogenase from Micrococcus luteus was used as a model system...
July 6, 2018: Scientific Reports
Martin Metz, Rebecca Torene, Sergio Kaiser, Michael T Beste, Petra Staubach, Andrea Bauer, Randolf Brehler, Janine Gericke, Martin Letzkus, Nicole Hartmann, Veit J Erpenbeck, Marcus Maurer
BACKGROUND: Omalizumab, a humanized recombinant monoclonal anti-IgE antibody proved to be effective in patients with chronic spontaneous urticaria (CSU), including severe and treatment-refractory CSU. Here we report omalizumab's effect on gene expression in skin biopsies from CSU patients enrolled in a double-blind, placebo-controlled study. METHODS: CSU patients (18-75 years) were randomized to either 300 mg omalizumab (n=20) or placebo (n=10) administered s.c...
July 5, 2018: Allergy
Qingfeng Meng, Ravi Gupta, Chul Woo Min, Jongyun Kim, Katharina Kramer, Yiming Wang, Sang-Ryeol Park, Iris Finkemeier, Sun Tae Kim
Previously, we reported a novel Magnaporthe oryzae- secreted protein MSP1, which triggers cell death and pathogen-associated molecular pattern (PAMP)-triggered immune (PTI) responses in rice. To investigate the MSP1 induced defense response in rice at the protein level, we employed a label-free quantitative proteomic approach, in parallel with flg22 treatment, which is a well-known elicitor. Exogenous application of MSP1 to rice leaves induced an oxidative burst, MAPK3/6 activation, and activation of pathogenesis-related genes (DUF26, PBZ, and PR-10)...
June 19, 2018: Journal of Proteomics
Kefeng Xu, Guodong Wang
The peroxiredoxins (Prxs) belong to a novel and evolutionarily conserved superfamily, which can protect cells from oxidative damage caused by ROS and play a vital role in immune responses. In the present study, a 995 base pairs (bp) Prx1 cDNA sequence (LjPrx1) with an open reading frame of 594 bp, which encoding 197 amino acid polypeptides was obtained from L. japonicus. Transcriptional expression analysis indicated that the LjPrx1 mRNA was ubiquitously expressed in all tissues tested, while a comparatively high expression level was detected in head-kidney and blood...
June 28, 2018: Fish & Shellfish Immunology
Shashank Shekhar, Yudhishthir Yadav, Amrendra Pratap Singh, Rashmita Pradhan, Gaurav Rajesh Desai, A B Dey, Sharmistha Dey
The oxidative stress plays a key role in Alzheimer's disease (AD) and Sirtuin (SIRT1) is potential mediator of oxidative pathway. This study explored the role of Syzygium aromaticum on SIRT1 and oxidative balance in amyloid beta induced toxicity. Anti-oxidative capacity of Syzygium aromaticum was performed in Aβ25-35 induced neurotoxicity in neuronal cells. Superoxide dismutase, Catalase and Glutathione enzyme activity were determined by the treatment of Syzygium aromaticum. Both recombinant and endogenous SIRT1 activity were performed in its presence...
June 27, 2018: Experimental Gerontology
Leila Zamani, Magnus Lundqvist, Ye Zhang, Magnus Aberg, Fredrik Edfors, Gholamreza Bidkhori, Anna Lindahl, Axel Mie, Adil Mardinoglu, Raymond Field, Richard Turner, Johan Rockberg, Veronique Chotteau
The optimization of bioprocesses for biopharmaceutical manufacturing by Chinese hamster ovary (CHO) cells can be a challenging endeavor and, today, heavily relies on empirical methods treating the bioreactor process and the cells as black boxes. Multi-omics approaches have the potential to reveal otherwise unknown characteristics of these systems and identify culture parameters to more rationally optimize the cultivation process. Here, the authors have applied both metabolomic and proteomic profiling to a perfusion process, using CHO cells for antibody production, to explore how cell biology and reactor environment change as the cell density reaches ≥200 × 106  cells mL-1 ...
June 29, 2018: Biotechnology Journal
Xiao-Hui Tu, Yu-Li Zhuo, Xiu-Yun Jiang, Hong-Li Liu, Ye Cao, Su Liu
In insects, superoxide dismutases (SODs) play a critical role in the scavenging of harmful reactive oxygen species (ROS) and protecting against oxidative stress induced by various environmental stresses. The Asiatic rice borer, Chilo suppressalis (Walker) (Lepidoptera: Crambidae), is an economically important insect pest of rice crops. In this study, a mitochondrial manganese SOD (Cs-mMnSOD) gene was characterized in C. suppressalis. The deduced Cs-mMnSOD protein has typical highly conserved features of mitochondrial manganese SODs, including four manganese binding residues, the signature DVWEHAYY peptide, and a mitochondrial-targeting sequence at the N-terminus...
June 22, 2018: Journal of Economic Entomology
Yi Yang, Anna Mah, Inn H Yuk, Parbir S Grewal, Abigail Pynn, Will Cole, Di Gao, Fan Zhang, Jia Chen, Lynn Gennaro, Christian Schöneich
Protein carbonylation is a post-translational modification referring to the occurrence of aldehydes and ketones in proteins. The current understanding of how carbonylation, in particular, metal-catalyzed carbonylation, occurs in recombinant monoclonal antibodies (mAbs) during production and storage is very limited. To facilitate investigations into mAb carbonylation, we developed a protein carbonylation assay with improved assay robustness and precision over the conventional assays. We applied this assay to investigate mAb carbonylation under production, storage, and stress conditions, and showed that iron, hydrogen peroxide, and polysorbate 20 at pharmaceutically relevant levels critically influence the extent of mAb carbonylation...
June 20, 2018: Journal of Pharmaceutical Sciences
Tilman Ziegler, Andrea Bähr, Andrea Howe, Katharina Klett, Wira Husada, Christian Weber, Karl-Ludwig Laugwitz, Christian Kupatt, Rabea Hinkel
Translations of new therapeutic options for cardiovascular disease from animal studies into a clinical setting have been hampered, in part by an improper reflection of a relevant patient population in animal models. In this study, we investigated the impact of thymosin β4 (Tβ4), which promotes collateralization and capillarization, during hypercholesterolemia, a known risk factor of coronary artery disease. Initial in vitro results highlighted an improved endothelial cell function upon Tβ4 treatment under control conditions and during hypercholesterolemic stress (scratch area [pixels]: oxidized low-density lipoprotein [oxLDL], 191,924 ± 7,717; and oxLDL + Tβ4, 105,621 ± 11,245)...
July 5, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
Rui Lu, Ting Zhang, Daijin Wu, Zhengyi He, Lei Jiang, Minya Zhou, Yong Cheng
Human copper/zinc superoxide dismutase (CuZn-SOD) and extracellular superoxide dismutase (EC-SOD) are two superoxide dismutases that scavenge reactive oxygen species (ROS). Their biological role of eliminating oxidative stress caused by excessive ROS levels in living organisms has been utilized in medical treatment, preventing skin photoaging and food preservation. In this study, we employed two sequences that encode human CuZn-SOD and EC-SOD, along with goat beta-casein 5' and 3' regulatory elements, to construct mammary gland-specific expression vectors...
June 20, 2018: Transgenic Research
Sonam Grover, Tarina Sharma, Yadvir Singh, Sakshi Kohli, Manjunath P, Aditi Singh, Torsten Semmler, Lothar H Wieler, Karsten Tedin, Nasreen Z Ehtesham, Seyed E Hasnain
The genome of Mycobacterium tuberculosis , the causal organism of tuberculosis (TB), encodes a unique protein family known as the PE/PPE/PGRS family, present exclusively in the genus Mycobacterium and nowhere else in the living kingdom, with largely unexplored functions. We describe the functional significance of the PGRS domain of Rv0297, a member of this family. In silico analyses revealed the presence of intrinsically disordered stretches and putative endoplasmic reticulum (ER) localization signals in the PGRS domain of Rv0297 (Rv0297PGRS)...
June 19, 2018: MBio
Yi-Kun Wang, Xiao-Nan Yang, Wei-Qing Liang, Yao Xiao, Qi Zhao, Xue-Rong Xiao, Frank J Gonzalez, Fei Li
To elucidate the metabolism of pazopanib, a metabolomics approach was performed based on ultra-performance liquid chromatography coupled with electrospray ionization quadrupole mass spectrometry. A total of 22 pazopanib metabolites were identified in vitro and in vivo. Among these metabolites, 17 were novel, including several cysteine adducts and aldehyde derivatives. By screening using recombinant CYPs, CYP3A4 and CYP1A2 were found to be the main forms involved in the pazopanib hydroxylation. Formation of a cysteine conjugate (M3), an aldehyde derivative (M15) and two N-oxide metabolites (M18 and M20) from pazopanib could induce the oxidative stress that may be responsible in part for pazopanib-induced hepatotoxicity...
July 23, 2018: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
Sai Zhang, Yaodong He, Biswarup Sen, Xiaohong Chen, Yunxuan Xie, Jay D Keasling, Guangyi Wang
The unicellular heterotrophic thraustochytrids are attractive candidates for commercial polyunsaturated fatty acids (PUFA) production. However, the reactive oxygen species (ROS) generated in their aerobic fermentation process often limits their PUFA titer. Yet, the specific mechanisms of ROS involvement in the crosstalk between oxidative stress and intracellular lipid synthesis remain poorly described. Metabolic engineering to improve the PUFA yield in thraustochytrids without compromising growth is an important aspect of economic feasibility...
June 2018: Metabolic Engineering Communications
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