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High resolution melting

Mei-Juan Yang, Yan-Long Hou, Xiao-Lan Yang, Chun-Xia Wang, Li-Xia Zhi, Chong-Ge You
Background Clinical evidence indicates that genetic variations may interfere with the mechanism of drug action. Recently, it has been reported that the single nucleotide polymorphisms (SNPs) of STAT4, PTPN2, PSORS1C1 and TRAF3IP2RA genes are associated with the clinical efficacy of tumor necrosis factor (TNF) inhibitors in the treatment of rheumatoid arthritis (RA) patients. Therefore, the detection of the SNPs linked with TNF inhibitor efficacy may provide an important basis for the treatment of RA. This study intended to establish molecular diagnostic methods for genotyping the linked SNPs based on high resolution melting (HRM) curve analysis...
December 4, 2018: Diagnosis
Hailong Wang, Doerthe Tetzlaff, James Buttle, Sean K Carey, Hjalmar Laudon, James P McNamara, Christopher Spence, Chris Soulsby
We assessed the hydrological implications of climate effects on vegetation phenology in northern environments by fusion of data from remote-sensing and local catchment monitoring. Studies using satellite data have shown earlier and later dates for the start (SOS) and end of growing seasons (EOS), respectively, in the Northern Hemisphere over the last 3 decades. However, estimates of the change greatly depend on the satellite data utilized. Validation with experimental data on climate-vegetation-hydrology interactions requires long-term observations of multiple variables which are rare and usually restricted to small catchments...
November 26, 2018: Science of the Total Environment
Shi-Yue Ma, Lin Liao, Ben-Jin He, Fa-Quan Lin
OBJECTIVE: To investigate the feasibility and clinical significance of high resolution melting(HRM) curve analysis to detect SLC4A1 gene D38A and K56E mutations in the patients with hereditary spherocytosis(HS). METHODS: Peripheral blood was collected from 23 cases of HS for routine tests and their genomic DNA was extracted by routine technique. Specific primers of mutation sites D38A and K56E of SLC4A1 gene were designed. The HRM method was used to analyze all the samples, and then the results of HRM were verified with DNA sequencing technology...
December 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
Arka Sarkar, Paribesh Acharyya, Ranjan Sasmal, Provas Pal, Sarit S Agasti, Kanishka Biswas
The discovery of new two-dimensional (2D) perovskite halides has created sensation recently because of their structural diversity and intriguing optical properties. The toxicity of Pb-based perovskite halides led to the development of Pb-free halides. Herein, we have demonstrated a one-pot solution-based synthesis of 2D ultrathin (∼1.78 nm) few-layer (2-4 layers) nanoplates (300-600 nm lateral dimension), nanosheets (0.6-1.5 μm), and nanocrystals of layered Cs3 Bi2 I9 by varying the reaction temperature from 110 to 180 °C...
November 26, 2018: Inorganic Chemistry
Esra Erdim, Zeynep Yücesoy Özkan, Halil Kurt, Bilge Alpaslan Kocamemi
Although Anammox process is a proven technology for sidestream nitrogen removal, the process faces challenges for mainstream applications in sewage treatment plants (STPs). The aim of this study was to investigate the effect of zero valent iron nanoparticles (nZVI) on process performance to eliminate confronts for mainstream applications. An SBR (sequencing batch reactor) system was fed with various nZVI concentrations (0.04-5000 ppb) within 310 days of operation. Ammonium (NH4 + -N) and nitrite (NO2 - -N) removal rates showed 58% increase in daily measurements and 73% increase in instant measurements...
February 15, 2019: Science of the Total Environment
Yvonne Ukamaka Ajamma, Thomas Ogao Onchuru, Daniel O Ouso, David Omondi, Daniel K Masiga, Jandouwe Villinger
BACKGROUND: Many arboviruses transmitted by mosquitoes have been implicated as causative agents of both human and animal illnesses in East Africa. Although epidemics of arboviral emerging infectious diseases have risen in frequency in recent years, the extent to which mosquitoes maintain pathogens in circulation during inter-epidemic periods is still poorly understood. This study aimed to investigate whether arboviruses may be maintained by vertical transmission via immature life stages of different mosquito vector species...
November 19, 2018: PLoS Neglected Tropical Diseases
Yoon Jeong Jang, Minseok Seo, Craig P Hersh, Sun-Ju Rhee, Yongjae Kim, Gung Pyo Lee
A non-synonymous SNP of CC-NBS-LRR was firstly mapped to confer resistance to anthracnose in watermelon. Newly proposed LRR domain harboring the SNP is evolutionary conserved in the Cucurbitaceae and Fabaceae. Anthracnose disease caused by Colletotrichum devastates many plants. Despite the importance of the disease, the mechanisms of resistance against it are poorly understood. Here, we identified a non-synonymous single-nucleotide polymorphism (SNP) located in a leucine-rich repeat domain as a marker for resistance to anthracnose race 1 in watermelon, using a combination of genetic analyses...
November 16, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
Justine Philteos, Brij S Karmur, Alireza Mansouri
Gliomas, that do not respond to alkylating agent chemotherapy, can be made more sensitive to chemotherapy through promotor mediated epigenetic silencing of the MGMT gene. MGMT is one of the important markers in glioblastomas as it not only predicts response to therapy but may also be used as an independent prognostic marker. As such, MGMT is gaining increasing traction in diagnosis, prognostication, and therapeutic decision-making for these highly malignant gliomas. Although, MGMT promotor methylation status is becoming more commonly used in neuro-oncology; this test remains imperfect...
November 15, 2018: American Journal of Clinical Oncology
Pornpat Athamanolap, Kuangwen Hsieh, And Tza-Huei Wang
In diagnosing bacterial infection, rapid bacterial identification (ID) and antimicrobial susceptibility testing (AST) are critical to clinicians in order to provide an effective treatment in a timely manner. The gold standard, culture-based approach provides both ID and antimicrobial susceptibility but requires several days of turnaround time. Especially in polymicrobial infections, where there are more than one organisms interacting collectively that can complicate the treatment. Here, we demonstrate a rapid bacterial diagnostic approach that is capable of bacterial ID/AST in heterogeneous samples within less than 4 hours by using digital PCR (dPCR) and digital high-resolution melt via microfluidic devices...
July 2018: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Christine M OrKeefe, Tza-Huei lJeffr Wang
This work presents a microfluidic Digital High-Resolution Melt platform for absolute quantitation and sensitive detection of locus-specific sequence variations on a molecule-by-molecule basis. The platform provides a facile means for assessment of hundreds to thousands of single DNA copies by digitizing template molecules in a 4096 1-nL array microfluidic device and observing the sequence-dependent fluorescence changes during temperature ramping. The analytical capability of this platform is demonstrated in several applications, such as digital assay characterization, detection and assessment of DNA methylation heterogeneity, and detection of rare biomarkers at frequencies as low as 0...
July 2018: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Fernando Rodríguez, Carla Vallejos, Víctor M Bolanos-Garcia, Diana Ponce, Nancy Unanue, Francisco Garay, Fernando Cassorla, Mariana Aracena
We report the case of a 3-year-old girl, who is the third child of nonconsanguineous parents, with short stature, hypertrophic cardiomyopathy, and mild dysmorphic features; all suggestive of Noonan syndrome. In addition, the patient presents with feeding difficulties, deep palmar and plantar creases, sparse hair, and delayed psychomotor and language development, all characteristics frequently observed in cardiofaciocutaneous syndrome. Molecular analysis of the Ras/ MAPK pathway genes using high-resolution melting curve analysis and gene sequencing revealed a de novo KRAS amino acid substitution of leucine to tryptophan at codon 53 (p...
December 2018: Journal of Pediatric Genetics
Dmitry S Tsvetkov, Pardha Saradhi Maram, Nadezhda S Tsvetkova, Andrey Yu Zuev, Alexandra Navrotsky
The formation thermodynamics of YBaCo4-xZnxO7+δ (x = 0, 1 and 3) oxides was determined by high temperature oxide melt solution calorimetry. All the studied oxides are thermodynamically metastable due to the tendency of cobalt to increase oxidation state under oxidizing conditions as well as to significant bond valence sum mismatch for Ba and Y in 114-oxides. Complex phase evolution in YBaCo4O7+δ at 350 - 400 °C upon oxygen absorption was revealed using incremental precise oxygen dosing. The calorimetric results support phase changes seen during in situ X-ray diffraction structural studies and provide high resolution measurement of the amount and energetics of oxygen absorbed by YBaCo4-xZnxO7+δ under equilibrium conditions...
November 15, 2018: Journal of Physical Chemistry. A
A Lambert, J Salleron, M Lion, M Rouyer, N Lozano, A Leroux, J L Merlin, Alexandre Harlé
Breast cancer is the leading cause of cancer-related death in women worldwide. Mutations of the PIK3CA gene are found in approximately 25% of breast carcinomas and are reported as activators of the PI3K/AKT/mTOR pathway. This study aims to compare three assays for the somatic mutation detection of PIK3CA gene in FFPE tissues of patients with breast cancer. We compared Cobas® PIK3CA Mutation Test (Roche Diagnostics, Meylan, France), PCR amplification-refractory mutation system Scorpions® (ARMS) and High-Resolution Melting PCR assay (HRM) for the detection of PIK3CA mutations...
November 13, 2018: Pathology Oncology Research: POR
Hua Cheng, Wei Song, Ren Nie, Yu-Xia Wang, Hui-Lian Li, Xiang-Sheng Jiang, Jun-Jun Wu, Cheng Chen, Qiong-You Wu
In this data article, we have designed a simple and facile protocol for copper-mediated synthesis of new 4-aryloxy-N-arylanilines under mild reaction conditions. The general information and synthetic procedures of all the target compounds were provided, and they were fully characterized by Nuclear Magnetic Resonance (NMR, including 1 H NMR and 13 C NMR), melting point measurements, and High-Resolution Mass Spectroscopy (HRMS). Furthermore, the inhibitory activities of these compounds against succinate-cytochrome c reductase (SCR) were evaluated, and the methods and procedures of enzyme inhibition experiments were also recorded in this data article...
December 2018: Data in Brief
Mehrandokht Sirous, Azar Dokht Khosravi, Mohammad Reza Tabandeh, Shokrollah Salmanzadeh, Nazanin Ahmadkhosravi, Sirus Amini
Background: The emergence of drug resistance among Mycobacterium tuberculosis (MTB) strains is a serious health concern worldwide. The development of rapid molecular diagnostic methods in recent years has a significant impact on the early detection of resistance to major anti-TB drugs in MTB isolates, which helps in employing appropriate treatment regimen and prevents the spread of drug-resistant strains. This study was designed to evaluate the efficacy of real-time PCR and high-resolution melting (HRM) curve analysis for the determination of resistance to rifampin (RIF), isoniazid (INH), and ofloxacin (OFX) in MTB isolates and to investigate their resistance-related mutations...
2018: Infection and Drug Resistance
Maruška Čuš, Veljko Vlaisavljević, Katja Repnik, Uroš Potočnik, Borut Kovačič
PURPOSE: The aim of this study was to investigate whether single nucleotide polymorphisms (SNPs) in selected genes, responsible for hormonal regulation of folliculogenesis, are associated with response to controlled ovarian hyperstimulation (COH) and clinical characteristics of women enrolled in in vitro fertilization (IVF) programs. METHODS: In a cross-sectional study, 60 (IVF) patients underwent COH by using gonadotropin-releasing hormone (GnRH) antagonist and recombinant follicle-stimulating hormone (rFSH) protocol...
November 8, 2018: Journal of Assisted Reproduction and Genetics
Norman Thieme, Karl Buchner, Lars Buttner, Olf Patzold, Jurgen Czarske
Crystal growth processes can profit from an electromagnetically driven melt flow, since controlling them allows to optimize the mass and heat transfer in the melt and thereby improves the structural and electrical properties of the grown crystals. This process optimization requires a precise understanding of magnetohydrodynamic (MHD) phenomena in crystal growth. Studying time-dependent MHD demands for a high temporal resolution combined with a long measurement duration to analyze transitional flow behavior...
November 5, 2018: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
Lei Guo, Cai Wang, Saotao Zhi, Zhu Feng, Chong Lei, Yong Zhou
This paper reports a novel micro-fluxgate sensor based on a double-layer magnetic core of a Fe⁻Co⁻B-based amorphous ribbon. The melt-spinning technique was carried out to obtain a Fe⁻Co⁻B-based amorphous ribbon composite of Fe58.1 Co24.9 B16 Si₁, and the obtained amorphous ribbon was then annealed at 595 K for 1 h to benefit soft magnetic properties. The prepared ribbon showed excellent soft magnetic behavior with a high saturated magnetic intensity (Bs ) of 1.74 T and a coercivity (Hc ) of less than 0...
November 30, 2017: Micromachines
Yohei Miyake, Jun-Ichi Adachi, Tomonari Suzuki, Kazuhiko Mishima, Ryuichiro Araki, Reina Mizuno, Ryo Nishikawa
PURPOSE: Alterations in the promoter of the telomerase reverse transcriptase (TERT) gene are a major mechanism of upregulating telomerase, which plays a crucial role in tumor development. Mutations in the TERT promoter have been observed in a subset of brain tumors, including adult gliomas and high-grade meningiomas. In pituitary adenomas (PAs), however, abnormalities in TERT are not fully understood. The present study aimed to investigate not only mutational but also methylation changes in the TERT promoter in PAs and to analyze their correlations with clinical variables...
November 3, 2018: Journal of Neuro-oncology
Chayapol Tungphatthong, Jutharat Somnuek, Thatree Phadungcharoen, Kornkanok Ingkaninan, Jessada Denduangboripant, Suchada Sukrong
In Thailand, there are three species of<i> Bacopa</i>, namely, <i>B. monnieri</i>, <i>B. caroliniana</i> and <i>B. floribunda</i>. Among these <i>Bacopa</i> species, <i>B. monnieri</i> is the only medicinal species used for the treatment of cognitive impairment and improvement of cognitive abilities because of its bioactive constituents, bacoside A and B. However, due to the similar characteristics of plants, it is difficult to differentiate among related species, resulting in confusion during identification...
November 2, 2018: Genome Génome / Conseil National de Recherches Canada
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