Aliesha Griffin, Colleen Carpenter, Jing Liu, Rosalia Paterno, Brian Grone, Kyla Hamling, Maia Moog, Matthew T Dinday, Francisco Figueroa, Mana Anvar, Chinwendu Ononuju, Tony Qu, Scott C Baraban
Genetic engineering techniques have contributed to the now widespread use of zebrafish to investigate gene function, but zebrafish-based human disease studies, and particularly for neurological disorders, are limited. Here we used CRISPR-Cas9 to generate 40 single-gene mutant zebrafish lines representing catastrophic childhood epilepsies. We evaluated larval phenotypes using electrophysiological, behavioral, neuro-anatomical, survival and pharmacological assays. Local field potential recordings (LFP) were used to screen ∼3300 larvae...
June 3, 2021: Communications Biology