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Demineralized bone matrix

M Savolainen, A Ritvanen, J Hukki, P Vuola, J Telkkä, J Leikola
Correction of calvarial defects after calvarial vault reconstruction (CVR) is challenging in craniosynostosis patients of advanced age and typically employs autologous bone. Demineralized bone matrix (DBM) is a potential alternative material for autologous bone, but its use has not been extended to correct calvarial defects. CVR patients operated at the Department of Plastic Surgery, Helsinki University Hospital, during 2008-2010 were retrospectively reviewed. Inclusion criteria of the study were CVR patients who received DBM plate, with or without bone dust, on calvarial defects and who had suitable uncovered defect on the contralateral side as control...
September 20, 2016: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
Ines Westphal, Claudia Jedelhauser, Gregor Liebsch, Arnd Wilhelmi, Attila Aszodi, Matthias Schieker
Bone tissue engineering (BTE) utilizing biomaterial scaffolds and human mesenchymal stem cells (hMSCs) is a promising approach for the treatment of bone defects. The quality of engineered tissue is crucially affected by numerous parameters including cell density and the oxygen supply. In this study, a novel oxygen-imaging sensor was introduced to monitor the oxygen distribution in three dimensional (3D) scaffolds in order to analyze a new cell-seeding strategy. Immortalized hMSCs, pre-cultured in a monolayer for 30-40% or 70-80% confluence, were used to seed demineralized bone matrix (DBM) scaffolds...
October 17, 2016: Biotechnology and Bioengineering
Zhiguo Yuan, Shuyun Liu, Chunxiang Hao, Weimin Guo, Shuang Gao, Mingjie Wang, Mingxue Chen, Zhen Sun, Yichi Xu, Yu Wang, Jiang Peng, Mei Yuan, Quan-Yi Guo
Tissue-engineered meniscus regeneration is a very promising treatment strategy for meniscus lesions. However, generating the scaffold presents a huge challenge for meniscus engineering as this has to meet particular biomechanical and biocompatibility requirements. In this study, we utilized acellular meniscus extracellular matrix (AMECM) and demineralized cancellous bone (DCB) to construct three different types of three-dimensional porous meniscus scaffold: AMECM, DCB, and AMECM/DCB, respectively. We tested the scaffolds' physicochemical characteristics and observed their interactions with meniscus fibrochondrocytes to evaluate their cytocompatibility...
December 2016: Biomaterials
Bilu Xiang, Yang Liu, Lu Xie, Qian Zhao, Ling Zhang, Xueqi Gan, Haiyang Yu
Osteoclasts demineralize and resorb bone once they attach to its surface. However, it's still unclear how the osteoclasts choose the specific sites for their attachments. It is postulated in this article that the decreased extracellular free ionized calcium concentration (Ca(2+)[e]) can provide a microenvironment for osteoclasts to recognize and then initiate the attachment process. The osteoclasts initially attach to the bone surface via integrating its integrin αvβ3 and RGD containing ligands in bone matrix...
October 7, 2016: Cell Biochemistry and Biophysics
Paolo Giannoni, Federico Villa, Cinzia Cordazzo, Luciano Zardi, Paolo Fattori, Rodolfo Quarto, Mauro Fiorini
Three different heterologous substitutes for bone regeneration, manufactured with equine-derived cortical powder (CP), cancellous chips (CC) and demineralized bone matrix granules (DBM), were compared in in vitro and in vivo settings. We tested: a commercially available bone paste (Osteoplant-Activagen™, consisting of aqueous collagenous carrier, CP, DBM; named A); a second-generation injectable paste (20 kDa polyethylene glycol/hydroxypropyl-methyl cellulose-based hydrogel, CP, DBM; B); a pre-formed bone filler (400 kDa polyethylene oxide/hydroxypropyl-methyl cellulose-based hydrogel, CP, CC, DBM; C)...
November 18, 2016: Biomaterials Science
Jiajia Shi, Jie Sun, Wen Zhang, Hui Liang, Qin Shi, Xiaoran Li, Yanyan Chen, Yan Zhuang, Jianwu Dai
The reconstruction of bone usually depends on substitute transplantation, which has drawbacks including the limited bone substitutes available, comorbidity, immune rejection, and limited endogenous bone regeneration. Here, we constructed a functionalized bone substitute by combining application of the demineralized bone matrix (DBM) and collagen-binding stromal-cell-derived factor-1α (CBD-SDF-1α). DBM was a poriferous and biodegradable bone substitute, derived from bovine bone and consisting mainly of collagen...
October 7, 2016: ACS Applied Materials & Interfaces
Nikhil D Chandradas, Shivamurthy Ravindra, Vivekananda M Rangaraju, Sheetal Jain, Shivaprasad Dasappa
AIM: To evaluate the efficacy of platelet rich fibrin (PRF) with or without bone graft [demineralized bone matrix (DBM) graft] in the treatment of intrabony defects based on clinical and radiographic parameters. MATERIALS AND METHODS: Thirty six intrabony defects in 36 patients were randomly divided into three different groups and were treated with group A (PRF with DBM) or group B (PRF alone) or group C [open flap debridement (OFD)]. Clinical parameters such as plaque index (PI), gingival index (GI), probing depth (PD), relative attachment level (RAL), and gingival recession (GR) were assessed at baseline and 9 months postoperatively; radiographic parameters such as linear bone growth (LBG) and percentage in bone fill (%BF) were calculated by using the image analysis software...
August 2016: Journal of International Society of Preventive & Community Dentistry
Daniela M Sousa, Francisco Conceição, Diana I Silva, Luís Leitão, Estrela Neto, Cecília J Alves, Inês S Alencastre, Herbert Herzog, Paulo Aguiar, Meriem Lamghari
Y1 receptor (Y1R)-signalling pathway plays a pivotal role in the regulation of bone metabolism. The lack of Y1R-signalling stimulates bone mass accretion that has been mainly attributed to Y1R disruption from bone-forming cells. Still, the involvement of Y1R-signalling in the control of bone-resorbing cells remained to be explored. Therefore, in this study we assessed the role of Y1R deficiency in osteoclast formation and resorption activity. Here we demonstrate that Y1R germline deletion (Y1R(-/-)) led to increased formation of highly multinucleated (n > 8) osteoclasts and enhanced surface area, possibly due to monocyte chemoattractant protein-1 (MCP-1) overexpression regulated by RANKL-signalling...
2016: Scientific Reports
Zhentao Man, Xiaoqing Hu, Zhenlong Liu, Hongjie Huang, Qingyang Meng, Xin Zhang, Linghui Dai, Jiying Zhang, Xin Fu, Xiaoning Duan, Chunyan Zhou, Yingfang Ao
Cartilage tissue engineering is the hotspot of cartilage repair. The allogenic chondrocytes appear to be a promising source of seed cells in cartilage tissue engineering. In this study, we aimed to transplant allogenic chondrocytes with chitosan hydrogel (CS)-demineralized bone matrix (DBM) hybrid scaffold (CS/DBM) to repair rabbit cartilage injury with one-step operation. After the CS/DBM scaffold was successfully fabricated, it showed that the porous CS filled the large pores of DBM, which improved the distribution of seed cells in the CS/DBM scaffold...
November 2016: Biomaterials
Nabil A Ebraheim, Grant S Buchanan, Xiaochen Liu, Maxwell E Cooper, Nicholas Peters, Jacob A Hessey, Jiayong Liu
OBJECTIVE: To report and evaluate the outcomes of patients undergoing definitive treatment for distal femur nonunion after initial treatment with a locking plate. METHODS: Fourteen patients who had undergone definitive treatment at an academic Level 1 trauma center from May 2007 to December 2013 for distal femur nonunion were identified from a fracture database. Thirteen of them were female; the average age was 65 years (range, 50-84 years). Ten patients had sustained their injuries in falls at ground level, and four in motor vehicle accidents...
August 2016: Orthopaedic Surgery
Mark Schallenberger, Helena Lovick, Jalane Locke, Todd Meyer, Gregory Juda
During August and September of 2013, temperature data loggers were shipped to and from an AATB accredited and FDA registered allograft tissue processing facility in Belgrade, MT (Bacterin International, Inc.) to five warm climate cities (Dallas, TX, El Paso, TX, New Orleans, LA, Phoenix, AZ, and Tampa, FL). Shipping data acquired from 72 independent shipments were analyzed to generate an assessment of temperature exposure, shipment times, and shipping event durations experienced during routine distribution...
August 25, 2016: Cell and Tissue Banking
Sompop Bencharit, Riley K Allen, Daniel Whitley
Insufficient bucco-lingual bone often limits the implant placement rendering bone and soft tissue regeneration/augmentation. The biological and clinical aspects of demineralized bone matrix (DBM) for augmentation with implant placement were reviewed and a clinical report was presented. A 38-year-old Hispanic female presented with missing a mandibular second premolar. Pre-operative cone beam computed tomography (CBCT) scans showed deficiency in the buccal bone. An endossesous dental implant and DBM allograft was placed with a custom healing abutment...
August 18, 2016: Journal of Oral Implantology
Yang Hefeng, Hu Yu, Sun Jingjing, Guo Weihua, Tian Weidong, Li Song
OBJECTIVE: The effect of treated dentin matrix (TDM) to the proliferation and osteogenesis differentiation of bone marrow mesenchymal stem cells (BMSCs) is evaluated in vitro. METHODS: TDM leaching solution was prepared by dentine particles suffering from gradient demineralization. Human BMSCs were isolated and cultivated, and subsequently cultivated in the TDM leaching solution. The proliferation of BMSCs was detected by CCK-8. The osteogenesis-related proteins, including collagen type I (Col I) and runt-related transcription factor-2 (Runx2), were extracted and detected by Western blot after a 7-day culture...
June 2016: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
Amanda LoGuidice, Alison Houlihan, Robert Deans
Multipotent adult progenitor cells are a recently described population of stem cells derived from the bone marrow stroma. Research has demonstrated the potential of multipotent adult progenitor cells for treating ischemic injury and cardiovascular repair; however, understanding of multipotent adult progenitor cells in orthopedic applications remains limited. In this study, we evaluate the osteogenic and angiogenic capacity of multipotent adult progenitor cells, both in vitro and loaded onto demineralized bone matrix in vivo, with comparison to mesenchymal stem cells, as the current standard...
January 2016: Journal of Tissue Engineering
Rudy U Rodriguez, Nathan Kemper, Erick Breathwaite, Sucharita M Dutta, Erin L Hsu, Wellington K Hsu, Michael P Francis
INTRODUCTION: Bone repair frequently requires time-consuming implant construction, particularly when using un-formed implants with poor handling properties. We therefore developed osteoinductive, micro-fibrous surface patterned demineralized bone matrix (DBM) fibers for engineering both defect-matched and general three-dimensional implants. METHODS AND RESULTS: Implant molds were filled with demineralized human cortical bone fibers there were compressed and lyophilized, forming mechanically strong shaped DBM scaffolds...
2016: Biofabrication
Franca N Alaribe, Mapula Razwinani, Makwese J Maepa, Felicity Bierman, Shirley C K M Motaung
BACKGROUND: Despite previous confirmation of osteoinductive potential of demineralized bone matrix (DBM) by other researchers, there is not yet any evidence of studies showing the osteoinductive activity of DBM products from South Africa tissue banks using both in vitro and animal models. This work evaluated the osteoinductivity of DBM both in vitro and in vivo. METHOD: DBM particles from six donors from the Centre for Tissue Engineering and C2C12 were cultured (5x104) in 24-well plates using DMEM/F-12 medium supplemented with 10% FBS...
July 25, 2016: Iranian Biomedical Journal
Dae Hwan Kim, Nam Lee, Dong Ah Shin, Seong Yi, Keung Nyun Kim, Yoon Ha
OBJECTIVE: To compare the fusion rate of a hydroxyapatite demineralized bone matrix (DBM) with post-laminectomy acquired autograft in lumbar interbody fusion surgery and to evaluate the correlation between fusion rate and clinical outcome. METHODS: From January 2013 to April 2014, 98 patients underwent lumbar interbody fusion surgery with hydroxyapatite DBM (HA-DBM group) in our institute. Of those patients, 65 received complete CT scans for 12 months postoperatively in order to evaluate fusion status...
July 2016: Journal of Korean Neurosurgical Society
Jin-Woo Nam, Moon-Young Kim, Se-Jin Han
BACKGROUND: The objective of this study was to place bone graft materials in cranial defects in a rabbit model and compare their bone regenerating ability according to the size and density of demineralized dentin matrix (DDM). METHODS: We selected nine healthy male rabbits that were raised under the same conditions and that weighed about 3 kg. Two circular defects 8 mm in diameter were created in each side of the cranium. The defects were grafted with DDM using four different particle sizes and densities: 0...
December 2016: Maxillofacial Plastic and Reconstructive Surgery
Davy Aubeux, Laurent Beck, Pierre Weiss, Jérome Guicheux, Bénédicte Enkel, Fabienne Pérez, Stéphane Simon
INTRODUCTION: The dentin extracellular matrix is a reservoir of bioactive molecules sequestered into dentin during dental initial development. They can be released under pathological conditions but also by controlled demineralization with bioactive materials. The purpose of this study was to investigate the ability of a biomedical hydrogel to extract and release these proteins from smashed dentin. METHODS: Smashed dentin was obtained with 2 different kinds of grinders: a blade mill and a zirconia mortar grinder...
September 2016: Journal of Endodontics
Fiona Linnea Bach-Gansmo, Nina Kølln Wittig, Annemarie Brüel, Jesper Skovhus Thomsen, Henrik Birkedal
The ability of osteocytes to demineralize the perilacunar matrix, osteocytic osteolysis, and thereby participate directly in bone metabolism, is an aspect of osteocyte biology that has received increasing attention during the last couple of years. The aim of the present work was to investigate whether osteocyte lacunar properties change during immobilization and subsequent recovery. A rat cortical bone model with negligible Haversian remodeling effects was used, with temporary immobilization of one hindlimb induced by botulinum toxin...
October 2016: Bone
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