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https://www.readbyqxmd.com/read/30120837/comparative-analysis-of-immune-cell-subsets-in-peripheral-blood-from-patients-with-periodontal-disease-and-healthy-controls
#1
Wan-Chien Cheng, Fahd Saleh, Belkais Abuaisha Karim, Francis J Hughes, Leonie S Taams
Periodontitis is a chronic inflammatory disease caused by the colonisation of teeth by the bacterial plaque biofilm and the resultant host immune responses in adjacent periodontal tissues. Disease severity can vary dramatically between patients with periodontitis, with some subjects displaying inflammation without bony destruction (gingivitis), whilst others experience chronic progressive or rapidly aggressive gingival connective tissue damage and bone loss. To determine whether peripheral immune dysregulation is associated with periodontitis, we performed extensive analysis of immune cell subsets in peripheral blood from patients with chronic or aggressive periodontitis vs...
August 18, 2018: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/30107503/plasma-indoleamine-2-3-dioxygenase-activity-is-associated-with-the-size-of-hiv-reservoir-in-patients-receiving-antiretroviral-therapy
#2
Jun Chen, Jingna Xun, Junyang Yang, Yongjia Ji, Li Liu, Tangkai Qi, Zhenyan Wang, Renfang Zhang, Yinzhong Shen, Rosalie Ponte, Vikram Mehraj, Jean-Pierre Routy, Hongzhou Lu
Background: Indoleamine 2, 3-dioxygenase (IDO) is an immunoregulatory enzyme that metabolises tryptophan to immunosuppressive kynurenines. We investigated whether IDO activity is associated with the size of HIV reservoirs. Methods: In this cross-sectional study, total HIV DNA in PBMCs from 127 HIV-infected patients receiving antiretroviral therapy (ART) was quantified. Tryptophan and kynurenine concentrations, as well as microbial translocation markers were measured in plasma samples...
August 10, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/30085299/enhanced-th1-th2-mixed-immune-responses-elicited-by-polyethyleneimine-adjuvanted-influenza-a-h7n9-antigen-ha1-2-in-chickens
#3
Li Song, Dan Xiong, Maozhi Hu, Xinan Jiao, Zhiming Pan
Influenza A (H7N9) viruses have caused severe human infections and deaths every year in China since 2013. To reduce the risk of human infection and prevent a new influenza pandemic, there is a pressing need to develop safe and effective anti-H7N9 vaccines for poultry. Polyethyleneimine (PEI) is an organic polycation used extensively as a transfection reagent for decades. Although the adjuvant potential of PEI is well studied in mammals, its applicability and immune characteristics to avian species are still very rare...
August 1, 2018: Poultry Science
https://www.readbyqxmd.com/read/30082322/subsets-of-cd4-cd8-and-cd25-hi-lymphocytes-are-in-general-not-influenced-by-isolation-and-long-term-cryopreservation
#4
Andrea Tompa, Anette Nilsson-Bowers, Maria Faresjö
Several key factors can affect the outcome of immunological studies; isolation/cryopreservation can possibly alter T, B, NK, and T-regulatory (Treg) cell marker expression patterns. Blood samples from 50 blood donors supplemented with Na-heparin or K2 EDTA were handled within 4 and 24 h after blood sampling. PBMC were isolated with different density gradients. Flow cytometric analysis of intracellular and extracellular CD markers was performed on blood samples freshly isolated PBMC, and PBMC was thawed 6 and 12 mo post-cryopreservation for the purpose of identifying B, NK, Th, T-cytotoxic, and Treg cells...
August 6, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/30059139/human-mait-cells-are-rapidly-activated-by-aspergillus-spp-in-an-apc-dependent-manner
#5
Susanne Jahreis, Sarah Böttcher, Susann Hartung, Tobias Rachow, Silke Rummler, Anna-Maria Dietl, Hubertus Haas, Grit Walther, Andreas Hochhaus, Marie von Lilienfeld-Toal
Mucosal associated invariant T cells (MAIT cells) are innate-like T cells (TC) which are known to be activated by several bacteria and viruses. However, activation of MAIT cells by moulds, such as the opportunistic human pathogen Aspergillus, is not well described. Stimulation of human PBMC with A. fumigatus, A. flavus, or A. terreus conidia revealed that in contrast to conventional CD4+ and CD8+ TC, MAIT cells responded already after 4 h of coincubation with upregulation of CD69. Furthermore, concurrent increase of CD107a expression and reduced intracellular expression of cytolytic proteins like perforin and granzyme indicated degranulation of intracellular vesicles...
July 30, 2018: European Journal of Immunology
https://www.readbyqxmd.com/read/30022471/functional-status-of-immune-cells-in-patients-with-long-lasting-type-2-diabetes-mellitus
#6
H W Nam, Y J Cho, J A Lim, S J Kim, H Kim, S Y Sim, D G Lim
Although patients with diabetes contract infectious diseases at higher frequencies, and in more severe forms, compared to non-diabetics, the underlying defects of the immune function have not been clearly defined. To address this, we designed an immune monitoring protocol and analyzed the functional status of various immune cells. Peripheral blood mononuclear cells (PBMCs) were stimulated with the proper ligands and the functional reactivity of each lineage of cells was subsequently measured. Patients with type 2 diabetes mellitus (T2DM) had PBMC composition ratios comparable to healthy controls, except for a higher frequency of B cell and effector T cell fractions...
July 19, 2018: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/30009185/intralymphatic-glutamic-acid-decarboxylase-alum-administration-induced-th2-like-specific-immunomodulation-in-responder-patients-a-pilot-clinical-trial-in-type-1-diabetes
#7
Beatriz Tavira, Hugo Barcenilla, Jeannette Wahlberg, Peter Achenbach, Johnny Ludvigsson, Rosaura Casas
GAD-alum given into lymph nodes to type 1 diabetes patients participating in an open-label pilot trial resulted in preservation of C-peptide similar to promising results from other trials. Here, we compared the immunomodulatory effect of giving GAD-alum directly into lymph nodes versus that induced by subcutaneous administration. Samples from T1D patients ( n = 6) who received 4  μ g GAD-alum into lymph nodes (LNs), followed by two booster injections one month apart, and from patients ( n = 6) who received two subcutaneous injections (SC) (20  μ g) given one month apart were compared...
2018: Journal of Diabetes Research
https://www.readbyqxmd.com/read/29978730/immunosuppression-of-human-adipose-derived-stem-cells-on-t-cell-subsets-via-the-reduction-of-nf-kappab-activation-mediated-by-pd-l1-pd-1-and-gal-9-tim-3-pathways
#8
Kaijian Zhou, Shu Guo, Shuang Tong, Qiang Sun, Fei Li, Xiaowei Zhang, Ying Qiao, Guoxin Liang
Adipose-derived stem cells (ADSCs) are a type of multipotent mesenchymal stem cells (MSCs) with immunosuppressive capacities. However, the underlying mechanisms involved in the inhibitory effects of ADSCs on T cells are not completely elucidated. In this study, human peripheral blood mononuclear cells (PBMCs) stimulated with anti-CD3/CD28 antibody-coated beads were cultured with or without allogeneic ADSCs (ADSC-to-PBMC ratio, 1:5). Surface marker levels, violet-labeled cell proliferation, apoptosis, interferon-gamma (IFN-gamma) production, and nuclear factor-kappaB (NF-kappaB) phosphorylation of CD4<sup>+</sup> and CD8<sup>+</sup> T cells were detected using flow cytometry...
July 6, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29974672/high-background-in-elispot-assays-is-associated-with-elevated-levels-of-immune-activation-in-hiv-1-seronegative-individuals-in-nairobi
#9
Amy Y Liu, Stephen C De Rosa, Brandon L Guthrie, Robert Y Choi, Rose Kerubo-Bosire, Barbra A Richardson, James Kiarie, Carey Farquhar, Barbara Lohman-Payne
INTRODUCTION: Spontaneous interferon-γ (IFNγ) released detected by enzyme-linked immunospot (ELISpot) assays may be a biological phenomenon. Markers of immune activation levels were assessed as correlates of high background among individuals in Kenya. METHODS: Couples concordantly seronegative for HIV-1 were enrolled. IFN-γ ELISpot assays were conducted and negative control wells were categorized as having either high or low background (≥50 and <50 SFU/106 peripheral blood mononuclear cells [PBMC], respectively)...
July 4, 2018: Immunity, Inflammation and Disease
https://www.readbyqxmd.com/read/29956186/utilization-of-feline-elispot-to-evaluate-the-immunogenicity-of-a-t-cell-based-fiv-map-vaccine
#10
Bikash Sahay, Alek M Aranyos, Andrew McAvoy, Janet K Yamamoto
The prototype and the commercial dual-subtype feline immunodeficiency virus (FIV) vaccines conferred protection against homologous FIV strains as well as heterologous FIV strains from the vaccine subtypes with closely related envelope (Env) sequences. Such protection was mediated by the FIV neutralizing antibodies (NAbs) induced by the vaccines. Remarkably, both prototype and commercial FIV vaccines also conferred protection against heterologous FIV subtypes with highly divergent Env sequences from the vaccine strains...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29950349/therapeutic-immune-modulation-against-solid-cancers-with-intratumoral-poly-iclc-a-pilot-trial
#11
Chrisann Kyi, Vladimir Roudko, Rachel Sabado, Yvonne M Saenger, William Loging, John Mandeli, Tin Htwe Thin, Michael Donovan, Deborah Lehrer, Marshall R Posner, Krzysztof Misiukiewicz, Benjamin Greenbaum, Andres M Salazar, Philip Friedlander, Nina Bhardwaj
PURPOSE: Polyinosinic-polycytidylic acid-poly-l-lysine carboxymethylcellulose (Poly-ICLC), a synthetic double-stranded RNA complex, is a ligand for toll-like receptor-3 (TLR3) and MDA-5 that can activate immune cells such as dendritic cells and trigger NK cells to kill tumor cells. EXPERIMENTAL DESIGN: In this pilot study, eligible patients included those with recurrent metastatic disease who failed prior systemic therapy (head and neck squamous cell cancer (HNSCC), melanoma)...
June 27, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29913200/increased-t-cell-immunosenescence-and-accelerated-maturation-phenotypes-in-older-kidney-transplant-recipients
#12
J M Schaenman, M Rossetti, T Sidwell, V Groysberg, G Sunga, Y Korin, E Liang, X Zhou, B Abdallah, E Lum, S Bunnapradist, T Pham, G Danovitch, E F Reed
Older kidney transplant recipients experience increased rates of infection and death, and less rejection, compared with younger patients. However, little is known about immune dysfunction in older compared with younger kidney transplant recipients and whether it is associated with infection. We evaluated T cell phenotypes including maturation, immune senescence, and exhaustion in a novel investigation into differences in older compared with younger patients receiving identical immune suppression regimens. We evaluated PBMC from 60 kidney transplant recipients (23 older and 37 matched younger patients) by multiparameter immune phenotyping...
June 15, 2018: Human Immunology
https://www.readbyqxmd.com/read/29866746/development-of-mgd007-a-gpa33-x-cd3-bispecific-dart-protein-for-t-cell-immunotherapy-of-metastatic-colorectal-cancer
#13
Paul A Moore, Kalpana Shah, Yinhua Yang, Ralph Alderson, Penny Roberts, Vatana Long, Daorong Liu, Jonathan C Li, Steve Burke, Valentina Ciccarone, Hua Li, Claudia B Fieger, Jeff Hooley, Ann Easton, Monica Licea, Sergey Gorlatov, Kathy L King, Peter Young, Arash Adami, Deryk Loo, Gurunadh R Chichili, Liqin Liu, Douglas H Smith, Jennifer G Brown, Francine Z Chen, Scott Koenig, Jennie Mather, Ezio Bonvini, Syd Johnson
We have developed MGD007 (anti-glycoprotein A33 x anti-CD3), a DART protein designed to redirect T cells to target gpA33 expressing colon cancer. The gpA33 target was selected on the basis of an antibody-based screen to identify cancer antigens universally expressed in both primary and metastatic colorectal cancer specimens, including putative cancer stem cell populations. MGD007 displays the anticipated-bispecific binding properties and mediates potent lysis of gpA33-positive cancer cell lines, including models of colorectal cancer stem cells, through recruitment of T cells...
August 2018: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/29807688/baboon-cd8-t-cells-suppress-sivmac-infection-in-cd4-t-cells-through-contact-dependent-production-of-mip-1%C3%AE-mip-1%C3%AE-and-rantes
#14
Veronica Obregon-Perko, Vida L Hodara, Laura M Parodi, Luis D Giavedoni
Simian immunodeficiency virus (SIV) infection in rhesus macaques is often characterized by high viremia and CD4 T cell depletion. By contrast, SIV infection in African nonhuman primate natural hosts is typically nonpathogenic despite active viral replication. Baboons are abundant in Africa and have a geographical distribution that overlaps with natural hosts, but they do not harbor SIVs. Previous work has demonstrated baboons are resistant to chronic SIV infection and/or disease in vivo but the underlying mechanisms remain unknown...
May 26, 2018: Cytokine
https://www.readbyqxmd.com/read/29783767/direct-detection-of-t-and-b-memory-lymphocytes-by-immunospot%C3%A2-assays-reveals-hcmv-exposure-that-serum-antibodies-fail-to-identify
#15
Fredrik Terlutter, Richard Caspell, Tobias M Nowacki, Alexander Lehmann, Ruliang Li, Ting Zhang, Anna Przybyla, Stefanie Kuerten, Paul V Lehmann
It is essential to identify donors who have not been infected with human cytomegalovirus (HCMV) in order to avoid transmission of HCMV to recipients of blood transfusions or organ transplants. In the present study, we tested the reliability of seronegativity as an indicator for the lack of HCMV exposure in healthy human blood donors. Eighty-two HCMV seronegative individuals were identified, and their peripheral blood mononuclear cells (PBMC) were tested in ImmunoSpot® assays for the presence of HCMV-specific T- and B-memory lymphocytes...
May 19, 2018: Cells
https://www.readbyqxmd.com/read/29755666/impact-of-lenalidomide-maintenance-on-the-immune-environment-of-multiple-myeloma-patients-with-low-tumor-burden-after-autologous-stem-cell-transplantation
#16
Karel Fostier, Jo Caers, Nathalie Meuleman, Katrijn Broos, Jurgen Corthals, Kris Thielemans, Rik Schots, Brenda De Keersmaecker
Lenalidomide is a potent anti-myeloma drug with immunomodulatory properties. It is increasingly used in a low-dose maintenance setting to prolong remission duration after standard treatment. Data on the in vivo effects of lenalidomide are scarce and sometimes different from the well-described in vitro effects. We therefore evaluated the numerical, phenotypical and functional impact of lenalidomide maintenance on several immune cell types in a cohort of seventeen homogeneously treated myeloma patients achieving a low residual myeloma burden after a bortezomib based-induction followed by autologous stem cell transplantation...
April 17, 2018: Oncotarget
https://www.readbyqxmd.com/read/29732580/quantity-quality-and-functionality-of-peripheral-blood-cells-derived-from-residual-blood-of-different-apheresis-kits
#17
Arne Knörck, Stefanie Marx, Kim S Friedmann, Sylvia Zöphel, Lisa Lieblang, Carmen Hässig, Isabelle Müller, Jan Pilch, Urban Sester, Markus Hoth, Hermann Eichler, Martina Sester, Eva C Schwarz
BACKGROUND: Research with primary human white blood cell (WBC) subpopulations requires high quantity, quality, and functionality of peripheral blood mononuclear cells (PBMCs) as a source to further characterize cellular subpopulations such as T and B lymphocytes, monocytes, or natural killer cells. Apart from buffy coats derived from whole blood, residual blood from preparative hemapheresis kits are used as a source for PBMCs, but knowledge on the yield and functionality of cells from different devices is limited...
June 2018: Transfusion
https://www.readbyqxmd.com/read/29684636/increased-frequency-of-ccr7-cd4-t-cells-from-patients-with-primary-sj%C3%A3-gren-s-syndrome-an-indicator-of-disease-activity-rather-than-of-damage-severity
#18
Chunling Wu, Pingting Yang, Haina Liu, Weiguo Xiao, Lijuan Zhao
Expression of CCR7 on T cells has been reported to be associated with the lymphocytic migration and infiltration, which is recognized as a vital part of the pathogenesis of Primary Sjögren's syndrome (pSS). Here, we compared the expression of CCR7 on CD4+T cells between pSS patients and control groups, including healthy donors (HD) and patients with systemic lupus erythematosus (SLE) and examined correlations with disease activity and damage severity, which were evaluated by EULAR Sjögren's Syndrome Disease Activity Index (ESSDAI) and Sjogren's Syndrome Disease Damage Index (SSDDI), respectively...
April 20, 2018: Cytokine
https://www.readbyqxmd.com/read/29678226/multispectral-fluorescence-activated-cell-sorting-of-b-and-t-cell-subpopulations-from-equine-peripheral-blood
#19
Joy E Tomlinson, Bettina Wagner, M Julia B Felippe, Gerlinde R Van de Walle
Immune phenotyping of equine peripheral blood mononuclear cells (PBMC) is commonly described by single or double marker labeling, which limits complex phenotypic descriptions and subpopulation identification. Our objective was to develop a new multispectral flow cytometry protocol to identify and sort equine lymphocyte subpopulations using commercially available, pre-conjugated monoclonal antibodies to cell surface markers. Two clones of anti-equine CD8 (CVS8 and CVS21) were compared in combination with CD3...
May 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29663037/polyfunctionality-of-cd4-t-lymphocytes-is-increased-after-chemoradiotherapy-of-head-and-neck-squamous-cell-carcinoma
#20
COMPARATIVE STUDY
J Doescher, S Jeske, S E Weissinger, C Brunner, S Laban, E Bölke, T K Hoffmann, T L Whiteside, P J Schuler
BACKGROUND: For head and neck squamous cell cancer (HNSCC), standard therapy consists of surgery, radiation, and/or chemotherapy. Antineoplastic immunotherapy could be an option in an adjuvant setting and is already in palliation. A functional immune system is a prerequisite for successful immunotherapy. However, effects of the standard-of-care therapy on the patients' immune system are not fully understood. METHODS: Peripheral blood mononuclear cells (PBMC) were collected from patients with HNSCC (n = 37) and healthy controls (n = 10)...
May 2018: Strahlentherapie und Onkologie: Organ der Deutschen Röntgengesellschaft ... [et Al]
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