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https://www.readbyqxmd.com/read/30120837/comparative-analysis-of-immune-cell-subsets-in-peripheral-blood-from-patients-with-periodontal-disease-and-healthy-controls
#1
Wan-Chien Cheng, Fahd Saleh, Belkais Abuaisha Karim, Francis J Hughes, Leonie S Taams
Periodontitis is a chronic inflammatory disease caused by the colonisation of teeth by the bacterial plaque biofilm and the resultant host immune responses in adjacent periodontal tissues. Disease severity can vary dramatically between patients with periodontitis, with some subjects displaying inflammation without bony destruction (gingivitis), whilst others experience chronic progressive or rapidly aggressive gingival connective tissue damage and bone loss. To determine whether peripheral immune dysregulation is associated with periodontitis, we performed extensive analysis of immune cell subsets in peripheral blood from patients with chronic or aggressive periodontitis vs...
August 18, 2018: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/30120362/thymosin-alpha1-fc-modulates-the-immune-system-and-down-regulates-the-progression-of-melanoma-and-breast-cancer-with-a-prolonged-half-life
#2
Fanwen Wang, Tingting Yu, Heng Zheng, Xingzhen Lao
Thymosin alpha 1 (Tα1) is a biological response modifier that has been introduced into markets for treating several diseases. Given the short serum half-life of Tα1 and the rapid development of Fc fusion proteins, we used genetic engineering method to construct the recombinant plasmid to express Tα1-Fc (Fc domain of human IgG4) fusion protein. A single-factor experiment was performed with different inducers of varying concentrations for different times to get the optimal condition of induced expression. Pure proteins higher than 90...
August 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30119091/effect-of-acid-suppression-on-peripheral-t-lymphocyte-subsets-and-immunohistochemical-esophageal-mucosal-changes-in-patients-with-gastroesophageal-reflux-disease
#3
Heba Ahmed Osman, Sanaa S Aly, Hasan S Mahmoud, Eman H Ahmed, Eman M Salah Eldin, Eman A Abdelrahim, Muhammad A El Masry, Rania A Herdan, Mohammed H Hassan
BACKGROUND AND AIMS: Gastroesophageal reflux disease (GERD) is a common prevalent disease. We aimed to assess the dynamic changes in the peripheral T lymphocytes and lymphocytes infiltrating the esophageal mucosa after treatment with proton-pump inhibitor (PPI) in patients with GERD. PATIENTS AND METHODS: A total of 200 patients who presented with upper GIT symptoms were included in this prospective study. All patients were subjected to full history taking, clinical examination, and complete blood count...
August 16, 2018: Journal of Clinical Gastroenterology
https://www.readbyqxmd.com/read/30118165/immunogenicity-and-protective-efficacy-of-orally-administered-recombinant-lactobacillus-plantarum-expressing-vp2-protein-against-ibdv-in-chicken
#4
Maqsood Iram, Wen Shi, Lan Wang, Xiaona Wang, Bing Han, Haiyuan Zhao, Abbas Muhammad Nadeem, Bukhari Syed Moshin, Kausar Saima, Shah Syed Jamal, Mufti Farees Ud Din, Yigang Xu, Lijie Tang, Yijing Li
AIM: To develop an effective oral vaccine against the very virulent infectious bursal disease virus (vvIBDV), we generated two recombinant Lactobacillus plantarum strains (pPG612-VP2/LP and pPG612-T7g10-VP2/LP, which carried the T7g10 translational enhancer) that displayed the VP2 protein on the surface, and compared the humoral and cellular immune responses against vvIBDV in chickens. METHODS AND RESULTS: We genetically engineered the Lactobacillus plantarum strains pPG612-VP2/LP and pPG612-T7g10-VP2/LP constitutively expressing the VP2 protein of vvIBDV...
August 17, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/30117787/discrepant-clinical-significance-of-cd28-cd8-and-cd4-cd25-high-regulatory-t-cells-during-the-progression-of-hepatitis-b-virus-infection
#5
Xing-Hua Shen, Ping Xu, Xi Yu, Hua-Feng Song, Hui Chen, Xue-Guang Zhang, Mei-Ying Wu, Xue-Feng Wang
Accumulating evidence demonstrates that CD8+ CD28- regulatory T cells increase in chronic viral infection as well as tumorigenesis. However, it is still not clear about their characteristics in hepatitis B virus (HBV) infection. In addition, it is not understood whether this regulatory immune subset is distinct from CD4+ CD25high regulatory T cells in the aspect of impact on or relationship to the progression of HBV infection. Hence, we investigated their dynamics and compared their correlations with clinical parameters in the chronic and advanced phases of HBV infection...
August 17, 2018: Viral Immunology
https://www.readbyqxmd.com/read/30117785/metastatic-thymoma-in-the-liver-of-a-dog
#6
Valerie Wiles, Jamie Haddad, Nicole Leibman, Anne C Avery, Kelly L Hughes
A 12-y-old neutered male Portuguese Water dog was presented because of a 1-y history of persistent hyporexia, diarrhea, and recurrent pyelonephritis. Abdominal ultrasound revealed hepatic nodules and diffuse splenomegaly, and radiographs revealed a mediastinal mass. Fine-needle aspirates of the liver, spleen, and mediastinal mass were suspicious for lymphoma. Flow cytometry identified small T cells that co-expressed CD4 and CD8 at all sites, most suspicious for thymoma, but lymphoma could not be ruled out. PCR for antigen receptor rearrangements analysis identified polyclonal amplification of the T-cell receptor genes, more consistent with thymoma than lymphoma...
August 17, 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/30117062/anti-pd-1-treatment-increases-18-f-fdg-uptake-by-cancer-cells-in-a-mouse-b16f10-melanoma-model
#7
Mayu Tomita, Hironobu Yasui, Kei Higashikawa, Kohei Nakajima, Hideo Takakura, Tohru Shiga, Yuji Kuge, Mikako Ogawa
BACKGROUND: Programmed cell death 1 (PD-1) inhibitors act as immune checkpoint inhibitors and are more effective for improving survival time with less toxicity as compared with conventional chemotherapies. In anti PD-1 therapy, it is important to evaluate metabolism in the cancer microenvironment, as this helps to clarify the pathological conditions. Herein, we investigate the early effects of PD-1 therapy on 2-deoxy-2-[18 F]fluoro-D-glucose ([18 F]FDG) uptake in vivo, focusing on cell distribution and glycolysis in both cancer and immune cells...
August 16, 2018: EJNMMI Research
https://www.readbyqxmd.com/read/30117046/modulatory-effect-of-the-supplemented-copper-ion-on-in-vitro-activity-of-bovine-lactoferrin-to-murine-splenocytes-and-raw264-7-macrophages
#8
Hui-Juan Zhao, Xin-Huai Zhao
Bovine lactoferrin (LF) was supplemented with Cu2+ at three contents of 0.16, 0.32, and 0.64 mg/g LF, respectively. After then, LF and Cu-supplemented LF products were assessed for immuno-modulation in murine splenocytes and RAW264.7 macrophages, using dose levels of 10-40 μg/mL and four evaluation reflectors including stimulation index of splenocytes, T lymphocyte subpopulations, macrophage phagocytosis, and cytokine secretion. The results indicated that LF and Cu-supplemented LF products had suppression on splenocytes as well as concanavalin A (ConA)- or lipopolysaccharide-stimulated splenocytes; however, using lower Cu-supplementation content (i...
August 16, 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/30116751/distinct-changes-of-btla-and-hvem-expressions-in-circulating-cd4-and-cd8-t-cells-in-hepatocellular-carcinoma-patients
#9
Jiayu Liu, Jiaqian Li, Min He, Geng-Lin Zhang, Qiyi Zhao
BTLA/HVEM (B and T lymphocyte attenuator/herpes virus entry mediator) pathways play a critical role in T cell suppression in tumor. However, its dynamic changes in different T cell subsets in peripheral blood and their clinical significance are largely unclear in cancer patients. In the current study, we showed distinct changes of BTLA and HVEM expressions on peripheral blood CD4+ and CD8+ T cells in patients with hepatocellular carcinoma (HCC); BTLA expression were significantly upregulated on circulating CD4+ but not CD8+ T cells...
2018: Journal of Immunology Research
https://www.readbyqxmd.com/read/30116750/cd4-hi-cd8-low-double-positive-t-cells-are-associated-with-graft-rejection-in-a-nonhuman-primate-model-of-islet-transplantation
#10
Yun Jung Choi, Hi-Jung Park, Hye Jin Park, Kyeong Cheon Jung, Jae-Il Lee
Peripheral CD4/CD8 double-positive (DP) T cells are associated with autoimmune disorders, cancer, and viral infection. However, the relationship between organ transplantation and DP T cells is unclear. Here, we examined the functional characteristics of peripheral DP T cells and analyzed their significance with respect to islet graft rejection in a nonhuman primate model of islet transplantation. DP T cells were functionally equivalent to conventional CD4 and CD8 T cells in terms of helper and cytotoxic activity, respectively...
2018: Journal of Immunology Research
https://www.readbyqxmd.com/read/30116615/characterization-of-the-t-cell-repertoire-after-autologous-hsct-in-patients-with-ankylosing-spondylitis
#11
E A Komech, I V Zvyagin, M V Pogorelyy, I Z Mamedov, D A Fedorenko, Y B Lebedev
Autologous hematopoietic stem cell transplantation (HSCT), a safer type of HSCT than allogeneic HSCT, is a promising therapy for patients with severe autoimmune diseases (ADs). Despite the long history of medical practice, structural changes in the adaptive immune system as a result of autologous HSCT in patients with various types of ADs remain poorly understood. In this study, we used high-throughput sequencing to investigate the structural changes in the peripheral blood T-cell repertoire in adult patients with ankylosing spondylitis (AS) during two years after autologous HSCT...
April 2018: Acta Naturae
https://www.readbyqxmd.com/read/30116318/effects-of-vitamin-d-on-apoptosis-of-t-lymphocyte-subsets-in-neonatal-sepsis
#12
Ge Zheng, Minli Pan, Zhishu Li, Wenna Xiang, Weimin Jin
Effect of vitamin D on apoptosis of peripheral blood T-lymphocyte subsets in treatment of neonatal sepsis was investigated. A total of 150 neonatal patients with sepsis were randomly divided into vitamin D treatment group (observation group) and treatment control group, while 100 healthy newborns were selected as healthy control group. T-lymphocyte subsets were detected by flow cytometer, the levels of tumor necrosis factor-α, interleukin-1 and calcitonin were determined by double-antibody immunoluminometric assay, and the effect of vitamin D on the above indicators in the treatment of sepsis was observed...
August 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30116287/dietary-vitamin-d-3-supplementation-protects-laying-hens-against-lipopolysaccharide-induced-immunological-stress
#13
Yanqiang Geng, Qiugang Ma, Zhong Wang, Yuming Guo
Background: The effects of vitamin D on the immune function of laying hens are not well understood. This study investigated the effects of vitamin D3 (VD3 ) on laying performance and immunological functions in laying hens under Escherichia coli lipopolysaccharide (LPS) challenge. Methods: In experiment one, 360 Jinghong-1 strain layers (32 weeks) were randomly divided into four groups with six replicates per group and 15 hens per replicate. Hens were fed a basal diet supplemented with different levels of VD3 (0; 500; 1500; or 3000 IU VD3 /kg of diet) for 10 weeks to determine laying performance, egg quality, and other parameters...
2018: Nutrition & Metabolism
https://www.readbyqxmd.com/read/30114952/analysis-of-peripheral-blood-lymphocyte-subsets-in-critical-patients-at-icu-admission-a-preliminary-investigation-of-their-role-in-the-prediction-of-sepsis-during-icu-stay
#14
Antonella Frattari, Ennio Polilli, Vanessa Primiterra, Vincenzo Savini, Tamara Ursini, Giancarlo Di Iorio, Giustino Parruti
A better knowledge of factors predicting the development of sepsis in patients hospitalized in intensive care unit (ICU) might help deploy more targeted preventive and therapeutic strategies. In addition to the known clinical and demographic predictors of septic syndromes, in this study, we investigated whether measuring T and B lymphocyte subsets upon admission in the ICU may help individualize the prediction of ensuing sepsis during ICU stay. Between May 2015 and December 2016, we performed a prospective cohort study evaluating peripheral blood lymphocyte T-CD4+ (T-helper cells), T-CD8+ (cytotoxic T-cells), T-CD56 + (natural killer cells), and T-CD19+ (B-lymphocytes), using flow cytometry on blood samples collected 2 days after admission in the ICU...
January 2018: International Journal of Immunopathology and Pharmacology
https://www.readbyqxmd.com/read/30112116/st2-il-33-signaling-promotes-malignant-development-of-experimental-squamous-cell-carcinoma-by-decreasing-nk-cells-cytotoxicity-and-modulating-the-intratumoral-cell-infiltrate
#15
Nádia Ghinelli Amôr, Carine Ervolino de Oliveira, Thaís Helena Gasparoto, Vanessa Garcia Vilas Boas, Graziela Perri, Ramon Kaneno, Vanessa Soares Lara, Gustavo Pompermaier Garlet, João Santana da Silva, Gislâine A Martins, Cory Hogaboam, Karen A Cavassani, Ana Paula Campanelli
Squamous cell carcinoma (SCC) is the second most common form of skin cancer and the mechanism(s) involved in the progression of this tumor are unknown. Increases in the expression of IL-33/ST2 axis components have been demonstrated to contribute to neoplastic transformation in several tumor models and interleukin-33 is correlated with poor prognosis of patients with squamous cell carcinoma of the tongue. Based on these observations, we sought to determine the role of the IL-33/ST2 pathway during the development of SCC...
July 20, 2018: Oncotarget
https://www.readbyqxmd.com/read/30112067/effect-of-tgf-%C3%AE-1-on-blood-cd4-cd25-high-regulatory-t-cell-proliferation-and-foxp3-expression-during-non-small-cell-lung-cancer-blood-metastasis
#16
Yi Hu, Weibo Qi, Li Sun, Hui Zhou, Biliu Zhou, Zhiping Yang
Metastatic circulating tumor cells in non-small cell lung cancer (NSCLC) metastasis have been reported to be associated with an immune response. The present study aimed to provide a theoretical basis for the immunomodulatory processes during NSCLC blood metastasis. NSCLC blood and normal peripheral blood mononuclear cells (PBMCs) were collected. The quantity of cluster of differentiation (CD)4+ CD25high regulatory T (Treg) cells and the intracellular forkhead box protein 3 (Foxp3) expression in CD4+ CD25high Treg cells were determined by flow cytometry...
August 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/30111562/acute-phase-cd4-t-cell-responses-targeting-invariant-viral-regions-are-associated-with-control-of-live-attenuated-simian-immunodeficiency-virus
#17
Matthew S Sutton, Amy Ellis-Connell, Ryan V Moriarty, Alexis J Balgeman, Dane Gellerup, Gabrielle Barry, Andrea M Weiler, Thomas C Friedrich, Shelby L O'Connor
We manipulated SIVmac239Δnef, a model of MHC-independent viral control, to evaluate characteristics of effective cellular responses mounted by Mauritian cynomolgus macaques (MCMs) who express the M3 MHC haplotype that has been associated with poor control of pathogenic SIV. We created SIVΔnef-8x to test the hypothesis that effective SIV-specific T cell responses targeting invariant viral regions can emerge in the absence of immunodominant CD8+ T cell responses targeting variable epitopes, and that control is achievable in individuals lacking known 'protective' MHC alleles...
August 15, 2018: Journal of Virology
https://www.readbyqxmd.com/read/30111216/optimal-concentration-of-the-transversus-abdominis-plane-block-in-enhanced-recovery-after-surgery-protocols-for-patients-of-advanced-age-undergoing-laparoscopic-rectal-cancer-surgery
#18
Ping-Chen, Qing-Song Lin, Xian-Zhong Lin
Objective The transversus abdominis plane (TAP) block ameliorates visual analogue scale scores and decreases morphine requirements, but its role remains unclear. Patients of advanced age are susceptible to local anesthetic intoxication. We aimed to identify an optimal concentration that can be used in enhanced recovery after surgery (ERAS) without compromising analgesic efficacy. Methods In total, 120 patients aged ≥65 years undergoing laparoscopic rectal cancer surgery received general anesthesia combined with a TAP block using 0...
August 15, 2018: Journal of International Medical Research
https://www.readbyqxmd.com/read/30109436/what-role-does-metal-allergy-sensitization-play-in-total-knee-arthroplasty-revision
#19
David R Lionberger, Justin Samorajski, Charlie D Wilson, Andreana Rivera
BACKGROUND: Clinicians are often faced with the decision whether to revise a painful total knee replacement in patients who have chronic vague pain with no apparent explanation. A sensitive metal testing assay called the lymphocyte stimulation test has been used to detect nickel sensitization in patients with orthopedic implants. We hypothesize that nickel sensitization plays a role in the pathology of failed joint arthroplasty in patients with unexplained dissatisfaction. METHODS: 32 patients with symptomatic total knee arthroplasty without obvious mechanical findings were tested prior to revision surgery...
August 14, 2018: Journal of Experimental Orthopaedics
https://www.readbyqxmd.com/read/30107890/the-effects-of-bisphenol-a-on-some-plasma-cytokine-levels-and-distribution-of-cd8-and-cd4-t-lymphocytes-in-spleen-ileal-peyer-s-patch-and-bronchus-associated-lymphoid-tissue-in-rats
#20
Tuğba Özaydın, Yasemin Öznurlu, Emrah Sur, İlhami Çelik, Deniz Uluışık
The effects of bisphenol A on the some plasma cytokine levels and distribution of CD8+ and CD4+ T lymphocytes in spleen, ilealPeyer's patch and bronchus-associated lymphoid tissue in rats were investigated. A total of fourty male Wistar Albino rats were divided into five groups including 8 rats in each one: control, vehicle, BPA 5, BPA 50 and BPA 500 groups. Doses of 5, 50 and 500 μg/kg BPA were dissolved in ethanol, then mixed with corn oil. The control group received no treatment. The vehicle group was given the ethanol-corn oil mixture...
August 11, 2018: Acta Histochemica
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