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https://www.readbyqxmd.com/read/29915732/prevalent-infant-feeding-practices-among-the-mothers-presenting-at-a-tertiary-care-hospital-in-garhwal-himalayan-region-uttarakhand-india
#1
Vyas Kumar Rathaur, Monika Pathania, Charu Pannu, Anand Jain, Minakshi Dhar, Nitish Pathania, Rahul Goel
Introduction: There is paucity of studies on infant feeding practices from the rural areas of garhwal Himalayas of the state of uttarakhand. The present study was designed to assess the infant feeding practices in Garhwal region. Infant feeding practices have significant implications on a child's health. Early nutritional status especially during the first year of life has been shown to have a significant effect on child health and development. Optimal infant feeding practices are crucial for nutritional status, growth, development, health, and ultimately the survival of infants and young children...
January 2018: Journal of Family Medicine and Primary Care
https://www.readbyqxmd.com/read/29777525/preconcentration-and-determination-of-trace-nickel-and-cobalt-in-milk-based-samples-by-ultrasound-assisted-cloud-point-extraction-coupled-with-flame-atomic-absorption-spectrometry
#2
Nuket Kartal Temel, Kadriye Sertakan, Ramazan Gürkan
In this study, ultrasound-assisted cloud point extraction (UA-CPE) method was developed for the determination of Ni(II) and Co(II) in milk-based products. After extraction and preconcentration, the Ni(II) and Co(II) contents of samples were determined by flame atomic absorption spectrometry (FAAS). After their complexation with hydroxy naphthol blue (HNB) in the presence of cationic surfactant, CTAB at pH 4.0, the Ni(II) and Co(II) were taken within the micellar phase of nonionic surfactant, TX-114. The micellar phase containing the analytes were diluted to a volume of 0...
May 18, 2018: Biological Trace Element Research
https://www.readbyqxmd.com/read/29750967/evaluation-of-four-novel-isothermal-amplification-assays-towards-simple-and-rapid-genotyping-of-chloroquine-resistant-plasmodium-falciparum
#3
Madhvi Chahar, Anup Anvikar, Rajnikant Dixit, Neena Valecha
Loop mediated isothermal amplification (LAMP) assay is sensitive, prompt, high throughput and field deployable technique for nucleic acid amplification under isothermal conditions. In this study, we have developed and optimized four different visualization methods of loop-mediated isothermal amplification (LAMP) assay to detect Pfcrt K76T mutants of P. falciparum and compared their important features for one-pot in-field applications. Even though all the four tested LAMP methods could successfully detect K76T mutants of P...
July 2018: Experimental Parasitology
https://www.readbyqxmd.com/read/29698484/rapid-detection-of-puccinia-triticina-causing-leaf-rust-of-wheat-by-pcr-and-loop-mediated-isothermal-amplification
#4
C Manjunatha, Sapna Sharma, Deepika Kulshreshtha, Sangeeta Gupta, Kartar Singh, Subhash C Bhardwaj, Rashmi Aggarwal
Leaf rust of wheat caused by Puccinia triticina has significant impact on wheat production worldwide. Effective and quick detection methodologies are required to mitigate yield loss and time constraints associated with monitoring and management of leaf rust of wheat. In the present study, detection of P. triticina has been simplified by developing a rapid, reliable, efficient and visual colorimetric method i.e., loop mediated isothermal amplification of DNA (LAMP). Based on in silico analysis of P. triticina genome, PTS68, a simple sequence repeat was found highly specific to leaf rust fungus...
2018: PloS One
https://www.readbyqxmd.com/read/29537347/rapid-detection-and-subtyping-of-human-papillomaviruses-in-condyloma-acuminatum-using-loop-mediated-isothermal-amplification-with-hydroxynaphthol-blue-dye
#5
Q Zhong, K Li, D Chen, H Wang, Q Lin, W Liu
Objective Condyloma acuminatum (CA) is a common, viral, sexually transmitted disease worldwide. Human papillomavirus (HPV) genotyping has important clinical implications for the treatment of CA. We developed a loop-mediated isothermal amplification (LAMP) method for the detection of HPV. Methods We collected 294 cervical scrape samples, including 30 HPV-6-positive, 30 HPV-11-positive, 22 HPV-16-positive, 20 HPV-42-positve, 30 HPV-43-positive, 20 HPV-44-positive and 142 HPV-negative samples. Tissues from 40 patients with a pathological diagnosis of CA were paraffin-embedded and analyzed by LAMP and Luminex...
July 2018: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/29319815/chemical-analysis-and-simulated-pyrolysis-of-tobacco-heating-system-2-2-compared-to-conventional-cigarettes
#6
Xiangyu Li, Yanbo Luo, Xingyi Jiang, Hongfei Zhang, Fengpeng Zhu, Shaodong Hu, Hongwei Hou, Qingyuan Hu, Yongqiang Pang
Introduction: Tobacco Heating System 2.2 (THS 2.2, marketed as iQOS), is a heat-not-burn (HNB) tobacco product that has been successfully introduced to global markets. Despite its expanding market, few independent and systematic researches into THS 2.2 have been carried out to date. Methods: We tested a comprehensive list of total particulate matter (TPM), water, tar, nicotine, propylene glycol, glycerin, carbon monoxide, volatile organic compounds, aromatic amines, hydrogen cyanide, ammonia, N-nitrosamines, phenol, and polycyclic aromatic hydrocarbon under both ISO and HCI regimes...
January 8, 2018: Nicotine & Tobacco Research: Official Journal of the Society for Research on Nicotine and Tobacco
https://www.readbyqxmd.com/read/29291345/hierarchical-nanoboxes-composed-of-co-9-s-8-mos-2-nanosheets-as-efficient-electrocatalysts-for-the-hydrogen-evolution-reaction
#7
Vinoth Ganesan, Sunghyun Lim, Jinkwon Kim
The development of hydrogen evolution catalysts based on nonprecious metals is essential for the practical application of water-splitting devices. Herein, the synthesis of Co9 S8 -MoS2 hierarchical nanoboxes (HNBs) as efficient catalysts for the hydrogen evolution reaction (HER) is reported. The surface of the hollow cubic structure was organized by CoMoS4 nanosheets formed through the reaction of MoS4 2- and Co2+ released from the cobalt zeolite imidazole framework (ZIF-67) templates under reflux in a mixture of water/ethanol...
February 16, 2018: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/29248896/heat-not-burn-tobacco-product-use-in-japan-its-prevalence-predictors-and-perceived-symptoms-from-exposure-to-secondhand-heat-not-burn-tobacco-aerosol
#8
Takahiro Tabuchi, Silvano Gallus, Tomohiro Shinozaki, Tomoki Nakaya, Naoki Kunugita, Brian Colwell
OBJECTIVES: A heat-not-burn (HNB) tobacco product, IQOS, was first launched in Japan and Italy as test markets and is currently in commerce in 30 countries. Using two data sources, we examined interest in HNB tobacco (IQOS, Ploom and glo), its prevalence, predictors of its use and symptoms from exposure to secondhand HNB tobacco aerosol in Japan, where HNB tobacco has been sold since 2014. METHODS: Population interest in HNB tobacco was explored using Google search query data...
July 2018: Tobacco Control
https://www.readbyqxmd.com/read/29236795/comparative-study-of-intraperitoneal-adhesions-related-to-light-weight-polypropylene-mesh-and-type-i-polymerized-and-purified-bovine-collagen-coated-light-weight-polypropylene-mesh-in-rabbits
#9
Diego Paim Carvalho Garcia, Clarissa Santos, Cristiana Buzelin Nunes, Marcelo Araújo Buzelin, Andy Petroianu, Luiza Ohasi de Figueiredo, Andreia Souto da Motta, Cristiane de Barros Gaspar, Luiz Ronaldo Alberti
PURPOSE: To compare the effectiveness of light-weight polypropylene mesh coated with polymerized and purified bovine type I collagen (Surgidry HNB) in the treatment of abdominal wall defect and the degree of adhesion formation. METHODS: Two types of polypropylene mesh were implanted after creation of defect measuring 6.0cm X 5.5cm in the anterior abdominal wall of 32 male New Zealand breed rabbits, divided in two groups (n = 32): (1) light-weigh macroporous polypropylene, (2) type I polymerized and purified bovine collagen coated light-weigh macroporous polypropylene...
November 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29173133/the-detection-of-entamoeba-histolytica-and-toxoplasma-gondii-in-wastewater
#10
Caroline Ajonina, Christopher Buzie, Julia Möller, Ralf Otterpohl
To increase current knowledge on the epidemiology of protozoan parasites in wastewater treatment plants (WWTP), the occurrence of Entamoeba histolytica and Toxoplasma gondii in raw and treated wastewater was investigated. Samples were collected from WWTP twice a month over a period of 8 months. Determination of protozoa was performed using polymerase chain reaction (PCR) and light microscopy. After concentration and purification of wastewater samples, DNA extraction was conducted followed by PCR amplification of small subunit ribosomal RNA (SSU rRNA) gene sequences of E...
2018: Journal of Toxicology and Environmental Health. Part A
https://www.readbyqxmd.com/read/29109010/a-novel-isothermal-amplification-based-method-to-detect-mycobacterium-tuberculosis-complex
#11
Dongxin Liu, Bing Zhao, Xichao Ou, Huiwen Zheng, Aijing Ma, Wencong He, Shengfen Wang, Yang Zhou, Hui Xia, Yang Zheng, Ping Hou, Guangxue He, Yanlin Zhao
Mycobacterium tuberculosis (MTB) is the causative agent of pulmonary tuberculosis. Rapid and accurate diagnosis is crucial to tuberculosis control and prevention. A series of diagnostic methods has been available for MTB detection; however, new rapid, simple and affordable methods are needed. In this study, a multiple cross displacement amplification (MCDA)-based assay was developed to detect the IS6110 gene of the M. tuberculosis complex. Hydroxy naphthol blue (HNB), a colorimetric indicator, was used to detect amplification products...
February 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29066786/rapid-on-site-evaluation-of-the-development-of-resistance-to-quinone-outside-inhibitors-in-botrytis-cinerea
#12
X R Hu, D J Dai, H D Wang, C Q Zhang
Botrytis cinerea, a typical "high-risk" pathogenic fungus that rapidly develops resistance to fungicides, affects more than 1,000 species of 586 plant genera native to most continents and causes great economic losses. Therefore, a rapid and sensitive assay of fungicide resistance development in B. cinerea populations is crucial for scientific management. In this study, we established a Loop-mediated isothermal amplification (LAMP) system for the monitoring and evaluation of the risk of development of B...
October 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28841970/semi-quantitative-visual-detection-of-loop-mediated-isothermal-amplification-lamp-generated-dna-by-distance-based-measurement-on-a-paper-device
#13
Irin Hongwarittorrn, Nuntaree Chaichanawongsaroj, Wanida Laiwattanapaisal
A distance-based paper analytical device (dPAD) for loop mediated isothermal amplification (LAMP) detection based on distance measurement was proposed. This approach relied on visual detection by the length of colour developed on the dPAD with reference to semi-quantitative determination of the initial amount of genomic DNA. In this communication, E. coli DNA was chosen as a template DNA for LAMP reaction. In accordance with the principle, the dPAD was immobilized by polyethylenimine (PEI), which is a strong cationic polymer, in the hydrophilic channel of the paper device...
December 1, 2017: Talanta
https://www.readbyqxmd.com/read/28811820/modified-mahalanobis-taguchi-system-for-imbalance-data-classification
#14
Mahmoud El-Banna
The Mahalanobis Taguchi System (MTS) is considered one of the most promising binary classification algorithms to handle imbalance data. Unfortunately, MTS lacks a method for determining an efficient threshold for the binary classification. In this paper, a nonlinear optimization model is formulated based on minimizing the distance between MTS Receiver Operating Characteristics (ROC) curve and the theoretical optimal point named Modified Mahalanobis Taguchi System (MMTS). To validate the MMTS classification efficacy, it has been benchmarked with Support Vector Machines (SVMs), Naive Bayes (NB), Probabilistic Mahalanobis Taguchi Systems (PTM), Synthetic Minority Oversampling Technique (SMOTE), Adaptive Conformal Transformation (ACT), Kernel Boundary Alignment (KBA), Hidden Naive Bayes (HNB), and other improved Naive Bayes algorithms...
2017: Computational Intelligence and Neuroscience
https://www.readbyqxmd.com/read/28803792/rapid-and-visual-detection-of-leptospira-in-urine-by-ligb-lamp-assay-with-pre-addition-of-dye
#15
Syed Atif Ali, Gurpreet Kaur, Nongthombam Boby, T Sabarinath, Khushal Solanki, Dheeraj Pal, Pallab Chaudhuri
Leptospirosis is considered to be the most widespread zoonotic disease caused by pathogenic species of Leptospira. The present study reports a novel set of primers targeting LigB gene for visual detection of pathogenic Leptospira in urine samples through Loop-mediated isothermal amplification (LAMP). The results were recorded by using Hydroxyl napthol blue (HNB), SYBR GREEN I and calcein. Analytical sensitivity of LAMP was as few as 10 leptospiral organisms in spiked urine samples from cattle and dog. LigB gene based LAMP, termed as LigB-LAMP, was found 10 times more sensitive than conventional PCR...
December 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28637344/nicotine-delivery-to-the-aerosol-of-a-heat-not-burn-tobacco-product-comparison-with-a-tobacco-cigarette-and-e-cigarettes
#16
Konstantinos E Farsalinos, Nikoletta Yannovits, Theoni Sarri, Vassilis Voudris, Konstantinos Poulas
Introduction: The purpose was to measure nicotine levels to the tobacco and levels emitted to the aerosol of a heat-not-burn product (HnB, IQOS) compared to e-cigarettes (ECs) and a tobacco cigarette. Methods: The HnB device and regular and menthol sticks were purchased from Italy. Three types of ECs (ciga-like, eGo-style, and variable wattage) and a commercially-available tobacco cigarette were also tested. A custom-made liquid containing 2% nicotine was used with ECs...
July 9, 2018: Nicotine & Tobacco Research: Official Journal of the Society for Research on Nicotine and Tobacco
https://www.readbyqxmd.com/read/28585170/rapid-and-simple-identification-of-carbapenemase-genes-bla-ndm-bla-oxa-48-bla-vim-bla-imp-14-and-bla-kpc-groups-in-gram-negative-bacilli-by-in-house-loop-mediated-isothermal-amplification-with-hydroxynaphthol-blue-dye
#17
Arpasiri Srisrattakarn, Aroonlug Lulitanond, Chotechana Wilailuckana, Nicha Charoensri, Lumyai Wonglakorn, Pimjai Saenjamla, Prajuab Chaimanee, Jureerut Daduang, Aroonwadee Chanawong
Carbapenem-resistant Enterobacteriaceae isolates by carbapenemase production are being reported globally with increasing frequency, leading to limited therapeutic options. We therefore developed a loop-mediated isothermal amplification method with hydroxynaphthol blue dye (LAMP-HNB) for rapid confirmation of bla NDM , bla OXA-48 , bla VIM , bla IMP-14 and bla KPC groups. Sixty-two Enterobacteriaceae and Pseudomonas spp. isolates carrying various carbapenemase genes (28 bla NDM-1 , 9 bla IMP-14a , 2 bla IMP-48 , 1 bla IMP-1 , 1 bla IMP-4 , 1 bla IMP-9 , 1 bla IMP-15 , 4 bla VIM-2 , 1 bla VIM-1 , 1 bla IMP-14a & bla VIM-2 , 7 bla KPC-2 , 3 bla OXA-48 and 3 bla OXA-181 ) and 37 non-carbapenemase-producing Enterobacteriaceae isolates as confirmed by the PCR methods were included...
July 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28573341/identification-of-methicillin-resistant-staphylococcus-aureus-mrsa-using-simultaneous-detection-of-meca-nuc-and-femb-by-loop-mediated-isothermal-amplification-lamp
#18
Changguo Chen, Qiangyuan Zhao, Jianwei Guo, Yanjun Li, Qiuyuan Chen
The aim of this study was to develop a rapid detection assay to identify methicillin-resistant Staphylococcus aureus by simultaneous testing for the mecA, nuc, and femB genes using the loop-mediated isothermal amplification (LAMP) method. LAMP primers were designed using online bio-software ( http://primerexplorer.jp/e/ ), and amplification reactions were performed in an isothermal temperature bath. The products were then examined using 2% agarose gel electrophoresis. MecA, nuc, and femB were confirmed by triplex TaqMan real-time PCR...
August 2017: Current Microbiology
https://www.readbyqxmd.com/read/28319712/toxic-compounds-biodegradation-and-toxicity-of-high-strength-wastewater-treated-under-elevated-nitrogen-concentration-in-the-activated-sludge-and-membrane-bioreactor-systems
#19
Jarungwit Boonnorat, Nimaradee Boonapatcharoen, Pradthana Prachanurak, Ryo Honda, Supaporn Phanwilai
This research has assessed the removal efficiencies of toxic compounds in the high strength wastewater (the leachate and agriculture wastewater mixture) using the activated sludge (AS) and membrane bioreactor (MBR) technologies under two carbon to nitrogen (C/N) ratios (C/N 14 and 6) and two toxic compounds concentrations (8-396μg/L and 1000μg/L). In addition, the toxicity evaluations of the AS and MBR effluents to the aquatic environment were undertaken at five effluent dilution ratios (10, 20, 30, 50 and 70% v/v)...
August 15, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28109842/reverse-transcriptase-loop-mediated-isothermal-amplification-for-the-detection-of-feline-coronavirus
#20
Angelica Stranieri, Stefania Lauzi, Alessia Giordano, Saverio Paltrinieri
The Feline coronavirus (FCoV) is the etiological agent of feline infectious peritonitis (FIP), a lethal disease of felids. The role of molecular methods is controversial for the diagnosis of FIP, while essential for the identification of the shedders. Thus, a fast and inexpensive method for the detection of FCoV could be beneficial, especially in multicat environments. A reverse transcription loop mediated isothermal amplification (RT-LAMP) assay was developed. RNA extraction and RT-nPCR for FCoV were performed on thirty-two samples (11 faeces, 9 blood, 8 effusions, and 4 lymph nodes) collected from 27 cats...
May 2017: Journal of Virological Methods
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