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Egg freezing

Lucie Gavin-Plagne, Loris Commin, Pierre Bruyère, Samuel Buff, Thierry Joly
Animal-derived products are widely used in sperm cryopreservation for their cryoprotective properties. These components, however, tend to be replaced because of sanitary risks. STEMALPHA.CRYO3 (Ref. 5617; Stem Alpha, Saint-Genis-l'Argentière, France), called "CRYO3," is a chemically defined preservation medium currently used for freezing human tissue and adult stem cells. The aim of this study was to evaluate the effect of a CRYO3-based medium on ram sperm freezing regarding in vitro parameters and in vivo fertility...
October 16, 2018: Biopreservation and Biobanking
Manuel T Orrego, Sofía I Melian, Judith Montenegro, Alejandra N Cimato, Humberto Cisale, Lidia L Piehl
Increased protein tyrosine phosphorylation and the appearance of a phosphorylated protein of 32 kD (p32) are reported among the capacitation-like changes in cryopreserved boar sperm. Egg yolk freezing extenders are composed by two fractions: insoluble granules and soluble plasma, which contains the low density lipoproteins (LDL) proposed as responsible for the egg yolk cryoprotective action. The aim of this work was to analyze the effects of complete egg yolk and its insoluble, soluble and LDL fractions on boar sperm quality and protein tyrosine phosphorylation after the first stage of a standard cryopreservation protocol...
September 29, 2018: Theriogenology
M Álvarez-Rodriguez, M Álvarez, L Anel-López, C Guerra, C A Chamorro, L Anel, P de Paz, F Martínez-Pastor
Genome Resource Banks are keystones in the ex-situ conservation of wild species. Post-mortem (PM) collection of epididymal spermatozoa is an opportunistic and valuable source of germplasm, the time from the death of the animal limits its use. Seeking to improve germplasm preservation strategies for the chamois (Rupicapra sp.), the effect of PM time on epididymal sperm quality and freezability was studied using the Cantabrian chamois. Samples were classified according to PM collection time, up to 216 h (refrigerated), and cryopreserved (Tris-citric acid-fructose, 430 mOsm/kg, 15% egg yolk, 8% glycerol; freezing at -20 °C/min)...
September 29, 2018: Animal Reproduction Science
Luana Grasiele Pereira Bezerra, Ana Liza Paz Souza, Herlon Victor Rodrigues Silva, Fábio Roger Vasconcelos, Arlindo de Alencar Araripe Moura, Alexsandra Fernandes Pereira, Moacir Franco de Oliveira, Alexandre Rodrigues Silva
The aim was to describe, through scanning electron microscopy (SEM) and transmission electron microscopy (TEM), the ultrastructure of peccaries' fresh and frozen-thawed sperm. For that, semen derived from three mature males was obtained by electroejaculation and evaluated for motility, membrane integrity, membrane functionality, chromatin integrity, and morphology through light microscopy. Samples were frozen using a Tris extender plus egg yolk (20%) and glycerol (6%). Then, fresh and frozen-thawed semen samples were mixed in different sperm pools that were processed for SEM and TEM...
October 8, 2018: Microscopy Research and Technique
Guiulfo Mamani-Mango, Milagros Moina Gonzales, Martín Ramos Hidalgo, José Mendoza Mallma, Jaime Ruiz Béjar, Victoria Rivas Palma, Edwin Mellisho Salas
In alpacas, improvement of reproductive efficiency of male camelids is limited by the small testicular size, low spermatozoa production, and low quality of semen. In this study we aim to evaluate the effect of two extenders and two freezing rates on post-thaw quality of sperm recovered from alpaca epididymis with two methods (flushing and mincing), and to evaluate the in vitro fertilization (IVF) capacity of frozen sperm selected with two different selection methods (washing and swim-up). Sperm samples were processed with Tris-egg yolk or Bioxcell® extenders and frozen with slow freezing and fast freezing...
September 26, 2018: Biopreservation and Biobanking
Lisa Campo-Engelstein, Rohia Aziz, Shilpa Darivemula, Jennifer Raffaele, Rajani Bhatia, Wendy M Parker
In 2012, the American Society for Reproductive Medicine (ASRM) lifted the experimental label on oocyte preservation, but cautioned against women using it to avoid age-related infertility, known as social egg freezing (SEF). In 2014, Facebook and Apple announced that they would offer SEF as a workplace benefit. Within the context of a rapidly growing market for SEF, we were interested in how these two decisions affected media discussions, given that such discourse can strongly influence public perceptions and behaviors...
September 20, 2018: AJOB Empirical Bioethics
Benjamin P Jones, Paul Serhal, Jara Ben Nagi
We read with interest the response to our commentary on social egg freezing (SEF), by Levin et al 2018. We are encouraged to have proponents to our belief that single women should consider freezing their oocytes before the exponential decline in their ovarian reserve. We concur that there is irrefutable evidence demonstrating the clinical implications, including low response to controlled ovarian stimulation, of the exponential age related decline in ovarian reserve from the age of 37 onwards. This article is protected by copyright...
September 19, 2018: Acta Obstetricia et Gynecologica Scandinavica
Gill Lockwood, Bart Cjm Fauser
No abstract text is available yet for this article.
August 21, 2018: Reproductive Biomedicine Online
José A B Bezerra, Andréia M Silva, Patrícia C Sousa, Lívia B Campos, Érica C G Praxedes, Luana G P Bezerra, Thibério S Castelo, Ana L P Souza, Alexandre R Silva
SummaryThe aim of this study was to establish a functional freezing-thawing protocol for epididymal sperm of collared peccaries (Pecari tajacu L., 1758) by comparing different extenders. The epididymal sperm from 12 sexually mature males was recovered by retrograde flushing using Tris-based or coconut water-based (ACP®-116c) extenders. After initial evaluation, samples were diluted and frozen with the same extenders to which 20% egg yolk and 6% glycerol were added. After 2 weeks, thawing was performed at 37°C/60 s and sperm motility, vigour, morphology, functional membrane integrity, sperm viability, sperm plasma membrane integrity, and a computer-assisted semen analysis (CASA) were assessed...
September 17, 2018: Zygote: the Biology of Gametes and Early Embryos
Emily K Heying, Kaitlin Leary Ziemer, Jacob P Tanumihardjo, Natalia Palacios-Rojas, Sherry A Tanumihardjo
Background: Consumption of provitamin A carotenoid biofortified crops, such as maize, supports vitamin A (VA) status in animals and humans. Laying hens that consume β-cryptoxanthin-biofortified maize deposit β-cryptoxanthin into egg yolk. Objective: We investigated whether β-cryptoxanthin-biofortified egg consumption would affect VA status of male Mongolian gerbils (Meriones unguiculatus) compared with white-yolked eggs. Methods: β-Cryptoxanthin-biofortified egg yolk, produced in hens fed biofortified orange maize or tangerine-fortified maize feeds, was freeze-dried and fed to gerbils...
August 1, 2018: Journal of Nutrition
Mei Li, Xiang Li, Jing Li, Mei Lu, Xuebo Liu, Xiang Duan
Multiple freeze-thaw (F-T) treatments could modify a protein structure and affect its physicochemical and biological activities. In this work, egg phosvitin (PSV) was subjected to multiple F-T treatments, and the changes in physicochemical and functional properties were investigated. The F-T treatments modified the molecular characteristics of PSV involving a decrease in surface hydrophobicity. Differential scanning calorimetry and scanning electron microscopy showed that PSV underwent denaturation, dissociation and possibly aggregation...
September 19, 2018: Food & Function
Marcia C Inhorn, Daphna Birenbaum-Carmeli, Lynn M Westphal, Joseph Doyle, Norbert Gleicher, Dror Meirow, Martha Dirnfeld, Daniel Seidman, Arik Kahane, Pasquale Patrizio
PURPOSE: What are the specific pathways that lead women to freeze their eggs? In this binational study, women were asked directly about the life circumstances that led them on the path to elective egg freezing (EEF). METHODS: From June 2014 to August 2016, 150 women (114 in the USA, 36 in Israel) who had completed at least 1 cycle of EEF were interviewed by two medical anthropologists. Study participants were recruited through four American IVF clinics (two academic, two private) and three in Israel (one academic, two private)...
August 3, 2018: Journal of Assisted Reproduction and Genetics
M C Inhorn, D Birenbaum-Carmeli, J Birger, L M Westphal, J Doyle, N Gleicher, D Meirow, M Dirnfeld, D Seidman, A Kahane, P Patrizio
BACKGROUND: What are the underlying socio-demographic factors that lead healthy women to preserve their fertility through elective egg freezing (EEF)? Many recent reviews suggest that women are intentionally postponing fertility through EEF to pursue careers and achieve reproductive autonomy. However, emerging empirical evidence suggests that women may be resorting to EEF for other reasons, primarily the lack of a partner with whom to pursue childbearing. The aim of this study is thus to understand what socio-demographic factors may underlie women's use of EEF...
July 23, 2018: Reproductive Biology and Endocrinology: RB&E
Marcia C Inhorn, Daphna Birenbaum-Carmeli, Lynn M Westphal, Joseph Doyle, Norbert Gleicher, Dror Meirow, Hila Raanani, Martha Dirnfeld, Pasquale Patrizio
Medical egg freezing (MEF) is being recommended increasingly for women at risk of losing their reproductive ability due to cancer chemotherapy or other fertility-threatening medical conditions. This first, binational, ethnographic study of women who had undergone MEF sought to explore women's experiences under two different funding systems: (i) the USA, where the cost of MEF is rarely covered by private or state health insurance; and (ii) Israel, where the cost of MEF is covered by national health insurance...
April 2018: Reproductive Biomedicine & Society Online
S Akhter, B A Rakha, M S Ansari, S Iqbal, M Khalid
Egg yolk is used as a cryoprotectant in semen preservation. However, its composition varies according to the species which may influence its effectiveness during the freeze-thaw process. Therefore, study was conducted to identify the optimum level of pigeon egg yolk (PEY) in Tris citric acid (TCA) extender for freezability and in vivo fertility of buffalo semen. Semen was collected at weekly intervals for a period of three weeks (replicates) from 6 Nili Ravi buffalo bulls (2 ejaculates/bull/replicate) and diluted with TCA extender (50 × 106 motile spermatozoa ml-1 ) containing 5%, 10%, 15% and 20% PEY or 20% CEY (control) and cryopreserved...
October 1, 2018: Theriogenology
Tran Hong Quan, Soottawat Benjakul
The effects of desugarization using glucose oxidase/catalase and spray-drying conditions on gelling properties of duck albumen powder were studied. Gelling temperatures increased as spray-drying inlet temperatures (140-180C) were increased (p < .05). ΔE*, a*-, and b*- values of gel increased but L* and whiteness decreased when higher spray-drying temperatures were used (p < .05). However, whiteness and lightness of albumen gel were drastically increased after desugarization (p < .05). Texture profile analysis showed that hardness, springiness, gumminess, and chewiness of gel decreased with increasing spray-drying temperatures...
October 2018: Journal of Texture Studies
Katie E Marshall, Brent J Sinclair
Internal ice formation leads to wholesale changes in ionic, osmotic and pH homeostasis, energy metabolism, and mechanical damage, across a small range of temperatures, and is thus an abiotic stressor that acts at a distinct, physiologically relevant, threshold. Insects that experience repeated freeze-thaw cycles over winter will cross this stressor threshold many times over their lifespan. Here, we examined the effect of repeatedly crossing the freezing threshold on short-term physiological parameters (metabolic reserves and cryoprotectant concentration) as well as long-term fitness-related performance (survival and egg production) in the freeze-tolerant goldenrod gall fly, Eurosta solidaginis We exposed overwintering prepupae to a series of low temperatures (-10, -15 or -20°C) with increasing numbers of freezing events (3, 6 or 10) with differing recovery periods between events (1, 5 or 10 days)...
August 13, 2018: Journal of Experimental Biology
Karim Benhenia, Hamza Rahab, Mustapha-Adnane Smadi, Hamza Benmakhlouf, Ali Lamara, Takfarinas Idres, Mokrane Iguer-Ouada
Vitamin E is a potent molecule, especially when loaded in cyclodextrin, in modulating oxidative stress during the freeze-thawing process. The present study aimed to investigate the effect of different concentrations of cyclodextrin-vitamin E complex (CD-Vit E) on cryopreserved ram sperm. Ejaculates collected from five adult rams were pooled and divided into four aliquots. All aliquots were treated in Tris-extender (Tris-glucose-citric acid) containing 2 mg cholesterol-loaded methyl-β- cyclodextrin/120 × 106 spermatozoa and either 0 (Control), 2, 4 or 6 mg CD-Vit E/120 × 106 spermatozoa, corresponding to 0, 0...
June 6, 2018: Animal Reproduction Science
Abouzar Najafi, Ramezan Ali Taheri, Mahdieh Mehdipour, Gholamreza Farnoosh, Felipe Martínez-Pastor
Antioxidants may ameliorate the effects of the freeze-thawing stress on cryopreserved spermatozoa. Lycopene is an effective antioxidant yet to be tested for rooster sperm cryopreservation and nanoliposomes, a technology recently applied to sperm cryopreservation, could improve conservation and antioxidant delivery to spermatozoa. Therefore, we evaluated the effect of 0.1, 0.2, and 0.3 mM lycopene and lycopene-loaded nanoliposomes (LnL) on the cryopreservation of rooster sperm in Beltsville extender. Post-thawing evaluation included sperm motility, membrane integrity, abnormal morphology, mitochondria activity, apoptotic status, malondialdehyde (MDA) and antioxidant activities: Glutathione peroxidase (GPx), superoxide dismutase (SOD) and total antioxidant capacity (TAC)...
May 25, 2018: Animal Reproduction Science
Imogen Goold
In 'Egg Freezing and Egg Banking: Empowerment and Alienation in Assisted Reproduction', John A Robertson responds to the American Society of Reproductive Medicine's statement that oocyte preservation should no longer be considered an experimental treatment. He explores the implications of this development, focusing on the potentially empowering impact of oocyte preservation as a means for women to preserve their fertility. He also engages with concerns about the possibility that such a development may raise issues of alienation...
December 2017: Journal of Law and the Biosciences
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