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Egg freezing

Niloofar Ziarati, Tohid Rezaei Topraggaleh, Pegah Rahimizadeh, Leila Montazeri, Saman Maroufizadeh, Mohammad Ali Sadighi Gilani, Abdolhossein Shahverdi
Application of an appropriate freezing carrier is crucial for improving post-thaw recovery of oligozoospermic samples. In this study, our purpose is developing a user-friendly, easy handling and close micro-quantity (MQ) straw along with different freezing media, for cryopreservation of oligozoospermic samples. Twenty oligozoospermic semen samples were collected and mixed with glycerol egg yolk citrate (GEYC) or Spermfreeze® (SPF) medium. The mixture was loaded into MQ straws, sealed and stored in liquid nitrogen (LN) vapor...
December 8, 2018: Cryobiology
Vinay Gunnala, Jessica Fields, Mohamad Irani, Debra D'Angelo, Kangpu Xu, Glenn Schattman, Zev Rosenwaks
OBJECTIVE: To investigate whether BRCA carriers with and without malignancy have decreased ovarian reserve at baseline compared with BRCA noncarriers. DESIGN: Retrospective cohort study. SETTING: Academic medical center. PATIENT(S): Seven-hundred and ninety-five oocyte cryopreservation patients, comprising BRCA carriers with and without malignancy (n = 57) and BRCA noncarriers (n = 738). INTERVENTION(S): Fertility preservation with oocyte cryopreservation...
December 5, 2018: Fertility and Sterility
Chunrong Lv, Guoquan Wu, Qionghua Hong, Guobo Quan
The cryotolerance of farm animal spermatozoa varies according to their specific features, such as size, shape, and lipid composition. Thus, it is impossible to develop a standardized freezing procedure for different kinds of livestock species. The establishment of an efficient semen cryopreservation procedure will facilitate long-term conservation of small ruminant genetic resources and extension of artificial insemination in daily production. Different from sheep, goat seminal plasma contains a phospholipase, which can affect spermatozoa viability through interaction with milk or egg yolk...
November 30, 2018: Biopreservation and Biobanking
Francesca Filippi, Fabio Martinelli, Alessio Paffoni, Marco Reschini, Francesco Raspagliesi, Edgardo Somigliana
PURPOSE: To evaluate the capacity of random antral follicle count (AFC), i.e., AFC recorded at any time during the menstrual cycle, to predict the number of retrieved mature oocytes in women with malignancies undergoing random start ovarian hyperstimulation METHODS: A consecutive series of 72 women with malignancies who underwent ovarian hyperstimulation aimed at egg freezing between July 2014 and December 2016 was retrospectively reviewed. A standardized random start protocol was used for all women...
November 26, 2018: Journal of Assisted Reproduction and Genetics
Anna Katharina Wöll, Juliane Schütz, Jana Zabel, Jürgen Hubbuch
Knowledge of protein behavior/stability during freeze/thaw (FT) operations is essential for storage and production processes in the biopharmaceutical industry. FT stress involves freeze concentration, cold denaturation, and ice crystals formation which can result in protein aggregation. Therefore, it is important to understand the ongoing FT processes, and the influence of different solution parameters. In order to evaluate the ongoing processes during FT (up to -80°C), phase diagrams with lysozyme from chicken egg white and sodium chloride were generated...
November 17, 2018: International Journal of Pharmaceutics
L Fraser, Ł Zasiadczyk, J Strzeżek, R Strzeżek, K Karpiesiuk
Lipoproteins, isolated from ostrich egg yolk (LPFo), provide excellent protection for boar spermatozoa against cryo-induced damage. The present study was performed to investigate the effects of LPFo on the freezability and fertilizing capacity of frozen-thawed (FT) boar semen after post-cervical artificial inseminations (post-CAIs). Semen, collected from 7 Polish Large White (PLW) and 4 Polish Landrace (PLR), was frozen in an extender containing LPFo. Post-CAIs were performed in 38 multiparous sows, using a catheter-cannula kit...
June 2018: Polish Journal of Veterinary Sciences
Felipe Martínez-Pastor, Mercedes Álvarez, Camino Guerra, César A Chamorro, Luis Anel-López, Paulino de Paz, Luis Anel, Manuel Álvarez-Rodríguez
Germplasm banking is a key technology enabling the ex-situ conservation of wild species. However, cryopreservation protocols must be tested to assure the applicability of the banked material. The objective of this study was defining a range of parameters for the composition of a semen extender for Cantabrian chamois epididymal spermatozoa (post-mortem collection). The freezing extender was based in a TES-Tris-fructose buffer, modifying its composition in three experiments: Osmolality of the buffer (320, 380 or 430 mOsm/kg, 8% glycerol, 15% egg yolk), glycerol (4 or 8%, 430 mOsm/kg, 15% egg yolk), egg yolk (10 or 15%, 430 mOsm/kg, 4% glycerol)...
October 29, 2018: Theriogenology
Zion Ben-Rafael
Delayed childbearing in affluent countries and the financial crisis of the Y-generation have contributed to the dramatic decline in birth rate. Social oocyte freezing (SOF) has fuelled the imagination of patients and doctors to offer it as a solution to single, presumably fertile, women to preserve their fertility potential by egg banking at an early age. Some are calling on governments to support large-scale 'fertility preservation', but is it cost-effective? Social oocyte freezing is effectively expensive insurance, where future utilization is unknown...
October 2018: Reproductive Biomedicine Online
Mary Hagedorn, Zoltan Varga, Ronald B Walter, Terrence R Tiersch
The genetic resources of aquatic biomedical model organisms are the products of millions of years of evolution, decades of scientific development, and hundreds of millions of dollars of research funding investment. Genetic resources (e.g., specific alleles, transgenes, or combinations) of each model organism can be considered a form of scientific wealth that can be accumulated and exchanged, typically in the form of live animals or germplasm. Large-scale maintenance of live aquatic organisms that carry these genetic resources is inefficient, costly, and risky...
October 31, 2018: Cryobiology
Kylie Baldwin, Lorraine Culley
Very few studies have explored women's experiences of social egg freezing and the limited primary research on this topic has suggested that users find the process of freezing eggs emotionally challenging. We, therefore, undertook semi-structured interviews with 31 women who identified as undergoing egg freezing for social reasons in order to explore how female users of social egg freezing technology reported their experience of freezing eggs for 'social' reasons. Interviews lasted between 40 minutes and 2 hours, were audio recorded and transcribed verbatim...
November 1, 2018: Human Fertility: Journal of the British Fertility Society
Hugh A Smith, Curtis A Nagle, Charles M MacVean, Gary E Vallad, Edzard van Santen, Samuel F Hutton
Bemisia tabaci MEAM1 (Gennadius), the sweetpotato whitefly, transmits Tomato yellow leaf curl virus (TYLCV), which causes significant yield losses annually in Florida and other tomato-producing regions. Field trials were carried out at the University of Florida's Gulf Coast Research and Education Center to evaluate integration of plastic mulch type, at-plant insecticide, and tomato variety for management of the sweetpotato whitefly and TYLCV. The tomato varieties Charger, Rally, and Tygress had significantly lower season-long densities of whitefly eggs and nymphs than Florida-47 in one or more trials...
October 30, 2018: Journal of Economic Entomology
Paloma Rocha Arakaki, Marcílio Nichi, Frederico Ozanan Barros Monteiro, José Augusto Pereira Carneiro Muniz, Marcelo Alcindo de Barros Vaz Guimarães, Rodrigo Del Rio do Valle
BACKGROUND: The common marmoset (Callithrix jacchus) is one of the most studied Neotropical primates regarding reproduction. However, little has been reported on the black-tufted-ear marmoset (Callithrix penicillata), which may produce fertile hybrids with other Callithrix. This is the first study to evaluate sperm freezing from black-tufted-ear marmoset. METHODS: Testicles from all animals were measured, and semen was collected by penile vibrostimulation. Samples were analyzed after collection, after chilling and addition of glycerol, and after thawing...
October 21, 2018: Journal of Medical Primatology
Avinesh R Byreddy, Prusothman Yoganantharjah, Adarsha Gupta, Yann Gibert, Munish Puri
A 28-day feeding experiment with formulated feed using docosahexaenoic acid (DHA)-rich whole cells of freeze-dried marine microalgae Schizochytrium sp. to understand the distribution of fatty acids in a laboratory model zebrafish was conducted. Three feeds, commercial feed, 50:50 feed (50% commercial and 50% algae), and pure algae, were investigated. All feeds were consumed by zebrafish and showed optimal growth and weight gain with a survival rate of 100%. Lipids were extracted from four different tissues, brain, liver, muscle, and blood, to understand the distribution of fatty acids with respect to the feed...
October 22, 2018: Applied Biochemistry and Biotechnology
Lucie Gavin-Plagne, Loris Commin, Pierre Bruyère, Samuel Buff, Thierry Joly
Animal-derived products are widely used in sperm cryopreservation for their cryoprotective properties. These components, however, tend to be replaced because of sanitary risks. STEMALPHA.CRYO3 (Ref. 5617; Stem Alpha, Saint-Genis-l'Argentière, France), called "CRYO3," is a chemically defined preservation medium currently used for freezing human tissue and adult stem cells. The aim of this study was to evaluate the effect of a CRYO3-based medium on ram sperm freezing regarding in vitro parameters and in vivo fertility...
October 16, 2018: Biopreservation and Biobanking
Manuel T Orrego, Sofía I Melian, Judith Montenegro, Alejandra N Cimato, Humberto Cisale, Lidia L Piehl
Increased protein tyrosine phosphorylation and the appearance of a phosphorylated protein of 32 kD (p32) are reported among the capacitation-like changes in cryopreserved boar sperm. Egg yolk freezing extenders are composed by two fractions: insoluble granules and soluble plasma, which contains the low density lipoproteins (LDL) proposed as responsible for the egg yolk cryoprotective action. The aim of this work was to analyze the effects of complete egg yolk and its insoluble, soluble and LDL fractions on boar sperm quality and protein tyrosine phosphorylation after the first stage of a standard cryopreservation protocol...
January 1, 2019: Theriogenology
M Álvarez-Rodriguez, M Álvarez, L Anel-López, C Guerra, C A Chamorro, L Anel, P de Paz, F Martínez-Pastor
Genome Resource Banks are keystones in the ex-situ conservation of wild species. Post-mortem (PM) collection of epididymal spermatozoa is an opportunistic and valuable source of germplasm, the time from the death of the animal limits its use. Seeking to improve germplasm preservation strategies for the chamois (Rupicapra sp.), the effect of PM time on epididymal sperm quality and freezability was studied using the Cantabrian chamois. Samples were classified according to PM collection time, up to 216 h (refrigerated), and cryopreserved (Tris-citric acid-fructose, 430 mOsm/kg, 15% egg yolk, 8% glycerol; freezing at -20 °C/min)...
November 2018: Animal Reproduction Science
Luana Grasiele Pereira Bezerra, Ana Liza Paz Souza, Herlon Victor Rodrigues Silva, Fábio Roger Vasconcelos, Arlindo de Alencar Araripe Moura, Alexsandra Fernandes Pereira, Moacir Franco de Oliveira, Alexandre Rodrigues Silva
The aim was to describe, through scanning electron microscopy (SEM) and transmission electron microscopy (TEM), the ultrastructure of peccaries' fresh and frozen-thawed sperm. For that, semen derived from three mature males was obtained by electroejaculation and evaluated for motility, membrane integrity, membrane functionality, chromatin integrity, and morphology through light microscopy. Samples were frozen using a Tris extender plus egg yolk (20%) and glycerol (6%). Then, fresh and frozen-thawed semen samples were mixed in different sperm pools that were processed for SEM and TEM...
October 8, 2018: Microscopy Research and Technique
Guiulfo Mamani-Mango, Milagros Moina Gonzales, Martín Ramos Hidalgo, José Mendoza Mallma, Jaime Ruiz Béjar, Victoria Rivas Palma, Edwin Mellisho Salas
In alpacas, improvement of reproductive efficiency of male camelids is limited by the small testicular size, low spermatozoa production, and low quality of semen. In this study we aim to evaluate the effect of two extenders and two freezing rates on post-thaw quality of sperm recovered from alpaca epididymis with two methods (flushing and mincing), and to evaluate the in vitro fertilization (IVF) capacity of frozen sperm selected with two different selection methods (washing and swim-up). Sperm samples were processed with Tris-egg yolk or Bioxcell® extenders and frozen with slow freezing and fast freezing...
September 26, 2018: Biopreservation and Biobanking
Lisa Campo-Engelstein, Rohia Aziz, Shilpa Darivemula, Jennifer Raffaele, Rajani Bhatia, Wendy M Parker
In 2012, the American Society for Reproductive Medicine (ASRM) lifted the experimental label on oocyte preservation, but cautioned against women using it to avoid age-related infertility, known as social egg freezing (SEF). In 2014, Facebook and Apple announced that they would offer SEF as a workplace benefit. Within the context of a rapidly growing market for SEF, we were interested in how these two decisions affected media discussions, given that such discourse can strongly influence public perceptions and behaviors...
September 20, 2018: AJOB Empirical Bioethics
Benjamin P Jones, Paul Serhal, Jara Ben Nagi
No abstract text is available yet for this article.
September 19, 2018: Acta Obstetricia et Gynecologica Scandinavica
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