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Iron AND Transferrin

Lorenzo Calisti, Matilde Cardoso Trabuco, Alberto Boffi, Claudia Testi, Linda Celeste Montemiglio, Amédée des Georges, Irene Benni, Andrea Ilari, Bartłomiej Taciak, Maciej Białasek, Tomasz Rygiel, Magdalena Król, Paola Baiocco, Alessandra Bonamore
Ferritin H-homopolymers have been extensively used as nanocarriers for diverse applications in the targeted delivery of drugs and imaging agents, due to their unique ability to bind the transferrin receptor (CD71), highly overexpressed in most tumor cells. In order to incorporate novel fluorescence imaging properties, we have fused a lanthanide binding tag (LBT) to the C-terminal end of mouse H-chain ferritin, HFt. The HFt-LBT possesses one high affinity Terbium binding site per each of the 24 subunits provided by six coordinating aminoacid side chains and a tryptophan residue in its close proximity and is thus endowed with strong FRET sensitization properties...
2018: PloS One
Aseem K Tiwari, Gunjan Bhardwaj, Dinesh Arora, Geet Aggarwal, Swati Pabbi, Ravi C Dara, Ritesh Sachdev, Arun Raizada, Monisha Sethi
BACKGROUND: It is important to detect Latent Iron Deficiency (LID) to prevent development of an overt iron deficiency anemia. Early detection is difficult by using conventional hematological and biochemical parameters. Soluble transferrin receptor (sTfR) is presently the gold standard for diagnosing LID. We evaluated the utility of Reticulocyte Hemoglobin Equivalent (Ret-He), a newer hematological parameter, to predict LID in blood donors as compared to sTfR. METHODS: This was a randomized prospective study performed on 501 donor samples over a period of three-months...
August 12, 2018: Vox Sanguinis
Donald A McClain, Neeraj K Sharma, Shalini Jain, Alexandria Harrison, Lipika N Salaye, Mary E Comeau, Carl D Langefeld, Felipe R Lorenzo, Swapan K Das
Context: Excessive body iron stores are a risk factor for decreased insulin sensitivity (SI) and diabetes. We hypothesize that transcriptional dysregulation of genes involved in iron metabolism in adipocytes cause insulin resistance. Objective and Design: To define the genetic regulation of iron metabolism and its role in SI, we used gene expression, genotype, and insulin sensitivity data from an African American cohort (N=256). Replication studies were performed in independent European ancestry cohorts...
August 6, 2018: Journal of Clinical Endocrinology and Metabolism
Toshihide Hayashi, Yuri Tanaka, Masaki Iwasaki, Hiroki Hase, Hiroyuki Yamamoto, Yasuhiro Komatsu, Ryoichi Ando, Masato Ikeda, Daijo Inaguma, Toshifumi Sakaguchi, Toshio Shinoda, Fumihiko Koiwa, Shigeo Negi, Toshihiko Yamaka, Takashi Shigematsu, Nobuhiko Joki
OBJECTIVES: High prevalence of iron deficiency (ID) and cardiomyopathy have been observed in patients with end-stage kidney disease (ESKD). Our objective was to clarify associations between ID and cardiac remodeling in patients with ESKD. DESIGN AND METHODS: A cross-sectional study was conducted using 1974 Japanese patients with ESKD at the initiation of maintenance dialysis. Levels of hemoglobin (Hb), iron status, and cardiac enlargement as assessed by the cardiothoracic ratio (CTR) were determined immediately before the first hemodialysis session...
August 7, 2018: Journal of Renal Nutrition
Bruna Taciane da Silva Bortoleti, Manoela Daiele Gonçalves, Fernanda Tomiotto-Pellissier, Milena Menegazzo Miranda-Sapla, João Paulo Assolini, Amanda Cristina Machado Carloto, Priscila Goes Camargo de Carvalho, Ian Lucas Alves Cardoso, Andréa Name Colado Simão, Nilton Syogo Arakawa, Idessania Nazareth Costa, Ivete Conchon-Costa, Wander Rogério Pavanelli
BACKGROUND: American tegumentary leishmaniasis (ATL) is a zoonotic disease caused by protozoa of the genus Leishmania. The high toxicity, high costs and resistance of some strains to current drugs has prompted the search for therapeutic alternatives for the management of this disease. Sphagneticola trilobata is a plant that has diterpenes as main constituents, including grandiflorenic acid (GFA) that has antiinflammatory, antiprotozoal, antibacterial and antinociceptive activity. PURPOSE: The aim of our study was to determine the effect of GFA on both the promastigotes and the amastigotes of Leishmania amazonensis...
July 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Julia Ruhe, Christoph Waldeyer, Francisco Ojeda, Alev Altay, Renate B Schnabel, Sarina Schäfer, Karl J Lackner, Stefan Blankenberg, Tanja Zeller, Mahir Karakas
Intrinsic iron release is discussed to have favorable effects in coronary artery disease (CAD). The aim of this study was to evaluate the prognostic relevance of intrinsic iron release in patients with CAD. Intrinsic iron release was based on a definition including hepcidin and soluble transferrin receptor (sTfR). In a cohort of 811 patients with angiographically documented CAD levels of hepcidin and sTfR were measured at baseline. Systemic body iron release was defined as low levels of hepcidin (<24 ng/mL) and high levels of sTfR (≥2 mg/L)...
August 9, 2018: Biomolecules
Sweta Mukherjee, Mukti Sharma, Amit Devgan, S K Jatana
Background: Iron deficiency anemia (IDA) in cyanotic congenital heart disease (CCHD) and its association with cyanotic spells has been documented in literature. However, Indian data especially in the pediatric age group is scarce. This study was conducted to find out the prevalence of IDA in this population. Methods: An observational study was conducted in a tertiary care hospital. Children with CCHD in the age group of birth-12 years were included in the study...
July 2018: Medical Journal, Armed Forces India
Ashlee J Conway, Fiona C Brown, Gerhard Rank, Benjamin T Kile, Craig J Morton, Stephen M Jane, David J Curtis
To identify novel regulators of erythropoiesis, we performed independent forward genetic screens using the chemical mutagen ENU in mice. Among progeny displaying microcytic red-cell phenotypes, 7 independent mouse strains harboring mutations within the transferrin receptor gene Tfrc were identified. Six of the mutants, including the previously described red blood cell 6 (RBC6) strain, displayed reduced erythroblast CD71 expression and midgestation lethality of homozygotes (E12.5-E14.5), and 1 novel strain, RBC21, displayed a variable phenotype with sustained CD71 expression and late homozygous lethality (E18...
August 14, 2018: Blood Advances
Mariadelina Simeoni, Maria Lucia Citraro, Francesca Deodato, Michele Provenzano, Maria Capria, Alessandro Comi, Emanuela Libri, Michele Andreucci, Arturo Puja, Ludovico Abenavoli, Massimo Cocchi, Giorgio Fuiano
PURPOSE: Gut dysbiosis has been described in advanced, but not in initial stages of CKD. Considering the relevant impact of gut dysbiosis on renal and cardiovascular risk, its diagnosis and treatment are clinically relevant. METHODS: We designed, open-label, placebo-controlled intervention study (ProbiotiCKD) to evaluate gut microbiota metabolism in a cohort of KDIGO CKD patients (n = 28) at baseline and after a randomly assigned treatment with probiotics or placebo...
August 3, 2018: European Journal of Nutrition
Dengpiao Xie, Jianting Wang, Xinping Wu, Mingquan Li
BACKGROUND: The efficacy of daprodustat for the treatment of anemic patients with chronic kidney disease (CKD) remains controversial. The aim of the study is to perform a meta-analysis of randomized controlled trials to evaluate the efficacy and safety of daprodustat for anemic patients with chronic kidney disease. METHODS: We searched Medline, Embase, Science Citation Index, Cochrane Central Register of Controlled Trials, and Clinical Trial Registries for randomized controlled trials comparing daprodustat with placebo for anemic patients with CKD...
August 2, 2018: International Urology and Nephrology
Aleksandra Śliwińska, Justyna Luty, Ewa Aleksandrowicz-Wrona, Sylwia Małgorzewicz
BACKGROUND: Iron is one of the nutrients that require special consideration in a plant-based diet. The widespread belief is that meat is the best source of iron and a vegetarian diet increases the risk of its deficiency. This conviction has been the subject of analysis in a growing number of scientific reports. OBJECTIVES: The aim of this study was to assess the iron intake and iron metabolism in vegetarians and vegans compared to a control group. MATERIAL AND METHODS: A total of 55 vegetarians and 36 healthy volunteers were studied...
July 31, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
Natasja Lammers, Ashton Curry-Hyde, Anne J Smith, Peter R Eastwood, Leon M Straker, David Champion, Nigel McArdle
Restless legs syndrome has been associated with serum iron deficiency in clinical studies. However, studies investigating this relationship have had inconsistent results and there are no studies in young adults. Therefore, we investigated the relationship between serum measures of iron stores and restless legs syndrome in young adults in the community. Participants in the Western Australian Pregnancy Cohort (Raine) Study answered questions on restless legs syndrome (n = 1,100, 54% female) at age 22 years, and provided serum measures of iron stores (ferritin and transferrin saturation) at ages 17 and 22 years...
July 31, 2018: Journal of Sleep Research
Angela C Flynn, Shahina Begum, Sara L White, Kathryn Dalrymple, Carolyn Gill, Nisreen A Alwan, Mairead Kiely, Gladys Latunde-Dada, Ruth Bell, Annette L Briley, Scott M Nelson, Eugene Oteng-Ntim, Jane Sandall, Thomas A Sanders, Melissa Whitworth, Deirdre M Murray, Louise C Kenny, Lucilla Poston, On Behalf Of The On Behalf Of The Scope And Upbeat Consortiums
: Obesity in pregnancy may negatively influence maternal and infant iron status. The aim of this study was to examine the association of obesity with inflammatory and iron status in both mother and infant in two prospective studies in pregnancy: UPBEAT and SCOPE. Maternal blood samples from obese ( n = 245, BMI ≥ 30 kg/m²) and normal weight ( n = 245, BMI < 25 kg/m²) age matched pregnant women collected at approximately 15 weeks' gestation, and umbilical cord blood samples collected at delivery, were analysed for a range of inflammatory and iron status biomarkers...
July 30, 2018: Nutrients
En Xu, Min Chen, Jiashuo Zheng, Zaitunamu Maimaitiming, Tianjiao Zhong, Huijun Chen
Multi-copper ferroxidases (MCFs) play important roles in cellular iron metabolism and homeostasis. In this study, we generated the hephaestin (Heph), ceruloplasmin (Cp) single and Heph/Cp double knockout (KO) mice to investigate the roles of MCFs in iron transport among system and vital organs in mice at 4 weeks and 6 months of age. Compared with wild-type (WT) mice, Heph/Cp mice at both ages presented with severe anemia and significantly lower iron level in the serum and spleen, but with significantly higher iron level in the liver, heart, kidney, and duodenal enterocytes...
July 27, 2018: Biochemical and Biophysical Research Communications
Timothy H Ciesielski, Joel Schwartz, David C Bellinger, Russ Hauser, Chitra Amarasiriwardena, David Sparrow, Robert O Wright
BACKGROUND: Because iron and cadmium share common transport mechanisms, iron-processing protein variants such as HFE C282Y, HFE H63D, and Transferrin P570S may influence cadmium metabolism. Our aim was to evaluate associations between common HFE and Transferrin polymorphisms and toenail cadmium levels among older men. METHODS: In a longitudinal cohort of men age 51-97, the Normative Aging Study (NAS), we evaluated toenail cadmium concentrations and missense single nucleotide polymorphisms (SNPs) in the HFE and Transferrin genes...
July 26, 2018: Environment International
Monika Kałużna-Czyż, Urszula Grzybowska-Chlebowczyk, Halina Woś, Sabina Więcek
Introduction: Iron deficiency is common in patients with cystic fibrosis. Conventional iron status markers are often abnormal in patients with CF, reflecting inflammation and/or infection, rather than actual iron stores. The aim was to evaluate serum hepcidin levels against selected iron status markers, assuming that hepcidin may be a more sensitive indicator of iron management in patients with active inflammation, such as those with CF. Material and Methods: 46 children with cystic fibrosis and 31 healthy controls were enrolled...
2018: Mediators of Inflammation
Gary R Lichtenstein, Jane E Onken
AIMS: To compare the efficacy and safety of intravenous (IV) ferric carboxymaltose (FCM) versus oral iron and other IV iron therapies in patients with iron-deficiency anemia (IDA) resulting from gastrointestinal (GI) disorders. METHODS: A pooled analysis of four prospective, randomized, active-controlled trials in patients with IDA was performed. Efficacy measures included change from baseline in hemoglobin (Hb), ferritin, and transferrin saturation (TSAT) and correlations of baseline Hb, ferritin, and TSAT to change in Hb...
July 28, 2018: Digestive Diseases and Sciences
Izumi Yanatori, Fumio Kishi
Many past and recent advances in the field of iron metabolism have relied upon the discovery of divalent metal transporter 1, DMT1 in 1997. DMT1 is the major iron transporter and contributes non-heme iron uptake in most types of cell. Each DMT1 isoform exhibits different expression patterns in cell-type specificity and distinct subcellular distribution, which enables cells to uptake both transferrin-bound and non-transferrin-bound irons efficiently. DMT1 expression is regulated by iron through the translational and degradation pathways to ensure iron homeostasis...
July 25, 2018: Free Radical Biology & Medicine
Fleur Wolff, Sandra De Breucker, Thierry Pepersack, Nathalie Compté, Christian Mélot, Béatrice Gulbis, Frédéric Cotton
Background Anaemia is often multifactorial in the elderly, with a frequent association between iron deficiency anaemia (IDA) and anaemia of chronic disease (ACD). The primary objective of our study was to investigate whether baseline hepcidin measurement could be useful for identifying iron deficiency (ID) in anaemic elderly patients. The secondary objective was to assess whether baseline hepcidin concentrations correlated with the relative increase of transferrin saturation (TS) after an oral iron absorption test (OIAT)...
July 28, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
Hyang Ki Min, Su Ah Sung, Yun Kyu Oh, Yeong Hoon Kim, Wookyung Chung, Sue K Park, Curie Ahn, Sung Woo Lee
Background: Few studies have examined the association between hepcidin, iron indices and bone mineral metabolism in non-dialysis chronic kidney disease (CKD) patients. Methods: We reviewed the data of 2238 patients from a large-scale multicenter prospective Korean study (2011-16) and excluded 214 patients with missing data on markers and related medications of iron and bone mineral metabolism, hemoglobin, blood pressure and causes of CKD. Multivariate linear regression analysis was used to identify the association between iron and bone mineral metabolism...
July 23, 2018: Nephrology, Dialysis, Transplantation
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