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Conversion gastric cancer

Li Zhang, Qinghua Wu, Changyu He, Dongyu Liang, Qingqing Yi, Junfeng Shi, Boshun Wan, Rong Yang, Luyi Li, Shuang Sha, Qing Chang
BACKGROUND: HOXB9 is a homeobox transcription factor which plays an important role in carcinoma development. This protein has been shown to inhibit cancer cell proliferation. However, the mechanisms that underpin HOXB9-mediated inhibition of cellular proliferation remain to be elucidated. METHODS: In this study, two gastric cancer cell lines, SGC7901 and MKN45, were transfected with plasmids pLVX-HOXB9 and shHOXB9. These transfections resulted in the over-expression of the HOXB9 gene in the SGC7901/HOXB9 cells and knockdown of the HOXB9 gene in the MKN45/shHOXB9 cells...
August 24, 2018: Digestive and Liver Disease
Yongfan Xin, Shili Ning, Liang Zhang, Ming Cui
BACKGROUND/AIMS: Lymph node metastasis is the primary cause of cancer-related death among patients with gastric cancer (GC), and cell division cycle 27 (CDC27) promotes the metastasis and epithelial-mesenchymal transition in many cancers. Till now, the mechanisms underlying CDC27-induced the epithelial-mesenchymal transition (EMT) of GC are still unclear. METHODS: We analyzed the expression levels of CDC27 and EMT-related biomarkers using immunohistochemistry and Western blot in 60 cases of GC tissues, and then GC cells with CDC27 shRNAs or plasmids were subjected to in vitro and in vivo assays, including CCK-8, wound healing and transwell assays...
October 11, 2018: Cellular Physiology and Biochemistry
Q Feng, J R Zhao, A X Zhang, S L Li
Objective: To investigate the efficacy and safety of lobaplatin (LBP) plus S-1 for advanced gastric cancer (AGC) and determine the potential role of circulating tumor cells (CTC) for predicting the therapeutic response and prognosis. Methods: From January 2014 to February 2015, 64 consecutive patients with AGC received lobaplatin plus S-1 chemotherapy in Liaocheng People's Hospital. The clinical features, clinical response, adverse effects, prognosis and CTC pre- and post-treatment were retrospectively analyzed...
September 23, 2018: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
Gan Cao, Dehu Chen, Guiyuan Liu, Yan Pan, Qinghong Liu
Background: Cytoplasmic polyadenylation element-binding protein 4 (CPEB4) has previously been reported to be associated with biological malignancy in various cancers. However, its function in tumor growth and metastasis in gastric cancer (GC) remains obscure. Here, we explored the functional and molecular mechanisms by which CPEB4 influences GC. Materials and methods: The expression of CPEB4 was assessed using Western blot and immunohistochemistry in GC specimens...
2018: OncoTargets and Therapy
Samana Batool, Thomson Patrick Joseph, Mushraf Hussain, Miza S Vuai, Kavish H Khinsar, Syed Riaz Ud Din, Arshad Ahmed Padhiar, Mintao Zhong, Anhong Ning, Wei Zhang, Jing Cao, Min Huang
Present study aimed to elucidate the anticancer effect and the possible molecular mechanism underlying the action of Latcripin 1 (LP1), from the mushroom Lentinula edodes strain C91-3 against gastric cancer cell lines SGC-7901 and BGC-823. Cell viability was measured by Cell Counting Kit-8 (CCK-8); morphological changes were observed by phase contrast microscope; autophagy was determined by transmission electron microscope and fluorescence microscope. Apoptosis and cell cycle were assessed by flow cytometer; wound-healing, transwell migration and invasion assays were performed to investigate the effect of LP1 on gastric cancer cell's migration and invasion...
September 30, 2018: International Journal of Molecular Sciences
Samir Gupta, Li Tao, James D Murphy, M Constanza Camargo, Eyal Oren, Mark A Valasek, Scarlett Lin Gomez, Maria Elena Martinez
Anatomic-subsite risk factors for gastric cancer differ substantially, and subsite-specific distribution of risk factors (such as Helicobacter pylori) may vary by race/ethnicity and neighborhood socioeconomic status (nSES). We examined differences in gastric cancer incidence by subsite, stratified by race/ethnicity and nSES utilizing Surveillance Epidemiology and End Results Program 2000-2014 data for 77,881 incident gastric cancer cases (n=23,651 cardia, n=35,825 non-cardia; n=18,405 overlapping/unspecified)...
September 26, 2018: Gastroenterology
Masaki Nakamura, Toshiyasu Ojima, Mikihito Nakamori, Masahiro Katsuda, Toshiaki Tsuji, Keiji Hayata, Tomoya Kato, Hiroki Yamaue
BACKGROUND: Patients with positive peritoneal cytology (CY1) or peritoneal dissemination (P1) have significantly poor prognosis. We performed pre-therapeutic staging laparoscopy (SL) to diagnose peritoneal metastasis for patients with advanced gastric cancer. When peritoneal metastasis disappears by chemotherapy for patients with CY1 or P1, we have intention to perform conversion surgery (CS). This study aims to clarify the clinical significance of CS for such patients. METHODS: We retrospectively analyzed clinical outcomes of 115 patients with advanced gastric cancer (large type 3, type 4, serosa-invasion) who underwent SL between 2005 and 2014...
September 27, 2018: Journal of Gastrointestinal Surgery: Official Journal of the Society for Surgery of the Alimentary Tract
Yalan Lu, Deqiang Han, Wenjie Liu, Rong Huang, Jinhuan Ou, Xiaoqiao Chen, Xizhe Zhang, Xuezhi Wang, Shijun Li, Lin Wang, Changzheng Liu, Shiying Miao, Linfang Wang, Changwu Ma, Wei Song
Chemotherapy resistance represents a major issue associated with gastric cancer (GC) treatment, and arises through multiple mechanisms, including modulation of the cell-cycle check point. Several ubiquitin kinases, including RING finger protein 138 (RNF138), have been reported to mediate the G2/M phase arrest. In this study, we investigated the role of RNF138 in the development of cisplatin resistance of two GC cell lines. We show that RNF138 levels are higher in cisplatin-resistant cell lines, compared with cisplatin-sensitive cells, and RNF138 expression was elevated during drug withdrawal following the cisplatin treatment...
September 27, 2018: Cancer Biology & Therapy
Yasuhiro Kodera
Gastric cancer with metastases outside of the regional lymph nodes is deemed oncologically unresectable. Nevertheless, some metastatic lesions are technically resectable by applying established surgical techniques such as para-aortic lymphadenectomy and hepatectomy. At the time of compilation of the Japanese gastric cancer treatment guidelines version 4, systematic reviews were conducted to see whether it is feasible to make any recommendation to dissect both the primary and metastatic lesions with intent to cure, possibly as part of multimodality treatment...
September 2018: Annals of gastroenterological surgery
Toshiyuki Tanahashi, Kazuya Yamaguchi, Itaru Yasufuku, Yoshihiro Tanaka, Nobuhisa Matsuhashi, Takao Takahashi, Kazuhiro Yoshida
No abstract text is available yet for this article.
September 2018: Gan to Kagaku Ryoho. Cancer & Chemotherapy
Simonetta Buglioni, Elisa Melucci, Francesca Sperati, Matteo Pallocca, Irene Terrenato, Francesca De Nicola, Frauke Goeman, Beatrice Casini, Carla Azzurra Amoreo, Enzo Gallo, Maria Grazia Diodoro, Edoardo Pescarmona, Patrizia Vici, Domenico Sergi, Laura Pizzuti, Luigi Di Lauro, Marco Mazzotta, Maddalena Barba, Maurizio Fanciulli, Ilio Vitale, Ruggero De Maria, Gennaro Ciliberto, Marcello Maugeri-Saccà
Whether PD-L1 expression is associated with survival outcomes in gastric cancer (GC) is controversial. The inhibition of the PD-1/PD-L1 pathway is effective against genomically unstable tumors. Hypothesizing that also the clinical significance of PD-L1 might be dependent on the activation of molecular circuits ensuring genomic stability, we evaluated PD-L1 expression in tissue samples from 72 advanced GC patients treated with first-line chemotherapy. Samples were already characterized for DNA damage repair (DDR) component expression (pATM, pChk1, pWee1, γ-H2AX and pRPA2) along with mutations in DDR-linked genes ( TP53 and ARID1A )...
2018: Oncoimmunology
Sang-Woong Lee, Masaru Kawai, Keitaro Tashiro, Satoshi Kawashima, Ryo Tanaka, Keitaro Tanaka, Eiji Nomura, Kazuhisa Uchiyama
BACKGROUND: Totally laparoscopic gastrectomy (LG) is preferred over open gastrectomy because it allows safe anastomosis, a small wound, and early bowel recovery. However, esophagojejunostomy (EJS) following laparoscopic total gastrectomy (LTG) remains technically challenging. To popularize LTG, a secure method of reconstruction must be developed. We present a simple and safe technique for intracorporeal EJS following LTG. METHODS: Our modified technique for intracorporeal EJS as a part of Roux-en-Y reconstruction following LTG incorporates an isoperistaltic stapled EJS with closure of the entry hole using two unidirectional barbed sutures...
September 5, 2018: Surgical Endoscopy
Ulrich Ronellenfitsch, Kristina Ernst, Christina Mertens, Marcus J Trunk, Philipp Ströbel, Alexander Marx, Peter Kienle, Stefan Post, Kai Nowak
INTRODUCTION: Survival in gastric cancer is often limited by peritoneal carcinomatosis, which supposedly develops from serosal tumor infiltration or tumor cell spread during gastrectomy with lymphadenectomy. To eliminate peritoneal tumor cells, extensive intraperitoneal lavage (EIPL) has been suggested. Impressive results have been achieved in Japanese trials. In this trial, we assessed EIPL in Western patients. METHODS: This prospective trial included patients with non-metastatic gastric adenocarcinoma undergoing gastrectomy with D2 lymphadenectomy...
September 5, 2018: Tumori
Jingya Wang, Xuwen Guan, Yue Zhang, Shaohua Ge, Le Zhang, Hongli Li, Xia Wang, Rui Liu, Tao Ning, Ting Deng, Haiyang Zhang, Xiangli Jiang, Yi Ba, Dingzhi Huang
BACKGROUND/AIMS: The malignant biological behavior of gastric cancer(GC) is not only determined by cancer cells alone, but also closely regulated by the microenvironment. Fibroblasts represent a large proportion of the components in the tumor microenvironment, and they promote the development of disease. Currently, accumulating evidence suggests that exosomes can function as intercellular transport systems to relay their contents, especially microRNAs(miRNAs). METHODS: First, we detected the highly-expressed level of miR-27a in exosomes isolated from gastric cancer cells by qRT-PCR...
2018: Cellular Physiology and Biochemistry
Tae Woo Kim, Seon Young Lee, Mia Kim, Chunhoo Cheon, Seong-Gyu Ko
Kaempferol, a flavonoid, found in traditional medicine, fruits, and vegetables, and an HDAC inhibitor, is a powerful anti-cancer reagent against various cancer cell lines. However, detailed mechanisms involved in the treatment of gastric cancer (GC) using kaempferol are not fully understood. In our study, we investigated the biological activity and molecular mechanism involved in kaempferol-mediated treatment of GC. Kaempferol promoted autophagy and cell death, and increased LC3-I to LC3-II conversion and the downregulation of p62 in GC...
August 29, 2018: Cell Death & Disease
Jan-Willem Theunissen, Allen G Cai, Maryam M Bhatti, Anthony B Cooper, Andrew D Avery, Ryan Dorfman, Sebastian Guelman, Zoia Levashova, Thi-Sau Migone
The primary function of Tissue Factor (TF) resides in the vasculature as a cofactor of blood clotting; however, multiple solid tumors aberrantly express this transmembrane receptor on the cell surface. Here, we developed anti-TF antibody-drug conjugates (ADCs) that did not interfere with the coagulation cascade and benchmarked them against previously developed anti-TF ADCs. After screening an affinity-matured antibody panel of diverse paratopes and affinities, we identified one primary paratope family that did not inhibit conversion of Factor X (FX) to activated Factor X (FXa) and did not affect conversion of prothrombin to thrombin...
August 20, 2018: Molecular Cancer Therapeutics
Xiangqian Zhang, Min Zhang, Guifeng Wang, Ye Tian, Xiaolong He
It has previously been demonstrated that miRNA (miR)‑647 exhibits an important role in various cancers, and is aberrantly expressed in gastric cancer (GC). However, the exact role of miR‑647 in GC still remains unclear. The present study aimed to investigate the functional significance of miR‑647 and its target gene in GC. TargetScan and Miranda databases were used to predict the putative targets, and the prediction was validated by Dual‑luciferase Reporter Assays. To investigate whether miR‑647 affects GC cell behavior, a stable miR‑647‑overexpression/low‑expression cell line was generated by transfection with miR‑647 mimic/inhibitor...
August 6, 2018: Molecular Medicine Reports
Ho Jeong Lee, Venu Venkatarame Gowda Saralamma, Seong Min Kim, Sang Eun Ha, Suchismita Raha, Won Sup Lee, Eun Hee Kim, Sang Joon Lee, Jeong Doo Heo, Gon Sup Kim
Pectolinarigenin (PEC), a natural flavonoid present in Cirsium chanroenicum and in some species of Citrus fruits, has various pharmacological benefits such as anti-inflammatory and anti-cancer activities. In the present study, we investigated the anti-cancer mechanism of PEC induced cell death caused by autophagy and apoptosis in AGS and MKN28 human gastric cancer cells. The PEC treatment significantly inhibited the AGS and MKN28 cell growth in a dose-dependent manner. Further, PEC significantly elevated sub-G1 phase in AGS cells and G2/M phase cell cycle arrest in both AGS and MKN28 cells...
August 8, 2018: Nutrients
Ho Seok Seo, Yoon Ju Jung, Ji Hyun Kim, Cho Hyun Park, Han Hong Lee
BACKGROUND: This study presents the initial feasibility of three-port right-side approach-duet totally laparoscopic distal gastrectomy (R-duet TLDG) with uncut Roux-en-Y (R-Y) reconstruction for the treatment of lower- or middle-third gastric cancer. METHODS: A total of 30 patients who underwent R-duet TLDG with uncut R-Y reconstruction for gastric cancer were enrolled. All patients were treated at the Catholic Medical Center. Reconstructions were performed intracorporeally without special instruments...
September 2018: Journal of Laparoendoscopic & Advanced Surgical Techniques. Part A
Akiko Serizawa, Kiyoaki Taniguchi, Takuji Yamada, Kunihiko Amano, Sho Kotake, Shunichi Ito, Masakazu Yamamoto
BACKGROUND: Although patients with stage IV gastric cancer who respond well to systemic chemotherapy can be treated with gastrectomy, the prognosis of patients with unresectable gastric cancer with para-aortic lymph node metastasis is poor. We herein report a case of remnant gastric cancer with para-aortic lymph node metastasis that was treated with potentially curative conversion surgery after showing a complete response to chemotherapy with S-1 and oxaliplatin (SOX). CASE PRESENTATION: An 81-year-old man was diagnosed with type 3 remnant gastric cancer with giant para-aortic lymph node metastasis, and he received SOX chemotherapy...
August 7, 2018: Surgical Case Reports
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