keyword
MENU ▼
Read by QxMD icon Read
search

Crosslinking

keyword
https://www.readbyqxmd.com/read/28339183/tunable-porosity-of-crosslinked-polyhedral-oligomeric-silsesquioxane-poss-supports-for-palladium-catalyzed-aerobic-alcohol-oxidation-in-water
#1
Preeyanuch Sangtrirutnugul, Thanawat Chaiprasert, Warodom Hunsiri, Thanudkit Jitjaroendee, Patsaya Songkhum, Kritapas Laohhasurayotin, Tanakorn Osotchan, Vuthichai Ervithayasuporn
Polyhedral oligomeric silsesquioxane (POSS)-based materials, poly-POSS-Tn [n = 8 (1), 10 (2), 12 (3), and mix (4)], were prepared in high yields via free radical polymerization of corresponding pure forms of methacrylate-functionalized POSS monomers, MMA-POSS-Tn (n = 8, 10, 12), and the mixture form, MMA-POSS-Tmix. Powder X-ray diffraction (XRD) spectra and BET analysis indicate that 1-4 are amorphous materials with high surface areas (683-839 m2•g-1). The surface areas and total pore volumes follow the trend: poly-POSS-T12 > poly-POSS-T10 > poly-POSS-Tmix > poly-POSS-T8...
March 24, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28335265/green-synthesis-of-hierarchically-structured-silver-polymer-nanocomposites-with-antibacterial-activity
#2
María Jesús Hortigüela, Luis Yuste, Fernando Rojo, Inmaculada Aranaz
The in situ formation of silver nanoparticles (AgNPs) aided by chondroitin sulfate and the preparation of a hierarchically structured silver-polymer nanocomposite with antimicrobial activity is shown. Green synthesis of AgNPs is carried out by thermal treatment (80 and 90 °C) or UV irradiation of a chondroitin sulfate solution containing AgNO₃ without using any further reducing agents or stabilizers. Best control of the AgNPs size and polydispersity was achieved by UV irradiation. The ice-segregation-induced self-assembly (ISISA) process, in which the polymer solution containing the AgNPs is frozen unidirectionally, and successively freeze-drying were employed to produce the chondroitin sulfate 3D scaffolds...
July 25, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28335230/fluorescent-nanocomposite-of-embedded-ceria-nanoparticles-in-crosslinked-pva-electrospun-nanofibers
#3
Nader Shehata, Soha Gaballah, Effat Samir, Aya Hamed, Marwa Saad
This paper introduces a new fluorescent nanocomposite of electrospun biodegradable nanofibers embedded with optical nanoparticles. In detail, this work introduces the fluorescence properties of PVA nanofibers generated by the electrospinning technique with embedded cerium oxide (ceria) nanoparticles. Under near-ultra violet excitation, the synthesized nanocomposite generates a visible fluorescent emission at 520 nm, varying its intensity peak according to the concentration of in situ embedded ceria nanoparticles...
June 1, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28334977/sclip-an-integrated-platform-to-study-rna-protein-interactomes-in-biomedical-research-identification-of-cstf2tau-in-alternative-processing-of-small-nuclear-rnas
#4
Yulia Kargapolova, Michal Levin, Karl Lackner, Sven Danckwardt
RNA-binding proteins (RBPs) are central for gene expression by controlling the RNA fate from birth to decay. Various disorders arising from perturbations of RNA-protein interactions document their critical function. However, deciphering their function is complex, limiting the general functional elucidation of this growing class of proteins and their contribution to (patho)physiology. Here, we present sCLIP, a simplified and robust platform for genome-wide interrogation of RNA-protein interactomes based on crosslinking-immunoprecipitation and high-throughput sequencing...
February 28, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28332831/layer-by-layer-assembly-of-amine-reactive-multilayers-using-an-azlactone-functionalized-polymer-and-small-molecule-diamine-linkers
#5
Yashira M Zayas-Gonzalez, Benjamin J Ortiz, David M Lynn
We report the reactive layer-by-layer assembly of amine-reactive polymer multilayers using an azlactone-functionalized polymer and small-molecule diamine linkers. This approach yields crosslinked polymer/linker-type films that can be further functionalized, after fabrication, by treatment with functional primary amines, and provides opportunities to incorporate other useful functionality that can be difficult to introduce using other polyamine building blocks. Films fabricated using poly(2-vinyl-4,4-dimethylazlactone) (PVDMA) and three model non-degradable aliphatic diamine linkers yielded reactive thin films that were stable upon incubation in physiologically relevant media...
March 23, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28332816/fluorinated-and-thermo-crosslinked-polyhedral-oligomeric-silsesquioxanes-new-organic-inorganic-hybrid-materials-for-high-performance-dielectric-application
#6
Jiajia Wang, Jing Sun, Junfeng Zhou, Kaikai Jin, Qiang Fang
A fluorinated and thermo-crosslinked polyhedral oligomeric silsesquioxane (POSS) has been successfully synthesized by thermal polymerization of a fluorinated POSS monomer having an inorganic silsesquioxane core and organic side chains bearing thermo-crosslinkable trifluorovinyl ether groups. This new inorganic-organic hybrid polymer shows high thermostability with a 5 wt% loss temperature of 436 C, as well as good transparency (a sheet with an average thickness of 1.5 mm shows high transmittance of 92% varying from 400 to 1,100 nm)...
March 23, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28332252/functional-organic-material-for-roxarsone-and-its-derivatives-recognition-via-molecular-imprinting
#7
Weigang Fan, Xuemin Zhang, Yagang Zhang, Penglei Wang, Letao Zhang, Zhaoming Yin, Jun Yao, Wei Xiang
Roxarsone, one of feed add drugs containing arsenic, has been most widely used in poultry and swine industry. Roxarsone discharged into the environment has caused serious pollution problem. Herein, a reusable functional material for selective recognition and adsorption of roxarsone and its derivatives were designed and synthesized. The interaction mechanism is based on acid-base interaction and surface molecular imprinting. Dual functionalized core-shell structure with silica gel as the core was prepared to use as carrier for surface molecularly imprinted polymers...
March 22, 2017: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/28330605/polyubiquitin-photoactivatable-crosslinking-reagents-for-mapping-ubiquitin-interactome-identify-rpn1-as-a-proteasome-ubiquitin-associating-subunit
#8
Michal Chojnacki, Wissam Mansour, Dharjath S Hameed, Rajesh K Singh, Farid El Oualid, Rina Rosenzweig, Mark A Nakasone, Zanlin Yu, Fabian Glaser, Lewis E Kay, David Fushman, Huib Ovaa, Michael H Glickman
Ubiquitin (Ub) signaling is a diverse group of processes controlled by covalent attachment of small protein Ub and polyUb chains to a range of cellular protein targets. The best documented Ub signaling pathway is the one that delivers polyUb proteins to the 26S proteasome for degradation. However, studies of molecular interactions involved in this process have been hampered by the transient and hydrophobic nature of these interactions and the lack of tools to study them. Here, we develop Ub-phototrap (Ub(PT)), a synthetic Ub variant containing a photoactivatable crosslinking side chain...
March 16, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28329693/lanthanide-integrated-supramolecular-polymeric-nanoassembly-with-multiple-regulation-characteristics-for-multidrug-resistant-cancer-therapy
#9
Weihong Jin, Qiwen Wang, Min Wu, Yang Li, Guping Tang, Yuan Ping, Paul K Chu
Cancer treatment can in principle be enhanced by the synergistic effects of chemo- and nucleic acid-based combination therapies but the lack of efficient drug nanocarriers and occurrence of multidrug resistance (MDR) are major obstacles adversely affecting the effectiveness. Herein, a lanthanide-integrated supramolecular polymeric nanoassembly that delivers anticancer drugs and siRNA for more effective cancer therapy is described. This nanotherapeutic system is prepared by loading adamantane-modified doxorubicin (Dox) into polyethylenimine-crosslinked-γ-cyclodextrin (PC) through the supramolecular assembly to form the interior Dox-loaded PC (PCD) followed by electrostatically driven self-assembly of siRNA and PCD to produce the PCD/siRNA nanocomplexes...
March 16, 2017: Biomaterials
https://www.readbyqxmd.com/read/28329689/the-human-cchc-type-zinc-finger-nucleic-acid-binding-protein-binds-g-rich-elements-in-target-mrna-coding-sequences-and-promotes-translation
#10
Daniel Benhalevy, Sanjay K Gupta, Charles H Danan, Suman Ghosal, Hong-Wei Sun, Hinke G Kazemier, Katrin Paeschke, Markus Hafner, Stefan A Juranek
The CCHC-type zinc finger nucleic acid-binding protein (CNBP/ZNF9) is conserved in eukaryotes and is essential for embryonic development in mammals. It has been implicated in transcriptional, as well as post-transcriptional, gene regulation; however, its nucleic acid ligands and molecular function remain elusive. Here, we use multiple systems-wide approaches to identify CNBP targets and function. We used photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) to identify 8,420 CNBP binding sites on 4,178 mRNAs...
March 21, 2017: Cell Reports
https://www.readbyqxmd.com/read/28329418/covalent-crosslinking-of-graphene-quantum-dots-by-mcmurry-deoxygenation-coupling
#11
Shaowei Chen, Limei Chen, Peiguangf Hu, Jia En Lu
Graphene quantum dots were covalently crosslinked forming ensembles of a few hundred nanometers in size by McMurry deoxygenation coupling reactions of peripheral carbonyl functional moieties catalyzed by TiCl4 and Zn powders in refluxing THF, as manifested in TEM, AFM, FTIR, Raman and XPS measurements. Photoluminescence measurements showed that after chemical coupling, the excitation and emisison peaks blue-shifted somewhat and the emission intensity increased markedly, likely due to the removal of oxygenated species where quinone-like species were known to be effective electron-acceptors and emission quenchers...
March 22, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28328078/cell-fixation-by-light-triggered-release-of-glutaraldehyde
#12
Korwin M Schelkle, Christopher Schmid, Klaus Yserentant, Markus Bender, Irene Wacker, Martin Petzoldt, Manuel Hamburger, Dirk-Peter Herten, Richard Wombacher, Rasmus R Schröder, Uwe H F Bunz
Chemical fixation of living cells for microscopy is commonly achieved by crosslinking of intracellular proteins with dialdehydes prior to examination. We herein report a photocleavable protecting group for glutaraldehyde that results in a light-triggered and membrane-permeable fixative, which is nontoxic prior to photocleavage. Lipophilic ester groups allow for diffusion across the cell membrane and intracellular accumulation after enzymatic hydrolysis. Irradiation with UV light releases glutaraldehyde. The in situ generated fixative crosslinks intracellular proteins and preserves and stabilizes the cell so that it is ready for microscopy...
March 22, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28327670/global-identification-and-analysis-of-isozyme-specific-possible-substrates-crosslinked-by-transglutaminases-using-substrate-peptides-in-mouse-liver-fibrosis
#13
Hideki Tatsukawa, Yuji Tani, Risa Otsu, Haruka Nakagawa, Kiyotaka Hitomi
The transglutaminase (TG) family comprises eight isozymes that form the isopeptide bonds between glutamine and lysine residues and contribute to the fibrotic diseases via crosslinking-mediated stabilization of ECM and the activation of TGF-β in several tissues. However, despite a growing body of evidence implicating TG2 as a key enzyme in fibrosis, the causative role of TG2 and the involvement of the other isozymes have not yet been fully elucidated. Therefore, here we clarified the distributions of TG isozymes and their in situ activities and identified the isozyme-specific possible substrates for both TG1 and TG2 using their substrate peptides in mouse fibrotic liver...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28327584/hybrid-core-shell-hycos-nanoparticles-produced-by-complex-coacervation-for-multimodal-applications
#14
D Vecchione, A M Grimaldi, E Forte, Paolo Bevilacqua, P A Netti, E Torino
Multimodal imaging probes can provide diagnostic information combining different imaging modalities. Nanoparticles (NPs) can contain two or more imaging tracers that allow several diagnostic techniques to be used simultaneously. In this work, a complex coacervation process to produce core-shell completely biocompatible polymeric nanoparticles (HyCoS) for multimodal imaging applications is described. Innovations on the traditional coacervation process are found in the control of the reaction temperature, allowing a speeding up of the reaction itself, and the production of a double-crosslinked system to improve the stability of the nanostructures in the presence of a clinically relevant contrast agent for MRI (Gd-DTPA)...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28326727/-development-and-characterization-of-oriented-scaffolds-derived-from-cartilage-extracellular-matrix
#15
Li Kun, Zhao Yanhong, Xu Chen, Wang Lianyong, Yang Qiang, Li Hongfa, Teng Binhong
OBJECTIVE: This study aimed to prepare oriented scaffolds derived from a cartilage extracellular matrix (CECM) and to investigate their physicochemical property and compatibility with adipose-derived stem cells (ADSCs). METHODS: A fresh porcine articular cartilage was cut into pieces. Cartilage nanofibers with diameters of 50-500 nm were collected through homogenization and centrifugation. These nanofibers were then decellularized by using Triton X-100 to produce 6% CECM...
February 1, 2017: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28325905/evidence-for-c1q-mediated-crosslinking-of-cd33-lair-1-inhibitory-immunoreceptors-and-biological-control-of-cd33-lair-1-expression
#16
Myoungsun Son, Betty Diamond, Bruce T Volpe, Cynthia B Aranow, Meggan C Mackay, Frances Santiago-Schwarz
C1q collagen-like region (CLR) engaging and activating the LAIR-1 inhibitory immunoreceptor represents a non-complement mechanism for maintaining immune quiescence. Given the binding promiscuity of C1q's globular region (gC1q), we hypothesized that C1q concurrently associates with distinct inhibitory immunoreceptors to produce C1q-mediated modulatory networking. Like LAIR-1, CD33 inhibitory immunoreceptors are highly expressed on monocytes. Binding CD33 restricts cell activation/differentiation; however, natural ligands for CD33 remain elusive...
March 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28325837/threonine-eliminylation-by-bacterial-phosphothreonine-lyases-rapidly-causes-crosslinking-of-mapk-in-live-cells
#17
Benoit M Meijer, Suk Min Jang, Ida C Guerrera, Cerina Chhuon, Joanna Lipecka, Caroline Reisacher, Françoise Baleux, Philippe Sansonetti, Christian Muchardt, Laurence Arbibe
Old long-lived proteins contain dehydroalanine (Dha) and dehydrobutyrine (Dhb), two amino acids engendered by dehydration of serines and threonines respectively. While these residues have a suspected role in protein crosslink and aggregation, their direct implication has yet to be determined. Here, we have taken advantage of the ability of the enteropathogen Shigella to convert the phosphothreonine residue of the pT-X-pY consensus sequence of ERK and p38 into Dhb, and followed the impact of dehydration on the fate of the two MAPKs...
March 21, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28325834/structural-model-of-dodecameric-heat-shock-protein-hsp21-flexible-n-terminal-arms-interact-with-client-proteins-while-c-terminal-tails-maintain-the-dodecamer-and-chaperone-activity
#18
Gudrun Rutsdottir, Johan Härmark, Yoran Weide, Hans Hebert, Morten Ib Rasmussen, Sven Wernersson, Michal Respondek, Mikael Akke, Peter Højrup, Philip J B Koeck, Christopher A G Söderberg, Cecilia Emanuelsson
Small heat shock proteins (sHsps) prevent aggregation of thermosensitive client proteins in a first line of defense against cellular stress. The mechanisms by which they perform this function have been hard to define due to limited structural information; currently there is only one high-resolution structure of a plant sHsp published, of the cytosolic Hsp16.9. We took interest in Hsp21, a chloroplast-localized sHsp crucial for plant stress resistance, which has even longer N-terminals arms than Hsp16.9, with a functionally important and conserved methionine-rich motif...
March 21, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28325781/hete-signals-through-g-protein-coupled-receptor-gpr75-gq-to-affect-vascular-function-and-trigger-hypertension
#19
Victor Garcia, Ankit Gilani, Brian Shkolnik, Varunkumar Pandey, Frank F Zhang, Rambabu Dakarapu, Shyam K Gandham, N R Reddy, Joan P Graves, Artiom Gruzdev, Darryl C Zeldin, Jorge H Capdevila, John R Falck, Michal L Schwartzman
Rationale: 20-Hydroxyeicosatetraenoic acid (20-HETE), one of the principle cytochrome P450 (CYP) eicosanoids, is a potent vasoactive lipid whose vascular effects include stimulation of smooth muscle contractility, migration and proliferation, as well as endothelial cell dysfunction and inflammation. Increased levels of 20-HETE in experimental animals and in humans are associated with hypertension, stroke, myocardial infarction and vascular diseases. Objective: To date, a receptor/binding site for 20-HETE has been implicated based on the use of specific agonists and antagonists...
March 21, 2017: Circulation Research
https://www.readbyqxmd.com/read/28325334/citric-acid-crosslinked-%C3%AE-cyclodextrin-carboxymethylcellulose-hydrogel-films-for-controlled-delivery-of-poorly-soluble-drugs
#20
Vishwajeet Sampatrao Ghorpade, Adhikrao Vyankatrao Yadav, Remeth Jacky Dias
Citric acid crosslinked β-cyclodextrin-carboxymethylcellulose (βCD-CMC) hydrogel films were prepared by esterification-crosslinking method for the controlled release of ketoconazole (model drug). The hydrogel films were evaluated for active βCD content, carboxyl content, swelling ratio, drug loading and release, and hemolytic activity. The structural characterization was carried out using solid state (13)C NMR, ATR-FTIR, TGA and DSC analysis. The βCD-CMC hydrogel films showed increase in active βCD content swelling ratio and drug loading with increase in the concentration of βCD in the feed...
May 15, 2017: Carbohydrate Polymers
keyword
keyword
16589
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"