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https://www.readbyqxmd.com/read/28094043/incidence-and-clinical-course-of-immune-reactions-after-descemet-membrane-endothelial-keratoplasty-retrospective-analysis-of-1000-consecutive-eyes
#1
Deniz Hos, Onur Tuac, Friederike Schaub, Tisha P Stanzel, Silvia Schrittenlocher, Martin Hellmich, Björn O Bachmann, Claus Cursiefen
PURPOSE: To analyze the incidence and clinical course of graft rejection episodes after Descemet membrane endothelial keratoplasty (DMEK). DESIGN: Retrospective analysis of a consecutive, interventional case series. PARTICIPANTS: One thousand eyes that underwent DMEK from July 2011 through August 2015 at the Department of Ophthalmology, University of Cologne. METHODS: All cases with follow-up of at least 1 month were included (mean follow-up, 18...
January 13, 2017: Ophthalmology
https://www.readbyqxmd.com/read/28079691/lutein-and-brilliant-blue-based-dye-for-donor-preparation-and-transplantation-in-descemet-membrane-endothelial-keratoplasty
#2
Sebastian Siebelmann, Mario Matthaei, Robert Hörster, Claus Cursiefen, Björn Bachmann
PURPOSE: Trypan blue (TB) is used for visualization of the endothelium-Descemet membrane graft during Descemet membrane endothelial keratoplasty (DMEK). However, TB is assumed to have a dose-dependent toxic effect on the corneal endothelium. We retrospectively compared a lutein/zeaxanthin and Brilliant Blue (LZ/BB)-based dye for preparation and transplantation of the graft in DMEK to TB. METHODS: In 20 patients, a dye composed of 1% LZ /0.025% BB was used, and in 22 patients, 0...
January 11, 2017: Cornea
https://www.readbyqxmd.com/read/28079689/scanning-electron-microscope-analysis-of-stromal-surface-regularity-in-dsaek-using-manual-or-automated-microkeratomes-or-dmek
#3
Alberto Villarrubia, Enrique García, Antonio Cano-Ortiz, Alberto Membrillo, Antoni Sabala, Javier Celis, Antoni Salvador, Jorge Solana, Alexandra Arango, Irene Sassot
PURPOSE: To analyze differences in the stromal bed according to the method used to obtain the disc for endothelial transplant with 1) an automated microkeratome for Descemet stripping automated endothelial keratoplasty (DSAEK), 2) homogeneous dissection of Descemet membrane (Descemet membrane endothelial keratoplasty [DMEK]), or 3) a manual microkeratome used for DSAEK. METHODS: We analyzed the stromal bed of 25 consecutive corneas used for endothelial transplantation, divided into 3 groups: 1) 11 samples cut with an automated microkeratome, 2) 5 samples used for DMEK, 3) 9 samples cut with a manual microkeratome...
January 11, 2017: Cornea
https://www.readbyqxmd.com/read/28060074/eventual-endothelial-failure-after-initial-corneal-clearing-after-a-detached-endothelial-graft-in-fuchs-dystrophy
#4
Hyunjoo J Lee, Wuqaas M Munir
PURPOSE: To report a case of eventual corneal endothelial cell failure after spontaneous resolution of corneal edema after failed Descemet membrane endothelial keratoplasty (DMEK). METHODS: Retrospective case report. RESULTS: A 56-year-old man with Fuchs endothelial corneal dystrophy underwent cataract and DMEK surgery in the left eye. The transplanted DMEK graft detached in the early postoperative period and formed a peripheral scroll. Despite graft detachment, corneal edema resolved with endothelial cell repopulation of the central cornea...
February 2017: Cornea
https://www.readbyqxmd.com/read/28060070/enhancing-dmek-success-by-identifying-optimal-levels-of-trypan-blue-dye-application-to-donor-corneal-tissue
#5
Parag A Majmudar, Lauren Johnson
PURPOSE: The purpose of this study was to determine the correct concentration and duration of application of trypan blue dye to Descemet membrane endothelial keratoplasty (DMEK) donor corneal tissue before transplant to enhance visibility while minimizing damage to the tissue. METHODS: Corneas were precut for DMEK using an 8-mm punch, and each was stained with either 0.06% or 0.15% trypan blue for a duration of 1, 3, or 5 minutes. After staining, each tissue was placed in balanced salt solution and observed to determine how quickly the stain faded from the tissue using a grading system...
February 2017: Cornea
https://www.readbyqxmd.com/read/28018708/clinical-outcomes-of-descemet-membrane-endothelial-keratoplasty-during-the-surgeon-learning-curve-versus-descemet-stripping-endothelial-keratoplasty-performed-at-the-same-time
#6
Jennifer Rose-Nussbaumer, Shashi Alloju, Winston Chamberlain
PURPOSE: To compare outcomes after Descemet Membrane Endothelial Keratoplasty (DMEK) and traditional Descemet Stripping Endothelial Keratoplasty (DSEK) during the surgeon's DMEK learning curve in a prospective, non-randomized, consecutive, interventional case series. METHODS: Consecutive patients presenting to the university eye clinics and undergoing endothelial keratoplasty were included. Data including patient demographics, visual acuity, endothelial cell counts and complications were recorded at baseline, as well as 3 and 6 months post-operatively...
October 2016: Journal of Clinical & Experimental Ophthalmology
https://www.readbyqxmd.com/read/28009413/role-of-dextran-in-maintaining-adhesive-and-stiffness-properties-of-prestripped-dmek-lenticules
#7
Mohit Parekh, Alessandro Ruzza, Rosa Di Mundo, Stefano Ferrari, Giuseppina Recchia, Hossein Elbadawy, Giuseppe Carbone, Diego Ponzin
PURPOSE: To investigate the adhesive and stiffness properties of prestripped Descemet membrane endothelial keratoplasty (DMEK) lenticules in different preservation conditions (with and without dextran). METHODS: The study included 3 conditions: (C1) tissues collected from tissue culture media (TCM), stripped and preserved in TCM; (C2) tissues collected from transport media (TM) (TCM supplemented with 6% dextran T-500), stripped and preserved in TM; and (C3) tissues collected from TCM, stripped and preserved in TM...
November 25, 2016: European Journal of Ophthalmology
https://www.readbyqxmd.com/read/28002113/techniques-for-learning-descemet-membrane-endothelial-keratoplasty-for-eyes-of-asian-patients-with-shallow-anterior-chamber
#8
Takahiko Hayashi, Itaru Oyakawa, Naoko Kato
PURPOSE: To describe several essential surgical techniques that overcome difficulties in performing Descemet membrane endothelial keratoplasty (DMEK) for inexperienced surgeons, especially those who perform DMEK on eyes of Asian patients. METHODS: Nine eyes of 9 Asian patients with bullous keratopathy who underwent DMEK were analyzed retrospectively. All patients were given a diuretic such as D-mannitol or acetazolamide shortly before surgery, with retrobulbar anesthesia and a Nadbath facial nerve block...
January 4, 2017: Cornea
https://www.readbyqxmd.com/read/28002107/astigmatism-correction-with-toric-intraocular-lenses-in-descemet-membrane-endothelial-keratoplasty-triple-procedures
#9
Hideaki Yokogawa, P James Sanchez, Zachary M Mayko, Michael D Straiko, Mark A Terry
PURPOSE: To report the clinical efficacy of astigmatism correction with toric intraocular lenses (IOLs) in patients undergoing the Descemet membrane endothelial keratoplasty (DMEK) triple procedure and to evaluate the accuracy of the correction. METHODS: Fifteen eyes of 10 patients who received cataract extraction, toric IOL placement, and DMEK surgery for Fuchs corneal dystrophy and cataracts were evaluated. The cylinder power of toric IOLs was determined by an online toric calculator with keratoscopy measurements obtained using Scheimpflug corneal imaging...
December 20, 2016: Cornea
https://www.readbyqxmd.com/read/27994884/preservation-of-preloaded-dmek-lenticules-in-dextran-and-non-dextran-based-organ-culture-medium
#10
Mohit Parekh, Alessandro Ruzza, Stefano Ferrari, Diego Ponzin
Purpose. To determine the optimum preservation conditions for preloading DMEK lenticules using organ culture system. Methods. 8.5 mm DMEK lenticules were stripped and preserved with endothelium flap-in for 4 days at RT in an IOL cartridge that was blocked with rubber stoppers from each end. In C1, tissues were collected from tissue culture medium (TCM) and preserved in TCM. In C2, tissues were collected from transport medium (TCM + 6% dextran T500) (TM) and preserved in TM. In C3, tissues were collected from TCM and preserved in TM...
2016: Journal of Ophthalmology
https://www.readbyqxmd.com/read/27994805/descemet-s-membrane-endothelial-keratoplasty-in-south-asian-population
#11
Vipul Bhandari, Jagdeesh K Reddy, Prateek Chougale
PURPOSE: To evaluate visual outcomes, endothelial cell density and complications following Descemet's membrane endothelial keratoplasty (DMEK). METHODS: This study included 40 consecutive eyes that underwent DMEK for various pathologies involving the corneal endothelium. Best corrected visual acuity (BCVA) and endothelial cell density (ECD) were measured and compared before and 6 months after surgery. RESULTS: Out of 40 eyes, 34 eyes (85%) had BCVA ranging from 0...
October 2016: Journal of Ophthalmic & Vision Research
https://www.readbyqxmd.com/read/27994800/dmek-calling
#12
EDITORIAL
Alfonso Vasquez Perez, Mehran Zarei-Ghanavati, Christopher Liu
No abstract text is available yet for this article.
October 2016: Journal of Ophthalmic & Vision Research
https://www.readbyqxmd.com/read/27986425/two-year-course-of-corneal-densitometry-after-descemet-membrane-endothelial-keratoplasty-dmek
#13
Friederike Schaub, Philip Enders, Cornelius Bluhm, Björn O Bachmann, Claus Cursiefen, Ludwig M Heindl
PURPOSE: To investigate the two-year course of corneal densitometry following Descemet membrane endothelial keratoplasty (DMEK) for Fuchs endothelial dystrophy (FED). DESIGN: Retrospective, interventional case series. METHODS: Densitometry values (corneal light backscatter in greyscale units) measured by Scheimpflug-based imaging and clinical records of 160 eyes of 160 patients undergoing DMEK for FED were reviewed. Outcome measures included densitometry data in 4 corneal layers (anterior layer (AL), central layer (CL), posterior layer (PL), and total layer (TL)) and 2 annuli (central annular zone 0-2mm and peripheral zone 2-10mm), central corneal thickness (CCT), best spectacle-corrected visual acuity (BSCVA), and endothelial cell density (ECD) prior to DMEK and at 1, 3, 6, 12, and 24 months postoperatively...
December 13, 2016: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/27984366/human-ex-vivo-artificial-anterior-chamber-model-for-practice-dmek-surgery
#14
Alfonso Vasquez Perez, Christopher Liu
PURPOSE: To describe a wet lab model using human corneas for practicing all steps of Descemet membrane endothelial keratoplasty (DMEK). METHODS: Twelve human research corneas were used for training purposes. Each DMEK surgery required 2 corneas; one for donor graft preparation and one to practice graft insertion and unfolding in a novel artificial anterior chamber model. RESULTS: Six complete DMEK procedures were successfully performed. Apart from donor graft preparation and graft insertion into the anterior chamber, it was feasible to practice different and combinations of graft unfolding techniques in conditions similar to real human procedures...
December 14, 2016: Cornea
https://www.readbyqxmd.com/read/27982207/comparison-of-20-sulfur-hexafluoride-with-air-for-intraocular-tamponade-in-descemet-membrane-endothelial-keratoplasty-dmek
#15
Benjamin Botsford, Gustavo Vedana, Leslie Cope, Samuel C Yiu, Albert S Jun
Purpose: To compare the effect of 20% sulfur hexafluoride (SF6) with that of air on graft detachment rates for intraocular tamponade in Descemet membrane endothelial keratoplasty (DMEK). Methods: Forty-two eyes of patients who underwent DMEK by a single surgeon (A.S.J.) at Wilmer Eye Institute between January 2012 and 2014 were identified; 21 received air for intraocular tamponade and the next consecutive 21 received SF6. The main outcome measure was the graft detachment rate; univariate and multivariate analyses were performed...
September 2016: Arquivos Brasileiros de Oftalmologia
https://www.readbyqxmd.com/read/27974291/organ-cultured-prestripped-donor-tissue-for-dmek-surgery-clinical-outcomes
#16
Johannes Menzel-Severing, Friedrich E Kruse, Theofilos Tourtas
AIM: To determine whether clinical performance is negatively affected by prestripping Descemet membrane endothelial keratoplasty (DMEK) grafts from organ-cultured corneas. METHODS: We reviewed clinical records of all patients who underwent DMEK surgery for Fuchs endothelial dystrophy between 28 October 2014 and 11 August 2015. Grafts had been prepared from organ-cultured corneoscleral buttons 24 hours prior to surgery or during surgery. We included only patients for which at least one follow-up examination was available at a minimum of 2 months postoperatively...
December 14, 2016: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/27941386/ten-year-clinical-outcome-of-the-first-patient-undergoing-descemet-membrane-endothelial-keratoplasty
#17
Lamis Baydoun, Thomas Müller, Itay Lavy, Jack Parker, Marina Rodriguez-Calvo-de-Mora, Vasilios S Liarakos, Isabel Dapena, Gerrit R J Melles
PURPOSE: To describe the 10-year clinical outcome of the first patient worldwide who underwent Descemet membrane endothelial keratoplasty (DMEK). METHODS: In 2006, a 63-year-old man presented at the Melles Cornea Clinic, Rotterdam, with bilateral Fuchs endothelial dystrophy and cataract. After phacoemulsification, in vivo DMEK was performed in the left eye and 10 months later in the right eye. Best spectacle-corrected visual acuity (BSCVA), endothelial cell density, pachymetry, and complications were recorded every 6 months over a 10-year period...
December 8, 2016: Cornea
https://www.readbyqxmd.com/read/27938949/dual-purpose-corneal-tissue-for-anterior-lamellar-keratoplasty-and-descemet-s-membrane-endothelial-keratoplasty
#18
C Larena Menant-Tay, Ronan Conlon, Salina Teja, Season Yeung, Joshua Teichman, Setareh Ziai, Kashif Baig
OBJECTIVE: To assess the intraoperative issues and surgical outcomes of preparing a single-donor corneal tissue for same-day use in both deep anterior lamellar keratoplasty (DALK) and Descemet's membrane endothelial keratoplasty (DMEK). DESIGN: Consecutive retrospective case series. PARTICIPANTS: Ten eyes of 10 patients who underwent DALK (5 patients) or DMEK (5 patients) surgery using dual-purpose corneal tissue. METHODS: Five dual-purpose corneoscleral rims were used to prepare tissue for 5 DMEK and 5 DALK procedures...
December 2016: Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie
https://www.readbyqxmd.com/read/27918355/experienced-dsaek-surgeon-s-transition-to-dmek-outcomes-comparing-the-last-100-dsaek-surgeries-with-the-first-100-dmek-surgeries-exclusively-using-previously-published-techniques
#19
Paul M Phillips, Louis J Phillips, Valliamai Muthappan, Charlene M Maloney, Christopher N Carver
PURPOSE: To compare outcomes of Descemet stripping automated endothelial keratoplasty (DSAEK) and Descemet membrane endothelial keratoplasty (DMEK) when an experienced DSAEK surgeon transitions to DMEK while following 2 published endothelial keratoplasty surgical techniques. METHODS: This is a retrospective review of 200 eyes of 132 patients with Fuchs corneal dystrophy that underwent endothelial keratoplasty performed by one surgeon. Published, standardized techniques were followed for both DSAEK and DMEK...
December 1, 2016: Cornea
https://www.readbyqxmd.com/read/27906816/intraocular-pressure-measurements-after-descemet-membrane-endothelial-keratoplasty
#20
Anna-Karina B Maier, Enken Gundlach, Milena Pahlitzsch, Johannes Gonnermann, Caitlin Corkhill, Eckart Bertelmann, Antonia M Joussen, Matthias K J Klamann, Necip Torun
PURPOSE: Intraocular pressure (IOP) elevation occurs regularly after Descemet membrane endothelial keratoplasty (DMEK). This study evaluated the effect of central corneal thickness (CCT) on the IOP after DMEK. PATIENTS AND METHODS: This prospective study recorded the IOP of 46 eyes from 46 patients preoperatively, and then 1 and 3 months after DMEK. IOP measurement was performed by noncontact pneumatic tonometry (NCT), iCare, Goldmann applanation tonometry (GAT), and dynamic contour tonometry (DCT)...
November 30, 2016: Journal of Glaucoma
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