Cheryl Cero, Weiguo Shu, Amy L Reese, Diana Douglas, Michael Maddox, Ajeet P Singh, Sahara L Ali, Alexander R Zhu, Jacqueline M Katz, Anne E Pierce, Kelly T Long, Naris Nilubol, Raymond H Cypess, Jonathan L Jacobs, Fang Tian, Aaron M Cypess
Functional human brown and white adipose tissue (BAT and WAT) are vital for thermoregulation and nutritional homeostasis, while obesity and other stressors lead, respectively, to cold intolerance and metabolic disease. Understanding BAT and WAT physiology and dysfunction necessitates clinical trials complemented by mechanistic experiments at the cellular level. These require standardized in vitro models, currently lacking, that establish references for gene expression and function. In response, we generated and characterized a pair of immortalized, clonal human brown (hBA) and white (hWA) preadipocytes derived from the perirenal and subcutaneous depots, respectively, of a 40-year-old male...
November 7, 2023: Endocrinology