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https://www.readbyqxmd.com/read/30033170/comparison-of-two-low-flow-interfaces-for-measurement-of-mobilities-and-stability-constants-by-affinity-capillary-electrophoresis-mass-spectrometry
#1
Renáta Konášová, Dušan Koval, Jana Jaklová Dytrtová, Václav Kašička
Affinity capillary electrophoresis (ACE) is typically used for the determination of stability constant, Kst , of weak to moderately strong complexes. Sensitive detection such as mass spectrometry (MS) is required for extension of ACE methodology for estimation of Kst of stronger complexes. Consequently, an efficient interface for hyphenation of CE with MS detection is necessary. For evaluation of interfaces for electrospray ionization mass spectrometric (ESI/MS) detection in ACE conditions, potassium-crown ether complexation was used as model system...
July 19, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29709640/metagenome-based-diversity-analyses-suggest-a-strong-locality-signal-for-bacterial-communities-associated-with-oyster-aquaculture-farms-in-ofunato-bay
#2
Atsushi Kobiyama, Kazuho Ikeo, Md Shaheed Reza, Jonaira Rashid, Yuichiro Yamada, Yuri Ikeda, Daisuke Ikeda, Nanami Mizusawa, Shigeru Sato, Takehiko Ogata, Mitsuru Jimbo, Toshiaki Kudo, Shinnosuke Kaga, Shiho Watanabe, Kimiaki Naiki, Yoshimasa Kaga, Katsuhiko Mineta, Vladimir Bajic, Takashi Gojobori, Shugo Watabe
Ofunato Bay, in Japan, is the home of buoy-and-rope-type oyster aquaculture activities. Since the oysters filter suspended materials and excrete organic matters into the seawater, bacterial communities residing in its vicinity may show dynamic changes depending on the oyster culture activities. We employed a shotgun metagenomic technique to study bacterial communities near oyster aquaculture facilities at the center of the bay (KSt. 2) and compared the results with those of two other localities far from the station, one to the northeast (innermost bay, KSt...
July 30, 2018: Gene
https://www.readbyqxmd.com/read/29709637/seasonal-changes-in-the-communities-of-photosynthetic-picoeukaryotes-in-ofunato-bay-as-revealed-by-shotgun-metagenomic-sequencing
#3
Jonaira Rashid, Atsushi Kobiyama, Md Shaheed Reza, Yuichiro Yamada, Yuri Ikeda, Daisuke Ikeda, Nanami Mizusawa, Kazuho Ikeo, Shigeru Sato, Takehiko Ogata, Toshiaki Kudo, Shinnosuke Kaga, Shiho Watanabe, Kimiaki Naiki, Yoshimasa Kaga, Katsuhiko Mineta, Vladimir Bajic, Takashi Gojobori, Shugo Watabe
Small photosynthetic eukaryotes play important roles in oceanic food webs in coastal regions. We investigated seasonal changes in the communities of photosynthetic picoeukaryotes (PPEs) of the class Mamiellophyceae, including the genera Bathycoccus, Micromonas and Ostreococcus, in Ofunato Bay, which is located in northeastern Japan and faces the Pacific Ocean. The abundances of PPEs were assessed over a period of one year in 2015 at three sampling stations, KSt. 1 (innermost bay area), KSt. 2 (middle bay area) and KSt...
July 30, 2018: Gene
https://www.readbyqxmd.com/read/29706796/measuring-extended-families-over-time-in-informal-settlements-in-nairobi-kenya-retention-and-data-consistency-in-a-two-round-survey
#4
Sangeetha Madhavan, Donatien Beguy, Shelley Clark, Caroline Kabiru
BACKGROUND: Researchers have increasingly turned to longitudinal data to understand how the family environment of children changes over time and how this change affects their well-being. While the value of such efforts is clear, the inherent challenges of collecting robust data over time may limit or bias our understanding of family complexity. OBJECTIVE: Drawing on data from an exploratory study on kinship structure and support for low income single mothers and their young children in Nairobi, Kenya, this paper aims to (1) assess the strengths and weaknesses of our approach in reflecting the complexities of kinship dynamics and (2) analyze how methodological issues such as selection and reporting inconsistency can influence our understanding of the role of kin in children's lives...
January 2018: Demographic Research
https://www.readbyqxmd.com/read/29485150/calculation-of-linear-and-nonlinear-optical-properties-of-azobenzene-derivatives-with-kohn-sham-and-coupled-cluster-methods
#5
Arun K Pal, Thomas J Duignan, Jochen Autschbach
Linear polarizabilities (α) and second hyperpolarizabilities (γ) of unsubstituted azobenzenes and 'push-pull' azobenzene derivatives are investigated using Kohn-Sham theory (KST) and coupled-cluster (CC) approaches. Various standard exchange-correlation functionals as well as a non-empirically 'tuned' long-range corrected (LC) functional with range-separated exchange are used in the KST calculations. When compared to correlated ab initio calculations and measurements, the tuned functional gives accurate low-energy excitation energies, especially for charge transfer (CT) transitions, and performs well for α...
March 7, 2018: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29326287/the-apical-scaffold-big-bang-binds-to-spectrins-and-regulates-the-growth-of-drosophila-melanogaster-wing-discs
#6
Elodie Forest, Rémi Logeay, Charles Géminard, Diala Kantar, Florence Frayssinoux, Lisa Heron-Milhavet, Alexandre Djiane
During development, cell numbers are tightly regulated, ensuring that tissues and organs reach their correct size and shape. Recent evidence has highlighted the intricate connections between the cytoskeleton and the regulation of the key growth control Hippo pathway. Looking for apical scaffolds regulating tissue growth, we describe that Drosophila melanogaster big bang (Bbg), a poorly characterized multi-PDZ scaffold, controls epithelial tissue growth without affecting epithelial polarity and architecture...
March 5, 2018: Journal of Cell Biology
https://www.readbyqxmd.com/read/29102638/experimental-investigation-of-the-inerting-effect-of-crystalline-ii-type-ammonium-polyphosphate-on-explosion-characteristics-of-micron-size-acrylates-copolymer-dust
#7
Yuan Yu, Yunhao Li, Qingwu Zhang, Weishun Ni, Juncheng Jiang
The inerting effect of crystalline II type Ammonium Polyphosphate (APP-II) on explosion characteristics of micron-size Acrylates Copolymer (ACR) powders was experimentally studied. The inerting mechanism was analysed by combining thermogravimetry (TG) and differential scanning calorimetry (DSC) tests. The results indicated that the maximum explosion pressure (Pmax ) and explosion index (Kst ) was 10.4bar and 416bar m/s, respectively for ACR powders. The minimum explosion concentration (MEC) of ACR powders ranged from 20 to 30g/m3 , and the experimental minimum ignition energy (MIE) of the ACR dust cloud was 10mJ...
February 15, 2018: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28913056/the-effects-of-thalidomide-in-a-rat-model-of-surgically-induced-endometriosis
#8
Murat Bakacak, Önder Ercan, Bülent Köstü, Mehmet Sühha Bostancı, Fatma İnanç, Aslı Yaylalı, Salih Serin, Ozan Balakan, Gürkan Kıran
OBJECTIVE: The aim of the study was to analyze the anti-angiogenic role of thalidomide and to assess whether thalidomide had any influence on a rat model of surgically-induced endometriosis. MATERIALS AND METHODS: Endometriosis was induced through surgical induction and homologous transplantation in 16 rats. The rats were randomly separated into two groups as thalidomide (n=8) and control (n=8) groups. Using oral gavage, 100 mg/kg thalidomide 0.5 ml was administered to the first group and saline 0...
September 2015: Turkish Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28600742/does-ventrolateral-prefrontal-cortex-help-in-searching-for-the-lost-key-evidence-from-an-fnirs-study
#9
Marika Carrieri, Stefania Lancia, Alessia Bocchi, Marco Ferrari, Laura Piccardi, Valentina Quaresima
The Key Search Task (KST) is a neuropsychological test that requires strategies for searching a lost key in an imaginary field. This request may involve different cognitive processes as mental imagery and navigation planning. This study was aimed at investigating, by a twenty-channel functional near-infrared spectroscopy (fNIRS) system, the hemodynamic response (i.e., oxygenated-hemoglobin (O2 Hb) and deoxygenated-hemoglobin (HHb) changes) of the prefrontal cortex in navigation planning. A right ventrolateral prefrontal cortex (rVLPFC) activation during the KST was hypothesized...
June 2018: Brain Imaging and Behavior
https://www.readbyqxmd.com/read/28495273/the-key-of-the-maze-the-role-of-mental-imagery-and-cognitive-flexibility-in-navigational-planning
#10
Alessia Bocchi, Marika Carrieri, Stefania Lancia, Valentina Quaresima, Laura Piccardi
Spatial navigation planning ability relies on both mental imagery and cognitive flexibility. Considering the importance of planning ability in everyday life, several neuropsychological tests are used in clinical practice for its assessment, although some of these are not aimed at assessing the strategies of navigational planning. The Porteus Maze Test (PMT) and the Key Search Task (KST) require to plan a strategy in a maze and in an imagined space, respectively. To the best of our knowledge, although these two tests share some features, the relationship between them has never been explored...
June 9, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28376888/daily-administration-of-yokukansan-and-keishito-prevents-social-isolation-induced-behavioral-abnormalities-and-down-regulation-of-phosphorylation-of-neuroplasticity-related-signaling-molecules-in-mice
#11
COMPARATIVE STUDY
Hironori Fujiwara, Yaoyu Han, Ken Ebihara, Suresh Awale, Ryota Araki, Takeshi Yabe, Kinzo Matsumoto
BACKGROUND: Our previous studies demonstrated that post-weaning social isolation (ISO) in mice induces behavior abnormalities such as deficits of sociability- and attention-like behaviors. These deficits can be attenuated by methylphenidate (MPH), a drug used for attention deficit hyperactivity disorder (ADHD), suggesting that ISO mice offer a potential animal model of comorbid developmental disorder with ADHD and autism spectrum disorder symptoms. This study investigated the effects of Kampo formulae, yokukansan (YKS) and keishito (KST), on the neuropsychiatric symptoms of ISO mice to clarify the therapeutic or preventive/delaying potential of these formulae for the treatment of neurodevelopmental disorders...
April 4, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28352297/adaptive-evolution-and-demographic-history-contribute-to-the-divergent-population-genetic-structure-of-potato-virus-y-between-china-and-japan
#12
Fangluan Gao, Wenchao Zou, Lianhui Xie, Jiasui Zhan
Potato virus Y (PVY) is an important plant pathogen causing considerable economic loss to potato production. Knowledge of the population genetic structure and evolutionary biology of the pathogen, particularly at a transnational scale, is limited but vital in developing sustainable management schemes. In this study, the population genetic structure and molecular evolution of PVY were studied using 127 first protein (P1) and 137 coat protein (CP) sequences generated from isolates collected from potato in China and Japan...
April 2017: Evolutionary Applications
https://www.readbyqxmd.com/read/28258978/use-of-gelatin-sponge-affects-postoperative-morbidity-in-cesarean-section-patients
#13
Alev Özer, Bülent Köstü
BACKGROUND This study aimed to determine the effects of use of a local hemostatic gelatin sponge (GS) on postoperative morbidity in patients undergoing cesarean section (CS). MATERIAL AND METHODS The records of 318 patients who underwent CS surgery were retrospectively evaluated. Group 1 consisted of 59 patients with gelatin sponge (GS) applied, and Group 2 consisted of 259 patients with no GS applied. The groups were compared for time to the first flatus, nausea and vomiting, requirement for anti-emetic drugs, development of postoperative ileus, and the length of hospitalization...
March 4, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28238306/effect-of-hydrothermal-treatment-on-light-transmission-of-translucent-zirconias
#14
Armand Putra, Kwok-Hung Chung, Brian D Flinn, Tuesday Kuykendall, Cheng Zheng, Kosuke Harada, Ariel J Raigrodski
STATEMENT OF PROBLEM: Studies of the light transmission of translucent zirconias after hydrothermal treatment are limited. PURPOSE: The purpose of this in vitro study was to evaluate the effect of hydrothermal treatment on the light transmission of translucent zirconias for monolithic restorations. MATERIAL AND METHODS: Four commercially available zirconia products, BruxZir Anterior Solid Zirconia (BruxAnt, BA), Lava Plus High Translucency (LPHT), Katana Zirconia Super Translucent (KST), and Katana Zirconia Ultra Translucent (KUT) were assessed and 1 type of lithium disilicate, e...
September 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28139166/moving-beyond-the-household-innovations-in-data-collection-on-kinship
#15
Sangeetha Madhavan, Shelley Clark, Donatien Beguy, Caroline W Kabiru, Mark Gross
Across settings, it has been shown that the co-residential household is an insufficient measure of family structure and support. However, it continues to be the primary means of population data collection. To address this problem, we developed a new instrument, the Kinship Support Tree (KST), to collect kinship structure and support data on co-residential and non-residential kin and tested it on a sample of 462 single mothers and their children in a slum community in Nairobi, Kenya. This instrument is unique in four important ways: (1) it is not limited to the co-residential household; (2) it distinguishes potential from functional kin; (3) it incorporates multiple geospatial measures; and (4) it collects data on kin relationships specifically for children...
March 2017: Population Studies
https://www.readbyqxmd.com/read/27958630/increased-oxidative-stress-is-associated-with-insulin-resistance-and-infertility-in-polycystic-ovary-syndrome
#16
Alev Özer, Murat Bakacak, Hakan Kıran, Önder Ercan, Bülent Köstü, Mine Kanat-Pektaş, Metin Kılınç, Ferhat Aslan
OBJECTIVES: The present study aims to investigate the role of oxidant-antioxidant status in young women with polycystic ovary syndrome (PCOS). MATERIAL AND METHODS: Seventy-one women with PCOS and 53 healthy controls are compared in aspect of demographic characteristics, biochemical data, hormones, and oxidant-antioxidant status. RESULTS: The PCOS group had significantly lower zinc, higher malondialdehyde and gluthathione peroxidase and lower serum catalase levels than the control group (p = 0...
2016: Ginekologia Polska
https://www.readbyqxmd.com/read/27542094/a-fluorescent-probe-for-imaging-sirtuin-activity-in-living-cells-based-on-one-step-cleavage-of-the-dabcyl-quencher
#17
Mitsuyasu Kawaguchi, Shohei Ikegawa, Naoya Ieda, Hidehiko Nakagawa
Sirtuins (SIRTs) are a family of NAD+ -dependent histone deacetylases. In mammals, dysfunction of SIRTs is associated with age-related metabolic diseases and cancers, so SIRT modulators are considered attractive therapeutic targets. However, current screening methodologies are problematic, and no tools for imaging endogenous SIRT activity in living cells have been available until now. In this work we present a series of simple and highly sensitive new SIRT activity probes. Fluorescence of these probes is activated by SIRT-mediated hydrolytic release of a 4-(4-dimethylaminophenylazo)benzoyl (Dabcyl)-based FRET quencher moiety from the ϵ-amino group of lysine in a nonapeptide derived from histone H3K9 and bearing a C-terminal fluorophore...
October 17, 2016: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27515319/synthesis-and-anticancer-activity-of-a-hydroxytolan-series
#18
Boren Lin, Karen McGuire, Bo Liu, James Jamison, Chun-Che Tsai
This paper describes the development of novel anticancer poly-hydroxylated tolans. Based on structural similarity to resveratrol, a series of hydroxytolans were synthesized and evaluated for their antitumor capability against three tumor cell lines and one fibroblast cell line for selectivity comparisons. The 4,4'-dihydroxytolan (KST-201) exhibited the most significant anticancer activity with increased selectivity when compared to resveratrol and other hydroxytolans. Unlike resveratrol, KST-201 can boost hydrogen peroxide in tumor cells, which are often at high basal level of reactive oxygen species, to cause cell death by overwhelming the cellular tolerance of oxidative stress...
September 15, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27468178/potential-explosion-hazard-of-carbonaceous-nanoparticles-screening-of-allotropes
#19
Leonid A Turkevich, Joseph Fernback, Ashok G Dastidar, Paul Osterberg
There is a concern that engineered carbon nanoparticles, when manufactured on an industrial scale, will pose an explosion hazard. Explosion testing has been performed on 20 codes of carbonaceous powders. These include several different codes of SWCNTs (single-walled carbon nanotubes), MWCNTs (multi-walled carbon nanotubes) and CNFs (carbon nanofibers), graphene, diamond, fullerene, as well as several different control carbon blacks and graphites. Explosion screening was performed in a 20 L explosion chamber (ASTM E1226 protocol), at a concentration of 500 g/m3 , using a 5 kJ ignition source...
May 2016: Combustion and Flame
https://www.readbyqxmd.com/read/26950349/the-kansas-squat-test-modality-comparison-free-weights-vs-smith-machine
#20
COMPARATIVE STUDY
Paul E Luebbers, Andrew C Fry
Luebbers, PE and Fry, AC. The Kansas squat test modality comparison: free weights vs. smith machine. J Strength Cond Res 30(8): 2186-2193, 2016-Standardized methods of testing power are instrumental in planning and implementing training regimens for many athletes, and also in tracking training adaptations. Previous work has demonstrated that the Kansas squat test (KST) is a valid test for measuring indices of mean and peak power when compared with the Wingate anaerobic cycle test. Although the KST was designed for use with a Smith machine (SM), many power athletes use free weights for training...
August 2016: Journal of Strength and Conditioning Research
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