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p38 MAPK AP-1

Trong Thuan Ung, Thi Thinh Nguyen, Sen Lian, Shinan Li, Yong Xia, Nam Ho Kim, Young Do Jung
Interleukin-6 (IL-6), a pleiotropic cytokine, plays a key role in endothelial injury and atherosclerosis. In this study, we investigated the effects of nicotine, a major psychoactive compound in cigarette smoke, on IL-6 expression and EA.hy926 endothelial cell invasion. Nicotine stimulated IL-6 expression via the activator protein 1 (AP-1) transcription factor. Pharmacological inhibition and mutagenesis studies indicated that p38 mitogen-activated protein kinase (MAPK) mediated the IL-6-induced upregulation of nicotine in EA...
October 14, 2018: Journal of Cellular Biochemistry
I-Ta Lee, Chih-Chung Lin, Chien-Chung Yang, Li-Der Hsiao, Ming-Yen Wu, Chuen-Mao Yang
Staphylococcus aureus ( S. aureus ) is a very common Gram-positive bacterium. It is widely distributed in air, soil, and water. S. aureus often causes septicemia and pneumonia in patients. In addition, it is considered to play a key role in mediating cell adhesion molecules upregulation. Resveratrol is a natural antioxidant with diverse biological effects, including the modulation of immune function, anti-inflammation, and cancer chemoprevention. In this study, we proved that S. aureus -upregulated vascular cell adhesion molecule-1 (VCAM-1) expression in human lung epithelial cells (HPAEpiCs) was inhibited by resveratrol...
October 7, 2018: International Journal of Molecular Sciences
Dong Yeong Min, Euitaek Jung, Juhwan Kim, Young Han Lee, Soon Young Shin
Leptin, an adipokine regulating energy metabolism, appears to be associated with breast cancer progression. Insulin-like growth factor-1 (IGF-1) mediates the pathogenesis of breast cancer. The regulation of IGF-1 expression by leptin in breast cancer cells is unclear. Here, we found that leptin upregulates IGF-1 expression at the transcriptional level in breast cancer cells. Activating protein-1 (AP-1)-binding element within the proximal region of IGF-1 was necessary for leptin-induced IGF-1 promoter activation...
October 8, 2018: BMB Reports
Yujia Bai, Yunyao Jiang, Tingwu Liu, Fu Li, Jianmei Zhang, Yanyan Luo, Liang Zhang, Guilong Yan, Zuoshan Feng, Xueqin Li, Xinfeng Wang, Weicheng Hu
ETHNOPHARMACOLOGICAL RELEVANCE: A multi-herb Chinese medicinal formula consisting of a variety of medicinal and edible materials has long been consumed as a hot drink and immune enhancer for its efficiency to increase disease resistance in Xinjiang, China. However, no fundamental data has been collected associated with traditional consumption. The present work was designed to evaluate the immunostimulatory role of Xinjiang herbal tea (XMT-WE) in RAW 264.7 macrophages and cyclophosphamide (CTX)-induced immunosuppression mice model...
September 27, 2018: Journal of Ethnopharmacology
Li Zhao, Tao Zhang, Hao Geng, Zhi-Qi Liu, Zhao-Feng Liang, Zhi-Qiang Zhang, Jie Min, De-Xin Yu, Cai-Yun Zhong
Bladder cancer is the most common malignancy of the urinary tract. Long-term exposure to benzidine is one of the major causes of bladder cancer. However, the mechanism of benzidine-induced bladder cancer is not yet sufficiently characterized. Dysregulated cell proliferation serves a critical role in cancer initiation and development; whether benzidine promotes cell proliferation, and the role of MAPKs in this process, have not previously been investigated. The present study aimed to investigate the benzidine-induced modulation of intracellular mitogen-activated protein kinases (MAPKs) and activator protein-1 (AP-1) signaling cascades on cell proliferation in SV-40 immortalized human uroepithelial cells (SV-HUC-1)...
October 2018: Oncology Letters
Yao-Xin Gao, Hao-Heng Yu, Chuan He, Ming Li, Dan-Dan Guo, Jun-Jiang Lian, Hai-Jie Yang, Mian Wang, Lei Wang, Zhi-Wei Feng, Bin-Feng Cheng
Background and purpose: Fengshi Gutong capsule (FSGTC), a traditional herbal formula, has been used clinically in China for the treatment of arthritis. However, the mechanism underlying the therapeutic effects of FSGTC on osteoarthritis (OA) has not been elucidated. The present study investigated the function and mechanisms of FSGTC in rat OA model and interleukin (IL)-1β-stimulated synovial cells. Materials and methods: Rat OA model was established by intra-articular injection containing 4% papain. IL-1β-induced SW982 cells were used as an OA cell model...
2018: Frontiers in Pharmacology
Mingming Zhu, Cong Huang, Xiao Ma, Rui Wu, Weiwei Zhu, Xiaoting Li, Zhaofeng Liang, Feifei Deng, Jieshu Wu, Shanshan Geng, Chunfeng Xie, Caiyun Zhong
Prostate cancer is one of the most commonly diagnosed cancers in man. Studies have shown that phthalates may act as promoters in various types of cancer; however, the role of phthalates in prostate cancer has been rarely reported. The MAPK/AP-1 pathway is a vital regulator of cell proliferation in cancer. In this report we found that three typical phthalates, diethylhexyl phthalate (DEHP), Butyl benzyl phthalate (BBP) and Dibutyl phthalate (DBP), up-regulated cyclinD1 and PCNA, down-regulated P21, inducing proliferation of prostate cancer cells...
August 13, 2018: Environmental Toxicology and Pharmacology
Andrea González-Morales, Aintzane Zabaleta, Elizabeth Guruceaga, Marta M Alonso, Marc García-Moure, Joaquín Fernández-Irigoyen, Enrique Santamaría
Glioblastoma multiforme (GBM) is the most common and aggressive type of malignant glioma. Oncolytic adenoviruses are being modified to exploit the aberrant expression of proteins in tumor cells to increase the antiglioma efficacy. E1A mutant adenovirus Delta-24-RGD (DNX-2401) has shown a favorable toxicity profile and remarkable efficacy in a first-in-human phase I clinical trial. However, the comprehensive modulation of glioma metabolism in response to Delta-24-RGD infection is poorly understood. Integrating mass spectrometry based-quantitative proteomics, physical and functional interaction data, and biochemical approaches, we conducted a cell-wide study of cytosolic, nuclear, and secreted glioma proteomes throughout the early time course of Delta-24-RGD infection...
July 24, 2018: Oncotarget
Tong Wang, Xiaoxia Jin, Yingjun Liao, Qi Sun, Chaohong Luo, Gaoyang Wang, Fenghong Zhao, Yaping Jin
Subacute poisoning of 1,2-dichloroethane (1,2-DCE) has become a serious occupational problem in China, and brain edema is its main pathological consequence, but little is known about the underlying mechanisms. As the metabolite of 1,2-DCE, 2-chloroethanol (2-CE) is more reactive, and might play an important role in the toxic effects of 1,2-DCE. In our previous studies, we found that matrix metalloproteinases-9 (MMP-9) expression was enhanced in mouse brains upon treatment with 1,2-DCE, and in rat astrocytes exposed to 2-CE...
August 7, 2018: Cells
Xiaoxia Jin, Yingjun Liao, Xiaoqiong Tan, Jingjing Guo, Gaoyang Wang, Fenghong Zhao, Yaping Jin
Accumulated data have revealed that subacute poisoning of 1,2-dichloroethane (1,2-DCE), an industrial solvent used in some countries can cause encephalopathy, in which brain edema is the main pathological change. However, the underlying mechanisms are unclear. In the present study, we hypothesized that the p38 MAPK (p38) signaling pathway could be activated in 1,2-DCE-intoxicated mice, which in turn stimulates transcription factors, such as nuclear factor-κB (NF-κB) and activator protein-1 (AP-1), and then enhances the expression of proinflammatory factors, including matrix metalloproteinase-9 (MMP-9), finally leading to blood-brain barrier (BBB) disruption and brain edema formation...
August 3, 2018: Neurotoxicology
Showgy Y Ma'ayeh, Livia Knörr, Karin Sköld, Alexandra Granham, Brendan R E Ansell, Aaron R Jex, Staffan G Svärd
Giardia intestinalis is a parasitic protist that causes diarrhea in humans, affecting mainly children of the developing world, elderly and immunocompromised individuals. Humans are infected by two major Giardia assemblages (i.e. genetic subtypes), A and B, with the latter being the most common. So far, there is little information on molecular or cellular changes during infections with assemblage B. Here, we used RNA sequencing to study transcriptional changes in Caco-2 intestinal epithelial cells (IECs) co-incubated with assemblage B (GS isolate) trophozoites for 1...
2018: Frontiers in Cellular and Infection Microbiology
Nitin A Das, Andrea J Carpenter, Tadashi Yoshida, Senthil A Kumar, Sandeep Gautam, Ricardo Mostany, Reza Izadpanah, Ashok Kumar, Srinivas Mummidi, Ulrich Siebenlist, Bysani Chandrasekar
Persistent inflammation promotes development and progression of heart failure (HF). TWEAK (TNF-Related WEAK Inducer Of Apoptosis), a NF-κB- and/or AP-1-responsive proinflammatory cytokine that signals via TWEAK receptor (TWEAKR), is expressed at high levels in human and preclinical models of HF. Since the adapter molecule TRAF3IP2 (TRAF3 Interacting Protein 2) is an upstream regulator of various proinflammatory pathways, including those activated by NF-κB and AP-1, we hypothesized that targeting TRAF3IP2 inhibits TWEAK-induced proinflammatory and pro-fibrotic responses in vitro and in vivo...
August 2018: Journal of Molecular and Cellular Cardiology
Huatuo Zhu, Shujun Huang, Min Yue, Wenguo Chen, Chao Lu, Xinhe Lou, Chunxiao Li, Guodong Shan, Hongtan Chen, Xiaowei Xu, Guoqiang Xu, Lihua Chen
BACKGROUND: Ulcerative colitis (UC) is an idiopathic colonic mucosal disease, and its pathogenesis has not been fully understood. Up-frameshift protein 1 (UPF1) is a potential molecule for UC predicted by a computational approach. AIM: The present study aimed to validate the underlying mechanism of UPF1 in UC. METHODS: UPF1 expression was detected by qRT-PCR, western blotting, and immunohistochemistry in dextran sulfate sodium-induced colitis in mice...
October 2018: Digestive Diseases and Sciences
Ran Ma, Fang Yuan, Shaoxuan Wang, Yingping Liu, Tingting Fan, Fulai Wang
Acute pancreatitis (AP) is a common acute abdominal disease accompanied by systemic inflammatory response syndrome, and could even be complicated by multiple-organ damage. This study aimed to examine whether calycosin, an isoflavone isolated from Radix astragali with antioxidant and anti-inflammatory activity, could protect against AP induced by cerulein. To this end, Balb/C mice were injected with cerulein (50 μg/kg) to establish the animal model of AP. Calycosin (25 and 50 mg/kg, p.o.) was administered 1 h prior to the first cerulein injection...
September 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Li Li, Lin Cai, Linxin Zheng, Yujie Hu, Weifeng Yuan, Zhenhui Guo, Weifeng Li
Pulmonary fibrosis (PF) is a life-threatening interstitial lung disease. In this study, we tried to reveal the model of action between high-mobility group box 1 (HMGB1) and α -smooth muscle actin ( α -SMA) and the protective role of gefitinib in pulmonary fibrosis induced by the administration of bleomycin aerosol in mice. For the mechanism study, lung tissues were harvested two weeks after modeling to detect the coexpression of HMGB1 and α -SMA by immunohistochemistry and immunofluorescence staining. Protein-DNA interactions were analyzed using a pulldown assay to study the relationship between HMGB1 and α -SMA...
2018: Oxidative Medicine and Cellular Longevity
Jing Wang, Fang He, Lingqiang Chen, Qin Li, Song Jin, Hongmei Zheng, Jun Lin, Hong Zhang, Sha Ma, Jian Mei, Juan Yu
OBJECTIVE: To explore the molecular mechanism of Res in regulation of pulmonary fibrosis (PF). METHODS: Rats were injected with bleomycin (BLM) to establish a PF model and treated with resveratrol (Res) and/or miR-21 agomir. After 14 days, lung tissues were collected for Hematoxylin-eosin and Masson's staining, and real-time quantitative polymerase chain reaction and Western blot were performed to detect fibrosis-related protein expression and the activation of the TGF-β1/Smad pathway...
September 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Yun Yao, Kehai Liu, Yueliang Zhao, Xiaoqian Hu, Mingfu Wang
Pterostilbene (Pte) and 4′-Methoxyresveratrol (4MR) are methylated derivatives of resveratrol. We investigated the anti-inflammatory effect of Pte and 4MR in lipopolysaccharide (LPS)-stimulated RAW264.7 murine macrophages. Both Pte and 4MR significantly reduced LPS-induced nitric oxide release by inhibiting the inducible nitric oxide synthase mRNA expression. Moreover, both of them inhibited LPS-induced mRNA expression of inflammatory cytokines including monocyte chemoattractant protein (MCP)-1, interleukin (IL)-6 and IL-1β, and tumor necrosis factor α (TNF-α), and attenuated LPS-induced nuclear factor-κB (NF-κB) activation by decreasing p65 phosphorylation...
May 11, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Hyowon Choi, Weera Chaiyamongkol, Alexandra C Doolittle, Zariel I Johnson, Shilpa S Gogate, Zachary R Schoepflin, Irving M Shapiro, Makarand V Risbud
The nucleus pulposus (NP) of intervertebral discs experiences dynamic changes in tissue osmolarity because of diurnal loading of the spine. TonEBP/NFAT5 is a transcription factor that is critical in osmoregulation as well as survival of NP cells in the hyperosmotic milieu. The goal of this study was to investigate whether cyclooxygenase-2 (COX-2) expression is osmoresponsive and dependent on TonEBP, and whether it serves an osmoprotective role. NP cells up-regulated COX-2 expression in hyperosmotic media. The induction of COX-2 depended on elevation of intracellular calcium levels and p38 MAPK pathway, but independent of calcineurin signaling as well as MEK/ERK and JNK pathways...
June 8, 2018: Journal of Biological Chemistry
Miaomiao Yuan, Wei Meng, Wenzhen Liao, Sen Lian
Andrographis paniculata Nees is used as a functional food in Japan, Korea, India, and China. Andrographolide, a naturally occurring phytochemical identified in Andrographis paniculata, has been discovered to present anti-inflammatory and anticancer activities. Highly expressed interleukin (IL-8) has been detected in colorectal cancer and is implicated in angiogenesis. However, the effect and molecular mechanisms of IL-8 expression by andrographolide remain obscure in human colorectal cancer cells. The present study was aimed to investigate the effects of andrographolide on TNF-α-induced IL-8 expression and its underlying mechanisms...
May 23, 2018: Journal of Agricultural and Food Chemistry
Mrinalini Singh, Seema Yadav, Meetul Kumar, Shweta Saxena, Deepika Saraswat, Anju Bansal, Shashi B Singh
This study reports the role of MAPKs (JNK, ERK, and p38), and activator protein-1 (AP-1) transcription factor in the hypobaric hypoxia induced change in lung tissue. Healthy male Sprague-Dawley rats were exposed to hypobaric hypoxia for 6, 12, 24, 48, 72, and 120 hr. Hypoxia resulted in significant increase in reactive oxygen species (ROS), vascular endothelial growth factor (VEGF) and decreased nitric oxide (NO), these act as signaling molecules for activation of MAPK and also contribute in development of vascular leakage (an indicator of pulmonary edema) as confirmed by histological studies...
October 2018: Journal of Cellular Physiology
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