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AP-1 keratinocyte

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https://www.readbyqxmd.com/read/29789551/epidermal-glucocorticoid-and-mineralocorticoid-receptors-act-cooperatively-to-regulate-epidermal-development-and-counteract-skin-inflammation
#1
Judit Bigas, Lisa M Sevilla, Elena Carceller, Julia Boix, Paloma Pérez
Endogenous and synthetic glucocorticoids (GCs) regulate epidermal development and combat skin inflammatory diseases. GC actions can be mediated through the GC receptor (GR) and/or the mineralocorticoid receptor (MR), highly homologous ligand-activated transcription factors. While the role of GR as a potent anti-inflammatory mediator is well known, that of MR is not as clear, nor is whether these receptors cooperate or antagonize each other in the epidermis. To address this, we generated mice with epidermal-specific loss of both receptors (double knockout, DKO), and analyzed the phenotypical and functional consequences relative to single KOs or controls (CO)...
May 22, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29748977/paeonol-extracted-from-paeonia-suffruticosa-andr-ameliorated-uvb-induced-skin-photoaging-via-dld-nrf2-are-and-mapk-ap-1-pathway
#2
ZhengWang Sun, Juan Du, Eunson Hwang, Tae-Hoo Yi
Paeonia suffruticosa Andr. (PS) has been used in traditional Chinese medicine for a long time. However, there are no studies that investigate the preventive effects of PS on ultraviolet B (UVB)-induced photoaging. In this study, paeonol (PA) was detected the main compound in PS root. In vitro, PS and PA significantly inhibited UVB-induced phosphorylation of mitogen-activated protein kinase and activator protein 1 in keratinocytes, which consequently led to degradation of procollagen type I. On the other hand, PS and PA increased NAD(P)H:quinone oxidoreductase 1 and heme oxygenase-1 expression, confirmed by greater nuclear accumulation of nuclear factor E2-releated factor 2 (Nrf2)...
May 10, 2018: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/29437671/pharmacological-tlr4-antagonism-using-topical-resatorvid-blocks-solar-uv-induced-skin-tumorigenesis-in-skh-1-mice
#3
Karen Blohm-Mangone, Nichole B Burkett, Shekha Tahsin, Paul B Myrdal, Alhassan Aodah, Brenda Ho, Jaroslav Janda, Michelle McComas, Kathylynn Saboda, Denise J Roe, Zigang Dong, Ann M Bode, Emanuel F Petricoin, Valerie S Calvert, Clara Curiel-Lewandrowski, David S Alberts, Georg T Wondrak, Sally E Dickinson
An urgent need exists for the development of more efficacious molecular strategies targeting nonmelanoma skin cancer (NMSC), the most common malignancy worldwide. Inflammatory signaling downstream of Toll-like receptor 4 (TLR4) has been implicated in several forms of tumorigenesis, yet its role in solar UV-induced skin carcinogenesis remains undefined. We have previously shown in keratinocyte cell culture and SKH-1 mouse epidermis that topical application of the specific TLR4 antagonist resatorvid (TAK-242) blocks acute UV-induced AP-1 and NF-κB signaling, associated with downregulation of inflammatory mediators and MAP kinase phosphorylation...
May 2018: Cancer Prevention Research
https://www.readbyqxmd.com/read/29341262/reversine-inhibits-mmp-1-and-3-expressions-by-suppressing-of-ros-mapk-ap-1-activation-in-uv-stimulated-human-keratinocytes-and-dermal-fibroblasts
#4
Jeong-Mi Kim, Sun Young Kim, Eun-Mi Noh, Hyun-Kyung Song, Guem-San Lee, Kang-Beom Kwon, Young-Rae Lee
UVB has been shown to stimulate the generation of ROS, which subsequently results in activation of various intracellular signaling pathways and transcription factors (AP-1, NF-κB), These transcription factors are regulated by MAPKs, which increase cytokine and MMP expression. We examined the preventive effects of reversine on MMP-1 and -3 expressions in NHEKs and NHDFs exposed to UVB irradiation. Also, we confirmed that reversine decreased pro-inflammatory cytokine expression in NHEKs. The mechanism underlying the MMP-inhibitory effects of reversine occurred via the suppression of UVB-induced ROS generation and MAPK/AP-1 activation...
January 16, 2018: Experimental Dermatology
https://www.readbyqxmd.com/read/29335070/cell-proliferation-and-migration-mechanism-of-caffeoylserotonin-and-serotonin-via-serotonin-2b-receptor-in-human-keratinocyte-hacat-cells
#5
Hye-Eun Kim, Hyejoung Cho, Atsushi Ishihara, Byungkuk Kim, Okjoon Kim
Caffeoylserotonin (CaS), one derivative of serotonin (5-HT), is a secondary metabolite produced in pepper fruits with strong antioxidant activities. In this study, we investigated the effect of CaS on proliferation and migration of human keratinocyte HaCaT cells compared to that of 5-HT. CaS enhanced keratinocyte proliferation even under serum deficient condition. This effect of CaS was mediated by serotonin 2B receptor (5-HT2BR) related to the cell proliferation effect of 5-HT. We also confirmed that both CaS and 5-HT induced G1 progression via 5-HT2BR/ERK pathway in HaCaT cells...
April 2018: BMB Reports
https://www.readbyqxmd.com/read/29273799/rna-seq-identifies-a-diminished-differentiation-gene-signature-in-primary-monolayer-keratinocytes-grown-from-lesional-and-uninvolved-psoriatic-skin
#6
William R Swindell, Mrinal K Sarkar, Yun Liang, Xianying Xing, Jaymie Baliwag, James T Elder, Andrew Johnston, Nicole L Ward, Johann E Gudjonsson
Keratinocyte (KC) hyper-proliferation and epidermal thickening are characteristic features of psoriasis lesions, but the specific contributions of KCs to plaque formation are not fully understood. This study used RNA-seq to investigate the transcriptome of primary monolayer KC cultures grown from lesional (PP) and non-lesional (PN) biopsies of psoriasis patients and control subjects (NN). Whole skin biopsies from the same subjects were evaluated concurrently. RNA-seq analysis of whole skin identified a larger number of psoriasis-increased differentially expressed genes (DEGs), but analysis of KC cultures identified more PP- and PN-decreased DEGs...
December 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29145717/ctrp9-regulates-growth-differentiation-and-apoptosis-in-human-keratinocytes-through-tgf%C3%AE-1-p38-dependent-pathway
#7
Tae Woo Jung, Hyung Sub Park, Geum Hee Choi, Daehwan Kim, Taeseung Lee
Impairment of wound healing is a common problem in individuals with diabetes. Adiponectin, an adipocyte-derived cytokine, has many beneficial effects on metabolic disorders such as diabetes, obesity, hypertension, and dyslipidemia. C1q/TNF-Related Protein 9 (CTRP9), the closest paralog of adiponectin, has been reported to have beneficial effects on wound healing. In the current study, we demonstrate that CTRP9 regulates growth, differentiation, and apoptosis of HaCaT human keratinocytes. We found that CTRP9 augmented expression of transforming growth factor beta 1 (TGFβ1) by transcription factor activator protein 1 (AP-1) binding activity and phosphorylation of p38 in a dose-dependent manner...
December 31, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28953906/analysis-of-chromatin-accessibility-in-human-epidermis-identifies-putative-barrier-dysfunction-sensing-enhancers
#8
Julie M Lander, Dorothy M Supp, Hua He, Lisa J Martin, Xiaoting Chen, Matthew T Weirauch, Steven T Boyce, Raphael Kopan
To identify putative gene regulatory regions that respond to epidermal injury, we mapped chromatin dynamics in a stratified human epidermis during barrier maturation and disruption. Engineered skin substitutes (ESS) cultured at the air-liquid interface were used as a model of developing human epidermis with incomplete barrier formation. The epidermal barrier stabilized following engraftment onto immunocompromised mice, and was compromised again upon injury. Modified formaldehyde-assisted isolation of regulatory elements (FAIRE) was used to identify accessible genomic regions characteristic of monolayer keratinocytes, ESS in vitro, grafted ESS, and tape-stripped ESS graft...
2017: PloS One
https://www.readbyqxmd.com/read/28948464/enhancement-of-protective-effects-of-radix-scutellariae-on-uvb-induced-photo-damage-in-human-hacat-keratinocytes
#9
Yu-Shuai Wang, Jin-Gyeong Cho, Eun-Son Hwang, Jung-Eun Yang, Wei Gao, Min-Zhe Fang, Sheng-Dao Zheng, Tae-Hoo Yi
Radix Scutellariae (RS) has long been used in the treatment of inflammatory and allergic diseases. Its main flavonoids, baicalin (BG) and wogonoside (WG), can be hydrolyzed into their corresponding aglycones, baicalein (B) and wogonin (W). In this study, we developed a safe and effective method of transforming these glycosides using Peclyve PR. The transformation rate of BG and WG reached 98.5 and 98.1%, respectively, with 10% enzyme at 40 °C for 60 h. Furthermore, we compared the anti-photoaging activity of RS before and after enzyme treatment, as well as their respective main components, in UVB-irradiated HaCaT cells...
April 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28936777/phytosphingosine-enhances-moisture-level-in-human-skin-barrier-through-stimulation-of-the-filaggrin-biosynthesis-and-degradation-leading-to-nmf-formation
#10
Hyun Kyung Choi, Young Hoon Cho, Eun Ok Lee, Jin Wook Kim, Chang Seo Park
Phytosphingosine (PHS) is a sphingoid that is a key component of phytoceramides NP, AP and EOP. PHS has been known to have anti-inflammation and antimicrobial activities and to stimulate epidermal differentiation. In addition, it is reported that PHS treatment notably increased phytoceramide content in keratinocytes. In this study, we tried to investigate whether PHS has any effect on the maturation of corneocytes such as formation of cornified envelope and natural moisturizing factor (NMF) that is also an essential event during the formation of skin barrier, stratum corneum...
December 2017: Archives of Dermatological Research
https://www.readbyqxmd.com/read/28602508/cyr61-ccn1-induces-ccl20-production-by-keratinocyte-via-activating-p38-and-jnk-ap-1-pathway-in-psoriasis
#11
Huidan Li, Haichuan Li, Rongfen Huo, Pinru Wu, Zhengyu Shen, Hui Xu, Baihua Shen, Ningli Li
BACKGROUND: Psoriasis is a common chronic skin disease characterized by epidermal hyperplasia and inflammation. Cysteine-rich angiogenic inducer 61 (Cyr61/CCN1) has recently been implicated in psoriasis pathogenesis by promoting keratinocyte activation. However, the mechanisms by which CCN1 enhances cutaneous inflammation are not fully understood. OBJECTIVE: In this study, we investigated the role of CCN1 on the expression of CCL20 in human keratinocyte. METHODS AND RESULTS: By double-label immunofluorescence staining, we first identified that the expression of CCN1 colocalized well with CCL20 production in the epidermis of psoriasis skin lesion...
October 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28526300/embryonic-ap1-transcription-factor-deficiency-causes-a-collodion-baby-like-phenotype
#12
Christina A Young, Richard L Eckert, Gautam Adhikary, Debra Crumrine, Peter M Elias, Miroslav Blumenberg, Ellen A Rorke
AP1 transcription factors are important controllers of gene expression in the epidermis, and altered AP1 factor function can perturb keratinocyte proliferation and differentiation. However, our understanding of how AP1 signaling changes may underlie or exacerbate skin disease is limited. We have shown that inhibiting AP1 factor function in suprabasal adult epidermis leads to reduced filaggrin levels and to a phenotype that resembles the genetic disorder ichthyosis vulgaris. We now show that inhibiting AP1 factor function during development in embryonic epidermis produces marked phenotypic changes including reduced filaggrin mRNA and protein levels, compromised barrier function, marked ultrastructural change, and enhanced dehydration susceptibility that resembles the phenotype observed in the flaky tail mouse, a model for ichthyosis vulgaris...
September 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28465707/extract-from-periostracum-cicadae-inhibits-oxidative-stress-and-inflammation-induced-by-ultraviolet-b-irradiation-on-hacat-keratinocytes
#13
Tsong-Min Chang, Jen-Horng Tsen, Hsuan Yen, Ting-Ya Yang, Huey-Chun Huang
Periostracum cicadae is widely used for the treatment of skin diseases such as eczema, pruritus, and itching. The current study sought to evaluate the effect of P. cicadae extract on ultraviolet B (UVB) irradiation and identify the mechanisms involved. Photodamage-protective activity of P. cicadae extracts against oxidative challenge was screened using HaCaT keratinocytes. P. cicadae extracts did not affect cell viability but decreased reactive oxygen species (ROS) production. The extract attenuates the expression of interleukin-6 (IL-6), matrix metalloproteinase-2 (MMP-2), and MMP-9 in UVB-treated HaCaT cells...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28379603/the-ginsenoside-derivative-20-s-protopanaxadiol-inhibits-solar-ultraviolet-light-induced-matrix-metalloproteinase-1-expression
#14
Seungmin Han, Tae-Gyu Lim, Jong-Eun Kim, Hee Yang, Deok-Kun Oh, Jung Han Yoon Park, Hee Jung Kim, Young Kyoung Rhee, Ki Won Lee
Ginsenosides are major pharmacologically active compounds present in ginseng (Panax ginseng). Among the ginsenosides, 20-O-β-D-glucopyranosyl-20(S)-protopanaxadiol (GPPD) and ginsenoside Rb1 (Rb1) have previously been reported to exhibit anti-wrinkle effects. In this study, 20(S)-protopanaxadiol (20(S)-PPD), an aglycone derivative of the Rb1 metabolite was investigated for its anti-wrinkle benefit and compared to GPPD and Rb1. The anti-wrinkle effect of 20(S)-PPD during solar UV light was investigated using a human skin equivalent model and human keratinocytes...
November 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28131816/traf1-is-critical-for-dmba-solar-uvr-induced-skin-carcinogenesis
#15
Hiroyuki Yamamoto, Joohyun Ryu, Eli Min, Naomi Oi, Ruihua Bai, Tatyana A Zykova, Dong Hoon Yu, Kenji Moriyama, Ann M Bode, Zigang Dong
TRAF1 is a member of the TRAF protein family, which regulates the canonical and noncanonical NF-κB signaling cascades. Although aberrant TRAF1 expression in tumors has been reported, the role of TRAF1 remains elusive. Here, we report that TRAF1 is required for solar UV-induced skin carcinogenesis. Immunohistochemical analysis showed that TRAF1 expression is up-regulated in human actinic keratosis and squamous cell carcinoma. In vivo studies indicated that TRAF1 expression levels in mouse skin are induced by short-term solar UV irradiation, and a long-term skin carcinogenesis study showed that deletion of TRAF1 in mice results in a significant inhibition of skin tumor formation...
June 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28024924/recognition-of-propionibacterium-acnes-by-human-tlr2-heterodimers
#16
Qi Su, Maria Grabowski, Günther Weindl
Propionibacterium acnes has been considered as a crucial contributor to the pathogenesis of acne vulgaris. The interaction between P. acnes and the host is mainly mediated by Toll like receptor (TLR) 2 recognition. TLR2 homodimers recognize P. acnes in mice, but here we describe the prerequisite of TLR2/1 and TLR2/6 heterodimers in human cells for P. acnes recognition. P. acnes-induced NF-κB and AP-1activation observed in HEK hTLR2-transfected but not control cells confirmed the specificity of TLR2 recognition...
February 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28011874/activation-of-nrf2-reduces-uva-mediated-mmp-1-upregulation-via-mapk-ap-1-signaling-cascades-the-photoprotective-effects-of-sulforaphane-and-hispidulin
#17
Anyamanee Chaiprasongsuk, Jinaphat Lohakul, Kitipong Soontrapa, Somponnat Sampattavanich, Pravit Akarasereenont, Uraiwan Panich
UVA irradiation plays a role in premature aging of the skin through triggering oxidative stress-associated stimulation of matrix metalloproteinase-1 (MMP-1) responsible for collagen degradation, a hallmark of photoaged skin. Compounds that can activate nuclear factor E2-related factor 2 (Nrf2), a transcription factor regulating antioxidant gene expression, should therefore serve as effective antiphotoaging agents. We investigated whether genetic silencing of Nrf2 could relieve UVA-mediated MMP-1 upregulation via activation of mitogen-activated protein kinase (MAPK)/activator protein 1 (AP-1) signaling using human keratinocyte cell line (HaCaT)...
March 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/27859308/resatorvid-based-pharmacological-antagonism-of-cutaneous-tlr4-blocks-uv-induced-nf-%C3%AE%C2%BAb-and-ap-1-signaling-in-keratinocytes-and-mouse-skin
#18
Jaroslav Janda, Nichole B Burkett, Karen Blohm-Mangone, Vivian Huang, Clara Curiel-Lewandrowski, David S Alberts, Emanuel F Petricoin, Valerie S Calvert, Janine Einspahr, Zigang Dong, Ann M Bode, Georg T Wondrak, Sally E Dickinson
Cutaneous exposure to solar ultraviolet (UV) radiation is a major causative factor in skin carcinogenesis, and improved molecular strategies for efficacious chemoprevention of nonmelanoma skin cancer (NMSC) are urgently needed. Toll-like receptor 4 (TLR4) signaling has been shown to drive skin inflammation, photoimmunosuppression, and chemical carcinogenesis. Here we have examined the feasibility of genetic and pharmacological antagonism targeting cutaneous TLR4 for the suppression of UV-induced NF-κB and AP-1 signaling in keratinocytes and mouse skin...
November 2016: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/27834922/bee-venom-inhibits-porphyromonas-gingivalis-lipopolysaccharides-induced-pro-inflammatory-cytokines-through-suppression-of-nf-%C3%AE%C2%BAb-and-ap-1-signaling-pathways
#19
Woon-Hae Kim, Hyun-Jin An, Jung-Yeon Kim, Mi-Gyeong Gwon, Hyemin Gu, Jae-Bok Park, Woo Jung Sung, Yong-Chul Kwon, Kyung-Duck Park, Sang Mi Han, Kwan-Kyu Park
Periodontitis is a chronic inflammatory disease that leads to destruction of tooth supporting tissues. Porphyromonas gingivalis (P. gingivalis), especially its lipopolysaccharides (LPS), is one of major pathogens that cause periodontitis. Bee venom (BV) has been widely used as a traditional medicine for various diseases. Previous studies have demonstrated the anti-inflammatory, anti-bacterial effects of BV. However, a direct role and cellular mechanism of BV on periodontitis-like human keratinocytes have not been explored...
November 10, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27705914/extrachromosomal-hpv-16-lcr-transcriptional-activation-by-hdaci-opposed-by-cellular-differentiation-and-dna-integration
#20
Ekaterina Dimitrova Bojilova, Christine Weyn, Marie-Hélène Antoine, Véronique Fontaine
Histone deacetylase inhibitors (HDACi) have been shown to render HPV-carrying cells susceptible to intrinsic and extrinsic apoptotic signals. As such, these epigenetic drugs have entered clinical trials in the effort to treat cervical cancer. Here, we studied the effect of common HDACi, with an emphasis on Trichostatin A (TSA), on the transcriptional activity of the HPV-16 Long Control Region (LCR) in order to better understand the impact of these agents in the context of the HPV life cycle and infection. HDACi strongly induced transcription of the firefly luciferase reporter gene under the control of the HPV-16 LCR in a variety of cell lines...
November 15, 2016: Oncotarget
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