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https://www.readbyqxmd.com/read/30091803/lgi3-promotes-human-keratinocyte-differentiation-via-the-akt-pathway
#1
In Wook Kim, Hyo-Soon Jeong, Nyoun Soo Kwon, Kwang Jin Baek, Hye-Young Yun, Dong-Seok Kim
Leucine-rich repeat LGI family member 3 (LGI3), a member of the LGI family, is a secreted protein that is expressed not only in the brain and adipose tissues, but also in various skin cells. We previously reported that LGI3 was secreted after exposure to ultraviolet B and promoted the migration of HaCaT human keratinocytes. In the present study, we investigated whether LGI3 influences the differentiation of keratinocytes. The results show that the expression of involucrin, a keratinocyte differentiation marker was reduced in tissue from LGI3 knockout mice...
August 9, 2018: Experimental Dermatology
https://www.readbyqxmd.com/read/30015902/spilanthol-inhibits-tnf%C3%A2-%C3%AE-%C3%A2-induced-icam%C3%A2-1-expression-and-pro%C3%A2-inflammatory-responses-by-inducing-heme-oxygenase%C3%A2-1-expression-and-suppressing-pjnk-in-hacat-keratinocytes
#2
Chun-Hsun Huang, Li-Chun Chang, Sindy Hu, Chien-Yu Hsiao, Shu-Ju Wu
Spilanthol has been reported to possess antioxidant, anti‑inflammatory, antimicrobial and antinociceptive properties. At present, the literature has reported the beneficial role of spilanthol on tumor necrosis factor‑α (TNF‑α)‑stimulated HaCaT cells. The present study investigated the effects of spilanthol on the expression of TNF‑α‑induced intercellular adhesion molecule 1 (ICAM‑1) and cyclooxygenase (COX)‑2 in the human keratinocyte cell line HaCaT. Cells were pretreated with various concentrations of spilanthol (10‑150 µM) followed by TNF‑α to induce inflammation...
September 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/30004816/gallotannin-improves-the-photoaged-related-proteins-by-extracellular-signal-regulated-kinases-c-jun-n-terminal-kinases-signaling-pathway-in-human-epidermal-keratinocyte-cells
#3
Gyoseon Goya M Ryeom, Weon Jeong Bang, Young Bu Kim, Go Eun Lee
Tannins are a type of polyphenols found in several fruits such as grapes and berries, and nuts such as aronias and acorns. Both hydrolyzable tannins and condensed tannins are referred to as tannins. Among the hydrolyzable tannins, gallotannin has a strong antioxidative property and is known to protect the skin by inhibiting the precursors of elastolytic enzymes. However, its mechanism of protection against ultraviolet B (UVB) damage in human fibroblasts and keratinocytes has not yet been elucidated. In this study, we investigate the antioxidant and antiaging effect of gallotannin on UVB-irradiated human cells by studying its effect on extracellular signal-regulated kinases/c-Jun N-terminal kinases (EKRs/JNKs) signaling related to cell growth and differentiation/stress apoptosis...
July 13, 2018: Journal of Medicinal Food
https://www.readbyqxmd.com/read/29914105/the-extract-of-d-dasycarpus-ameliorates-oxazolone-induced-skin-damage-in-mice-by-anti-inflammatory-and-antioxidant-mechanisms
#4
Tsong-Min Chang, Ting-Ya Yang, Yu-Lin Niu, Huey-Chun Huang
Dictamni dasycarpus is a type of Chinese medicine made from the root bark of D. dasycarpus . It has been reported to show a wide spectrum of biological and pharmacological effects, for example, it has been used widely for the treatment of rheumatism, nettle rash, itching, jaundice, chronic hepatitis and skin diseases. In the current study, D. dasycarpus extract was investigated for its antioxidant and anti-inflammatory effects, as well as its capability to alleviate oxazolone-induced skin damage in mice. The possible anti-inflammatory mechanism of D...
June 15, 2018: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/29882826/a-muscarinic-antagonist-reduces-airway-inflammation-and-bronchoconstriction-induced-by-ambient-particulate-matter-in-a-mouse-model-of-asthma
#5
Jun Kurai, Masanari Watanabe, Hiroyuki Sano, Kyoko Iwata, Degejirihu Hantan, Eiji Shimizu
Ambient particulate matter (PM) can increase airway inflammation and induce bronchoconstriction in asthma. This study aimed to investigate the effect of tiotropium bromide, a long-acting muscarinic antagonist, on airway inflammation and bronchoconstriction induced by ambient PM in a mouse model of asthma. We compared the effect of tiotropium bromide to that of fluticasone propionate and formoterol fumarate. BALB/c mice were sensitized to ovalbumin (OVA) via the airways and then administered tiotropium bromide, fluticasone propionate, or formoterol fumarate...
June 6, 2018: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29743348/humidity-regulated-clca2-protects-the-epidermis-from-hyperosmotic-stress
#6
Kristin Seltmann, Michael Meyer, Jitka Sulcova, Tobias Kockmann, Ulrike Wehkamp, Stephan Weidinger, Ulrich Auf dem Keller, Sabine Werner
Low environmental humidity aggravates symptoms of the inflammatory skin disease atopic dermatitis (AD). Using mice that develop AD-like signs, we show that an increase in environmental humidity rescues their cutaneous inflammation and associated epidermal abnormalities. Quantitative proteomics analysis of epidermal lysates of mice kept at low or high humidity identified humidity-regulated proteins, including chloride channel accessory 3A2 (CLCA3A2), a protein with previously unknown function in the skin. The epidermis of patients with AD, organotypic skin cultures under dry conditions, and cultured keratinocytes exposed to hyperosmotic stress showed up-regulation of the nonorthologous human homolog CLCA2...
May 9, 2018: Science Translational Medicine
https://www.readbyqxmd.com/read/29709911/sphingoid-base-upregulated-caspase-14-expression-involves-mapk
#7
Yukitoshi Nagahara, Kei Kawakami, Abudubari Sikandan, Daiki Yagi, Ryo Nishikawa, Takahisa Shinomiya
Sphingolipids are putative intracellular signal mediators in cell differentiation, growth inhibition, and apoptosis. Especially, sphingoid base-backbones of sphingolipids (sphingosine, sphinganine, and phytosphingosine) and their metabolites N-acyl-sphingoid bases (ceramides) are highly bioactive. In skin, one of the caspases, caspase-14, is expressed predominantly in cornifying epithelia, and caspase-14 plays an important role in keratinocyte differentiation. As ceramides were surrounding lipids in the keratinocytes and ceramides stimulate keratinocyte differentiation, we therefore examined the upregulation of caspase-14 by various sphingoid bases and ceramide...
2018: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/29678305/the-effect-of-cilostazol-a-phosphodiesterase-3-pde3-inhibitor-on-human-hair-growth-with-the-dual-promoting-mechanisms
#8
Hye-In Choi, Dong Young Kim, Soon-Jin Choi, Chang-Yup Shin, Sungjoo Tommy Hwang, Kyu Han Kim, Ohsang Kwon
BACKGROUND: Cilostazol, a phosphodiesterase 3 (PDE3) inhibitor, increases the intracellular level of cyclic adenosine monophosphate to cause vasodilation. Topical application of cilostazol is reported to improve local blood flow and enhance wound healing; however, its effect on human hair follicles is unknown. OBJECTIVE: The purpose of this study was to determine the effect of cilostazol on hair growth. METHODS: We investigated the expression of PDE3 in human dermal papilla cells (DPCs), outer root sheath cells (ORSCs), and hair follicles...
July 2018: Journal of Dermatological Science
https://www.readbyqxmd.com/read/29621312/reactive-oxygen-species-and-nitric-oxide-signaling-in-bystander-cells
#9
Kishore Kumar Jella, Roisin Moriarty, Brendan McClean, Hugh J Byrne, Fiona M Lyng
It is now well accepted that radiation induced bystander effects can occur in cells exposed to media from irradiated cells. The aim of this study was to follow the bystander cells in real time following addition of media from irradiated cells and to determine the effect of inhibiting these signals. A human keratinocyte cell line, HaCaT cells, was irradiated (0.005, 0.05 and 0.5 Gy) with γ irradiation, conditioned medium was harvested after one hour and added to recipient bystander cells. Reactive oxygen species, nitric oxide, Glutathione levels, caspase activation, cytotoxicity and cell viability was measured after the addition of irradiated cell conditioned media to bystander cells...
2018: PloS One
https://www.readbyqxmd.com/read/29620265/ashwagandha-root-extract-exerts-anti%C3%A2-inflammatory-effects-in-hacat-cells-by-inhibiting-the-mapk-nf%C3%A2-%C3%AE%C2%BAb-pathways-and-by-regulating-cytokines
#10
Abudubari Sikandan, Takahisa Shinomiya, Yukitoshi Nagahara
A paste composed of the boiled leaves and roots of the Ashwagandha plant is used to cure ulcer and swelling in Ayurvedic medicine. However, the effects of the hot water extract of Ashwagandha roots (ASH‑WEX), which is also used in Ayurveda, on skin have not been fully elucidated. Therefore, the present study investigated the anti‑inflammatory activity of ASH‑WEX on skin, by using the human keratinocyte cell line HaCaT. The results indicated that ASH‑WEX significantly inhibited mRNA expression of inflammatory cytokines, including interleukin (IL)‑8, IL‑6, tumor necrosis factor (TNF‑α), IL‑1β and IL‑12, and promoted the mRNA expression of the anti‑inflammatory cytokine transforming growth factor (TGF)‑β1 in HaCaT cells...
July 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29458473/epidermidibacterium-keratini-gen-nov-sp-nov-a-member-of-the-family-sporichthyaceae-isolated-from-keratin-epidermis
#11
Dong-Geol Lee, Martha E Trujillo, Seunghyun Kang, Jin-Ju Nam, Yeon-Jun Kim
A novel actinobacterial strain, designated EPI-7T , was isolated on R2A agar from human skin (keratinocytes) and subjected to a taxonomic study using a polyphasic approach. Strain EPI-7T showed a Gram-positive reaction, was non-motile, non-spore-forming, and cells had a rod-shape. Colonies were round, convex and pale yellow. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel isolate formed a cluster with several uncultured bacterial clones and with cultured members of the genera Modestobacter and Sporichthya...
March 2018: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/29436626/effects-of-recombinant-human-parathyroid-hormone-1-34-on-cell-proliferation-chemokine-expression-and-the-hedgehog-pathway-in-keratinocytes
#12
Xiaolin Bu, Xinling Bi, Wuqing Wang, Yuling Shi, Qiang Hou, Jun Gu
Psoriasis is an autoimmune disease involving the excessive proliferation of keratinocytes mediated by T‑cells. Parathyroid hormone (PTH) has been identified as an essential factor in the treatment of psoriasis. In the present study, the mechanism underlying the effect of recombinant human parathyroid hormone (rhPTH) (1‑34) in keratinocytes was investigated. The effects of rhPTH (1‑34) on cell proliferation, cell cycle, and the secretion and expression of C‑X‑C motif chemokine 11 (CXCL11) and components of the Hedgehog signaling pathway were examined in HaCaT cells by MTT assay, flow cytometric analysis, ELISA and gene chip analysis...
April 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29395576/reduced-hmgb1-suppresses-poly-i-c-induced-inflammation-in-keratinocytes
#13
Hideki Mori, Masamoto Murakami, Teruko Tsuda, Kenji Kameda, Ryo Utsunomiya, Kana Masuda, Ken Shiraishi, Xiuju Dai, Mikiko Tohyama, Hiroki Nakaoka, Koji Sayama
BACKGROUND: High mobility group box 1 (HMGB1) is a nuclear protein that stabilizes DNA and facilitates gene transcription. Additionally, cell stress or death induces the release of HMGB1 outside the cell membrane, where HMGB1 functions as an alarmin, causing an inflammatory response in combination with other cytokines, damage-associated molecular patterns (DAMPs), and pathogen-associated molecular patterns (PAMPs). OBJECTIVE: To evaluate the effect of reduced-HMGB1 (previously termed chemoattractive-HMGB1) on polyinosine-polycytidylic acid [poly(I:C)]-induced inflammation in normal human keratinocytes (NHKs)...
May 2018: Journal of Dermatological Science
https://www.readbyqxmd.com/read/29317682/piperonylic-acid-stimulates-keratinocyte-growth-and-survival-by-activating-epidermal-growth-factor-receptor-egfr
#14
Dohyun Lee, Jinsun Lim, Kyung-Chul Woo, Kyong-Tai Kim
Epidermal growth factor (EGF) stimulates cell growth, proliferation, and survival. The biological benefits of EGF have been utilized in medical uses for improving wound healing as well as in today's skin cosmetics. EGF has been found in urine, saliva, milk, and plasma, but its efficient isolation remains a difficult task. With technical advances, recombinant protein purification technique has been used for EGF production. However, the recombinant EGF is still expensive and keeping it with stable activity is difficult to be used widely...
January 9, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29235124/silver-nanoparticles-increase-connexin43-mediated-gap-junctional-intercellular-communication-in-hacat-cells-through-activation-of-reactive-oxygen-species-and-mitogen-activated-protein-kinase-signal-pathway
#15
Yu Qin, Limin Han, Di Yang, Hongying Wei, Yue Liu, Junhui Xu, Herman Autrup, Furong Deng, Xinbiao Guo
Silver nanoparticles (AgNPs) are widely used in health and consumer products that routinely contact skin. However, the biological effects and possible mechanisms of AgNPs on skin remain unclear. Gap junctional intercellular communication (GJIC) plays a critical role in multicellular organisms to maintain tissue homeostasis. The aim of this study is to examine if non-coated AgNPs affect GJIC in human keratinocytes (HaCaT cells), and to identify the possible molecular mechanisms responsible for the effects. GJIC, connexin (Cx)43 protein and mRNA expression, and the effect of siRNA-mediated knockdown of Cx43 on GJIC were assessed...
April 2018: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/29127427/amniotic-membrane-promotes-focal-adhesion-remodeling-to-stimulate-cell-migration
#16
Ángel Bernabé-García, Sergio Liarte, Jose M Moraleda, Gregorio Castellanos, Francisco J Nicolás
During wound healing, the migration of keratinocytes onto newly restored extracellular matrix aims to reestablish continuity of the epidermis. The application of amniotic membrane (AM) to chronic, deep traumatic, non-healing wounds has proven successful at stimulating re-epithelialization. When applied on epithelial cell cultures, AM activates extracellular signal-regulated kinases 1/2 (ERK1/2) and c-Jun N-terminal kinases 1/2 (JNK1/2), with the overexpression and phosphorylation of c-Jun along the wound edge...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29087009/high-calcium-enhances-the-expression-of-double-stranded-rna-sensors-and-antiviral-activity-in-epidermal-keratinocytes
#17
Yuriko Yamamura, Shin Morizane, Takenobu Yamamoto, Jun Wada, Keiji Iwatsuki
Double-stranded RNA (dsRNA) sensors including TLR3, MDA5 and RIG-I are expressed in epidermal keratinocytes and play an important immunological role by enhancing various innate and adaptive immune responses. Although the role of elevated extracellular calcium concentration in keratinocyte differentiation is well understood, the effect of high calcium on dsRNA sensors is not well studied. We investigated alterations in dsRNA sensor expression and antiviral activity induced by a high extracellular concentration of calcium in epidermal keratinocytes...
February 2018: Experimental Dermatology
https://www.readbyqxmd.com/read/29072680/transglutaminase-2-mediates-uv-induced-skin-inflammation-by-enhancing-inflammatory-cytokine-production
#18
Seok-Jin Lee, Ki Baek Lee, Young Hoon Son, Jiwoong Shin, Jin-Haeng Lee, Hyo-Jun Kim, Ah-Young Hong, Hee Won Bae, Mee-Ae Kwon, Won Jong Lee, Jin-Hee Kim, Dong Hun Lee, Eui Man Jeong, In-Gyu Kim
UV irradiation elicits acute inflammation in the skin by increasing proinflammatory cytokine production in keratinocytes. However, the downstream protein target(s) that link UV radiation to the activation of signaling pathways responsible for cytokine expression have not been fully elucidated. In this study, we report a novel role of transglutaminase 2 (TG2), a member of the TG enzyme family whose activities are critical for cornified envelope formation, in mediating UV-induced inflammation. Our results showed that TG2-deficient mice exhibited reduced inflammatory responses to UV irradiation, including reduced erythema, edema, dilation of blood vessels, inflammatory cell infiltration, and levels of inflammatory cytokines...
October 26, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28975372/t-buooh-induces-ferroptosis-in-human-and-murine-cell-lines
#19
Christine Wenz, Dagmar Faust, Berenike Linz, Christian Turmann, Teodora Nikolova, John Bertin, Peter Gough, Peter Wipf, Anna Sophia Schröder, Stefan Krautwald, Cornelia Dietrich
Reactive oxygen species (ROS)-induced apoptosis has been extensively studied. Increasing evidence suggests that ROS, for instance, induced by hydrogen peroxide (H2 O2 ), might also trigger regulated necrotic cell death pathways. Almost nothing is known about the cell death pathways triggered by tertiary-butyl hydroperoxide (t-BuOOH), a widely used inducer of oxidative stress. The lipid peroxidation products induced by t-BuOOH are involved in the pathophysiology of many diseases, such as cancer, cardiovascular diseases, or diabetes...
February 2018: Archives of Toxicology
https://www.readbyqxmd.com/read/28842325/c-terminal-processing-of-collagen-xvii-induces-neoepitopes-for-linear-iga-dermatosis-autoantibodies
#20
Ellen Toyonaga, Wataru Nishie, Kentaro Izumi, Ken Natsuga, Hideyuki Ujiie, Hiroaki Iwata, Jun Yamagami, Yoshiaki Hirako, Daisuke Sawamura, Wataru Fujimoto, Hiroshi Shimizu
Transmembrane collagen XVII (COL17) is a hemidesmosomal component of basal keratinocytes that can be targeted by autoantibodies in autoimmune blistering disorders, including linear IgA dermatosis (LAD). COL17 can be physiologically cleaved within the juxtamembranous extracellular NC16A domain, and LAD autoantibodies preferentially react with the processed ectodomains, indicating that the processing induces neoepitopes. However, the details of how neoepitopes develop have not been elucidated. In this study, we show that C-terminal processing of COL17 also plays a role in inducing neoepitopes for LAD autoantibodies...
December 2017: Journal of Investigative Dermatology
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