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Shafiya Imtiaz Rafiqi, Rajat Garg, Reena K K, Hira Ram, Mithilesh Singh, P S Banerjee
Refractile body protein, SO7, is a highly immunogenic protein which is essentially involved in the early development of Eimeria species infecting the domestic chicken. In the present study, the immune response and protective efficacy of recombinant Eimeria tenella SO7 (rEtSO7) protein was assessed in broiler chickens following homologous oocyst challenge. Broiler chicks were subcutaneously immunized with rEtSO7 antigen adjuvanted with Montanide ISA 71 VG on 7 and 21 days of age and protective efficacy of vaccination was evaluated in terms of body weight gain, lesion score and reduction in oocyst output...
July 15, 2018: Veterinary Parasitology
Hui-Rong Xi, Fang-Fang Yang, Yu-Hai Gao, Hui-Ping Ma, Ke-Ming Chen
OBJECTIVE: To compare effects of resveratrol, puerarin and the compounds on peak bone mass in rats. METHODS: Forty SPF Wistar rats weighed 109.45 g to 119.44 g with an average of 115.87 g were selected. After 3 days' adaption, rats were divided into control group (the same volume of distilled water per day), puerarin group(15.4 mg/kg puerarin daily), resveratrol group (8.4 mg/kg resveratrol daily), compound drug group (daily dose of 8.4 resveratrol added 15.4 mg/kg of puerarin) and 10 in each group...
July 25, 2018: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
F M M Meijer, P Kies, M R M Jongbloed, S E van Wijngaarden, C A Swenne, S Man, M J Schalij, J K de Vries-Bouwstra, H W Vliegen
BACKGROUND: Patients with systemic sclerosis (SSc) are at risk for developing pulmonary hypertension (PH) which is a major cause of death in this population. Echocardiographic (TTE) derived pulmonary arterial pressure (PAP) can be unreliable for the early detection of PH. Previous studies demonstrate that the ECG derived ventricular gradient optimized for right ventricular pressure overload (VG-RVPO) can detect PH in a heterogeneous population suspected of PH. The aim of this study is to assess the use of the VG-RVPO as a screening and monitoring instrument of early PH in SSc patients...
July 24, 2018: International Journal of Cardiology
Constantinos Glynos, Sofia-Iris Bibli, Paraskevi Katsaounou, Athanasia Pavlidou, Christina Magkou, Vassiliki Karavana, Stavros Topouzis, Ioannis Kalomenidis, Spyros G Zakynthinos, Andreas Papapetropoulos
Electronic cigarettes (e-cig) are advertised as a less harmful nicotine delivery system or as a new smoking cessation tool. We aimed to assess the in vivo effects of e-cigarette vapor in the lung and to compare them to those of cigarette smoke (CS). We exposed C57BL/6 mice for either 3 days or 4 weeks to ambient air, CS or e-cig vapor containing: i) propylene glycol/vegetable glycerol (1:1; PG:VG-Sol), ii) PG:VG with nicotine (G:VG-N), or iii) PG:VG with nicotine and flavor (PG:VG- N+F) and determined oxidative stress, inflammation and pulmonary mechanics...
August 9, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
Marie Reine Haddad, Eun-Young Choi, Patricia M Zerfas, Ling Yi, Diego Martinelli, Patricia Sullivan, David S Goldstein, Jose A Centeno, Lauren R Brinster, Martina Ralle, Stephen G Kaler
Menkes disease is a lethal neurodegenerative disorder of copper metabolism caused by mutations in an evolutionarily conserved copper transporter, ATP7A. Based on our prior clinical and animal studies, we seek to develop a therapeutic approach suitable for application in affected human subjects, using the mottled-brindled ( mo-br ) mouse model that closely mimics the Menkes disease biochemical and clinical phenotypes. Here, we evaluate the efficacy of low-, intermediate-, and high-dose recombinant adeno-associated virus serotype 9 (rAAV9)-ATP7A delivered to the cerebrospinal fluid (CSF), in combination with subcutaneous administration of clinical-grade copper histidinate (sc CuHis, IND #34,166)...
September 21, 2018: Molecular Therapy. Methods & Clinical Development
Alessandra Ricciardi, Nicholas H Zelt, Kittipos Visitsunthorn, John P Dalton, Momar Ndao
A vaccine against schistosomiasis would contribute to a long-lasting decrease in disease spectrum and transmission. Our previous protection studies in mice using Schistosoma mansoni Cathepsin B (Sm-Cathepsin B) resulted in 59 and 60% worm burden reduction with CpG oligodeoxynucleotides and Montanide ISA720 VG as adjuvants, respectively. While both formulations resulted in significant protection in a mouse model of schistosomiasis, the elicited immune responses differed. Therefore, in this study, we aimed to decipher the mechanisms involved in Sm-Cathepsin B vaccine-mediated protection...
2018: Frontiers in Immunology
Fernanda C Lanza, Mariana M Reimberg, Raphael Ritti-Dias, Rebeca S Scalco, Gustavo F Wandalsen, Dirceu Sole, Marco van Brussel, H J Hulzebos, Simone Dal Corso, Tim Takken
Background: Oxygen uptake (VO2 ) evaluations by cardiopulmonary exercise test is expensive and time-consuming. Estimating VO2 based on a field test would be an alternative. Objective: To develop and validate an equation to predict VO2peak based on the modified shuttle test (MST). Methods: Cross sectional study, with 97 children and adolescents with asthma. Participants were divided in two groups: the equation group (EG), to construct the equation model of VO2peak , and the cross-validation group (VG). Each subject performed the MST twice using a portable gas analyzer...
2018: Frontiers in Physiology
Selcuk Anli, Afsaneh Karimian-Tabrizi, Anton Moritz, Nadejda Monsefi
BACKGROUND: The lower patency rate of vein grafts (VG) in comparison to arterial grafts may be related to vein valves, which favor turbulences and thrombosis that lead to graft failure. The aim of this study was to determine the outcome of patients with valvulotomized VG after coronary artery bypass grafting (CABG) procedure.  Methods: From 2007 to 2014, 233 patients with a mean age of 67 ± 9 years had CABG or combined CABG and valve procedures. Valvulotomized saphenous VG and arterial grafts were used...
June 18, 2018: Heart Surgery Forum
Zhefu Wu, Lei Jiang, Zhuangzhuang Jiang, Bin Chen, Kai Liu, Qi Xuan, Yun Xiang
Passive indoor localization techniques can have many important applications. They are nonintrusive and do not require users carrying measuring devices. Therefore, indoor localization techniques are widely used in many critical areas, such as security, logistics, healthcare, etc. However, because of the unpredictable indoor environment dynamics, the existing nonintrusive indoor localization techniques can be quite inaccurate, which greatly limits their real-world applications. To address those problems, in this work, we develop a channel state information (CSI) based indoor localization technique...
August 3, 2018: Sensors
Dongjie Cai, Quanjiang Song, Cong Duan, Shenghua Wang, Jiufeng Wang, Yaohong Zhu
The aim of this study was to develop and test an optimal vaccination strategy against bovine viral diarrhea virus (BVDV) based on the E2 glycoprotein of the BJ1305 strain. To achieve higher E2-specific antibody titers and to broaden the cellular immune response, a plasmid encoding the E2 protein (pcDNA3.1-E2) was constructed and a purified recombinant E2 protein was generated. The E2 protein was emulsified in the adjuvant ISA 61 VG prior to administration. We immunized mice three times with pcDNA3.1-E2 or the recombinant E2 protein or primed twice with pcDNA3...
August 1, 2018: Vaccine
Yu-Rong Wang, Ru-Tao Hong, Yuan-Yuan Xie, Jian-Ming Xu
Melatonin has been reported to inhibit hepatic fibrosis and the mechanism may be correlated to its anti-oxidant effect. Nevertheless, the mechanism is not completely identified. This study was conducted to investigate the effects of melatonin on TGF-β1/Smad signaling pathway in liver fibrosis in rats. The liver fibrosis model was made by the subcutaneous injection of CCl4 . The liver pathology changes were detected using hematoxylin and eosin (H&E) staining and Van Gieson (VG) staining. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were measured with an autoanalyzer...
April 2018: Current medical science
Rui Xu, Ying Jia, Deborah A Zygmunt, Megan L Cramer, Kelly E Crowe, Guohong Shao, Agatha E Maki, Haley N Guggenheim, Benjamin C Hood, Danielle A Griffin, Ellyn Peterson, Brad Bolon, John P Cheatham, Sharon L Cheatham, Kevin M Flanigan, Louise R Rodino-Klapac, Louis G Chicoine, Paul T Martin
Recombinant adeno-associated virus (rAAV)rh74.MCK. GALGT2 is a muscle-specific gene therapy that is being developed to treat forms of muscular dystrophy. Here we report on an isolated limb infusion technique in a non-human primate model, where hindlimb blood flow is transiently isolated using balloon catheters to concentrate vector in targeted leg muscles. A bilateral dose of 2.5 × 1013 vector genomes (vg)/kg/limb was sufficient to induce GALGT2-induced glycosylation in 10%-60% of skeletal myofibers in all leg muscles examined...
September 21, 2018: Molecular Therapy. Methods & Clinical Development
Sean M Crosson, Peter Dib, J Kennon Smith, Sergei Zolotukhin
Adeno-associated virus (AAV) is one of the most promising gene therapy vectors and is widely used as a gene delivery vehicle for basic research. As AAV continues to become the vector of choice, it is increasingly important for new researchers to have access to a simplified production and purification protocol for laboratory grade recombinant AAV. Here we report a detailed protocol for serotype independent production of AAV using a helper-free HEK293 cell system followed by iodixanol gradient purification, a method described earlier...
September 21, 2018: Molecular Therapy. Methods & Clinical Development
Aneesh Thakur, Athina Andrea, Heidi Mikkelsen, Joshua S Woodworth, Peter Andersen, Gregers Jungersen, Claus Aagaard
There is a need for the rational design of safe and effective vaccines to protect against chronic bacterial pathogens such as Mycobacterium tuberculosis and Mycobacterium avium subsp. paratuberculosis in a number of species. One of the main challenges for vaccine development is the lack of safe adjuvants that induce protective immune responses. Cationic Adjuvant Formulation 01 (CAF01)-an adjuvant based on trehalose dibehenate (TDB) and targeting the Mincle receptor-has entered human trials based on promising pre-clinical results in a number of species...
2018: PloS One
Wei Guo, Zhongxia Wu, Libin Yang, Zhaokui Cai, Lianfeng Zhao, Shutang Zhou
In addition to preventing insect metamorphosis, juvenile hormone (JH) is known to stimulate aspects of insect reproduction. However, the molecular mechanisms of JH action in insect reproduction remain largely unknown. By reanalyzing the transcriptomic data from adults and other developmental stages of the migratory locust Locusta migratoria, we identified a gene coding for Kazal-type protease inhibitor, previously named Greglin. Greglin is specifically expressed in adult females and most abundant in the fat body and ovaries...
July 31, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Jaana Karhu, Tero Ilmari Ala-Kokko, Tytti Vuorinen, Pasi Ohtonen, Ilkka Julkunen, Hannu Tapani Syrjälä
BACKGROUND: The serum cytokine levels among 45 mechanically ventilated, intensive care unit (ICU)-treated severe community-acquired pneumonia (SCAP) patients with known microbial etiology in three different etiology groups were assessed. METHODS: Blood samples for C-reactive protein (CRP), procalcitonin (PCT), interleukin (IL)-5, IL-6, IL-10, human interferon gamma induced protein (IP)-10, and TNF-α (tumor necrosis factor alpha) were collected at time points 0, 12, 24, 48, 72 and 96 h after study inclusion...
July 25, 2018: Cytokine
A P Wang, L Liu, L L Gu, C M Guo, S Wu, Q Feng, W L Xia, Z Wu, S Y Zhu
The avian adeno-associated virus (AAAV) has been proved to be an efficient gene transfer vector for human gene therapy and vaccine research. In this experiment, an AAAV-based vaccine was evaluated for the development of a vaccine against duck hepatitis a virus type 1 (DHAV-1). The major capsid VP1 gene was amplified and subcloned into pFBGFP containing the inverted terminal repeats of AAAV, and then the recombinant baculovirus rBac-VP1 was generated. The recombinant AAAV expressing the VP1 protein (rAAAV-VP1) was produced by co-infecting Sf9 cells with rBac-VP1 and the other 2 baculoviruses containing AAAV functional genes and structural genes respectively, and confirmed by electron microscopy, Western blotting and immunofluorescence assays...
July 21, 2018: Poultry Science
Janaina Gomes Maciel, Carolina Yoshi Campos Sugio, Giuliana de Campos Chaves, Andrea Lemos Falcão Procópio, Vanessa Migliorini Urban, Karin Hermana Neppelenbroek
STATEMENT OF PROBLEM: Specifications for determining acceptable limits of water sorption (WS) and solubility for interim denture resilient liners are lacking. PURPOSE: The purpose of this in vitro study was to evaluate the WS and solubility of interim resilient materials throughout their lifespans. MATERIAL AND METHODS: Specimens (n=10) of 7 tissue conditioners, Coe-Comfort (CC), Softone (ST), Rite-Line (RL), Dura Conditioner (DC), Hydrocast (HC), Dentusoft (DS), and Visco-gel (VG) and 2 interim resilient liners, Trusoft (TS) and Coe-Soft (CS), were submitted to desorption until weight stabilization...
July 20, 2018: Journal of Prosthetic Dentistry
Gyumin Lim, Kenneth David Kihm, Hong Goo Kim, Woorim Lee, Woomin Lee, Kyung Rok Pyun, Sosan Cheon, Phillip Lee, Jin Young Min, Seung Hwan Ko
The grain size of CVD (Chemical Vapor Deposition) graphene was controlled by changing the precursor gas flow rates, operation temperature, and chamber pressure. Graphene of average grain sizes of 4.1 µm, 2.2 µm, and 0.5 µm was synthesized in high quality and full coverage. The possibility to tailor the thermoelectric conversion characteristics of graphene has been exhibited by examining the grain size effect on the three elementary thermal and electrical properties of σ , S , and k . Electrical conductivity ( σ ) and Seebeck coefficients ( S ) were measured in a vacuum for supported graphene on SiO₂/Si FET (Field Effect Transistor) substrates so that the charge carrier density could be changed by applying a gate voltage (VG)...
July 22, 2018: Nanomaterials
Camila de Oliveira Ramos, Keila Karine Duarte Campos, Guilherme de Paula Costa, Sílvia Dantas Cangussú, André Talvani, Frank Silva Bezerra
Taurine is the major free amino acid found in mammalian cells and is known to be an antioxidant and membrane-stabilizing agent. This study aimed to evaluate the effects of taurine on oxidative stress and inflammatory response in the lungs of mice exposed to cigarette smoke. Fifty male C57BL/6 mice were divided into 5 groups: control group (CG), vehicle group (VG), taurine group (TG), cigarette smoke group (CSG), and cigarette smoke + taurine group (CSTG). For five consecutive days, CSG and CSTG were exposed to 4 cigarettes 3 times a day...
July 17, 2018: Regulatory Toxicology and Pharmacology: RTP
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