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Jerry K Y Chan, Irene Gil-Farina, Nuryanti Johana, Cecilia Rosales, Yi Wan Tan, Jessika Ceiler, Jenny Mcintosh, Bryan Ogden, Simon N Waddington, Manfred Schmidt, Arijit Biswas, Mahesh Choolani, Amit C Nathwani, Citra N Z Mattar
Adeno-associated viral vectors (AAVs) achieve stable therapeutic expression without long-term toxicity in adults with hemophilia. To avert irreversible complications in congenital disorders producing early pathogenesis, safety and efficacy of AAV-intrauterine gene transfer (IUGT) requires assessment. We therefore performed IUGT of AAV5 or -8 with liver-specific promoter-1 encoding either human coagulation factors IX (hFIX) or X (hFX) into Macaca fascicularis fetuses at ∼0.4 gestation. The initial cohort received 1 × 1012 vector genomes (vgs) of AAV5-hFIX ( n = 5; 0...
December 5, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Yuchun Zhang, Weiming Yi, Peng Fu, Zhihe Li, Nana Wang, Chunyan Tian
Catalytic upgrading of biomass pyrolysis vapors is an effective utilization technology of biomass energy. Based on the disadvantages of commonly used reactors, the V-shaped downer reactors were designed to increase gas-solid two-phase turbulent intensity, contact frequency and then increase the catalytic efficiency in short residence time. The catalytic upgrading of pyrolysis vapors in V-shaped downer reactors in terms of hydrodynamics, chemical reaction and residence time distribution were analyzed by CFD simulation and experiment...
November 27, 2018: Bioresource Technology
Namrata Kabra, Namrata Majhi, Abhishek Desai
PURPOSE: To evaluate the impact of venting incision on graft detachment in Descemet stripping automated endothelial keratoplasty (DSAEK). MATERIALS AND METHODS: Retrospective comparative study on DSAEK procedure performed with ( n = 13) and without ( n = 39) venting incision. Patients were treated with DSAEK for various causes of endothelial decompensation between February 2014 and April 2016. The rate of postoperative graft detachments documented on anterior segment optical coherence tomography was compared in both groups...
September 2018: Oman Journal of Ophthalmology
Hyun-Na Koo, Seung-Hwan Yun, HyunKyung Kim, Gil-Hah Kim
PURPOSE: The objective of the present study was to elucidate the mode of indirect action of electron beam irradiation at the molecular level against a quarantine pest, Spodoptera litura (F.). MATERIALS AND METHODS: Electron beam irradiation (50 - 200 Gy) was applied to S. litura eggs, larvae, pupae and adults, after which the feeding area, body weight, deformity of pupae and adults, ovarian development, expression levels of vitellogenin (Vg) and vitellogenin receptor (VgR) genes, and protein levels were analyzed...
November 30, 2018: International Journal of Radiation Biology
Long Ma, Wanna Zhang, Chen Liu, Lin Chen, Yang Xu, Haijun Xiao, Gemei Liang
Juvenile hormone (JH) represses larval metamorphosis and induces adult reproduction in insects. Methoprene-tolerant (Met) is identified as an intranuclear receptor that mediates JH actions. In the present study, we characterized a Met from the severe agricultural pest, Helicoverpa armigera , namely HaMet. In the larval stage, HaMet is predominantly expressed in the epidermis and midgut, and is upregulated before each molting, whereas in adults HaMet is maximally expressed in the ovary, testis, and fat body...
2018: Frontiers in Physiology
Sezer Okay, Rukiye Çetin, Fatih Karabulut, Cennet Doğan, Süheyla Sürücüoğlu, Aslıhan Kurt Kızıldoğan
Immunogenic potency of the recombinant Erp, HspR, LppX, MmaA4, and OmpA proteins from Mycobacterium tuberculosis (MTB), formulated with Montanide ISA 720 VG adjuvant, was evaluated in BALB/c mice for the first time in this study. The five vaccine formulations, adjuvant, and BCG vaccine were subcutaneously injected into mice, and the sera were collected at days 0, 15, 30, 41, and 66. The humoral and cellular immune responses against vaccine formulations were determined by measuring serum IgG and serum interferon-gamma (IFN-γ) and interleukin-12 (IL-12) levels, respectively...
November 28, 2018: Acta Microbiologica et Immunologica Hungarica
Arit Harvanko, Richard Kryscio, Catherine Martin, Thomas Kelly
BACKGROUND: Little is known about the behavioral effects of non-nicotine ingredients in electronic cigarette liquids. Propylene glycol (PG) and vegetable glycerin (VG) are the most common humectants used in electronic cigarette liquids. These ingredients may influence stimulus effects (e.g., visibility of exhalant, taste, or smell), which have played a role in the abuse liability of conventional cigarettes. In the current study, the stimulus effects of aerosol from liquids varying only in PG and VG content were assessed...
October 18, 2018: Drug and Alcohol Dependence
Hyung Jun Min, Yu Jin Kim
BACKGROUND: To achieve improvements in intramuscular injection, autologous fat grafting, and gluteal artery perforator flaps in infants and children, the relationships of computed tomography (CT)-determined gluteal muscle thickness (MT) and subcutaneous tissue thickness (SCT) with age and body mass index (BMI) were analyzed. METHODS: Gluteal SCT and MT at ventrogluteal (VG) and dorsogluteal (DG) sites were measured in a standardized manner in 350 patients aged 0 to 6 years who had visited a tertiary hospital and had undergone abdominopelvic CT between January 2005 and December 2016...
November 2018: Archives of Plastic Surgery
Min-Soo Kim, Sarah Soh, So Yeon Kim, Min Sup Song, Jin Ha Park
Background: During robot-assisted laparoscopic radical prostatectomy (RALP), steep Trendelenburg position and carbon dioxide pneumoperitoneum are inevitable for surgical exposure, both of which can impair cardiopulmonary function. This study was aimed to compare the effects of pressure-controlled ventilation with volume guarantee (PCV with VG) and 1:1 equal ratio ventilation (ERV) on oxygenation, respiratory mechanics and hemodynamics during RALP. Methods: Eighty patients scheduled for RALP were randomly allocated to either the PCV with VG or ERV group...
2018: International Journal of Medical Sciences
Justin L Poklis, Haley A Mulder, Michelle R Peace
Electronic cigarettes (e-cigarettes) were developed as an alternative method for nicotine delivery and had a significant surge in popularity. E-liquids are formulations used in e-cigarettes, and consist of a ratio of propylene glycol (PG) and vegetable glycerin (VG), a pharmaceutical and/or herbal remedy and, usually, a flavoring agent. Presented is the evaluation of nine cannabidiol (CBD) e-liquids from a single manufacturer for cannabinoids and other psychoactive compounds by Direct Analysis in Real Time Mass Spectrometry (DART-MS) and Gas Chromatography Mass Spectrometry (GC/MS)...
November 1, 2018: Forensic Science International
Congai Zhen, Huipeng Yang, Shudong Luo, Jiaxing Huang, Jie Wu
During reproduction, vitellogenin (Vg), as an egg yolk precursor, is critical in sexually mature females of oviparous species including some insects. The transcription of Vg is usually mediated by hormones such as juvenile hormone (JH), ecdysteroids and some neuropeptides. In this study, the structure of the Vg gene from the bumblebee Bombus lantschouensis, (BlVg) was determined by sequencing and assembly. BlVg was found to be expressed at higher levels in reproductive queens than in virgins by quantitative real-time PCR analysis...
2018: PloS One
Zhe Zhang, Yong Qin, Limin Jia, Xin'an Chen
Reliable fault diagnosis of rolling bearings is an important issue for the normal operation of many rotating machines. Information about the structure dynamics is always hidden in the vibration response of the bearings, and it is often very difficult to extract them correctly due to the nonlinear/chaotic nature of the vibration signal. This paper proposes a new feature extraction model of vibration signals for bearing fault diagnosis by employing a recently-developed concept in graph theory, the visibility graph (VG)...
November 13, 2018: Materials
Ying Shen, Philippa Clarke, Iris N Gomez-Lopez, Alex B Hill, Daniel M Romero, Robert Goodspeed, Veronica J Berrocal, Vg Vinod Vydiswaran, Tiffany C Veinot
OBJECTIVE: To examine the feasibility of using social media to assess the consumer nutrition environment by comparing sentiment expressed in Yelp reviews with information obtained from a direct observation audit instrument for grocery stores. DESIGN: Trained raters used the Nutrition Environment Measures Survey in Stores (NEMS-S) in 100 grocery stores from July 2015 to March 2016. Yelp reviews were available for sixty-nine of these stores and were retrieved in February 2017 using the Yelp Application Program Interface...
November 8, 2018: Public Health Nutrition
Yu-Pu Jing, Hongli An, Shanjing Zhang, Ningbo Wang, Shutang Zhou
In oviparous animals, vitellogenesis is prerequisite to egg production and embryonic growth after oviposition. For success insect vitellogenesis and oogenesis, vitellogenin (Vg) synthesized in the fat body (homologue to vertebrate liver and adipose tissue) must pass through the intercellular channels, a condition known as patency in the follicular epithelium to reach the surface of oocytes. This process is controlled by juvenile hormone (JH) in many insect species, but the underlying mechanisms remain elusive...
November 1, 2018: Journal of Biological Chemistry
Michelle R Peace, Haley A Mulder, Tyson R Baird, Karen E Butler, Alaina K Friedrich, Joseph W Stone, Joseph B McGee Turner, Alphonse Poklis, Justin L Poklis
Electronic cigarettes (e-cigs) deliver nicotine in an aerosol to the user that simulates the smoke of traditional cigarettes purportedly without the pathology of inhaling tobacco smoke due to the absence of combustion. Advanced versions of e-cigs enable the user to potentially moderate the concentration of drug in the aerosol by selecting from a range of voltages on the power supply. A method was developed to trap the aerosol produced by a KangerTech AeroTank, 1.8 Ω preassembled atomizer in order to analyze the concentration of nicotine and to evaluate the constituents of the aerosol at various voltages on the power supply...
October 1, 2018: Journal of Analytical Toxicology
Gamze Inan, Sevil Inal
OBJECTIVES: Invasive procedures are important causes of pain and anxiety during hospitalization. This study aims to evaluate the effect of three different distraction methods on the pain and anxiety levels of children during venipuncture. METHOD: This is a randomized controlled trial that was conducted with 180 children of 6-10 years of age; data was collected in the months of August - November 2016. Participants were randomized in 4 groups; the children in Group 1 watched cartoon movies (CM), the children in Group 2 played video games (VG), the children in Group 3 were distracted by their parents' verbal interactions (PI) whereas no distraction method was used on the children in Group 4 (control group (c)...
October 22, 2018: Clinical Journal of Pain
Youtao Zhu, Zhe Han, Lijun Fu, Chengbu Liu, Dongju Zhang
Understanding the mechanism for the catalyzed cleavage of the β-O-4 ether linkage in lignin is crucial to developing efficient strategies for depolymerizing lignin. In this work, veratrylglycerol-β-guaiacyl ether (VG) was used as a lignin model compound in a theoretical investigation of the mechanism for the cleavage of the β-O-4 bond as catalyzed by the acidic ionic liquid (IL) 1-H-3-methylimidazolium chloride ([HMIM]Cl). The reaction was found to involve two processes-dehydration and hydrolysis-in which the cation functions as a Brønsted acid (donating a proton) and the anion acts as a nucleophile (promoting dehydration) or interacts with the substrate through hydrogen bonding, stabilizing the intermediate...
October 24, 2018: Journal of Molecular Modeling
Elisabeth Spindler, Sandrina Klein, Michael Erhard, Sven Reese, Dorian Patzkéwitsch
OBJECTIVE: Direct comparison of an alternative farrowing pen with a conventional gestation crate in the farrowing area, with a focus on animal welfare and performance. MATERIAL AND METHODS: Over one year, data of 129 sows and their 2109 piglets (thereof 1935 live births) were obtained in seven passes. In total, 65 sows (experimental group, VG) were stalled in a farrowing pen with an openable crate, while 64 sows (control group, KG) were maintained in a closed farrowing pen (gestation crate)...
October 2018: Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere
Benedetta Terziroli Beretta-Piccoli, Paolo Ripellino, Claudio Gobbi, Andreas Cerny, Adriana Baserga, Claudia Di Bartolomeo, Florian Bihl, Gaia Deleonardi, Laura Melidona, Ana Gabriela Grondona, Giorgina Mieli-Vergani, Diego Vergani, Luigi Muratori
The etiology of autoimmune hepatitis (AIH) is unknown, though hepatotropic viruses may be potential triggers. Hepatitis E virus (HEV) infection, an increasingly recognized cause of acute hepatitis, has been misdiagnosed as AIH due to the occurrence of autoantibodies during its acute phase. It has also been suggested that HEV infection may lead to or unmask AIH. The HEV seroprevalence has been ascertained in patients with AIH, but the prevalence of AIH-related autoantibodies in patients with HEV infection has not been systematically tested...
November 2018: Journal of Autoimmunity
Ponsit Sathapondecha, Wilaiwan Chotigeat
In shrimp aquaculture, eyestalk ablation is the only technique that is widely used to accelerate ovarian development. Alternative methods for producing improved ovarian development in broodstock are currently being investigated. Several factors involved in the regulation of ovarian development in shrimp have been investigated. Among these factors, growth factors in the transforming growth factor beta (TGF-β) superfamily have been implicated as playing potential roles in the regulation of gonad development...
October 10, 2018: General and Comparative Endocrinology
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