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https://www.readbyqxmd.com/read/28727291/proteomic-analysis-and-bioluminescent-reporter-gene-assays-to-investigate-effects-of-simulated-microgravity-on-caco-2-cells
#1
Giorgia La Barbera, Anna Laura Capriotti, Elisa Michelini, Susy Piovesana, Maria Maddalena Calabretta, Riccardo Zenezini Chiozzi, Aldo Roda, Aldo Laganà
Microgravity is one of the most important features in spaceflight. Previous evidence from in-vitro studies has shown that significant changes occur under simulated microgravity. For this reason, human colon adenocarcinoma Caco-2 cells were selected as cell model of intestinal epithelial barrier and their response to altered gravity conditions was investigated, especially on the protein level. In this study, we combined label-free shotgun proteomics and bioluminescent reporter gene assays to identify key proteins and pathways involved in the response of Caco-2 cells under reference and microgravity conditions...
July 20, 2017: Proteomics
https://www.readbyqxmd.com/read/28726958/wasabia-japonica-is-a-potential-functional-food-to-prevent-colitis-via-inhibiting-the-nf-%C3%AE%C2%BAb-signaling-pathway
#2
Ju-Hee Kang, Seungho Choi, Jeong-Eun Jang, Prakash Ramalingam, Young Tag Ko, Sun Yeou Kim, Seung Hyun Oh
Inflammatory bowel diseases (IBDs), including Crohn's disease (CD) and ulcerative colitis (UC), are prevalent and debilitating health problems worldwide. Many types of drugs are used to treat IBDs, but they exhibit adverse effects such as vomiting, nausea, abdominal pain, diarrhea, etc. In order to overcome the limitations of current therapeutic drugs, scientists have searched for functional foods from natural resources. In this study, we investigated the anti-colitic effects of Wasabia japonica extract in a DSS-induced colitis model...
July 20, 2017: Food & Function
https://www.readbyqxmd.com/read/28711686/hydrogel-elasticity-and-microarchitecture-regulate-dental-derived-mesenchymal-stem-cells-host-immune-system-cross-talk
#3
Sahar Ansari, Chider Chen, Mohammad Mahdi Hasani-Sarabadi, Bo Yu, Homayoun H Zadeh, Benjamin M Wu, Alireza Moshaverinia
The host immune system (T-lymphocytes and their pro-inflammatory cytokines) has been shown to compromise bone regeneration ability of mesenchymal stem cells (MSCs). We have recently shown that hydrogel, used as an encapsulating biomaterial affects the cross-talk among host immune cells and MSCs. However, the role of hydrogel elasticity and porosity in regulation of cross-talk between dental-derived MSCs and immune cells is unclear. In this study, we demonstrate that the modulus of elasticity and porosity of the scaffold influence T-lymphocyte-dental MSC interplay by regulating the penetration of inflammatory T cells and their cytokines...
July 12, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28707661/-the-effects-of-interleukin-1-beta-and-gamma-quantum-braking-radiation-on-mesenchymal-progenitor-cells
#4
A E Bigildeev, E A Zezina, N J Drize
In murine bone-marrow stromal microenvironment cells and in human multipotent mesenchymal stromal cells (MMSCs), proinflammatory cytokine interleukin-1 beta (IL-1β) serves as a growth factor. In murine bone tissue, IL-1β expression increases in vivo after irradiation. Here, we have presented our evaluation of the effects of exogenous IL-1β on the expression of NF-kB transcription factors in human MMSCs and stromal layer cells of murine long-term bone marrow cultures (LTBMCs). The cytokine signaling pathway was also activated in murine LTBMC by braking electron radiation in doses of 3-12 Gy...
May 2017: Molekuliarnaia Biologiia
https://www.readbyqxmd.com/read/28699353/radiation-hormesis-the-link-to-nanomolar-hydrogen-peroxide
#5
Helmut Sies, Ludwig E Feinendegen
Hydrogen peroxide is a stable product of water radiolysis, occurring at nanomolar concentration upon low dose ionizing radiation (<100 mGy). In view of the recent recognition of H2O2 as a central redox signaling molecule which, likewise, is maintained in the nanomolar range in cells, we propose a role for H2O2 in radiation hormesis. Low dose ionizing radiation is capable of utilizing known molecular redox master switches such as Nrf2/Keap1 or NF-kB/IkB to effect adaptive resistance. This leads to the hypothesis that, as a normal component of the exposome, low dose ionizing radiation mediates hormetic effects by hydrogen peroxide signaling...
July 12, 2017: Antioxidants & Redox Signaling
https://www.readbyqxmd.com/read/28698002/the-flavonol-enriched-cistus-albidus-chloroform-extract-possesses-in-vivo-anti-inflammatory-and-anti-nociceptive-activity
#6
Ouahiba Tahiri, Dina Atmani-Kilani, Susana Sanchez-Fidalgo, Marina Aparicio-Soto, Catalina Alarcón-de-la-Lastra, Enrique Barrajón-Catalán, Vicente Micol, Djebbar Atmani
ETHNOPHARMACOLOGICAL RELEVANCE: Cistus albidus L. (Cistaceae) has been traditionally used to treat various inflammatory diseases, but no systematic studies on the anti-inflammatory and anti-nociceptive actions of C. albidus and its putative mechanism have been reported. We aimed to explore the anti-inflammatory and anti-nociceptive effects of this plant and to characterize its polyphenolic composition by liquid chromatography coupled to mass spectrometry (MS). MATERIALS AND METHODS: A chloroform extract derived from C...
July 8, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28689797/pudilan-xiaoyan-oral-liquid-alleviates-lps-induced-respiratory-injury-through-decreasing-nitroxidative-stress-and-blocking-tlr4-activation-along-with-nf-%C3%AE-b-phosphorylation-in-mice
#7
Liang Feng, Nan Yang, Chao Li, Gang Tian, Jing Wang, Zi-Bo Dong, Xiao-Bin Jia, Liu-Qing Di
ETHNOPHARMACOLOGICAL RELEVANCE: Pudilan xiaoyan oral liquid (PDL), collected in Chinese Pharmacopoeia, has been used clinically for treating inflammatory diseases such as upper respiratory tract infection diseases. However, its potential anti-inflammation and the mechanism are still unclear. MATERIALS AND METHODS: lipopolysaccharide (LPS) was used to induce respiratory inflammation of mice by intratracheal administration. UPLC/MS was performed for components analysis of PDL...
July 6, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28687648/host-specificity-of-segmented-filamentous-bacteria-sfb-flagellins-affects-their-interaction-patterns-with-mouse-ileal-mucosal-proteins
#8
Huahai Chen, Yeshi Yin, Yanling Wang, Xin Wang, Charlie Xiang
Segmented filamentous bacteria (SFB) are known modulators of the mammalian immune system. Currently, the technology for investigating SFB culture in vitro is immature, and as a result, the mechanisms of SFB colonization and immune regulation are not yet fully elucidated. In this study, we investigated the gene diversity and host specificity of SFB flagellin genes. The flic1 and flic2 genes are relatively conserved, while the flic3 and flic4 genes are more variable, especially at the central and C-terminal regions...
July 7, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28680569/ppar%C3%AE-attenuates-hypoxia-induced-hypertrophic-transcriptional-pathways-in-the-heart
#9
Abubakr Chaudhry, Kristal A Carthan, Bum-Yong Kang, John Calvert, Roy L Sutliff, C Michael Hart
Chronic hypoxia-induced pulmonary hypertension (PH) is characterized by increased pressure and resistance in the pulmonary vasculature and hypertrophy of the right ventricle (RV). The transcription factors, nuclear factor activated T-cells (NFAT), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB/p65) contribute to RV hypertrophy (RVH). Because peroxisome proliferator-activated receptor gamma (PPARγ) activation attenuates hypoxia-induced PH and RVH, we hypothesized that PPARγ inhibits activation of RV hypertrophic transcriptional signaling mechanisms...
March 2017: Pulmonary Circulation
https://www.readbyqxmd.com/read/28680537/znf545-suppresses-human-hepatocellular-carcinoma-growth-by-inhibiting-nf-kb-signaling
#10
Weili Yang, Shuai Yang, Meiying Zhang, Dan Gao, Tao He, Mingzhou Guo
Hepatocellular carcinoma (HCC) is one of the most common cancers and the second leading cause of cancer related death worldwide. ZNF545 is located in the chromosome 19q13.13, which is frequent loss of heterozygosity in human astrocytoma. Methylation of ZNF545 was found frequently in a few kinds of cancers. While the function of ZNF545 in human HCC remains unclear. The purpose of this study is to explore the function and mechanism of ZNF545 in human HCC. Restoration of ZNF545 expression suppressed cell proliferation, migration and invasion, induced G1/S arrest and apoptosis in SNU449 and Huh7 cells...
March 2017: Genes & Cancer
https://www.readbyqxmd.com/read/28679031/effect-of-social-interactions-on-hippocampal-protein-expression-in-animal-dominant-and-submissive-model-of-behavioral-disorders
#11
Natalia Borovok, Elimelech Nesher, Michal Reichenstein, Tatiana Tikhonova, Yishai Levin, Albert Pinhasov, Izhak Michaelevski
Psychiatric conditions often arise from social interactions, necessary for optimal mental functioning. Dominance and submissiveness are opposite poles of behavior, stemming from processes of social interactions between members inside one group or species. Extreme dominance/submissiveness expressions in humans is accompanied by mental impairments, mania or depression. Here, taking advantage of selectively bred dominant and submissive mice, we assessed protein expression profiles in the social interaction context...
July 5, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/28676205/small-molecules-to-the-rescue-inhibition-of-cytokine-signaling-in-immune-mediated-diseases
#12
REVIEW
Massimo Gadina, Nathalia Gazaniga, Laura Vian, Yasuko Furumoto
Cytokines are small, secreted proteins associated with the maintenance of immune homeostasis but also implicated with the pathogenesis of several autoimmune and inflammatory diseases. Biologic agents blocking cytokines or their receptors have revolutionized the treatment of such pathologies. Nonetheless, some patients fail to respond to these drugs or do not achieve complete remission. The signal transduction originating from membrane-bound cytokine receptors is an intricate network of events that lead to gene expression and ultimately regulate cellular functionality...
July 1, 2017: Journal of Autoimmunity
https://www.readbyqxmd.com/read/28673588/biological-functions-of-melatonin-in-relation-to-pathogenesis-of-oral-lichen-planus
#13
Ponlatham Chaiyarit, Kirawut Luengtrakoon, Worraned Wannakasemsuk, Vilasinee Vichitrananda, Poramaporn Klanrit, Doosadee Hormdee, Rajda Noisombut
Oral lichen planus (OLP) is considered as a chronic inflammatory immune-mediated disease causing oral mucosal damage and ulcerations. Accumulated data support the involvement of cell-mediated immune dysfunction in the development of OLP. However, the connection between neuroendocrine system and oral immune response in OLP patients has never been clarified. Melatonin is considered as a major chronobiotic hormone produced mainly by the pineal gland. This gland is recognized as a regulator of circadian rhythm and a sensor in the immune response through the NF-kB transduction pathway...
July 2017: Medical Hypotheses
https://www.readbyqxmd.com/read/28670514/delayed-liver-regeneration-after-partial-hepatectomy-in-aged-nos2-knockout-mice
#14
Deming Li, Jun Li, Gaiping Wang, Yanli Qin, Zhipeng Niu, Ziwei Li, Cunshuan Xu
OBJECTIVE: Patients over 60 years of age have higher mortality and morbidity after major liver resections. Nitric oxide (NO) derived from the catalytic activity of Nos2 plays a beneficial role in liver regeneration (LR) after partial hepatectomy (PH). In this experiment, we evaluated the effect of Nos2 knockout (KO) on LR in aged mice after PH. MATERIALS AND METHODS: In this experimental study, 52 two-year-old Nos2 KO and 46 the same age wild-type (WT) C57BL/6J mice were subjected to 2/3 PH...
July 2017: Cell Journal
https://www.readbyqxmd.com/read/28664719/differences-in-the-expression-of-selected-genes-associated-with-cell-stress-response-in-a-ptsd-patient-and-in-healthy-individuals-a-case-study
#15
Anna Rasmus, Paweł Półrola, Jolanta Góral-Półrola, Małgorzata Żychowska, Andrzej Mirski, Henryk Knapik
<b>Objective.</b> The aim of the study was determination of the level in expression genes associated with cell stress response in a patient with PTSD. <b>Case study.</b> A 57-year-old PTSD patient, A.P., was the subject of the study. Blood samples were taken from the ulnar vein to assess the relative expression of the tested genes in leukocytes via a quantitative reverse transcription polymerase chain reaction (qRT-PCR). <b>Results.</b> A considerably decreased level in the expression HSF-1, NF-kB, Hif-1 and IL6 m-RNA was observed in the patient when compared to the control group...
June 12, 2017: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/28663093/heat-stress-induced-neuroinflammation-and-aberration-in-monoamine-levels-in-hypothalamus-are-associated-with-temperature-dysregulation
#16
Nishant Ranjan Chauhan, Medha Kapoor, Laxmi Prabha Singh, Rajinder Kumar Gupta, Ramesh Chand Meena, Rajkumar Tulsawani, Sarita Nanda, Shashi Bala Singh
Heat Stress (HS) induces diverse pathophysiological changes, which include brain ischemia, oxidative stress and neuronal damage. The present study was undertaken with the objective to ascertain whether neuroinflammation in Hypothalamus (HTH) caused under HS affects monoamine levels and hence, its physiological role in thermoregulation. Rats were exposed to HS in a heat simulation environmental chamber (Ambient temperature, Ta=45±0.5°C and Relative Humidity, RH=30±10%) with real-time measurement of core temperature (Tc) and skin temperature (Ts)...
June 27, 2017: Neuroscience
https://www.readbyqxmd.com/read/28662337/danshen-attenuates-osteoarthritis-related-cartilage-degeneration-through-inhibition-of-nf-%C3%AE%C2%BAb-signaling-pathway-in-vivo-and-in-vitro
#17
Xilin Xu, Hang Lv, Xiaodong Li, Hui Su, Xiaofeng Zhang, Jun Yang
Danshen is a traditional Chinese medicine herb that can alleviate osteoarthritis (Soder et al.) symptoms in animals. However, the underlying mechanisms remain poorly understood and require further investigation. In this study, rabbits with experimental OA were given intra-articular injection of Danshen (0.7 mL/day) for five weeks. In addition to attenuating the cartilage degeneration of OA rabbits, Danshen decreased the expression and activity of matrix metalloproteinase 9 (MMP-9) and MMP-13, whereas increased that of their natural inhibitors, tissue inhibitor of matrix metalloproteinase 1 (TIMP-1) and TIMP-2...
June 29, 2017: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28661485/osteoblastic-heparan-sulfate-glycosaminoglycans-control-bone-remodeling-by-regulating-wnt-signaling-and-the-crosstalk-between-bone-surface-and-marrow-cells
#18
Rafik Mansouri, Yohann Jouan, Eric Hay, Claudine Blin-Wakkach, Monique Frain, Agnès Ostertag, Carole Le Henaff, Caroline Marty, Valérie Geoffroy, Pierre J Marie, Martine Cohen-Solal, Dominique Modrowski
Stimulating bone formation is an important challenge for bone anabolism in osteoporotic patients or to repair bone defects. The osteogenic properties of matrix glycosaminoglycans (GAGs) have been explored; however, the functions of GAGs at the surface of bone-forming cells are less documented. Syndecan-2 is a membrane heparan sulfate proteoglycan that is associated with osteoblastic differentiation. We used a transgenic mouse model with high syndecan-2 expression in osteoblasts to enrich the bone surface with cellular GAGs...
June 29, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28659803/indoxyl-sulfate-affects-glial-function-increasing-oxidative-stress-and-neuroinflammation-in-chronic-kidney-disease-interaction-between-astrocytes-and-microglia
#19
Simona Adesso, Tim Magnus, Salvatore Cuzzocrea, Michela Campolo, Björn Rissiek, Orlando Paciello, Giuseppina Autore, Aldo Pinto, Stefania Marzocco
Indoxyl sulfate (IS) is a protein-bound uremic toxin resulting from the metabolism of dietary tryptophan which accumulates in patients with impaired renal function, such as chronic kidney disease (CKD). IS is a well-known nephrovascular toxin but little is known about its effects on central nervous system (CNS) cells. Considering the growing interest in the field of CNS comorbidities in CKD, we studied the effect of IS on CNS cells. IS (15-60 μM) treatment in C6 astrocyte cells increased reactive oxygen species release and decreased nuclear factor (erythroid-derived 2)-like 2 (Nrf2) activation, and heme oxygenase-1 (HO-1) and NAD(P)H dehydrogenase quinone 1 expression...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28651126/tnf-%C3%AE-exerts-cytotoxic-effects-on-multidrug-resistant-breast-cancer-mcf-7-mx-cells-via-a-non-apoptotic-death-pathway
#20
Morteza Ghandadi, Javad Behravan, Khalil Abnous, Melika Ehtesham Gharaee, Fatemeh Mosaffa
Tumor necrosis factor-α (TNF-α) is a cytokine involved in the various physiopathological processes such as autoimmune disorders and inflammation related diseases. Some multidrug resistant (MDR) cancer cell lines including MCF-7/MX are more vulnerable to cytotoxic effects of TNF-α than their parental lines. In this study, breast cancer cell line MCF-7 and its MDR derivative MCF-7/MX were exposed to TNF-α afterward various downstream signaling mediators of TNF-α were analyzed. Although, treatment of MCF-7 cells with TNF-α activated NF-kB and caused RIP1 ubiquitination, TNF-α exposure led to JNK and RIP1 phosphorylation in MCF-7/MX cells...
June 23, 2017: Cytokine
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