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miR-181a

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https://www.readbyqxmd.com/read/28224609/mir-181-elevates-akt-signaling-by-co-targeting-phlpp2-and-inpp4b-phosphatases-in-luminal-breast-cancer
#1
Michaela Strotbek, Simone Schmid, Ismael Sànchez-Gonzàlez, Melanie Boerries, Hauke Busch, Monilola A Olayioye
The PI3K-Akt pathway is one of the most commonly dysregulated cancer-associated signaling pathways. Here we report an oncogenic function for the miR-181 family in luminal breast cancer cells that involves Akt hyperactivation. We show that miR-181a and miR-181d post-transcriptionally suppress the expression of PHLPP2 and INPP4B phosphatases, resulting in elevated growth factor-induced Akt phosphorylation. Ectopic expression of miR-181a and miR-181d promoted S-phase entry and cell proliferation, which was reversed by pharmacological Akt inhibition...
February 22, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28192189/mir-425-regulates-inflammatory-cytokine-production-in-cd4-t-cells-via-n-ras-upregulation-in-primary-biliary-cholangitis
#2
Ryo Nakagawa, Ryosuke Muroyama, Chisato Saeki, Kaku Goto, Yoshimi Kaise, Kazuhiko Koike, Masanori Nakano, Yasuo Matsubara, Keiko Takano, Sayaka Ito, Masayuki Saruta, Naoya Kato, Mikio Zeniya
BACKGROUND & AIM: Primary biliary cholangitis (PBC) is an autoimmune liver disease of unknown pathogenesis. Consequently, therapeutic targets for PBC have yet to be identified. Because CD4(+) T cells play a pivotal role in immunological dysfunction observed in PBC, we analysed microRNA (miRNA) and mRNA expression in CD4(+) T cells to investigate PBC pathogenesis and identify novel therapeutic targets. METHODS: We performed integral miRNA and mRNA analysis of 14 PBC patients and 10 healthy controls using microarray and quantitative real time polymerase chain reaction (qRT-PCR) with gene set enrichment analysis...
February 9, 2017: Journal of Hepatology
https://www.readbyqxmd.com/read/28184923/mir-181a-5p-an-inducer-of-wnt-signaling-facilitates-cell-proliferation-in-acute-lymphoblastic-leukemia
#3
Xiaoming Lyu, Jinbang Li, Xi Yun, Rui Huang, Xubin Deng, Yingping Wang, Youming Chen, Gang Xiao
Uncontrolled Wnt signaling causes leukemia. Inactivation of Wnt antagonists could play an important role in leukemia progression by activating the Wnt/β-catenin pathway. Wnt inhibitory factor-1 (WIF1) is one of the important Wnt antagonists. Few miRNAs have been reported to directly target this gene in hematopoiesis. Here, we observed that miR-181a-5p expression was markedly overexpressed in several leukemia cell lines and acute lymphoblastic leukemia (ALL) samples compared with that noted in normal peripheral blood mononuclear cells...
March 2017: Oncology Reports
https://www.readbyqxmd.com/read/28181149/genome-wide-sequencing-reveals-micrornas-downregulated-in-cerebral-cavernous-malformations
#4
Souvik Kar, Kiran Kumar Bali, Arpita Baisantry, Robert Geffers, Amir Samii, Helmut Bertalanffy
Cerebral cavernous malformations (CCM) are vascular lesions associated with loss-of-function mutations in one of the three genes encoding KRIT1 (CCM1), CCM2, and PDCD10. Recent understanding of the molecular mechanisms that lead to CCM development is limited. The role of microRNAs (miRNAs) has been demonstrated in vascular pathologies resulting in loss of tight junction proteins, increased vascular permeability and endothelial cell dysfunction. Since the relevance of miRNAs in CCM pathophysiology has not been elucidated, the primary aim of the study was to identify the miRNA-mRNA expression network associated with CCM...
February 8, 2017: Journal of Molecular Neuroscience: MN
https://www.readbyqxmd.com/read/28168095/identification-of-four-potential-predicting-mirna-biomarkers-for-multiple-myeloma-from-published-datasets
#5
Tian Xiang, Ai-Xin Hu, Peng Sun, Gao Liu, Gang Liu, Yan Xiao
BACKGROUND: Multiple myeloma is a cancer which has a high occurrence rate and causes great injury to people worldwide. In recent years, many studies reported the effects of miRNA on the appearance of multiple myeloma. However, due to the differences of samples and sequencing platforms, a large number of inconsistent results have been generated among these studies, which limited the cure of multiple myeloma at the miRNA level. METHODS: We performed meta-analyses to identify the key miRNA biomarkers which could be applied on the treatment of multiple myeloma...
2017: PeerJ
https://www.readbyqxmd.com/read/28145491/altered-expression-of-mir-181a-and-mir-146a-does-not-change-the-expression-of-surface-ncrs-in-human-nk-cells
#6
Mona Rady, Carsten Watzl, Maren Claus, Ola Khorshid, Laila Mahran, Khaled Abou-Aisha
MicroRNAs (miRNAs) play an important role in regulating gene expression and immune responses. Of interest, miR-181a and miR-146a are key players in regulating immune responses and are among the most abundant miRNAs expressed in NK cells. Bioinformatically, we predicted miR-181a to regulate the expression of the natural cytotoxicity receptor NCR2 by seeded interaction with the 3'-untranslated region (3'-UTR). Whereas, miR-146a expression was not significantly different (P = 0.7361), miR-181a expression was, on average 10-fold lower in NK cells from breast cancer patients compared to normal subjects; P < 0...
February 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28133358/1-25-dihydroxyvitamin-d3-does-not-affect-microrna-expression-when-suppressing-human-th17-differentiation
#7
Jian Huang, Zibin Liang, Ying Kuang, Fujie Jia, Yaqi Yang, Miaomiao Kang, Muke Xie, Feng Li
BACKGROUND Vitamin D is an import regulator of T helper 17 (Th17) differentiation, but our understanding of the underlying mechanisms remains limited. In the present study, we aimed to detect the expression levels of microRNAs (miRNAs) during human Th17 differentiation and evaluate the effects of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), the bioactive form of vitamin D, on Th17 differentiation and miRNA expression. MATERIAL AND METHODS We cultured human peripheral blood mononuclear cells (PBMC) in vitro and activated them with anti-CD3 and anti-CD28 antibodies in the presence of Th17-promoting cytokines interleukin (IL)-23, IL-1β, TGF-β1, and IL-6 for 72 hours...
January 30, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28131804/downregulation-of-autophagy-related-gene-atg5-and-gabarap-expression-by-ifn-%C3%AE-1-contributes-to-its-anti-hcv-activity-in-human-hepatoma-cells
#8
Xu Li, Yu Li, Shoucai Fang, Jinming Su, Junjun Jiang, Bingyu Liang, Jiegang Huang, Bo Zhou, Ning Zang, Wenzhe Ho, Jieliang Li, Yiping Li, Hui Chen, Li Ye, Hao Liang
Type-III interferon (IFN-λ), the most recently discovered family of IFNs, shares common features with type I IFNs, but also has many distinctive activities. It is not clear that whether IFN-λ has additional antiviral mechanisms. In this study, we investigated the effects of IFN-λ on autophagy, a cellular process closely related to hepatitis C virus (HCV) infection in human hepatoma Huh7 cells. Our results showed that IFN-λ1 treatment inhibit autophagic activity in Huh7 cells, as evidenced by the decreased expression of microtubule-associated protein 1 light chain 3B (LC3B)-II and conversion of LC3B-I to LC3B-II, decreased formation of GFP-LC3 puncta and accumulation of autophagosomes...
January 26, 2017: Antiviral Research
https://www.readbyqxmd.com/read/28121023/lncrna-casc2-interacts-with-mir-181a-to-modulate-glioma-growth-and-resistance-to-tmz-through-pten-pathway
#9
Liao Yiwei, Shen Liangfang, Zhao Haiting, Liu Qing, Fu Jun, Guo Yong, Peng Renjun, Cheng Lei
Temozolomide (TMZ)-based chemotherapy is a standard strategy for glioma, while chemoresistance remains a major therapeutic challenge. Recent evidence highlights the crucial regulatory roles of long non-coding RNAs (lncRNA) in tumor biology. However, the roles and regulatory mechanisms of lncRNA cancer susceptibility candidate 2 (CASC2), in glioma tumorigenesis and chemoresistance are poorly understood. In this study, CASC2 expression was down-regulated in glioma tissues and cell lines, and was related to a clinicopathologic features and shorter survival time...
January 25, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28119261/roles-of-micrornas-in-immunopathogenesis-of-non-alcoholic-fatty-liver-disease-revealed-by-integrated-analysis-of-microrna-and-mrna-expression-profiles
#10
Yu-Jun Zhang, Ying Hu, Jing Li, Yu-Jing Chi, Wei-Wei Jiang, Feng Zhang, Yu-Lan Liu
BACKGROUND: The integrative analysis of microRNA and mRNA expression profiles can elucidate microRNA-targeted gene function. We used this technique to elucidate insights into the immunological pathology of non-alcoholic fatty liver disease (NAFLD). METHODS: We analyzed differentially expressed microRNA and mRNA expression profiles of CD4+ T lymphocytes from the liver and mesenteric lymph nodes (MLNs) of mice with NAFLD using microarrays and RNA sequencing. Normal mice were used as controls...
February 2017: Hepatobiliary & Pancreatic Diseases International: HBPD INT
https://www.readbyqxmd.com/read/28105518/associations-between-hepatic-mirna-expression-liver-triacylglycerols-and-gut-microbiota-during-metabolic-adaptation-to-high-fat-diet-in-mice
#11
Vincent Blasco-Baque, Berengère Coupé, Aurelie Fabre, Sandra Handgraaf, Pierre Gourdy, Jean-François Arnal, Michael Courtney, Carole Schuster-Klein, Beatrice Guardiola, François Tercé, Rémy Burcelin, Matteo Serino
AIMS/HYPOTHESIS: Despite the current pandemic of metabolic diseases, our understanding of the diverse nature of the development of metabolic alterations in people who eat a high-fat diet (HFD) is still poor. We recently demonstrated a cardio-metabolic adaptation in mice fed an HFD, which was characterised by a specific gut and periodontal microbiota profile. Since the severity of hepatic disease is characterised by specific microRNA (miRNA) signatures and the gut microbiota is a key driver of both hepatic disease and miRNA expression, we analysed the expression of three hepatic miRNA and studied their correlation with hepatic triacylglycerol content and gut microbiota...
January 19, 2017: Diabetologia
https://www.readbyqxmd.com/read/28103766/long-term-exposure-to-imatinib-mesylate-downregulates-hippo-pathway-and-activates-yap-in-a-model-of-chronic-myelogenous-leukemia
#12
Anna Chorzalska, Javier Flores Kim, Karim Roder, Alexander Tepper, Nagib Ahsan, R Shyama Prasad Rao, Adam J Olszewski, Xiaoqing Yu, Dmitry Terentyev, John Morgan, Diana O Treaba, Ting Zhao, Olin Liang, Philip Gruppuso, Patrycja Dubielecka
Despite the success of tyrosine kinase inhibitor (TKI) therapy in chronic myelogenous leukemia (CML), leukemic stem/progenitor cells remain detectable even in the state of deep molecular remission. Mechanisms that allow them to persist despite continued kinase inhibition remain unclear. We have previously shown that prolonged exposure to imatinib mesylate results in dysregulation of Akt/Erk 1/2 signaling, upregulation of miR-181a, enhanced adhesiveness, and resistance to high imatinib mesylate. In order to characterize the molecular basis and reversibility of those effects, we applied gene and protein expression analysis, quantitative phosphoproteomic, and direct miR-181a inhibition to our cellular model of CML cells subjected to prolonged exposure to imatinib mesylate...
January 19, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28086904/the-lncrna-crnde-promotes-colorectal-cancer-cell-proliferation-and-chemoresistance-via-mir-181a-5p-mediated-regulation-of-wnt-%C3%AE-catenin-signaling
#13
Peng Han, Jing-Wen Li, Bo-Miao Zhang, Jia-Chen Lv, Yong-Min Li, Xin-Yue Gu, Zhi-Wei Yu, Yun-He Jia, Xue-Feng Bai, Li Li, Yan-Long Liu, Bin-Bin Cui
BACKGROUND: With more than 600,000 mortalities each year, colorectal cancer (CRC) is the third most commonly diagnosed type of cancer worldwide. Recently, mechanisms involving noncoding RNAs have been implicated in the development of CRC. METHODS: We examined expression levels of lncRNA CRNDE and miR-181a-5p in 64 cases of CRC tissues and cell lines by qRT-PCR. Gain-of-function and loss-of-function assays were performed to examine the effect of CRNDE and miR-181a-5p on proliferation and chemoresistance of CRC cells...
January 13, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28077323/high-glucose-down-regulates-microrna-181a-5p-to-increase-pro-fibrotic-gene-expression-by-targeting-early-growth-response-factor-1-in-hk-2-cells
#14
Ping Xu, Mei-Ping Guan, Jian-Gang Bi, Dan Wang, Zong-Ji Zheng, Yao-Ming Xue
Tubulointerstitial fibrosis (TIF) plays an important role in the progression of renal fibrosis in diabetic nephropathy (DN). Accumulating evidence supports a crucial effect of early growth response factor 1 (Egr1) on renal fibrosis in DN, but the underlying mechanisms are not entirely clear. Here, we explored the aggravating role of Egr1 and identified microRNA-181a-5p (miR-181a-5p) as an upstream regulator of Egr1 in TIF of DN. We demonstrated that overexpression of Egr1 enhanced, whereas small interfering RNA targeting Egr1 decreased the expressions of transforming growth factor β1 (TGF-β1) and fibrosis-related genes including fibronectin and collagen I in human proximal tubule cell line (HK-2) cells...
February 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28053323/hbx-promotes-cell-proliferation-by-disturbing-the-cross-talk-between-mir-181a-and-pten
#15
Yi Tian, Xinqiang Xiao, Xing Gong, Feng Peng, Yun Xu, Yongfang Jiang, Guozhong Gong
Hepatitis B virus X protein (HBx) is involved in the initiation and progression of hepatocellular carcinoma (HCC). However, the mechanism is still needed to be elucidated. In this study, we explored the relationship between HBx and microRNA and their roles in hepato-carcinogenesis. Firstly, by global microarray-based microRNA profiling and qRT-PCR, we found miR-181a was strongly up-regulated in HepG2.2.15 cells (HBV positive) and pHBV1.3-expressing HepG2 cells, and HBx played a major role in it. Secondly, reduced PTEN protein expression in the presence of HBx was aslo mediated by miR-181a, and in the Luciferase reporter system, miR-181a inhibited the PTEN translation by binding the PTEN 3'-untranslated-region (UTR), and PTEN protein was decreased when epigenetic expression of miR-181a and rescued by knocking down miR-181a...
January 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28043911/mir-181a-5p-promotes-the-progression-of-gastric-cancer-via-rassf6-mediated-mapk-signalling-activation
#16
Yushuai Mi, Dongyuan Zhang, Weiliang Jiang, Junyong Weng, Chongzhi Zhou, Kejian Huang, Huamei Tang, Yang Yu, Xisheng Liu, Weiyingqi Cui, Meng Zhang, Xiaofeng Sun, Zongguang Zhou, Zhihai Peng, Senlin Zhao, Yugang Wen
We previously discovered that Ras association domain family member 6 (RASSF6) was downregulated and predicted poor prognosis in GC patients. However, the mechanisms of the down regulation of RASSF6 in GC remained unclear. Increasing evidence indicates that dysregulation of microRNAs promotes the progression of cancer through the repression of tumour suppressors. Here, we identified miR-181a-5p as a novel regulator of RASSF6 in GC. Functionally, ectopic expression or silencing of miR-181a-5p, respectively, promoted or inhibited GC cell proliferation, colony formation and cell cycle transition, as well as enhanced or prevented the invasion, metastasis of GC cells and epithelial to mesenchymal transition of GC cells in vitro and in vivo...
March 28, 2017: Cancer Letters
https://www.readbyqxmd.com/read/27999452/microrna-regulation-of-endothelial-junction-proteins-and-clinical-consequence
#17
REVIEW
Yugang Zhuang, Hu Peng, Victoria Mastej, Weiguo Chen
Cellular junctions play a critical role in structural connection and signal communication between cells in various tissues. Although there are structural and functional varieties, cellular junctions include tight junctions, adherens junctions, focal adhesion junctions, and tissue specific junctions such as PECAM-1 junctions in endothelial cells (EC), desmosomes in epithelial cells, and hemidesmosomes in EC. Cellular junction dysfunction and deterioration are indicative of clinical diseases. MicroRNAs (miRNA) are ~20 nucleotide, noncoding RNAs that play an important role in posttranscriptional regulation for almost all genes...
2016: Mediators of Inflammation
https://www.readbyqxmd.com/read/27997916/circulating-mir-181a-as-a-potential-novel-biomarker-for-diagnosis-of-acute-myocardial-infarction
#18
Jianbing Zhu, Kang Yao, Qian Wang, Junjie Guo, Hongtao Shi, Leilei Ma, Haibo Liu, Wei Gao, Yunzeng Zou, Junbo Ge
BACKGROUND: In this study, we tested the hypothesis that miR-181a levels increase during acute myocardial infarction. We investigated circulating miR-181a as a potential novel biomarker for early diagnosis of acute myocardial infarction (AMI). METHODS: From June 2014 to June 2016, 120 consecutive eligible patients with AMI (n = 60) or unstable angina (UA; n = 60) and 60 control subjects were enrolled. Plasma miR-181a levels were determined by quantitative reverse transcriptase-polymerase chain reaction...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27979502/digital-pcr-quantification-of-mir-30c-and-mir-181a-as-serum-biomarkers-for-duchenne-muscular-dystrophy
#19
Monica Llano-Diez, Carlos Ignacio Ortez, Judit Armas Gay, Lidia Álvarez-Cabado, Cristina Jou, Julita Medina, Andres Nascimento, Cecilia Jimenez-Mallebrera
Circulating microRNAs (miRs/miRNAs) are being used as non-invasive biomarkers for diagnosis, prognosis and efficiency of clinical trials. However, to exploit their potential it is necessary to improve and standardize their detection. In a previous study, we identified two microRNAs, miR-30c and miR-181a, that appear to be key regulators of muscular dystrophy. We hypothesized that they could represent useful biomarkers of Duchenne and Becker muscular dystrophies (DMD and BMD). The objective of this study was to assess the absolute levels of miR-30c and miR-181a in sera of DMD and BMD patients using digital PCR (a robust technique for precise and direct quantification of small amounts of nucleic acids without standard curves and external references), and investigate the correlation between miR-30c and miR-181a expressions and several clinical parameters...
November 11, 2016: Neuromuscular Disorders: NMD
https://www.readbyqxmd.com/read/27935974/epigallocatechin-3-gallate-upregulates-mir-221-to-inhibit-osteopontin-dependent-hepatic-fibrosis
#20
M L Arffa, M A Zapf, A N Kothari, V Chang, G N Gupta, X Ding, M M Al-Gayyar, W Syn, N M Elsherbiny, P C Kuo, Z Mi
Osteopontin (OPN) promotes hepatic fibrosis, and developing therapies targeting OPN expression in settings of hepatic injury holds promise. The polyphenol epigallocatechin-3-gallate (EGCG), found in high concentrations in green tea, downregulates OPN expression through OPN mRNA degradation, but the mechanism is unknown. Previous work has shown that microRNAs can decrease OPN mRNA levels, and other studies have shown that EGCG modulates the expression of multiple microRNAs. In our study, we first demonstrated that OPN induces hepatic stellate cells to transform into an activated state...
2016: PloS One
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