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Xia Shan, Huo Zhang, Lan Zhang, Xin Zhou, Tongshan Wang, JinYing Zhang, Yongqian Shu, Wei Zhu, Wei Wen, Ping Liu
Dysregulated microRNAs (miRNAs) in the plasma of patients with lung squamous cell carcinoma (LSCC) might serve as biomarkers for LSCC diagnosis. The expression of miRNAs was performed using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) on the basis of Exiqon panels in the initial screening phase including three male LSCC pool samples and one normal control (NC) pool sample (per 10 samples were pooled as one pool sample). After the training (32 LSCC vs. 31 NCs), the testing (55 LSCC vs...
April 19, 2018: Cancer Medicine
Nan Wang, Yaling Feng, Jianjuan Xu, Jinfang Zou, Minghua Chen, Yue He, Huan Liu, Min Xue, Yanfang Gu
Preeclampsia (PE), a common obstetrical disorder, is one of the leading causes of pregnancy associated death. PE is closely linked with impaired migration and invasion ability of trophoblastic cells. miR-362-3p recently received our particular attention due not only to its aberrant expression in the placentas of patients with PE, but also to its important roles in regulating migration and invasion of various cells. This study was thus conducted to investigate the roles of miR-362-3p in PE and the related mechanism...
March 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Guy R Adami, Christy C Tangney, Jessica L Tang, Yalu Zhou, Saba Ghaffari, Ankur Naqib, Saurabh Sinha, Stefan J Green, Joel L Schwartz
Consumption of green tea (GT) extracts or purified catechins has shown the ability to prevent oral and other cancers and inhibit cancer progression in rodent models, but the evidence for this in humans is mixed. Working with humans, we sought to understand the source of variable responses to GT by examining its effects on oral epithelium. Lingual epithelial RNA and lingual and gingival microbiota were measured before and after 4 weeks of exposure in tobacco smokers, whom are at high risk of oral cancer. GT consumption had on average inconsistent effects on miRNA expression in the oral epithelium...
April 12, 2018: Scientific Reports
Yuwen Wang, Heding Zhou, Xiaotian Liu, Yin Han, Suqi Pan, Yanyan Wang
BACKGROUND: The trabecular meshwork (TM) plays a critical role in the outflow of aqueous humor. OBJECTIVES: In this study, we aimed to investigate the effect of miR-181a on H2O2-induced apoptosis in TM cells. MATERIAL AND METHODS: Human primary explant-derived TM cells were cultured in fibroblast medium and then treated with different concentrations of H2O2 for 2 h. We used a series of methods to carry out the research, such as MTT assay, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR), apoptosis assay, and western blot methodology...
March 26, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
Chun Yang, Houda Tahiri, Chenrongrong Cai, Muqing Gu, Carmen Gagnon, Pierre Hardy
AIM: Excess angiogenesis or neovascularization plays a key role in the pathophysiology of several ocular diseases such as retinopathy of prematurity, diabetic retinopathy and exudative age-related macular degeneration. MicroRNA-181a (miR-181a) was found highly expressed in retina and choroidal tissues. This study intends to investigate the role of miR-181a in the regulation of ocular neovascularization in different pathophysiological conditions. METHOD: We performed the RNA sequence to identify the microRNAs components of anti-angiogenic lymphocyte-derived microparticles (LMPs)...
April 2, 2018: Cardiovascular Therapeutics
Mauro Mozael Hirsch, Janaína Brusco, Tamara Vaccaro, Rogerio Margis, Jorge E Moreira, Carmem Gottfried, Alberto A Rasia-Filho
The posterodorsal medial amygdala (MePD) is a sex steroid-sensitive and sexually dimorphic subcortical area that dynamically modulates social behaviors in rats. As different microRNA (miRNA) can act as post-transcriptional regulators of synaptic processing, we addressed changes that occur in miRNA expression in the MePD of males and females along the estrous cycle. The expression of miR25-3p, miR132-3p, miR138-5p, miR181a-5p, miR195-5p, and miR199a-5p, involved in neuronal cytoskeleton remodeling and synaptic plasticity, were evaluated by RT-qPCR...
March 28, 2018: Neuroscience
Gati K Panigrahi, Anand Ramteke, Diane Birks, Hamdy E Abouzeid Ali, Sujatha Venkataraman, Chapla Agarwal, Rajeev Vibhakar, Lance D Miller, Rajesh Agarwal, Zakaria Y Abd Elmageed, Gagan Deep
Hypoxia and expression of hypoxia-related biomarkers are associated with disease progression and treatment failure in prostate cancer (PCa). We have reported that exosomes (nanovesicles of 30-150 nm in diameter) secreted by human PCa cells under hypoxia promote invasiveness and stemness in naïve PCa cells. Here, we identified the unique microRNAs (miRNAs) loaded in exosomes secreted by PCa cells under hypoxia. Using TaqMan® array microRNA cards, we analyzed the miRNA profile in exosomes secreted by human PCa LNCaP cells under hypoxic (ExoHypoxic ) and normoxic (ExoNormoxic ) conditions...
March 2, 2018: Oncotarget
Amalia Avila Figueroa, James D Fasano, Sandra Martinez-Morilla, Srividhya Venkatesan, Gary Kupfer, Shilpa M Hattangadi
No abstract text is available yet for this article.
March 22, 2018: Haematologica
Biao Zeng, Min Zhou, Huan Wu, Zhengai Xiong
Objectives: Ovarian cancer is one of the most lethal malignant tumors in women. Secreted phosphoprotein 1 (SPP1) plays an important role in some cancer types. Therefore, the role of SPP1 in ovarian cancer was determined and the potential mechanism was elucidated. Materials and methods: The expression of SPP1 in ovarian cancer was determined by immunohistochemistry in ovarian cancer tissues and normal ovarian tissues. Cellular proliferation, migration, and invasion were determined by cell counting kit-8 assay, wound healing assay, and Matrigel invasion assay in SKOV3 and A2780 cells...
2018: OncoTargets and Therapy
Kangfeng Jiang, Shuai Guo, Tao Zhang, Yaping Yang, Gan Zhao, Aftab Shaukat, Haichong Wu, Ganzhen Deng
Acute lung injury (ALI) is a progressive clinical disease with a high mortality rate, and characterized by an excessive uncontrolled inflammatory response. MicroRNAs (miRNAs) play a critical role in various human inflammatory diseases, and have been recognized as important regulators of inflammation. However, the regulatory mechanisms mediated by miRNAs involved in Lipopolysaccharide (LPS)-induced inflammation in ALI remain hazy. In this study, we found that miR-181a expression in the lung tissues of ALI mice and LPS-stimulated RAW 264...
2018: Frontiers in Pharmacology
Yu Meng Wang, Tsz Kin Ng, Kwong Wai Choy, Hoi Kin Wong, Wai Kit Chu, Chi Pui Pang, Vishal Jhanji
PURPOSE: To illustrate the histopathology of keratoconic corneal epithelia and its micro-ribonucleic acid (miRNA) regulation. METHODS: Corneal epithelia were collected from 27 patients with keratoconus and 26 normal patients after surgery or by impression cytology. The miRNA profile was determined using miRNA microarray. The biological roles of miRNA target genes were delineated by gene ontology and pathway analyses. The expressions of significant miRNAs were validated using TaqMan polymerase chain reaction (PCR), whereas protein localization and expression of the miRNA target genes were examined by immunofluorescence and immunoblotting analyses...
March 1, 2018: Journal of Refractive Surgery
Xialu Feng, Chen Zhang, Yan Yang, Deren Hou, Anding Zhu
It has been recognized that miR-181a expression is dysregulated and intimately associated with clinical prognosis in a variety of human cancers. However, the direct role of miR-181a in tumor progression has been elusive. Moreover, mounting evidence has demonstrated that cellular apoptosis, a physiological process of programmed cell death, is disrupted in various categories of human malignancies. Multiple apoptosisrelated genes have been proven to act as the target genes of miR-181a. In this study, we hypothesize that miR-181a probably plays a potential role in modulating the procession and apoptosis of cancer cells...
February 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
Xudong Zhao, Wei Zhang, Wenyue Ji
AIM: We sought to determine the function of miR-181a/GATA6 pathway in the progression of laryngeal squamous cell carcinoma (LSCC). MATERIALS & METHODS: The expression of miR-181a and GATA6 were detected using quantitative real-time-PCR and western blotting in 127 LSCC samples and 32 corresponding control mucosa tissues. Cell death, migration and apoptosis were measured in Hep-2 cells using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), Transwell migration assay and apoptosis assay, respectively...
March 8, 2018: Future Oncology
Zhiqiong Wang, Xiaochuan Wang, Hongying Zhou, Xiao Dan, Lixiang Jiang, Yifei Wu
Melanoma is the most malignant and aggressive form of skin carcinoma originating in the pigment-producing melanocytes. In this study, to further investigate the molecular mechanisms of the development and progression of melanoma, we explored the impacts of long non-coding RNA (lncRNA) CASC2 on melanoma cell functions. Microarray analysis was carried out to identify the expression of lncRNA CASC2 in melanoma cells. MiR-181a was predicted as a sponging target of CASC2 by miRcode, while the 3'-UTR of Plexin C1 (PLXNC1) was a potential target of miR-181a according to the TargetScan database...
March 5, 2018: Acta Biochimica et Biophysica Sinica
Zhan Shi, Ying Su, Feng Wang, Ping Liu
Apoptosis of human lens epithelial (HLE) cells is a process closely associated with cataract formation. The aim of the present study was to explore the effects of microRNA (miR)‑181a against hydrogen peroxide (H2O2)-induced apoptosis in HLE cells in vitro. The recombinant lentiviral plasmid pLKO. 1‑puro‑miR‑181a was constructed and used to transfect human HLE‑B3 cells with the short hairpin (sh)RNA to silence the expression of miR‑181a. The apoptotic rate of both HLE‑B3 cells in which miR‑181a expression was stably silenced and in untransfected HLE‑B3 cells was assessed in the presence of H2O2 using flow cytometry...
February 15, 2018: Molecular Medicine Reports
Nadezhda S Geletina, Vyacheslav S Kobelev, Ekaterina V Babayants, Li Feng, Vladimir O Pustylnyak, Lyudmila F Gulyaeva
The effects of microRNAs on PTEN levels are characteristic for many types of cancer. However, the picture of the correlation between the expression levels of PTEN and its targeting microRNAs in endometrial cancer is not fully presented. Our study investigated and analysed the expression levels of PTEN and PTEN-targeting miR-21, miR-181a, miR-214, miR-301a, and miR-1908 in total of 78 samples, out of which 26 samples were from normal endometrium, whereas the 52 samples were from endometrial cancer samples. Our results demonstrated a high variability of individual endometrial cancer samples in the levels of PTEN...
February 22, 2018: Gene
Batoul Farran, Gregory Dyson, Douglas Craig, Alan Dombkowski, Jennifer L Beebe-Dimmer, Isaac J Powell, Izabela Podgorski, Lance Heilbrun, Susan Bolton, Cathryn H Bock
Prostate cancer is one of the most common cancers in men worldwide. Currently available diagnostic and prognostic tools for this disease, such as PSA, suffer from lack of specificity and sensitivity, resulting in over- and misdiagnosis. Hence, there is an urgent need for clinically relevant biomarkers capable of distinguishing between aggressive and non-aggressive forms of prostate cancer to aid in stratification, management and therapeutic decisions. To address this unmet need, we investigated the patterns of expression of a panel of 68 plasma-derived microRNAs (miRNAs) in a cohort of African American (AA) and European American (EA) prostate cancer patients (n=114)...
February 17, 2018: Carcinogenesis
Hacer Orsdemir Hortu, Emin Karaca, Betul Sozeri, Nesrin Gulez, Balahan Makay, Cumhur Gunduz, Tahir Atik, Ismihan Merve Tekin, Sevket Erbil Unsal, Cogulu Ozgur
Familial Mediterranean fever (FMF) is an inherited autoinflammatory disorder that can result in attacks with accompanying recurrent episodes of fever, serositis, and skin rash. MiRNAs are demonstrated to be associated with a number of other diseases; however, no comprehensive study has revealed its association with FMF disease. The aim is to investigate the role of microRNAs in FMF. We included 51 patients with genetically diagnosed FMF who had clinical symptoms and 49 healthy volunteers. Fifteen miRNAs that were found to be associated with autoinflammatory diseases and have a part in immune response were evaluated...
February 13, 2018: Clinical Rheumatology
S Takahara, S Y Lee, T Iwakura, K Oe, T Fukui, E Okumachi, T Waki, M Arakura, Y Sakai, K Nishida, R Kuroda, T Niikura
OBJECTIVES: Diabetes mellitus (DM) is known to impair fracture healing. Increasing evidence suggests that some microRNA (miRNA) is involved in the pathophysiology of diabetes and its complications. We hypothesized that the functions of miRNA and changes to their patterns of expression may be implicated in the pathogenesis of impaired fracture healing in DM. METHODS: Closed transverse fractures were created in the femurs of 116 rats, with half assigned to the DM group and half assigned to the control group...
February 2018: Bone & Joint Research
Jian Jiang, Xuewen Song, Jing Yang, Ke Lei, Yongan Ni, Fei Zhou, Lirong Sun
Neuroblastoma is the primary cause of cancer-related death for children one to five years of age. New therapeutic strategies and medicines are urgently needed. This study aimed to investigate the effects of triptolide (TPL), the major active component purified from Tripterygium wilfordii Hook F, on neuroblastoma SH-SY5Y cell proliferation, migration and apoptosis, as well as underlying potential mechanisms. We found that TPL inhibited SH-SY5Y cell viability, proliferation and migration but induced cell apoptosis...
February 9, 2018: Oncology Research
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