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miR-181a

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https://www.readbyqxmd.com/read/28086904/the-lncrna-crnde-promotes-colorectal-cancer-cell-proliferation-and-chemoresistance-via-mir-181a-5p-mediated-regulation-of-wnt-%C3%AE-catenin-signaling
#1
Peng Han, Jing-Wen Li, Bo-Miao Zhang, Jia-Chen Lv, Yong-Min Li, Xin-Yue Gu, Zhi-Wei Yu, Yun-He Jia, Xue-Feng Bai, Li Li, Yan-Long Liu, Bin-Bin Cui
BACKGROUND: With more than 600,000 mortalities each year, colorectal cancer (CRC) is the third most commonly diagnosed type of cancer worldwide. Recently, mechanisms involving noncoding RNAs have been implicated in the development of CRC. METHODS: We examined expression levels of lncRNA CRNDE and miR-181a-5p in 64 cases of CRC tissues and cell lines by qRT-PCR. Gain-of-function and loss-of-function assays were performed to examine the effect of CRNDE and miR-181a-5p on proliferation and chemoresistance of CRC cells...
January 13, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28077323/high-glucose-down-regulates-microrna-181a-5p-to-increase-pro-fibrotic-gene-expression-by-targeting-early-growth-response-factor-1-in-hk-2-cells
#2
Ping Xu, Mei-Ping Guan, Jian-Gang Bi, Dan Wang, Zong-Ji Zheng, Yao-Ming Xue
Tubulointerstitial fibrosis (TIF) plays an important role in the progression of renal fibrosis in diabetic nephropathy (DN). Accumulating evidence supports a crucial effect of early growth response factor 1 (Egr1) on renal fibrosis in DN, but the underlying mechanisms are not entirely clear. Here, we explored the aggravating role of Egr1 and identified microRNA-181a-5p (miR-181a-5p) as an upstream regulator of Egr1 in TIF of DN. We demonstrated that overexpression of Egr1 enhanced, whereas small interfering RNA targeting Egr1 decreased the expressions of transforming growth factor β1 (TGF-β1) and fibrosis-related genes including fibronectin and collagen I in human proximal tubule cell line (HK-2) cells...
January 7, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28053323/hbx-promotes-cell-proliferation-by-disturbing-the-cross-talk-between-mir-181a-and-pten
#3
Yi Tian, Xinqiang Xiao, Xing Gong, Feng Peng, Yun Xu, Yongfang Jiang, Guozhong Gong
Hepatitis B virus X protein (HBx) is involved in the initiation and progression of hepatocellular carcinoma (HCC). However, the mechanism is still needed to be elucidated. In this study, we explored the relationship between HBx and microRNA and their roles in hepato-carcinogenesis. Firstly, by global microarray-based microRNA profiling and qRT-PCR, we found miR-181a was strongly up-regulated in HepG2.2.15 cells (HBV positive) and pHBV1.3-expressing HepG2 cells, and HBx played a major role in it. Secondly, reduced PTEN protein expression in the presence of HBx was aslo mediated by miR-181a, and in the Luciferase reporter system, miR-181a inhibited the PTEN translation by binding the PTEN 3'-untranslated-region (UTR), and PTEN protein was decreased when epigenetic expression of miR-181a and rescued by knocking down miR-181a...
January 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28043911/mir-181a-5p-promotes-the-progression-of-gastric-cancer-via-rassf6-mediated-mapk-signalling-activation
#4
Yushuai Mi, Dongyuan Zhang, Weiliang Jiang, Junyong Weng, Chongzhi Zhou, Kejian Huang, Huamei Tang, Yang Yu, Xisheng Liu, Weiyingqi Cui, Meng Zhang, Xiaofeng Sun, Zongguang Zhou, Zhihai Peng, Senlin Zhao, Yugang Wen
We previously discovered that Ras association domain family member 6 (RASSF6) was downregulated and predicted poor prognosis in GC patients. However, the mechanisms of the down regulation of RASSF6 in GC remained unclear. Increasing evidence indicates that dysregulation of microRNAs promotes the progression of cancer through the repression of tumour suppressors. Here, we identified miR-181a-5p as a novel regulator of RASSF6 in GC. Functionally, ectopic expression or silencing of miR-181a-5p, respectively, promoted or inhibited GC cell proliferation, colony formation and cell cycle transition, as well as enhanced or prevented the invasion, metastasis of GC cells and epithelial to mesenchymal transition of GC cells in vitro and in vivo...
December 30, 2016: Cancer Letters
https://www.readbyqxmd.com/read/27999452/microrna-regulation-of-endothelial-junction-proteins-and-clinical-consequence
#5
REVIEW
Yugang Zhuang, Hu Peng, Victoria Mastej, Weiguo Chen
Cellular junctions play a critical role in structural connection and signal communication between cells in various tissues. Although there are structural and functional varieties, cellular junctions include tight junctions, adherens junctions, focal adhesion junctions, and tissue specific junctions such as PECAM-1 junctions in endothelial cells (EC), desmosomes in epithelial cells, and hemidesmosomes in EC. Cellular junction dysfunction and deterioration are indicative of clinical diseases. MicroRNAs (miRNA) are ~20 nucleotide, noncoding RNAs that play an important role in posttranscriptional regulation for almost all genes...
2016: Mediators of Inflammation
https://www.readbyqxmd.com/read/27997916/circulating-mir-181a-as-a-potential-novel-biomarker-for-diagnosis-of-acute-myocardial-infarction
#6
Jianbing Zhu, Kang Yao, Qian Wang, Junjie Guo, Hongtao Shi, Leilei Ma, Haibo Liu, Wei Gao, Yunzeng Zou, Junbo Ge
BACKGROUND: In this study, we tested the hypothesis that miR-181a levels increase during acute myocardial infarction. We investigated circulating miR-181a as a potential novel biomarker for early diagnosis of acute myocardial infarction (AMI). METHODS: From June 2014 to June 2016, 120 consecutive eligible patients with AMI (n = 60) or unstable angina (UA; n = 60) and 60 control subjects were enrolled. Plasma miR-181a levels were determined by quantitative reverse transcriptase-polymerase chain reaction...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27979502/digital-pcr-quantification-of-mir-30c-and-mir-181a-as-serum-biomarkers-for-duchenne-muscular-dystrophy
#7
Monica Llano-Diez, Carlos Ignacio Ortez, Judit Armas Gay, Lidia Álvarez-Cabado, Cristina Jou, Julita Medina, Andres Nascimento, Cecilia Jimenez-Mallebrera
Circulating microRNAs (miRs/miRNAs) are being used as non-invasive biomarkers for diagnosis, prognosis and efficiency of clinical trials. However, to exploit their potential it is necessary to improve and standardize their detection. In a previous study, we identified two microRNAs, miR-30c and miR-181a, that appear to be key regulators of muscular dystrophy. We hypothesized that they could represent useful biomarkers of Duchenne and Becker muscular dystrophies (DMD and BMD). The objective of this study was to assess the absolute levels of miR-30c and miR-181a in sera of DMD and BMD patients using digital PCR (a robust technique for precise and direct quantification of small amounts of nucleic acids without standard curves and external references), and investigate the correlation between miR-30c and miR-181a expressions and several clinical parameters...
November 11, 2016: Neuromuscular Disorders: NMD
https://www.readbyqxmd.com/read/27935974/epigallocatechin-3-gallate-upregulates-mir-221-to-inhibit-osteopontin-dependent-hepatic-fibrosis
#8
M L Arffa, M A Zapf, A N Kothari, V Chang, G N Gupta, X Ding, M M Al-Gayyar, W Syn, N M Elsherbiny, P C Kuo, Z Mi
Osteopontin (OPN) promotes hepatic fibrosis, and developing therapies targeting OPN expression in settings of hepatic injury holds promise. The polyphenol epigallocatechin-3-gallate (EGCG), found in high concentrations in green tea, downregulates OPN expression through OPN mRNA degradation, but the mechanism is unknown. Previous work has shown that microRNAs can decrease OPN mRNA levels, and other studies have shown that EGCG modulates the expression of multiple microRNAs. In our study, we first demonstrated that OPN induces hepatic stellate cells to transform into an activated state...
2016: PloS One
https://www.readbyqxmd.com/read/27915346/mir-181a-targets-phlpp2-to-augment-akt-signaling-and-regulate-proliferation-and-apoptosis-in-human-keloid-fibroblasts
#9
Zhen Rang, Zong-Yang Wang, Qiu-Yu Pang, You-Wei Wang, Ge Yang, Fan Cui
BACKGROUND/AIMS: Keloids are fibrous overgrowths induced by cutaneous injury. MicroRNAs (miRNAs) have recently emerged as post-transcriptional gene repressors and participants in a diverse array of pathophysiological processes leading to skin disease. The purpose of the current study was to explore the precise functions of miR-181a in human keloid development and the underlying mechanisms. METHODS: A miRNA microarray analysis was performed to compare expression profiles between keloid and normal skin tissues...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27911586/mirna-181a-b-regulates-phenotypes-of-vessel-smooth-muscle-cells-through-serum-response-factor
#10
Xiaoxing Wei, Xue Hou, Jianhua Li, Yongnian Liu
The phenotypic modulation of vessel smooth muscle cells (VSMCs) plays a crucial role in the physiological and pathological conditions of vasculature in response to local environmental changes. The phenotypic transition of VSMCs is largely modulated by the serum response factor (SRF). MiR-181a and miR-181b are members of the well-studied miR-181 family and both have complementary sequence in the 3' untranslated region (UTR) of SRF gene. In this article, evidence insinuates that miR-181a/b was involved in VSMCs differentiation through upregulating synthetic marker genes and downregulating contractile ones, respectively...
December 2, 2016: DNA and Cell Biology
https://www.readbyqxmd.com/read/27909056/interferons-induce-expression-of-samhd1-in-monocytes-through-down-regulation-of-mir-181a-and-mir-30a
#11
Maximilian Riess, Nina V Fuchs, Adam Idica, Matthias Hamdorf, Egbert Flory, Irene Munk Pedersen, Renate König
SAMHD1 is a phosphohydrolase maintaining cellular dNTP homeostasis but also acts as a critical regulator in innate immune responses due to its antiviral activity and association with autoimmune disease, leading to aberrant activation of interferon. SAMHD1 expression is differentially regulated by interferon in certain primary cells, but the underlying mechanism is not understood. Here, we report a detailed characterization of the promotor region, the 5'- and 3'-untranslated region (UTR) of SAMHD1, and the mechanism responsible for the cell type-dependent up-regulation of SAMHD1 protein by interferon...
January 6, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27895662/muscle-enriched-micrornas-isolated-from-whole-blood-are-regulated-by-exercise-and-are-potential-biomarkers-of-cardiorespiratory-fitness
#12
Joshua Denham, Priscilla R Prestes
MicroRNAs (miRNAs) are small non-coding RNA molecules that regulate gene expression post-transcriptionally. Evidence indicating miRNAs influence exercise-induced health and performance adaptations is mounting. Circulating miRNAs are responsible for intercellular communication and could serve as biomarkers for disease and exercise-related traits. Such biomarkers would contribute to exercise screening, monitoring, and the development of personalized exercise prescription. Accordingly, we investigated the impact of long-term strenuous aerobic exercise training and a single bout of maximal aerobic exercise on five muscle-enriched miRNAs implicated in exercise adaptations (miR-1, miR-133a, miR-181a, miR-486, and miR-494)...
2016: Frontiers in Genetics
https://www.readbyqxmd.com/read/27881005/a-microrna-expression-signature-as-a-predictor-of-survival-for-colon-adenocarcinoma
#13
M Xu, Y Kuang, M Wang, X Han, Q Yang
Colon cancer is a major cause of cancer mortality worldwide and most colon cancers are adenocarcinoma. MicroRNA (miRNA) expression signature has been shown to be able to predict progression and prognosis of various cancers. The aim of our study was to explore a novel signature of microRNA expression for predicting survival of colon adenocarcinoma patients. By analyzing the miRNA expression profiles and clinical information of 329 colon adenocarcinoma patients derived from The Cancer Genome Atlas database. 129 miRNAs were identified to be expressed differentially between the cancer and adjacent tissues...
November 24, 2016: Neoplasma
https://www.readbyqxmd.com/read/27844418/erratum-to-down-regulation-of-mir-181a-can-reduce-heat-stress-damage-in-pbmcs-of-holstein-cows
#14
Kun-Lin Chen, Yuan-Yuan Fu, Min-Yan Shi, Hui-Xia Li
No abstract text is available yet for this article.
November 14, 2016: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/27816459/microrna-21-microrna-181a-and-microrna-196a-as-potential-biomarkers-in-adult-egyptian-patients-with-systemic-lupus-erythematosus
#15
Tarek K Motawi, Dahlia A Mohsen, Shohda A El-Maraghy, Mona A Kortam
Dysregulation of miRNAs has been described in systemic lupus erythematosis (SLE), however the clinical relevance of using miRNAs as biomarkers for SLE or predictors of disease progression is poorly investigated. This study investigated the expression signature of plasma miR-21, miR-181a and miR-196a among seventy SLE patients with different systemic lupus erythematosis disease activity index (SLEDAI) scores and thirty healthy controls using quantitative real-time PCR. Plasma IL-10 level was also measured in patients and control groups...
December 25, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/27802900/downregulation-of-mir-181a-protects-mice-from-lps-induced-acute-lung-injury-by-targeting-bcl-2
#16
Wuquan Li, Xiaochen Qiu, He Jiang, Yalong Han, Dinan Wei, Jun Liu
Acute lung injury/acute respiratory distress syndrome (ALI/ARDS) is a progressive syndrome with a high incidence and mortality rate. Apoptosis is a very important pathological feature of ALI. Recently, more and more researches on mircoRNAs are involved in the regulation of proliferation and apoptosis of cancer cells. However, the functions of microRNAs in ALI remain unknown. In this study, PCR array and qRT-PCR were used to investigate microRNA profiles and miR-181a regulates apoptosis induced by LPS. Then, we predict the potential target gene of miR-181a is BCL-2 by bioinformatics methods, and validate it using luciferase reporter gene assays...
December 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27767084/mir-181a-is-a-negative-regulator-of-gria2-in-methamphetamine-use-disorder
#17
Kai Zhang, Qingzhong Wang, Xuxiu Jing, Yan Zhao, Haifeng Jiang, Jiang Du, Shunying Yu, Min Zhao
A previous study reported that the miR-181a level in serum was significantly different between patients with methamphetamine-use disorder and healthy controls and that chronic methamphetamine use down-regulates the expression of miR-181a. Bioinformatic analysis predicted that miR-181a might bind the 3'-UTRs of the mRNA transcripts of the human glutamate receptor genes GRIA2 and GABRA1. In this study, we measured the expression of GRIA2 and GABRA1 in patients with methamphetamine-use disorder. In addition, we examined whether miR-181a down-regulates GRIA2 and GABRA1 in a cell-based assay...
October 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27753829/sy-03-2-pathophysiologic-significance-of-non-coding-rna-in-hypertension
#18
Stephen Harrap
Genetic discovery in blood pressure is generally referenced in relation to protein-coding genes, despite the fact that genes less than 2% of the genome. Recent exploration of the DNA sequences between genes, once called "junk" DNA, has revealed a wealth of transcripts for RNA species that do not encode protein. These non-coding RNAs (ncRNAs) have emerged as dynamic managers of the business of the genome, able to coordinate the expression of genes in time and space to achieve the complexities of normal development and growth...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27742678/mir-181a-5p-regulates-3t3-l1-cell-adipogenesis-by-targeting-smad7-and-tcf7l2
#19
Dan Ouyang, Lifeng Xu, Lihua Zhang, Dongguang Guo, Xiaotong Tan, Xiaofang Yu, Junjie Qi, Yaqiong Ye, Qihong Liu, Yongjiang Ma, Yugu Li
MicroRNAs are highly conserved non-coding small RNAs participating in almost all kinds of biological activities. MiR-181a has been reported to be involved in the differentiation of porcine primary preadipocytes, but the profound effect of miR-181a-5p on 3T3-L1 adipocyte differentiation and proliferation is still unclear. In this study, we found that supplementation of miR-181a-5p in 3T3-L1 cells significantly promoted the adipogenesis and inhibited cell proliferation with increased expression of adipogenic marker genes including peroxisome proliferator-activated receptor gamma (Pparγ), CCAAT/enhancer-binding protein alpha (C/ebpα), fatty acid-binding protein 4 (Fabp4), and Adiponectin, accompanied by an accumulation of lipid droplet, an increase of triglyceride content, and a decrease of cell proliferation...
November 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/27699225/identification-of-microrna-181a-5p-and-microrna-4454-as-mediators-of-facet-cartilage-degeneration
#20
Akihiro Nakamura, Y Raja Rampersaud, Anirudh Sharma, Stephen J Lewis, Brian Wu, Poulami Datta, Kala Sundararajan, Helal Endisha, Evgeny Rossomacha, Jason S Rockel, Igor Jurisica, Mohit Kapoor
Osteoarthritis (OA) of spine (facet joints [FJs]) is one of the major causes of severe low back pain and disability worldwide. The degeneration of facet cartilage is a hallmark of FJ OA. However, endogenous mechanisms that initiate degeneration of facet cartilage are unknown, and there are no disease-modifying therapies to stop FJ OA. In this study, we have identified microRNAs (small noncoding RNAs) as mediators of FJ cartilage degeneration. We first established a cohort of patients with varying degrees of facet cartilage degeneration (control group: normal or mild facet cartilage degeneration; FJ OA group: moderate to severe facet cartilage degeneration) and then screened 2,100 miRNAs and identified 2 miRNAs (miR-181a-5p and miR-4454) that were significantly elevated in FJ OA cartilage compared with control facet cartilage...
August 4, 2016: JCI Insight
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