Ying Wang, Melissa Thaler, Clarisse Salgado-Benvindo, Nathan Ly, Anouk A Leijs, Dennis K Ninaber, Philip M Hansbro, Fia Boedijono, Martijn J van Hemert, Pieter S Hiemstra, Anne M van der Does, Alen Faiz
OBJECTIVES: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a member of a class of highly pathogenic coronaviruses. The large family of coronaviruses, however, also includes members that cause only mild symptoms, like human coronavirus-229E (HCoV-229E) or OC43 (HCoV-OC43). Unravelling how molecular (and cellular) pathophysiology differs between highly and low pathogenic coronaviruses is important for the development of therapeutic strategies. METHODS: Here, we analysed the transcriptome of primary human bronchial epithelial cells (PBEC), differentiated at the air-liquid interface (ALI) after infection with SARS-CoV-2, SARS-CoV, Middle East Respiratory Syndrome (MERS)-CoV and HCoV-229E using bulk RNA sequencing...
2024: Clinical & Translational Immunology